MS006005 (gene) Bitter gourd (TR) v1

Overview
NameMS006005
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionthermospermine synthase ACAULIS5-like
Locationscaffold254: 3070074 .. 3072605 (+)
RNA-Seq ExpressionMS006005
SyntenyMS006005
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGATGTTTCCTCTGTCAATTTGGTTAACAATGGAAATGAGCTCATTGAGAAGGGCCATTCCCTAAATGGGTATCGAAAGAGCTGCTGGTACGAGGAAGAGATTGAGGAGAATTTAAGATGGTCATTTGCTCTAAATAGGTACTTGTTTCTATTACATTTTAAATGAACTTTGTCCCACAAGAAAAAAAAAAAAAACGTGTCCTGTTTTCTGAATCCTCTCTGCCCATGTTCACCAATGTTTTCAACTTCATTCAGCTTTGTTTTCTAAAATTTTTCCCATTTCTAGAAGGAAGGTACAGCAAAAATGAGGGGGTCTTGAGGTAAAAGTGTGTTGGTATTTCGATTAGATTAAGGGGGAGTTGTTGAGACAAAATCATTAAACTATGCTTTCATAGTTCTAGCTTGTCTGTCGGTTCTTATATTGGGGATGTTTTGCTGGTCTTTGTGAAGGTCCAACAAGTTGAAATGGGGTTGAAATGTTTAAAAAAGTGGAAGACTTTTTCCCCTCCCACTTCATAATCTATCTCAATAAAATAATCCTTTGGTCTTGGAAAAGCCAGACAAGAAACCTTAAAAGTTAAAACAAAAAGGGGTTAAGTTTGGGCGTTATTTTTCACTTCCTGTCTGCCAAAATCTACAGGCTTTTTAATGAACTTTGTTTTGTAATCTTGTAGCATACTGCATACTGGAGCTTCTCAGTACCAAGACATTGCTCTACTCGACACGAAGCCGTTCGGGAAGGCGCTCGTCATCGATGGAAAGCTTCAAAGTGCAGAAACTGATGAATTCATTTACCATGAATGCCTTGTTCACCCTGCACTTCTCCATCATCCCAAGTATTTTCTAAGCCTAATTATTGATTCTCTTTGAGGAATTTAAGGATAAAACGATGTTGATAACATTCTTTTTGTAATGTCAGTCCAAAGACCATCTTTATCATGGGAGGAGGTGAAGGCTCCACAGCAAGAGAGCTACTGAGACACAAGACTGTGGAGAAAGTTGTCATGTGCGACATCGACGAGGTTCGAATTCCTTCGTTATTCTTCTTTATCGCCATTAATAGCTACGAAAGAAAGCTCTCTGAAACTGTCGTGGAATAAACTATTAAACAGGAGGTGGTCGACTTCTGCAAGTCGTATCTGATAGTAAACAAAGAAGCTTTCTGCGATCCAAGACTTGAACTTGTCATCAACGATGCCAGGTTCGGGTTCGATTTAAGGCATTTTTACGATTCATCCTACATTACAGTTGCTATTTTCAGTTACTAAAAGTTTTCTGGTGGGCATGTCTGCAGAGCGGAGCTCGAGAGTCGAGAAGAGCAATACGACGTGATTGTGGGCGACCTTGCAGACCCAATAGAGGGAGGTCCTTGTTACAAACTCTACACCAAATCCTTCTATGAGTATACTGTGAAGCCTAGATTGAAACAAGATGGCATATTCATCACTCAGGTAAGCAAAAGAGATGAAAATGAATTCAGTTTTTTGAATGTCAAATGGGAGAGGGATGAATTCAAATTGTTGAAATCTATGCAGGCTGGTCCAGCTGGAATCTTCAGCCATACAGAAGTGTTTTCTTGCATCTACAATACTCTAAAGCAAGTTTTCAAATGTGAGCAAAACCTGAGGCTATTTCTGTTGTTGGTTTTATCCTAAGTTTCTATCAGGAATTTACTAATGCATAGAGTGGTGGTTCTGTTCTTTTTTTTTCCTTTTTCTTTCTTATTTGCAGATGTGGTGCCTTATTCTGCCCATATTCCTTCCTTTGCTGATACTTGGGGTTGGGTTATGGTAAGAAAATTATACTTTCTCATGCTTTTGGACTGGATTAATGATTATCTTTTTCATAATGATAAAACATGAGATGAAAGGTCTAGAGGCTGTTTGGTGTGTTTTATACCGAATCGTTTTTCAAGGTTGTTGGAAAATTTCTAGTACTACGGGAGTATCTACTCCTTTCTTTTATAAATAATTCTTTAATGTTGTTGGACCCCACGTCATTCTTAAATGATGCTGACGTAGCTAGCCATGAAGAAGAGTAATATCCATATTCTTGTAGTATTATTTATCGAAAAGAAACCGTTCATCCATGATAACAAAAGTAGGGAAAATCATTGTCCAAGCGTGTTTTTCACTTAAATGAAAACGACAACGGTTTCTAAACGCCACTTTCAAATGGGGGTTCTAGTTTCTTTTTTTAAGAAAGATTTCTTGAAACTTGCAGGCTTCAGACACCCCTTTTGTCCTGAATGCTGATGATTTGGACCAGAGAATCAAACAAAGAATCAAAGGGGAGAACAAGTACTTTGATGGAAAAACATTCTCATCTGCATCCACATTGAGCAAAGCTGTTCGAAAGTCGTAAGTAAACGTTTTTTTTTTCCTTCTGATTTATTTACAAAATTCTGAAAAGTTCTAAATCAGGCAAGAATTTTCGCAGGCTCGACACCGAGACTCACGTGTACACGGAAGGAACAGCGAGGTTCATATATGGATATGGAGCTGCAGCCTGCAAGCTCAACCAAGTT

mRNA sequence

ATGGCCGATGTTTCCTCTGTCAATTTGGTTAACAATGGAAATGAGCTCATTGAGAAGGGCCATTCCCTAAATGGGTATCGAAAGAGCTGCTGGTACGAGGAAGAGATTGAGGAGAATTTAAGATGGTCATTTGCTCTAAATAGCATACTGCATACTGGAGCTTCTCAGTACCAAGACATTGCTCTACTCGACACGAAGCCGTTCGGGAAGGCGCTCGTCATCGATGGAAAGCTTCAAAGTGCAGAAACTGATGAATTCATTTACCATGAATGCCTTGTTCACCCTGCACTTCTCCATCATCCCAATCCAAAGACCATCTTTATCATGGGAGGAGGTGAAGGCTCCACAGCAAGAGAGCTACTGAGACACAAGACTGTGGAGAAAGTTGTCATGTGCGACATCGACGAGGAGGTGGTCGACTTCTGCAAGTCGTATCTGATAGTAAACAAAGAAGCTTTCTGCGATCCAAGACTTGAACTTGTCATCAACGATGCCAGAGCGGAGCTCGAGAGTCGAGAAGAGCAATACGACGTGATTGTGGGCGACCTTGCAGACCCAATAGAGGGAGGTCCTTGTTACAAACTCTACACCAAATCCTTCTATGAGTATACTGTGAAGCCTAGATTGAAACAAGATGGCATATTCATCACTCAGGCTGGTCCAGCTGGAATCTTCAGCCATACAGAAGTGTTTTCTTGCATCTACAATACTCTAAAGCAAGTTTTCAAATATGTGGTGCCTTATTCTGCCCATATTCCTTCCTTTGCTGATACTTGGGGTTGGGTTATGGCTTCAGACACCCCTTTTGTCCTGAATGCTGATGATTTGGACCAGAGAATCAAACAAAGAATCAAAGGGGAGAACAAGTACTTTGATGGAAAAACATTCTCATCTGCATCCACATTGAGCAAAGCTGTTCGAAAGTCGCTCGACACCGAGACTCACGTGTACACGGAAGGAACAGCGAGGTTCATATATGGATATGGAGCTGCAGCCTGCAAGCTCAACCAAGTT

Coding sequence (CDS)

ATGGCCGATGTTTCCTCTGTCAATTTGGTTAACAATGGAAATGAGCTCATTGAGAAGGGCCATTCCCTAAATGGGTATCGAAAGAGCTGCTGGTACGAGGAAGAGATTGAGGAGAATTTAAGATGGTCATTTGCTCTAAATAGCATACTGCATACTGGAGCTTCTCAGTACCAAGACATTGCTCTACTCGACACGAAGCCGTTCGGGAAGGCGCTCGTCATCGATGGAAAGCTTCAAAGTGCAGAAACTGATGAATTCATTTACCATGAATGCCTTGTTCACCCTGCACTTCTCCATCATCCCAATCCAAAGACCATCTTTATCATGGGAGGAGGTGAAGGCTCCACAGCAAGAGAGCTACTGAGACACAAGACTGTGGAGAAAGTTGTCATGTGCGACATCGACGAGGAGGTGGTCGACTTCTGCAAGTCGTATCTGATAGTAAACAAAGAAGCTTTCTGCGATCCAAGACTTGAACTTGTCATCAACGATGCCAGAGCGGAGCTCGAGAGTCGAGAAGAGCAATACGACGTGATTGTGGGCGACCTTGCAGACCCAATAGAGGGAGGTCCTTGTTACAAACTCTACACCAAATCCTTCTATGAGTATACTGTGAAGCCTAGATTGAAACAAGATGGCATATTCATCACTCAGGCTGGTCCAGCTGGAATCTTCAGCCATACAGAAGTGTTTTCTTGCATCTACAATACTCTAAAGCAAGTTTTCAAATATGTGGTGCCTTATTCTGCCCATATTCCTTCCTTTGCTGATACTTGGGGTTGGGTTATGGCTTCAGACACCCCTTTTGTCCTGAATGCTGATGATTTGGACCAGAGAATCAAACAAAGAATCAAAGGGGAGAACAAGTACTTTGATGGAAAAACATTCTCATCTGCATCCACATTGAGCAAAGCTGTTCGAAAGTCGCTCGACACCGAGACTCACGTGTACACGGAAGGAACAGCGAGGTTCATATATGGATATGGAGCTGCAGCCTGCAAGCTCAACCAAGTT

Protein sequence

MADVSSVNLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELVINDARAELESREEQYDVIVGDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQVFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSASTLSKAVRKSLDTETHVYTEGTARFIYGYGAAACKLNQV
Homology
[Close]
BLAST of MS006005 vs. NCBI nr
Match: XP_022138299.1 (thermospermine synthase ACAULIS5-like [Momordica charantia])

HSP 1 Score: 699.1 bits (1803), Expect = 1.8e-197
Identity = 337/338 (99.70%), Postives = 338/338 (100.00%), Query Frame = 0 

Query: 1   MADVSSVNLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSVNLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSVNLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELVINDARAELESREEQYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELVINDARAELESREEQYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELVINDARAELESREEQYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMAS+TPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASNTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDTETHVYTEGTARFIYGYGAAACKLNQV 339
           TLSKAVRKSLDTETHVYTEGTARFIYGYGAAACKLNQV
Sbjct: 301 TLSKAVRKSLDTETHVYTEGTARFIYGYGAAACKLNQV 338

[Close]
BLAST of MS006005 vs. NCBI nr
Match: XP_038880186.1 (thermospermine synthase ACAULIS5-like [Benincasa hispida])

HSP 1 Score: 686.4 bits (1770), Expect = 1.2e-193
Identity = 330/338 (97.63%), Postives = 332/338 (98.22%), Query Frame = 0 

Query: 1   MADVSSVNLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSS NLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELVINDARAELESREEQYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLEL+INDARAELE+R E YDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRI GENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRITGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDTETHVYTEGTARFIYGYGAAACKLNQV 339
            LSKAVRKSLD ETHVYTEGTARFIYGYGAAACKLNQV
Sbjct: 301 ILSKAVRKSLDNETHVYTEGTARFIYGYGAAACKLNQV 338

[Close]
BLAST of MS006005 vs. NCBI nr
Match: KAG6597450.1 (Thermospermine synthase ACAULIS5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 682.9 bits (1761), Expect = 1.4e-192
Identity = 330/339 (97.35%), Postives = 334/339 (98.53%), Query Frame = 0 

Query: 1   MADVSSVNLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSS NLVNNGNELIEKGH+LNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHP+LLHHPNPKTIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELVINDARAELESREEQYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAF DPRLEL+INDARAELE+R EQYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDTETHVYTEGTARFIYGYG-AAACKLNQV 339
           TLSKAVRKSLD ETHVYTEGTARFIYGYG AAACKLNQV
Sbjct: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV 339

[Close]
BLAST of MS006005 vs. NCBI nr
Match: XP_023539341.1 (thermospermine synthase ACAULIS5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 682.2 bits (1759), Expect = 2.3e-192
Identity = 329/339 (97.05%), Postives = 334/339 (98.53%), Query Frame = 0 

Query: 1   MADVSSVNLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSS NLVNNGNELIEKGH+LNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHP+LLHHPNP+TIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPRTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELVINDARAELESREEQYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAF DPRLEL+INDARAELE+R EQYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRSEQYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDTETHVYTEGTARFIYGYG-AAACKLNQV 339
           TLSKAVRKSLD ETHVYTEGTARFIYGYG AAACKLNQV
Sbjct: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV 339

[Close]
BLAST of MS006005 vs. NCBI nr
Match: XP_022934423.1 (thermospermine synthase ACAULIS5-like [Cucurbita moschata])

HSP 1 Score: 681.0 bits (1756), Expect = 5.2e-192
Identity = 329/339 (97.05%), Postives = 334/339 (98.53%), Query Frame = 0 

Query: 1   MADVSSVNLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSS NLVNNGNELIEKGH+LNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHP+LLHHPNPKTIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELVINDARAELESREEQYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAF DPRLEL+INDARAELE+R EQYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYE+TVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEHTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDTETHVYTEGTARFIYGYG-AAACKLNQV 339
           TLSKAVRKSLD ETHVYTEGTARFIYGYG AAACKLNQV
Sbjct: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV 339

[Close]
BLAST of MS006005 vs. ExPASy Swiss-Prot
Match: Q9S7X6 (Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 3.4e-130
Identity = 214/328 (65.24%), Postives = 269/328 (82.01%), Query Frame = 0 

Query: 9   LVNNGNELIEKGHS----LNGYRKSC-WYEEEIEENLRWSFALNSILHTGASQYQDIALL 68
           +  NG   I K  S    L+  ++ C WYEE I+++L+WSFALNS+LH G S+YQDIALL
Sbjct: 8   MFGNGFPEIHKATSPTQTLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALL 67

Query: 69  DTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTARELLRH 128
           DTK FGK LVIDGK+QSAE DEFIYHECL+HPALL HPNPKT+FIMGGGEGS ARE+L+H
Sbjct: 68  DTKRFGKVLVIDGKMQSAERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKH 127

Query: 129 KTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELVINDARAELESREEQYDVIVGDL 188
            T+EKVVMCDID+EVVDFC+ +L VN +AFC+ +LELVI DA+AELE REE++D+IVGDL
Sbjct: 128 TTIEKVVMCDIDQEVVDFCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDL 187

Query: 189 ADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQVFK 248
           ADP+EGGPCY+LYTKSFY+  +KP+L  +GIF+TQAGPAGIF+H EVF+ IYNT+KQVFK
Sbjct: 188 ADPVEGGPCYQLYTKSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFK 247

Query: 249 YVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSASTLS 308
           YV  Y+AH+PSFADTWGWVMASD  F +  D++D+RI++R+ GE  Y +  +F SA+TL+
Sbjct: 248 YVKAYTAHVPSFADTWGWVMASDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLN 307

Query: 309 KAVRKSLDTETHVYTEGTARFIYGYGAA 332
           K +  +L+ ET VY+E  ARFI+G+G A
Sbjct: 308 KTISLALEKETEVYSEENARFIHGHGVA 335

[Close]
BLAST of MS006005 vs. ExPASy Swiss-Prot
Match: Q975S5 (Polyamine aminopropyltransferase OS=Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) OX=273063 GN=speE PE=3 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 6.0e-58
Identity = 119/277 (42.96%), Postives = 173/277 (62.45%), Query Frame = 0 

Query: 45  ALNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPK 104
           A+  ++    + YQ   L++   FGKAL++DGK+QS  TDEFIYHE LVHP LL   NP+
Sbjct: 20  AITKVIAEEKTPYQRALLVELARFGKALILDGKIQSTITDEFIYHEALVHPLLLSINNPE 79

Query: 105 TIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIV-NKEAFCDPRLELVIN 164
            I I+GGGEG+T RE+L+HKT++ V M DID  V+DF K YL   ++ AF +P+ +LVI 
Sbjct: 80  KILILGGGEGATLREVLKHKTIKNVTMVDIDPVVIDFAKKYLQEWHQGAFDNPKSKLVIE 139

Query: 165 DARAELESREEQYDVIVGDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAG 224
           D    ++  +E++D +V DL DPI+  P   LYTK FYE     R+ + GI      P+ 
Sbjct: 140 DGYKFIKETKEKFDAVVIDLTDPIKDSPSQMLYTKEFYEEV--KRISKWGIVTQATSPS- 199

Query: 225 IFSHTEVFSCIYNTLKQVFKYVVPYSAHIPSFADTWGWVMASD--TPFVLNADDLDQRIK 284
            FS  E FS IYNT+K VFK V     ++P+F   WG+V ASD   P   + ++++ RIK
Sbjct: 200 -FS-LETFSIIYNTIKHVFKKVSAGITYVPAFDGLWGFVYASDEVNPAEFSKEEINNRIK 259

Query: 285 QRIKGENKYFDGKTFSSASTLSKAVRKSLDTETHVYT 319
           +RI G  +++DG+T  +  ++ K +R+ L+ E  + T
Sbjct: 260 ERIDGSLRFYDGETHITMFSIPKHIREVLEKENKIST 291

[Close]
BLAST of MS006005 vs. ExPASy Swiss-Prot
Match: A2BJU2 (Polyamine aminopropyltransferase 2 OS=Hyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5) OX=415426 GN=speE2 PE=1 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 7.3e-56
Identity = 120/272 (44.12%), Postives = 169/272 (62.13%), Query Frame = 0 

Query: 49  ILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFI 108
           I   GA++YQ+  + +    GKALV+DGK+QS+  DE  YHE LVHP LL H  P+ + +
Sbjct: 27  IYAAGATRYQEYMIAELAGIGKALVLDGKVQSSILDEHWYHEALVHPILLAHQCPRKVLV 86

Query: 109 MGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIV-NKEAFCDPRLELVINDARA 168
           +GGGEG+T RE+LRH  VE V M DIDEE+V+  K +L   ++ AF   +LELVI D R 
Sbjct: 87  IGGGEGATVREVLRHSCVEHVTMVDIDEELVELAKKHLAEWHQGAFSSDKLELVIGDGRR 146

Query: 169 ELESREEQYDVIVGDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSH 228
            +E+   +YD I+ DL DP+EGGP  +LYT  FY   VK  L+  G  +TQA    +   
Sbjct: 147 YVENCHRKYDAIILDLVDPMEGGPAARLYTLEFYR-AVKGCLRPGGAVVTQATSPTL--S 206

Query: 229 TEVFSCIYNTLKQVFKYVVPYSAHIPSFADTWGWVMASDT--PFVLNADDLDQRIKQRIK 288
             V++ I NTL +VF  V PY +++ S+   WG+V ASDT  P  L+A ++D+ I  RI+
Sbjct: 207 PRVYAVIRNTLAKVFTIVRPYVSYVRSYNGLWGFVAASDTVDPAKLSAKEVDELIAARIR 266

Query: 289 GENKYFDGKTFSSASTLSKAVRKSLDTETHVY 318
           G+ +++DG+T      L   VR+ L +ET  Y
Sbjct: 267 GQLRFYDGETHEWMFRLPLPVRQVL-SETRDY 294

[Close]
BLAST of MS006005 vs. ExPASy Swiss-Prot
Match: Q9UXE4 (Polyamine aminopropyltransferase OS=Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) OX=273057 GN=speE PE=1 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 2.3e-54
Identity = 113/276 (40.94%), Postives = 166/276 (60.14%), Query Frame = 0 

Query: 46  LNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPN-PK 105
           +  +L    + YQ + L++   FGK L+IDGK+QS   DE IYHE LVHP LL     PK
Sbjct: 21  IEKVLAEEKTPYQHVTLVEFTRFGKGLIIDGKVQSTLYDEHIYHELLVHPLLLSLTKPPK 80

Query: 106 TIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIV-NKEAFCDPRLELVIN 165
           ++ I+GGGEG+T RE+L++K+VEK VM DIDE+V++F K YL   ++ AF D R  LVI 
Sbjct: 81  SVLILGGGEGATLREVLKYKSVEKAVMVDIDEKVIEFAKKYLYEWHQGAFEDKRTNLVIT 140

Query: 166 DARAELESREEQYDVIVGDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAG 225
           D    ++  +E+YD I+ DL DPI+    Y LYTK FYE  ++  L + G  +TQA    
Sbjct: 141 DGLKFIKETKEKYDAIILDLTDPIKNSTSYMLYTKEFYE-KLREILNERGGIVTQATSPS 200

Query: 226 IFSHTEVFSCIYNTLKQVFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQR 285
                EV+  IYNT+K+VFK       ++ SF   WG+V     P +L+ D++D +I +R
Sbjct: 201 F--SLEVYVTIYNTIKEVFKEASASYTYMASFDGLWGFVYGGVRPDLLSEDEVDSKINER 260

Query: 286 IKGENKYFDGKTFSSASTLSKAVRKSLDTETHVYTE 320
           I G+ +++DG +   + +L K ++      T + TE
Sbjct: 261 IDGQLRFYDGYSHKISFSLPKNIKSEFQKITKISTE 293

[Close]
BLAST of MS006005 vs. ExPASy Swiss-Prot
Match: Q9YE02 (Polyamine aminopropyltransferase OS=Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) OX=272557 GN=speE PE=3 SV=2)

HSP 1 Score: 213.4 bits (542), Expect = 4.0e-54
Identity = 116/305 (38.03%), Postives = 186/305 (60.98%), Query Frame = 0 

Query: 20  GHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDIALLDTKPFGKALVIDGKLQ 79
           G   + + K  W+ E +  +      +  ++  G S++Q+IA++     GK LV+DGK Q
Sbjct: 4   GSEKSVFLKWSWFLEWLTPDRATLKHIEDVIFQGRSRFQEIAVVRVSGEGKVLVLDGKTQ 63

Query: 80  SAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVV 139
           S+E+DEF+YHE LVHPA++ H +P+ + I+GGGEG+T RE+L+H++VEK VM DIDE VV
Sbjct: 64  SSESDEFMYHEALVHPAMILHGSPRKVLILGGGEGATLREVLKHRSVEKAVMVDIDETVV 123

Query: 140 DFCKSYLIV-NKEAFCDPRLELVINDARAELESREEQ-YDVIVGDLADPIEGGPCYKLYT 199
           +  + +L   ++ AF DPR E+VI+DA   + S+ E  +DV++ DL DP+E GP  +LY+
Sbjct: 124 NVAREHLREWHRGAFDDPRAEVVIDDAWNYVASKAETGFDVVIADLVDPLEAGPATRLYS 183

Query: 200 KSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQVFKYVVPYSAHIPSFAD 259
           + +Y   VK  +   G+F+TQA    I   TE  + I NT+ +VFK+V  Y  ++PSF  
Sbjct: 184 EEYYR-MVKDVMNPGGVFVTQA--VSISHLTEYHAIIRNTVARVFKHVESYGVYVPSFDS 243

Query: 260 TWGWVMASD--TPFVL-NADDLDQRIKQRIKG-ENKYFDGKTFSSASTLSKAVRKSLDTE 319
            WG+V+ASD   P +L +    + R+  +++G E ++ D  +      + K  R+++  E
Sbjct: 244 MWGFVVASDDKDPRILGDRGFFETRLSHQLQGAELRFLDYASMLHMLNIPKMYREAIARE 303

[Close]
BLAST of MS006005 vs. ExPASy TrEMBL
Match: A0A6J1C9S2 (thermospermine synthase ACAULIS5-like OS=Momordica charantia OX=3673 GN=LOC111009509 PE=3 SV=1)

HSP 1 Score: 699.1 bits (1803), Expect = 8.9e-198
Identity = 337/338 (99.70%), Postives = 338/338 (100.00%), Query Frame = 0 

Query: 1   MADVSSVNLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSVNLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSVNLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELVINDARAELESREEQYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELVINDARAELESREEQYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELVINDARAELESREEQYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMAS+TPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASNTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDTETHVYTEGTARFIYGYGAAACKLNQV 339
           TLSKAVRKSLDTETHVYTEGTARFIYGYGAAACKLNQV
Sbjct: 301 TLSKAVRKSLDTETHVYTEGTARFIYGYGAAACKLNQV 338

[Close]
BLAST of MS006005 vs. ExPASy TrEMBL
Match: A0A6J1F7M2 (thermospermine synthase ACAULIS5-like OS=Cucurbita moschata OX=3662 GN=LOC111441602 PE=3 SV=1)

HSP 1 Score: 681.0 bits (1756), Expect = 2.5e-192
Identity = 329/339 (97.05%), Postives = 334/339 (98.53%), Query Frame = 0 

Query: 1   MADVSSVNLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSS NLVNNGNELIEKGH+LNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHP+LLHHPNPKTIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELVINDARAELESREEQYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAF DPRLEL+INDARAELE+R EQYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYE+TVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEHTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDTETHVYTEGTARFIYGYG-AAACKLNQV 339
           TLSKAVRKSLD ETHVYTEGTARFIYGYG AAACKLNQV
Sbjct: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV 339

[Close]
BLAST of MS006005 vs. ExPASy TrEMBL
Match: A0A6J1IJL1 (thermospermine synthase ACAULIS5-like OS=Cucurbita maxima OX=3661 GN=LOC111474176 PE=3 SV=1)

HSP 1 Score: 679.9 bits (1753), Expect = 5.6e-192
Identity = 329/339 (97.05%), Postives = 333/339 (98.23%), Query Frame = 0 

Query: 1   MADVSSVNLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSS NLVNNGNELIEKGH+LNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHP+LLHHPNPKTIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELVINDARAELESREEQYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAF DPRLEL+INDARAELE+R EQYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIK ENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKEENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDTETHVYTEGTARFIYGYG-AAACKLNQV 339
           TLSKAVRKSLD ETHVYTEGTARFIYGYG AAACKLNQV
Sbjct: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV 339

[Close]
BLAST of MS006005 vs. ExPASy TrEMBL
Match: A0A0A0L7M9 (Spermine synthase OS=Cucumis sativus OX=3659 GN=Csa_3G147770 PE=3 SV=1)

HSP 1 Score: 676.4 bits (1744), Expect = 6.2e-191
Identity = 326/338 (96.45%), Postives = 329/338 (97.34%), Query Frame = 0 

Query: 1   MADVSSVNLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSS NLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPK+IFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELVINDARAELESREEQYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLEL+INDARAELE+R E YDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPF LNADDLDQRIKQRI GENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDTETHVYTEGTARFIYGYGAAACKLNQV 339
           TLSKAVRKSLD ET VYTE TARFIYGYGA ACKLNQV
Sbjct: 301 TLSKAVRKSLDNETQVYTEETARFIYGYGAEACKLNQV 338

[Close]
BLAST of MS006005 vs. ExPASy TrEMBL
Match: A0A5D3D0P3 (Thermospermine synthase ACAULIS5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00350 PE=3 SV=1)

HSP 1 Score: 675.2 bits (1741), Expect = 1.4e-190
Identity = 325/338 (96.15%), Postives = 329/338 (97.34%), Query Frame = 0 

Query: 1   MADVSSVNLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSS NLVNNGNELIEKGH+LNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHALNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPK+IFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELVINDARAELESREEQYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLEL+INDARAELE+R E YDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPF LNADDLDQRIKQRI GENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDTETHVYTEGTARFIYGYGAAACKLNQV 339
           TLSKAVRKSLD ET VYTE TARFIYGYGA ACKLNQV
Sbjct: 301 TLSKAVRKSLDNETQVYTEETARFIYGYGAEACKLNQV 338

[Close]
BLAST of MS006005 vs. TAIR 10
Match: AT5G19530.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 466.1 bits (1198), Expect = 2.4e-131
Identity = 214/328 (65.24%), Postives = 269/328 (82.01%), Query Frame = 0 

Query: 9   LVNNGNELIEKGHS----LNGYRKSC-WYEEEIEENLRWSFALNSILHTGASQYQDIALL 68
           +  NG   I K  S    L+  ++ C WYEE I+++L+WSFALNS+LH G S+YQDIALL
Sbjct: 8   MFGNGFPEIHKATSPTQTLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALL 67

Query: 69  DTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTARELLRH 128
           DTK FGK LVIDGK+QSAE DEFIYHECL+HPALL HPNPKT+FIMGGGEGS ARE+L+H
Sbjct: 68  DTKRFGKVLVIDGKMQSAERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKH 127

Query: 129 KTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELVINDARAELESREEQYDVIVGDL 188
            T+EKVVMCDID+EVVDFC+ +L VN +AFC+ +LELVI DA+AELE REE++D+IVGDL
Sbjct: 128 TTIEKVVMCDIDQEVVDFCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDL 187

Query: 189 ADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQVFK 248
           ADP+EGGPCY+LYTKSFY+  +KP+L  +GIF+TQAGPAGIF+H EVF+ IYNT+KQVFK
Sbjct: 188 ADPVEGGPCYQLYTKSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFK 247

Query: 249 YVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSASTLS 308
           YV  Y+AH+PSFADTWGWVMASD  F +  D++D+RI++R+ GE  Y +  +F SA+TL+
Sbjct: 248 YVKAYTAHVPSFADTWGWVMASDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLN 307

Query: 309 KAVRKSLDTETHVYTEGTARFIYGYGAA 332
           K +  +L+ ET VY+E  ARFI+G+G A
Sbjct: 308 KTISLALEKETEVYSEENARFIHGHGVA 335

[Close]
BLAST of MS006005 vs. TAIR 10
Match: AT5G19530.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 466.1 bits (1198), Expect = 2.4e-131
Identity = 214/328 (65.24%), Postives = 269/328 (82.01%), Query Frame = 0 

Query: 9   LVNNGNELIEKGHS----LNGYRKSC-WYEEEIEENLRWSFALNSILHTGASQYQDIALL 68
           +  NG   I K  S    L+  ++ C WYEE I+++L+WSFALNS+LH G S+YQDIALL
Sbjct: 1   MFGNGFPEIHKATSPTQTLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALL 60

Query: 69  DTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTARELLRH 128
           DTK FGK LVIDGK+QSAE DEFIYHECL+HPALL HPNPKT+FIMGGGEGS ARE+L+H
Sbjct: 61  DTKRFGKVLVIDGKMQSAERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKH 120

Query: 129 KTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELVINDARAELESREEQYDVIVGDL 188
            T+EKVVMCDID+EVVDFC+ +L VN +AFC+ +LELVI DA+AELE REE++D+IVGDL
Sbjct: 121 TTIEKVVMCDIDQEVVDFCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDL 180

Query: 189 ADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQVFK 248
           ADP+EGGPCY+LYTKSFY+  +KP+L  +GIF+TQAGPAGIF+H EVF+ IYNT+KQVFK
Sbjct: 181 ADPVEGGPCYQLYTKSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFK 240

Query: 249 YVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSASTLS 308
           YV  Y+AH+PSFADTWGWVMASD  F +  D++D+RI++R+ GE  Y +  +F SA+TL+
Sbjct: 241 YVKAYTAHVPSFADTWGWVMASDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLN 300

Query: 309 KAVRKSLDTETHVYTEGTARFIYGYGAA 332
           K +  +L+ ET VY+E  ARFI+G+G A
Sbjct: 301 KTISLALEKETEVYSEENARFIHGHGVA 328

[Close]
BLAST of MS006005 vs. TAIR 10
Match: AT1G23820.2 (spermidine synthase 1 )

HSP 1 Score: 154.1 bits (388), Expect = 2.0e-37
Identity = 80/225 (35.56%), Postives = 128/225 (56.89%), Query Frame = 0 

Query: 43  SFALNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPN 102
           S  +  +L  G S YQD+ +  +  +GK LV+DG +Q  E DE  Y E + H  L   PN
Sbjct: 105 SLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPN 164

Query: 103 PKTIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELVI 162
           PK + ++GGG+G   RE+ RH ++E++ MC+ID+ VVD  K +       + DPR+ LVI
Sbjct: 165 PKKVLVIGGGDGGVLREVARHASIEQIDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVI 224

Query: 163 NDARAELESREE-QYDVIVGDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGP 222
            D  A L++  E  YD ++ D +DPI  GP  +L+ K F++ +V   L+  G+  TQA  
Sbjct: 225 GDGVAFLKNAAEGSYDAVIVDSSDPI--GPAKELFEKPFFQ-SVARALRPGGVVCTQA-- 284

Query: 223 AGIFSHTEVFSCIYNTLKQVFKYVVPYS-AHIPSFADTWG-WVMA 265
             ++ H ++   I +  +++FK  V Y+   +P++   WG W+ A
Sbjct: 285 ESLWLHMDIIEDIVSNCREIFKGSVNYAWTSVPTYPRHWGHWIYA 324

[Close]
BLAST of MS006005 vs. TAIR 10
Match: AT1G23820.1 (spermidine synthase 1 )

HSP 1 Score: 153.7 bits (387), Expect = 2.7e-37
Identity = 87/280 (31.07%), Postives = 153/280 (54.64%), Query Frame = 0 

Query: 43  SFALNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPN 102
           S  +  +L  G S YQD+ +  +  +GK LV+DG +Q  E DE  Y E + H  L   PN
Sbjct: 105 SLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPN 164

Query: 103 PKTIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELVI 162
           PK + ++GGG+G   RE+ RH ++E++ MC+ID+ VVD  K +       + DPR+ LVI
Sbjct: 165 PKKVLVIGGGDGGVLREVARHASIEQIDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVI 224

Query: 163 NDARAELESREE-QYDVIVGDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGP 222
            D  A L++  E  YD ++ D +DPI  GP  +L+ K F++ +V   L+  G+  TQA  
Sbjct: 225 GDGVAFLKNAAEGSYDAVIVDSSDPI--GPAKELFEKPFFQ-SVARALRPGGVVCTQA-- 284

Query: 223 AGIFSHTEVFSCIYNTLKQVFKYVVPYS-AHIPSF-ADTWGWVMAS----DTPFVLNADD 282
             ++ H ++   I +  +++FK  V Y+   +P++ +   G+++ S    D  F    + 
Sbjct: 285 ESLWLHMDIIEDIVSNCREIFKGSVNYAWTSVPTYPSGVIGFMLCSTEGPDVDFKHPLNP 344

Query: 283 LDQRIKQRIKGENKYFDGKTFSSASTLSKAVRKSLDTETH 316
           +D+    +  G  K+++ +  S+A  L    +K ++++ +
Sbjct: 345 IDES-SSKSNGPLKFYNAEIHSAAFCLPSFAKKVIESKAN 378

[Close]
BLAST of MS006005 vs. TAIR 10
Match: AT1G70310.1 (spermidine synthase 2 )

HSP 1 Score: 149.4 bits (376), Expect = 5.0e-36
Identity = 91/283 (32.16%), Postives = 151/283 (53.36%), Query Frame = 0 

Query: 43  SFALNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPN 102
           S  +  IL  G S YQD+ +  +  +GK LV+DG +Q  E DE  Y E + H  L    N
Sbjct: 65  SLKVEKILFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSISN 124

Query: 103 PKTIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELVI 162
           PK + ++GGG+G   RE+ RH +VE++ +C+ID+ VVD  K Y       + DPR+ L+I
Sbjct: 125 PKKVLVIGGGDGGVLREVARHSSVEQIDICEIDKMVVDVAKQYFPNVAVGYEDPRVNLII 184

Query: 163 NDARAELESREE-QYDVIVGDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGP 222
            D  A L++  E  YD ++ D +DPI  GP  +L+ K F+E +V   L+  G+  TQA  
Sbjct: 185 GDGVAFLKNAAEGTYDAVIVDSSDPI--GPAKELFEKPFFE-SVNRALRPGGVVCTQA-- 244

Query: 223 AGIFSHTEVFSCIYNTLKQVFKYVVPYS-AHIPSF-ADTWGWVMAS------DTPFVLNA 282
             ++ H ++   I +  + +FK  V Y+   +P++ +   G+++ S      D    ++ 
Sbjct: 245 ESLWLHMDIIEDIVSNCRDIFKGSVNYAWTSVPTYPSGVIGFMLCSSEGPQVDFKKPVSL 304

Query: 283 DDLDQ-RIKQRIKGENKYFDGKTFSSASTLSKAVRKSLDTETH 316
            D D+  IK       KY++ +  S+A  L    +K +D++ +
Sbjct: 305 IDTDESSIKSHC--PLKYYNAEIHSAAFCLPSFAKKVIDSKAN 340

The following BLAST results are available for this feature:
BLAST of MS006005 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022138299.11.8e-19799.70thermospermine synthase ACAULIS5-like [Momordica charantia][more]
XP_038880186.11.2e-19397.63thermospermine synthase ACAULIS5-like [Benincasa hispida][more]
KAG6597450.11.4e-19297.35Thermospermine synthase ACAULIS5, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_023539341.12.3e-19297.05thermospermine synthase ACAULIS5-like [Cucurbita pepo subsp. pepo][more]
XP_022934423.15.2e-19297.05thermospermine synthase ACAULIS5-like [Cucurbita moschata][more]
BLAST of MS006005 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9S7X63.4e-13065.24Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV... [more]
Q975S56.0e-5842.96Polyamine aminopropyltransferase OS=Sulfurisphaera tokodaii (strain DSM 16993 / ... [more]
A2BJU27.3e-5644.12Polyamine aminopropyltransferase 2 OS=Hyperthermus butylicus (strain DSM 5456 / ... [more]
Q9UXE42.3e-5440.94Polyamine aminopropyltransferase OS=Saccharolobus solfataricus (strain ATCC 3509... [more]
Q9YE024.0e-5438.03Polyamine aminopropyltransferase OS=Aeropyrum pernix (strain ATCC 700893 / DSM 1... [more]
BLAST of MS006005 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1C9S28.9e-19899.70thermospermine synthase ACAULIS5-like OS=Momordica charantia OX=3673 GN=LOC11100... [more]
A0A6J1F7M22.5e-19297.05thermospermine synthase ACAULIS5-like OS=Cucurbita moschata OX=3662 GN=LOC111441... [more]
A0A6J1IJL15.6e-19297.05thermospermine synthase ACAULIS5-like OS=Cucurbita maxima OX=3661 GN=LOC11147417... [more]
A0A0A0L7M96.2e-19196.45Spermine synthase OS=Cucumis sativus OX=3659 GN=Csa_3G147770 PE=3 SV=1[more]
A0A5D3D0P31.4e-19096.15Thermospermine synthase ACAULIS5-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
BLAST of MS006005 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G19530.12.4e-13165.24S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G19530.22.4e-13165.24S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G23820.22.0e-3735.56spermidine synthase 1 [more]
AT1G23820.12.7e-3731.07spermidine synthase 1 [more]
AT1G70310.15.0e-3632.16spermidine synthase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR037163Spermidine synthase, tetramerisation domain superfamilyGENE3D2.30.140.10Spermidine synthase, tetramerisation domaincoord: 29..81
e-value: 7.1E-15
score: 56.8
IPR035246Spermidine synthase, tetramerisation domainPFAMPF17284Spermine_synt_Ncoord: 31..82
e-value: 2.5E-13
score: 49.7
NoneNo IPR availablePFAMPF01564Spermine_synthcoord: 85..262
e-value: 1.2E-42
score: 145.5
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 82..334
e-value: 2.5E-77
score: 261.8
NoneNo IPR availablePANTHERPTHR43317:SF9SPERMINE SYNTHASEcoord: 1..331
NoneNo IPR availablePANTHERPTHR43317THERMOSPERMINE SYNTHASE ACAULIS5coord: 1..331
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 110..218
e-value: 2.39393E-7
score: 46.6543
IPR030374Polyamine biosynthesis domainPROSITEPS51006PABS_2coord: 29..266
score: 98.153557
IPR001045Spermidine/spermine synthasesHAMAPMF_00198Spermidine_synthcoord: 30..311
score: 31.244999
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 29..310

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS006005.1MS006005.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003824 catalytic activity