Homology
BLAST of MS010323 vs. NCBI nr
Match:
XP_022144189.1 (vacuolar protein sorting-associated protein 8 homolog [Momordica charantia])
HSP 1 Score: 3498.8 bits (9071), Expect = 0.0e+00
Identity = 1799/1947 (92.40%), Postives = 1803/1947 (92.60%), Query Frame = 0
Query: 1 MTKELTDTGVLPLMELDLNAFIHAHLSSSDDDDDDDPSFPHRTIDEILNESGSAPSSPPH 60
MTKELTDTGVLPLMELDLNAFIHAHLSS DDDDDDDPSFPHRTIDEIL ESGS PSSPPH
Sbjct: 1 MTKELTDTGVLPLMELDLNAFIHAHLSSGDDDDDDDPSFPHRTIDEILKESGSPPSSPPH 60
Query: 61 SPPYSGDRAVATRDGAVSESPSTPPSKSPFEAPRSNQWNEKSVQLRPAAVSLVKVGELTD 120
SPPY GDRAVATRDG
Sbjct: 61 SPPYGGDRAVATRDG--------------------------------------------- 120
Query: 121 DLFRRGSRPLPSLFGGVRSNAKPGAALAAAAAASRSMPAPHAAAIKSRRAGHGSMVIDDD 180
Sbjct: 121 ------------------------------------------------------------ 180
Query: 181 ELASPSADDSEAVFGGLYSTNTHSEESGENSTVMDDTDADYQVASINVGGGVWARDRIRD 240
NSTVMDDTDADYQVASINVG GVWARDRIRD
Sbjct: 181 -----------------------------NSTVMDDTDADYQVASINVGDGVWARDRIRD 240
Query: 241 DVPDNDEFRITKGLKCVAEQRSVDDVNFDESTTILPPVEANDGSVSDYADKNVCSTELRP 300
DVPDNDEFRITKGLKCVAEQRSVDDVNFDESTTILPPVEANDGSVSDYA+KNVCSTELRP
Sbjct: 241 DVPDNDEFRITKGLKCVAEQRSVDDVNFDESTTILPPVEANDGSVSDYAEKNVCSTELRP 300
Query: 301 SILDVDESNEGAIPSSSEPDNDGSAVDYGSLEWETQDFKKCLQQSKDTEVDLVIKGPSVV 360
SILDVDESNEGAIPSSSEPDNDGSAVDYGSLEWETQDFKKCLQQSKDTEVDLVIK PSVV
Sbjct: 301 SILDVDESNEGAIPSSSEPDNDGSAVDYGSLEWETQDFKKCLQQSKDTEVDLVIKDPSVV 360
Query: 361 NDISELGETTEHMDDSTISEQAEMMLLPSTNPLEAAEEIEKKQAFTALHWEEGVAAQPMK 420
NDISELGETTEHMDDSTISEQAEMMLLPSTNPLEAAEEIEKKQAFTALHWEEGVAAQPMK
Sbjct: 361 NDISELGETTEHMDDSTISEQAEMMLLPSTNPLEAAEEIEKKQAFTALHWEEGVAAQPMK 420
Query: 421 LEGIKGGSTTLGYFDIQADNSISRTISSHSFKREHGFPQVLAVHANYIAVGMSKGNIVVV 480
LEGIKGGSTTLGYFDIQADNSISRTISSHSFKREHGFPQVLAVHANYIAVGMSKGNIVVV
Sbjct: 421 LEGIKGGSTTLGYFDIQADNSISRTISSHSFKREHGFPQVLAVHANYIAVGMSKGNIVVV 480
Query: 481 ASKYSAQNGDNMDAKMLLLGLQGDKSTAPVTSLCFNLQGDLLLAGYNDGHITVWDVLRAS 540
ASKYSAQNGDNMDAKMLLLGLQGDKSTAPVTSLCFNLQGDLLLAGYNDGHITVWDVLRAS
Sbjct: 481 ASKYSAQNGDNMDAKMLLLGLQGDKSTAPVTSLCFNLQGDLLLAGYNDGHITVWDVLRAS 540
Query: 541 AAKIISGEHTSPVVHSLFLGQDAQVTRQFKAVTGDSKGLVLLHTFSVVPLLNRFSIKTQC 600
AAKIISGEHTSPVVHSLFLGQDAQVTRQFKAVTGDSKGLVLLHTFSVVPLLNRFSIKTQC
Sbjct: 541 AAKIISGEHTSPVVHSLFLGQDAQVTRQFKAVTGDSKGLVLLHTFSVVPLLNRFSIKTQC 600
Query: 601 LLDGQKTGTVLSASALLLNESCGSSLPPTLANVAVSTSSIGSMMGGVVGGDSGWKLFNEG 660
LLDGQKTGTVLSASALLLNESCGSSLPPTLANVAVSTSSIGSMMGGVVGGDSGWKLFNEG
Sbjct: 601 LLDGQKTGTVLSASALLLNESCGSSLPPTLANVAVSTSSIGSMMGGVVGGDSGWKLFNEG 660
Query: 661 SSLVEEGVVIFATHQTALVVRLSPTLEVYAQLSKPDGVREGSMPYTAWKCSQSFETSPSE 720
SSLVEEGVVIFATHQTALVVRLSPTLEVYAQLSKPDGVREGSMPYTAWKCSQSFETSPSE
Sbjct: 661 SSLVEEGVVIFATHQTALVVRLSPTLEVYAQLSKPDGVREGSMPYTAWKCSQSFETSPSE 720
Query: 721 AVERVSLLAIAWDKMVQVAKLVKAELKVCGKWSLESAAIGVAWLDDQVLVVLTITGQLFL 780
AVERVSLLAIAWDKMVQVAKLVKAELKVCGKWSLESAAIGVAWLDDQVLVVLTITGQLFL
Sbjct: 721 AVERVSLLAIAWDKMVQVAKLVKAELKVCGKWSLESAAIGVAWLDDQVLVVLTITGQLFL 780
Query: 781 FEKDGTMIHQTSVFVDGFDKEDFIAYHTHFVNVLGNPEKAYHNCVAVRGASIYVLGPMHL 840
FEKDGTMIHQTSVFVDGFDKEDFIAYHTHFVNVLGNPEKAYHNCVAVRGAS+YVLGPMHL
Sbjct: 781 FEKDGTMIHQTSVFVDGFDKEDFIAYHTHFVNVLGNPEKAYHNCVAVRGASVYVLGPMHL 840
Query: 841 IISRLLPWKERVQVLRKAGDWMGALSMAITIYDGQAHGVIDLPRSLESLQELVVPFLIEL 900
IISRLLPWKERVQVLRKAGDWMGALSMAITIYDGQAHGVIDLPRSLESLQELVVPFLIEL
Sbjct: 841 IISRLLPWKERVQVLRKAGDWMGALSMAITIYDGQAHGVIDLPRSLESLQELVVPFLIEL 900
Query: 901 LLSYVDEVFSYISVAFCNQIEKNEKSVDVTSGGHSAHSEIKEQYNRVGGVAVEFCVHIRR 960
LLSYVDEVFSYISVAFCNQIEKNEKSVDVTSGGHSAHSEIKEQYNRVGGVAVEFCVHIRR
Sbjct: 901 LLSYVDEVFSYISVAFCNQIEKNEKSVDVTSGGHSAHSEIKEQYNRVGGVAVEFCVHIRR 960
Query: 961 TDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKGWLQRVEQ 1020
TDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKGWLQRVEQ
Sbjct: 961 TDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKGWLQRVEQ 1020
Query: 1021 CVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLQTSKSERASA 1080
CVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLQTSKSERASA
Sbjct: 1021 CVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLQTSKSERASA 1080
Query: 1081 IGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQPLRDELLQFLLENSDSVDTRSISNKSSEI 1140
IGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQ LRDELLQFLLENSDSVDTRSISNKSSEI
Sbjct: 1081 IGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSDSVDTRSISNKSSEI 1140
Query: 1141 GYLKLYHLLELDTGATLDVLRCAFVEGEIPKTNSSPNGLDDVSMQVQEEKNSTSGRKNVL 1200
GYLKLYHLLELDTGATLDVLRCAFVEGEIPKTNSSPNGLDDVSMQVQEEKNSTSGRKNVL
Sbjct: 1141 GYLKLYHLLELDTGATLDVLRCAFVEGEIPKTNSSPNGLDDVSMQVQEEKNSTSGRKNVL 1200
Query: 1201 IQSIVDALVHVLDQAICQTDRSAGGEDIALVGDWPSKKDLVHLFDFVANYVACGRATASK 1260
IQSIVDALVHVLDQAICQTDRSAGGEDIALVGDWPSKKDLVHLFDFVANYVACG ATASK
Sbjct: 1201 IQSIVDALVHVLDQAICQTDRSAGGEDIALVGDWPSKKDLVHLFDFVANYVACGGATASK 1260
Query: 1261 VVVGQILEHLISNSDIPETENDFLPKVTANSVHSRKREKQVLSLLEVVPETHWNASSVLS 1320
V+VGQILEHLISNSDIPETENDFLPKVTANSVHSRKREKQVLSLLEVVPETHWNASSVLS
Sbjct: 1261 VIVGQILEHLISNSDIPETENDFLPKVTANSVHSRKREKQVLSLLEVVPETHWNASSVLS 1320
Query: 1321 MCEEAQFFQVCGLIHSIRHQYASALDSYMKDIDEPIHGFAFINRTLLELNNYERTEFQAV 1380
MCEEAQFFQVCGLIHSIRHQYASALDSYMKDIDEPIHGFAFINRTLLELNNYERTEFQAV
Sbjct: 1321 MCEEAQFFQVCGLIHSIRHQYASALDSYMKDIDEPIHGFAFINRTLLELNNYERTEFQAV 1380
Query: 1381 VISRIPELFNLNREGTFFLVIDHFNNDASDILSQLRTHPRSLFLYLKTLIEVHLSGSLNF 1440
VISRIPELFNLNREGTFFLVIDHFNNDASDILSQLRTHPRSLFLYLKTLIEVHLSGSLNF
Sbjct: 1381 VISRIPELFNLNREGTFFLVIDHFNNDASDILSQLRTHPRSLFLYLKTLIEVHLSGSLNF 1440
Query: 1441 SCLRKDDIFGLNYSTKGLDDYLRKLSDFPKYLSNNPVDVTDDIIELYVELLCQYERESVL 1500
SCLRKDDIFGLNYSTKGLDDYLRKLSDFPKYLSNNPVDVTDDIIELYVELLCQYERESVL
Sbjct: 1441 SCLRKDDIFGLNYSTKGLDDYLRKLSDFPKYLSNNPVDVTDDIIELYVELLCQYERESVL 1500
Query: 1501 KFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDKKFNDLEAAVE 1560
KFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDKKFNDLEAAVE
Sbjct: 1501 KFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDKKFNDLEAAVE 1560
Query: 1561 SIVANGASSGSNDPRHFNSVLKLQEVNDIDVLLHACIGLCQRNTPRLNSEESETLWFKLL 1620
SIVANGASSGSNDPRHFNSVLKLQEVNDIDVLLHACIGLCQRNTPRLNSEESETLWFKLL
Sbjct: 1561 SIVANGASSGSNDPRHFNSVLKLQEVNDIDVLLHACIGLCQRNTPRLNSEESETLWFKLL 1620
Query: 1621 DSFCEPLIDSYNYRTPAFGENQVQFLNESSGLQKDKEARIVTWRILKSDKAAHILKRLFS 1680
DSFCEPLIDSYNYRTPAFGENQVQFLNESSGLQKDKEARIVTWRILKSDKAAHILKRLFS
Sbjct: 1621 DSFCEPLIDSYNYRTPAFGENQVQFLNESSGLQKDKEARIVTWRILKSDKAAHILKRLFS 1680
Query: 1681 RFIKEIVEGMIGYVHLPIIMSRLLSDNGSQEFGDFKLTVLGMLGTFGFERRILDTAKALI 1740
RFIKEIVEGMIGYVHLPIIMSRLLSDNGSQEFGDFKLTVLGMLGTFGFERRILDTAKALI
Sbjct: 1681 RFIKEIVEGMIGYVHLPIIMSRLLSDNGSQEFGDFKLTVLGMLGTFGFERRILDTAKALI 1740
Query: 1741 EDDTFYTMSLLKKGASHGYAPRNLVCCICNCLLIKSSSSYRVRVFNCGHATHLQCEVLDN 1800
EDDTFYTMSLLKKGASHGYAPRNLVCCICNCLLIKSSSSYRVRVFNCGHATHLQCEVLDN
Sbjct: 1741 EDDTFYTMSLLKKGASHGYAPRNLVCCICNCLLIKSSSSYRVRVFNCGHATHLQCEVLDN 1800
Query: 1801 EASGGDFSCPVCIHSNQSQRSRGKALTEYSLVNTFSSGTQSLSGASVQYPQETDLLELPY 1860
EASGGDFSCPVCIHSNQSQR RGKALTEYSLVNTFSSGTQSLSGASVQYPQETDLLELPY
Sbjct: 1801 EASGGDFSCPVCIHSNQSQRPRGKALTEYSLVNTFSSGTQSLSGASVQYPQETDLLELPY 1813
Query: 1861 TLQQIPRFEILTNLQKNQRVIEIENVPQLRLAPPAVYHDKVTKGYHLLVGESSSGIEKQN 1920
TLQQIPRFEILTNLQKNQRVIEIENVPQLRLAPPAVYHDKVTKGYHLLVGESSSGIEKQN
Sbjct: 1861 TLQQIPRFEILTNLQKNQRVIEIENVPQLRLAPPAVYHDKVTKGYHLLVGESSSGIEKQN 1813
Query: 1921 KSKQLREVKVKRRSSLRFPLKASLFGE 1948
KSKQLREVKVKR SSLRFPLKASLFG+
Sbjct: 1921 KSKQLREVKVKRPSSLRFPLKASLFGK 1813
BLAST of MS010323 vs. NCBI nr
Match:
XP_038883953.1 (vacuolar protein sorting-associated protein 8 homolog isoform X1 [Benincasa hispida])
HSP 1 Score: 3269.2 bits (8475), Expect = 0.0e+00
Identity = 1676/1960 (85.51%), Postives = 1773/1960 (90.46%), Query Frame = 0
Query: 1 MTKELTDTGVLPLMELDLNAFIHAHLSS---SDDDDDDDPSFPHRTIDEILNESGSAPSS 60
MTKELTD LPLMELDLNAFIHAHLSS D DDDDD SFPHR+IDEILNES S+ SS
Sbjct: 31 MTKELTDNETLPLMELDLNAFIHAHLSSGDGDDGDDDDDLSFPHRSIDEILNESSSSTSS 90
Query: 61 PPHSPPYSGDRAVATRDGAVSESPSTPPSKSPF-------EAPRSNQWNEKSVQLRPAAV 120
PPHSPP G R A DG VS SPS P KSPF E PRSNQWNEKSVQL+P AV
Sbjct: 91 PPHSPPSRGLRNNAAGDGGVSTSPSISPFKSPFEETIKDPETPRSNQWNEKSVQLKPGAV 150
Query: 121 SLVKVGELTDDLFRRGSRPLPSLFGGVRSNAKPGAALAAAAAASRSMPAPHAAAIKSRRA 180
S KVGELTDD FRRGSRPLPSLFGGVRSNAKPGAALAAAAAASRS PAPHAAAIKSRRA
Sbjct: 151 SHAKVGELTDDPFRRGSRPLPSLFGGVRSNAKPGAALAAAAAASRSTPAPHAAAIKSRRA 210
Query: 181 GHGSMVIDDDELASPSADDSEAVFGGLYSTNTHSEESGENSTVMDDTDADYQVASINVGG 240
G+G+MV+DDDEL S SA +SE LY TN HSE+SGE S M D ADYQVAS+NVGG
Sbjct: 211 GYGNMVLDDDELVSSSAVESEFFSDSLYHTNIHSEDSGEKSISMVDRIADYQVASMNVGG 270
Query: 241 GVWARDRIRDDVPDNDEFRITKGLKCVAEQRSVDDVNFDESTTILPPVEANDGSVSDYAD 300
+W RD I D VP NDEF IT+ ++C AE SVDDV+F+ES T LP V AN+ S+S
Sbjct: 271 ELWERDNIGDGVPYNDEFLITRDMECEAEPSSVDDVDFNESATTLPRVGANNRSLSGPVQ 330
Query: 301 KNVCSTELRPSILDVDESNEGAIPSSSEPDNDGSAVDYGSLEWETQDFKKCLQQSKDTEV 360
KNVCS E P+ LDVDESNEGAIP S EPD++GS+V YGSLE ETQDF+KCLQ SKDTEV
Sbjct: 331 KNVCSMETHPTALDVDESNEGAIPWSIEPDDEGSSVGYGSLELETQDFEKCLQPSKDTEV 390
Query: 361 DLVIKGPSVVNDISELGETTEHMDDSTISEQAEMMLLPSTNPLEAAEEIEKKQAFTALHW 420
DL I+ PS V+DI ELGETTE +D+ I E+ E M L STNPLE AEEIEKKQAFTALHW
Sbjct: 391 DLAIEDPSEVDDIIELGETTEQLDNFQIGERPERMPLSSTNPLELAEEIEKKQAFTALHW 450
Query: 421 EEGVAAQPMKLEGIKGGSTTLGYFDIQADNSISRTISSHSFKREHGFPQVLAVHANYIAV 480
EEGVAAQPM+LEGIKGG+TTLGYFDIQADNSISRTISSHSFKREHGF QVLAVHANYIAV
Sbjct: 451 EEGVAAQPMRLEGIKGGTTTLGYFDIQADNSISRTISSHSFKREHGFSQVLAVHANYIAV 510
Query: 481 GMSKGNIVVVASKYSAQNGDNMDAKMLLLGLQGDKSTAPVTSLCFNLQGDLLLAGYNDGH 540
GMSKGNIVVVASK SAQNGDNMDAKMLLLG QGDKSTAPVTSLCFN QGDLLLAGY+DGH
Sbjct: 511 GMSKGNIVVVASKSSAQNGDNMDAKMLLLGSQGDKSTAPVTSLCFNQQGDLLLAGYSDGH 570
Query: 541 ITVWDVLRASAAKIISGEHTSPVVHSLFLGQDAQVTRQFKAVTGDSKGLVLLHTFSVVPL 600
ITVWDVLRASAAK+ISGEHTSPVVHSLFLGQ+AQVTRQFKAVTGDSKGLVLLHTFSVVPL
Sbjct: 571 ITVWDVLRASAAKVISGEHTSPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFSVVPL 630
Query: 601 LNRFSIKTQCLLDGQKTGTVLSASALLLNESCGSSLPPTLANVAVSTSSIGSMMGGVVGG 660
LNRFS KTQCLLDGQKTGTVLSASALLLNE CGSSLPPTL+N AVSTSSIGSMMGGVVGG
Sbjct: 631 LNRFSNKTQCLLDGQKTGTVLSASALLLNEFCGSSLPPTLSNAAVSTSSIGSMMGGVVGG 690
Query: 661 DSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSPTLEVYAQLSKPDGVREGSMPYTAWKC 720
DSGWKLFNEGSS VEEGVV+FATHQTALVVRLSPT+EVYAQLSKPDG+REGSMPYTAWKC
Sbjct: 691 DSGWKLFNEGSSFVEEGVVVFATHQTALVVRLSPTVEVYAQLSKPDGIREGSMPYTAWKC 750
Query: 721 SQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKAELKVCGKWSLESAAIGVAWLDDQVLV 780
SQSFETSPSEAVERVSLLAIAWDKMVQVAKLVK ELKVCGKWSLESAAIGV WL+DQVLV
Sbjct: 751 SQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVWLNDQVLV 810
Query: 781 VLTITGQLFLFEKDGTMIHQTSVFVDGFDKEDFIAYHTHFVNVLGNPEKAYHNCVAVRGA 840
+LT+TGQLFLFEKDGTMIHQTS+FVDGFDKEDFIAYHTHFVNVLGNPEKAYHNCVAVRGA
Sbjct: 811 ILTVTGQLFLFEKDGTMIHQTSIFVDGFDKEDFIAYHTHFVNVLGNPEKAYHNCVAVRGA 870
Query: 841 SIYVLGPMHLIISRLLPWKERVQVLRKAGDWMGALSMAITIYDGQAHGVIDLPRSLESLQ 900
SIYVLGPMHL+ISRLLPWKERVQVLRKAGDWM ALSMAITIYDG AHGVIDLPRSLESLQ
Sbjct: 871 SIYVLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSLESLQ 930
Query: 901 ELVVPFLIELLLSYVDEVFSYISVAFCNQIEKNEKSVDVTSGGHSAHSEIKEQYNRVGGV 960
ELV+PFLIELLLSYVDEVFSYISVAFCNQIEKNEK DV S H AHSEIKEQYNRVGGV
Sbjct: 931 ELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDVMSESHYAHSEIKEQYNRVGGV 990
Query: 961 AVEFCVHIRRTDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYS 1020
AVEFCVHI RTDILFDEIFSKFV VQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYS
Sbjct: 991 AVEFCVHITRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYS 1050
Query: 1021 HKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVL 1080
HKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVL
Sbjct: 1051 HKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVL 1110
Query: 1081 QTSKSERASAIGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQPLRDELLQFLLENSDSVDT 1140
+TSKS+RAS++GYKTLVYLKYCFSGLAFPPGQGTLAHSRVQ LRDELLQFLLENSD VDT
Sbjct: 1111 RTSKSKRASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSDPVDT 1170
Query: 1141 RSISNKSSEIGYLKLYHLLELDTGATLDVLRCAFVEGEIPKTNSSPNGLDDVSMQVQEEK 1200
RSISNKSSE+GYL LYHLLELDTGATLDVLRCAFVE E+ KTNSSP+G D MQ++EEK
Sbjct: 1171 RSISNKSSEVGYLNLYHLLELDTGATLDVLRCAFVEDEVLKTNSSPDGSVDARMQLREEK 1230
Query: 1201 NSTSGRKNVLIQSIVDALVHVLDQAICQTDRSAGGEDIALVGDWPSKKDLVHLFDFVANY 1260
NS SGRKN LIQ++VD L+HVLD+AIC+TD S G++I LV +WPSKKDL+HLFDFVA Y
Sbjct: 1231 NSISGRKNFLIQNVVDGLIHVLDKAICETDGSQAGDNITLVNNWPSKKDLIHLFDFVATY 1290
Query: 1261 VACGRATASKVVVGQILEHLISNSDIPETENDFLPKVTANSVHSRKREKQVLSLLEVVPE 1320
VACG+ATASK VVGQILEHLIS SDI ET +D LP VTANSVHSRKREKQVLSLLEVVPE
Sbjct: 1291 VACGKATASKDVVGQILEHLISKSDIAETTSDCLPHVTANSVHSRKREKQVLSLLEVVPE 1350
Query: 1321 THWNASSVLSMCEEAQFFQVCGLIHSIRHQYASALDSYMKDIDEPIHGFAFINRTLLELN 1380
THWN SSVL MCE+AQFFQVCGLIHSIR QY+SALD YMKD+DEPIH F FINR LLEL+
Sbjct: 1351 THWNPSSVLRMCEKAQFFQVCGLIHSIRRQYSSALDGYMKDLDEPIHAFVFINRALLELS 1410
Query: 1381 NYERTEFQAVVISRIPELFNLNREGTFFLVIDHFNNDASDILSQLRTHPRSLFLYLKTLI 1440
N ERTEF+AVVIS+IPELFNLNRE TFFLVIDHFNN+ SDILSQL HPRSLFLYLKTLI
Sbjct: 1411 NSERTEFRAVVISQIPELFNLNREATFFLVIDHFNNEVSDILSQLHNHPRSLFLYLKTLI 1470
Query: 1441 EVHLSGSLNFSCLRKDDIFGLNYSTKGLDDYLRKLSDFPKYLSNNPVDVTDDIIELYVEL 1500
EVHLSGSL+FSCL+KD FG+NYSTKGLDDYL+KLSDFPKYLSNNPVDVTDDIIELYV+L
Sbjct: 1471 EVHLSGSLDFSCLKKDGNFGVNYSTKGLDDYLQKLSDFPKYLSNNPVDVTDDIIELYVKL 1530
Query: 1501 LCQYERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDK 1560
LCQYERESVLKFLETFDSY VEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDK
Sbjct: 1531 LCQYERESVLKFLETFDSYHVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDK 1590
Query: 1561 KFNDLEAAVESIVANGASSGSNDPRHFNSVLKLQEVNDIDVLLHACIGLCQRNTPRLNSE 1620
KF+DLEAAV IV NGASSGSND +HF+SVLKLQEV ++VLLHACIGLCQRNTPRLNSE
Sbjct: 1591 KFHDLEAAVGGIVTNGASSGSNDSQHFSSVLKLQEVKAVEVLLHACIGLCQRNTPRLNSE 1650
Query: 1621 ESETLWFKLLDSFCEPLIDSYNYRTPAFGENQVQFLNESSGLQKDKEARIVTWRILKSDK 1680
ESETLWFKLLDSFCEPLIDSYNYRT +F ENQVQFLNESS QK KEA IVTWRILKS++
Sbjct: 1651 ESETLWFKLLDSFCEPLIDSYNYRTASFEENQVQFLNESSASQKAKEAHIVTWRILKSNE 1710
Query: 1681 AAHILKRLFSRFIKEIVEGMIGYVHLPIIMSRLLSDNGSQEFGDFKLTVLGMLGTFGFER 1740
AAHIL+RLFS+FI+EIVEGM GYVHLP IMSRLL DNGSQEFGDFKLT+LGMLGTFGFER
Sbjct: 1711 AAHILRRLFSQFIREIVEGMTGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTFGFER 1770
Query: 1741 RILDTAKALIEDDTFYTMSLLKKGASHGYAPRNLVCCICNCLLIKSSSSYRVRVFNCGHA 1800
RILDTAK LIEDDTFYTMSLLKKGASHGYAP+++VCCICN LLIKSSSSYRVRVFNCGHA
Sbjct: 1771 RILDTAKTLIEDDTFYTMSLLKKGASHGYAPQSVVCCICNRLLIKSSSSYRVRVFNCGHA 1830
Query: 1801 THLQCEVLDNEASGGDFSCPVCIHSNQSQRSRGKALTEYSLVNTFSSGTQSLSGASVQYP 1860
THLQCE DNEASGGDF CP+C+HSNQSQRSR KA +EY LVN FSS TQS SGASV YP
Sbjct: 1831 THLQCEDPDNEASGGDFICPICVHSNQSQRSRSKAPSEYGLVNKFSSRTQSSSGASVSYP 1890
Query: 1861 QETDLLELPYTLQQIPRFEILTNLQKNQRVIEIENVPQLRLAPPAVYHDKVTKGYHLLVG 1920
QETDLLELPYTLQQIPRFEILTNLQKNQRVI+IENVPQLRLAPP VYHDKVTKGYHLLVG
Sbjct: 1891 QETDLLELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPTVYHDKVTKGYHLLVG 1950
Query: 1921 ESSSG---IEKQNKSKQLREVKVKRRSSLRFPLKASLFGE 1948
ESSSG +E+ NKS+QL EVKVKR SSLRFPLKASLFG+
Sbjct: 1951 ESSSGKEKVERLNKSRQLTEVKVKRPSSLRFPLKASLFGK 1990
BLAST of MS010323 vs. NCBI nr
Match:
KAG6582725.1 (Vacuolar protein sorting-associated protein 8-like protein, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 3260.3 bits (8452), Expect = 0.0e+00
Identity = 1680/1964 (85.54%), Postives = 1780/1964 (90.63%), Query Frame = 0
Query: 1 MTKELTDTGVLPLMELDLNAFIHAHLSSSDDDDDDDP---SFPHRTIDEILNES----GS 60
MTKELTDT LP MELDLNAFIHAHLSS DDDDDDD SFPHR+IDEILNES S
Sbjct: 1 MTKELTDTETLPPMELDLNAFIHAHLSSGDDDDDDDEDDLSFPHRSIDEILNESSSSTSS 60
Query: 61 APSSPPHSPPYSGDRAVATRDGAVSESPSTPPSKSPFE-------APRSNQWNEKSVQLR 120
+PSSPP+SPP R++A RDG S S S PP KSPFE PRSNQ NEKSVQL+
Sbjct: 61 SPSSPPNSPPPRDRRSIAARDGRASASRSIPPFKSPFEEIIKASKVPRSNQRNEKSVQLK 120
Query: 121 PAAVSLVKVGELTDDLFRRGSRPLPSLFGGVRSNAKPGAALAAAAAASRSMPAPHAAAIK 180
P + S KVGELTDD FRRGSR LPSLFGGVRSNAKPGAALAAAAAASRSMPAPHAAAIK
Sbjct: 121 PGSASHTKVGELTDDPFRRGSRALPSLFGGVRSNAKPGAALAAAAAASRSMPAPHAAAIK 180
Query: 181 SRRAGHGSMVIDDDELASPSADDSEAVFGGLYSTNTHSEESGENSTVMDDTDADYQVASI 240
SRR+GHGS+V+DDDELAS SA DSE VF LY T HS+ES E S + + +ADYQ AS+
Sbjct: 181 SRRSGHGSVVLDDDELASSSAVDSEFVFDDLYPTIDHSKESREKSISLVERNADYQGASV 240
Query: 241 NVGGGVWARDRIRDDVPDNDEFRITKGLKCVAEQRSVDDVNFDESTTILPPVEANDGSVS 300
NVGG WARD IRD V NDEFRITK +C AEQ VDDVNFDES+T LPPVEAN S+S
Sbjct: 241 NVGGEFWARDNIRDCVLYNDEFRITKDTECEAEQSFVDDVNFDESSTTLPPVEANGRSLS 300
Query: 301 DYADKNVCSTELRPSILDVDESNEGAIPSSSEPDNDGSAVDYGSLEWETQDFKKCLQQSK 360
D AD NVCS + P++LDVDESNEGA P S +PD D SAV YG LE ETQDF+K Q SK
Sbjct: 301 DSADNNVCSMDAEPTVLDVDESNEGAFPCSPKPDYDRSAVGYGKLELETQDFEKRFQPSK 360
Query: 361 DTEVDLVIKGPSVVNDISELGETTEHMDDSTISEQAEMMLLPSTNPLEAAEEIEKKQAFT 420
D+EV L I+ SVVNDISE ET E +D+ E+AE M L S+NPLE AEEIEKKQAFT
Sbjct: 361 DSEV-LAIEDLSVVNDISESRETAEQLDNFHTGERAETMSLSSSNPLELAEEIEKKQAFT 420
Query: 421 ALHWEEGVAAQPMKLEGIKGGSTTLGYFDIQADNSISRTISSHSFKREHGFPQVLAVHAN 480
ALHWEEGVAAQPM+LEGIKGG+T LGYFDIQADNSISRTISSHSFKREHGFPQ LAVHAN
Sbjct: 421 ALHWEEGVAAQPMRLEGIKGGTTALGYFDIQADNSISRTISSHSFKREHGFPQALAVHAN 480
Query: 481 YIAVGMSKGNIVVVASKYSAQNGDNMDAKMLLLGLQGDKSTAPVTSLCFNLQGDLLLAGY 540
YIAVGMSKGNIVVVASKYSAQNGDNMDAKMLLLG QGDKS APVTSLCFN QGDLLLAGY
Sbjct: 481 YIAVGMSKGNIVVVASKYSAQNGDNMDAKMLLLGSQGDKSNAPVTSLCFNQQGDLLLAGY 540
Query: 541 NDGHITVWDVLRASAAKIISGEHTSPVVHSLFLGQDAQVTRQFKAVTGDSKGLVLLHTFS 600
+DG +TVWDVLRA+AAK+ISGEHTSPVVHSLFLGQ+AQVTRQFKAVTGDSKGLVLLHTFS
Sbjct: 541 SDGQVTVWDVLRATAAKVISGEHTSPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFS 600
Query: 601 VVPLLNRFSIKTQCLLDGQKTGTVLSASALLLNESCGSSLPPTLANVAVSTSSIGSMMGG 660
VVPLLNRFSIKTQCLLDGQKTGTVLSASALLLNE CGSSLPP+L+NVAVSTSSIGSMMGG
Sbjct: 601 VVPLLNRFSIKTQCLLDGQKTGTVLSASALLLNEFCGSSLPPSLSNVAVSTSSIGSMMGG 660
Query: 661 VVGGDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSPTLEVYAQLSKPDGVREGSMPYT 720
VVGGDSGWKLFNEGSSLV EGVVIFATHQTALVVRLSPT+EVYA+LSKPDG++EGSMPYT
Sbjct: 661 VVGGDSGWKLFNEGSSLV-EGVVIFATHQTALVVRLSPTVEVYARLSKPDGIQEGSMPYT 720
Query: 721 AWKCSQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKAELKVCGKWSLESAAIGVAWLDD 780
AWKCSQS ETSPSEAVER+SLLAIAWDKMVQVAKLVK ELKVCGKWSLESAAIGVAWLDD
Sbjct: 721 AWKCSQSIETSPSEAVERISLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVAWLDD 780
Query: 781 QVLVVLTITGQLFLFEKDGTMIHQTSVFVDGFDKEDFIAYHTHFVNVLGNPEKAYHNCVA 840
QVLV+LT+TGQLFLFEKDGTMIHQTSVFVDGFDKEDFIA+HTHFVNVLGNPEKAYHNCVA
Sbjct: 781 QVLVILTVTGQLFLFEKDGTMIHQTSVFVDGFDKEDFIAHHTHFVNVLGNPEKAYHNCVA 840
Query: 841 VRGASIYVLGPMHLIISRLLPWKERVQVLRKAGDWMGALSMAITIYDGQAHGVIDLPRSL 900
VRGAS+YVLGP HL+ISRLLPWKERVQVLRKAGDWM ALSMAITIYDG AHGVIDLPRSL
Sbjct: 841 VRGASVYVLGPKHLVISRLLPWKERVQVLRKAGDWMTALSMAITIYDGHAHGVIDLPRSL 900
Query: 901 ESLQELVVPFLIELLLSYVDEVFSYISVAFCNQIEKNEKSVDVTSGGHSAHSEIKEQYNR 960
ESLQELV+PFLIELLLSYVDEVFSYISVAFCNQIEKNEK DVTSG HSAHSEIKEQYNR
Sbjct: 901 ESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDVTSGSHSAHSEIKEQYNR 960
Query: 961 VGGVAVEFCVHIRRTDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQALV 1020
VGGVAVEFCVHI RTDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQALV
Sbjct: 961 VGGVAVEFCVHITRTDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQALV 1020
Query: 1021 EHYSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL 1080
EHYS KGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL
Sbjct: 1021 EHYSQKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL 1080
Query: 1081 LAVLQTSKSERASAIGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQPLRDELLQFLLENSD 1140
LAVLQTSKS+RASAIGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQ LRDELLQFLLENSD
Sbjct: 1081 LAVLQTSKSKRASAIGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSD 1140
Query: 1141 SVDTRSISNKSSEIGYLKLYHLLELDTGATLDVLRCAFVEGEIPKTNSSPNGLDDVSMQV 1200
+VDT+SISNKSSE+GYL LYHLLELDTGATLDVLRCAFVEGEI K +SS +G D SMQV
Sbjct: 1141 TVDTKSISNKSSEVGYLNLYHLLELDTGATLDVLRCAFVEGEIVKADSSLDGSVDASMQV 1200
Query: 1201 QEEKNSTSGRKNVLIQSIVDALVHVLDQAICQTDRSAGGEDIALVGDWPSKKDLVHLFDF 1260
Q+EKNSTSGRKN L+Q++VDAL+H+LD+AI QT S GG++I LV DWPSKKDL HLFDF
Sbjct: 1201 QKEKNSTSGRKNFLVQNVVDALIHILDKAISQTYGSPGGDNITLVEDWPSKKDLFHLFDF 1260
Query: 1261 VANYVACGRATASKVVVGQILEHLISNSDIPETENDFLPKVTANSVHSRKREKQVLSLLE 1320
VANYVACG+ATASK VVGQILEHLISNSDIPE E D + VTANSVHSRKREKQVLSLLE
Sbjct: 1261 VANYVACGKATASKDVVGQILEHLISNSDIPEMEIDSVHSVTANSVHSRKREKQVLSLLE 1320
Query: 1321 VVPETHWNASSVLSMCEEAQFFQVCGLIHSIRHQYASALDSYMKDIDEPIHGFAFINRTL 1380
V+PETHWN SSVL MCE+AQFFQVCGLIHSI HQY+SALD YMKD++EPIH FAFINRTL
Sbjct: 1321 VIPETHWNPSSVLRMCEKAQFFQVCGLIHSISHQYSSALDGYMKDVEEPIHAFAFINRTL 1380
Query: 1381 LELNNYERTEFQAVVISRIPELFNLNREGTFFLVIDHFNNDASDILSQLRTHPRSLFLYL 1440
+EL+ E+TEF+ VVISRIPEL NLNREGTFFLVIDHF ND SDILSQL HPRSLFLYL
Sbjct: 1381 MELSYSEQTEFRGVVISRIPELLNLNREGTFFLVIDHFGNDVSDILSQLHNHPRSLFLYL 1440
Query: 1441 KTLIEVHLSGSLNFSCLRKDDIFGLNYSTKGLDDYLRKLSDFPKYLSNNPVDVTDDIIEL 1500
KTLIEVH SG+LNFSCL+KDD FG+NYSTKGLDDYL+KLSDFPK+LSNNPVDVTDDIIEL
Sbjct: 1441 KTLIEVHQSGNLNFSCLKKDDNFGVNYSTKGLDDYLQKLSDFPKFLSNNPVDVTDDIIEL 1500
Query: 1501 YVELLCQYERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS 1560
YVELLCQYERESVLKFLETFDSY VEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS
Sbjct: 1501 YVELLCQYERESVLKFLETFDSYHVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS 1560
Query: 1561 SLDKKFNDLEAAVESIVANGASSGSNDPRHFNSVLKLQEVNDIDVLLHACIGLCQRNTPR 1620
SLDKKF+DLEAAV IV NGASSGSND + F+SVLKLQEVNDI VLLHACIGLCQRNTPR
Sbjct: 1561 SLDKKFHDLEAAVGGIVTNGASSGSNDSQLFSSVLKLQEVNDIYVLLHACIGLCQRNTPR 1620
Query: 1621 LNSEESETLWFKLLDSFCEPLIDSYNYRTPAFGENQVQFLNESSGLQKDKEARIVTWRIL 1680
LNSEESETLWFKLLDSFCEPLI+S+NYRT +FGENQVQFLNESSG QKDKEA IVTWRIL
Sbjct: 1621 LNSEESETLWFKLLDSFCEPLIESFNYRTASFGENQVQFLNESSGSQKDKEAHIVTWRIL 1680
Query: 1681 KSDKAAHILKRLFSRFIKEIVEGMIGYVHLPIIMSRLLSDNGSQEFGDFKLTVLGMLGTF 1740
KS++ AHIL+ LFSRFI+EIVEGMIGYVHLP IMSRLLSDNGSQEFGDFKLT+LGMLGTF
Sbjct: 1681 KSNQTAHILRSLFSRFIREIVEGMIGYVHLPTIMSRLLSDNGSQEFGDFKLTILGMLGTF 1740
Query: 1741 GFERRILDTAKALIEDDTFYTMSLLKKGASHGYAPRNLVCCICNCLLIKSSSSYRVRVFN 1800
GFERRILDTAKALIEDDTFYTMSLLKKGASHGYAPR VCCICN LL+KSSSSYRVRVFN
Sbjct: 1741 GFERRILDTAKALIEDDTFYTMSLLKKGASHGYAPRGAVCCICNRLLVKSSSSYRVRVFN 1800
Query: 1801 CGHATHLQCEVLDNEASGGDFSCPVCIHSNQSQRSRGKALTEYSLVNTFSSGTQSLSGAS 1860
CGHATHLQCEVLDNEASG DFSCPVC+HSN SQRSRGKALTEYSLVN FSS TQS SGAS
Sbjct: 1801 CGHATHLQCEVLDNEASGADFSCPVCVHSNHSQRSRGKALTEYSLVNKFSSRTQSSSGAS 1860
Query: 1861 VQYPQETDLLELPYTLQQIPRFEILTNLQKNQRVIEIENVPQLRLAPPAVYHDKVTKGYH 1920
V YPQETDLLELPYTLQQIPRFEIL NLQKNQRVI+IEN+PQLRLAPPAVYHDKVTKGYH
Sbjct: 1861 VSYPQETDLLELPYTLQQIPRFEILANLQKNQRVIDIENMPQLRLAPPAVYHDKVTKGYH 1920
Query: 1921 LLVGESSSGI---EKQNKSKQLREVKVKRRSSLRFPLKASLFGE 1948
LLVG+SS GI EK NKS+QL+EVKVKR SSLRFPLKA+LFG+
Sbjct: 1921 LLVGDSSGGIEKVEKLNKSRQLKEVKVKRPSSLRFPLKANLFGK 1962
BLAST of MS010323 vs. NCBI nr
Match:
XP_023526489.1 (vacuolar protein sorting-associated protein 8 homolog isoform X1 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 3259.5 bits (8450), Expect = 0.0e+00
Identity = 1680/1964 (85.54%), Postives = 1783/1964 (90.78%), Query Frame = 0
Query: 1 MTKELTDTGVLPLMELDLNAFIHAHLSSSDDD---DDDDPSFPHRTIDEILNES----GS 60
MTKELTDT LP MELDLNAFIHAHLSS DDD D+DD SFPHR+IDEILNES S
Sbjct: 1 MTKELTDTETLPPMELDLNAFIHAHLSSGDDDHDHDEDDLSFPHRSIDEILNESSSSTSS 60
Query: 61 APSSPPHSPPYSGDRAVATRDGAVSESPSTPPSKSPFE-------APRSNQWNEKSVQLR 120
+PSSPP+SPP R++A RDG S S S PP KSPFE PRSNQ NEKSVQL+
Sbjct: 61 SPSSPPNSPPPRARRSIAARDGRASASRSIPPFKSPFEEIIKASKVPRSNQRNEKSVQLK 120
Query: 121 PAAVSLVKVGELTDDLFRRGSRPLPSLFGGVRSNAKPGAALAAAAAASRSMPAPHAAAIK 180
P +VS KVGELTDD FRRGSR LPSLFGGVRSNAKPGAALAAAAAASRSMPAPHAAAIK
Sbjct: 121 PGSVSHTKVGELTDDPFRRGSRALPSLFGGVRSNAKPGAALAAAAAASRSMPAPHAAAIK 180
Query: 181 SRRAGHGSMVIDDDELASPSADDSEAVFGGLYSTNTHSEESGENSTVMDDTDADYQVASI 240
SRR+GHGS+V+DDDELAS SA DSE VF LYST HS+ES E S + + +ADYQ AS+
Sbjct: 181 SRRSGHGSVVLDDDELASSSAVDSEFVFDDLYSTIDHSKESREKSISLVERNADYQGASV 240
Query: 241 NVGGGVWARDRIRDDVPDNDEFRITKGLKCVAEQRSVDDVNFDESTTILPPVEANDGSVS 300
NVG WARD IRD V NDEFRITK +C AEQ VDDVNFDES+T LPPVEAN S+S
Sbjct: 241 NVGVEFWARDNIRDCVLYNDEFRITKDTECEAEQSFVDDVNFDESSTTLPPVEANGRSLS 300
Query: 301 DYADKNVCSTELRPSILDVDESNEGAIPSSSEPDNDGSAVDYGSLEWETQDFKKCLQQSK 360
D AD NVCS + P++LD DESNEGA P S +PD D SAV YG LE ETQDF+K Q SK
Sbjct: 301 DSADNNVCSMDAEPTVLDGDESNEGAFPCSPKPDYDRSAVGYGRLELETQDFEKRSQPSK 360
Query: 361 DTEVDLVIKGPSVVNDISELGETTEHMDDSTISEQAEMMLLPSTNPLEAAEEIEKKQAFT 420
D+EV L I+ SVVNDISE ET + +D+ E+AE M L S+NPLE AEEIEKKQAFT
Sbjct: 361 DSEV-LAIEDLSVVNDISESRETAKQLDNFHTGERAETMSLSSSNPLELAEEIEKKQAFT 420
Query: 421 ALHWEEGVAAQPMKLEGIKGGSTTLGYFDIQADNSISRTISSHSFKREHGFPQVLAVHAN 480
ALHWEEGVAAQPM+LEGIKGG+T LGYFDIQADNSISRTISSHSFKREHGFPQ LAVHAN
Sbjct: 421 ALHWEEGVAAQPMRLEGIKGGTTALGYFDIQADNSISRTISSHSFKREHGFPQALAVHAN 480
Query: 481 YIAVGMSKGNIVVVASKYSAQNGDNMDAKMLLLGLQGDKSTAPVTSLCFNLQGDLLLAGY 540
YIAVGMSKGNIVVVASKYSAQNGDNMDAKMLLLG QGDKSTAPVTSLCFN QGDLLLAGY
Sbjct: 481 YIAVGMSKGNIVVVASKYSAQNGDNMDAKMLLLGSQGDKSTAPVTSLCFNQQGDLLLAGY 540
Query: 541 NDGHITVWDVLRASAAKIISGEHTSPVVHSLFLGQDAQVTRQFKAVTGDSKGLVLLHTFS 600
+DG +TVWDVLRA+AAK+ISGEHTSPVVHSLFLGQ+AQVTRQFKAVTGDSKGLVLLHTFS
Sbjct: 541 SDGQVTVWDVLRATAAKVISGEHTSPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFS 600
Query: 601 VVPLLNRFSIKTQCLLDGQKTGTVLSASALLLNESCGSSLPPTLANVAVSTSSIGSMMGG 660
VVPLLNRFSIKTQCLLDGQKTGTVLSASALLLNE CGSSLPP+L+NVAVSTSSIGSMMGG
Sbjct: 601 VVPLLNRFSIKTQCLLDGQKTGTVLSASALLLNEFCGSSLPPSLSNVAVSTSSIGSMMGG 660
Query: 661 VVGGDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSPTLEVYAQLSKPDGVREGSMPYT 720
VVGGDSGWKLFNEGSSLV EGVVIFATHQTALVVRLSPT+EVYA+LSKPDG++EGSMPYT
Sbjct: 661 VVGGDSGWKLFNEGSSLV-EGVVIFATHQTALVVRLSPTVEVYARLSKPDGIQEGSMPYT 720
Query: 721 AWKCSQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKAELKVCGKWSLESAAIGVAWLDD 780
AWKCSQS ETSPSEAVER+SLLAIAWDKMVQVAKLVK EL VCGKWSLESAAIGVAWLDD
Sbjct: 721 AWKCSQSIETSPSEAVERISLLAIAWDKMVQVAKLVKTELNVCGKWSLESAAIGVAWLDD 780
Query: 781 QVLVVLTITGQLFLFEKDGTMIHQTSVFVDGFDKEDFIAYHTHFVNVLGNPEKAYHNCVA 840
QVLV+LT+TGQLFLFEKDGTMIHQTSVFVDGFDKEDFIA+HTHFVNVLGNPEKAYHNCVA
Sbjct: 781 QVLVILTVTGQLFLFEKDGTMIHQTSVFVDGFDKEDFIAHHTHFVNVLGNPEKAYHNCVA 840
Query: 841 VRGASIYVLGPMHLIISRLLPWKERVQVLRKAGDWMGALSMAITIYDGQAHGVIDLPRSL 900
VRGAS+YVLGP HL+ISRLLPWKERVQVLRKAGDWM ALSMAITIYDG AHGVIDLPRSL
Sbjct: 841 VRGASVYVLGPKHLVISRLLPWKERVQVLRKAGDWMTALSMAITIYDGHAHGVIDLPRSL 900
Query: 901 ESLQELVVPFLIELLLSYVDEVFSYISVAFCNQIEKNEKSVDVTSGGHSAHSEIKEQYNR 960
ESLQELV+PFLIELLLSYVDEVFSYISVAFCNQIEKNEK DVTSG HSAHSEIKEQYNR
Sbjct: 901 ESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDVTSGSHSAHSEIKEQYNR 960
Query: 961 VGGVAVEFCVHIRRTDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQALV 1020
VGGVAVEFCVHI RTDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQALV
Sbjct: 961 VGGVAVEFCVHITRTDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQALV 1020
Query: 1021 EHYSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL 1080
EHYS KGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL
Sbjct: 1021 EHYSQKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL 1080
Query: 1081 LAVLQTSKSERASAIGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQPLRDELLQFLLENSD 1140
LAVLQTSKS+RASAIGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQ LRDELLQFLLENSD
Sbjct: 1081 LAVLQTSKSKRASAIGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSD 1140
Query: 1141 SVDTRSISNKSSEIGYLKLYHLLELDTGATLDVLRCAFVEGEIPKTNSSPNGLDDVSMQV 1200
+VDTRSISNKSSE+GYL LYHLLELDTGATLDVLRCAFVEGEI K +SS +G D SMQV
Sbjct: 1141 TVDTRSISNKSSEVGYLNLYHLLELDTGATLDVLRCAFVEGEIVKADSSLDGSVDASMQV 1200
Query: 1201 QEEKNSTSGRKNVLIQSIVDALVHVLDQAICQTDRSAGGEDIALVGDWPSKKDLVHLFDF 1260
Q+EKNSTSGRKN L+Q++VDALVH+LD+AI QT S GG++I LV DWPSKKDL HLFDF
Sbjct: 1201 QKEKNSTSGRKNFLVQNVVDALVHILDKAISQTYGSPGGDNITLVEDWPSKKDLFHLFDF 1260
Query: 1261 VANYVACGRATASKVVVGQILEHLISNSDIPETENDFLPKVTANSVHSRKREKQVLSLLE 1320
VANYVACG+ATASK VVGQILEHLISNSDIPE E DF+ VTANSVHSRKREKQVLSLLE
Sbjct: 1261 VANYVACGKATASKDVVGQILEHLISNSDIPEMEIDFVHSVTANSVHSRKREKQVLSLLE 1320
Query: 1321 VVPETHWNASSVLSMCEEAQFFQVCGLIHSIRHQYASALDSYMKDIDEPIHGFAFINRTL 1380
V+PETHWN SSVL MCE+AQFFQVCGLIHSI HQY+SALD YMKD++EPIH FAFINRTL
Sbjct: 1321 VIPETHWNPSSVLRMCEKAQFFQVCGLIHSISHQYSSALDGYMKDVEEPIHAFAFINRTL 1380
Query: 1381 LELNNYERTEFQAVVISRIPELFNLNREGTFFLVIDHFNNDASDILSQLRTHPRSLFLYL 1440
+EL+N E+TEF+ VVISRIPEL NLNREGTFFLVIDHF ND SDILS+L HPRSLFLYL
Sbjct: 1381 MELSNSEQTEFRGVVISRIPELLNLNREGTFFLVIDHFGNDVSDILSRLHNHPRSLFLYL 1440
Query: 1441 KTLIEVHLSGSLNFSCLRKDDIFGLNYSTKGLDDYLRKLSDFPKYLSNNPVDVTDDIIEL 1500
KTLIEVH SG+LNFSCL+KDD FG+NYSTKGLDDYL+KLSDFPK+LSNNPVDVTDDIIEL
Sbjct: 1441 KTLIEVHQSGNLNFSCLKKDDNFGVNYSTKGLDDYLQKLSDFPKFLSNNPVDVTDDIIEL 1500
Query: 1501 YVELLCQYERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS 1560
YVELLC+YERESVLKFLETFDSY VEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS
Sbjct: 1501 YVELLCRYERESVLKFLETFDSYHVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS 1560
Query: 1561 SLDKKFNDLEAAVESIVANGASSGSNDPRHFNSVLKLQEVNDIDVLLHACIGLCQRNTPR 1620
SLDKKF+DLEAAV IV NGASSGSND + F+SVLKLQEVNDI VLLHACIGLCQRNTPR
Sbjct: 1561 SLDKKFHDLEAAVGGIVTNGASSGSNDSQLFSSVLKLQEVNDIYVLLHACIGLCQRNTPR 1620
Query: 1621 LNSEESETLWFKLLDSFCEPLIDSYNYRTPAFGENQVQFLNESSGLQKDKEARIVTWRIL 1680
LNSEESETLWFKLLDSFCEPLI+S+NYRT +FGENQVQFLNESSG QKDKEA IVTWRIL
Sbjct: 1621 LNSEESETLWFKLLDSFCEPLIESFNYRTASFGENQVQFLNESSGSQKDKEAHIVTWRIL 1680
Query: 1681 KSDKAAHILKRLFSRFIKEIVEGMIGYVHLPIIMSRLLSDNGSQEFGDFKLTVLGMLGTF 1740
KS++ AHIL+ LFSRFI+EIVEGMIGYVHLP IMSRLLSDNGSQEFGDFKLT+LGMLGTF
Sbjct: 1681 KSNQTAHILRSLFSRFIREIVEGMIGYVHLPTIMSRLLSDNGSQEFGDFKLTILGMLGTF 1740
Query: 1741 GFERRILDTAKALIEDDTFYTMSLLKKGASHGYAPRNLVCCICNCLLIKSSSSYRVRVFN 1800
GFERRILDTAKALIEDDTFYTMSLLKKGASHGYAPR VCCICN LL+KSSSSYRVRVFN
Sbjct: 1741 GFERRILDTAKALIEDDTFYTMSLLKKGASHGYAPRGAVCCICNRLLVKSSSSYRVRVFN 1800
Query: 1801 CGHATHLQCEVLDNEASGGDFSCPVCIHSNQSQRSRGKALTEYSLVNTFSSGTQSLSGAS 1860
CGHATHLQCEVLDNEASGGDFSCPVC+HSN SQRSRGKALTEYSLVN FSS TQS SGAS
Sbjct: 1801 CGHATHLQCEVLDNEASGGDFSCPVCVHSNHSQRSRGKALTEYSLVNKFSSRTQSSSGAS 1860
Query: 1861 VQYPQETDLLELPYTLQQIPRFEILTNLQKNQRVIEIENVPQLRLAPPAVYHDKVTKGYH 1920
V YPQETDLLELPYTLQQIPRFEIL NLQKNQRVI+IEN+PQLRLAPPAVYHDKVTKGYH
Sbjct: 1861 VSYPQETDLLELPYTLQQIPRFEILANLQKNQRVIDIENMPQLRLAPPAVYHDKVTKGYH 1920
Query: 1921 LLVGESSSG---IEKQNKSKQLREVKVKRRSSLRFPLKASLFGE 1948
LLVG+SS G +EK NKS+QL+EVKVKR SSLRFPLKA+LFG+
Sbjct: 1921 LLVGDSSGGVEKVEKLNKSRQLKEVKVKRPSSLRFPLKANLFGK 1962
BLAST of MS010323 vs. NCBI nr
Match:
XP_022924308.1 (vacuolar protein sorting-associated protein 8 homolog isoform X1 [Cucurbita moschata])
HSP 1 Score: 3247.2 bits (8418), Expect = 0.0e+00
Identity = 1676/1964 (85.34%), Postives = 1776/1964 (90.43%), Query Frame = 0
Query: 1 MTKELTDTGVLPLMELDLNAFIHAHLSSSDDDDDDDP---SFPHRTIDEILNES----GS 60
MTKELTDT LP MELDLNAFIHAHLSS DDDDDDD SFPHR+IDEILNES S
Sbjct: 1 MTKELTDTETLPPMELDLNAFIHAHLSSGDDDDDDDEDDLSFPHRSIDEILNESSSSTSS 60
Query: 61 APSSPPHSPPYSGDRAVATRDGAVSESPSTPPSKSPFE-------APRSNQWNEKSVQLR 120
+PSSPP+SPP R++A RDG S S S PP KSPFE PRSNQ NEKSVQL+
Sbjct: 61 SPSSPPNSPPPRDHRSIAARDGRASASRSIPPFKSPFEEIIKASKVPRSNQRNEKSVQLK 120
Query: 121 PAAVSLVKVGELTDDLFRRGSRPLPSLFGGVRSNAKPGAALAAAAAASRSMPAPHAAAIK 180
P +VS KVGELTDD FRRGSR LPSLFGGVRSNAKPGAALAAAAAASRSMPAPHAAAIK
Sbjct: 121 PGSVSHTKVGELTDDPFRRGSRALPSLFGGVRSNAKPGAALAAAAAASRSMPAPHAAAIK 180
Query: 181 SRRAGHGSMVIDDDELASPSADDSEAVFGGLYSTNTHSEESGENSTVMDDTDADYQVASI 240
SRR+GHGS+V+DDDELAS SA DSE VF LY T HS+ES E S + + +ADYQ AS+
Sbjct: 181 SRRSGHGSVVLDDDELASSSAVDSEFVFDDLYPTIDHSKESREKSISLVERNADYQGASV 240
Query: 241 NVGGGVWARDRIRDDVPDNDEFRITKGLKCVAEQRSVDDVNFDESTTILPPVEANDGSVS 300
NVGG WARD IRD V NDEFRITK +C AEQ VDDVNFDES+T LPPVEAN S+S
Sbjct: 241 NVGGEFWARDNIRDCVLYNDEFRITKDTECEAEQSFVDDVNFDESSTTLPPVEANGRSLS 300
Query: 301 DYADKNVCSTELRPSILDVDESNEGAIPSSSEPDNDGSAVDYGSLEWETQDFKKCLQQSK 360
D AD NVCS + P++LDVDESNEGA PSS +PD D SAV YG LE ETQDF+K Q SK
Sbjct: 301 DSADNNVCSMDAEPTVLDVDESNEGAFPSSPKPDYDRSAVGYGRLELETQDFEKRFQPSK 360
Query: 361 DTEVDLVIKGPSVVNDISELGETTEHMDDSTISEQAEMMLLPSTNPLEAAEEIEKKQAFT 420
D+EV L I+ SVVNDISE ET E +D+ E+AE M L S+NPLE AEEIEKKQAFT
Sbjct: 361 DSEV-LAIEDLSVVNDISESRETAEQLDNFHTGERAETMSLSSSNPLELAEEIEKKQAFT 420
Query: 421 ALHWEEGVAAQPMKLEGIKGGSTTLGYFDIQADNSISRTISSHSFKREHGFPQVLAVHAN 480
ALHWEEGVAAQPM+LEGIKGG+T LGYFDIQADNSISRTISSHSFKREHGFPQ LAVHAN
Sbjct: 421 ALHWEEGVAAQPMRLEGIKGGTTALGYFDIQADNSISRTISSHSFKREHGFPQALAVHAN 480
Query: 481 YIAVGMSKGNIVVVASKYSAQNGDNMDAKMLLLGLQGDKSTAPVTSLCFNLQGDLLLAGY 540
YIAVGMSKGNIVVVASKYSAQNGDNMDAKMLLLG QGDKSTAPVTSLCFN QGDLLLAGY
Sbjct: 481 YIAVGMSKGNIVVVASKYSAQNGDNMDAKMLLLGSQGDKSTAPVTSLCFNQQGDLLLAGY 540
Query: 541 NDGHITVWDVLRASAAKIISGEHTSPVVHSLFLGQDAQVTRQFKAVTGDSKGLVLLHTFS 600
+DG +TVWDVLRA+AAK+ISGEHTSPVVHSLFLGQ+AQVTRQFKAVTGDSKGLVLLHTFS
Sbjct: 541 SDGQVTVWDVLRATAAKVISGEHTSPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFS 600
Query: 601 VVPLLNRFSIKTQCLLDGQKTGTVLSASALLLNESCGSSLPPTLANVAVSTSSIGSMMGG 660
VVPLLNRFSIKTQCLLDGQKTGTVLSASALLLNE CGSSLPP+L+NVAVSTSSIGSMMGG
Sbjct: 601 VVPLLNRFSIKTQCLLDGQKTGTVLSASALLLNEFCGSSLPPSLSNVAVSTSSIGSMMGG 660
Query: 661 VVGGDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSPTLEVYAQLSKPDGVREGSMPYT 720
VVGGDSGWKLFNEGSSLV EGVVIFATHQTALVVRLSPT+EVYA+LSKPDG++EGSMPYT
Sbjct: 661 VVGGDSGWKLFNEGSSLV-EGVVIFATHQTALVVRLSPTVEVYARLSKPDGIQEGSMPYT 720
Query: 721 AWKCSQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKAELKVCGKWSLESAAIGVAWLDD 780
AWKCSQS ETSPSEAVER+SLLAIAWDKMVQVAKLVK ELKVCGKWSLESAAIGVAWLDD
Sbjct: 721 AWKCSQSIETSPSEAVERISLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVAWLDD 780
Query: 781 QVLVVLTITGQLFLFEKDGTMIHQTSVFVDGFDKEDFIAYHTHFVNVLGNPEKAYHNCVA 840
QVLV+LT+TGQLFLFEKDGTMIHQTSVFVDGFDKEDFIA+HTHFVNVLGNPEKAYHNCVA
Sbjct: 781 QVLVILTVTGQLFLFEKDGTMIHQTSVFVDGFDKEDFIAHHTHFVNVLGNPEKAYHNCVA 840
Query: 841 VRGASIYVLGPMHLIISRLLPWKERVQVLRKAGDWMGALSMAITIYDGQAHGVIDLPRSL 900
VRGAS+YVLGP HL+ISRLLPWKERVQVLRKAGDWM ALSMAITIYDG AHGVIDLPRSL
Sbjct: 841 VRGASVYVLGPKHLVISRLLPWKERVQVLRKAGDWMTALSMAITIYDGHAHGVIDLPRSL 900
Query: 901 ESLQELVVPFLIELLLSYVDEVFSYISVAFCNQIEKNEKSVDVTSGGHSAHSEIKEQYNR 960
ESLQELV+PFLIELLLSYVDEVFSYIS+AFCNQIEKNEK DVTSG HSAHSEIKEQYNR
Sbjct: 901 ESLQELVMPFLIELLLSYVDEVFSYISLAFCNQIEKNEKLDDVTSGSHSAHSEIKEQYNR 960
Query: 961 VGGVAVEFCVHIRRTDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQALV 1020
VGGVAVEFCVHI RTDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQALV
Sbjct: 961 VGGVAVEFCVHITRTDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQALV 1020
Query: 1021 EHYSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL 1080
EHYS KGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL
Sbjct: 1021 EHYSQKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL 1080
Query: 1081 LAVLQTSKSERASAIGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQPLRDELLQFLLENSD 1140
LAVLQTSKS+RASAIGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQ LRDELLQFLLENSD
Sbjct: 1081 LAVLQTSKSKRASAIGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSD 1140
Query: 1141 SVDTRSISNKSSEIGYLKLYHLLELDTGATLDVLRCAFVEGEIPKTNSSPNGLDDVSMQV 1200
+VDTRSISNKSSE+GYL LYHLLELDTGATLDVLRCAFVEGEI K +SS +G D SMQV
Sbjct: 1141 TVDTRSISNKSSEVGYLNLYHLLELDTGATLDVLRCAFVEGEIVKADSSLDGSVDASMQV 1200
Query: 1201 QEEKNSTSGRKNVLIQSIVDALVHVLDQAICQTDRSAGGEDIALVGDWPSKKDLVHLFDF 1260
Q+E KN L+Q++VDAL+H+LD+AI QT S GG++I LV DWPSKKDL HLFDF
Sbjct: 1201 QKE-------KNFLVQNVVDALIHILDKAISQTYGSPGGDNITLVEDWPSKKDLFHLFDF 1260
Query: 1261 VANYVACGRATASKVVVGQILEHLISNSDIPETENDFLPKVTANSVHSRKREKQVLSLLE 1320
VANYVACG+AT SK VVGQILEHLISNSDIPE E DF+ VTANSVHSRKREKQVLSLLE
Sbjct: 1261 VANYVACGKATVSKDVVGQILEHLISNSDIPEMEIDFVHSVTANSVHSRKREKQVLSLLE 1320
Query: 1321 VVPETHWNASSVLSMCEEAQFFQVCGLIHSIRHQYASALDSYMKDIDEPIHGFAFINRTL 1380
V+PETHWN SSVL MCE+AQFFQVCGLIHSI HQY+SALD YMKD++EPIH FAFINRTL
Sbjct: 1321 VIPETHWNPSSVLRMCEKAQFFQVCGLIHSISHQYSSALDGYMKDVEEPIHAFAFINRTL 1380
Query: 1381 LELNNYERTEFQAVVISRIPELFNLNREGTFFLVIDHFNNDASDILSQLRTHPRSLFLYL 1440
+EL+ E+TEF+ VVISRIPEL NLNREGTFFLVIDHF ND SDILSQL HPRSLFLYL
Sbjct: 1381 MELSYSEQTEFRGVVISRIPELLNLNREGTFFLVIDHFGNDVSDILSQLHNHPRSLFLYL 1440
Query: 1441 KTLIEVHLSGSLNFSCLRKDDIFGLNYSTKGLDDYLRKLSDFPKYLSNNPVDVTDDIIEL 1500
KTLIEVH SG+LNFSCL+KDD FG+NYSTKGLDDYL+KLSDFPK+LSNNPVDVTDDIIEL
Sbjct: 1441 KTLIEVHQSGNLNFSCLKKDDNFGVNYSTKGLDDYLQKLSDFPKFLSNNPVDVTDDIIEL 1500
Query: 1501 YVELLCQYERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS 1560
YVELLCQYERESVLKFLETFDSY VEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS
Sbjct: 1501 YVELLCQYERESVLKFLETFDSYHVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS 1560
Query: 1561 SLDKKFNDLEAAVESIVANGASSGSNDPRHFNSVLKLQEVNDIDVLLHACIGLCQRNTPR 1620
SLDKKF+DLEAAV IV NGASSGSND + F+SVLKLQEVNDI VLLHACIGLCQRNTPR
Sbjct: 1561 SLDKKFHDLEAAVGGIVTNGASSGSNDSQLFSSVLKLQEVNDIYVLLHACIGLCQRNTPR 1620
Query: 1621 LNSEESETLWFKLLDSFCEPLIDSYNYRTPAFGENQVQFLNESSGLQKDKEARIVTWRIL 1680
LNSEESETLWFKLLDSFCEPLI+S+NYRT +FGENQVQFLNESSG QKDKEA IVTWRIL
Sbjct: 1621 LNSEESETLWFKLLDSFCEPLIESFNYRTASFGENQVQFLNESSGSQKDKEAHIVTWRIL 1680
Query: 1681 KSDKAAHILKRLFSRFIKEIVEGMIGYVHLPIIMSRLLSDNGSQEFGDFKLTVLGMLGTF 1740
KS++ AHIL+ LFSRFI+EIVEGMIGYVHLP IMSRLLSDNGSQEFGDFKLT+LGMLGTF
Sbjct: 1681 KSNQTAHILRSLFSRFIREIVEGMIGYVHLPTIMSRLLSDNGSQEFGDFKLTILGMLGTF 1740
Query: 1741 GFERRILDTAKALIEDDTFYTMSLLKKGASHGYAPRNLVCCICNCLLIKSSSSYRVRVFN 1800
GFERRILDTAKALIEDDTFYTMSLLKKGASHGYAPR VCCICN LL+KSSSSYRVRVFN
Sbjct: 1741 GFERRILDTAKALIEDDTFYTMSLLKKGASHGYAPRGAVCCICNRLLVKSSSSYRVRVFN 1800
Query: 1801 CGHATHLQCEVLDNEASGGDFSCPVCIHSNQSQRSRGKALTEYSLVNTFSSGTQSLSGAS 1860
CGHATHLQCEVLDNEASGGDFSCPVC+HSN SQRSRGKALTEYSLVN FSS TQS SGA
Sbjct: 1801 CGHATHLQCEVLDNEASGGDFSCPVCVHSNHSQRSRGKALTEYSLVNKFSSRTQSSSGAF 1860
Query: 1861 VQYPQETDLLELPYTLQQIPRFEILTNLQKNQRVIEIENVPQLRLAPPAVYHDKVTKGYH 1920
V YPQETDLLELPYTLQQIPRFEIL NLQKNQRVI+IEN+PQLRLAPPAVYHDKVTKGYH
Sbjct: 1861 VSYPQETDLLELPYTLQQIPRFEILANLQKNQRVIDIENMPQLRLAPPAVYHDKVTKGYH 1920
Query: 1921 LLVGESSSGI---EKQNKSKQLREVKVKRRSSLRFPLKASLFGE 1948
LLVG+SS GI EK NKS+QL+EVKVKR SSLRFPLKA+LFG+
Sbjct: 1921 LLVGDSSGGIEKVEKLNKSRQLKEVKVKRPSSLRFPLKANLFGK 1955
BLAST of MS010323 vs. ExPASy Swiss-Prot
Match:
Q8N3P4 (Vacuolar protein sorting-associated protein 8 homolog OS=Homo sapiens OX=9606 GN=VPS8 PE=1 SV=3)
HSP 1 Score: 331.3 bits (848), Expect = 7.6e-89
Identity = 335/1416 (23.66%), Postives = 593/1416 (41.88%), Query Frame = 0
Query: 442 ISRTISSHSFKREHGFPQVLAVHANYIAVGMSKGNIVVVASKYSAQNGDNMDAKMLLLGL 501
IS I S + K + G P +AV ++ IAVG S G ++ D A L LG
Sbjct: 141 ISAQIVSAADKVDAGLPTAIAV-SSLIAVGTSHGLALIFGK-------DQNQALRLCLGS 200
Query: 502 QG-DKSTAPVTSLCFNLQGDLLLAGYNDGHITVWDVLRASAAKIISGEHT--SPVVHSLF 561
+++L N LL G+ G IT+WD+ + I+ H + ++H F
Sbjct: 201 TSVGGQYGAISALSINNDCSRLLCGFAKGQITMWDLASGKLLRSITDAHPPGTAILHIKF 260
Query: 562 LGQDAQVTRQFKAVTGDSKGLVLLHTFSVVPLLNRFSIKTQCLLDGQKTGTVLSASALLL 621
A+ DS G V TF V + + +++CL G K G V L
Sbjct: 261 TDDPT------LAICNDSGGSVFELTFKRV--MGVRTCESRCLFSGSK-GEVCCIEPL-- 320
Query: 622 NESCGSSLPPTLANVAVSTSSIGSMMGGVVGGDSGWKLFNEGSSLVEEGVVIFATHQTAL 681
P L + ++ S+ +M A+ L
Sbjct: 321 ------HSKPELKDHPITQFSLLAM----------------------------ASLTKIL 380
Query: 682 VVRLSPTLEVYAQLSKPDG-VREGSMPYTAWK--CSQSFETSPSEAVER---VSLLAIAW 741
V+ L P+L+V+ ++ P G + S+P AW Q++ +P A R V L +
Sbjct: 381 VIGLKPSLKVW--MTFPYGRMDPSSVPLLAWHFVAVQNY-VNPMLAFCRGDVVHFLLVKR 440
Query: 742 DKMVQVAKLVKAELKVCGKWSLESAAIGVAWLDDQVLVVLTITGQLFLFEKDGTMIHQTS 801
D+ + + L L I W++ + +V+L +L + ++ Q
Sbjct: 441 DESGAIHVTKQKHL------HLYYDLINFTWINSRTVVLLDSVEKLHVIDRQ----TQEE 500
Query: 802 VFVDGFDKEDFIAYHTHFVNVL--GN--------PEKAYHNCVAVRGASIYVLGPMHLII 861
+ + + +HF ++ GN EKA + ++ G I+ LG + +
Sbjct: 501 LETVEISEVQLVYNSSHFKSLATGGNVSQALALVGEKACYQSISSYGGQIFYLGTKSVYV 560
Query: 862 SRLLPWKERVQVLRKAGDWMGALSMAITIYDGQAHGVIDLPRSLESLQELVVPFLIELLL 921
L W+ERV L K AL++A + ++G+A V+ L + +V ++E+L
Sbjct: 561 MMLRSWRERVDHLLKQDCLTEALALAWSFHEGKAKAVVGLSGDASKRKAIVADRMVEILF 620
Query: 922 SYVDEVFSYISVAFCNQIEKNEKSVDVTSGGHSAHSEIKEQYNRVGGVAVEFCVHIRRTD 981
Y D ++ + V +++ + + V V++C+ ++R D
Sbjct: 621 HYADRALKKC---------PDQGKIQV----------MEQHFQDMVPVIVDYCLLLQRKD 680
Query: 982 ILFDEIFSKFVAVQ-QRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKGWLQRVEQC 1041
+LF +++ K + FLE LEPYIL D L + P++M+ L+ H+ K ++ VE
Sbjct: 681 LLFSQMYDKLSENSVAKGVFLECLEPYILSDKLVGITPQVMKDLIVHFQDKKLMENVEAL 740
Query: 1042 VLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLQ------TSKS 1101
++HMDI+SLD QVV +C ++ LY A++Y++N+G+++F +P+E+L V+ + +
Sbjct: 741 IVHMDITSLDIQQVVLMCWENRLYDAMIYVYNRGMNEFISPMEKLFRVIAPPLNAGKTLT 800
Query: 1102 ERASAIGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQPLRDELLQFLLENSDSVDTRSISN 1161
+ +G K LVY+ C +G A+P G + V +++++ +FL+ S
Sbjct: 801 DEQVVMGNKLLVYISCCLAGRAYP--LGDIPEDLVPLVKNQVFEFLIR------LHSAEA 860
Query: 1162 KSSEIGYLKLYHLLELDTGATLDVLRCAFVEGEIPKTNSSPNGLDDVSMQVQEEKNSTSG 1221
E Y + LL DT L+VL F + + N V Q
Sbjct: 861 SPEEEIYPYIRTLLHFDTREFLNVLALTFEDFK--------NDKQAVEYQ---------- 920
Query: 1222 RKNVLIQSIVDALVHVLDQAICQTDRSAGGEDIALVGDWPSKKDLVHLFDFVANYVACGR 1281
Q IVD L+ V+ + T G LF F+A +A
Sbjct: 921 ------QRIVDILLKVMVENSDFTPSQVG-----------------CLFTFLARQLAKPD 980
Query: 1282 AT--ASKVVVGQILEHLISNSDIPETENDFLPKVTANSVHSRKREKQVLSLLEVVPETHW 1341
T ++ + Q+LE L S D +S HS +R++ +L LL+ +
Sbjct: 981 NTLFVNRTLFDQVLEFLCSPDD--------------DSRHS-ERQQVLLELLQAGGIVQF 1040
Query: 1342 NASSVLSMCEEAQFFQVCGLIHSIRHQYASALDSYMKDIDEPIHGFAFINRTLLELNNYE 1401
S ++ M E+A+F+Q+C ++ HQY +D Y++D F +I+ +L + +
Sbjct: 1041 EESRLIRMAEKAEFYQICEFMYEREHQYDKIIDCYLRDPLREEEVFNYIH-NILSIPGHS 1100
Query: 1402 RTEFQAV---VISRIPELFNLNREGTFFLVIDHFNNDASDILSQLRTHPRSLFLYLKTLI 1461
E Q+V + I EL +L LV HF+ ++ +L+ LF +L++L+
Sbjct: 1101 AEEKQSVWQKAMDHIEELVSLKPCKAAELVATHFSGHIETVIKKLQNQV-LLFKFLRSLL 1160
Query: 1462 EVHLSGSLNFSCLRKDDIFGLNYSTKGLDDYLRKLSDFPKYLSNNPVDVTDDIIELYVEL 1521
+ +G+ +++ + ++ I E ++EL
Sbjct: 1161 D----------------------PREGI------------HVNQELLQISPCITEQFIEL 1220
Query: 1522 LCQYERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDK 1581
LCQ+ V++ L+ + YR+E +++ Q+Y++ + A+LLE+ GD+ A + L L
Sbjct: 1221 LCQFNPTQVIETLQVLECYRLEETIQITQKYQLHEVTAYLLEKKGDIHGAFLIMLERLQS 1280
Query: 1582 KFNDLEAAVESIVANGASSGSNDPRHFNSVLKLQEVNDIDVLLHACIGLCQRNTPRLNSE 1641
K + V + + DP + D++ + I LCQRN+ LN +
Sbjct: 1281 KLQE--------VTHQGENTKEDP----------SLKDVEDTMVETIALCQRNSHNLNQQ 1314
Query: 1642 ESETLWFKLLDSFCEPLIDSYNYRTPAFGENQVQFLNESSGLQKDKEARIVTWRILKSDK 1701
+ E LWF LL++ P QK L S
Sbjct: 1341 QREALWFPLLEAMMAP--------------------------QK-----------LSSSA 1314
Query: 1702 AAHILKRLFSRFIKEIVEGMIGYVHLPIIMSRLLSD--NGSQEFGDFKLTVLGMLGTFGF 1761
H+ +++ M ++ LP I+ R+L D G + G+ + +LGML TF +
Sbjct: 1401 IPHLHSEALKSLTMQVLNSMAAFIALPSILQRILQDPVYGKGKLGEIQGLILGMLDTFNY 1314
Query: 1762 ERRILDTAKALIEDDTFYTMSLLKKGASHGYAPRNLVCCIC-NCLLIKSSSSYRVRVFNC 1818
E+ +L+T +L+ D +++ L+ + G P+ C IC + + + VF+C
Sbjct: 1461 EQTLLETTTSLLNQDLHWSLCNLRASVTRGLNPKQDYCSICLQQYKRRQEMADEIIVFSC 1314
BLAST of MS010323 vs. ExPASy Swiss-Prot
Match:
Q0P5W1 (Vacuolar protein sorting-associated protein 8 homolog OS=Mus musculus OX=10090 GN=Vps8 PE=1 SV=1)
HSP 1 Score: 328.9 bits (842), Expect = 3.8e-88
Identity = 362/1528 (23.69%), Postives = 628/1528 (41.10%), Query Frame = 0
Query: 442 ISRTISSHSFKREHGFPQVLAVHANYIAVGMSKGNIVVVASKYSAQNGDNMDAKMLLLGL 501
IS I S + K + G P +AV ++ IAVG S G ++ D A L LG
Sbjct: 141 ISAQIVSAADKVDAGLPTAIAV-SSLIAVGTSHGLALIF---------DQNQALRLCLGS 200
Query: 502 QG-DKSTAPVTSLCFNLQGDLLLAGYNDGHITVWDVLRASAAKIISGEHT--SPVVHSLF 561
+++L N LL G+ G IT+WD+ + I+ H + ++H F
Sbjct: 201 TSVGGQYGAISALSINNDCSRLLCGFAKGQITMWDLASGKLLRSITDAHPPGTAILHIKF 260
Query: 562 LGQDAQVTRQFKAVTGDSKGLVLLHTFSVVPLLNRFSIKTQCLLDGQKTGTVLSASALLL 621
A+ DS G V TF V + + +++CL G K G V L
Sbjct: 261 TDDPT------LAICNDSGGSVFELTFKRV--MGVRTCESRCLFSGSK-GEVCCIEPL-- 320
Query: 622 NESCGSSLPPTLANVAVSTSSIGSMMGGVVGGDSGWKLFNEGSSLVEEGVVIFATHQTAL 681
P L + ++ S+ +M A+ L
Sbjct: 321 ------HSKPELKDHPITQFSLLAM----------------------------ASLTKIL 380
Query: 682 VVRLSPTLEVYAQLSKPDG-VREGSMPYTAWK-CSQSFETSPSEAVERVSLLAIAWDKMV 741
V+ L P+L+V+ ++ P G + S+P AW + + +P A R ++ K
Sbjct: 381 VIGLKPSLKVW--MTFPYGRMDPSSVPLLAWHFVAVNNSVNPMLAFCRGDMVHFLLVKRD 440
Query: 742 QVAKLVKAELKVCGKWSLESAAIGVAWLDDQVLVVLTITGQLFLFEKDGTMIHQTSVFVD 801
+ + + K L I W++ + +V+L +L + ++ Q +
Sbjct: 441 ESGAIHVTKQK---HLHLYYDLINFTWINSRTVVLLDSVEKLHVIDRQ----TQEELETM 500
Query: 802 GFDKEDFIAYHTHFVNVL--GN--------PEKAYHNCVAVRGASIYVLGPMHLIISRLL 861
+ + +HF ++ GN EKA + ++ G I+ LG + + L
Sbjct: 501 EISEVQLVYNSSHFKSLATGGNVSQALALVGEKACYQSISSYGGQIFYLGTKSVYVMMLR 560
Query: 862 PWKERVQVLRKAGDWMGALSMAITIYDGQAHGVIDLPRSLESLQELVVPFLIELLLSYVD 921
W+ER+ L K AL++A + ++G+A V+ L + + +V ++E+L Y D
Sbjct: 561 SWRERMDHLLKQDCLTEALALAWSFHEGKAKAVVGLSGDVSKRKAVVADRMVEILFHYAD 620
Query: 922 EVFSYISVAFCNQIEKNEKSVDVTSGGHSAHSEIKEQYNRVGGVAVEFCVHIRRTDILFD 981
++ + V +++ + V V++C+ ++R D+LF
Sbjct: 621 RALKKC---------PDQGKIQV----------MEQHFQDTVPVIVDYCLLLQRKDLLFG 680
Query: 982 EIFSKFVAVQ-QRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKGWLQRVEQCVLHM 1041
+++ K + FLE LEPYIL D L + P++M+ L+ H+ K L+ VE ++HM
Sbjct: 681 QMYDKLSENSVAKGVFLECLEPYILSDKLVGITPQVMKDLIVHFQDKKLLENVEALIVHM 740
Query: 1042 DISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLQ------TSKSERAS 1101
DI+SLD QVV +C ++ LY A+VY++N+G+++F +P+E+L V+ + ++
Sbjct: 741 DITSLDIQQVVLMCWENRLYDAMVYVYNRGMNEFISPMEKLFKVIAPPLNAGKTLTDEQV 800
Query: 1102 AIGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQPLRDELLQFLLENSDSVDTRSISNKSSE 1161
+G K LVY+ C +G A+P G + V +++++ +FL+ S+ S E
Sbjct: 801 VMGNKLLVYISCCLAGRAYP--LGDIPEDLVPLVKNQVFEFLIR------LHSVEASSEE 860
Query: 1162 IGYLKLYHLLELDTGATLDVLRCAFVEGEIPKTNSSPNGLDDVSMQVQEEKNSTSGRKNV 1221
Y + LL DT L+VL F + + N V Q
Sbjct: 861 EVYPYVRTLLHFDTREFLNVLALTFEDFK--------NDKQAVEYQ-------------- 920
Query: 1222 LIQSIVDALVHVLDQAICQTDRSAGGEDIALVGDWPSKKDLVHLFDFVANYVACGRAT-- 1281
Q IVD L+ V+ + T G LF F+A +A T
Sbjct: 921 --QRIVDILLKVMVENSDFTPSQVG-----------------CLFTFLARQLAKPDNTLF 980
Query: 1282 ASKVVVGQILEHLISNSDIPETENDFLPKVTANSVHSRKREKQVLSLLEVVPETHWNASS 1341
++ + Q+LE L S D +S HS +R++ +L LL+ + S
Sbjct: 981 VNRTLFDQVLEFLCSPDD--------------DSRHS-ERQQVLLELLQAGGIVQFEESR 1040
Query: 1342 VLSMCEEAQFFQVCGLIHSIRHQYASALDSYMKDIDEPIHGFAFINRTLLELNNYERTEF 1401
++ M E+A+F+Q+C ++ HQY +D Y+ D F +I+ +L + + E
Sbjct: 1041 LIRMAEKAEFYQICEFMYEREHQYDKIIDCYLHDPLREEEVFNYIH-NILSIPGHSAEEK 1100
Query: 1402 QAV---VISRIPELFNLNREGTFFLVIDHFNNDASDILSQLRTHPRSLFLYLKTLIEVHL 1461
Q+V ++ + EL +L LV HF+ ++ QL+ + LF +L++L++
Sbjct: 1101 QSVWQKAMNHMEELVSLKPCKAAELVATHFSEQIEVVIGQLQ-NQLLLFKFLRSLLDPRE 1160
Query: 1462 SGSLNFSCLRKDDIFGLNYSTKGLDDYLRKLSDFPKYLSNNPVDVTDDIIELYVELLCQY 1521
+N L+ + I E ++ELLCQ+
Sbjct: 1161 GVHVNQELLQ----------------------------------IPPHITEQFIELLCQF 1220
Query: 1522 ERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDKKFND 1581
+ V++ L+ + YR+E +++ Q+Y++ + A+LLE+ GD A L L L + +
Sbjct: 1221 SPDQVIQTLQVLECYRLEETIQITQKYQLHEVTAYLLEKKGDAHGAFLLLLERLQSRLQE 1280
Query: 1582 LEAAVESIVANGASSGSNDPRHFNSVLKLQEVNDIDVLLHACIGLCQRNTPRLNSEESET 1641
+ E+ + + L+ V D V I LCQRN+ LN ++ E
Sbjct: 1281 MTRQDENTKED---------------ILLKGVEDTMV---ETIALCQRNSQNLNQQQREA 1340
Query: 1642 LWFKLLDSFCEPLIDSYNYRTPAFGENQVQFLNESSGLQKDKEARIVTWRILKSDKAAHI 1701
LWF LL EA + ++ S A H
Sbjct: 1341 LWFPLL------------------------------------EAMMTPQKLSSSAAAPHP 1400
Query: 1702 LKRLFSRFIKEIVEGMIGYVHLPIIMSRLLSD--NGSQEFGDFKLTVLGMLGTFGFERRI 1761
+++ M ++ LP I+ R+L D G + G+ + +LGML TF +E+ +
Sbjct: 1401 HCEALKSLTMQVLNSMAAFIALPSILQRILQDPIYGKGKLGEIQGLILGMLDTFNYEQTL 1427
Query: 1762 LDTAKALIEDDTFYTMSLLKKGASHGYAPRNLVCCIC-NCLLIKSSSSYRVRVFNCGHAT 1821
L+T +L+ D +++ L+ S G P+ C IC + + + VF+CGH
Sbjct: 1461 LETTASLLNQDLHWSLCNLRASVSRGLNPKQDYCSICLQQYKRRQEMADEIIVFSCGHLY 1427
Query: 1822 H---LQCEVLDNEASGGD-FSCPVCIHSNQ--------SQRSRGKALTEYSLVNTFSSGT 1881
H LQ + E G ++C C SN+ S+ +G+ + S V S
Sbjct: 1521 HSFCLQSKECTLEVEGQTRWACHKCSSSNKAGKLSENPSENKKGRITS--SQVKMSPSYH 1427
Query: 1882 QSLSGASVQYPQETDLLELPYTLQQI----------PRFEILTNLQKNQ----------- 1900
QS + +L+ P +Q R +LT L +N+
Sbjct: 1581 QSKGDPPARKANSEPVLD-PQQMQAFDQLCRLYRGSSRLALLTELSQNRGGDSCRPFAGP 1427
BLAST of MS010323 vs. ExPASy Swiss-Prot
Match:
Q9VRX2 (Vacuolar protein sorting-associated protein 8 homolog OS=Drosophila melanogaster OX=7227 GN=Vps8 PE=1 SV=1)
HSP 1 Score: 124.8 bits (312), Expect = 1.1e-26
Identity = 142/621 (22.87%), Postives = 260/621 (41.87%), Query Frame = 0
Query: 820 AYHNCVAVRGASIYVLGPMHLIISRLLPWKERVQVLRKAGDWMGALSMAITIYDGQAHGV 879
A +N V RG +YVLG L I + W ER+ L K W A +A+ Y
Sbjct: 435 ACYNSVVSRGTQLYVLGARSLHIIGVRTWSERISFLVKHHRWQEACQLALDGYIAS---- 494
Query: 880 IDLPRSLESLQELVVPFLIELLLSYVDEVFSYISVAFCNQIEKNEKSVDVTSGGHSAHSE 939
+D PR +E ++ E + A+S
Sbjct: 495 VDRPRKRAQAKERIIMLFKEYI----------------------------------ANSA 554
Query: 940 IKEQYNRVGGVAVEFCVHIRRTDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPP 999
+Y G V + + D+L+ +++ K + + FL+ + +I K+ + S+ P
Sbjct: 555 RAPEY--CLGAIVNCLITVGELDLLWTQLWEK-LHNSSTELFLQHISEHIEKETIHSVNP 614
Query: 1000 EIMQALVEHYSHKGWLQ----RVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKG 1059
I QALV++ WL+ ++EQ +L +D LD NQV++ + H L+ A +YL +
Sbjct: 615 VISQALVDY-----WLEHSPAKLEQLILKLDWMCLDLNQVLKAVKKHRLFRAQIYLNTQA 674
Query: 1060 LDDFRTPLEELLAVLQTSKSERASAIGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQPLRD 1119
L+D+ L ELL ++ +++ +G LVY+ C +G +P G+ + V ++
Sbjct: 675 LNDYTAALTELLPLVTPDETD----LGNCLLVYVSSCLAGREYPSGE--IPVELVHQVKH 734
Query: 1120 ELLQFLLENSDSVDTRSISNKSSEIGYLKLYHLLELDTGATLDVLRCAFVEGEIPKTNSS 1179
++L+ L S N E+ Y L LL+ DT TL+V+ AF E E S+
Sbjct: 735 DVLRCL------TSQHSKENAGDELPYPYLRALLKFDTRETLNVISLAFQEREF----SN 794
Query: 1180 PNGLDDVSMQVQEEKNSTSGRKNVLIQSIVDALVHVLDQAICQTDRSAGGEDIALVGDWP 1239
G+ S RK I++ L+ ++ + +A +I
Sbjct: 795 ELGI--------------SHRKR-----IINLLLEIM------SPENATWAEIGC----- 854
Query: 1240 SKKDLVHLFDFVANYVACGRATASKVVVGQILEHLI-------SNSDIPETENDFLPKVT 1299
L +F+A ++ + ++ ++L HL S+ E EN + ++
Sbjct: 855 -------LLNFIAQQISMQCLPRDRQLLERVLSHLAQEEIANESSRQHSERENAWHELLS 914
Query: 1300 ANSVHSRKREKQVLSLLEVVPETHWNASSVLSMCEEAQFFQVCGLIHSIRHQYASALDSY 1359
+N + +++ L L E+A+ + V + +Y + LDSY
Sbjct: 915 SNCLAEISSDEEQLRL-----------------AEKAKCYCVVEYLLEKLERYDTILDSY 935
Query: 1360 MKDIDEPIHGFAFINRTLLELNNYERTEFQAVVISRIPELFNLNREGTFFLVIDHFNNDA 1419
+++ FA++ R + + +R+ F+ + + EL +N + T L+ H+
Sbjct: 975 IRNEARHETMFAYMER---HVASPKRSIFRQLK-RNLRELLTINAKETTRLLSLHYPEKI 935
Query: 1420 SDILSQLRTHPRSLFLYLKTL 1430
+++L LR L+L+LK L
Sbjct: 1035 NELLDNLRREENLLYLFLKCL 935
BLAST of MS010323 vs. ExPASy Swiss-Prot
Match:
P39702 (Vacuolar protein sorting-associated protein 8 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=VPS8 PE=1 SV=2)
HSP 1 Score: 65.9 bits (159), Expect = 5.9e-09
Identity = 30/157 (19.11%), Postives = 82/157 (52.23%), Query Frame = 0
Query: 971 KFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKGWLQRVEQCVLHMDISSL 1030
+F ++ + E++ + + + S+ P + ++++++Y+ + L+ +E ++ ++ ++L
Sbjct: 529 EFFELKDNAVYFEVVANIVAQGSVTSISPVLFRSIIDYYAKEENLKVIEDLIIMLNPTTL 588
Query: 1031 DFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLQTSKSE----RASAIGYKTL 1090
D + V+LC+ + L+ L+Y++NK DD++TP+ +L+ + + + +T
Sbjct: 589 DVDLAVKLCQKYNLFDLLIYIWNKIFDDYQTPVVDLIYRISNQSEKCVIFNGPQVPPETT 648
Query: 1091 V--YLKYCFSGLAFPPGQGTLAHSRVQPLRDELLQFL 1122
+ Y+ Y +G +P + ++ EL F+
Sbjct: 649 IFDYVTYILTGRQYPQNLSISPSDKCSKIQRELSAFI 685
BLAST of MS010323 vs. ExPASy TrEMBL
Match:
A0A6J1CRK3 (vacuolar protein sorting-associated protein 8 homolog OS=Momordica charantia OX=3673 GN=LOC111013938 PE=3 SV=1)
HSP 1 Score: 3498.8 bits (9071), Expect = 0.0e+00
Identity = 1799/1947 (92.40%), Postives = 1803/1947 (92.60%), Query Frame = 0
Query: 1 MTKELTDTGVLPLMELDLNAFIHAHLSSSDDDDDDDPSFPHRTIDEILNESGSAPSSPPH 60
MTKELTDTGVLPLMELDLNAFIHAHLSS DDDDDDDPSFPHRTIDEIL ESGS PSSPPH
Sbjct: 1 MTKELTDTGVLPLMELDLNAFIHAHLSSGDDDDDDDPSFPHRTIDEILKESGSPPSSPPH 60
Query: 61 SPPYSGDRAVATRDGAVSESPSTPPSKSPFEAPRSNQWNEKSVQLRPAAVSLVKVGELTD 120
SPPY GDRAVATRDG
Sbjct: 61 SPPYGGDRAVATRDG--------------------------------------------- 120
Query: 121 DLFRRGSRPLPSLFGGVRSNAKPGAALAAAAAASRSMPAPHAAAIKSRRAGHGSMVIDDD 180
Sbjct: 121 ------------------------------------------------------------ 180
Query: 181 ELASPSADDSEAVFGGLYSTNTHSEESGENSTVMDDTDADYQVASINVGGGVWARDRIRD 240
NSTVMDDTDADYQVASINVG GVWARDRIRD
Sbjct: 181 -----------------------------NSTVMDDTDADYQVASINVGDGVWARDRIRD 240
Query: 241 DVPDNDEFRITKGLKCVAEQRSVDDVNFDESTTILPPVEANDGSVSDYADKNVCSTELRP 300
DVPDNDEFRITKGLKCVAEQRSVDDVNFDESTTILPPVEANDGSVSDYA+KNVCSTELRP
Sbjct: 241 DVPDNDEFRITKGLKCVAEQRSVDDVNFDESTTILPPVEANDGSVSDYAEKNVCSTELRP 300
Query: 301 SILDVDESNEGAIPSSSEPDNDGSAVDYGSLEWETQDFKKCLQQSKDTEVDLVIKGPSVV 360
SILDVDESNEGAIPSSSEPDNDGSAVDYGSLEWETQDFKKCLQQSKDTEVDLVIK PSVV
Sbjct: 301 SILDVDESNEGAIPSSSEPDNDGSAVDYGSLEWETQDFKKCLQQSKDTEVDLVIKDPSVV 360
Query: 361 NDISELGETTEHMDDSTISEQAEMMLLPSTNPLEAAEEIEKKQAFTALHWEEGVAAQPMK 420
NDISELGETTEHMDDSTISEQAEMMLLPSTNPLEAAEEIEKKQAFTALHWEEGVAAQPMK
Sbjct: 361 NDISELGETTEHMDDSTISEQAEMMLLPSTNPLEAAEEIEKKQAFTALHWEEGVAAQPMK 420
Query: 421 LEGIKGGSTTLGYFDIQADNSISRTISSHSFKREHGFPQVLAVHANYIAVGMSKGNIVVV 480
LEGIKGGSTTLGYFDIQADNSISRTISSHSFKREHGFPQVLAVHANYIAVGMSKGNIVVV
Sbjct: 421 LEGIKGGSTTLGYFDIQADNSISRTISSHSFKREHGFPQVLAVHANYIAVGMSKGNIVVV 480
Query: 481 ASKYSAQNGDNMDAKMLLLGLQGDKSTAPVTSLCFNLQGDLLLAGYNDGHITVWDVLRAS 540
ASKYSAQNGDNMDAKMLLLGLQGDKSTAPVTSLCFNLQGDLLLAGYNDGHITVWDVLRAS
Sbjct: 481 ASKYSAQNGDNMDAKMLLLGLQGDKSTAPVTSLCFNLQGDLLLAGYNDGHITVWDVLRAS 540
Query: 541 AAKIISGEHTSPVVHSLFLGQDAQVTRQFKAVTGDSKGLVLLHTFSVVPLLNRFSIKTQC 600
AAKIISGEHTSPVVHSLFLGQDAQVTRQFKAVTGDSKGLVLLHTFSVVPLLNRFSIKTQC
Sbjct: 541 AAKIISGEHTSPVVHSLFLGQDAQVTRQFKAVTGDSKGLVLLHTFSVVPLLNRFSIKTQC 600
Query: 601 LLDGQKTGTVLSASALLLNESCGSSLPPTLANVAVSTSSIGSMMGGVVGGDSGWKLFNEG 660
LLDGQKTGTVLSASALLLNESCGSSLPPTLANVAVSTSSIGSMMGGVVGGDSGWKLFNEG
Sbjct: 601 LLDGQKTGTVLSASALLLNESCGSSLPPTLANVAVSTSSIGSMMGGVVGGDSGWKLFNEG 660
Query: 661 SSLVEEGVVIFATHQTALVVRLSPTLEVYAQLSKPDGVREGSMPYTAWKCSQSFETSPSE 720
SSLVEEGVVIFATHQTALVVRLSPTLEVYAQLSKPDGVREGSMPYTAWKCSQSFETSPSE
Sbjct: 661 SSLVEEGVVIFATHQTALVVRLSPTLEVYAQLSKPDGVREGSMPYTAWKCSQSFETSPSE 720
Query: 721 AVERVSLLAIAWDKMVQVAKLVKAELKVCGKWSLESAAIGVAWLDDQVLVVLTITGQLFL 780
AVERVSLLAIAWDKMVQVAKLVKAELKVCGKWSLESAAIGVAWLDDQVLVVLTITGQLFL
Sbjct: 721 AVERVSLLAIAWDKMVQVAKLVKAELKVCGKWSLESAAIGVAWLDDQVLVVLTITGQLFL 780
Query: 781 FEKDGTMIHQTSVFVDGFDKEDFIAYHTHFVNVLGNPEKAYHNCVAVRGASIYVLGPMHL 840
FEKDGTMIHQTSVFVDGFDKEDFIAYHTHFVNVLGNPEKAYHNCVAVRGAS+YVLGPMHL
Sbjct: 781 FEKDGTMIHQTSVFVDGFDKEDFIAYHTHFVNVLGNPEKAYHNCVAVRGASVYVLGPMHL 840
Query: 841 IISRLLPWKERVQVLRKAGDWMGALSMAITIYDGQAHGVIDLPRSLESLQELVVPFLIEL 900
IISRLLPWKERVQVLRKAGDWMGALSMAITIYDGQAHGVIDLPRSLESLQELVVPFLIEL
Sbjct: 841 IISRLLPWKERVQVLRKAGDWMGALSMAITIYDGQAHGVIDLPRSLESLQELVVPFLIEL 900
Query: 901 LLSYVDEVFSYISVAFCNQIEKNEKSVDVTSGGHSAHSEIKEQYNRVGGVAVEFCVHIRR 960
LLSYVDEVFSYISVAFCNQIEKNEKSVDVTSGGHSAHSEIKEQYNRVGGVAVEFCVHIRR
Sbjct: 901 LLSYVDEVFSYISVAFCNQIEKNEKSVDVTSGGHSAHSEIKEQYNRVGGVAVEFCVHIRR 960
Query: 961 TDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKGWLQRVEQ 1020
TDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKGWLQRVEQ
Sbjct: 961 TDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKGWLQRVEQ 1020
Query: 1021 CVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLQTSKSERASA 1080
CVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLQTSKSERASA
Sbjct: 1021 CVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLQTSKSERASA 1080
Query: 1081 IGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQPLRDELLQFLLENSDSVDTRSISNKSSEI 1140
IGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQ LRDELLQFLLENSDSVDTRSISNKSSEI
Sbjct: 1081 IGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSDSVDTRSISNKSSEI 1140
Query: 1141 GYLKLYHLLELDTGATLDVLRCAFVEGEIPKTNSSPNGLDDVSMQVQEEKNSTSGRKNVL 1200
GYLKLYHLLELDTGATLDVLRCAFVEGEIPKTNSSPNGLDDVSMQVQEEKNSTSGRKNVL
Sbjct: 1141 GYLKLYHLLELDTGATLDVLRCAFVEGEIPKTNSSPNGLDDVSMQVQEEKNSTSGRKNVL 1200
Query: 1201 IQSIVDALVHVLDQAICQTDRSAGGEDIALVGDWPSKKDLVHLFDFVANYVACGRATASK 1260
IQSIVDALVHVLDQAICQTDRSAGGEDIALVGDWPSKKDLVHLFDFVANYVACG ATASK
Sbjct: 1201 IQSIVDALVHVLDQAICQTDRSAGGEDIALVGDWPSKKDLVHLFDFVANYVACGGATASK 1260
Query: 1261 VVVGQILEHLISNSDIPETENDFLPKVTANSVHSRKREKQVLSLLEVVPETHWNASSVLS 1320
V+VGQILEHLISNSDIPETENDFLPKVTANSVHSRKREKQVLSLLEVVPETHWNASSVLS
Sbjct: 1261 VIVGQILEHLISNSDIPETENDFLPKVTANSVHSRKREKQVLSLLEVVPETHWNASSVLS 1320
Query: 1321 MCEEAQFFQVCGLIHSIRHQYASALDSYMKDIDEPIHGFAFINRTLLELNNYERTEFQAV 1380
MCEEAQFFQVCGLIHSIRHQYASALDSYMKDIDEPIHGFAFINRTLLELNNYERTEFQAV
Sbjct: 1321 MCEEAQFFQVCGLIHSIRHQYASALDSYMKDIDEPIHGFAFINRTLLELNNYERTEFQAV 1380
Query: 1381 VISRIPELFNLNREGTFFLVIDHFNNDASDILSQLRTHPRSLFLYLKTLIEVHLSGSLNF 1440
VISRIPELFNLNREGTFFLVIDHFNNDASDILSQLRTHPRSLFLYLKTLIEVHLSGSLNF
Sbjct: 1381 VISRIPELFNLNREGTFFLVIDHFNNDASDILSQLRTHPRSLFLYLKTLIEVHLSGSLNF 1440
Query: 1441 SCLRKDDIFGLNYSTKGLDDYLRKLSDFPKYLSNNPVDVTDDIIELYVELLCQYERESVL 1500
SCLRKDDIFGLNYSTKGLDDYLRKLSDFPKYLSNNPVDVTDDIIELYVELLCQYERESVL
Sbjct: 1441 SCLRKDDIFGLNYSTKGLDDYLRKLSDFPKYLSNNPVDVTDDIIELYVELLCQYERESVL 1500
Query: 1501 KFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDKKFNDLEAAVE 1560
KFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDKKFNDLEAAVE
Sbjct: 1501 KFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDKKFNDLEAAVE 1560
Query: 1561 SIVANGASSGSNDPRHFNSVLKLQEVNDIDVLLHACIGLCQRNTPRLNSEESETLWFKLL 1620
SIVANGASSGSNDPRHFNSVLKLQEVNDIDVLLHACIGLCQRNTPRLNSEESETLWFKLL
Sbjct: 1561 SIVANGASSGSNDPRHFNSVLKLQEVNDIDVLLHACIGLCQRNTPRLNSEESETLWFKLL 1620
Query: 1621 DSFCEPLIDSYNYRTPAFGENQVQFLNESSGLQKDKEARIVTWRILKSDKAAHILKRLFS 1680
DSFCEPLIDSYNYRTPAFGENQVQFLNESSGLQKDKEARIVTWRILKSDKAAHILKRLFS
Sbjct: 1621 DSFCEPLIDSYNYRTPAFGENQVQFLNESSGLQKDKEARIVTWRILKSDKAAHILKRLFS 1680
Query: 1681 RFIKEIVEGMIGYVHLPIIMSRLLSDNGSQEFGDFKLTVLGMLGTFGFERRILDTAKALI 1740
RFIKEIVEGMIGYVHLPIIMSRLLSDNGSQEFGDFKLTVLGMLGTFGFERRILDTAKALI
Sbjct: 1681 RFIKEIVEGMIGYVHLPIIMSRLLSDNGSQEFGDFKLTVLGMLGTFGFERRILDTAKALI 1740
Query: 1741 EDDTFYTMSLLKKGASHGYAPRNLVCCICNCLLIKSSSSYRVRVFNCGHATHLQCEVLDN 1800
EDDTFYTMSLLKKGASHGYAPRNLVCCICNCLLIKSSSSYRVRVFNCGHATHLQCEVLDN
Sbjct: 1741 EDDTFYTMSLLKKGASHGYAPRNLVCCICNCLLIKSSSSYRVRVFNCGHATHLQCEVLDN 1800
Query: 1801 EASGGDFSCPVCIHSNQSQRSRGKALTEYSLVNTFSSGTQSLSGASVQYPQETDLLELPY 1860
EASGGDFSCPVCIHSNQSQR RGKALTEYSLVNTFSSGTQSLSGASVQYPQETDLLELPY
Sbjct: 1801 EASGGDFSCPVCIHSNQSQRPRGKALTEYSLVNTFSSGTQSLSGASVQYPQETDLLELPY 1813
Query: 1861 TLQQIPRFEILTNLQKNQRVIEIENVPQLRLAPPAVYHDKVTKGYHLLVGESSSGIEKQN 1920
TLQQIPRFEILTNLQKNQRVIEIENVPQLRLAPPAVYHDKVTKGYHLLVGESSSGIEKQN
Sbjct: 1861 TLQQIPRFEILTNLQKNQRVIEIENVPQLRLAPPAVYHDKVTKGYHLLVGESSSGIEKQN 1813
Query: 1921 KSKQLREVKVKRRSSLRFPLKASLFGE 1948
KSKQLREVKVKR SSLRFPLKASLFG+
Sbjct: 1921 KSKQLREVKVKRPSSLRFPLKASLFGK 1813
BLAST of MS010323 vs. ExPASy TrEMBL
Match:
A0A6J1EC21 (vacuolar protein sorting-associated protein 8 homolog isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431840 PE=3 SV=1)
HSP 1 Score: 3247.2 bits (8418), Expect = 0.0e+00
Identity = 1676/1964 (85.34%), Postives = 1776/1964 (90.43%), Query Frame = 0
Query: 1 MTKELTDTGVLPLMELDLNAFIHAHLSSSDDDDDDDP---SFPHRTIDEILNES----GS 60
MTKELTDT LP MELDLNAFIHAHLSS DDDDDDD SFPHR+IDEILNES S
Sbjct: 1 MTKELTDTETLPPMELDLNAFIHAHLSSGDDDDDDDEDDLSFPHRSIDEILNESSSSTSS 60
Query: 61 APSSPPHSPPYSGDRAVATRDGAVSESPSTPPSKSPFE-------APRSNQWNEKSVQLR 120
+PSSPP+SPP R++A RDG S S S PP KSPFE PRSNQ NEKSVQL+
Sbjct: 61 SPSSPPNSPPPRDHRSIAARDGRASASRSIPPFKSPFEEIIKASKVPRSNQRNEKSVQLK 120
Query: 121 PAAVSLVKVGELTDDLFRRGSRPLPSLFGGVRSNAKPGAALAAAAAASRSMPAPHAAAIK 180
P +VS KVGELTDD FRRGSR LPSLFGGVRSNAKPGAALAAAAAASRSMPAPHAAAIK
Sbjct: 121 PGSVSHTKVGELTDDPFRRGSRALPSLFGGVRSNAKPGAALAAAAAASRSMPAPHAAAIK 180
Query: 181 SRRAGHGSMVIDDDELASPSADDSEAVFGGLYSTNTHSEESGENSTVMDDTDADYQVASI 240
SRR+GHGS+V+DDDELAS SA DSE VF LY T HS+ES E S + + +ADYQ AS+
Sbjct: 181 SRRSGHGSVVLDDDELASSSAVDSEFVFDDLYPTIDHSKESREKSISLVERNADYQGASV 240
Query: 241 NVGGGVWARDRIRDDVPDNDEFRITKGLKCVAEQRSVDDVNFDESTTILPPVEANDGSVS 300
NVGG WARD IRD V NDEFRITK +C AEQ VDDVNFDES+T LPPVEAN S+S
Sbjct: 241 NVGGEFWARDNIRDCVLYNDEFRITKDTECEAEQSFVDDVNFDESSTTLPPVEANGRSLS 300
Query: 301 DYADKNVCSTELRPSILDVDESNEGAIPSSSEPDNDGSAVDYGSLEWETQDFKKCLQQSK 360
D AD NVCS + P++LDVDESNEGA PSS +PD D SAV YG LE ETQDF+K Q SK
Sbjct: 301 DSADNNVCSMDAEPTVLDVDESNEGAFPSSPKPDYDRSAVGYGRLELETQDFEKRFQPSK 360
Query: 361 DTEVDLVIKGPSVVNDISELGETTEHMDDSTISEQAEMMLLPSTNPLEAAEEIEKKQAFT 420
D+EV L I+ SVVNDISE ET E +D+ E+AE M L S+NPLE AEEIEKKQAFT
Sbjct: 361 DSEV-LAIEDLSVVNDISESRETAEQLDNFHTGERAETMSLSSSNPLELAEEIEKKQAFT 420
Query: 421 ALHWEEGVAAQPMKLEGIKGGSTTLGYFDIQADNSISRTISSHSFKREHGFPQVLAVHAN 480
ALHWEEGVAAQPM+LEGIKGG+T LGYFDIQADNSISRTISSHSFKREHGFPQ LAVHAN
Sbjct: 421 ALHWEEGVAAQPMRLEGIKGGTTALGYFDIQADNSISRTISSHSFKREHGFPQALAVHAN 480
Query: 481 YIAVGMSKGNIVVVASKYSAQNGDNMDAKMLLLGLQGDKSTAPVTSLCFNLQGDLLLAGY 540
YIAVGMSKGNIVVVASKYSAQNGDNMDAKMLLLG QGDKSTAPVTSLCFN QGDLLLAGY
Sbjct: 481 YIAVGMSKGNIVVVASKYSAQNGDNMDAKMLLLGSQGDKSTAPVTSLCFNQQGDLLLAGY 540
Query: 541 NDGHITVWDVLRASAAKIISGEHTSPVVHSLFLGQDAQVTRQFKAVTGDSKGLVLLHTFS 600
+DG +TVWDVLRA+AAK+ISGEHTSPVVHSLFLGQ+AQVTRQFKAVTGDSKGLVLLHTFS
Sbjct: 541 SDGQVTVWDVLRATAAKVISGEHTSPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFS 600
Query: 601 VVPLLNRFSIKTQCLLDGQKTGTVLSASALLLNESCGSSLPPTLANVAVSTSSIGSMMGG 660
VVPLLNRFSIKTQCLLDGQKTGTVLSASALLLNE CGSSLPP+L+NVAVSTSSIGSMMGG
Sbjct: 601 VVPLLNRFSIKTQCLLDGQKTGTVLSASALLLNEFCGSSLPPSLSNVAVSTSSIGSMMGG 660
Query: 661 VVGGDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSPTLEVYAQLSKPDGVREGSMPYT 720
VVGGDSGWKLFNEGSSLV EGVVIFATHQTALVVRLSPT+EVYA+LSKPDG++EGSMPYT
Sbjct: 661 VVGGDSGWKLFNEGSSLV-EGVVIFATHQTALVVRLSPTVEVYARLSKPDGIQEGSMPYT 720
Query: 721 AWKCSQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKAELKVCGKWSLESAAIGVAWLDD 780
AWKCSQS ETSPSEAVER+SLLAIAWDKMVQVAKLVK ELKVCGKWSLESAAIGVAWLDD
Sbjct: 721 AWKCSQSIETSPSEAVERISLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVAWLDD 780
Query: 781 QVLVVLTITGQLFLFEKDGTMIHQTSVFVDGFDKEDFIAYHTHFVNVLGNPEKAYHNCVA 840
QVLV+LT+TGQLFLFEKDGTMIHQTSVFVDGFDKEDFIA+HTHFVNVLGNPEKAYHNCVA
Sbjct: 781 QVLVILTVTGQLFLFEKDGTMIHQTSVFVDGFDKEDFIAHHTHFVNVLGNPEKAYHNCVA 840
Query: 841 VRGASIYVLGPMHLIISRLLPWKERVQVLRKAGDWMGALSMAITIYDGQAHGVIDLPRSL 900
VRGAS+YVLGP HL+ISRLLPWKERVQVLRKAGDWM ALSMAITIYDG AHGVIDLPRSL
Sbjct: 841 VRGASVYVLGPKHLVISRLLPWKERVQVLRKAGDWMTALSMAITIYDGHAHGVIDLPRSL 900
Query: 901 ESLQELVVPFLIELLLSYVDEVFSYISVAFCNQIEKNEKSVDVTSGGHSAHSEIKEQYNR 960
ESLQELV+PFLIELLLSYVDEVFSYIS+AFCNQIEKNEK DVTSG HSAHSEIKEQYNR
Sbjct: 901 ESLQELVMPFLIELLLSYVDEVFSYISLAFCNQIEKNEKLDDVTSGSHSAHSEIKEQYNR 960
Query: 961 VGGVAVEFCVHIRRTDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQALV 1020
VGGVAVEFCVHI RTDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQALV
Sbjct: 961 VGGVAVEFCVHITRTDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQALV 1020
Query: 1021 EHYSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL 1080
EHYS KGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL
Sbjct: 1021 EHYSQKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL 1080
Query: 1081 LAVLQTSKSERASAIGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQPLRDELLQFLLENSD 1140
LAVLQTSKS+RASAIGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQ LRDELLQFLLENSD
Sbjct: 1081 LAVLQTSKSKRASAIGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSD 1140
Query: 1141 SVDTRSISNKSSEIGYLKLYHLLELDTGATLDVLRCAFVEGEIPKTNSSPNGLDDVSMQV 1200
+VDTRSISNKSSE+GYL LYHLLELDTGATLDVLRCAFVEGEI K +SS +G D SMQV
Sbjct: 1141 TVDTRSISNKSSEVGYLNLYHLLELDTGATLDVLRCAFVEGEIVKADSSLDGSVDASMQV 1200
Query: 1201 QEEKNSTSGRKNVLIQSIVDALVHVLDQAICQTDRSAGGEDIALVGDWPSKKDLVHLFDF 1260
Q+E KN L+Q++VDAL+H+LD+AI QT S GG++I LV DWPSKKDL HLFDF
Sbjct: 1201 QKE-------KNFLVQNVVDALIHILDKAISQTYGSPGGDNITLVEDWPSKKDLFHLFDF 1260
Query: 1261 VANYVACGRATASKVVVGQILEHLISNSDIPETENDFLPKVTANSVHSRKREKQVLSLLE 1320
VANYVACG+AT SK VVGQILEHLISNSDIPE E DF+ VTANSVHSRKREKQVLSLLE
Sbjct: 1261 VANYVACGKATVSKDVVGQILEHLISNSDIPEMEIDFVHSVTANSVHSRKREKQVLSLLE 1320
Query: 1321 VVPETHWNASSVLSMCEEAQFFQVCGLIHSIRHQYASALDSYMKDIDEPIHGFAFINRTL 1380
V+PETHWN SSVL MCE+AQFFQVCGLIHSI HQY+SALD YMKD++EPIH FAFINRTL
Sbjct: 1321 VIPETHWNPSSVLRMCEKAQFFQVCGLIHSISHQYSSALDGYMKDVEEPIHAFAFINRTL 1380
Query: 1381 LELNNYERTEFQAVVISRIPELFNLNREGTFFLVIDHFNNDASDILSQLRTHPRSLFLYL 1440
+EL+ E+TEF+ VVISRIPEL NLNREGTFFLVIDHF ND SDILSQL HPRSLFLYL
Sbjct: 1381 MELSYSEQTEFRGVVISRIPELLNLNREGTFFLVIDHFGNDVSDILSQLHNHPRSLFLYL 1440
Query: 1441 KTLIEVHLSGSLNFSCLRKDDIFGLNYSTKGLDDYLRKLSDFPKYLSNNPVDVTDDIIEL 1500
KTLIEVH SG+LNFSCL+KDD FG+NYSTKGLDDYL+KLSDFPK+LSNNPVDVTDDIIEL
Sbjct: 1441 KTLIEVHQSGNLNFSCLKKDDNFGVNYSTKGLDDYLQKLSDFPKFLSNNPVDVTDDIIEL 1500
Query: 1501 YVELLCQYERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS 1560
YVELLCQYERESVLKFLETFDSY VEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS
Sbjct: 1501 YVELLCQYERESVLKFLETFDSYHVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS 1560
Query: 1561 SLDKKFNDLEAAVESIVANGASSGSNDPRHFNSVLKLQEVNDIDVLLHACIGLCQRNTPR 1620
SLDKKF+DLEAAV IV NGASSGSND + F+SVLKLQEVNDI VLLHACIGLCQRNTPR
Sbjct: 1561 SLDKKFHDLEAAVGGIVTNGASSGSNDSQLFSSVLKLQEVNDIYVLLHACIGLCQRNTPR 1620
Query: 1621 LNSEESETLWFKLLDSFCEPLIDSYNYRTPAFGENQVQFLNESSGLQKDKEARIVTWRIL 1680
LNSEESETLWFKLLDSFCEPLI+S+NYRT +FGENQVQFLNESSG QKDKEA IVTWRIL
Sbjct: 1621 LNSEESETLWFKLLDSFCEPLIESFNYRTASFGENQVQFLNESSGSQKDKEAHIVTWRIL 1680
Query: 1681 KSDKAAHILKRLFSRFIKEIVEGMIGYVHLPIIMSRLLSDNGSQEFGDFKLTVLGMLGTF 1740
KS++ AHIL+ LFSRFI+EIVEGMIGYVHLP IMSRLLSDNGSQEFGDFKLT+LGMLGTF
Sbjct: 1681 KSNQTAHILRSLFSRFIREIVEGMIGYVHLPTIMSRLLSDNGSQEFGDFKLTILGMLGTF 1740
Query: 1741 GFERRILDTAKALIEDDTFYTMSLLKKGASHGYAPRNLVCCICNCLLIKSSSSYRVRVFN 1800
GFERRILDTAKALIEDDTFYTMSLLKKGASHGYAPR VCCICN LL+KSSSSYRVRVFN
Sbjct: 1741 GFERRILDTAKALIEDDTFYTMSLLKKGASHGYAPRGAVCCICNRLLVKSSSSYRVRVFN 1800
Query: 1801 CGHATHLQCEVLDNEASGGDFSCPVCIHSNQSQRSRGKALTEYSLVNTFSSGTQSLSGAS 1860
CGHATHLQCEVLDNEASGGDFSCPVC+HSN SQRSRGKALTEYSLVN FSS TQS SGA
Sbjct: 1801 CGHATHLQCEVLDNEASGGDFSCPVCVHSNHSQRSRGKALTEYSLVNKFSSRTQSSSGAF 1860
Query: 1861 VQYPQETDLLELPYTLQQIPRFEILTNLQKNQRVIEIENVPQLRLAPPAVYHDKVTKGYH 1920
V YPQETDLLELPYTLQQIPRFEIL NLQKNQRVI+IEN+PQLRLAPPAVYHDKVTKGYH
Sbjct: 1861 VSYPQETDLLELPYTLQQIPRFEILANLQKNQRVIDIENMPQLRLAPPAVYHDKVTKGYH 1920
Query: 1921 LLVGESSSGI---EKQNKSKQLREVKVKRRSSLRFPLKASLFGE 1948
LLVG+SS GI EK NKS+QL+EVKVKR SSLRFPLKA+LFG+
Sbjct: 1921 LLVGDSSGGIEKVEKLNKSRQLKEVKVKRPSSLRFPLKANLFGK 1955
BLAST of MS010323 vs. ExPASy TrEMBL
Match:
A0A6J1IVQ0 (vacuolar protein sorting-associated protein 8 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479042 PE=3 SV=1)
HSP 1 Score: 3240.7 bits (8401), Expect = 0.0e+00
Identity = 1668/1961 (85.06%), Postives = 1775/1961 (90.52%), Query Frame = 0
Query: 1 MTKELTDTGVLPLMELDLNAFIHAHLSSSDDDDDDDPSFPHRTIDEILNES----GSAPS 60
MTK+LTDT LP MELDLNAFIHAHLSS DDDD+DD SFPHR+IDEILNES S+PS
Sbjct: 1 MTKQLTDTETLPPMELDLNAFIHAHLSSGDDDDEDDLSFPHRSIDEILNESSSSTSSSPS 60
Query: 61 SPPHSPPYSGDRAVATRDGAVSESPSTPPSKSPFE-------APRSNQWNEKSVQLRPAA 120
SPP+ PP R++A RDG S S S PP KSPFE PRSNQ NEKSVQL+P +
Sbjct: 61 SPPNLPPPRARRSIAARDGRASASHSIPPFKSPFEEIIKASKVPRSNQRNEKSVQLKPGS 120
Query: 121 VSLVKVGELTDDLFRRGSRPLPSLFGGVRSNAKPGAALAAAAAASRSMPAPHAAAIKSRR 180
VS KVGELTDD FRRGSR LPSLFGGVRSNAKPGAALAAAAAASRSMPAPHAAAIKSRR
Sbjct: 121 VSHTKVGELTDDPFRRGSRALPSLFGGVRSNAKPGAALAAAAAASRSMPAPHAAAIKSRR 180
Query: 181 AGHGSMVIDDDELASPSADDSEAVFGGLYSTNTHSEESGENSTVMDDTDADYQVASINVG 240
+GHGS+V+DDDELAS SA DSE VF LY T HS+ES E S + + +ADYQ AS G
Sbjct: 181 SGHGSVVLDDDELASSSAVDSEFVFDDLYPTIDHSKESREKSISLVERNADYQGAS---G 240
Query: 241 GGVWARDRIRDDVPDNDEFRITKGLKCVAEQRSVDDVNFDESTTILPPVEANDGSVSDYA 300
G WARD IRD V NDEFRITK +C AEQ VDDVNF+ES+T LPPVEAN S+SD A
Sbjct: 241 GEFWARDNIRDCVLYNDEFRITKDTECEAEQSFVDDVNFNESSTTLPPVEANGRSLSDSA 300
Query: 301 DKNVCSTELRPSILDVDESNEGAIPSSSEPDNDGSAVDYGSLEWETQDFKKCLQQSKDTE 360
D NVCS + P++LDVDESNEGA S +PD D SAV YG LE ETQDF+K Q SKD+E
Sbjct: 301 DNNVCSMDAEPTVLDVDESNEGAFSCSPKPDYDKSAVGYGRLELETQDFEKRFQPSKDSE 360
Query: 361 VDLVIKGPSVVNDISELGETTEHMDDSTISEQAEMMLLPSTNPLEAAEEIEKKQAFTALH 420
V L I+ SVVNDISE ET E +D+ E+AE M L STNPLE AEEIEKKQAFTALH
Sbjct: 361 V-LAIEDLSVVNDISESRETAEQLDNFDTGERAETMSLSSTNPLELAEEIEKKQAFTALH 420
Query: 421 WEEGVAAQPMKLEGIKGGSTTLGYFDIQADNSISRTISSHSFKREHGFPQVLAVHANYIA 480
WEEGVAAQPM+LEGIKGG+T LGYFDIQADN ISRTISSHSFKREHGFPQVLAVH NYIA
Sbjct: 421 WEEGVAAQPMRLEGIKGGTTALGYFDIQADNCISRTISSHSFKREHGFPQVLAVHTNYIA 480
Query: 481 VGMSKGNIVVVASKYSAQNGDNMDAKMLLLGLQGDKSTAPVTSLCFNLQGDLLLAGYNDG 540
VGMSKGNIVVVASKYSAQNGDNMD KMLLLG QGDKSTAPVTSLCFN QGDLLLAGY+DG
Sbjct: 481 VGMSKGNIVVVASKYSAQNGDNMDGKMLLLGSQGDKSTAPVTSLCFNQQGDLLLAGYSDG 540
Query: 541 HITVWDVLRASAAKIISGEHTSPVVHSLFLGQDAQVTRQFKAVTGDSKGLVLLHTFSVVP 600
+TVWDVLRA+AAK+ISGEHTSPVVHSLFLGQ+AQVTRQFKAVTGDSKGLVLLHTFSVVP
Sbjct: 541 QVTVWDVLRATAAKVISGEHTSPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFSVVP 600
Query: 601 LLNRFSIKTQCLLDGQKTGTVLSASALLLNESCGSSLPPTLANVAVSTSSIGSMMGGVVG 660
LLNRFSIKTQCLLDGQKTGTVLSASALLLNE CGSSLPP+L+NVAVSTSSIGSMMGGVVG
Sbjct: 601 LLNRFSIKTQCLLDGQKTGTVLSASALLLNEFCGSSLPPSLSNVAVSTSSIGSMMGGVVG 660
Query: 661 GDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSPTLEVYAQLSKPDGVREGSMPYTAWK 720
GDSGWKLFNEGSSLV EGVVIFATHQTALVVRLSPT+EVYA+LSKPDG++EGSMPYTAWK
Sbjct: 661 GDSGWKLFNEGSSLV-EGVVIFATHQTALVVRLSPTVEVYARLSKPDGIQEGSMPYTAWK 720
Query: 721 CSQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKAELKVCGKWSLESAAIGVAWLDDQVL 780
CSQS ETSPSEAVER+SLLAIAWDKMVQVAKLVK ELKVCGKWSLESAAIGVAWLDDQVL
Sbjct: 721 CSQSIETSPSEAVERISLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVAWLDDQVL 780
Query: 781 VVLTITGQLFLFEKDGTMIHQTSVFVDGFDKEDFIAYHTHFVNVLGNPEKAYHNCVAVRG 840
V+LT+TGQLFLFEKDGTMIHQTSVFVDGFDKEDFIA+HTHFVNVLGNPEKAYHNCVAVRG
Sbjct: 781 VILTVTGQLFLFEKDGTMIHQTSVFVDGFDKEDFIAHHTHFVNVLGNPEKAYHNCVAVRG 840
Query: 841 ASIYVLGPMHLIISRLLPWKERVQVLRKAGDWMGALSMAITIYDGQAHGVIDLPRSLESL 900
AS+YVLGP HL+ISRLLPWKERVQVLRKAGDWM ALSMAITIYDG AHGVIDLPRSLESL
Sbjct: 841 ASVYVLGPKHLVISRLLPWKERVQVLRKAGDWMTALSMAITIYDGHAHGVIDLPRSLESL 900
Query: 901 QELVVPFLIELLLSYVDEVFSYISVAFCNQIEKNEKSVDVTSGGHSAHSEIKEQYNRVGG 960
QELV+PFLIELLLSYVDEVFSYISVAFCNQIEKNEK DVTSG HS HSEIKEQYNRVGG
Sbjct: 901 QELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDVTSGSHSEHSEIKEQYNRVGG 960
Query: 961 VAVEFCVHIRRTDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHY 1020
VAVEFCVHI RTDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHY
Sbjct: 961 VAVEFCVHITRTDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHY 1020
Query: 1021 SHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAV 1080
S +GWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAV
Sbjct: 1021 SQRGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAV 1080
Query: 1081 LQTSKSERASAIGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQPLRDELLQFLLENSDSVD 1140
LQTSKS+RASAIGYK LVYLKYCFSGLAFPPGQGTL+HSRVQ LRDELLQFLLENSD+VD
Sbjct: 1081 LQTSKSKRASAIGYKILVYLKYCFSGLAFPPGQGTLSHSRVQSLRDELLQFLLENSDTVD 1140
Query: 1141 TRSISNKSSEIGYLKLYHLLELDTGATLDVLRCAFVEGEIPKTNSSPNGLDDVSMQVQEE 1200
TRSISNKSSE+GYL LYHLL+LDTGATLDVLRCAFVEGEI K +SS +G D S+Q Q+E
Sbjct: 1141 TRSISNKSSEVGYLNLYHLLKLDTGATLDVLRCAFVEGEIVKADSSLDGSVDASIQAQKE 1200
Query: 1201 KNSTSGRKNVLIQSIVDALVHVLDQAICQTDRSAGGEDIALVGDWPSKKDLVHLFDFVAN 1260
KNSTSGRKN L+Q++VDALVH+LD+AI QT RS GG++I LV DWPSKK+L HLFDFVAN
Sbjct: 1201 KNSTSGRKNFLVQNVVDALVHILDKAISQTYRSPGGDNITLVEDWPSKKNLFHLFDFVAN 1260
Query: 1261 YVACGRATASKVVVGQILEHLISNSDIPETENDFLPKVTANSVHSRKREKQVLSLLEVVP 1320
YV CG+ATASK VVGQILEHLISNSDIPE E DF+ VTANSVHSRKRE+QVLSLLEV+P
Sbjct: 1261 YVVCGKATASKDVVGQILEHLISNSDIPEMEIDFVHSVTANSVHSRKRERQVLSLLEVIP 1320
Query: 1321 ETHWNASSVLSMCEEAQFFQVCGLIHSIRHQYASALDSYMKDIDEPIHGFAFINRTLLEL 1380
ETHWN SSVL MCE+AQFFQVCGLIHSI HQY+SALD YMKD+DEPIH FAFINRTL+EL
Sbjct: 1321 ETHWNPSSVLRMCEKAQFFQVCGLIHSISHQYSSALDGYMKDVDEPIHAFAFINRTLMEL 1380
Query: 1381 NNYERTEFQAVVISRIPELFNLNREGTFFLVIDHFNNDASDILSQLRTHPRSLFLYLKTL 1440
NN E+TEF+ VVISRIPEL NLNREGTFFLVIDHF+ND SDILSQL HPRSLFLYLKTL
Sbjct: 1381 NNSEQTEFRGVVISRIPELLNLNREGTFFLVIDHFSNDVSDILSQLHNHPRSLFLYLKTL 1440
Query: 1441 IEVHLSGSLNFSCLRKDDIFGLNYSTKGLDDYLRKLSDFPKYLSNNPVDVTDDIIELYVE 1500
IEVH SG+LNFSCL+KDD FG+NYSTKGLDDYL+KLSDFPK+LSNNPVDVTDDIIELYVE
Sbjct: 1441 IEVHQSGNLNFSCLKKDDNFGVNYSTKGLDDYLQKLSDFPKFLSNNPVDVTDDIIELYVE 1500
Query: 1501 LLCQYERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLD 1560
LLCQYERESVLKFLETFDSY VEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLD
Sbjct: 1501 LLCQYERESVLKFLETFDSYHVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLD 1560
Query: 1561 KKFNDLEAAVESIVANGASSGSNDPRHFNSVLKLQEVNDIDVLLHACIGLCQRNTPRLNS 1620
KKF+DLEAAV IV NGASSGSND + F+SVLKLQEVNDI VLLHACIGLCQRNTPRLNS
Sbjct: 1561 KKFHDLEAAVGGIVTNGASSGSNDSQLFSSVLKLQEVNDIYVLLHACIGLCQRNTPRLNS 1620
Query: 1621 EESETLWFKLLDSFCEPLIDSYNYRTPAFGENQVQFLNESSGLQKDKEARIVTWRILKSD 1680
EESETLWFKLLDSFCEPLI+S+NYRT +FGENQVQFLNESSG QKDKEA IVTWRILKS+
Sbjct: 1621 EESETLWFKLLDSFCEPLIESFNYRTASFGENQVQFLNESSGSQKDKEAHIVTWRILKSN 1680
Query: 1681 KAAHILKRLFSRFIKEIVEGMIGYVHLPIIMSRLLSDNGSQEFGDFKLTVLGMLGTFGFE 1740
+ AHIL+ LFSRFI+EIVEGMIGYVHLP IMSRLLSDNGSQEFGDFKLT+LGMLGTFGFE
Sbjct: 1681 QTAHILRSLFSRFIREIVEGMIGYVHLPTIMSRLLSDNGSQEFGDFKLTILGMLGTFGFE 1740
Query: 1741 RRILDTAKALIEDDTFYTMSLLKKGASHGYAPRNLVCCICNCLLIKSSSSYRVRVFNCGH 1800
RRILDTAKALIEDDTFYTMSLLKKGASHGYAPR VCCICN LL+KSSSSYRVRVFNCGH
Sbjct: 1741 RRILDTAKALIEDDTFYTMSLLKKGASHGYAPRGAVCCICNRLLVKSSSSYRVRVFNCGH 1800
Query: 1801 ATHLQCEVLDNEASGGDFSCPVCIHSNQSQRSRGKALTEYSLVNTFSSGTQSLSGASVQY 1860
ATHLQCEVLDNEASGGDFSC VC+HSN SQRSRGKALTEYSLVN FSS TQS SGASV Y
Sbjct: 1801 ATHLQCEVLDNEASGGDFSCQVCVHSNHSQRSRGKALTEYSLVNKFSSRTQSSSGASVSY 1860
Query: 1861 PQETDLLELPYTLQQIPRFEILTNLQKNQRVIEIENVPQLRLAPPAVYHDKVTKGYHLLV 1920
PQETDLLELPYTLQQIPRFEIL NLQKNQRVI+IEN+PQLRLAPPAVYHDKVTKG+HLLV
Sbjct: 1861 PQETDLLELPYTLQQIPRFEILANLQKNQRVIDIENMPQLRLAPPAVYHDKVTKGFHLLV 1920
Query: 1921 GESSSG---IEKQNKSKQLREVKVKRRSSLRFPLKASLFGE 1948
G+SS G +EK NKS+QL+EVKVK+ SSLRFPLKA+LFG+
Sbjct: 1921 GDSSGGVEKVEKLNKSRQLKEVKVKKPSSLRFPLKANLFGK 1956
BLAST of MS010323 vs. ExPASy TrEMBL
Match:
A0A0A0L2X7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G116870 PE=3 SV=1)
HSP 1 Score: 3206.0 bits (8311), Expect = 0.0e+00
Identity = 1642/1955 (83.99%), Postives = 1765/1955 (90.28%), Query Frame = 0
Query: 1 MTKELTDTGVLPLMELDLNAFIHAHLSS-SDDDDDDDPSFPHRTIDEILNESGS----AP 60
MT+ELTDT LP MELDLNAFIHAHLSS DDDDDDD SFPHR+IDEILN+S S +P
Sbjct: 1 MTEELTDTETLPPMELDLNAFIHAHLSSGGDDDDDDDLSFPHRSIDEILNDSSSSTSPSP 60
Query: 61 SSPPHSPPYSGDRAVATRDGAVSESPSTPPSKSPFEAPRSNQWNEKSVQLRPAAVSLVKV 120
SS PH PP G R + D VS SPST P K EA R+N WNEKS QL+P S KV
Sbjct: 61 SSSPHFPPPRGRRNIVAGDDGVSASPSTSPYKDS-EAARNNPWNEKSAQLKPGTASHSKV 120
Query: 121 GELTDDLFRRGSRPLPSLFGGVRSNAKPGAALAAAAAASRSMPAPHAAAIKSRRAGHGSM 180
GELTDD FRRGSRPLPSLFG VRSNAKPGAALAAAAAASRS PAPHAAAIKSRRAG+G+M
Sbjct: 121 GELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIKSRRAGYGNM 180
Query: 181 VIDDDELASPSADDSEAVFGGLYSTNTHSEESGENSTVMDDTDADYQVASINVGGGVWAR 240
V+DDDELAS SA DSE LY N HS+ESGENS + D DYQ+AS+NV G +WA
Sbjct: 181 VLDDDELASSSAVDSEFFSDSLYHANIHSKESGENSISVVDRITDYQIASMNVSGELWAT 240
Query: 241 DRIRDDVPDNDEFRITKGLKCVAEQRSVDDVNFDESTTILPPVEANDGSVSDYADKNVCS 300
+ IRD VP NDEFR+T+ ++ AE SVDDVNF ES + +PPVE ND S+ A+KNVCS
Sbjct: 241 NNIRDGVPHNDEFRMTEDMEFEAETSSVDDVNFKESLSTVPPVETNDRSLLGPAEKNVCS 300
Query: 301 TELRPSILDVDESNEGAIPSSSEPDNDGSAVDYGSLEWETQDFKKCLQQSKDTEVDLVIK 360
T+ P+ LDVDESNEGAIP +EPD++ SAV YGSLE ETQDF+K Q SKDTEVDL I+
Sbjct: 301 TDAHPTELDVDESNEGAIPRPTEPDDEESAVGYGSLELETQDFEKYHQPSKDTEVDLAIE 360
Query: 361 GPSVVNDISELGETTEHMDDSTISEQAEMMLLPSTNPLEAAEEIEKKQAFTALHWEEGVA 420
PS+VNDI E GETTE D+ I ++ EM+ + STNPL+ AEEIEKKQAFTALHWEEGVA
Sbjct: 361 DPSIVNDIIESGETTEQPDNLQIGKRPEMISVSSTNPLDLAEEIEKKQAFTALHWEEGVA 420
Query: 421 AQPMKLEGIKGGSTTLGYFDIQADNSISRTISSHSFKREHGFPQVLAVHANYIAVGMSKG 480
AQPM+LEGIKG +TTLGYFDIQADNSISRTISSHSF+REHGFPQVLAVHANYIAVGMSKG
Sbjct: 421 AQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHANYIAVGMSKG 480
Query: 481 NIVVVASKYSAQNGDNMDAKMLLLGLQGDKSTAPVTSLCFNLQGDLLLAGYNDGHITVWD 540
NIVVVASKYSAQNGDNMDAKM+LLG QGDKSTAP TSLCF+ QGDLLLAGY+DGHITVWD
Sbjct: 481 NIVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPATSLCFSQQGDLLLAGYSDGHITVWD 540
Query: 541 VLRASAAKIISGEHTSPVVHSLFLGQDAQVTRQFKAVTGDSKGLVLLHTFSVVPLLNRFS 600
VLRASAAK+ISGEH SPVVHSLFLGQ+AQVTRQFKAVTGDSKGLVLLHTFSVVPLLNRFS
Sbjct: 541 VLRASAAKVISGEHASPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFSVVPLLNRFS 600
Query: 601 IKTQCLLDGQKTGTVLSASALLLNESCGSSLPPTLANVAVSTSSIGSMMGGVVGGDSGWK 660
KTQCLLDGQKTGTVLSASALLLNE GSSLPPTL+NVAVSTSSIGSMMGGVVGGDSGWK
Sbjct: 601 SKTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGGVVGGDSGWK 660
Query: 661 LFNEGSSLVEEGVVIFATHQTALVVRLSPTLEVYAQLSKPDGVREGSMPYTAWKCSQSFE 720
LFNEGSSLVEEGVVIFATHQTALVVRLSPT+EVYAQLSKPDG+REGSMPYTAWKCSQSFE
Sbjct: 661 LFNEGSSLVEEGVVIFATHQTALVVRLSPTVEVYAQLSKPDGIREGSMPYTAWKCSQSFE 720
Query: 721 TSPSEAVERVSLLAIAWDKMVQVAKLVKAELKVCGKWSLESAAIGVAWLDDQVLVVLTIT 780
TSPSEAVERVSLLAIAWDKMVQVAKLVK ELKVCGKWSLESAAIGV WLDDQVLV+LT+T
Sbjct: 721 TSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVWLDDQVLVILTVT 780
Query: 781 GQLFLFEKDGTMIHQTSVFVDGFDKEDFIAYHTHFVNVLGNPEKAYHNCVAVRGASIYVL 840
GQLFLFEKDGTMIHQTS+FVDGF KEDFIAYHTHF N+LGNPEKAYHNCVAVRGASIYVL
Sbjct: 781 GQLFLFEKDGTMIHQTSIFVDGFVKEDFIAYHTHFANILGNPEKAYHNCVAVRGASIYVL 840
Query: 841 GPMHLIISRLLPWKERVQVLRKAGDWMGALSMAITIYDGQAHGVIDLPRSLESLQELVVP 900
GPMHL+ISRLLPWKERVQVLRKAGDWM ALSMAITIYDG AHGVIDLPRSLESLQELV+P
Sbjct: 841 GPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSLESLQELVMP 900
Query: 901 FLIELLLSYVDEVFSYISVAFCNQIEKNEKSVDVTSGGHSAHSEIKEQYNRVGGVAVEFC 960
FLIELLLSYVDEVFSYISVAFCNQIEKNEK D+T HSAHSEIKEQYNRVGGVAVEFC
Sbjct: 901 FLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQYNRVGGVAVEFC 960
Query: 961 VHIRRTDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKGWL 1020
VHI RTDILFDEIFSKFV VQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKGWL
Sbjct: 961 VHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKGWL 1020
Query: 1021 QRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLQTSKS 1080
QRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVL+TSKS
Sbjct: 1021 QRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLRTSKS 1080
Query: 1081 ERASAIGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQPLRDELLQFLLENSDSVDTRSISN 1140
+ AS++GYKTLVYLKYCFSGLAFPPGQGTLAHSRVQ LRDELLQFLLENSD+VDTRSISN
Sbjct: 1081 KHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSDAVDTRSISN 1140
Query: 1141 KSSEIGYLKLYHLLELDTGATLDVLRCAFVEGEIPKTNSSPNGLDDVSMQVQEEKNSTSG 1200
KSSE+G L LY LLELDT ATLDVLRCAFVEGEI K SS +G D SMQ+QEEKNS SG
Sbjct: 1141 KSSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTSMQLQEEKNSISG 1200
Query: 1201 RKNVLIQSIVDALVHVLDQAICQTDRSAGGEDIALVGDWPSKKDLVHLFDFVANYVACGR 1260
RKN LIQ++VDALVHVLD+AIC+TD S G++I LV DWPSKK+L+HLFDF+A YVACG+
Sbjct: 1201 RKNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHLFDFIATYVACGK 1260
Query: 1261 ATASKVVVGQILEHLISNSDIPETENDFLPKVTANSVHSRKREKQVLSLLEVVPETHWNA 1320
AT SK VVGQILEHLISNSDIPET +DFLP+VTANSV SRKREKQVLSLLEV+PETHWN
Sbjct: 1261 ATVSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLSLLEVIPETHWNP 1320
Query: 1321 SSVLSMCEEAQFFQVCGLIHSIRHQYASALDSYMKDIDEPIHGFAFINRTLLELNNYERT 1380
SSVL MCE+AQFFQVCGLIHSI HQY+SALDSYMKD+DEPIH F FINRTLLEL N E+T
Sbjct: 1321 SSVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFINRTLLELGNSEQT 1380
Query: 1381 EFQAVVISRIPELFNLNREGTFFLVIDHFNNDASDILSQLRTHPRSLFLYLKTLIEVHLS 1440
EF+AVVISRIPELFNLNR TFFLVIDHFNND S+ILSQLR HPRSLFLYLKTLIEVHLS
Sbjct: 1381 EFRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLFLYLKTLIEVHLS 1440
Query: 1441 GSLNFSCLRKDDIFGLNYSTKGLDDYLRKLSDFPKYLSNNPVDVTDDIIELYVELLCQYE 1500
GS +FSCL+KDD G+NYSTKG+DDYL+KLSDFPKYLSNNPVDVTDDIIELYVELLCQ+E
Sbjct: 1441 GSPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDDIIELYVELLCQHE 1500
Query: 1501 RESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDKKFNDL 1560
RESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDKKF+DL
Sbjct: 1501 RESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDKKFHDL 1560
Query: 1561 EAAVESIVANGASSGSNDPRHFNSVLKLQEVNDIDVLLHACIGLCQRNTPRLNSEESETL 1620
EAAV + V+N ASSGSND ++FNSVLKLQEVN + VLLHACIGLCQRNTPRLNSEES+TL
Sbjct: 1561 EAAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRNTPRLNSEESQTL 1620
Query: 1621 WFKLLDSFCEPLIDSYNYRTPAFGENQVQFLNESSGLQKDKEARIVTWRILKSDKAAHIL 1680
WFKLLDSFCEPLIDSYN+RT +F +NQVQFLNESS QKDKEA IVTWRILKS+K AH+L
Sbjct: 1621 WFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTWRILKSNKVAHLL 1680
Query: 1681 KRLFSRFIKEIVEGMIGYVHLPIIMSRLLSDNGSQEFGDFKLTVLGMLGTFGFERRILDT 1740
++LFS+FI+EIVEGM+GYVHLP IMSRLL DNGSQEFGDFKLT+LGMLGTFGFERRILD+
Sbjct: 1681 RKLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTFGFERRILDS 1740
Query: 1741 AKALIEDDTFYTMSLLKKGASHGYAPRNLVCCICNCLLIKSSSSYRVRVFNCGHATHLQC 1800
AKALIEDD+FYTMSLLKKGA+HGYAPR++VCCICN LL+KSSSSYRVRVFNCGHATHLQC
Sbjct: 1741 AKALIEDDSFYTMSLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFNCGHATHLQC 1800
Query: 1801 EVLDNEASGGDFSCPVCIHSNQSQRSRGKALTEYSLVNTFSSGTQSLSGASVQYPQETDL 1860
E L+NEASGGD++CP+C+HSNQSQ S+ KA TEYSLVN FSS TQS SGASV YPQETDL
Sbjct: 1801 EDLENEASGGDYTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTQSSSGASVSYPQETDL 1860
Query: 1861 LELPYTLQQIPRFEILTNLQKNQRVIEIENVPQLRLAPPAVYHDKVTKGYHLLVGESSSG 1920
LELPYTLQQIPRFEILTNLQKNQRVI+IENVPQLRLAPPAVYHDKVTKGYHLLVGESS G
Sbjct: 1861 LELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYHLLVGESSGG 1920
Query: 1921 ---IEKQNKSKQLREVKVKRRSSLRFPLKASLFGE 1948
+EK NKS+QL VKVKR SSLRFPLK SLFG+
Sbjct: 1921 REKVEKLNKSRQLTGVKVKRPSSLRFPLKTSLFGK 1954
BLAST of MS010323 vs. ExPASy TrEMBL
Match:
A0A1S3AUY3 (vacuolar protein sorting-associated protein 8 homolog OS=Cucumis melo OX=3656 GN=LOC103483112 PE=3 SV=1)
HSP 1 Score: 3189.8 bits (8269), Expect = 0.0e+00
Identity = 1639/1961 (83.58%), Postives = 1766/1961 (90.06%), Query Frame = 0
Query: 1 MTKELTDTGVLPLMELDLNAFIHAHLSSSDDDDDDDPSFPHRTIDEILNES----GSAPS 60
MT+ELTDT LP MELDLNAFIHAHLSS DDDDDDD SFPHR+IDEILN+S S+PS
Sbjct: 1 MTEELTDTRTLPPMELDLNAFIHAHLSSGDDDDDDDLSFPHRSIDEILNDSSSSTSSSPS 60
Query: 61 SPPHSPPYSGDRAVATRDGAVSESPSTPPSKS-------PFEAPRSNQWNEKSVQLRPAA 120
S PHSPP G R + +G VS SPST P KS EAPR+N WNEKS Q +P
Sbjct: 61 SSPHSPPSRGRRNIVAGNGGVSASPSTSPFKSLLEETIKDSEAPRNNPWNEKSAQSKPGK 120
Query: 121 VSLVKVGELTDDLFRRGSRPLPSLFGGVRSNAKPGAALAAAAAASRSMPAPHAAAIKSRR 180
VS K+GELTDD FRRGSRPLPSLFG VRSNAKPGAALAAAAAASRS PAPHAAAIKSRR
Sbjct: 121 VSHSKIGELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIKSRR 180
Query: 181 AGHGSMVIDDDELASPSADDSEAVFGGLYSTNTHSEESGENSTVMDDTDADYQVASINVG 240
AG+G+M +DDDELAS SA DSE + LY TN H +ESGENS + D DYQVAS +V
Sbjct: 181 AGYGNMALDDDELASSSAVDSEFLSDSLYHTNIHLKESGENSISVVDRITDYQVASRDV- 240
Query: 241 GGVWARDRIRDDVPDNDEFRITKGLKCVAEQRSVDDVNFDESTTILPPVEANDGSVSDYA 300
+W R+ IRD VP NDEFR+T+ ++ AE SVDDVNF+ES T +PP E ND S+ A
Sbjct: 241 SELWDRNNIRDSVPHNDEFRMTEDMEFEAEPSSVDDVNFNESLTTVPPAETNDRSLLGPA 300
Query: 301 DKNVCSTELRPSILDVDESNEGAIPSSSEPDNDGSAVDYGSLEWETQDFKKCLQQSKDTE 360
+KNVCST+ P+ LDVDESNEGAIP S+EPD++GSAV YGS E ETQDF+K Q SKDTE
Sbjct: 301 EKNVCSTDAHPTELDVDESNEGAIPRSTEPDDEGSAVGYGSPELETQDFEKYHQPSKDTE 360
Query: 361 VDLVIKGPSVVNDISELGETTEHMDDSTISEQAEMMLLPSTNPLEAAEEIEKKQAFTALH 420
VDL I+ PS+VNDI E GETTE +D+ I + E M + STNPLE AEEIEKKQAFTALH
Sbjct: 361 VDLAIEDPSIVNDIIESGETTEQLDNLQIGKHPETMPVSSTNPLELAEEIEKKQAFTALH 420
Query: 421 WEEGVAAQPMKLEGIKGGSTTLGYFDIQADNSISRTISSHSFKREHGFPQVLAVHANYIA 480
WEEGVAAQPM+LEGIKG +TTLGYFDIQADNSISRTISSHSF+REHGFPQVLAVHANYIA
Sbjct: 421 WEEGVAAQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHANYIA 480
Query: 481 VGMSKGNIVVVASKYSAQNGDNMDAKMLLLGLQGDKSTAPVTSLCFNLQGDLLLAGYNDG 540
VGMSKG+IVVVASKYSAQNGDNMDAKM+LLG QGDKSTAPVTSLCF+ Q DLLLAGY+DG
Sbjct: 481 VGMSKGSIVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPVTSLCFSQQADLLLAGYSDG 540
Query: 541 HITVWDVLRASAAKIISGEHTSPVVHSLFLGQDAQVTRQFKAVTGDSKGLVLLHTFSVVP 600
HITVWDVLRASAAK+ISGEHTSPVVHSLFLGQ+AQVTRQFKAVTGDSKGLVLLHTFSVVP
Sbjct: 541 HITVWDVLRASAAKVISGEHTSPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFSVVP 600
Query: 601 LLNRFSIKTQCLLDGQKTGTVLSASALLLNESCGSSLPPTLANVAVSTSSIGSMMGGVVG 660
LLNRFSIKTQCLLDGQKTGTVLSASALLLNE GSSLPPTL+NVAVSTSSIGSMMGGVVG
Sbjct: 601 LLNRFSIKTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGGVVG 660
Query: 661 GDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSPTLEVYAQLSKPDGVREGSMPYTAWK 720
GDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSP++EVYAQLSKPDG+REGSMPYTAWK
Sbjct: 661 GDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSPSVEVYAQLSKPDGIREGSMPYTAWK 720
Query: 721 CSQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKAELKVCGKWSLESAAIGVAWLDDQVL 780
CSQSFETS SEAVERVSLLAIAWDKMVQVAKLVK ELKVCG WSLESAAIGV WLDDQVL
Sbjct: 721 CSQSFETSSSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGNWSLESAAIGVVWLDDQVL 780
Query: 781 VVLTITGQLFLFEKDGTMIHQTSVFVDGFDKEDFIAYHTHFVNVLGNPEKAYHNCVAVRG 840
V+LT+TGQLFLFEKDGTMIHQTSVF DGF KEDFIAYHTHF NVLG+PEKAYHNCVAVRG
Sbjct: 781 VILTVTGQLFLFEKDGTMIHQTSVFADGFVKEDFIAYHTHFANVLGHPEKAYHNCVAVRG 840
Query: 841 ASIYVLGPMHLIISRLLPWKERVQVLRKAGDWMGALSMAITIYDGQAHGVIDLPRSLESL 900
ASIYVLGP HL+ISRLLPWKERVQVLRKAGDWM ALSMAITIYDG AHGVIDLPRSLESL
Sbjct: 841 ASIYVLGPTHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSLESL 900
Query: 901 QELVVPFLIELLLSYVDEVFSYISVAFCNQIEKNEKSVDVTSGGHSAHSEIKEQYNRVGG 960
QELV+PFLIELLLSYVDEVFSYISVAFCNQIEKNEK DVTS SAHSEIKEQYNRVGG
Sbjct: 901 QELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDVTSERDSAHSEIKEQYNRVGG 960
Query: 961 VAVEFCVHIRRTDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHY 1020
VAVEFCVHI RTDILFDEIFSKFV VQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHY
Sbjct: 961 VAVEFCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHY 1020
Query: 1021 SHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAV 1080
SHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAV
Sbjct: 1021 SHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAV 1080
Query: 1081 LQTSKSERASAIGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQPLRDELLQFLLENSDSVD 1140
L+TSKS+ AS++GYKTLVYLKYCFSGLAFPPGQGTLAHSRVQ LRDELLQFLLENSD+VD
Sbjct: 1081 LRTSKSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSDAVD 1140
Query: 1141 TRSISNKSSEIGYLKLYHLLELDTGATLDVLRCAFVEGEIPKTNSSPNGLDDVSMQVQEE 1200
TRSISNKSSE+G L LYHLLELDT ATLDVLRCAFVE E KTNSS +G D M++Q+E
Sbjct: 1141 TRSISNKSSEVGCLNLYHLLELDTEATLDVLRCAFVEVEFLKTNSSLDGPVDAIMELQDE 1200
Query: 1201 KNSTSGRKNVLIQSIVDALVHVLDQAICQTDRSAGGEDIALVGDWPSKKDLVHLFDFVAN 1260
KNS SGRKN LIQ++VDALVHVL +AIC+TD S G++I LV DWPSKK+L+HLFDF+A
Sbjct: 1201 KNSISGRKNFLIQNVVDALVHVLGKAICETDESPDGDNITLVDDWPSKKELIHLFDFIAT 1260
Query: 1261 YVACGRATASKVVVGQILEHLISNSDIPETENDFLPKVTANSVHSRKREKQVLSLLEVVP 1320
YVACG+AT SK VVGQILEHLISN+ IPET +DFLP+VTANSVHSRKREKQVLSLLEVVP
Sbjct: 1261 YVACGKATVSKDVVGQILEHLISNTHIPET-SDFLPRVTANSVHSRKREKQVLSLLEVVP 1320
Query: 1321 ETHWNASSVLSMCEEAQFFQVCGLIHSIRHQYASALDSYMKDIDEPIHGFAFINRTLLEL 1380
ETHWN SSVL MCE+AQFFQVCGLIHSI QY+SALDSYMKD+ EPIH FAFINR LL+L
Sbjct: 1321 ETHWNPSSVLRMCEKAQFFQVCGLIHSIGCQYSSALDSYMKDVGEPIHAFAFINRALLKL 1380
Query: 1381 NNYERTEFQAVVISRIPELFNLNREGTFFLVIDHFNNDASDILSQLRTHPRSLFLYLKTL 1440
+N E+TEF+AVVISRIPELFNLNR TFFLVIDHFN+D S+IL QLR HPRSLFLYLKTL
Sbjct: 1381 SNSEQTEFRAVVISRIPELFNLNRGATFFLVIDHFNDDVSNILLQLRNHPRSLFLYLKTL 1440
Query: 1441 IEVHLSGSLNFSCLRKDDIFGLNYSTKGLDDYLRKLSDFPKYLSNNPVDVTDDIIELYVE 1500
IEVHLSGSL+FSCL+KDD G+NYSTKGLDDYL+KLSDFPKYLSNNPVDVTDDIIELYVE
Sbjct: 1441 IEVHLSGSLDFSCLKKDDNLGVNYSTKGLDDYLKKLSDFPKYLSNNPVDVTDDIIELYVE 1500
Query: 1501 LLCQYERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLD 1560
LLCQ+ERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLD
Sbjct: 1501 LLCQHERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLD 1560
Query: 1561 KKFNDLEAAVESIVANGASSGSNDPRHFNSVLKLQEVNDIDVLLHACIGLCQRNTPRLNS 1620
KKF+DLEAAV +IV+NGASSGS+D +HF+SVLKLQEVN ++VLLHACIGLCQRNTPRLN
Sbjct: 1561 KKFHDLEAAVGAIVSNGASSGSSDSQHFDSVLKLQEVNTVEVLLHACIGLCQRNTPRLNC 1620
Query: 1621 EESETLWFKLLDSFCEPLIDSYNYRTPAFGENQVQFLNESSGLQKDKEARIVTWRILKSD 1680
EESETLWFKLLDSFCEPLIDSYN+RT +F +NQVQFLNE S QKDKEA IVTWRILKS+
Sbjct: 1621 EESETLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNEPSSSQKDKEANIVTWRILKSN 1680
Query: 1681 KAAHILKRLFSRFIKEIVEGMIGYVHLPIIMSRLLSDNGSQEFGDFKLTVLGMLGTFGFE 1740
KAAHIL++LFS+FI+EIVEGM+GYVHLP IMSRLL DNGSQEFGDFKLT+LGMLGTFGFE
Sbjct: 1681 KAAHILRKLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTFGFE 1740
Query: 1741 RRILDTAKALIEDDTFYTMSLLKKGASHGYAPRNLVCCICNCLLIKSSSSYRVRVFNCGH 1800
RRILDTAKALIEDD+FYTM+LLKKGA+HGYAPR++VCCICN LL+KSSSSYRVRVFNCGH
Sbjct: 1741 RRILDTAKALIEDDSFYTMNLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFNCGH 1800
Query: 1801 ATHLQCEVLDNEASGGDFSCPVCIHSNQSQRSRGKALTEYSLVNTFSSGTQSLSGASVQY 1860
ATHLQCE L+NEASGGD +CP+C+HSNQSQ S+ KA TEYSLVN FSS T S SGASV Y
Sbjct: 1801 ATHLQCEDLENEASGGDSTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTSSSSGASVSY 1860
Query: 1861 PQETDLLELPYTLQQIPRFEILTNLQKNQRVIEIENVPQLRLAPPAVYHDKVTKGYHLLV 1920
PQETD+LELPYTLQQIPRFEILTNLQKNQRVI+IENVPQLRLAPPAVYHDKVTKGYHLLV
Sbjct: 1861 PQETDILELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYHLLV 1920
Query: 1921 GESSSG---IEKQNKSKQLREVKVKRRSSLRFPLKASLFGE 1948
GESSSG +EK NKS+QL EVKVKR SSLRFPLKASLFG+
Sbjct: 1921 GESSSGREKVEKLNKSRQLTEVKVKRPSSLRFPLKASLFGK 1959
BLAST of MS010323 vs. TAIR 10
Match:
AT4G00800.1 (transducin family protein / WD-40 repeat family protein )
HSP 1 Score: 1895.6 bits (4909), Expect = 0.0e+00
Identity = 1079/1964 (54.94%), Postives = 1370/1964 (69.76%), Query Frame = 0
Query: 14 MELDLNAFIHAHLSSSDDDDD-DDPSFPHRTIDEILN--ESGSAPSSPPHSPPYSGDRAV 73
MELDL++F+ +S SD D D D S PHRT+DEILN S SA SSPP SPP R
Sbjct: 1 MELDLDSFL---VSDSDSDSDLDSSSVPHRTVDEILNASSSSSASSSPPSSPPSINRRKQ 60
Query: 74 ATRDGAVSESPST-----PPSKSPFEAPRSNQWNEKSVQLRPAAVSLVKVGELTDDLFRR 133
+ +SE+ + P S+ P + + + S LR
Sbjct: 61 DDPNRRLSEALTNVAVLRPESELHRGFPPTRRNSTSSSSLR------------------- 120
Query: 134 GSRPLPSLFGGVRSNAKPGAALAAAAAASRSMPAPHAAAIKSRRAGHGSMVI-------- 193
PLPSL GVRSN KPGAALAAA AASR +P PHAA IKSRRA S +
Sbjct: 121 -QLPLPSLLAGVRSNVKPGAALAAAVAASRLVPTPHAAIIKSRRASSASSELLLQVSNQE 180
Query: 194 DDDELASPSADDSEAVFGGLYSTNTHSEESGENSTVMDDTDADYQVASINVGGGVWARDR 253
+DD S DS V G S + GE S + D+ + VAS+ V
Sbjct: 181 EDDHEVLSSNGDSVGVAAGSVSADDFRSFGGE-SLLEDEDNGVSGVASLEDEAKVM---- 240
Query: 254 IRDDVPDNDEFRITKGLK--CVAEQRSVD-DVNFDESTTILPPVEANDGSVSDYADKNVC 313
+V +D IT+ L V D + N +EA + ++ D D+
Sbjct: 241 ---EVQASD---ITESLNPDLVTVSSGFDSEGNVSTEKEAETTMEAGNAAIDDDTDE--- 300
Query: 314 STELRPSILDVDES-----NEGAIPSSSEPDNDGSAVDYGSLEWETQDFKKCLQQSKDTE 373
T L S+++ ES +EG + +++ S+V G ++ + D + +SK
Sbjct: 301 -TMLVASLVESSESQHLTDSEGKCDDAKVSNDEESSV--GDVKSDKSDI--IIPESKKEG 360
Query: 374 VDLVIKGP-SVVNDISEL-GETTEHMDDSTISEQAEMMLLPSTNPLEAAEEIEKKQAFTA 433
D I S ++ ISEL E +++ +S++ + L AEE EKKQA+T
Sbjct: 361 GDAFIPDDGSSMSGISELVEERIAELENERMSKRERLKSQSFRKQLVLAEEFEKKQAYTG 420
Query: 434 LHWEEGVAAQPMKLEGIKGGSTTLGYFDIQADNSISRTISSHSFKREHGFPQVLAVHANY 493
LHWEEG AAQPM+LEG+K GST LGYFD+ ADN ISRTISS +FKR+HG PQVLAVH NY
Sbjct: 421 LHWEEGAAAQPMRLEGVKIGSTNLGYFDVDADNVISRTISSQAFKRDHGSPQVLAVHLNY 480
Query: 494 IAVGMSKGNIVVVASKYSAQNGDNMDAKMLLLGLQGDKSTAPVTSLCFNLQGDLLLAGYN 553
IAVG SKG IVVV SKYS+ + D M++KM+ LGLQG++S +PVTS+CFN G LLLAGY
Sbjct: 481 IAVGTSKGVIVVVPSKYSSDHADQMESKMIWLGLQGERSQSPVTSVCFNQIGSLLLAGYG 540
Query: 554 DGHITVWDVLRASAAKIISGEHTSPVVHSLFLGQDAQVTRQFKAVTGDSKGLVLLHTFSV 613
DGH+TVWD+ RAS AK+I+ EHT+PVV++ FLG+D+Q +RQFK +T D+KG+V H+FS
Sbjct: 541 DGHVTVWDMQRASIAKVIT-EHTAPVVYAFFLGRDSQGSRQFKVITSDTKGVVFKHSFSY 600
Query: 614 VPLLNRFSIKTQCLLDGQKTGTVLSASALLLNESCGSSLPPTL-ANVAVSTSSIGSMMGG 673
LLN ++++TQCLLDGQK GTVLSAS L +E+ GSSL + N AV +SSI SMMGG
Sbjct: 601 ARLLNMYTVETQCLLDGQKNGTVLSASP-LPDENFGSSLVSSKGGNSAVPSSSISSMMGG 660
Query: 674 VVGGDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSPTLEVYAQLSKPDGVREGSMPYT 733
VVG S WKLFNE S+ VEEGVVIFAT+QT LVV+L P LEVYAQL +P+GVREGSMPYT
Sbjct: 661 VVGVGSTWKLFNEDSTSVEEGVVIFATYQTGLVVKLIPNLEVYAQLPRPEGVREGSMPYT 720
Query: 734 AWKCSQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKAELKVCGKWSLESAAIGVAWLDD 793
AW+ +S E EA +RVS L IAWD+ VQVAKLVK+++K KWSL+S AIGV WLDD
Sbjct: 721 AWR--RSTENYSKEAEDRVSFLVIAWDRRVQVAKLVKSDIKEYAKWSLDSPAIGVVWLDD 780
Query: 794 QVLVVLTITGQLFLFEKDGTMIHQTSVFVDGFDKEDFIAYHTHFVNVLGNPEKAYHNCVA 853
Q+LV+ T+TG L+LF +DG +IHQT+ V G D I+YHT+F NV GNPEKAYHN +
Sbjct: 781 QLLVIPTVTGHLYLFTRDGVVIHQTNFSVAGSSGNDLISYHTYFTNVFGNPEKAYHNSMG 840
Query: 854 VRGASIYVLGPMHLIISRLLPWKERVQVLRKAGDWMGALSMAITIYDGQAHGVIDLPRSL 913
VRGAS+Y+LG HL+ISRLLPWKERV VLR+ GDWMGA +MA+++++GQAHGV+DLP+++
Sbjct: 841 VRGASVYILGTAHLVISRLLPWKERVDVLRRGGDWMGAFNMAMSLFNGQAHGVVDLPKTV 900
Query: 914 ESLQELVVPFLIELLLSYVDEVFSYISVAFCNQIEKNEKSVDVTSGGHSAHSEIKEQYNR 973
++++E + P L ELLLSYVDEVFSYIS+AF NQIE N + + +SG ++ + EI+EQYNR
Sbjct: 901 DAIREAIAPSLAELLLSYVDEVFSYISIAFSNQIENNGVTHEPSSGINNVNLEIEEQYNR 960
Query: 974 VGGVAVEFCVHIRRTDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQALV 1033
VGGVAVEFCVHI R D+LFDEIFS+FVAVQQRDTFLELLEPYIL+DMLGSLPPEIMQALV
Sbjct: 961 VGGVAVEFCVHINRMDLLFDEIFSRFVAVQQRDTFLELLEPYILRDMLGSLPPEIMQALV 1020
Query: 1034 EHYSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL 1093
EHYS KGWLQR+EQCVLHMDISSLDFNQVVR+CR+HGLY AL+YLFNKGLDDFR+PLEEL
Sbjct: 1021 EHYSRKGWLQRIEQCVLHMDISSLDFNQVVRICREHGLYGALLYLFNKGLDDFRSPLEEL 1080
Query: 1094 LAVLQTSKSERASAIGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQPLRDELLQFLLENSD 1153
L VL+ S+ +RA+AIGY+ LVYLKYCF GLAFPPG GTL +R LR EL+QFLLE S+
Sbjct: 1081 LIVLRNSEKQRATAIGYRMLVYLKYCFLGLAFPPGHGTLNPTRWPSLRSELIQFLLEKSN 1140
Query: 1154 SVDTRSISNKSSEIGYLKLYHLLELDTGATLDVLRCAFVEGEIPKTNSSPNGLDDVSMQV 1213
+ D S + +S + YL LYHLLE+DT ATLDVLR AFVE E+ K S +VS++
Sbjct: 1141 AHD--SSTCVTSRLNYLNLYHLLEMDTEATLDVLRYAFVENEMVKHESHLLEYGEVSVES 1200
Query: 1214 QEEKNSTSGRKNVLIQSIVDALVHVLDQAICQTDRSAGGEDIALVGDWPSKKDLVHLFDF 1273
+ + + ++LIQ++VDALVHV D + ++ S D +WPSK+D HLF+F
Sbjct: 1201 KTDGSLPEVSNDILIQNLVDALVHVPDWGV--SNESGDPIDSKSDKNWPSKEDTSHLFEF 1260
Query: 1274 VANYVACGRATASKVVVGQILEHLISNSDIPETENDFLPKVTANSVHSRKREKQVLSLLE 1333
VA Y A GR + SK V+ QIL++L S+ +P +V S+ RE Q+L+LL+
Sbjct: 1261 VAYYAARGRVSISKSVLAQILDYLTSDHILP-----------TYNVSSKMRENQLLNLLK 1320
Query: 1334 VVPETHWNASSVLSMCEEAQFFQVCGLIHSIRHQYASALDSYMKDIDEPIHGFAFINRTL 1393
VPET W+A V +CE+A F+QVCG IH I +Y +ALDSY+K+ DEPIH F ++N+ L
Sbjct: 1321 AVPETDWDADYVSQLCEKAHFYQVCGYIHIIDRRYVAALDSYVKEADEPIHLFCYVNKML 1380
Query: 1394 LELNNYERTEFQAVVISRIPELFNLNREGTFFLVIDHFNNDASDILSQLRTHPRSLFLYL 1453
+L+ E T FQ+ +ISRIPEL +L+R+G FFL+I + + I QL +HPRSLFLYL
Sbjct: 1381 SQLSGDEFTAFQSAIISRIPELLDLSRQGAFFLIICNLKDTIKRIQEQLHSHPRSLFLYL 1440
Query: 1454 KTLIEVHLSGSLNFSCLRKD---DIFGLNYS---TKGLDDYLRKLSDFPKYLSNNPVDVT 1513
KT+IEV+LSGSL+FS LRK D G N K YL L+DFPK++ +NPV+VT
Sbjct: 1441 KTVIEVYLSGSLDFSRLRKHEAVDSSGENIRRDIPKEAKIYLEGLNDFPKFIQDNPVNVT 1500
Query: 1514 DDIIELYVELLCQYERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSA 1573
DD+IELYVELLC+YE +SVLKFLETFDSYRVEHCLRLCQ+Y ++DAAAFLLERVGD GSA
Sbjct: 1501 DDMIELYVELLCKYEPKSVLKFLETFDSYRVEHCLRLCQEYGIVDAAAFLLERVGDAGSA 1560
Query: 1574 LFLTLSSLDKKFNDLEAAVESIVAN---GASSGSNDPRHFNSVLKLQEVNDIDVLLHACI 1633
L LTLS L++K+ +LE AVE +++ GAS G++ HF+S L+L+EV+DI +L ACI
Sbjct: 1561 LSLTLSGLNEKYVELEIAVECLMSEMKLGASEGAS-LEHFSSALELKEVHDIQGVLQACI 1620
Query: 1634 GLCQRNTPRLNSEESETLWFKLLDSFCEPLIDSYNYRTPAFGENQVQFLNESSGLQKDKE 1693
GLCQRNTPRLN EESE LWF+ LD+FCEPL++SY G N+ +S ++
Sbjct: 1621 GLCQRNTPRLNPEESEILWFRFLDTFCEPLMESYREPKNTDGINKGSLGVKSLERHVNES 1680
Query: 1694 ARIVTWRILKSDKAA-HILKRLFSRFIKEIVEGMIGYVHLPIIMSRLLSDNGSQEFGDFK 1753
+ WRI +SD AA HIL++L S+FIKEIVEGMIGYV LP IM++LLSDNG+QEFGDFK
Sbjct: 1681 DVAIKWRIPRSDTAATHILRKLISQFIKEIVEGMIGYVRLPTIMTKLLSDNGTQEFGDFK 1740
Query: 1754 LTVLGMLGTFGFERRILDTAKALIEDDTFYTMSLLKKGASHGYAPRNLVCCICNCLLIKS 1813
LT+LGMLGT+GFERRILDTAK+LIEDDTFY+M+LLKKGASHGYAPR+L+CCIC+C L K+
Sbjct: 1741 LTILGMLGTYGFERRILDTAKSLIEDDTFYSMNLLKKGASHGYAPRSLLCCICSCPLTKT 1800
Query: 1814 SSSYRVRVFNCGHATHLQCEVLDNEASGGDFS-------CPVCIHSNQSQRS-RGKAL-T 1873
S+ RVRVFNCGHATHLQCE +NE S S CPVC+ S+ S +GK+
Sbjct: 1801 FSALRVRVFNCGHATHLQCEPSENETSTSASSIHVSSSGCPVCMTKKTSKSSLKGKSFYR 1860
Query: 1874 EYSLVNTFSSGTQSLSGASVQYPQETDLLELPYTLQQIPRFEILTNLQKNQRVIEIENVP 1932
+Y L++T SS S AS Y E ++ + + QQ+ RFEILTNLQK+QR+++IE++P
Sbjct: 1861 DYGLISTVSSNAGSSQRAS-PYSHENEMSDHSHN-QQLSRFEILTNLQKDQRLVQIESLP 1900
BLAST of MS010323 vs. TAIR 10
Match:
AT1G08190.1 (vacuolar protein sorting 41 )
HSP 1 Score: 45.8 bits (107), Expect = 4.5e-04
Identity = 25/85 (29.41%), Postives = 45/85 (52.94%), Query Frame = 0
Query: 1478 DVTDDIIELYVELLCQYERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDV 1537
D D ++ VEL +Y+ + +L FL + Y++E LC + + + F+L R+G+
Sbjct: 664 DTGKDFHDMQVELYAEYDTKMLLPFLRSSQHYKLEKAYELCVKKDFLREQVFVLGRMGNA 723
Query: 1538 GSALFLTLSSLDKKFNDLEAAVESI 1563
AL + ++ K D+E AVE +
Sbjct: 724 KQALAVIIN----KLGDIEEAVEFV 744
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_022144189.1 | 0.0e+00 | 92.40 | vacuolar protein sorting-associated protein 8 homolog [Momordica charantia] | [more] |
| XP_038883953.1 | 0.0e+00 | 85.51 | vacuolar protein sorting-associated protein 8 homolog isoform X1 [Benincasa hisp... | [more] |
| KAG6582725.1 | 0.0e+00 | 85.54 | Vacuolar protein sorting-associated protein 8-like protein, partial [Cucurbita a... | [more] |
| XP_023526489.1 | 0.0e+00 | 85.54 | vacuolar protein sorting-associated protein 8 homolog isoform X1 [Cucurbita pepo... | [more] |
| XP_022924308.1 | 0.0e+00 | 85.34 | vacuolar protein sorting-associated protein 8 homolog isoform X1 [Cucurbita mosc... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q8N3P4 | 7.6e-89 | 23.66 | Vacuolar protein sorting-associated protein 8 homolog OS=Homo sapiens OX=9606 GN... | [more] |
| Q0P5W1 | 3.8e-88 | 23.69 | Vacuolar protein sorting-associated protein 8 homolog OS=Mus musculus OX=10090 G... | [more] |
| Q9VRX2 | 1.1e-26 | 22.87 | Vacuolar protein sorting-associated protein 8 homolog OS=Drosophila melanogaster... | [more] |
| P39702 | 5.9e-09 | 19.11 | Vacuolar protein sorting-associated protein 8 OS=Saccharomyces cerevisiae (strai... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1CRK3 | 0.0e+00 | 92.40 | vacuolar protein sorting-associated protein 8 homolog OS=Momordica charantia OX=... | [more] |
| A0A6J1EC21 | 0.0e+00 | 85.34 | vacuolar protein sorting-associated protein 8 homolog isoform X1 OS=Cucurbita mo... | [more] |
| A0A6J1IVQ0 | 0.0e+00 | 85.06 | vacuolar protein sorting-associated protein 8 homolog isoform X1 OS=Cucurbita ma... | [more] |
| A0A0A0L2X7 | 0.0e+00 | 83.99 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G116870 PE=3 SV=1 | [more] |
| A0A1S3AUY3 | 0.0e+00 | 83.58 | vacuolar protein sorting-associated protein 8 homolog OS=Cucumis melo OX=3656 GN... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT4G00800.1 | 0.0e+00 | 54.94 | transducin family protein / WD-40 repeat family protein | [more] |
| AT1G08190.1 | 4.5e-04 | 29.41 | vacuolar protein sorting 41 | [more] |
