MS010325 (gene) Bitter gourd (TR) v1

Overview


Name	MS010325
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	YqgFc domain-containing protein
Location	scaffold878: 219774 .. 223362 (-)
RNA-Seq Expression	MS010325
Synteny	MS010325

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTTTCGCAACATTTTCAACCGTTTCCGCCGCAAAGCCTTATTCCGAGCAACCAGAAACTTCAGTCTTCGACCATTCTGTGGCTCCCACGATGCTCTACCTTTGCTCAGCCAATCCTAGAATGCAATTGCGTCTCAGAAACAAGGGCGATTTCCTCCATTGAACTTCCGCCTAATGCCCTTCGCCGCAAGCTCGACCCTCACTGGAGAGGAGGTTTCAGTCTAGGGGTAGACCTCGGAACCTCCCGCACTGGCCTTGCTCTTAGTAAAGGCTTCTCCACTCGTCCTCTTACCGTAATTTATCTGCTAATTTCACTATTTAATTTCCTTAAGCTCGCTTTTATTTTGTATGTCCTCACTATTTGTTGTAGAATTCGATGTCCCCAACGTTTTTGTAGTTGGTTGCTTTTCGATTTTCCGGTATCAAGGAGGAAATTCTTTCTGATAATTATAGTGGTGTTCAATGCGTTATTATGGGATATGATCGTGGAAGTCTGCTGCCATCAGAACATACGTAATGTATTTAACAAGTAAACTGTGATATGAGGTTGATGATCGATTGTTCTTACTGAATTCCGTTTCTTCTGGGACACAAGTTCTTATTAAGTTGCAAATGCAGGCTGTTATCATTTCCGTTTTATACAGTCCACCTTCGCTCTTCTTTCTAAGCTTGTTTTATGTTATACCTAAATATTGAAGTTATGAATGATTCCCAGCATTTAACTCCGTGTTGTGTGTTATCAAATATTGCGGCCTCATGATTTCTCTAGCTGGCTTAGGTCTTGGAGTTGCGAGGACATAAGCTTGAGGTTAAGCTTATTGAGATTGCTGAACAGGAAGTATGTATCCTTCCCTCTCTCATGATCCTTATACTTTCCTAATTAAGTGTTATCAACATGTTGGAACATTATTTCTTCTCTTTAGTTACATGTTAATTGATGAAAAAACTGTAGGAGGCTGATGAATTTATTATTGGACTTCCTAAATCAAGCGATGGAAAAGAGACACCGCAGTCAAATAAAATTCGTAGTATTGCTGGAAGGGTAGCAGCTCGAGCAGCTGAAAGGTGAAATACACTGAATGGGTGTACTTTATTTCCCTTATGTTTGCTTTGCTGTTCTGATACAGCTTTTAGTTTAGAGGTTGGAGAGTTTACTTGCACGATGAACATGGGACAACATCTGAAGCGGAAAACCATATGATTTGCAAGTATTGTTGTCCTCAAAACTCACTTATGTTGTCTTTTGTTTAATATTGTTAGCGTGGATTCTATTCTGCAAAACATATTGCATCATCTTTTGTTAGTATTATTTGCCTGGGTTCTGTATCGTGTAAAATTTATTGCATCACCTTCCGTTAGTGTTGTTAGTGTGGATTCTGTATCCTGTAAATAGTAGGTGGAGTAATTGCAACCGACTATTTCCGGAGGATGTTTGGGATGGTGGCATTGTCAGGTGGGCTTTTATGTTTAGAAAGAATTTGTTGGACTAGATGAATGGTTTAACCTCTGCCTCATTCTTGATAATTTTTCATCGTTTTAACCTTTCTCAAGGTAGATGAATGGTTTAACCTCTGTCTCATGCCTCATTCTTGATAATTTTTCATCGTTTTAACCTTTCTCTAGGTAGATGAATGGTTTAACCTCTGCCTCATTCTTGATAATTTTTCATCGTTTTAACCTTTCTCTAGGTAGATGAATAGTTTAACCTCTGCCTCATTCTTGATAATTTTTCATCGTTTTAACCACATCAACCTAGATAAATATTCATGTAAAAAGACGAACGGCCACATCTTCATGGATTGTCGTTTCTTGGAAGTTTGTGAACTCCCCCCACTTCCAATCTACTTGGCCTTTACTTTATTCCTTTAGAGGGAAGTCATTTTCTGAAACCGAAGAGAGAGCTTATTTGGTGTTGTGGCATCTTCGCTATTTTTGGGGAAAGTATCATTAAAATCAAATTAGACTTCCTTATGCATTTTCCACTTTTTATGACTACCTATTTTCTATTATCTACGCCACTAGGATAGCCTTTTTGTTTGAAACCACTTTGCTTTGGGGGCACTGCCGTCTGTATATATTTCATTTTGATCAATGAAATTTGGTGGTTCTTTTTTTTTAACCGTCTGCTTTTGGAGGAGTTAGGGAACTGTGCTTCAATGGAAGAGTGACCTATAATATTAGCATAAGGAGTGGTGAACTGTGGCTTGAGTTTAGGAGTGATCAAGATAATCCCTCAATTACTTTATTTTATGAGAAATCAAACTTTCATTGAGAAATATGAAAGGAAAAATAAGAGCATACAAAAAATAAAATAAAATAGACCCTCAAAAGGAATCCAAACTAACAAACAGACTCCAATCTGAAATCTCTATCATCATAAGATTCCAACTTACGTAGAGTGAGTGGGCCACTAGAGACTAGTGCCAACAAAGCCATTTACTCAAGGAAAGGAGATTTCATATGGATGGTAGGAAGACAATAAGAGGGTCCGGGTACAAAGTTGTACCAACCAACTCTGACACCAAGGTTTGAGGTTACCATCTCTGTTGACAAGATTTAACGAACTTCAAAAGAAAAGATAAGCTGGTAAACATTTGTTTCCTCTCTGATAGTTTCACCGTACAATTGTCTCCATGGAAAGCAATTGAGAACGTCTATTGATTTTATGCCTGCGACATAGTAAGAAGACACTCAATGCTAGAACAACCAAAAAGAAACCAAATTGTGACAGAGGGGGAGAAAAGCAAGTAAGATCAAATGATGAGGACGATGGAGAACCTAGTCAATTACTCTAATTTTGATTCTCATGTAAACTTCTTTGTGAAACATGGAGAATTTTGACTTGTTTCTTTTAAGGTTGTTGCATGGATGGTTACACATTTTCGTCTCTAGTAGTCACTGATGTTCTCAGTGTTCTTTTGCTTTGTGGTATTTAATGGAAGAATAGAATTGAAAATATTTTGTGATTAAAATGGACCTGAAGCACTGCAGCTGTTCTTAGATTTTTCTTTATGCAAATTTAGTTTGGTTGCATTGATGCTTTGTTCCATCCAGAAAATTAAAATTTTATTATCCCACCAGTTTGGAAAAGACTACTGATTATCTTTTAGATACTATTTCGTTTGTGGCTTTCTCTTAAGATAAATTATATGTACCCTGTCGCTTCAACAACTATGGTTAAAATGTTCTTTCATCAATTAGAACTTTTTATAATCTTTTCTGGCTCGGGGTTAGCTTTCCTCCCCTCTTTTGTAATTCCATATCATCTATCACATTGGTTTTTGTACTTTTCATGTTTTCCCCAGGGGGCTCAATAAATCTACTCGACAGAAGAAAATCGATGCCTATGCTGCCATGGTAAAGTGCTGATTCTCATACTTATGATCATTGGATGATGACTGCAGCAATGAATGTCAGGTACATATCTTTCCGCTCATAATTTTCACTAGTTTTACTTTATTGATGCAGATGGTACTAGAGAGGTATTATTTCATGTCAGGCCAGGGAACCGAACTTGTAGTACCTAAGAGTCTAGTCTTACAAGAAAAACTTATCGAGGGACCACCTACAGACCCTGACTTTAAGGAT

mRNA sequence

ATGTTTTCGCAACATTTTCAACCGTTTCCGCCGCAAAGCCTTATTCCGAGCAACCAGAAACTTCAGTCTTCGACCATTCTGTGGCTCCCACGATGCTCTACCTTTGCTCAGCCAATCCTAGAATGCAATTGCGTCTCAGAAACAAGGGCGATTTCCTCCATTGAACTTCCGCCTAATGCCCTTCGCCGCAAGCTCGACCCTCACTGGAGAGGAGGTTTCAGTCTAGGGGTAGACCTCGGAACCTCCCGCACTGGCCTTGCTCTTAGTAAAGGCTTCTCCACTCGTCCTCTTACCGTCTTGGAGTTGCGAGGACATAAGCTTGAGGTTAAGCTTATTGAGATTGCTGAACAGGAAGAGGCTGATGAATTTATTATTGGACTTCCTAAATCAAGCGATGGAAAAGAGACACCGCAGTCAAATAAAATTCGTAGTATTGCTGGAAGGGTAGCAGCTCGAGCAGCTGAAAGAGGTTGGAGAGTTTACTTGCACGATGAACATGGGACAACATCTGAAGCGGAAAACCATATGATTTGCAAGGGGCTCAATAAATCTACTCGACAGAAGAAAATCGATGCCTATGCTGCCATGATGGTACTAGAGAGGTATTATTTCATGTCAGGCCAGGGAACCGAACTTGTAGTACCTAAGAGTCTAGTCTTACAAGAAAAACTTATCGAGGGACCACCTACAGACCCTGACTTTAAGGAT

Coding sequence (CDS)

ATGTTTTCGCAACATTTTCAACCGTTTCCGCCGCAAAGCCTTATTCCGAGCAACCAGAAACTTCAGTCTTCGACCATTCTGTGGCTCCCACGATGCTCTACCTTTGCTCAGCCAATCCTAGAATGCAATTGCGTCTCAGAAACAAGGGCGATTTCCTCCATTGAACTTCCGCCTAATGCCCTTCGCCGCAAGCTCGACCCTCACTGGAGAGGAGGTTTCAGTCTAGGGGTAGACCTCGGAACCTCCCGCACTGGCCTTGCTCTTAGTAAAGGCTTCTCCACTCGTCCTCTTACCGTCTTGGAGTTGCGAGGACATAAGCTTGAGGTTAAGCTTATTGAGATTGCTGAACAGGAAGAGGCTGATGAATTTATTATTGGACTTCCTAAATCAAGCGATGGAAAAGAGACACCGCAGTCAAATAAAATTCGTAGTATTGCTGGAAGGGTAGCAGCTCGAGCAGCTGAAAGAGGTTGGAGAGTTTACTTGCACGATGAACATGGGACAACATCTGAAGCGGAAAACCATATGATTTGCAAGGGGCTCAATAAATCTACTCGACAGAAGAAAATCGATGCCTATGCTGCCATGATGGTACTAGAGAGGTATTATTTCATGTCAGGCCAGGGAACCGAACTTGTAGTACCTAAGAGTCTAGTCTTACAAGAAAAACTTATCGAGGGACCACCTACAGACCCTGACTTTAAGGAT

Protein sequence

MFSQHFQPFPPQSLIPSNQKLQSSTILWLPRCSTFAQPILECNCVSETRAISSIELPPNALRRKLDPHWRGGFSLGVDLGTSRTGLALSKGFSTRPLTVLELRGHKLEVKLIEIAEQEEADEFIIGLPKSSDGKETPQSNKIRSIAGRVAARAAERGWRVYLHDEHGTTSEAENHMICKGLNKSTRQKKIDAYAAMMVLERYYFMSGQGTELVVPKSLVLQEKLIEGPPTDPDFKD

Homology

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BLAST of MS010325 vs. NCBI nr
Match: XP_022144406.1 (uncharacterized protein LOC111014097 isoform X1 [Momordica charantia])

HSP 1 Score: 474.9 bits (1221), Expect = 3.9e-130
Identity = 236/236 (100.00%), Postives = 236/236 (100.00%), Query Frame = 0


Query: 1   MFSQHFQPFPPQSLIPSNQKLQSSTILWLPRCSTFAQPILECNCVSETRAISSIELPPNA 60
           MFSQHFQPFPPQSLIPSNQKLQSSTILWLPRCSTFAQPILECNCVSETRAISSIELPPNA
Sbjct: 1   MFSQHFQPFPPQSLIPSNQKLQSSTILWLPRCSTFAQPILECNCVSETRAISSIELPPNA 60

Query: 61  LRRKLDPHWRGGFSLGVDLGTSRTGLALSKGFSTRPLTVLELRGHKLEVKLIEIAEQEEA 120
           LRRKLDPHWRGGFSLGVDLGTSRTGLALSKGFSTRPLTVLELRGHKLEVKLIEIAEQEEA
Sbjct: 61  LRRKLDPHWRGGFSLGVDLGTSRTGLALSKGFSTRPLTVLELRGHKLEVKLIEIAEQEEA 120

Query: 121 DEFIIGLPKSSDGKETPQSNKIRSIAGRVAARAAERGWRVYLHDEHGTTSEAENHMICKG 180
           DEFIIGLPKSSDGKETPQSNKIRSIAGRVAARAAERGWRVYLHDEHGTTSEAENHMICKG
Sbjct: 121 DEFIIGLPKSSDGKETPQSNKIRSIAGRVAARAAERGWRVYLHDEHGTTSEAENHMICKG 180

Query: 181 LNKSTRQKKIDAYAAMMVLERYYFMSGQGTELVVPKSLVLQEKLIEGPPTDPDFKD 237
           LNKSTRQKKIDAYAAMMVLERYYFMSGQGTELVVPKSLVLQEKLIEGPPTDPDFKD
Sbjct: 181 LNKSTRQKKIDAYAAMMVLERYYFMSGQGTELVVPKSLVLQEKLIEGPPTDPDFKD 236

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BLAST of MS010325 vs. NCBI nr
Match: XP_022144417.1 (uncharacterized protein LOC111014097 isoform X2 [Momordica charantia])

HSP 1 Score: 410.2 bits (1053), Expect = 1.2e-110
Identity = 213/236 (90.25%), Postives = 213/236 (90.25%), Query Frame = 0


Query: 1   MFSQHFQPFPPQSLIPSNQKLQSSTILWLPRCSTFAQPILECNCVSETRAISSIELPPNA 60
           MFSQHFQPFPPQSLIPSNQKLQSSTILWLPRCSTFAQPILECNCVSETRAISSIELPPNA
Sbjct: 1   MFSQHFQPFPPQSLIPSNQKLQSSTILWLPRCSTFAQPILECNCVSETRAISSIELPPNA 60

Query: 61  LRRKLDPHWRGGFSLGVDLGTSRTGLALSKGFSTRPLTVLELRGHKLEVKLIEIAEQEEA 120
           LRRKLDPHWRGGFSLGVDLGTSRTGLALSKGFSTRPLTVLELRGHKLEVKLIEIAEQEEA
Sbjct: 61  LRRKLDPHWRGGFSLGVDLGTSRTGLALSKGFSTRPLTVLELRGHKLEVKLIEIAEQEEA 120

Query: 121 DEFIIGLPKSSDGKETPQSNKIRSIAGRVAARAAERGWRVYLHDEHGTTSEAENHMICKG 180
           DEFIIGLPKSSDGKETPQSNKIRSIAGRVAARAAER                       G
Sbjct: 121 DEFIIGLPKSSDGKETPQSNKIRSIAGRVAARAAER-----------------------G 180

Query: 181 LNKSTRQKKIDAYAAMMVLERYYFMSGQGTELVVPKSLVLQEKLIEGPPTDPDFKD 237
           LNKSTRQKKIDAYAAMMVLERYYFMSGQGTELVVPKSLVLQEKLIEGPPTDPDFKD
Sbjct: 181 LNKSTRQKKIDAYAAMMVLERYYFMSGQGTELVVPKSLVLQEKLIEGPPTDPDFKD 213

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BLAST of MS010325 vs. NCBI nr
Match: XP_022144424.1 (uncharacterized protein LOC111014097 isoform X3 [Momordica charantia])

HSP 1 Score: 395.6 bits (1015), Expect = 3.0e-106
Identity = 196/197 (99.49%), Postives = 197/197 (100.00%), Query Frame = 0


Query: 1   MFSQHFQPFPPQSLIPSNQKLQSSTILWLPRCSTFAQPILECNCVSETRAISSIELPPNA 60
           MFSQHFQPFPPQSLIPSNQKLQSSTILWLPRCSTFAQPILECNCVSETRAISSIELPPNA
Sbjct: 1   MFSQHFQPFPPQSLIPSNQKLQSSTILWLPRCSTFAQPILECNCVSETRAISSIELPPNA 60

Query: 61  LRRKLDPHWRGGFSLGVDLGTSRTGLALSKGFSTRPLTVLELRGHKLEVKLIEIAEQEEA 120
           LRRKLDPHWRGGFSLGVDLGTSRTGLALSKGFSTRPLTVLELRGHKLEVKLIEIAEQEEA
Sbjct: 61  LRRKLDPHWRGGFSLGVDLGTSRTGLALSKGFSTRPLTVLELRGHKLEVKLIEIAEQEEA 120

Query: 121 DEFIIGLPKSSDGKETPQSNKIRSIAGRVAARAAERGWRVYLHDEHGTTSEAENHMICKG 180
           DEFIIGLPKSSDGKETPQSNKIRSIAGRVAARAAERGWRVYLHDEHGTTSEAENHMICKG
Sbjct: 121 DEFIIGLPKSSDGKETPQSNKIRSIAGRVAARAAERGWRVYLHDEHGTTSEAENHMICKG 180

Query: 181 LNKSTRQKKIDAYAAMM 198
           LNKSTRQKKIDAYAAM+
Sbjct: 181 LNKSTRQKKIDAYAAMV 197

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BLAST of MS010325 vs. NCBI nr
Match: XP_038893103.1 (putative pre-16S rRNA nuclease isoform X1 [Benincasa hispida])

HSP 1 Score: 367.9 bits (943), Expect = 6.8e-98
Identity = 193/237 (81.43%), Postives = 203/237 (85.65%), Query Frame = 0


Query: 4   QHFQPFPPQSLIPSNQKLQSSTILWLPRCSTFA----QPILECNCVSETRAISSIELPPN 63
           Q FQPFP QS      K+   TI  LPR  + A     P L C C    +AISSI+LPPN
Sbjct: 7   QEFQPFPLQS-----HKVHCPTIFCLPRIPSLAPLPPSPTLICKC---QKAISSIDLPPN 66

Query: 64  ALRRKLDPHWRGGFSLGVDLGTSRTGLALSKGFSTRPLTVLELRGHKLEVKLIEIAEQEE 123
           ALRRKLDP WRGGFSLGVDLGTSRTGLALSKGFSTRPL VLELRG KLEVKLIEIAEQEE
Sbjct: 67  ALRRKLDPQWRGGFSLGVDLGTSRTGLALSKGFSTRPLGVLELRGQKLEVKLIEIAEQEE 126

Query: 124 ADEFIIGLPKSSDGKETPQSNKIRSIAGRVAARAAERGWRVYLHDEHGTTSEAENHMICK 183
           ADEFIIGLPKS DGKETPQSNKIRSIAGRVA RAAERGWRVYLHDEHGTT+EAE+HMI K
Sbjct: 127 ADEFIIGLPKSCDGKETPQSNKIRSIAGRVATRAAERGWRVYLHDEHGTTAEAESHMISK 186

Query: 184 GLNKSTRQKKIDAYAAMMVLERYYFMSGQGTELVVPKSLVLQEKLIEGPPTDPDFKD 237
           GLNKSTRQKKIDAYAAMMVLERY+FMSG+GTELVVPKSL+LQ+KLIEGPPTDPDFKD
Sbjct: 187 GLNKSTRQKKIDAYAAMMVLERYFFMSGEGTELVVPKSLILQDKLIEGPPTDPDFKD 235

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BLAST of MS010325 vs. NCBI nr
Match: XP_023528266.1 (uncharacterized protein LOC111791236 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 366.7 bits (940), Expect = 1.5e-97
Identity = 193/240 (80.42%), Postives = 204/240 (85.00%), Query Frame = 0


Query: 1   MFSQHFQPFPPQSLIPSNQKLQSSTILWLPRCSTF----AQPILECNCVSETRAISSIEL 60
           M + HFQPFPPQS      KL SS I   PR S+       P L C C +   AISS+EL
Sbjct: 1   MCALHFQPFPPQS-----HKLHSSAIFCPPRPSSLPLLPPSPNLICKCKT---AISSVEL 60

Query: 61  PPNALRRKLDPHWRGGFSLGVDLGTSRTGLALSKGFSTRPLTVLELRGHKLEVKLIEIAE 120
           PPNALRRKLDP WRGGFSLGVDLGTSRTGLALSKGFSTRPLTVLELRG KLE KLIEIAE
Sbjct: 61  PPNALRRKLDPQWRGGFSLGVDLGTSRTGLALSKGFSTRPLTVLELRGQKLEFKLIEIAE 120

Query: 121 QEEADEFIIGLPKSSDGKETPQSNKIRSIAGRVAARAAERGWRVYLHDEHGTTSEAENHM 180
           QEEADEFIIGLPKS DGKETPQSNKIRSIAGRVAA+AAERGWRVYLHDEHGTT+EAE+HM
Sbjct: 121 QEEADEFIIGLPKSLDGKETPQSNKIRSIAGRVAAQAAERGWRVYLHDEHGTTAEAESHM 180

Query: 181 ICKGLNKSTRQKKIDAYAAMMVLERYYFMSGQGTELVVPKSLVLQEKLIEGPPTDPDFKD 237
           IC+GLNK+TRQKKIDAYAAMMVLERY+ MSG+GTELVVPK  VLQEKLIEGPP DPDFKD
Sbjct: 181 ICRGLNKTTRQKKIDAYAAMMVLERYFSMSGEGTELVVPKGQVLQEKLIEGPPIDPDFKD 232

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BLAST of MS010325 vs. ExPASy Swiss-Prot
Match: A1SJC8 (Putative pre-16S rRNA nuclease OS=Nocardioides sp. (strain ATCC BAA-499 / JS614) OX=196162 GN=Noca_2408 PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.1e-10
Identity = 50/137 (36.50%), Postives = 74/137 (54.01%), Query Frame = 0


Query: 70  RGGFSLGVDLGTSRTGLALS--KGFSTRPL-TVLELRGHKLEVKLIEIAEQEEAD---EF 129
           R G  +G+D G +R G+A S   GF   P+ TV   +G    +  I  AE++E     E 
Sbjct: 2   RAGVRIGIDPGDARIGVARSDPSGFLATPVETVRRGKGDLARIGRILAAEEDEGATVLEV 61

Query: 130 IIGLPKSSDGKETPQSNKIRSIAGRVAARAAERGWRVYLHDEHGTTSEAENHMICKGLNK 189
           ++GLP+S  G+E P + K+R  AGR+AAR A     V L DE  TT  AE  +  +G   
Sbjct: 62  VVGLPRSLSGREGPAAAKVREFAGRLAARVAP--VPVRLVDERMTTVSAEAMLRDQGRTG 121

Query: 190 STRQKKIDAYAAMMVLE 201
             R+  +D  AA+++L+
Sbjct: 122 GKRRAVVDQAAAVLILQ 136

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BLAST of MS010325 vs. ExPASy Swiss-Prot
Match: B8DIJ5 (Putative pre-16S rRNA nuclease OS=Desulfovibrio vulgaris (strain DSM 19637 / Miyazaki F) OX=883 GN=DvMF_2352 PE=3 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 1.0e-08
Identity = 40/131 (30.53%), Postives = 71/131 (54.20%), Query Frame = 0


Query: 75  LGVDLGTSRTGLALSK--GFSTRP-LTVLELRGHKLEVKLIEIAEQEEADEFIIGLPKSS 134
           LG+D GT RTG+A S   G    P  T++     +   +L+ +AE+E A+ +++GLP   
Sbjct: 4   LGIDYGTKRTGVAASDTGGSMAFPRRTIVMTTRDRFFAELLAVAEEERAEAYVVGLPLLH 63

Query: 135 DGKETPQSNKIRSIAGRVAARAAERGWRVYLHDEHGTTSEAENHMICKGLNKSTRQKKID 194
           DG +T  + ++R+   R+  R       VYL +E  ++ EAE+ +   GL+    +  +D
Sbjct: 64  DGTDTLTTRQVRNFVERLKRRTT---LPVYLMEEFLSSYEAEDDLRDAGLSGRALEAVVD 123

Query: 195 AYAAMMVLERY 203
             AA+ +L+ +
Sbjct: 124 QQAAVRILQSF 131

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BLAST of MS010325 vs. ExPASy Swiss-Prot
Match: Q3SR28 (Putative pre-16S rRNA nuclease OS=Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255) OX=323098 GN=Nwi_2004 PE=3 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.4e-08
Identity = 44/137 (32.12%), Postives = 74/137 (54.01%), Query Frame = 0


Query: 68  HWRG-GFSLGVDLGTSRTGLALS---KGFSTRPLTVLELRGHKLEVKLIEIAEQEEADEF 127
           HW   G  +G+DLGT   G+A+S   +  +T   T+          +L+ IA +  A+ F
Sbjct: 14  HWPARGALVGLDLGTKTIGVAVSDPDRRLATGVETIRRKTFTADAARLLAIAGERRAEGF 73

Query: 128 IIGLPKSSDGKETPQSNKIRSIAGRVAARAAERGWRVYLHDEHGTTSEAENHMICKGLNK 187
           I+GLP + DG E P++   R+ A R  AR  +  + + L DE  +T+  E  +I   +++
Sbjct: 74  ILGLPINMDGSEGPRAQSTRAFA-RNLARLTD--FAIGLWDERLSTAAVERELIGLDMSR 133

Query: 188 STRQKKIDAYAAMMVLE 201
           + R K ID +AA+ +L+
Sbjct: 134 ARRAKVIDEHAAIFILQ 147

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BLAST of MS010325 vs. ExPASy Swiss-Prot
Match: Q215X7 (Putative pre-16S rRNA nuclease OS=Rhodopseudomonas palustris (strain BisB18) OX=316056 GN=RPC_2255 PE=3 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 2.3e-08
Identity = 42/132 (31.82%), Postives = 71/132 (53.79%), Query Frame = 0


Query: 72  GFSLGVDLGTSRTGLALS---KGFSTRPLTVLELRGHKLEVKLIEIAEQEEADEFIIGLP 131
           G  +G+DLGT   G+A+S   +  +T   T++         +L+ +A Q  A  F++GLP
Sbjct: 19  GALIGLDLGTKTIGVAVSDPDRRLATGVETIIHTAFKANAARLLLLAGQRNAVGFVLGLP 78

Query: 132 KSSDGKETPQSNKIRSIAGRVAARAAERGWRVYLHDEHGTTSEAENHMICKGLNKSTRQK 191
            + DG E P++   R+ A R  AR  +    + L DE  +TS  E  +I   ++++ R K
Sbjct: 79  INMDGSEGPRAQSTRAFA-RNFARLTD--LPIGLWDERLSTSAVERELIANDVSRAKRAK 138

Query: 192 KIDAYAAMMVLE 201
            ID +AA+ +L+
Sbjct: 139 VIDEHAAIFILQ 147

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BLAST of MS010325 vs. ExPASy Swiss-Prot
Match: C6C125 (Putative pre-16S rRNA nuclease OS=Desulfovibrio salexigens (strain ATCC 14822 / DSM 2638 / NCIMB 8403 / VKM B-1763) OX=526222 GN=Desal_1125 PE=3 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 4.0e-08
Identity = 42/131 (32.06%), Postives = 70/131 (53.44%), Query Frame = 0


Query: 75  LGVDLGTSRTGLALS--KGFSTRPLTVLE-LRGHKLEVKLIEIAEQEEADEFIIGLPKSS 134
           L +D GT R GLA+S        P  V+E      +  +L+EI E E+  + +IGLP S 
Sbjct: 4   LSIDFGTKRVGLAISDPDQIFAFPYKVMERTTRDAMFSELLEIIENEKVGDIVIGLPLSL 63

Query: 135 DGKETPQSNKIRSIAGRVAARAAERGWRVYLHDEHGTTSEAENHMICKGLNKSTRQKKID 194
           DG++T  + ++R+ A  +  R       ++L DE  ++  AE+ +   GL    R+K +D
Sbjct: 64  DGEDTLTTRQVRNFAASLERRV---DLPIHLVDERLSSIAAEDELKEAGLWDRKRKKNLD 123

Query: 195 AYAAMMVLERY 203
           + AA ++LE +
Sbjct: 124 SQAAKIILETW 131

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BLAST of MS010325 vs. ExPASy TrEMBL
Match: A0A6J1CTB8 (uncharacterized protein LOC111014097 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111014097 PE=3 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 1.9e-130
Identity = 236/236 (100.00%), Postives = 236/236 (100.00%), Query Frame = 0


Query: 1   MFSQHFQPFPPQSLIPSNQKLQSSTILWLPRCSTFAQPILECNCVSETRAISSIELPPNA 60
           MFSQHFQPFPPQSLIPSNQKLQSSTILWLPRCSTFAQPILECNCVSETRAISSIELPPNA
Sbjct: 1   MFSQHFQPFPPQSLIPSNQKLQSSTILWLPRCSTFAQPILECNCVSETRAISSIELPPNA 60

Query: 61  LRRKLDPHWRGGFSLGVDLGTSRTGLALSKGFSTRPLTVLELRGHKLEVKLIEIAEQEEA 120
           LRRKLDPHWRGGFSLGVDLGTSRTGLALSKGFSTRPLTVLELRGHKLEVKLIEIAEQEEA
Sbjct: 61  LRRKLDPHWRGGFSLGVDLGTSRTGLALSKGFSTRPLTVLELRGHKLEVKLIEIAEQEEA 120

Query: 121 DEFIIGLPKSSDGKETPQSNKIRSIAGRVAARAAERGWRVYLHDEHGTTSEAENHMICKG 180
           DEFIIGLPKSSDGKETPQSNKIRSIAGRVAARAAERGWRVYLHDEHGTTSEAENHMICKG
Sbjct: 121 DEFIIGLPKSSDGKETPQSNKIRSIAGRVAARAAERGWRVYLHDEHGTTSEAENHMICKG 180

Query: 181 LNKSTRQKKIDAYAAMMVLERYYFMSGQGTELVVPKSLVLQEKLIEGPPTDPDFKD 237
           LNKSTRQKKIDAYAAMMVLERYYFMSGQGTELVVPKSLVLQEKLIEGPPTDPDFKD
Sbjct: 181 LNKSTRQKKIDAYAAMMVLERYYFMSGQGTELVVPKSLVLQEKLIEGPPTDPDFKD 236

[Close]

BLAST of MS010325 vs. ExPASy TrEMBL
Match: A0A6J1CTM3 (uncharacterized protein LOC111014097 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111014097 PE=4 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 5.8e-111
Identity = 213/236 (90.25%), Postives = 213/236 (90.25%), Query Frame = 0


Query: 1   MFSQHFQPFPPQSLIPSNQKLQSSTILWLPRCSTFAQPILECNCVSETRAISSIELPPNA 60
           MFSQHFQPFPPQSLIPSNQKLQSSTILWLPRCSTFAQPILECNCVSETRAISSIELPPNA
Sbjct: 1   MFSQHFQPFPPQSLIPSNQKLQSSTILWLPRCSTFAQPILECNCVSETRAISSIELPPNA 60

Query: 61  LRRKLDPHWRGGFSLGVDLGTSRTGLALSKGFSTRPLTVLELRGHKLEVKLIEIAEQEEA 120
           LRRKLDPHWRGGFSLGVDLGTSRTGLALSKGFSTRPLTVLELRGHKLEVKLIEIAEQEEA
Sbjct: 61  LRRKLDPHWRGGFSLGVDLGTSRTGLALSKGFSTRPLTVLELRGHKLEVKLIEIAEQEEA 120

Query: 121 DEFIIGLPKSSDGKETPQSNKIRSIAGRVAARAAERGWRVYLHDEHGTTSEAENHMICKG 180
           DEFIIGLPKSSDGKETPQSNKIRSIAGRVAARAAER                       G
Sbjct: 121 DEFIIGLPKSSDGKETPQSNKIRSIAGRVAARAAER-----------------------G 180

Query: 181 LNKSTRQKKIDAYAAMMVLERYYFMSGQGTELVVPKSLVLQEKLIEGPPTDPDFKD 237
           LNKSTRQKKIDAYAAMMVLERYYFMSGQGTELVVPKSLVLQEKLIEGPPTDPDFKD
Sbjct: 181 LNKSTRQKKIDAYAAMMVLERYYFMSGQGTELVVPKSLVLQEKLIEGPPTDPDFKD 213

[Close]

BLAST of MS010325 vs. ExPASy TrEMBL
Match: A0A6J1CS97 (uncharacterized protein LOC111014097 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111014097 PE=4 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 1.5e-106
Identity = 196/197 (99.49%), Postives = 197/197 (100.00%), Query Frame = 0


Query: 1   MFSQHFQPFPPQSLIPSNQKLQSSTILWLPRCSTFAQPILECNCVSETRAISSIELPPNA 60
           MFSQHFQPFPPQSLIPSNQKLQSSTILWLPRCSTFAQPILECNCVSETRAISSIELPPNA
Sbjct: 1   MFSQHFQPFPPQSLIPSNQKLQSSTILWLPRCSTFAQPILECNCVSETRAISSIELPPNA 60

Query: 61  LRRKLDPHWRGGFSLGVDLGTSRTGLALSKGFSTRPLTVLELRGHKLEVKLIEIAEQEEA 120
           LRRKLDPHWRGGFSLGVDLGTSRTGLALSKGFSTRPLTVLELRGHKLEVKLIEIAEQEEA
Sbjct: 61  LRRKLDPHWRGGFSLGVDLGTSRTGLALSKGFSTRPLTVLELRGHKLEVKLIEIAEQEEA 120

Query: 121 DEFIIGLPKSSDGKETPQSNKIRSIAGRVAARAAERGWRVYLHDEHGTTSEAENHMICKG 180
           DEFIIGLPKSSDGKETPQSNKIRSIAGRVAARAAERGWRVYLHDEHGTTSEAENHMICKG
Sbjct: 121 DEFIIGLPKSSDGKETPQSNKIRSIAGRVAARAAERGWRVYLHDEHGTTSEAENHMICKG 180

Query: 181 LNKSTRQKKIDAYAAMM 198
           LNKSTRQKKIDAYAAM+
Sbjct: 181 LNKSTRQKKIDAYAAMV 197

[Close]

BLAST of MS010325 vs. ExPASy TrEMBL
Match: A0A6J1IVR0 (uncharacterized protein LOC111479048 OS=Cucurbita maxima OX=3661 GN=LOC111479048 PE=3 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 1.4e-96
Identity = 192/240 (80.00%), Postives = 202/240 (84.17%), Query Frame = 0


Query: 1   MFSQHFQPFPPQSLIPSNQKLQSSTILWLPRCSTF----AQPILECNCVSETRAISSIEL 60
           M + HFQPFP QS      KL SS I   PR S+       P L C C     AISS+EL
Sbjct: 1   MCALHFQPFPLQS-----HKLHSSAIFCPPRPSSLPLLPPSPNLICKC---RTAISSVEL 60

Query: 61  PPNALRRKLDPHWRGGFSLGVDLGTSRTGLALSKGFSTRPLTVLELRGHKLEVKLIEIAE 120
           PPNALRRKLDP WRGGFSLGVDLGTSRTGLALSKGFSTRPLTVLELRG KLE KLIEIAE
Sbjct: 61  PPNALRRKLDPQWRGGFSLGVDLGTSRTGLALSKGFSTRPLTVLELRGQKLEFKLIEIAE 120

Query: 121 QEEADEFIIGLPKSSDGKETPQSNKIRSIAGRVAARAAERGWRVYLHDEHGTTSEAENHM 180
           QEEADEFIIGLPKS DGKETPQSNKIRSIAGRVAA+AAERGWRVYLHDEHGTT+EAE+HM
Sbjct: 121 QEEADEFIIGLPKSLDGKETPQSNKIRSIAGRVAAQAAERGWRVYLHDEHGTTAEAESHM 180

Query: 181 ICKGLNKSTRQKKIDAYAAMMVLERYYFMSGQGTELVVPKSLVLQEKLIEGPPTDPDFKD 237
           IC+GLNK+TRQKKIDAYAAMMVLERY+ MSG+GTELVVPK  VLQEKLIEGPP DPDFKD
Sbjct: 181 ICRGLNKTTRQKKIDAYAAMMVLERYFSMSGEGTELVVPKGQVLQEKLIEGPPIDPDFKD 232

[Close]

BLAST of MS010325 vs. ExPASy TrEMBL
Match: A0A6J1EA74 (uncharacterized protein LOC111432185 OS=Cucurbita moschata OX=3662 GN=LOC111432185 PE=3 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 4.0e-96
Identity = 191/240 (79.58%), Postives = 202/240 (84.17%), Query Frame = 0


Query: 1   MFSQHFQPFPPQSLIPSNQKLQSSTILWLPRCSTF----AQPILECNCVSETRAISSIEL 60
           M + HFQPFP QS      KL SS I   PR S+       P L C C +   AISS+EL
Sbjct: 1   MCALHFQPFPLQS-----DKLHSSAIFCPPRPSSLPLLPPSPNLICKCKT---AISSVEL 60

Query: 61  PPNALRRKLDPHWRGGFSLGVDLGTSRTGLALSKGFSTRPLTVLELRGHKLEVKLIEIAE 120
           PPNALRRKLDP WRGGFSLGVDLGTSRTGLALSKGFSTRPLTVLELRG KLE KLIEIAE
Sbjct: 61  PPNALRRKLDPQWRGGFSLGVDLGTSRTGLALSKGFSTRPLTVLELRGQKLEFKLIEIAE 120

Query: 121 QEEADEFIIGLPKSSDGKETPQSNKIRSIAGRVAARAAERGWRVYLHDEHGTTSEAENHM 180
           QEEADEFIIGLPKS DGKETPQSNKIRSIAGRVAA+AAERGWRVYLHDEHGTT+EAE+HM
Sbjct: 121 QEEADEFIIGLPKSLDGKETPQSNKIRSIAGRVAAQAAERGWRVYLHDEHGTTAEAESHM 180

Query: 181 ICKGLNKSTRQKKIDAYAAMMVLERYYFMSGQGTELVVPKSLVLQEKLIEGPPTDPDFKD 237
           IC+GLNK+ RQKKIDAYAAMMVLERY+ MSG+GTELVVPK  VLQEKLIEGPP DPDFKD
Sbjct: 181 ICRGLNKTARQKKIDAYAAMMVLERYFSMSGEGTELVVPKGQVLQEKLIEGPPIDPDFKD 232

[Close]

BLAST of MS010325 vs. TAIR 10
Match: AT1G12244.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 261.5 bits (667), Expect = 6.4e-70
Identity = 127/186 (68.28%), Postives = 158/186 (84.95%), Query Frame = 0


Query: 50  AISSI-ELPPNALRRKLDPHWRGGFSLGVDLGTSRTGLALSKGFSTRPLTVLELRGHKLE 109
           A++SI E+PPNA+RRK+D +WRGGFSLGVDLG SRTG+A+SKG++ +PLTVL+ RG KLE
Sbjct: 58  AVTSIDEIPPNAVRRKIDRNWRGGFSLGVDLGLSRTGIAISKGYTVKPLTVLKSRGQKLE 117

Query: 110 VKLIEIAEQEEADEFIIGLPKSSDGKETPQSNKIRSIAGRVAARAAERGWRVYLHDEHGT 169
            +L+EIAE+EEADEFIIGLP+SSDGKET QSNKIRS+AGR+A +AAERGWRVY+ DEHGT
Sbjct: 118 TRLLEIAEEEEADEFIIGLPRSSDGKETIQSNKIRSVAGRLAIQAAERGWRVYVFDEHGT 177

Query: 170 TSEAENHMICKGLNKSTRQKKIDAYAAMMVLERYYFMSGQGTELVVPKSLVLQEKLIEGP 229
           TSEA + MI  GL+KS RQ + DAYAA+++LERY+   G G E+++PKSL LQ K+  G 
Sbjct: 178 TSEASDRMIVMGLSKSERQNRSDAYAAVILLERYFSTQGLGIEIILPKSLELQAKIKNGA 237

Query: 230 PTDPDF 235
           P DPDF
Sbjct: 238 PVDPDF 243

The following BLAST results are available for this feature:

BLAST of MS010325 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022144406.1	3.9e-130	100.00	uncharacterized protein LOC111014097 isoform X1 [Momordica charantia]	[more]
XP_022144417.1	1.2e-110	90.25	uncharacterized protein LOC111014097 isoform X2 [Momordica charantia]	[more]
XP_022144424.1	3.0e-106	99.49	uncharacterized protein LOC111014097 isoform X3 [Momordica charantia]	[more]
XP_038893103.1	6.8e-98	81.43	putative pre-16S rRNA nuclease isoform X1 [Benincasa hispida]	[more]
XP_023528266.1	1.5e-97	80.42	uncharacterized protein LOC111791236 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS010325 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A1SJC8	1.1e-10	36.50	Putative pre-16S rRNA nuclease OS=Nocardioides sp. (strain ATCC BAA-499 / JS614)...	[more]
B8DIJ5	1.0e-08	30.53	Putative pre-16S rRNA nuclease OS=Desulfovibrio vulgaris (strain DSM 19637 / Miy...	[more]
Q3SR28	1.4e-08	32.12	Putative pre-16S rRNA nuclease OS=Nitrobacter winogradskyi (strain ATCC 25391 / ...	[more]
Q215X7	2.3e-08	31.82	Putative pre-16S rRNA nuclease OS=Rhodopseudomonas palustris (strain BisB18) OX=...	[more]
C6C125	4.0e-08	32.06	Putative pre-16S rRNA nuclease OS=Desulfovibrio salexigens (strain ATCC 14822 / ...	[more]

BLAST of MS010325 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CTB8	1.9e-130	100.00	uncharacterized protein LOC111014097 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1CTM3	5.8e-111	90.25	uncharacterized protein LOC111014097 isoform X2 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1CS97	1.5e-106	99.49	uncharacterized protein LOC111014097 isoform X3 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1IVR0	1.4e-96	80.00	uncharacterized protein LOC111479048 OS=Cucurbita maxima OX=3661 GN=LOC111479048...	[more]
A0A6J1EA74	4.0e-96	79.58	uncharacterized protein LOC111432185 OS=Cucurbita moschata OX=3662 GN=LOC1114321...	[more]

BLAST of MS010325 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G12244.1	6.4e-70	68.28	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006641	YqgF/RNase H-like domain	SMART	SM00732	rnase_8s	coord: 72..172 e-value: 4.2E-18 score: 76.1
IPR037027	YqgF/RNase H-like domain superfamily	GENE3D	3.30.420.140		coord: 74..210 e-value: 3.5E-31 score: 110.0
IPR005227	Putative pre-16S rRNA nuclease	PFAM	PF03652	RuvX	coord: 74..202 e-value: 9.1E-27 score: 94.1
IPR005227	Putative pre-16S rRNA nuclease	TIGRFAM	TIGR00250	TIGR00250	coord: 75..203 e-value: 3.4E-19 score: 67.2
IPR005227	Putative pre-16S rRNA nuclease	PANTHER	PTHR33317	POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN	coord: 35..234
IPR005227	Putative pre-16S rRNA nuclease	HAMAP	MF_00651	Nuclease_YqgF	coord: 72..207 score: 16.156252
IPR005227	Putative pre-16S rRNA nuclease	CDD	cd16964	YqgF	coord: 75..202 e-value: 3.59715E-38 score: 127.604
None	No IPR available	PANTHER	PTHR33317:SF4	POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN	coord: 35..234
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 73..202

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS010325.1	MS010325.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006139	nucleobase-containing compound metabolic process
biological_process	GO:0006364	rRNA processing