MS007888 (gene) Bitter gourd (TR) v1

Overview


Name	MS007888
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	aspartic proteinase CDR1-like
Location	scaffold13: 2126809 .. 2128460 (-)
RNA-Seq Expression	MS007888
Synteny	MS007888

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TCCGCCGTCGTCGCCGTCGATTATGGCTTCACCGTCGAATTGATCCACCGAGATTCTCCCAATTCGCCGCTGTTCAACCCCTCGGAGACCCACTACCACCGCCTCACTAATGCCCTCCGCCGCTCCATCAGCCGGCACGCGGCGGCGCCGGCGGCGGACGTTGCGGAGGCGCCGATGTATAACAACAGAGGCGAGTATCTCATGAAGCTATCCCTCGGAACGCCGCCGTTTCCGATCATGGCCATTGCCGATACCGGAAGCGACATCGTTTGGAGCCAGTGTGCGCCGTGCACTCGGTGCTACACGCAGTCAGCGCCGATGTTTGACCCGAGTAAATCGGCGACTTATAAACAAATCCTCTGCTCCTCGCCGATTTGCCCGTTCTCCGGAACCGACTCTTGCCCGGTTCGGTCTGACCAGTGTGAGTACTCGATTAATTACGGCGATGGGTCCCACAGCCGCGGCGACATTGCCGTCGACACCGTGACGCTGGGGTCCACCTCCGGCCGGCCGGTGGCTTTTCCTCGGACCGTGTTCGGCTGCGGCCGTGACAGTGCCGGAACCTTTAATGCGAATGTCTCCGGCATAGTGGGGCTCGGCCACGGCGCCGTGTCGCTGGTCTCGCAGATGGGGGCGGCCACCGCCGGGAAATTCTCTTACTGTTTAGCTCCGATCGGTTCCGATTCGATCCGTTCCAGCAAGCTCAGCTTCGGCGCTAATGCTGTTGTCGCCGGCGCCGGAACTGTCTCCACCCCTATTTATACAGGTAGTGATGATAAATTATTACTAAATAATGAATCGAAATAATTAACAATTTTTTTTAAGGTGCTTTAATATTCAAACAAATACTAAAAATTATGCTAATTTTATCATATATTTTTTTTTCTATATTTTTCACTTTTCTTCGAACTATATTTACATTTTTAACCAATTATTAAAAACAAAATTTCCTTTTTGAAAAATATGTTTCAAATTTTGGCTTTAATTTTTAAAACAATTGGAAATAATTGATAATAACAAGATAAAGAATACATAGCAATAATGAAAGCTCGACTTTCGATGACAGAAAATTAAAAATGATTAAATACTTGTCAAATAGGAAATTTTTATTTATGCTCACTGTCAATAAAAATTTTATCAATATATTTAACTTTTCATTCATATTCTTGTGAGAAGACGATGGTCCGTTCTATCGGCTCAAGTTGGAAGCTATGAGCGTTGGGAATAACAAAATCAAGTTTTCGACCGCGTCTTCGTCACCCGGAGATGCAAACATCATCATCGACTCCGGCACCACCGTCACGTTACTCCCACCGGATTTCTACGCCGACTTTGGCACCGCAGTTTCCGACGGGATCAATCTCGAGCGCGTGAGAGACCCGAACCAATTTCTAGACTTCTGCTTCCAGTCGACGGACGACGACTTCGACGCGCCGTCCATCACGGTGCACTTCGAAGGCGGGGACGTGAAACTGAGCCGGGAAAATGTGTTCATAAGGATGGCGGACGACGTCGTTTGCTTGGCCTTCGTCAGCGCCCCCCAAAACGGCGGCGTTTCCATCTACGGCAATATTTTGCAGACCAACTTCTTGATCGGTTACGATACTCAGAAAATGGCTGTCTCCTTCAGGCCGGCGGATTGCGCCGCCATG

mRNA sequence

TCCGCCGTCGTCGCCGTCGATTATGGCTTCACCGTCGAATTGATCCACCGAGATTCTCCCAATTCGCCGCTGTTCAACCCCTCGGAGACCCACTACCACCGCCTCACTAATGCCCTCCGCCGCTCCATCAGCCGGCACGCGGCGGCGCCGGCGGCGGACGTTGCGGAGGCGCCGATGTATAACAACAGAGGCGAGTATCTCATGAAGCTATCCCTCGGAACGCCGCCGTTTCCGATCATGGCCATTGCCGATACCGGAAGCGACATCGTTTGGAGCCAGTGTGCGCCGTGCACTCGGTGCTACACGCAGTCAGCGCCGATGTTTGACCCGAGTAAATCGGCGACTTATAAACAAATCCTCTGCTCCTCGCCGATTTGCCCGTTCTCCGGAACCGACTCTTGCCCGGTTCGGTCTGACCAGTGTGAGTACTCGATTAATTACGGCGATGGGTCCCACAGCCGCGGCGACATTGCCGTCGACACCGTGACGCTGGGGTCCACCTCCGGCCGGCCGGTGGCTTTTCCTCGGACCGTGTTCGGCTGCGGCCGTGACAGTGCCGGAACCTTTAATGCGAATGTCTCCGGCATAGTGGGGCTCGGCCACGGCGCCGTGTCGCTGGTCTCGCAGATGGGGGCGGCCACCGCCGGGAAATTCTCTTACTGTTTAGCTCCGATCGGTTCCGATTCGATCCGTTCCAGCAAGCTCAGCTTCGGCGCTAATGCTGTTGTCGCCGGCGCCGGAACTGTCTCCACCCCTATTTATACAGACGATGGTCCGTTCTATCGGCTCAAGTTGGAAGCTATGAGCGTTGGGAATAACAAAATCAAGTTTTCGACCGCGTCTTCGTCACCCGGAGATGCAAACATCATCATCGACTCCGGCACCACCGTCACGTTACTCCCACCGGATTTCTACGCCGACTTTGGCACCGCAGTTTCCGACGGGATCAATCTCGAGCGCGTGAGAGACCCGAACCAATTTCTAGACTTCTGCTTCCAGTCGACGGACGACGACTTCGACGCGCCGTCCATCACGGTGCACTTCGAAGGCGGGGACGTGAAACTGAGCCGGGAAAATGTGTTCATAAGGATGGCGGACGACGTCGTTTGCTTGGCCTTCGTCAGCGCCCCCCAAAACGGCGGCGTTTCCATCTACGGCAATATTTTGCAGACCAACTTCTTGATCGGTTACGATACTCAGAAAATGGCTGTCTCCTTCAGGCCGGCGGATTGCGCCGCCATG

Coding sequence (CDS)

TCCGCCGTCGTCGCCGTCGATTATGGCTTCACCGTCGAATTGATCCACCGAGATTCTCCCAATTCGCCGCTGTTCAACCCCTCGGAGACCCACTACCACCGCCTCACTAATGCCCTCCGCCGCTCCATCAGCCGGCACGCGGCGGCGCCGGCGGCGGACGTTGCGGAGGCGCCGATGTATAACAACAGAGGCGAGTATCTCATGAAGCTATCCCTCGGAACGCCGCCGTTTCCGATCATGGCCATTGCCGATACCGGAAGCGACATCGTTTGGAGCCAGTGTGCGCCGTGCACTCGGTGCTACACGCAGTCAGCGCCGATGTTTGACCCGAGTAAATCGGCGACTTATAAACAAATCCTCTGCTCCTCGCCGATTTGCCCGTTCTCCGGAACCGACTCTTGCCCGGTTCGGTCTGACCAGTGTGAGTACTCGATTAATTACGGCGATGGGTCCCACAGCCGCGGCGACATTGCCGTCGACACCGTGACGCTGGGGTCCACCTCCGGCCGGCCGGTGGCTTTTCCTCGGACCGTGTTCGGCTGCGGCCGTGACAGTGCCGGAACCTTTAATGCGAATGTCTCCGGCATAGTGGGGCTCGGCCACGGCGCCGTGTCGCTGGTCTCGCAGATGGGGGCGGCCACCGCCGGGAAATTCTCTTACTGTTTAGCTCCGATCGGTTCCGATTCGATCCGTTCCAGCAAGCTCAGCTTCGGCGCTAATGCTGTTGTCGCCGGCGCCGGAACTGTCTCCACCCCTATTTATACAGACGATGGTCCGTTCTATCGGCTCAAGTTGGAAGCTATGAGCGTTGGGAATAACAAAATCAAGTTTTCGACCGCGTCTTCGTCACCCGGAGATGCAAACATCATCATCGACTCCGGCACCACCGTCACGTTACTCCCACCGGATTTCTACGCCGACTTTGGCACCGCAGTTTCCGACGGGATCAATCTCGAGCGCGTGAGAGACCCGAACCAATTTCTAGACTTCTGCTTCCAGTCGACGGACGACGACTTCGACGCGCCGTCCATCACGGTGCACTTCGAAGGCGGGGACGTGAAACTGAGCCGGGAAAATGTGTTCATAAGGATGGCGGACGACGTCGTTTGCTTGGCCTTCGTCAGCGCCCCCCAAAACGGCGGCGTTTCCATCTACGGCAATATTTTGCAGACCAACTTCTTGATCGGTTACGATACTCAGAAAATGGCTGTCTCCTTCAGGCCGGCGGATTGCGCCGCCATG

Protein sequence

SAVVAVDYGFTVELIHRDSPNSPLFNPSETHYHRLTNALRRSISRHAAAPAADVAEAPMYNNRGEYLMKLSLGTPPFPIMAIADTGSDIVWSQCAPCTRCYTQSAPMFDPSKSATYKQILCSSPICPFSGTDSCPVRSDQCEYSINYGDGSHSRGDIAVDTVTLGSTSGRPVAFPRTVFGCGRDSAGTFNANVSGIVGLGHGAVSLVSQMGAATAGKFSYCLAPIGSDSIRSSKLSFGANAVVAGAGTVSTPIYTDDGPFYRLKLEAMSVGNNKIKFSTASSSPGDANIIIDSGTTVTLLPPDFYADFGTAVSDGINLERVRDPNQFLDFCFQSTDDDFDAPSITVHFEGGDVKLSRENVFIRMADDVVCLAFVSAPQNGGVSIYGNILQTNFLIGYDTQKMAVSFRPADCAAM

Homology

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BLAST of MS007888 vs. NCBI nr
Match: XP_022155743.1 (aspartic proteinase CDR1-like [Momordica charantia])

HSP 1 Score: 819.7 bits (2116), Expect = 1.1e-233
Identity = 407/414 (98.31%), Postives = 407/414 (98.31%), Query Frame = 0


Query: 1   SAVVAVDYGFTVELIHRDSPNSPLFNPSETHYHRLTNALRRSISRHAAAPAADVAEAPMY 60
           SAVVAVDYGFTVELIHRDSPNSPLFNPSETHYHRLTNALRRSISRHAAAPAADVAEAPMY
Sbjct: 12  SAVVAVDYGFTVELIHRDSPNSPLFNPSETHYHRLTNALRRSISRHAAAPAADVAEAPMY 71

Query: 61  NNRGEYLMKLSLGTPPFPIMAIADTGSDIVWSQCAPCTRCYTQSAPMFDPSKSATYKQIL 120
           NNRGEYLMKLSLGTPPFPIMAIADTGSDIVWSQCAPCTRCYTQSAPMFDP KSATYKQIL
Sbjct: 72  NNRGEYLMKLSLGTPPFPIMAIADTGSDIVWSQCAPCTRCYTQSAPMFDPRKSATYKQIL 131

Query: 121 CSSPICPFSGTDSCPVRSDQCEYSINYGDGSHSRGDIAVDTVTLGSTSGRPVAFPRTVFG 180
           CSSPICPFSGTDSCPVRSDQCEYSINYGDGSHSRGDIAVDTVTLGSTSGRPVAFP TVFG
Sbjct: 132 CSSPICPFSGTDSCPVRSDQCEYSINYGDGSHSRGDIAVDTVTLGSTSGRPVAFPWTVFG 191

Query: 181 CGRDSAGTFNANVSGIVGLGHGAVSLVSQMGAATAGKFSYCLAPIGSDSIRSSKLSFGAN 240
           CGRDSAGTFNANVSGIVGLGHG VSLVSQMGAATAGKFSYCLA IGSDSIRSSKLSFGAN
Sbjct: 192 CGRDSAGTFNANVSGIVGLGHGVVSLVSQMGAATAGKFSYCLASIGSDSIRSSKLSFGAN 251

Query: 241 AVVAGAGTVSTPIYTDDGPFYRLKLEAMSVGNNKIKFSTASSSPGDANIIIDSGTTVTLL 300
           AVVAGAGTVSTPIYT DGPFYRLKLEAMSVGNNKI FSTASSSPGDANIIIDSGTTVTLL
Sbjct: 252 AVVAGAGTVSTPIYTADGPFYRLKLEAMSVGNNKITFSTASSSPGDANIIIDSGTTVTLL 311

Query: 301 PPDFYADFGTAVSDGINLERVRDPNQFLDFCFQSTDDDFDAPSITVHFEGGDVKLSRENV 360
           PPDFYADFGTAVSDGINLERVRDPNQFLDFCFQST DDFDAPSITVHFEGGDVKLSRENV
Sbjct: 312 PPDFYADFGTAVSDGINLERVRDPNQFLDFCFQSTADDFDAPSITVHFEGGDVKLSRENV 371

Query: 361 FIRMADDVVCLAFVSAPQNGGVSIYGNILQTNFLIGYDTQKMAVSFRPADCAAM 415
           FIRMADDVVCLAFVSAPQNGGVSIYGNILQTNFLIGYDTQKMAVSFRPADCAAM
Sbjct: 372 FIRMADDVVCLAFVSAPQNGGVSIYGNILQTNFLIGYDTQKMAVSFRPADCAAM 425

[Close]

BLAST of MS007888 vs. NCBI nr
Match: XP_022964067.1 (aspartic proteinase CDR1-like [Cucurbita moschata])

HSP 1 Score: 563.5 bits (1451), Expect = 1.5e-156
Identity = 279/416 (67.07%), Postives = 331/416 (79.57%), Query Frame = 0


Query: 2   AVVAVDYGFTVELIHRDSPNSPLFNPSETHYHRLTNALRRSISRHAAAPAADVAEAPMYN 61
           A V  +YGF+VE+IHRDSP SP++NPSETHYHRL N LRRSI  + A    D AEAPM+N
Sbjct: 17  AAVNGEYGFSVEMIHRDSPKSPMYNPSETHYHRLANTLRRSILLNKAVALLDTAEAPMFN 76

Query: 62  NRGEYLMKLSLGTPPFPIMAIADTGSDIVWSQCAPCTRCYTQSAPMFDPSKSATYKQILC 121
           +RGEYL+++SLGTPPFPI+AIADTGSDIVW+QC PC +CY Q+APMFDPSKS+TYK I C
Sbjct: 77  DRGEYLVEVSLGTPPFPILAIADTGSDIVWTQCQPCPKCYEQTAPMFDPSKSSTYKIIPC 136

Query: 122 SSPICPFSGTD-SCPVRSDQCEYSINYGDGSHSRGDIAVDTVTLGSTSGRPVAFPRTVFG 181
           SSP C  +G + SC  RS  C+YSI+YGDGSHS GD AVDTVT+GSTSGRPVAFPRTV G
Sbjct: 137 SSPSCALAGQERSCSDRSG-CQYSISYGDGSHSNGDFAVDTVTMGSTSGRPVAFPRTVVG 196

Query: 182 CGRDSAGTFNANVSGIVGLGHGAVSLVSQMGAATAGKFSYCLAPIGSDSIRSSKLSFGAN 241
           CG DSAGTF+ NVSGIVGLG G  SLV QMGAA+ GKFSYCL PIG DS  SSKL+FG+N
Sbjct: 197 CGHDSAGTFSTNVSGIVGLGRGPASLVPQMGAASGGKFSYCLTPIG-DSAESSKLNFGSN 256

Query: 242 AVVAGAGTVSTPIYTDD--GPFYRLKLEAMSVGNNKIKFSTASSSPGDANIIIDSGTTVT 301
           A VAG+GTVSTPI T D    FY L +EAMSVG  + +F  AS+    AN+IIDSGTT+T
Sbjct: 257 AQVAGSGTVSTPIKTSDRFNSFYSLNIEAMSVGGKRFEFPAASALGDGANVIIDSGTTLT 316

Query: 302 LLPPDFYADFGTAVSDGINLERVRDPNQFLDFCFQSTDDDFDAPSITVHFEGGDVKLSRE 361
           +LP +FY+ F TA+SD I+LER  DPNQFLDFCF++T+ DF+ PS+TVHFEG DV L RE
Sbjct: 317 ILPTEFYSTFATAISDSISLERTEDPNQFLDFCFKTTNLDFEVPSVTVHFEGADVPLRRE 376

Query: 362 NVFIRMADDVVCLAFVSAPQNGGVSIYGNILQTNFLIGYDTQKMAVSFRPADCAAM 415
           NVF+ +A++VVCLAF        +SIYGNI Q NFL+GYD  + +VSF+PADC+AM
Sbjct: 377 NVFVMVAENVVCLAF-RGGDGQSISIYGNIAQNNFLVGYDVTRNSVSFKPADCSAM 429

[Close]

BLAST of MS007888 vs. NCBI nr
Match: XP_023514471.1 (aspartic proteinase CDR1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 559.7 bits (1441), Expect = 2.1e-155
Identity = 275/416 (66.11%), Postives = 330/416 (79.33%), Query Frame = 0


Query: 2   AVVAVDYGFTVELIHRDSPNSPLFNPSETHYHRLTNALRRSISRHAAAPAADVAEAPMYN 61
           A  + +YGF+VE+IHRDSP SP++NPSETHYHRL N LRRSI  + A    D AEAPM+N
Sbjct: 17  AAASGEYGFSVEMIHRDSPKSPMYNPSETHYHRLANTLRRSILLNKAVALLDTAEAPMFN 76

Query: 62  NRGEYLMKLSLGTPPFPIMAIADTGSDIVWSQCAPCTRCYTQSAPMFDPSKSATYKQILC 121
           +RGEYL+++SLGTPPFPI+AIADTGSDIVW+QC PC +CY Q+APMFDPSKS+TYK I C
Sbjct: 77  DRGEYLVEVSLGTPPFPILAIADTGSDIVWTQCQPCPKCYEQTAPMFDPSKSSTYKIIPC 136

Query: 122 SSPICPFSGTD-SCPVRSDQCEYSINYGDGSHSRGDIAVDTVTLGSTSGRPVAFPRTVFG 181
           SSP C  +G + SC  RS +C+YS++YGDGSHS GD AVDTVT+GSTSGRPVAFPRTV G
Sbjct: 137 SSPSCTLAGQERSCSDRS-ECQYSVSYGDGSHSNGDFAVDTVTMGSTSGRPVAFPRTVVG 196

Query: 182 CGRDSAGTFNANVSGIVGLGHGAVSLVSQMGAATAGKFSYCLAPIGSDSIRSSKLSFGAN 241
           CG DS GTF+ NVSGIVGLG G  SLV QMGAA+ GKFSYCL PIG DS  SSKL+FG+N
Sbjct: 197 CGHDSGGTFSTNVSGIVGLGRGPASLVPQMGAASGGKFSYCLTPIG-DSAESSKLNFGSN 256

Query: 242 AVVAGAGTVSTPIYTDD--GPFYRLKLEAMSVGNNKIKFSTASSSPGDANIIIDSGTTVT 301
           A VAG+GTVSTPI T D    FY L +EAMSVG  + +F  AS+    AN+IIDSGTT+T
Sbjct: 257 AQVAGSGTVSTPIKTSDRFNSFYSLNIEAMSVGGKRFEFPAASALGDGANVIIDSGTTLT 316

Query: 302 LLPPDFYADFGTAVSDGINLERVRDPNQFLDFCFQSTDDDFDAPSITVHFEGGDVKLSRE 361
           +LP +FY+ F TA+SD I+LER  DPNQFLDFCF++T+ DF+ PS+TVHFEG DV L RE
Sbjct: 317 ILPTEFYSTFATAISDSISLERTEDPNQFLDFCFKTTNLDFEVPSVTVHFEGADVPLRRE 376

Query: 362 NVFIRMADDVVCLAFVSAPQNGGVSIYGNILQTNFLIGYDTQKMAVSFRPADCAAM 415
           NVF+ +A++VVCLAF        +SIYGNI Q NFL+GYD  + +VSF+ ADC+AM
Sbjct: 377 NVFVMVAENVVCLAF-RGGDGQSISIYGNIAQNNFLVGYDVTRNSVSFKQADCSAM 429

[Close]

BLAST of MS007888 vs. NCBI nr
Match: KAG6593735.1 (Aspartic proteinase CDR1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 557.0 bits (1434), Expect = 1.4e-154
Identity = 273/416 (65.62%), Postives = 331/416 (79.57%), Query Frame = 0


Query: 2   AVVAVDYGFTVELIHRDSPNSPLFNPSETHYHRLTNALRRSISRHAAAPAADVAEAPMYN 61
           A  + +YGF+VE+IHRDSP SP++NPSETHYHRL N LRRSI  + A    D AEAPM+N
Sbjct: 17  AAASGEYGFSVEMIHRDSPKSPMYNPSETHYHRLANTLRRSILLNKAVGLLDTAEAPMFN 76

Query: 62  NRGEYLMKLSLGTPPFPIMAIADTGSDIVWSQCAPCTRCYTQSAPMFDPSKSATYKQILC 121
           +RGEYL+++SLGTPPFPI+AIADTGSDIVW+QC PC +CY Q+APMFDP+KS+TYK I C
Sbjct: 77  DRGEYLVEVSLGTPPFPILAIADTGSDIVWTQCQPCPKCYEQTAPMFDPNKSSTYKIIPC 136

Query: 122 SSPICPFSGTD-SCPVRSDQCEYSINYGDGSHSRGDIAVDTVTLGSTSGRPVAFPRTVFG 181
           SSP C  +G + SC  RS +C+YSI+YGDGSHS GD AVDT+T+GSTSGRPVAFPRTV G
Sbjct: 137 SSPSCALAGQERSCSDRS-ECQYSISYGDGSHSNGDFAVDTLTMGSTSGRPVAFPRTVVG 196

Query: 182 CGRDSAGTFNANVSGIVGLGHGAVSLVSQMGAATAGKFSYCLAPIGSDSIRSSKLSFGAN 241
           CG DSAGTF+ NVSGIVGLG G  SLV QMGAA+ GKFSYCL PIG DS +SSKL+FG+N
Sbjct: 197 CGHDSAGTFSTNVSGIVGLGRGPASLVPQMGAASGGKFSYCLTPIG-DSAKSSKLNFGSN 256

Query: 242 AVVAGAGTVSTPIYTDD--GPFYRLKLEAMSVGNNKIKFSTASSSPGDANIIIDSGTTVT 301
           A V G+GTVSTPI T D    FY L +EAMSVG  + +F  AS+    AN+IIDSGTT+T
Sbjct: 257 AQVVGSGTVSTPIKTSDRFNSFYSLNIEAMSVGGKRFEFPAASALGDGANVIIDSGTTLT 316

Query: 302 LLPPDFYADFGTAVSDGINLERVRDPNQFLDFCFQSTDDDFDAPSITVHFEGGDVKLSRE 361
           +LP +FY+ F TA+SD I+LER  DPNQFLDFCF++T+ DF+ PS+TVHFEG DV L RE
Sbjct: 317 ILPTEFYSTFVTAISDSISLERTEDPNQFLDFCFKTTNLDFEVPSVTVHFEGADVPLRRE 376

Query: 362 NVFIRMADDVVCLAFVSAPQNGGVSIYGNILQTNFLIGYDTQKMAVSFRPADCAAM 415
           NVF+ +A++V CLAF        +SIYGNI Q NFL+GYD  + +VSF+PADC+A+
Sbjct: 377 NVFVMVAENVACLAF-RGGDGQSISIYGNIAQNNFLVGYDVTRNSVSFKPADCSAV 429

[Close]

BLAST of MS007888 vs. NCBI nr
Match: XP_023000625.1 (aspartic proteinase CDR1 [Cucurbita maxima])

HSP 1 Score: 556.2 bits (1432), Expect = 2.4e-154
Identity = 273/411 (66.42%), Postives = 327/411 (79.56%), Query Frame = 0


Query: 7   DYGFTVELIHRDSPNSPLFNPSETHYHRLTNALRRSISRHAAAPAADVAEAPMYNNRGEY 66
           +YGF+VE+IHRDSP SP++NPSETHYHRL N LRRSI  + A    D AEAPM+N+RGEY
Sbjct: 22  EYGFSVEMIHRDSPKSPMYNPSETHYHRLANTLRRSILLNKAVALLDTAEAPMFNDRGEY 81

Query: 67  LMKLSLGTPPFPIMAIADTGSDIVWSQCAPCTRCYTQSAPMFDPSKSATYKQILCSSPIC 126
           LM++SLGTPPFPI+AIADTGSDIVW+QC PC +CY Q+APMFDPSKS+TYK I CSSP C
Sbjct: 82  LMEVSLGTPPFPILAIADTGSDIVWTQCQPCPKCYEQTAPMFDPSKSSTYKIIPCSSPSC 141

Query: 127 PFSGTD-SCPVRSDQCEYSINYGDGSHSRGDIAVDTVTLGSTSGRPVAFPRTVFGCGRDS 186
             +G + SC  RS  C+YSI+YGDGSHS GD AVDT+T+GSTSGRPVAFPRTV GCG DS
Sbjct: 142 ALAGQERSCSDRS-VCQYSISYGDGSHSNGDFAVDTLTMGSTSGRPVAFPRTVVGCGHDS 201

Query: 187 AGTFNANVSGIVGLGHGAVSLVSQMGAATAGKFSYCLAPIGSDSIRSSKLSFGANAVVAG 246
           AGTF+ NVSGIVGLG G  SLV QMGAA+ GKFSYCL PIG DS  SSKL+FG+NA VAG
Sbjct: 202 AGTFSTNVSGIVGLGRGPASLVPQMGAASDGKFSYCLTPIG-DSAESSKLNFGSNAQVAG 261

Query: 247 AGTVSTPIYTDD--GPFYRLKLEAMSVGNNKIKFSTASSSPGDANIIIDSGTTVTLLPPD 306
           +GTVSTPI   D    FY L +EAMSVG  + +F  AS+    AN+IIDSGTT+T++P +
Sbjct: 262 SGTVSTPIKISDRFNSFYSLNIEAMSVGGKRFQFPAASALGDGANVIIDSGTTLTIVPTE 321

Query: 307 FYADFGTAVSDGINLERVRDPNQFLDFCFQSTDDDFDAPSITVHFEGGDVKLSRENVFIR 366
           FY+ F TA+SD I+L+R  DPNQFLDFCF++T+ DF+ PS+TVHFEG DV L RENVF+ 
Sbjct: 322 FYSTFATAISDSISLDRTEDPNQFLDFCFKTTNFDFEVPSVTVHFEGADVPLRRENVFVM 381

Query: 367 MADDVVCLAFVSAPQNGGVSIYGNILQTNFLIGYDTQKMAVSFRPADCAAM 415
           +A++VVCLAF        +SIYGNI Q NFL+GYD  + +VSF+P DC+AM
Sbjct: 382 VAENVVCLAF-RGGDGQSISIYGNIAQNNFLVGYDVTRNSVSFKPVDCSAM 429

[Close]

BLAST of MS007888 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 5.3e-117
Identity = 208/412 (50.49%), Postives = 279/412 (67.72%), Query Frame = 0


Query: 9   GFTVELIHRDSPNSPLFNPSETHYHRLTNALRRSISRHAAAPAADVAEAP---MYNNRGE 68
           GFT +LIHRDSP SP +NP ET   RL NA+ RS++R       D    P   + +N GE
Sbjct: 30  GFTADLIHRDSPKSPFYNPMETSSQRLRNAIHRSVNRVFHFTEKDNTPQPQIDLTSNSGE 89

Query: 69  YLMKLSLGTPPFPIMAIADTGSDIVWSQCAPCTRCYTQSAPMFDPSKSATYKQILCSSPI 128
           YLM +S+GTPPFPIMAIADTGSD++W+QCAPC  CYTQ  P+FDP  S+TYK + CSS  
Sbjct: 90  YLMNVSIGTPPFPIMAIADTGSDLLWTQCAPCDDCYTQVDPLFDPKTSSTYKDVSCSSSQ 149

Query: 129 C-PFSGTDSCPVRSDQCEYSINYGDGSHSRGDIAVDTVTLGSTSGRPVAFPRTVFGCGRD 188
           C       SC    + C YS++YGD S+++G+IAVDT+TLGS+  RP+     + GCG +
Sbjct: 150 CTALENQASCSTNDNTCSYSLSYGDNSYTKGNIAVDTLTLGSSDTRPMQLKNIIIGCGHN 209

Query: 189 SAGTFNANVSGIVGLGHGAVSLVSQMGAATAGKFSYCLAPIGSDSIRSSKLSFGANAVVA 248
           +AGTFN   SGIVGLG G VSL+ Q+G +  GKFSYCL P+ S   ++SK++FG NA+V+
Sbjct: 210 NAGTFNKKGSGIVGLGGGPVSLIKQLGDSIDGKFSYCLVPLTSKKDQTSKINFGTNAIVS 269

Query: 249 GAGTVSTPIY--TDDGPFYRLKLEAMSVGNNKIKFSTASSSPGDANIIIDSGTTVTLLPP 308
           G+G VSTP+        FY L L+++SVG+ +I++S + S   + NIIIDSGTT+TLLP 
Sbjct: 270 GSGVVSTPLIAKASQETFYYLTLKSISVGSKQIQYSGSDSESSEGNIIIDSGTTLTLLPT 329

Query: 309 DFYADFGTAVSDGINLERVRDPNQFLDFCFQSTDDDFDAPSITVHFEGGDVKLSRENVFI 368
           +FY++   AV+  I+ E+ +DP   L  C+ +T  D   P IT+HF+G DVKL   N F+
Sbjct: 330 EFYSELEDAVASSIDAEKKQDPQSGLSLCYSAT-GDLKVPVITMHFDGADVKLDSSNAFV 389

Query: 369 RMADDVVCLAFVSAPQNGGVSIYGNILQTNFLIGYDTQKMAVSFRPADCAAM 415
           ++++D+VC AF  +P     SIYGN+ Q NFL+GYDT    VSF+P DCA M
Sbjct: 390 QVSEDLVCFAFRGSP---SFSIYGNVAQMNFLVGYDTVSKTVSFKPTDCAKM 437

[Close]

BLAST of MS007888 vs. ExPASy Swiss-Prot
Match: Q3EBM5 (Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g35615 PE=3 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 4.1e-93
Identity = 187/425 (44.00%), Postives = 268/425 (63.06%), Query Frame = 0


Query: 10  FTVELIHRDSPNSPLFNPSETHYHRLTNALRRSISR----HAAAPAADVAEAPMYNNRGE 69
           F+VELIHRDSP SP++NP  T   RL  A  RS+SR    +      D+ ++ +    GE
Sbjct: 26  FSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSRSRRFNHQLSQTDL-QSGLIGADGE 85

Query: 70  YLMKLSLGTPPFPIMAIADTGSDIVWSQCAPCTRCYTQSAPMFDPSKSATYKQILCSSPI 129
           + M +++GTPP  + AIADTGSD+ W QC PC +CY ++ P+FD  KS+TYK   C S  
Sbjct: 86  FFMSITIGTPPIKVFAIADTGSDLTWVQCKPCQQCYKENGPIFDKKKSSTYKSEPCDSRN 145

Query: 130 C-PFSGTD-SCPVRSDQCEYSINYGDGSHSRGDIAVDTVTLGSTSGRPVAFPRTVFGCGR 189
           C   S T+  C   ++ C+Y  +YGD S S+GD+A +TV++ S SG PV+FP TVFGCG 
Sbjct: 146 CQALSSTERGCDESNNICKYRYSYGDQSFSKGDVATETVSIDSASGSPVSFPGTVFGCGY 205

Query: 190 DSAGTFNANVSGIVGLGHGAVSLVSQMGAATAGKFSYCLAPIGSDSIRSSKLSFGANAVV 249
           ++ GTF+   SGI+GLG G +SL+SQ+G++ + KFSYCL+   + +  +S ++ G N++ 
Sbjct: 206 NNGGTFDETGSGIIGLGGGHLSLISQLGSSISKKFSYCLSHKSATTNGTSVINLGTNSIP 265

Query: 250 AG----AGTVSTPIYTDDGP--FYRLKLEAMSVGNNKIKFSTASSSPGD--------ANI 309
           +     +G VSTP+  D  P  +Y L LEA+SVG  KI ++ +S +P D         NI
Sbjct: 266 SSLSKDSGVVSTPL-VDKEPLTYYYLTLEAISVGKKKIPYTGSSYNPNDDGILSETSGNI 325

Query: 310 IIDSGTTVTLLPPDFYADFGTAVSDGI-NLERVRDPNQFLDFCFQSTDDDFDAPSITVHF 369
           IIDSGTT+TLL   F+  F +AV + +   +RV DP   L  CF+S   +   P ITVHF
Sbjct: 326 IIDSGTTLTLLEAGFFDKFSSAVEESVTGAKRVSDPQGLLSHCFKSGSAEIGLPEITVHF 385

Query: 370 EGGDVKLSRENVFIRMADDVVCLAFVSAPQNGGVSIYGNILQTNFLIGYDTQKMAVSFRP 414
            G DV+LS  N F+++++D+VCL+ V   +   V+IYGN  Q +FL+GYD +   VSF+ 
Sbjct: 386 TGADVRLSPINAFVKLSEDMVCLSMVPTTE---VAIYGNFAQMDFLVGYDLETRTVSFQH 445

[Close]

BLAST of MS007888 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 4.9e-70
Identity = 155/415 (37.35%), Postives = 225/415 (54.22%), Query Frame = 0


Query: 9   GFTVELIHRDSPNSPLFNPSETHYHRLTNALRRSISR----HAAAPAADVAEAPMYNNRG 68
           GF + L H DS        + T +  L  A+ R   R     A        E  +Y   G
Sbjct: 40  GFQIMLEHVDS------GKNLTKFQLLERAIERGSRRLQRLEAMLNGPSGVETSVYAGDG 99

Query: 69  EYLMKLSLGTPPFPIMAIADTGSDIVWSQCAPCTRCYTQSAPMFDPSKSATYKQILCSSP 128
           EYLM LS+GTP  P  AI DTGSD++W+QC PCT+C+ QS P+F+P  S+++  + CSS 
Sbjct: 100 EYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQ 159

Query: 129 ICPFSGTDSCPVRSDQCEYSINYGDGSHSRGDIAVDTVTLGSTSGRPVAFPRTVFGCGRD 188
           +C    + +C   ++ C+Y+  YGDGS ++G +  +T+T GS     V+ P   FGCG +
Sbjct: 160 LCQALSSPTC--SNNFCQYTYGYGDGSETQGSMGTETLTFGS-----VSIPNITFGCGEN 219

Query: 189 SAGTFNANVSGIVGLGHGAVSLVSQMGAATAGKFSYCLAPIGSDSIRSSKLSFGANAVVA 248
           + G    N +G+VG+G G +SL SQ+      KFSYC+ PIGS +  +  L   AN+V A
Sbjct: 220 NQGFGQGNGAGLVGMGRGPLSLPSQLDVT---KFSYCMTPIGSSTPSNLLLGSLANSVTA 279

Query: 249 GAGTVSTPIYTDDGPFYRLKLEAMSVGNNKIKFSTA----SSSPGDANIIIDSGTTVTLL 308
           G+   +    +    FY + L  +SVG+ ++    +    +S+ G   IIIDSGTT+T  
Sbjct: 280 GSPNTTLIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYF 339

Query: 309 PPDFYADFGTAVSDGINLERVRDPNQFLDFCFQSTDD--DFDAPSITVHFEGGDVKLSRE 368
             + Y          INL  V   +   D CFQ+  D  +   P+  +HF+GGD++L  E
Sbjct: 340 VNNAYQSVRQEFISQINLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGGDLELPSE 399

Query: 369 NVFIRMADDVVCLAFVSAPQNGGVSIYGNILQTNFLIGYDTQKMAVSFRPADCAA 414
           N FI  ++ ++CLA  S+ Q  G+SI+GNI Q N L+ YDT    VSF  A C A
Sbjct: 400 NYFISPSNGLICLAMGSSSQ--GMSIFGNIQQQNMLVVYDTGNSVVSFASAQCGA 436

[Close]

BLAST of MS007888 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 1.5e-66
Identity = 144/394 (36.55%), Postives = 224/394 (56.85%), Query Frame = 0


Query: 30  THYHRLTNALRRSISR----HAAAPAADVAEAPMYNNRGEYLMKLSLGTPPFPIMAIADT 89
           T Y  +  A++R   R    +A   ++   E P+Y   GEYLM +++GTP     AI DT
Sbjct: 56  TKYELIKRAIKRGERRMRSINAMLQSSSGIETPVYAGDGEYLMNVAIGTPDSSFSAIMDT 115

Query: 90  GSDIVWSQCAPCTRCYTQSAPMFDPSKSATYKQILCSSPICPFSGTDSCPVRSDQCEYSI 149
           GSD++W+QC PCT+C++Q  P+F+P  S+++  + C S  C    +++C   +++C+Y+ 
Sbjct: 116 GSDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQDLPSETC--NNNECQYTY 175

Query: 150 NYGDGSHSRGDIAVDTVTLGSTSGRPVAFPRTVFGCGRDSAGTFNANVSGIVGLGHGAVS 209
            YGDGS ++G +A +T T  ++S      P   FGCG D+ G    N +G++G+G G +S
Sbjct: 176 GYGDGSTTQGYMATETFTFETSS-----VPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLS 235

Query: 210 LVSQMGAATAGKFSYCLAPIGSDSIRSSKLSFGANAVVAGAGTVSTPIYTDDGP-FYRLK 269
           L SQ+G    G+FSYC+   GS S  +  L   A+ V  G+ + +T I++   P +Y + 
Sbjct: 236 LPSQLG---VGQFSYCMTSYGSSSPSTLALGSAASGVPEGSPS-TTLIHSSLNPTYYYIT 295

Query: 270 LEAMSVGNNKIKFSTAS---SSPGDANIIIDSGTTVTLLPPDFYADFGTAVSDGINLERV 329
           L+ ++VG + +   +++      G   +IIDSGTT+T LP D Y     A +D INL  V
Sbjct: 296 LQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQINLPTV 355

Query: 330 RDPNQFLDFCFQSTDD--DFDAPSITVHFEGGDVKLSRENVFIRMADDVVCLAFVSAPQN 389
            + +  L  CFQ   D      P I++ F+GG + L  +N+ I  A+ V+CLA  S+ Q 
Sbjct: 356 DESSSGLSTCFQQPSDGSTVQVPEISMQFDGGVLNLGEQNILISPAEGVICLAMGSSSQL 415

Query: 390 GGVSIYGNILQTNFLIGYDTQKMAVSFRPADCAA 414
            G+SI+GNI Q    + YD Q +AVSF P  C A
Sbjct: 416 -GISIFGNIQQQETQVLYDLQNLAVSFVPTQCGA 437

[Close]

BLAST of MS007888 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 5.8e-55
Identity = 162/426 (38.03%), Postives = 211/426 (49.53%), Query Frame = 0


Query: 11  TVELIHRDSPNSPLFNPSETHYHRLTNALRR--SISRHAA-APAADVAEAP--------- 70
           T+ L H D+ +S    P E    RL    RR  SI+  AA  P  +V  AP         
Sbjct: 73  TLNLDHIDALSSNK-TPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAPRPGGFSSSV 132

Query: 71  ---MYNNRGEYLMKLSLGTPPFPIMAIADTGSDIVWSQCAPCTRCYTQSAPMFDPSKSAT 130
              +    GEY  +L +GTP   +  + DTGSDIVW QCAPC RCY+QS P+FDP KS T
Sbjct: 133 VSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPRKSKT 192

Query: 131 YKQILCSSPICPFSGTDSCPVRSDQCEYSINYGDGSHSRGDIAVDTVTLGSTSGRPVAFP 190
           Y  I CSSP C    +  C  R   C Y ++YGDGS + GD + +T+T      + VA  
Sbjct: 193 YATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNRVKGVA-- 252

Query: 191 RTVFGCGRDSAGTFNANVSGIVGLGHGAVSLVSQMGAATAGKFSYCLAPIGSDSIRSSKL 250
               GCG D+ G F    +G++GLG G +S   Q G     KFSYCL    S S + S +
Sbjct: 253 ---LGCGHDNEGLF-VGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVD-RSASSKPSSV 312

Query: 251 SFGANAVVAGAGTVSTPIYTDD--GPFYRLKLEAMSVGNNKIKFSTAS----SSPGDANI 310
            FG NA V+      TP+ ++     FY + L  +SVG  ++   TAS       G+  +
Sbjct: 313 VFG-NAAVSRIARF-TPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGV 372

Query: 311 IIDSGTTVTLLPPDFYADFGTAVSDGI-NLERVRDPNQFLDFCFQ-STDDDFDAPSITVH 370
           IIDSGT+VT L    Y     A   G   L+R  D + F D CF  S  ++   P++ +H
Sbjct: 373 IIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLF-DTCFDLSNMNEVKVPTVVLH 432

Query: 371 FEGGDVKLSRENVFIRM-ADDVVCLAFVSAPQNGGVSIYGNILQTNFLIGYDTQKMAVSF 413
           F G DV L   N  I +  +   C AF  A   GG+SI GNI Q  F + YD     V F
Sbjct: 433 FRGADVSLPATNYLIPVDTNGKFCFAF--AGTMGGLSIIGNIQQQGFRVVYDLASSRVGF 485

[Close]

BLAST of MS007888 vs. ExPASy TrEMBL
Match: A0A6J1DNR5 (aspartic proteinase CDR1-like OS=Momordica charantia OX=3673 GN=LOC111022790 PE=3 SV=1)

HSP 1 Score: 819.7 bits (2116), Expect = 5.5e-234
Identity = 407/414 (98.31%), Postives = 407/414 (98.31%), Query Frame = 0


Query: 1   SAVVAVDYGFTVELIHRDSPNSPLFNPSETHYHRLTNALRRSISRHAAAPAADVAEAPMY 60
           SAVVAVDYGFTVELIHRDSPNSPLFNPSETHYHRLTNALRRSISRHAAAPAADVAEAPMY
Sbjct: 12  SAVVAVDYGFTVELIHRDSPNSPLFNPSETHYHRLTNALRRSISRHAAAPAADVAEAPMY 71

Query: 61  NNRGEYLMKLSLGTPPFPIMAIADTGSDIVWSQCAPCTRCYTQSAPMFDPSKSATYKQIL 120
           NNRGEYLMKLSLGTPPFPIMAIADTGSDIVWSQCAPCTRCYTQSAPMFDP KSATYKQIL
Sbjct: 72  NNRGEYLMKLSLGTPPFPIMAIADTGSDIVWSQCAPCTRCYTQSAPMFDPRKSATYKQIL 131

Query: 121 CSSPICPFSGTDSCPVRSDQCEYSINYGDGSHSRGDIAVDTVTLGSTSGRPVAFPRTVFG 180
           CSSPICPFSGTDSCPVRSDQCEYSINYGDGSHSRGDIAVDTVTLGSTSGRPVAFP TVFG
Sbjct: 132 CSSPICPFSGTDSCPVRSDQCEYSINYGDGSHSRGDIAVDTVTLGSTSGRPVAFPWTVFG 191

Query: 181 CGRDSAGTFNANVSGIVGLGHGAVSLVSQMGAATAGKFSYCLAPIGSDSIRSSKLSFGAN 240
           CGRDSAGTFNANVSGIVGLGHG VSLVSQMGAATAGKFSYCLA IGSDSIRSSKLSFGAN
Sbjct: 192 CGRDSAGTFNANVSGIVGLGHGVVSLVSQMGAATAGKFSYCLASIGSDSIRSSKLSFGAN 251

Query: 241 AVVAGAGTVSTPIYTDDGPFYRLKLEAMSVGNNKIKFSTASSSPGDANIIIDSGTTVTLL 300
           AVVAGAGTVSTPIYT DGPFYRLKLEAMSVGNNKI FSTASSSPGDANIIIDSGTTVTLL
Sbjct: 252 AVVAGAGTVSTPIYTADGPFYRLKLEAMSVGNNKITFSTASSSPGDANIIIDSGTTVTLL 311

Query: 301 PPDFYADFGTAVSDGINLERVRDPNQFLDFCFQSTDDDFDAPSITVHFEGGDVKLSRENV 360
           PPDFYADFGTAVSDGINLERVRDPNQFLDFCFQST DDFDAPSITVHFEGGDVKLSRENV
Sbjct: 312 PPDFYADFGTAVSDGINLERVRDPNQFLDFCFQSTADDFDAPSITVHFEGGDVKLSRENV 371

Query: 361 FIRMADDVVCLAFVSAPQNGGVSIYGNILQTNFLIGYDTQKMAVSFRPADCAAM 415
           FIRMADDVVCLAFVSAPQNGGVSIYGNILQTNFLIGYDTQKMAVSFRPADCAAM
Sbjct: 372 FIRMADDVVCLAFVSAPQNGGVSIYGNILQTNFLIGYDTQKMAVSFRPADCAAM 425

[Close]

BLAST of MS007888 vs. ExPASy TrEMBL
Match: A0A6J1HGT9 (aspartic proteinase CDR1-like OS=Cucurbita moschata OX=3662 GN=LOC111464204 PE=3 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 7.1e-157
Identity = 279/416 (67.07%), Postives = 331/416 (79.57%), Query Frame = 0


Query: 2   AVVAVDYGFTVELIHRDSPNSPLFNPSETHYHRLTNALRRSISRHAAAPAADVAEAPMYN 61
           A V  +YGF+VE+IHRDSP SP++NPSETHYHRL N LRRSI  + A    D AEAPM+N
Sbjct: 17  AAVNGEYGFSVEMIHRDSPKSPMYNPSETHYHRLANTLRRSILLNKAVALLDTAEAPMFN 76

Query: 62  NRGEYLMKLSLGTPPFPIMAIADTGSDIVWSQCAPCTRCYTQSAPMFDPSKSATYKQILC 121
           +RGEYL+++SLGTPPFPI+AIADTGSDIVW+QC PC +CY Q+APMFDPSKS+TYK I C
Sbjct: 77  DRGEYLVEVSLGTPPFPILAIADTGSDIVWTQCQPCPKCYEQTAPMFDPSKSSTYKIIPC 136

Query: 122 SSPICPFSGTD-SCPVRSDQCEYSINYGDGSHSRGDIAVDTVTLGSTSGRPVAFPRTVFG 181
           SSP C  +G + SC  RS  C+YSI+YGDGSHS GD AVDTVT+GSTSGRPVAFPRTV G
Sbjct: 137 SSPSCALAGQERSCSDRSG-CQYSISYGDGSHSNGDFAVDTVTMGSTSGRPVAFPRTVVG 196

Query: 182 CGRDSAGTFNANVSGIVGLGHGAVSLVSQMGAATAGKFSYCLAPIGSDSIRSSKLSFGAN 241
           CG DSAGTF+ NVSGIVGLG G  SLV QMGAA+ GKFSYCL PIG DS  SSKL+FG+N
Sbjct: 197 CGHDSAGTFSTNVSGIVGLGRGPASLVPQMGAASGGKFSYCLTPIG-DSAESSKLNFGSN 256

Query: 242 AVVAGAGTVSTPIYTDD--GPFYRLKLEAMSVGNNKIKFSTASSSPGDANIIIDSGTTVT 301
           A VAG+GTVSTPI T D    FY L +EAMSVG  + +F  AS+    AN+IIDSGTT+T
Sbjct: 257 AQVAGSGTVSTPIKTSDRFNSFYSLNIEAMSVGGKRFEFPAASALGDGANVIIDSGTTLT 316

Query: 302 LLPPDFYADFGTAVSDGINLERVRDPNQFLDFCFQSTDDDFDAPSITVHFEGGDVKLSRE 361
           +LP +FY+ F TA+SD I+LER  DPNQFLDFCF++T+ DF+ PS+TVHFEG DV L RE
Sbjct: 317 ILPTEFYSTFATAISDSISLERTEDPNQFLDFCFKTTNLDFEVPSVTVHFEGADVPLRRE 376

Query: 362 NVFIRMADDVVCLAFVSAPQNGGVSIYGNILQTNFLIGYDTQKMAVSFRPADCAAM 415
           NVF+ +A++VVCLAF        +SIYGNI Q NFL+GYD  + +VSF+PADC+AM
Sbjct: 377 NVFVMVAENVVCLAF-RGGDGQSISIYGNIAQNNFLVGYDVTRNSVSFKPADCSAM 429

[Close]

BLAST of MS007888 vs. ExPASy TrEMBL
Match: A0A6J1KIW1 (aspartic proteinase CDR1 OS=Cucurbita maxima OX=3661 GN=LOC111494868 PE=3 SV=1)

HSP 1 Score: 556.2 bits (1432), Expect = 1.1e-154
Identity = 273/411 (66.42%), Postives = 327/411 (79.56%), Query Frame = 0


Query: 7   DYGFTVELIHRDSPNSPLFNPSETHYHRLTNALRRSISRHAAAPAADVAEAPMYNNRGEY 66
           +YGF+VE+IHRDSP SP++NPSETHYHRL N LRRSI  + A    D AEAPM+N+RGEY
Sbjct: 22  EYGFSVEMIHRDSPKSPMYNPSETHYHRLANTLRRSILLNKAVALLDTAEAPMFNDRGEY 81

Query: 67  LMKLSLGTPPFPIMAIADTGSDIVWSQCAPCTRCYTQSAPMFDPSKSATYKQILCSSPIC 126
           LM++SLGTPPFPI+AIADTGSDIVW+QC PC +CY Q+APMFDPSKS+TYK I CSSP C
Sbjct: 82  LMEVSLGTPPFPILAIADTGSDIVWTQCQPCPKCYEQTAPMFDPSKSSTYKIIPCSSPSC 141

Query: 127 PFSGTD-SCPVRSDQCEYSINYGDGSHSRGDIAVDTVTLGSTSGRPVAFPRTVFGCGRDS 186
             +G + SC  RS  C+YSI+YGDGSHS GD AVDT+T+GSTSGRPVAFPRTV GCG DS
Sbjct: 142 ALAGQERSCSDRS-VCQYSISYGDGSHSNGDFAVDTLTMGSTSGRPVAFPRTVVGCGHDS 201

Query: 187 AGTFNANVSGIVGLGHGAVSLVSQMGAATAGKFSYCLAPIGSDSIRSSKLSFGANAVVAG 246
           AGTF+ NVSGIVGLG G  SLV QMGAA+ GKFSYCL PIG DS  SSKL+FG+NA VAG
Sbjct: 202 AGTFSTNVSGIVGLGRGPASLVPQMGAASDGKFSYCLTPIG-DSAESSKLNFGSNAQVAG 261

Query: 247 AGTVSTPIYTDD--GPFYRLKLEAMSVGNNKIKFSTASSSPGDANIIIDSGTTVTLLPPD 306
           +GTVSTPI   D    FY L +EAMSVG  + +F  AS+    AN+IIDSGTT+T++P +
Sbjct: 262 SGTVSTPIKISDRFNSFYSLNIEAMSVGGKRFQFPAASALGDGANVIIDSGTTLTIVPTE 321

Query: 307 FYADFGTAVSDGINLERVRDPNQFLDFCFQSTDDDFDAPSITVHFEGGDVKLSRENVFIR 366
           FY+ F TA+SD I+L+R  DPNQFLDFCF++T+ DF+ PS+TVHFEG DV L RENVF+ 
Sbjct: 322 FYSTFATAISDSISLDRTEDPNQFLDFCFKTTNFDFEVPSVTVHFEGADVPLRRENVFVM 381

Query: 367 MADDVVCLAFVSAPQNGGVSIYGNILQTNFLIGYDTQKMAVSFRPADCAAM 415
           +A++VVCLAF        +SIYGNI Q NFL+GYD  + +VSF+P DC+AM
Sbjct: 382 VAENVVCLAF-RGGDGQSISIYGNIAQNNFLVGYDVTRNSVSFKPVDCSAM 429

[Close]

BLAST of MS007888 vs. ExPASy TrEMBL
Match: A0A6J1HM14 (aspartic proteinase CDR1-like OS=Cucurbita moschata OX=3662 GN=LOC111464202 PE=3 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 5.7e-154
Identity = 272/416 (65.38%), Postives = 330/416 (79.33%), Query Frame = 0


Query: 2   AVVAVDYGFTVELIHRDSPNSPLFNPSETHYHRLTNALRRSISRHAAAPAADVAEAPMYN 61
           A  + +YGF+VE+IHRDSP SP++NPSETHYHRL N LRRSI  + A    D AEAPM+N
Sbjct: 17  AAASGEYGFSVEMIHRDSPKSPMYNPSETHYHRLANTLRRSILLNRAVALLDTAEAPMFN 76

Query: 62  NRGEYLMKLSLGTPPFPIMAIADTGSDIVWSQCAPCTRCYTQSAPMFDPSKSATYKQILC 121
           +RGEYL+++SLGTPPFPI+AIADTGSDIVW+QC PC +CY Q+APMFDPSKS+TYK I C
Sbjct: 77  DRGEYLVEVSLGTPPFPILAIADTGSDIVWTQCQPCPKCYEQTAPMFDPSKSSTYKIIPC 136

Query: 122 SSPICPFSGTD-SCPVRSDQCEYSINYGDGSHSRGDIAVDTVTLGSTSGRPVAFPRTVFG 181
           SSP C  +G + SC  RS +C+YSI+YGDGSHS GD AVDTVT+GSTSGRPVAFPRTV G
Sbjct: 137 SSPSCALAGQERSCSDRS-ECQYSISYGDGSHSNGDFAVDTVTMGSTSGRPVAFPRTVVG 196

Query: 182 CGRDSAGTFNANVSGIVGLGHGAVSLVSQMGAATAGKFSYCLAPIGSDSIRSSKLSFGAN 241
           CG DSAGTF+ NVSGIVGLG G  SLV QMGAA+  KFSYCL PIG DS  SSKL+FG+N
Sbjct: 197 CGHDSAGTFSTNVSGIVGLGRGPASLVPQMGAASGNKFSYCLTPIG-DSAESSKLNFGSN 256

Query: 242 AVVAGAGTVSTPIYTDD--GPFYRLKLEAMSVGNNKIKFSTASSSPGDANIIIDSGTTVT 301
           A VAG+GTVSTPI T D    +Y L +EAMSVG  + +F  AS+    +N+IIDSGTT+T
Sbjct: 257 AQVAGSGTVSTPIKTSDRFDSYYSLNIEAMSVGGKRFEFPAASALGDGSNVIIDSGTTLT 316

Query: 302 LLPPDFYADFGTAVSDGINLERVRDPNQFLDFCFQSTDDDFDAPSITVHFEGGDVKLSRE 361
           +LP +FY+ F TA+S+ I+LER  DPNQFLDFCF++T+ DF+ PS+TVHFEG DV L RE
Sbjct: 317 ILPTEFYSTFATAISESISLERTEDPNQFLDFCFKTTNLDFEVPSVTVHFEGADVPLRRE 376

Query: 362 NVFIRMADDVVCLAFVSAPQNGGVSIYGNILQTNFLIGYDTQKMAVSFRPADCAAM 415
           NVF+ +A++VVCLAF        +SIYGNI Q NF++GYD  +  VSF+PA+C+AM
Sbjct: 377 NVFVMVAENVVCLAF-RGGDGQSISIYGNIAQINFIVGYDVTRNFVSFKPANCSAM 429

[Close]

BLAST of MS007888 vs. ExPASy TrEMBL
Match: A0A1S4E2N4 (aspartic proteinase CDR1-like OS=Cucumis melo OX=3656 GN=LOC107991689 PE=3 SV=1)

HSP 1 Score: 552.7 bits (1423), Expect = 1.3e-153
Identity = 269/418 (64.35%), Postives = 331/418 (79.19%), Query Frame = 0


Query: 1   SAVVAVDYGFTVELIHRDSPNSPLFNPSETHYHRLTNALRRSISRHAAAPAADVAEAPMY 60
           SA  A DYGFTVELIHRDS  SP++N SETHY R+ NALRRSI+R+ A   +D AEAP+Y
Sbjct: 17  SATTARDYGFTVELIHRDSTKSPMYNSSETHYDRIANALRRSINRNKAVLTSDTAEAPIY 76

Query: 61  NNRGEYLMKLSLGTPPFPIMAIADTGSDIVWSQCAPCTRCYTQSAPMFDPSKSATYKQIL 120
           NN GEYL+++S+GTPPF I+A+ADTGSD++W+QC PC+ CY QSAPMFDPSKSATYK + 
Sbjct: 77  NNGGEYLVEISIGTPPFSILAVADTGSDVIWTQCEPCSNCYQQSAPMFDPSKSATYKNVP 136

Query: 121 CSSPICPFSGTDSCPVRSDQCEYSINYGDGSHSRGDIAVDTVTLGSTSGRPVAFPRTVFG 180
           CSSP+C +SG  S      +C YSI YGD SHS G++AVDTVT+ STSGRPVAFPRTV G
Sbjct: 137 CSSPVCSYSGDGSSCSDDSECLYSIAYGDKSHSDGNLAVDTVTMQSTSGRPVAFPRTVIG 196

Query: 181 CGRDSAGTFNANVSGIVGLGHGAVSLVSQMGAATAGKFSYCLAPIGSDSIR-SSKLSFGA 240
           CG D+AGTFNANVSGIVGLG G  SLV+Q+G AT GKFSYCL PIG+ S+  S+KL+FG+
Sbjct: 197 CGHDNAGTFNANVSGIVGLGRGPASLVTQLGPATGGKFSYCLMPIGNASMEDSTKLNFGS 256

Query: 241 NAVVAGAGTVSTPIYTDD--GPFYRLKLEAMSVGNNKIKFSTASSS-PGDANIIIDSGTT 300
           NA V+G+G VSTPIYT D    FY LKLEA+SVG+NK  F   SS   G+ANIIIDSGTT
Sbjct: 257 NADVSGSGAVSTPIYTSDQYKTFYSLKLEAVSVGDNKFDFPEVSSKLGGEANIIIDSGTT 316

Query: 301 VTLLPPDFYADFGTAVSDGINLERVRDPNQFLDFCFQSTDDDFDAPSITVHFEGGDVKLS 360
           +T LP D  ++FG+A++D INL R  DP+QFLD+CF +T DD++ PS+T+HFEG DV L 
Sbjct: 317 LTYLPSDLMSNFGSAIADSINLPRAEDPSQFLDYCFSTTTDDYEVPSVTMHFEGADVPLQ 376

Query: 361 RENVFIRMADDVVCLAFVSAPQNGGVSIYGNILQTNFLIGYDTQKMAVSFRPADCAAM 415
           REN+FIR+++D +CLAF  A  +  + IYGNI Q+NFL+GYD + +AVSF+PADC AM
Sbjct: 377 RENMFIRLSEDTICLAF-GAFSDDNIFIYGNIAQSNFLVGYDIKNLAVSFQPADCNAM 433

[Close]

BLAST of MS007888 vs. TAIR 10
Match: AT5G33340.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 422.5 bits (1085), Expect = 3.8e-118
Identity = 208/412 (50.49%), Postives = 279/412 (67.72%), Query Frame = 0


Query: 9   GFTVELIHRDSPNSPLFNPSETHYHRLTNALRRSISRHAAAPAADVAEAP---MYNNRGE 68
           GFT +LIHRDSP SP +NP ET   RL NA+ RS++R       D    P   + +N GE
Sbjct: 30  GFTADLIHRDSPKSPFYNPMETSSQRLRNAIHRSVNRVFHFTEKDNTPQPQIDLTSNSGE 89

Query: 69  YLMKLSLGTPPFPIMAIADTGSDIVWSQCAPCTRCYTQSAPMFDPSKSATYKQILCSSPI 128
           YLM +S+GTPPFPIMAIADTGSD++W+QCAPC  CYTQ  P+FDP  S+TYK + CSS  
Sbjct: 90  YLMNVSIGTPPFPIMAIADTGSDLLWTQCAPCDDCYTQVDPLFDPKTSSTYKDVSCSSSQ 149

Query: 129 C-PFSGTDSCPVRSDQCEYSINYGDGSHSRGDIAVDTVTLGSTSGRPVAFPRTVFGCGRD 188
           C       SC    + C YS++YGD S+++G+IAVDT+TLGS+  RP+     + GCG +
Sbjct: 150 CTALENQASCSTNDNTCSYSLSYGDNSYTKGNIAVDTLTLGSSDTRPMQLKNIIIGCGHN 209

Query: 189 SAGTFNANVSGIVGLGHGAVSLVSQMGAATAGKFSYCLAPIGSDSIRSSKLSFGANAVVA 248
           +AGTFN   SGIVGLG G VSL+ Q+G +  GKFSYCL P+ S   ++SK++FG NA+V+
Sbjct: 210 NAGTFNKKGSGIVGLGGGPVSLIKQLGDSIDGKFSYCLVPLTSKKDQTSKINFGTNAIVS 269

Query: 249 GAGTVSTPIY--TDDGPFYRLKLEAMSVGNNKIKFSTASSSPGDANIIIDSGTTVTLLPP 308
           G+G VSTP+        FY L L+++SVG+ +I++S + S   + NIIIDSGTT+TLLP 
Sbjct: 270 GSGVVSTPLIAKASQETFYYLTLKSISVGSKQIQYSGSDSESSEGNIIIDSGTTLTLLPT 329

Query: 309 DFYADFGTAVSDGINLERVRDPNQFLDFCFQSTDDDFDAPSITVHFEGGDVKLSRENVFI 368
           +FY++   AV+  I+ E+ +DP   L  C+ +T  D   P IT+HF+G DVKL   N F+
Sbjct: 330 EFYSELEDAVASSIDAEKKQDPQSGLSLCYSAT-GDLKVPVITMHFDGADVKLDSSNAFV 389

Query: 369 RMADDVVCLAFVSAPQNGGVSIYGNILQTNFLIGYDTQKMAVSFRPADCAAM 415
           ++++D+VC AF  +P     SIYGN+ Q NFL+GYDT    VSF+P DCA M
Sbjct: 390 QVSEDLVCFAFRGSP---SFSIYGNVAQMNFLVGYDTVSKTVSFKPTDCAKM 437

[Close]

BLAST of MS007888 vs. TAIR 10
Match: AT1G64830.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 404.8 bits (1039), Expect = 8.2e-113
Identity = 199/413 (48.18%), Postives = 281/413 (68.04%), Query Frame = 0


Query: 9   GFTVELIHRDSPNSPLFNPSETHYHRLTNALRRS------ISRHAAAPAADVAEAPMYNN 68
           GFT++LIHRDSP SP +N +ET   R+ NA+RRS       S   A+P  +  ++ + +N
Sbjct: 25  GFTIDLIHRDSPKSPFYNSAETSSQRMRNAIRRSARSTLQFSNDDASP--NSPQSFITSN 84

Query: 69  RGEYLMKLSLGTPPFPIMAIADTGSDIVWSQCAPCTRCYTQSAPMFDPSKSATYKQILCS 128
           RGEYLM +S+GTPP PI+AIADTGSD++W+QC PC  CY Q++P+FDP +S+TY+++ CS
Sbjct: 85  RGEYLMNISIGTPPVPILAIADTGSDLIWTQCNPCEDCYQQTSPLFDPKESSTYRKVSCS 144

Query: 129 SPICPFSGTDSCPVRSDQCEYSINYGDGSHSRGDIAVDTVTLGSTSGRPVAFPRTVFGCG 188
           S  C      SC    + C Y+I YGD S+++GD+AVDTVT+GS+  RPV+    + GCG
Sbjct: 145 SSQCRALEDASCSTDENTCSYTITYGDNSYTKGDVAVDTVTMGSSGRRPVSLRNMIIGCG 204

Query: 189 RDSAGTFNANVSGIVGLGHGAVSLVSQMGAATAGKFSYCLAPIGSDSIRSSKLSFGANAV 248
            ++ GTF+   SGI+GLG G+ SLVSQ+  +  GKFSYCL P  S++  +SK++FG N +
Sbjct: 205 HENTGTFDPAGSGIIGLGGGSTSLVSQLRKSINGKFSYCLVPFTSETGLTSKINFGTNGI 264

Query: 249 VAGAGTVSTP-IYTDDGPFYRLKLEAMSVGNNKIKFSTASSSPGDANIIIDSGTTVTLLP 308
           V+G G VST  +  D   +Y L LEA+SVG+ KI+F++     G+ NI+IDSGTT+TLLP
Sbjct: 265 VSGDGVVSTSMVKKDPATYYFLNLEAISVGSKKIQFTSTIFGTGEGNIVIDSGTTLTLLP 324

Query: 309 PDFYADFGTAVSDGINLERVRDPNQFLDFCFQSTDDDFDAPSITVHFEGGDVKLSRENVF 368
            +FY +  + V+  I  ERV+DP+  L  C++ +   F  P ITVHF+GGDVKL   N F
Sbjct: 325 SNFYYELESVVASTIKAERVQDPDGILSLCYRDS-SSFKVPDITVHFKGGDVKLGNLNTF 384

Query: 369 IRMADDVVCLAFVSAPQNGGVSIYGNILQTNFLIGYDTQKMAVSFRPADCAAM 415
           + +++DV C AF +   N  ++I+GN+ Q NFL+GYDT    VSF+  DC+ M
Sbjct: 385 VAVSEDVSCFAFAA---NEQLTIFGNLAQMNFLVGYDTVSGTVSFKKTDCSQM 431

[Close]

BLAST of MS007888 vs. TAIR 10
Match: AT1G31450.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 356.7 bits (914), Expect = 2.5e-98
Identity = 186/416 (44.71%), Postives = 261/416 (62.74%), Query Frame = 0


Query: 11  TVELIHRDSPNSPLFNPSETHYHRLTNALRRSISRHAAAPAADVAEAPMYNNRGEYLMKL 70
           TVELIHRDSP+SPL+NP  T   RL  A  RSISR          ++ + +N GEY M +
Sbjct: 30  TVELIHRDSPHSPLYNPHHTVSDRLNAAFLRSISRSRRFTTKTDLQSGLISNGGEYFMSI 89

Query: 71  SLGTPPFPIMAIADTGSDIVWSQCAPCTRCYTQSAPMFDPSKSATYKQILCSSPICP--F 130
           S+GTPP  + AIADTGSD+ W QC PC +CY Q++P+FD  KS+TYK   C S  C    
Sbjct: 90  SIGTPPSKVFAIADTGSDLTWVQCKPCQQCYKQNSPLFDKKKSSTYKTESCDSKTCQALS 149

Query: 131 SGTDSCPVRSDQCEYSINYGDGSHSRGDIAVDTVTLGSTSGRPVAFPRTVFGCGRDSAGT 190
              + C    D C+Y  +YGD S ++GD+A +T+++ S+SG  V+FP TVFGCG ++ GT
Sbjct: 150 EHEEGCDESKDICKYRYSYGDNSFTKGDVATETISIDSSSGSSVSFPGTVFGCGYNNGGT 209

Query: 191 FNANVSGIVGLGHGAVSLVSQMGAATAGKFSYCLAPIGSDSIRSSKLSFGANAVVAG--- 250
           F    SGI+GLG G +SLVSQ+G++   KFSYCL+   + +  +S ++ G N++ +    
Sbjct: 210 FEETGSGIIGLGGGPLSLVSQLGSSIGKKFSYCLSHTAATTNGTSVINLGTNSIPSNPSK 269

Query: 251 -AGTVSTP-IYTDDGPFYRLKLEAMSVGNNKIKFS------TASSSPGDANIIIDSGTTV 310
            + T++TP I  D   +Y L LEA++VG  K+ ++         SS    NIIIDSGTT+
Sbjct: 270 DSATLTTPLIQKDPETYYFLTLEAVTVGKTKLPYTGGGYGLNGKSSKRTGNIIIDSGTTL 329

Query: 311 TLLPPDFYADFGTAVSDGI-NLERVRDPNQFLDFCFQSTDDDFDAPSITVHFEGGDVKLS 370
           TLL   FY DFGTAV + +   +RV DP   L  CF+S D +   P+IT+HF   DVKLS
Sbjct: 330 TLLDSGFYDDFGTAVEESVTGAKRVSDPQGLLTHCFKSGDKEIGLPAITMHFTNADVKLS 389

Query: 371 RENVFIRMADDVVCLAFVSAPQNGGVSIYGNILQTNFLIGYDTQKMAVSFRPADCA 413
             N F+++ +D VCL+ +   +   V+IYGN++Q +FL+GYD +   VSF+  DC+
Sbjct: 390 PINAFVKLNEDTVCLSMIPTTE---VAIYGNMVQMDFLVGYDLETKTVSFQRMDCS 442

[Close]

BLAST of MS007888 vs. TAIR 10
Match: AT2G35615.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 343.2 bits (879), Expect = 2.9e-94
Identity = 187/425 (44.00%), Postives = 268/425 (63.06%), Query Frame = 0


Query: 10  FTVELIHRDSPNSPLFNPSETHYHRLTNALRRSISR----HAAAPAADVAEAPMYNNRGE 69
           F+VELIHRDSP SP++NP  T   RL  A  RS+SR    +      D+ ++ +    GE
Sbjct: 26  FSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSRSRRFNHQLSQTDL-QSGLIGADGE 85

Query: 70  YLMKLSLGTPPFPIMAIADTGSDIVWSQCAPCTRCYTQSAPMFDPSKSATYKQILCSSPI 129
           + M +++GTPP  + AIADTGSD+ W QC PC +CY ++ P+FD  KS+TYK   C S  
Sbjct: 86  FFMSITIGTPPIKVFAIADTGSDLTWVQCKPCQQCYKENGPIFDKKKSSTYKSEPCDSRN 145

Query: 130 C-PFSGTD-SCPVRSDQCEYSINYGDGSHSRGDIAVDTVTLGSTSGRPVAFPRTVFGCGR 189
           C   S T+  C   ++ C+Y  +YGD S S+GD+A +TV++ S SG PV+FP TVFGCG 
Sbjct: 146 CQALSSTERGCDESNNICKYRYSYGDQSFSKGDVATETVSIDSASGSPVSFPGTVFGCGY 205

Query: 190 DSAGTFNANVSGIVGLGHGAVSLVSQMGAATAGKFSYCLAPIGSDSIRSSKLSFGANAVV 249
           ++ GTF+   SGI+GLG G +SL+SQ+G++ + KFSYCL+   + +  +S ++ G N++ 
Sbjct: 206 NNGGTFDETGSGIIGLGGGHLSLISQLGSSISKKFSYCLSHKSATTNGTSVINLGTNSIP 265

Query: 250 AG----AGTVSTPIYTDDGP--FYRLKLEAMSVGNNKIKFSTASSSPGD--------ANI 309
           +     +G VSTP+  D  P  +Y L LEA+SVG  KI ++ +S +P D         NI
Sbjct: 266 SSLSKDSGVVSTPL-VDKEPLTYYYLTLEAISVGKKKIPYTGSSYNPNDDGILSETSGNI 325

Query: 310 IIDSGTTVTLLPPDFYADFGTAVSDGI-NLERVRDPNQFLDFCFQSTDDDFDAPSITVHF 369
           IIDSGTT+TLL   F+  F +AV + +   +RV DP   L  CF+S   +   P ITVHF
Sbjct: 326 IIDSGTTLTLLEAGFFDKFSSAVEESVTGAKRVSDPQGLLSHCFKSGSAEIGLPEITVHF 385

Query: 370 EGGDVKLSRENVFIRMADDVVCLAFVSAPQNGGVSIYGNILQTNFLIGYDTQKMAVSFRP 414
            G DV+LS  N F+++++D+VCL+ V   +   V+IYGN  Q +FL+GYD +   VSF+ 
Sbjct: 386 TGADVRLSPINAFVKLSEDMVCLSMVPTTE---VAIYGNFAQMDFLVGYDLETRTVSFQH 445

[Close]

BLAST of MS007888 vs. TAIR 10
Match: AT2G28010.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 266.9 bits (681), Expect = 2.7e-71
Identity = 162/412 (39.32%), Postives = 233/412 (56.55%), Query Frame = 0


Query: 8   YGFTVELIHRDSPNSPLFNPSETHYHRLTNALRRSISRHAAAPAADVAEAPMYNNRGEYL 67
           +GFT++LIHR S  S                 R S ++  ++P A+     +++N   YL
Sbjct: 28  HGFTMDLIHRRSNASS----------------RVSNTQSGSSPYANT----VFDN-SVYL 87

Query: 68  MKLSLGTPPFPIMAIADTGSDIVWSQCAPCTRCYTQSAPMFDPSKSATYKQILCSSPICP 127
           MKL +GTPPF I AI DTGS+I W+QC PC  CY Q+AP+FDPSKS+T+K+  C    CP
Sbjct: 88  MKLQVGTPPFEIQAIIDTGSEITWTQCLPCVHCYEQNAPIFDPSKSSTFKEKRCDGHSCP 147

Query: 128 FSGTDSCPVRSDQCEYSINYGDGSHSRGDIAVDTVTLGSTSGRPVAFPRTVFGCGRDSAG 187
                          Y ++Y D +++ G +A +T+TL STSG P   P T+ GCG +++ 
Sbjct: 148 ---------------YEVDYFDHTYTMGTLATETITLHSTSGEPFVMPETIIGCGHNNS- 207

Query: 188 TFNANVSGIVGLGHGAVSLVSQMGAATAGKFSYCLAPIGSDSIRSSKLSFGANAVVAGAG 247
            F  + SG+VGL  G  SL++QMG    G  SYC +  G     +SK++FGANA+VAG G
Sbjct: 208 WFKPSFSGMVGLNWGPSSLITQMGGEYPGLMSYCFSGQG-----TSKINFGANAIVAGDG 267

Query: 248 TVSTPIY--TDDGPFYRLKLEAMSVGNNKIKFSTASSSPGDANIIIDSGTTVTLLPPDFY 307
            VST ++  T    FY L L+A+SVGN +I+    +    + NI+IDSGTT+T  P  + 
Sbjct: 268 VVSTTMFMTTAKPGFYYLNLDAVSVGNTRIETMGTTFHALEGNIVIDSGTTLTYFPVSYC 327

Query: 308 ADFGTAVSDGINLERVRDPNQFLDFCFQSTDDDFDAPSITVHFEGG-DVKLSRENVFIRM 367
                AV   +   R  DP      C+ S   D   P IT+HF GG D+ L + N+++  
Sbjct: 328 NLVRQAVEHVVTAVRAADPTGNDMLCYNSDTIDI-FPVITMHFSGGVDLVLDKYNMYMES 387

Query: 368 AD-DVVCLAFV-SAPQNGGVSIYGNILQTNFLIGYDTQKMAVSFRPADCAAM 415
            +  V CLA + ++P     +I+GN  Q NFL+GYD+  + VSF P +C+A+
Sbjct: 388 NNGGVFCLAIICNSPTQ--EAIFGNRAQNNFLVGYDSSSLLVSFSPTNCSAL 394

The following BLAST results are available for this feature:

BLAST of MS007888 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022155743.1	1.1e-233	98.31	aspartic proteinase CDR1-like [Momordica charantia]	[more]
XP_022964067.1	1.5e-156	67.07	aspartic proteinase CDR1-like [Cucurbita moschata]	[more]
XP_023514471.1	2.1e-155	66.11	aspartic proteinase CDR1-like [Cucurbita pepo subsp. pepo]	[more]
KAG6593735.1	1.4e-154	65.63	Aspartic proteinase CDR1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023000625.1	2.4e-154	66.42	aspartic proteinase CDR1 [Cucurbita maxima]	[more]

BLAST of MS007888 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6XBF8	5.3e-117	50.49	Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1	[more]
Q3EBM5	4.1e-93	44.00	Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g3561...	[more]
Q766C3	4.9e-70	37.35	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q766C2	1.5e-66	36.55	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q9LNJ3	5.8e-55	38.03	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]

BLAST of MS007888 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DNR5	5.5e-234	98.31	aspartic proteinase CDR1-like OS=Momordica charantia OX=3673 GN=LOC111022790 PE=...	[more]
A0A6J1HGT9	7.1e-157	67.07	aspartic proteinase CDR1-like OS=Cucurbita moschata OX=3662 GN=LOC111464204 PE=3...	[more]
A0A6J1KIW1	1.1e-154	66.42	aspartic proteinase CDR1 OS=Cucurbita maxima OX=3661 GN=LOC111494868 PE=3 SV=1	[more]
A0A6J1HM14	5.7e-154	65.38	aspartic proteinase CDR1-like OS=Cucurbita moschata OX=3662 GN=LOC111464202 PE=3...	[more]
A0A1S4E2N4	1.3e-153	64.35	aspartic proteinase CDR1-like OS=Cucumis melo OX=3656 GN=LOC107991689 PE=3 SV=1	[more]

BLAST of MS007888 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G33340.1	3.8e-118	50.49	Eukaryotic aspartyl protease family protein	[more]
AT1G64830.1	8.2e-113	48.18	Eukaryotic aspartyl protease family protein	[more]
AT1G31450.1	2.5e-98	44.71	Eukaryotic aspartyl protease family protein	[more]
AT2G35615.1	2.9e-94	44.00	Eukaryotic aspartyl protease family protein	[more]
AT2G28010.1	2.7e-71	39.32	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 238..414 e-value: 1.2E-40 score: 141.1
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 42..237 e-value: 1.5E-55 score: 190.2
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 60..411
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 66..238 e-value: 9.1E-55 score: 185.6
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 261..407 e-value: 1.6E-26 score: 93.0
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 8..412
None	No IPR available	PANTHER	PTHR47967:SF66	ASPARTIC PROTEINASE CDR1-RELATED	coord: 8..412
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 289..300
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 66..407 score: 44.493313
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 65..411 e-value: 4.45613E-87 score: 263.74

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS007888.1	MS007888.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity