MS007814 (gene) Bitter gourd (TR) v1

Overview
NameMS007814
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionPLASMODESMATA CALLOSE-BINDING PROTEIN 3
Locationscaffold13: 1493137 .. 1496119 (+)
RNA-Seq ExpressionMS007814
SyntenyMS007814
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGTGTTGTGTTGCGTTCTCCATGGCAATGCCCACACTTCTCTCTAGCTTCCTCCTTCTCGTGATCGGAAGCTCCGCCGTGGCGGACGGCGCGTACTGGTGCGTGGTCCGGAGCGACGCGAGCGACTCGGCGCTGCAGTCGGCGCTGGACTACGCCTGCGCCACCGGCGCCGAGTGCGCCCCGATTCTGTCGAACGGCCTCTGCTTCCTCCCCAACACCATCCAGGCCCACGCCTCCTACGCCTTCAACAGCTTCTTTCAGCGCAAGGCCATGGCCGCCGGCTCCTGCGACTTCGCCGGCGCCGCCACCATTGCCCAGACCGACCCANNNNNNNNNNNNNNNNNNNCTTTTGAGTTTTATTCATTTTTTTTTCCTCTTCTTTTTCAGGTTATGGATCTTGCGTCTACCCATCTTCCATCAGGTACGCTAATTTCTGATTTTTTTTTCCTTTTTCTGCGATAAAATTTATTTTAGTTGAATTACTGAGTTTGGTTCGGTGGTAGACTAAAAATATATTCTATATGGAATCGAAAATTCACTAGTCAAATTAAACATAGCTTAACTGTTTCTGATAAAAGATTGTAGGTTCGGATATCTACTAATGTTTAATTGGTAAGATATATATTTCTGATAAAAATTTTATCGGTGAAGTTTTATATAGAAAAAATTTTAAAGTGGAAGAATTTATTTGATACAAATTATGAAAGTTTAAGGGATGGAATTTATTTGATACAAATTATGAAAGTTTAAGAGATGGAATTTATTATTTAAATTAGAATGTGCATAGAAAACACTTTATTTTTTTTTTATGGAGATTTCTTCATGTTCACGTAAATGGGTTCTCTTTTTAGGGCAAGATTTGAAAGAATTTGTTTCTCTAAGTCTTATTTGATTTCTTAATCTAGTTTAAGGATTTTAGTTTCTTTTATTATTATTATTATTTTATTATTATTTTTTGTGGGTGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGTAAGAGAGATGGAAAAAGAACCTTTTTTAGGATTTTATTTGAGGATTCATGGGATGGAGGAGTGAATTCCCTAAACATTTATATTCTAAGTTTAATATTTATAATGCATTTACTAATATCTTATTACTTTTTTTTTCACGTAGAATAACTAAATTATATATAACATATAGTTTTTCTCATTTGACATTCAAGAGATATTTCCTTAAATAGTTTTCAAATTGGGGTGGTATGGAAATAAGAATCACGTCTTCTTTTCTCCTTTTGGAAAATCTTTGGTTGAGTATCGATGGTAAAGTATAATTTTTGCCAACATTGTTTTCAAAATTCTCATTTTCTTCCGCTTGATAAAATCAAGATTTTCCTGTCTTTTCTTCTCACATAGAAAATTAATACTCTCTATCTTCAATGCAATTCTTATTACTTAAAAATATATATTTTTTAATCTATCATTTTATTTACTTGTATTTTTATGACATAGAAATGAAAAATAATAAACTTATACATATATTTGAAAAAAATAATGACATTATCAATTACCAAATAATTTAAATTTAATTAAAATATTACTATTTTTTTTAATACAACATGTTAGGGATGAGAGATCGAACTTGCAGTCTCTCGATTTGAGATATATCTCAATTATCACTTAACTACTACTATTTATTTGAAAAAAAAAAGCGGTATAATAATAATACAGATAGAACAGTGTCCAATATCTTTCGGTGGGAATTATCTTAGTTACACACCGTTGGATCAGCGTGGGCCCAGGTTGAGGGCACATCCAACGGTCAGGATAGCATGAATTTATTTTTACAATTTCAAATTAGCTTTTTATTTACTTTTTCTTTATAGAATATTTTGAATTAGCTTTTGAGTGCGCAGGCTGGTCAATAATAATAGTACATGTCAGACTCTGAAAGGTCATTCATTACTGACACTTGTCAGATAAAGCTTGTCCTTTCTTGGGAAAACAAAAAAATAAATTAAAAAATAAACTGAGGAGAAAAAAAAAAGGTAGAAATCACATATTTATCCTGTGAATCTTTGTGTTAAAATTTTACTGTCGAACACAAAATTAAACGTTTAGAGAATTAATAGACACAATTTTAAAAATTTCAATACAAAATTTAGAGAACACATAAGTAATTTAATCAAAATAAATATATCAATTAGTTCTTAAGTTTTAGTTTAATTAACTTCTCTCTTTTTATTTTTTTATCTTTAGTATTACTTCTCCTTTTCCTCTCTTTCTCTCTTCCTTTGCTAGATCTCGGCGATATCGATCCGCTGCAAAATATTTCTGAAGGGATCTCTAAGATGGTCTAGATTTTTTTTTTTTTTTTTTTAAGAAAAAAGACAAGAATAAGAATTAGAAGGAGTGACATGTTGGGTTGTCCCTTAAGGAATCAATTCTTTGTCTAAGAGAATTTGTCCAATTATTTTGCTTATGTTCTCCTTCATTCCTTTGGTCCCTATGGTCCTATAAGTGTCAATAATTTTGCCACCAAGCTTTGTTCAACAATACTTCTTGCCTTGCCCAATGCACTTATCACTGGGCCCACATTTTGAAATTACGTAGCAAACAAAAAATGTTAGGAACCTTACTTTTCTTCAAACCCGTCCTATTTAAGGAAAAAGGTAGATGTACCAAGTTATTCTTACTAAACTTAAAAGATCCATGAAGAAAGATATCTAACAAAGTTCGTTTGTCACAACTTCAGTACTGCAGGAGGACCAATCACACCGACTCCTCCAGCGAACGCAAGCCCAACAATAACAGTGCCAGCCACGACCACCTCACCGAACTTCAACAACGGTGGTACAGATGACACTAATGGACTAAATCCAGTATTCAACCCGACATCAACCACAACTCCAACCAACTCGAAAGCCTCTTCAAGTTTCACGATTCCTAAACTATTGATCTTTGTCTTGTTTTCAATTGCTATCTCACTCACTTTACAAACAATT

mRNA sequence

TTGTGTTGTGTTGCGTTCTCCATGGCAATGCCCACACTTCTCTCTAGCTTCCTCCTTCTCGTGATCGGAAGCTCCGCCGTGGCGGACGGCGCGTACTGGTGCGTGGTCCGGAGCGACGCGAGCGACTCGGCGCTGCAGTCGGCGCTGGACTACGCCTGCGCCACCGGCGCCGAGTGCGCCCCGATTCTGTCGAACGGCCTCTGCTTCCTCCCCAACACCATCCAGGCCCACGCCTCCTACGCCTTCAACAGCTTCTTTCAGCGCAAGGCCATGGCCGCCGGCTCCTGCGACTTCGCCGGCGCCGCCACCATTGCCCAGACCGACCCANNNNNNNNNNNNNNNNNNNCTTTTGATACTGCAGGAGGACCAATCACACCGACTCCTCCAGCGAACGCAAGCCCAACAATAACAGTGCCAGCCACGACCACCTCACCGAACTTCAACAACGGTGGTACAGATGACACTAATGGACTAAATCCAGTATTCAACCCGACATCAACCACAACTCCAACCAACTCGAAAGCCTCTTCAAGTTTCACGATTCCTAAACTATTGATCTTTGTCTTGTTTTCAATTGCTATCTCACTCACTTTACAAACAATT

Coding sequence (CDS)

TTGTGTTGTGTTGCGTTCTCCATGGCAATGCCCACACTTCTCTCTAGCTTCCTCCTTCTCGTGATCGGAAGCTCCGCCGTGGCGGACGGCGCGTACTGGTGCGTGGTCCGGAGCGACGCGAGCGACTCGGCGCTGCAGTCGGCGCTGGACTACGCCTGCGCCACCGGCGCCGAGTGCGCCCCGATTCTGTCGAACGGCCTCTGCTTCCTCCCCAACACCATCCAGGCCCACGCCTCCTACGCCTTCAACAGCTTCTTTCAGCGCAAGGCCATGGCCGCCGGCTCCTGCGACTTCGCCGGCGCCGCCACCATTGCCCAGACCGACCCANNNNNNNNNNNNNNNNNNNCTTTTGATACTGCAGGAGGACCAATCACACCGACTCCTCCAGCGAACGCAAGCCCAACAATAACAGTGCCAGCCACGACCACCTCACCGAACTTCAACAACGGTGGTACAGATGACACTAATGGACTAAATCCAGTATTCAACCCGACATCAACCACAACTCCAACCAACTCGAAAGCCTCTTCAAGTTTCACGATTCCTAAACTATTGATCTTTGTCTTGTTTTCAATTGCTATCTCACTCACTTTACAAACAATT

Protein sequence

LCCVAFSMAMPTLLSSFLLLVIGSSAVADGAYWCVVRSDASDSALQSALDYACATGAECAPILSNGLCFLPNTIQAHASYAFNSFFQRKAMAAGSCDFAGAATIAQTDPXXXXXXXFDTAGGPITPTPPANASPTITVPATTTSPNFNNGGTDDTNGLNPVFNPTSTTTPTNSKASSSFTIPKLLIFVLFSIAISLTLQTI
Homology
BLAST of MS007814 vs. NCBI nr
Match: XP_038875835.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3 [Benincasa hispida])

HSP 1 Score: 255.4 bits (651), Expect = 4.2e-64
Identity = 148/201 (73.63%), Postives = 163/201 (81.09%), Query Frame = 0

Query: 3   CVAFSMAMPTLL--SSFLL--LVIGSSAVADGAYWCVVRSDASDSALQSALDYACATGAE 62
           C  F   M TL+   SFLL   +IG+SAV DGAYWCV RSDAS  ALQ+ALDYACATGA+
Sbjct: 16  CFFFLSQMATLVFSHSFLLFFFLIGTSAV-DGAYWCVARSDASYEALQAALDYACATGAD 75

Query: 63  CAPILSNGLCFLPNTIQAHASYAFNSFFQRKAMAAGSCDFAGAATIAQTDP---XXXXXX 122
           C PIL NGLCFLPNTIQAHASYAFNSFFQRKAMA GSCDFAG+ATIAQ+DP         
Sbjct: 76  CNPILLNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFAGSATIAQSDPSYGSCVYPS 135

Query: 123 XFDTAGGPITPTPPANASPTITVPATTTSPNFNNGGTDDTNGLNPVFNPTSTTTPTNSKA 182
               AGG ITP+PP N SPTITVPATTT P+FNNGGT DTNGLNPV+NPTST++PTNS+A
Sbjct: 136 STSAAGGAITPSPPTNTSPTITVPATTT-PSFNNGGTSDTNGLNPVYNPTSTSSPTNSRA 195

Query: 183 SSSFTIPKLLIFVLFSIAISL 197
           SSSFT+PKLL+ VLFSIAISL
Sbjct: 196 SSSFTVPKLLVLVLFSIAISL 214

BLAST of MS007814 vs. NCBI nr
Match: XP_008457240.1 (PREDICTED: PLASMODESMATA CALLOSE-BINDING PROTEIN 3 [Cucumis melo])

HSP 1 Score: 251.1 bits (640), Expect = 7.9e-63
Identity = 137/188 (72.87%), Postives = 156/188 (82.98%), Query Frame = 0

Query: 17  FLLLVIGSSAVADGAYWCVVRSDASDSALQSALDYACATGAECAPILSNGLCFLPNTIQA 76
           FL  +IG+SAV DGAYWCV RSDA+  +LQ+A+DYACATGA+C PIL NGLCFLPNTIQA
Sbjct: 17  FLFFLIGTSAV-DGAYWCVARSDATYESLQAAIDYACATGADCTPILLNGLCFLPNTIQA 76

Query: 77  HASYAFNSFFQRKAMAAGSCDFAGAATIAQTDP---XXXXXXXFDTAGGPITPTPPANAS 136
           HASYAFNSFFQRKAMA GSCDF G+ATIAQ+DP            +AGG ITP+PPAN S
Sbjct: 77  HASYAFNSFFQRKAMAPGSCDFGGSATIAQSDPSYGSCVYPSSLSSAGGAITPSPPANTS 136

Query: 137 PTITVPATTTSPNFNNGGTDDTNGLNPVFNPTSTTTPTNSKASSSFTIPKLLIFVLFSIA 196
           PTITVP T T P+FNNGGT DTNG+NPV+NPTST+ PTNS+ASSSFT PKL+I +LFSIA
Sbjct: 137 PTITVPGTAT-PSFNNGGTGDTNGVNPVYNPTSTSNPTNSEASSSFTTPKLVILILFSIA 196

Query: 197 ISLTLQTI 202
           ISL LQT+
Sbjct: 197 ISLPLQTM 202

BLAST of MS007814 vs. NCBI nr
Match: KAE8651770.1 (hypothetical protein Csa_006265 [Cucumis sativus])

HSP 1 Score: 246.1 bits (627), Expect = 2.5e-61
Identity = 136/188 (72.34%), Postives = 152/188 (80.85%), Query Frame = 0

Query: 17  FLLLVIGSSAVADGAYWCVVRSDASDSALQSALDYACATGAECAPILSNGLCFLPNTIQA 76
           F   + G+S V DGAYWCV RSDA+  +LQ+ALDYACATGAEC PI+ NGLCFLPNTIQA
Sbjct: 43  FFFFLFGTSTV-DGAYWCVARSDATYESLQAALDYACATGAECTPIMLNGLCFLPNTIQA 102

Query: 77  HASYAFNSFFQRKAMAAGSCDFAGAATIAQTDP---XXXXXXXFDTAGGPITPTPPANAS 136
           HASYAFNSFFQRKAMA GSCDFAG+ATIAQ+DP            +AGG ITP+PPANAS
Sbjct: 103 HASYAFNSFFQRKAMAPGSCDFAGSATIAQSDPSYGSCVYPSSLSSAGGAITPSPPANAS 162

Query: 137 PTITVPATTTSPNFNNGGTDDTNGLNPVFNPTSTTTPTNSKASSSFTIPKLLIFVLFSIA 196
           PTITVP T T PNFNNGGT DTNGLNP +NPTST+ PTNS+AS S T PKL+I +LFSIA
Sbjct: 163 PTITVPGTAT-PNFNNGGTSDTNGLNPDYNPTSTSNPTNSEASLSSTTPKLVILILFSIA 222

Query: 197 ISLTLQTI 202
           ISL LQT+
Sbjct: 223 ISLPLQTM 228

BLAST of MS007814 vs. NCBI nr
Match: XP_004138961.2 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3 [Cucumis sativus])

HSP 1 Score: 246.1 bits (627), Expect = 2.5e-61
Identity = 136/188 (72.34%), Postives = 152/188 (80.85%), Query Frame = 0

Query: 17  FLLLVIGSSAVADGAYWCVVRSDASDSALQSALDYACATGAECAPILSNGLCFLPNTIQA 76
           F   + G+S V DGAYWCV RSDA+  +LQ+ALDYACATGAEC PI+ NGLCFLPNTIQA
Sbjct: 15  FFFFLFGTSTV-DGAYWCVARSDATYESLQAALDYACATGAECTPIMLNGLCFLPNTIQA 74

Query: 77  HASYAFNSFFQRKAMAAGSCDFAGAATIAQTDP---XXXXXXXFDTAGGPITPTPPANAS 136
           HASYAFNSFFQRKAMA GSCDFAG+ATIAQ+DP            +AGG ITP+PPANAS
Sbjct: 75  HASYAFNSFFQRKAMAPGSCDFAGSATIAQSDPSYGSCVYPSSLSSAGGAITPSPPANAS 134

Query: 137 PTITVPATTTSPNFNNGGTDDTNGLNPVFNPTSTTTPTNSKASSSFTIPKLLIFVLFSIA 196
           PTITVP T T PNFNNGGT DTNGLNP +NPTST+ PTNS+AS S T PKL+I +LFSIA
Sbjct: 135 PTITVPGTAT-PNFNNGGTSDTNGLNPDYNPTSTSNPTNSEASLSSTTPKLVILILFSIA 194

Query: 197 ISLTLQTI 202
           ISL LQT+
Sbjct: 195 ISLPLQTM 200

BLAST of MS007814 vs. NCBI nr
Match: XP_022959424.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like [Cucurbita moschata] >XP_023548894.1 PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 240.4 bits (612), Expect = 1.4e-59
Identity = 143/203 (70.44%), Postives = 158/203 (77.83%), Query Frame = 0

Query: 8   MAMPTLLSSFLLL----VIGSSAVADGAYWCVVRSDASDSALQSALDYACATGAECAPIL 67
           MA   L  SFLLL    ++ +SAV DGAYWCV RS+AS  ALQ+ALDYACATGA+C P+L
Sbjct: 1   MATLVLSHSFLLLLLLFLLPTSAV-DGAYWCVARSEASYEALQTALDYACATGADCTPLL 60

Query: 68  SNGLCFLPNTIQAHASYAFNSFFQRKAMAAGSCDFAGAATIAQTDP---XXXXXXXFDTA 127
           SNGLCFLPNTIQAHASYAFNSFFQRKAMA GSCDFAG+ATIAQTDP             A
Sbjct: 61  SNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFAGSATIAQTDPSYGSCVYPSSISAA 120

Query: 128 GGPITPTPPANAS--PTITVPATTTSPNFNNGGTDDTNGLNPVFNPTSTTTPTNSKASSS 187
           GG ITP+PPAN S  PTITVPATTT+P+FNNG T D NGL+PV+NP ST+TPTNSKASSS
Sbjct: 121 GGAITPSPPANTSPTPTITVPATTTTPSFNNGNTGDMNGLDPVYNPASTSTPTNSKASSS 180

Query: 188 FTIPKLLIFVLFSIAISLTLQTI 202
           F IPKL    LFSIAISL   T+
Sbjct: 181 FRIPKL----LFSIAISLASLTL 198

BLAST of MS007814 vs. ExPASy Swiss-Prot
Match: Q9FZ86 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3 OS=Arabidopsis thaliana OX=3702 GN=PDCB3 PE=1 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 4.5e-21
Identity = 60/154 (38.96%), Postives = 85/154 (55.19%), Query Frame = 0

Query: 18  LLLVIGSSAVADGAYWCVVRSDASDSALQSALDYACATGAECAPILSNGLCFLPNTIQAH 77
           L++++ + A      WCV +   S++ LQ  LDYAC  GA+C PI   G CF PNT+++H
Sbjct: 6   LVMILLAMAGHSSGTWCVCKEGLSEAMLQKTLDYACGAGADCGPIHQTGPCFNPNTVKSH 65

Query: 78  ASYAFNSFFQRKAMAAGSCDFAGAATIAQTDPXXXXXXXFDTAGGPITPTPPANASPTIT 137
            SYA NSFFQ+K  + G+CDFAG AT + +DP         +A G  T T P   +P+  
Sbjct: 66  CSYAVNSFFQKKGQSLGTCDFAGTATFSASDPSYTTCPFPASASGSGT-TTPVTTTPSTR 125

Query: 138 VPATTTSPNF-----NNGGTDDTNGLNPVFNPTS 167
           VP TT +  +       GG    +G+NP +   S
Sbjct: 126 VPTTTNTRPYTITPSTGGGLGIPSGINPDYTDPS 158

BLAST of MS007814 vs. ExPASy Swiss-Prot
Match: Q9SD84 (PLASMODESMATA CALLOSE-BINDING PROTEIN 2 OS=Arabidopsis thaliana OX=3702 GN=PDCB2 PE=1 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 8.0e-18
Identity = 60/157 (38.22%), Postives = 81/157 (51.59%), Query Frame = 0

Query: 11  PTLLSSFLLLVIGSSAVADGAYWCVVRSDASDSALQSALDYACATGAECAPILSNGLCFL 70
           P +L    LL+ G ++    A WCV ++  SDS LQ  LDYAC  GA+C P    G CF 
Sbjct: 3   PLVLYLLTLLMAGHTS----ASWCVCKTGLSDSVLQKTLDYACGNGADCNPTHPKGSCFN 62

Query: 71  PNTIQAHASYAFNSFFQRKAMAAGSCDFAGAATIAQTDPXXXXXXXFDTAGGPITPTPPA 130
           P+ ++AH +YA NSFFQ+K  A+ SC+F G AT+  TDP         +A G       +
Sbjct: 63  PDNVRAHCNYAVNSFFQKKGQASESCNFTGTATLTTTDPSYTGCAFPSSASG-------S 122

Query: 131 NASPTITVPATTTSPNFNNGGTDDTNGLNPVFNPTST 168
           + S + TV     SP  +N  T    G +P     ST
Sbjct: 123 SGSGSTTVTPGKNSPKGSNSITTFPGGNSPYSGTPST 148

BLAST of MS007814 vs. ExPASy Swiss-Prot
Match: Q84V39 (Major pollen allergen Ole e 10 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 2.0e-16
Identity = 39/78 (50.00%), Postives = 55/78 (70.51%), Query Frame = 0

Query: 33  WCVVRSDASDSALQSALDYACA-TGAECAPILSNGLCFLPNTIQAHASYAFNSFFQRKAM 92
           WCV +++A+D+ LQS +DY C+ +G +C PI +NG CF PNT++AHASYA NS++Q K  
Sbjct: 37  WCVPKAEATDAQLQSNIDYVCSQSGMDCGPIQANGACFNPNTVRAHASYAMNSWYQSKGR 96

Query: 93  AAGSCDFAGAATIAQTDP 110
               CDF+G   I  +DP
Sbjct: 97  NDFDCDFSGTGAITSSDP 114

BLAST of MS007814 vs. ExPASy Swiss-Prot
Match: Q9FNQ2 (PLASMODESMATA CALLOSE-BINDING PROTEIN 1 OS=Arabidopsis thaliana OX=3702 GN=PDCB1 PE=1 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 2.0e-16
Identity = 69/191 (36.13%), Postives = 97/191 (50.79%), Query Frame = 0

Query: 10  MPTLLSSFLLLVIGSSAVADGAYWCVVRSDASDSALQSALDYACATGAECAPILSNGLCF 69
           M  L+ S LLL   S A    A WCV ++  SD+ LQ+ LDYAC  GA+C P      CF
Sbjct: 1   MAALVLSLLLL---SLAGHSSASWCVCKTGLSDTVLQATLDYACGNGADCNPTKPKQSCF 60

Query: 70  LPNTIQAHASYAFNSFFQRKAMAAGSCDFAGAATIAQTDPXXXXXXXFDTAGG-----PI 129
            P+ +++H +YA NSFFQ+K  + GSC+F G AT   +DP         +A G      +
Sbjct: 61  NPDNVRSHCNYAVNSFFQKKGQSPGSCNFDGTATPTNSDPSYTGCAFPTSASGSSGSTTV 120

Query: 130 TP--TPPANASPTITVPATTTSPNFNNGGTDDTNGLNPVFNPTST-TTPTNSKASSSFTI 189
           TP  T P  +  T T+P + T+  ++   T+   G N     T T   P  +  SS+F +
Sbjct: 121 TPGTTNPKGSPTTTTLPGSGTNSPYSGNPTNGVFGGNSTGGTTGTGINPDYTTDSSAFAL 180

BLAST of MS007814 vs. ExPASy Swiss-Prot
Match: Q93V72 (PLASMODESMATA CALLOSE-BINDING PROTEIN 4 OS=Arabidopsis thaliana OX=3702 GN=PDCB4 PE=1 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 7.0e-14
Identity = 62/176 (35.23%), Postives = 87/176 (49.43%), Query Frame = 0

Query: 18  LLLVIGSSAVADGAYWCVVRSDASDSALQSALDYACATGAECAPILSNGLCFLPNTIQAH 77
           L L+I     ++ AY C+ + + ++  LQ A+DYAC  GA+C  I   G C+ PNT++ H
Sbjct: 7   LCLIISMFTYSNAAY-CLCK-EGNEQVLQKAIDYACGNGADCTQIQPTGACYQPNTVKNH 66

Query: 78  ASYAFNSFFQRKAMAAGSCDFAGAATIAQTDP-------XXXXXXXFDTAGGPITPTP-- 137
              A NS++Q+KA +  +CDF GAA+ + T P                T G P T TP  
Sbjct: 67  CDVAVNSYYQKKASSGATCDFNGAASPSTTPPSTASNCLTGSSSSGTPTTGTPTTGTPTS 126

Query: 138 --PANASPTITVPAT------TTSPNFNNGGTDDTNGLNPVFNPTSTTTPTNSKAS 177
             P   +PT   P T      T +  F N GT +T G N    P S   PT+S +S
Sbjct: 127 GTPTTGTPTTGTPTTGTPTSGTPTSGFPNTGTPNT-GTNTGM-PNSNGMPTSSSSS 178

BLAST of MS007814 vs. ExPASy TrEMBL
Match: A0A1S3C4M3 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3 OS=Cucumis melo OX=3656 GN=LOC103496965 PE=4 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 3.8e-63
Identity = 137/188 (72.87%), Postives = 156/188 (82.98%), Query Frame = 0

Query: 17  FLLLVIGSSAVADGAYWCVVRSDASDSALQSALDYACATGAECAPILSNGLCFLPNTIQA 76
           FL  +IG+SAV DGAYWCV RSDA+  +LQ+A+DYACATGA+C PIL NGLCFLPNTIQA
Sbjct: 17  FLFFLIGTSAV-DGAYWCVARSDATYESLQAAIDYACATGADCTPILLNGLCFLPNTIQA 76

Query: 77  HASYAFNSFFQRKAMAAGSCDFAGAATIAQTDP---XXXXXXXFDTAGGPITPTPPANAS 136
           HASYAFNSFFQRKAMA GSCDF G+ATIAQ+DP            +AGG ITP+PPAN S
Sbjct: 77  HASYAFNSFFQRKAMAPGSCDFGGSATIAQSDPSYGSCVYPSSLSSAGGAITPSPPANTS 136

Query: 137 PTITVPATTTSPNFNNGGTDDTNGLNPVFNPTSTTTPTNSKASSSFTIPKLLIFVLFSIA 196
           PTITVP T T P+FNNGGT DTNG+NPV+NPTST+ PTNS+ASSSFT PKL+I +LFSIA
Sbjct: 137 PTITVPGTAT-PSFNNGGTGDTNGVNPVYNPTSTSNPTNSEASSSFTTPKLVILILFSIA 196

Query: 197 ISLTLQTI 202
           ISL LQT+
Sbjct: 197 ISLPLQTM 202

BLAST of MS007814 vs. ExPASy TrEMBL
Match: A0A0A0LND4 (X8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G139290 PE=4 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 1.2e-61
Identity = 136/188 (72.34%), Postives = 152/188 (80.85%), Query Frame = 0

Query: 17  FLLLVIGSSAVADGAYWCVVRSDASDSALQSALDYACATGAECAPILSNGLCFLPNTIQA 76
           F   + G+S V DGAYWCV RSDA+  +LQ+ALDYACATGAEC PI+ NGLCFLPNTIQA
Sbjct: 15  FFFFLFGTSTV-DGAYWCVARSDATYESLQAALDYACATGAECTPIMLNGLCFLPNTIQA 74

Query: 77  HASYAFNSFFQRKAMAAGSCDFAGAATIAQTDP---XXXXXXXFDTAGGPITPTPPANAS 136
           HASYAFNSFFQRKAMA GSCDFAG+ATIAQ+DP            +AGG ITP+PPANAS
Sbjct: 75  HASYAFNSFFQRKAMAPGSCDFAGSATIAQSDPSYGSCVYPSSLSSAGGAITPSPPANAS 134

Query: 137 PTITVPATTTSPNFNNGGTDDTNGLNPVFNPTSTTTPTNSKASSSFTIPKLLIFVLFSIA 196
           PTITVP T T PNFNNGGT DTNGLNP +NPTST+ PTNS+AS S T PKL+I +LFSIA
Sbjct: 135 PTITVPGTAT-PNFNNGGTSDTNGLNPDYNPTSTSNPTNSEASLSSTTPKLVILILFSIA 194

Query: 197 ISLTLQTI 202
           ISL LQT+
Sbjct: 195 ISLPLQTM 200

BLAST of MS007814 vs. ExPASy TrEMBL
Match: A0A6J1H812 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like OS=Cucurbita moschata OX=3662 GN=LOC111460407 PE=4 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 6.7e-60
Identity = 143/203 (70.44%), Postives = 158/203 (77.83%), Query Frame = 0

Query: 8   MAMPTLLSSFLLL----VIGSSAVADGAYWCVVRSDASDSALQSALDYACATGAECAPIL 67
           MA   L  SFLLL    ++ +SAV DGAYWCV RS+AS  ALQ+ALDYACATGA+C P+L
Sbjct: 1   MATLVLSHSFLLLLLLFLLPTSAV-DGAYWCVARSEASYEALQTALDYACATGADCTPLL 60

Query: 68  SNGLCFLPNTIQAHASYAFNSFFQRKAMAAGSCDFAGAATIAQTDP---XXXXXXXFDTA 127
           SNGLCFLPNTIQAHASYAFNSFFQRKAMA GSCDFAG+ATIAQTDP             A
Sbjct: 61  SNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFAGSATIAQTDPSYGSCVYPSSISAA 120

Query: 128 GGPITPTPPANAS--PTITVPATTTSPNFNNGGTDDTNGLNPVFNPTSTTTPTNSKASSS 187
           GG ITP+PPAN S  PTITVPATTT+P+FNNG T D NGL+PV+NP ST+TPTNSKASSS
Sbjct: 121 GGAITPSPPANTSPTPTITVPATTTTPSFNNGNTGDMNGLDPVYNPASTSTPTNSKASSS 180

Query: 188 FTIPKLLIFVLFSIAISLTLQTI 202
           F IPKL    LFSIAISL   T+
Sbjct: 181 FRIPKL----LFSIAISLASLTL 198

BLAST of MS007814 vs. ExPASy TrEMBL
Match: A0A6J1KVB1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3 OS=Cucurbita maxima OX=3661 GN=LOC111499021 PE=4 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 2.0e-59
Identity = 141/203 (69.46%), Postives = 158/203 (77.83%), Query Frame = 0

Query: 8   MAMPTLLSSFLLL----VIGSSAVADGAYWCVVRSDASDSALQSALDYACATGAECAPIL 67
           MA   L  SFLLL    ++ +SAV DGAYWCV RS+AS  ALQ+ALDYACATGA+C P+L
Sbjct: 1   MATLVLSHSFLLLLLLFLLPTSAV-DGAYWCVARSEASYEALQTALDYACATGADCTPLL 60

Query: 68  SNGLCFLPNTIQAHASYAFNSFFQRKAMAAGSCDFAGAATIAQTDP---XXXXXXXFDTA 127
           SNGLCFLPNTIQAHASYAFNSFFQRKAMA GSCDFAG+AT+AQTDP             A
Sbjct: 61  SNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFAGSATVAQTDPSYGSCVYPSSISAA 120

Query: 128 GGPITPTPPANAS--PTITVPATTTSPNFNNGGTDDTNGLNPVFNPTSTTTPTNSKASSS 187
           GG ITP+PPAN S  PTITVPATTT+P+FNNG + D NGL+PV+NP ST+TPTNSKASSS
Sbjct: 121 GGAITPSPPANTSPTPTITVPATTTTPSFNNGNSGDINGLDPVYNPASTSTPTNSKASSS 180

Query: 188 FTIPKLLIFVLFSIAISLTLQTI 202
           F IPK     LFSIAISLT  T+
Sbjct: 181 FRIPKF----LFSIAISLTSLTL 198

BLAST of MS007814 vs. ExPASy TrEMBL
Match: A0A6J1HKA9 (PLASMODESMATA CALLOSE-BINDING PROTEIN 1-like OS=Cucurbita moschata OX=3662 GN=LOC111464333 PE=4 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 2.2e-55
Identity = 132/197 (67.01%), Postives = 148/197 (75.13%), Query Frame = 0

Query: 8   MAMPTLLSSFLLLVIGSSAVADGA----YWCVVRSDASDSALQSALDYACATGAECAPIL 67
           MA   L  SFLLL+IG++     A    YWCV RSD SD ALQ+AL+YACATGA+C PIL
Sbjct: 1   MATLFLSHSFLLLLIGATTTTTSAISSEYWCVARSDVSDEALQTALNYACATGADCTPIL 60

Query: 68  SNGLCFLPNTIQAHASYAFNSFFQRKAMAAGSCDFAGAATIAQTDP---XXXXXXXFDTA 127
            NGLCFLPNTIQAHASYAFNSFFQRKAMA GSC+FAG+ATIA ++P            TA
Sbjct: 61  PNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCNFAGSATIAHSNPSYGSCVYPSSISTA 120

Query: 128 GGPITPTPPANASPTITVPATT-TSPNFNNGGTDDTNGLNPVFNPTSTTTPTNSKASSSF 187
           GG ITP+ P N SPTIT P TT T+P+FNNGGTDDTNGLNPV+NPTS    TN+KASSS 
Sbjct: 121 GGAITPSLPGNTSPTITTPTTTPTTPSFNNGGTDDTNGLNPVYNPTS----TNAKASSSL 180

Query: 188 TIPKLLIFVLFSIAISL 197
           T  KLLI +L SIAISL
Sbjct: 181 TTSKLLILILLSIAISL 193

BLAST of MS007814 vs. TAIR 10
Match: AT4G13600.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 138.3 bits (347), Expect = 6.9e-33
Identity = 97/203 (47.78%), Postives = 121/203 (59.61%), Query Frame = 0

Query: 6   FSMAMPTLLSSFLLLVIGSSAVADGAYWCVVRSDASDSALQSALDYACATGAECAPILSN 65
           F MA+     SFLLL++  S+    A WCV R D +  ALQ+ALDYACA GA+CAPI  N
Sbjct: 37  FPMALCLQTLSFLLLLLFRSS---AAMWCVARFDVTSQALQAALDYACAAGADCAPIQPN 96

Query: 66  GLCFLPNTIQAHASYAFNSFFQRKAMAAGSCDFAGAATIAQTDPXXXXXXXFDT---AGG 125
           GLCFLPNT+QAHASYAFNS+FQR AMA GSC+FAG +TIA+TDP        ++   AGG
Sbjct: 97  GLCFLPNTVQAHASYAFNSYFQRAAMAPGSCNFAGTSTIAKTDPSYGSCVYPNSVRNAGG 156

Query: 126 PI-------TPTPPANASP--TITVPATTTSPNFNNGGTDDTNGLNPVFNPTSTTTPTNS 185
                    TP+     SP  T+  P+ TT+  F  GG     GL    NP  TTT TN+
Sbjct: 157 SASTTTVGGTPSTTVGNSPMTTLRPPSGTTTSPFGIGG----GGL----NPQGTTTTTNT 216

Query: 186 KASSSFTIPKLLIFVLFSIAISL 197
             S S+ I K  + +   +A+ L
Sbjct: 217 DESGSYIINKSTVSIALFVALLL 228

BLAST of MS007814 vs. TAIR 10
Match: AT1G18650.1 (plasmodesmata callose-binding protein 3 )

HSP 1 Score: 102.8 bits (255), Expect = 3.2e-22
Identity = 60/154 (38.96%), Postives = 85/154 (55.19%), Query Frame = 0

Query: 18  LLLVIGSSAVADGAYWCVVRSDASDSALQSALDYACATGAECAPILSNGLCFLPNTIQAH 77
           L++++ + A      WCV +   S++ LQ  LDYAC  GA+C PI   G CF PNT+++H
Sbjct: 6   LVMILLAMAGHSSGTWCVCKEGLSEAMLQKTLDYACGAGADCGPIHQTGPCFNPNTVKSH 65

Query: 78  ASYAFNSFFQRKAMAAGSCDFAGAATIAQTDPXXXXXXXFDTAGGPITPTPPANASPTIT 137
            SYA NSFFQ+K  + G+CDFAG AT + +DP         +A G  T T P   +P+  
Sbjct: 66  CSYAVNSFFQKKGQSLGTCDFAGTATFSASDPSYTTCPFPASASGSGT-TTPVTTTPSTR 125

Query: 138 VPATTTSPNF-----NNGGTDDTNGLNPVFNPTS 167
           VP TT +  +       GG    +G+NP +   S
Sbjct: 126 VPTTTNTRPYTITPSTGGGLGIPSGINPDYTDPS 158

BLAST of MS007814 vs. TAIR 10
Match: AT5G08000.1 (glucan endo-1,3-beta-glucosidase-like protein 3 )

HSP 1 Score: 92.0 bits (227), Expect = 5.7e-19
Identity = 60/157 (38.22%), Postives = 81/157 (51.59%), Query Frame = 0

Query: 11  PTLLSSFLLLVIGSSAVADGAYWCVVRSDASDSALQSALDYACATGAECAPILSNGLCFL 70
           P +L    LL+ G ++    A WCV ++  SDS LQ  LDYAC  GA+C P    G CF 
Sbjct: 3   PLVLYLLTLLMAGHTS----ASWCVCKTGLSDSVLQKTLDYACGNGADCNPTHPKGSCFN 62

Query: 71  PNTIQAHASYAFNSFFQRKAMAAGSCDFAGAATIAQTDPXXXXXXXFDTAGGPITPTPPA 130
           P+ ++AH +YA NSFFQ+K  A+ SC+F G AT+  TDP         +A G       +
Sbjct: 63  PDNVRAHCNYAVNSFFQKKGQASESCNFTGTATLTTTDPSYTGCAFPSSASG-------S 122

Query: 131 NASPTITVPATTTSPNFNNGGTDDTNGLNPVFNPTST 168
           + S + TV     SP  +N  T    G +P     ST
Sbjct: 123 SGSGSTTVTPGKNSPKGSNSITTFPGGNSPYSGTPST 148

BLAST of MS007814 vs. TAIR 10
Match: AT5G61130.1 (plasmodesmata callose-binding protein 1 )

HSP 1 Score: 87.4 bits (215), Expect = 1.4e-17
Identity = 69/191 (36.13%), Postives = 97/191 (50.79%), Query Frame = 0

Query: 10  MPTLLSSFLLLVIGSSAVADGAYWCVVRSDASDSALQSALDYACATGAECAPILSNGLCF 69
           M  L+ S LLL   S A    A WCV ++  SD+ LQ+ LDYAC  GA+C P      CF
Sbjct: 1   MAALVLSLLLL---SLAGHSSASWCVCKTGLSDTVLQATLDYACGNGADCNPTKPKQSCF 60

Query: 70  LPNTIQAHASYAFNSFFQRKAMAAGSCDFAGAATIAQTDPXXXXXXXFDTAGG-----PI 129
            P+ +++H +YA NSFFQ+K  + GSC+F G AT   +DP         +A G      +
Sbjct: 61  NPDNVRSHCNYAVNSFFQKKGQSPGSCNFDGTATPTNSDPSYTGCAFPTSASGSSGSTTV 120

Query: 130 TP--TPPANASPTITVPATTTSPNFNNGGTDDTNGLNPVFNPTST-TTPTNSKASSSFTI 189
           TP  T P  +  T T+P + T+  ++   T+   G N     T T   P  +  SS+F +
Sbjct: 121 TPGTTNPKGSPTTTTLPGSGTNSPYSGNPTNGVFGGNSTGGTTGTGINPDYTTDSSAFAL 180

BLAST of MS007814 vs. TAIR 10
Match: AT1G13830.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 86.3 bits (212), Expect = 3.1e-17
Identity = 64/185 (34.59%), Postives = 92/185 (49.73%), Query Frame = 0

Query: 10  MPTLLSSFLLLVIGSSAVADGAYWCVVRSDASDSALQSALDYACATGAECAPILSNGLCF 69
           M  LL   LLL + +S+    A +C+ +    D+ LQ+++DYAC T A+C PI   G C+
Sbjct: 1   MKVLLGLLLLLSLTNSS---SAIYCLCKDGIGDTELQTSIDYACGTLADCNPIHDKGTCY 60

Query: 70  LPNTIQAHASYAFNSFFQRKAMAAGSCDFAGAATIAQTDPXXXXXXXFDTAGGPITPTPP 129
            P+TI++H  +A NS+FQ  A   GSC+F+G AT     P          +    T +PP
Sbjct: 61  QPDTIKSHCDWAVNSYFQNAAQVPGSCNFSGTATTNPNPPSNLANGCIYPSSPSSTRSPP 120

Query: 130 ANASPTITVP--ATTTSPNFNNGGTDDTNGLNPVFNPTSTTTPTNSKASSSFTIPKLLIF 189
           +   PT T P   TT  P     GT    G  PVF PT    P+N  + +S      +  
Sbjct: 121 STTPPTGTTPTNGTTPFPGTPFPGT-PFPGTPPVFGPTGVFNPSNPGSGASSLGTSSVFT 180

Query: 190 VLFSI 193
           + FS+
Sbjct: 181 LCFSL 181

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038875835.14.2e-6473.63PLASMODESMATA CALLOSE-BINDING PROTEIN 3 [Benincasa hispida][more]
XP_008457240.17.9e-6372.87PREDICTED: PLASMODESMATA CALLOSE-BINDING PROTEIN 3 [Cucumis melo][more]
KAE8651770.12.5e-6172.34hypothetical protein Csa_006265 [Cucumis sativus][more]
XP_004138961.22.5e-6172.34PLASMODESMATA CALLOSE-BINDING PROTEIN 3 [Cucumis sativus][more]
XP_022959424.11.4e-5970.44PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like [Cucurbita moschata] >XP_023548894.... [more]
Match NameE-valueIdentityDescription
Q9FZ864.5e-2138.96PLASMODESMATA CALLOSE-BINDING PROTEIN 3 OS=Arabidopsis thaliana OX=3702 GN=PDCB3... [more]
Q9SD848.0e-1838.22PLASMODESMATA CALLOSE-BINDING PROTEIN 2 OS=Arabidopsis thaliana OX=3702 GN=PDCB2... [more]
Q84V392.0e-1650.00Major pollen allergen Ole e 10 OS=Olea europaea OX=4146 PE=1 SV=1[more]
Q9FNQ22.0e-1636.13PLASMODESMATA CALLOSE-BINDING PROTEIN 1 OS=Arabidopsis thaliana OX=3702 GN=PDCB1... [more]
Q93V727.0e-1435.23PLASMODESMATA CALLOSE-BINDING PROTEIN 4 OS=Arabidopsis thaliana OX=3702 GN=PDCB4... [more]
Match NameE-valueIdentityDescription
A0A1S3C4M33.8e-6372.87PLASMODESMATA CALLOSE-BINDING PROTEIN 3 OS=Cucumis melo OX=3656 GN=LOC103496965 ... [more]
A0A0A0LND41.2e-6172.34X8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G139290 PE=4 SV=... [more]
A0A6J1H8126.7e-6070.44PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1KVB12.0e-5969.46PLASMODESMATA CALLOSE-BINDING PROTEIN 3 OS=Cucurbita maxima OX=3661 GN=LOC111499... [more]
A0A6J1HKA92.2e-5567.01PLASMODESMATA CALLOSE-BINDING PROTEIN 1-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
Match NameE-valueIdentityDescription
AT4G13600.16.9e-3347.78Carbohydrate-binding X8 domain superfamily protein [more]
AT1G18650.13.2e-2238.96plasmodesmata callose-binding protein 3 [more]
AT5G08000.15.7e-1938.22glucan endo-1,3-beta-glucosidase-like protein 3 [more]
AT5G61130.11.4e-1736.13plasmodesmata callose-binding protein 1 [more]
AT1G13830.13.1e-1734.59Carbohydrate-binding X8 domain superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 32..113
e-value: 1.2E-32
score: 124.5
IPR012946X8 domainPFAMPF07983X8coord: 33..103
e-value: 6.4E-20
score: 71.6
NoneNo IPR availableGENE3D1.20.58.1040coord: 25..114
e-value: 6.2E-11
score: 44.6
NoneNo IPR availablePANTHERPTHR31044:SF47CARBOHYDRATE-BINDING X8 DOMAIN SUPERFAMILY PROTEINcoord: 12..172
IPR044788Carbohydrate-binding X8 domain-containing protein, plantPANTHERPTHR31044BETA-1,3 GLUCANASEcoord: 12..172

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS007814.1MS007814.1mRNA