MS005872 (gene) Bitter gourd (TR) v1

Overview
NameMS005872
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionjasmonic acid-amido synthetase JAR1-like
Locationscaffold254: 2136951 .. 2139381 (+)
RNA-Seq ExpressionMS005872
SyntenyMS005872
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGGATAAAATGGAAGCATTTGATGGGGAGAAAGTGATAGAGCAATTCGAGGAGATGACCAAGGATGCAGAAAGAGTTCAGAGGGAGACTCTTGAGAAGATTTTGGAGGAAAATGGGTCGGCTGAGTACTTGCAGAGCTTGGGACTTAATGGAAGAACTGATCCTCAGAGCTTCAAGGACTGTGTCCCCCTTGTTACTCACAATGATTTGGAGCCATATATTCAGAGAATCGCGGATGGCGCCACTTCCCCCATTCTCACCGGCAAACCGATCAAAACCATTTCATTGAGGTACTTTTTGCAAATTTGTGCTGAGGAAGTTTTGCTTTTGATTCCAAGTATTGGAGATTTTGTTGCTGTTCCTTTTCTGAGGAGATTCTTGTTTTGTTGGCTGTATGATGTAGCTCTGGTACTACTCAGGGGAGGCCCAAGTTGATTCCCTTCAACGATGAACTCTTGGAGACGACAATGCAAATATATCGCACTTCTTTTGCCTTCAGAAACAAGTAATGGAACTTCTTTTCTGTTCTTGTGTCTCCTGTTTGGACGTTGTTTGTAGTCTGGCCTCTGAATCATTTCTTGTCTGAAATGAACATGGTTAATGCAGTTTGTTGTCTTTTTGACTGTCGGTTGTCATCTTAATAGTATAAAAATGGGCTTGTCATGGCTGATGTGGGGAGAGTTTTTTTGTTCTACAGAGAATTTCCCATTAGGAATGGAAAGGCCTTGCAGTTCATCTACAGCAGCAAGCAGTTCAAAACCAAAGGGGGTCTGGCAGCTGGAACTGCAACAACAAATGTTTACCGCAGTGCGAAGTTTAAGTGCACGATGAAAGCGATCCAGTCGCAGTGCTGTAGCCCGGACGAAGTCATCTTTGGCCCCGACTTCCACCAGTCACTGTATTGCCATCTCTTGTGTGGGCTGATCTTCCGCGATGAAGTTGAGTTTGTGTTCTCCACATTTGCACACAGCATTGTCCATGCTTTCAGGACTTTTGAGCTAGTCTGGGAAGAACTCTGCAGCAACATTAGAGATGGTGTTCTATCTAGTTGGGTCACCGCCCCCACCATTCGGGCAGCTATGTCGAAATTGCTTAGGCCAAATCCTGAATTGGCAGATTTGATCTATCGAAAATGCGCAGGGTTGAGTAACTGGTATGGACTGATACCGGAGCTCTTTCCTAACGCAAAGTACATTTACGGGATCATGACCGGGTCGATGGAGCCTTATCTGAAGAAACTGAGGCACTACGCAGGTGATCTGCTGCTGATGAGTGCAGATTATGGTTCTTCAGAAGGATGGGTTGGAGCAAATGTTAACCCAATGTTGCCCCCTGAAATGGCCACCTTTGTGGTTCTTCCGAACATCGGATACTTCGAGTTCATCCCATTAAAGGAGAGTGCTGAAGAACAGAAGCAGAACGAAGATGGAGCTCTGGTTGCAGCAGAGAACAAGCCAGTGGGTCTGACTGAAGTCAAGGTTGGTGAAGAGTACGAAATTATTGTCACCAATGTCGCAGGTAATTCCTTTCAATTCATGACATTATGCGCTATAACCCTGTTTTGTTTCCTCCAAACCAAACATGAATGAATAGGATGACTGGGTGACAGTTGTGCTCCCAAAATATATTATTGACTCACCATCCAATTCATATGTTATGTTGGATTATTAAAATCTCTTGTTTGTTTCAATGCTCCCCATGTGACCTTGGGGGGGTCTGCAGGATCAATCTCTGCCTCTTGCTTTGTCTTCTTCTGTCCTACGTGTCGTCGAGACGTTGAATTGTCCACTTTAAACCTAAACCCGCTTGATAAAACCGAATTTATTTTCATTCGAACTGTACTCTAGAAGTCTCCACTGGCATTAATGTAGATATTAACTATGGACATTGCATTATAGGGCTGTACCGTTACAGACTGGGGGATGTGGTGAAGGTGATGGGGTTCCATAACGCGACCCCAGAGGTGAAATTCGTGTGCAGGAGGAACCTTCTGCTTAGCATCAACATCGACAAGAACACGGAGAAGGACCTGCAGGTGGCGGTCGAGGCAGCCGGGAGCCTGCTGGCGGAGGAGAAGCTGGAGGTGGTGGACTTCACGAGCCATGTCGACATGTCGAGAGAGCCAGGGCACTACGTGATATTCTGGGAGACGAGCGGAGAGGCGAAGGGGGAGGTCCTGGGAGAGTGCTGCAACTGCCTGGACCGGGCGTTCCTGGACGCAGGATACGTGAGCTCGAGGAAGGTGAATGCCATTGGAGCACTGGAGCTGAGGGTGGTCCGGAAGGGGACCTTTCACAAGATCATGGACCACTATTTGTCGCTAGGGGCCGCTGTGAGCCAATACAAAACACCCCGTTGTGTTATCCCCACCAACACAACTGTGTTGCAGATCTTGTGCGCCAATGTCGTTAACTCCTATTTCAGTACT

mRNA sequence

ATGTTGGATAAAATGGAAGCATTTGATGGGGAGAAAGTGATAGAGCAATTCGAGGAGATGACCAAGGATGCAGAAAGAGTTCAGAGGGAGACTCTTGAGAAGATTTTGGAGGAAAATGGGTCGGCTGAGTACTTGCAGAGCTTGGGACTTAATGGAAGAACTGATCCTCAGAGCTTCAAGGACTGTGTCCCCCTTGTTACTCACAATGATTTGGAGCCATATATTCAGAGAATCGCGGATGGCGCCACTTCCCCCATTCTCACCGGCAAACCGATCAAAACCATTTCATTGAGGTACTTTTTGCAAATTTGTGCTGAGGAAGTTTTGCTTTTGATTCCAAGTATTGGAGATTTTGTTGCTGTTCCTTTTCTGAGGAGATTCTTGTTTTTTTTTTTGTTCTACAGAGAATTTCCCATTAGGAATGGAAAGGCCTTGCAGTTCATCTACAGCAGCAAGCAGTTCAAAACCAAAGGGGGTCTGGCAGCTGGAACTGCAACAACAAATGTTTACCGCAGTGCGAAGTTTAAGTGCACGATGAAAGCGATCCAGTCGCAGTGCTGTAGCCCGGACGAAGTCATCTTTGGCCCCGACTTCCACCAGTCACTGTATTGCCATCTCTTGTGTGGGCTGATCTTCCGCGATGAAGTTGAGTTTGTGTTCTCCACATTTGCACACAGCATTGTCCATGCTTTCAGGACTTTTGAGCTAGTCTGGGAAGAACTCTGCAGCAACATTAGAGATGGTGTTCTATCTAGTTGGGTCACCGCCCCCACCATTCGGGCAGCTATGTCGAAATTGCTTAGGCCAAATCCTGAATTGGCAGATTTGATCTATCGAAAATGCGCAGGGTTGAGTAACTGGTATGGACTGATACCGGAGCTCTTTCCTAACGCAAAGTACATTTACGGGATCATGACCGGGTCGATGGAGCCTTATCTGAAGAAACTGAGGCACTACGCAGGTGATCTGCTGCTGATGAGTGCAGATTATGGTTCTTCAGAAGGATGGGTTGGAGCAAATGTTAACCCAATGTTGCCCCCTGAAATGGCCACCTTTGTGGTTCTTCCGAACATCGGATACTTCGAGTTCATCCCATTAAAGGAGAGTGCTGAAGAACAGAAGCAGAACGAAGATGGAGCTCTGGTTGCAGCAGAGAACAAGCCAGTGGGTCTGACTGAAGTCAAGGTTGGTGAAGAGTACGAAATTATTGTCACCAATGTCGCAGGGCTGTACCGTTACAGACTGGGGGATGTGGTGAAGGTGATGGGGTTCCATAACGCGACCCCAGAGGTGAAATTCGTGTGCAGGAGGAACCTTCTGCTTAGCATCAACATCGACAAGAACACGGAGAAGGACCTGCAGGTGGCGGTCGAGGCAGCCGGGAGCCTGCTGGCGGAGGAGAAGCTGGAGGTGGTGGACTTCACGAGCCATGTCGACATGTCGAGAGAGCCAGGGCACTACGTGATATTCTGGGAGACGAGCGGAGAGGCGAAGGGGGAGGTCCTGGGAGAGTGCTGCAACTGCCTGGACCGGGCGTTCCTGGACGCAGGATACGTGAGCTCGAGGAAGGTGAATGCCATTGGAGCACTGGAGCTGAGGGTGGTCCGGAAGGGGACCTTTCACAAGATCATGGACCACTATTTGTCGCTAGGGGCCGCTGTGAGCCAATACAAAACACCCCGTTGTGTTATCCCCACCAACACAACTGTGTTGCAGATCTTGTGCGCCAATGTCGTTAACTCCTATTTCAGTACT

Coding sequence (CDS)

ATGTTGGATAAAATGGAAGCATTTGATGGGGAGAAAGTGATAGAGCAATTCGAGGAGATGACCAAGGATGCAGAAAGAGTTCAGAGGGAGACTCTTGAGAAGATTTTGGAGGAAAATGGGTCGGCTGAGTACTTGCAGAGCTTGGGACTTAATGGAAGAACTGATCCTCAGAGCTTCAAGGACTGTGTCCCCCTTGTTACTCACAATGATTTGGAGCCATATATTCAGAGAATCGCGGATGGCGCCACTTCCCCCATTCTCACCGGCAAACCGATCAAAACCATTTCATTGAGGTACTTTTTGCAAATTTGTGCTGAGGAAGTTTTGCTTTTGATTCCAAGTATTGGAGATTTTGTTGCTGTTCCTTTTCTGAGGAGATTCTTGTTTTTTTTTTTGTTCTACAGAGAATTTCCCATTAGGAATGGAAAGGCCTTGCAGTTCATCTACAGCAGCAAGCAGTTCAAAACCAAAGGGGGTCTGGCAGCTGGAACTGCAACAACAAATGTTTACCGCAGTGCGAAGTTTAAGTGCACGATGAAAGCGATCCAGTCGCAGTGCTGTAGCCCGGACGAAGTCATCTTTGGCCCCGACTTCCACCAGTCACTGTATTGCCATCTCTTGTGTGGGCTGATCTTCCGCGATGAAGTTGAGTTTGTGTTCTCCACATTTGCACACAGCATTGTCCATGCTTTCAGGACTTTTGAGCTAGTCTGGGAAGAACTCTGCAGCAACATTAGAGATGGTGTTCTATCTAGTTGGGTCACCGCCCCCACCATTCGGGCAGCTATGTCGAAATTGCTTAGGCCAAATCCTGAATTGGCAGATTTGATCTATCGAAAATGCGCAGGGTTGAGTAACTGGTATGGACTGATACCGGAGCTCTTTCCTAACGCAAAGTACATTTACGGGATCATGACCGGGTCGATGGAGCCTTATCTGAAGAAACTGAGGCACTACGCAGGTGATCTGCTGCTGATGAGTGCAGATTATGGTTCTTCAGAAGGATGGGTTGGAGCAAATGTTAACCCAATGTTGCCCCCTGAAATGGCCACCTTTGTGGTTCTTCCGAACATCGGATACTTCGAGTTCATCCCATTAAAGGAGAGTGCTGAAGAACAGAAGCAGAACGAAGATGGAGCTCTGGTTGCAGCAGAGAACAAGCCAGTGGGTCTGACTGAAGTCAAGGTTGGTGAAGAGTACGAAATTATTGTCACCAATGTCGCAGGGCTGTACCGTTACAGACTGGGGGATGTGGTGAAGGTGATGGGGTTCCATAACGCGACCCCAGAGGTGAAATTCGTGTGCAGGAGGAACCTTCTGCTTAGCATCAACATCGACAAGAACACGGAGAAGGACCTGCAGGTGGCGGTCGAGGCAGCCGGGAGCCTGCTGGCGGAGGAGAAGCTGGAGGTGGTGGACTTCACGAGCCATGTCGACATGTCGAGAGAGCCAGGGCACTACGTGATATTCTGGGAGACGAGCGGAGAGGCGAAGGGGGAGGTCCTGGGAGAGTGCTGCAACTGCCTGGACCGGGCGTTCCTGGACGCAGGATACGTGAGCTCGAGGAAGGTGAATGCCATTGGAGCACTGGAGCTGAGGGTGGTCCGGAAGGGGACCTTTCACAAGATCATGGACCACTATTTGTCGCTAGGGGCCGCTGTGAGCCAATACAAAACACCCCGTTGTGTTATCCCCACCAACACAACTGTGTTGCAGATCTTGTGCGCCAATGTCGTTAACTCCTATTTCAGTACT

Protein sequence

MLDKMEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSFKDCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLRYFLQICAEEVLLLIPSIGDFVAVPFLRRFLFFFLFYREFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMKAIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEELCSNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYGIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFEFIPLKESAEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVKVMGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDMSREPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTFHKIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFST
Homology
BLAST of MS005872 vs. NCBI nr
Match: XP_022147427.1 (jasmonic acid-amido synthetase JAR1-like [Momordica charantia] >XP_022147428.1 jasmonic acid-amido synthetase JAR1-like [Momordica charantia])

HSP 1 Score: 1088.9 bits (2815), Expect = 0.0e+00
Identity = 550/585 (94.02%), Postives = 554/585 (94.70%), Query Frame = 0

Query: 1   MLDKMEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSFK 60
           MLDKMEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSFK
Sbjct: 1   MLDKMEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSFK 60

Query: 61  DCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLRYFLQICAEEVLLLIPSIGDFVA 120
           DCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISL        +    LIP   + + 
Sbjct: 61  DCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLS---SGTTQGRPKLIPFNDELLE 120

Query: 121 VPFLRRFLFFFLFYREFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMK 180
                    F    +EFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMK
Sbjct: 121 TTMQIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMK 180

Query: 181 AIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEE 240
           AIQS CCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEE
Sbjct: 181 AIQSLCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEE 240

Query: 241 LCSNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKY 300
           LCSNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKY
Sbjct: 241 LCSNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKY 300

Query: 301 IYGIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGY 360
           IYGIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGY
Sbjct: 301 IYGIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGY 360

Query: 361 FEFIPLKESAEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVK 420
           FEFIPLKESAEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVK
Sbjct: 361 FEFIPLKESAEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVK 420

Query: 421 VMGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDM 480
           VMGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDM
Sbjct: 421 VMGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDM 480

Query: 481 SREPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTF 540
           SREPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTF
Sbjct: 481 SREPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTF 540

Query: 541 HKIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFST 586
           HKIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFST
Sbjct: 541 HKIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFST 582

BLAST of MS005872 vs. NCBI nr
Match: XP_023539820.1 (jasmonic acid-amido synthetase JAR1 [Cucurbita pepo subsp. pepo] >XP_023539821.1 jasmonic acid-amido synthetase JAR1 [Cucurbita pepo subsp. pepo] >XP_023539822.1 jasmonic acid-amido synthetase JAR1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 990.7 bits (2560), Expect = 5.3e-285
Identity = 501/587 (85.35%), Postives = 535/587 (91.14%), Query Frame = 0

Query: 1   MLDK-MEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSF 60
           ML+K MEAFDGEKVIEQFEEMT+DAERVQ+ETL+KILEENGSAEYLQ+LGLNGRTDPQSF
Sbjct: 1   MLEKMMEAFDGEKVIEQFEEMTRDAERVQKETLKKILEENGSAEYLQNLGLNGRTDPQSF 60

Query: 61  KDCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLRYFLQICAEEVLLLIPSIGDFV 120
           KDCVPLV H+DLE YIQRIADGA+SPILTGKPIKTISL        +    LIP   + +
Sbjct: 61  KDCVPLVYHDDLESYIQRIADGASSPILTGKPIKTISLS---SGTTQGRPKLIPFNDELL 120

Query: 121 AVPFLRRFLFFFLFYREFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTM 180
                     F    +EFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFK TM
Sbjct: 121 ETTMQIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKSTM 180

Query: 181 KAIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWE 240
           K IQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEV+FVFSTFAHSIVHAFRTFELVWE
Sbjct: 181 KTIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVQFVFSTFAHSIVHAFRTFELVWE 240

Query: 241 ELCSNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAK 300
           ELC +IRDGVLSS VTA +IR AMSK+L+PNP+LADLIY+KCAGLSNWYGLIPELFPNA+
Sbjct: 241 ELCGDIRDGVLSSRVTALSIRTAMSKILKPNPDLADLIYQKCAGLSNWYGLIPELFPNAQ 300

Query: 301 YIYGIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIG 360
           YIYGIMTGSMEPYLKKLRHYAG L LMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIG
Sbjct: 301 YIYGIMTGSMEPYLKKLRHYAGHLPLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIG 360

Query: 361 YFEFIPLKESAEEQKQNED-GALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDV 420
           YFEFIPLKE++++Q+Q  D GA+VA ENKPVGLTEVKVGE YEIIVTNVAGLYRYRLGDV
Sbjct: 361 YFEFIPLKENSQDQEQEGDGGAIVAKENKPVGLTEVKVGEVYEIIVTNVAGLYRYRLGDV 420

Query: 421 VKVMGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHV 480
           VKVMGFHNATPE+KF+CR NLLLSINIDKNTEKDLQ+AVEAAG++LA EKLEVV+FTSHV
Sbjct: 421 VKVMGFHNATPELKFMCRSNLLLSINIDKNTEKDLQLAVEAAGNVLAAEKLEVVEFTSHV 480

Query: 481 DMSREPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKG 540
           D+SREPGHYVIFWE SGEAKGEVLGECCNCLDRAFLDAGY+SSRKVNAIGALELRVV KG
Sbjct: 481 DLSREPGHYVIFWEISGEAKGEVLGECCNCLDRAFLDAGYMSSRKVNAIGALELRVVGKG 540

Query: 541 TFHKIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFST 586
           TFHKIM+HYLSLGAAVSQYKTPRCV P NTTVLQILCANVVNSYFST
Sbjct: 541 TFHKIMNHYLSLGAAVSQYKTPRCVSPANTTVLQILCANVVNSYFST 584

BLAST of MS005872 vs. NCBI nr
Match: XP_022974649.1 (jasmonic acid-amido synthetase JAR1 [Cucurbita maxima] >XP_022974650.1 jasmonic acid-amido synthetase JAR1 [Cucurbita maxima] >XP_022974651.1 jasmonic acid-amido synthetase JAR1 [Cucurbita maxima])

HSP 1 Score: 989.2 bits (2556), Expect = 1.5e-284
Identity = 501/587 (85.35%), Postives = 533/587 (90.80%), Query Frame = 0

Query: 1   MLDKM-EAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSF 60
           ML+KM EAFDGEKVIEQFEEMT+DAERVQ+ETL+KILEENGSAEYLQ+LGLNGRTDPQSF
Sbjct: 1   MLEKMVEAFDGEKVIEQFEEMTRDAERVQKETLKKILEENGSAEYLQNLGLNGRTDPQSF 60

Query: 61  KDCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLRYFLQICAEEVLLLIPSIGDFV 120
           KDCVPLV H DLE YIQRIADGA+SPILTGKPIKTISL        +    LIP   + +
Sbjct: 61  KDCVPLVYHEDLESYIQRIADGASSPILTGKPIKTISLS---SGTTQGRPKLIPFNDELL 120

Query: 121 AVPFLRRFLFFFLFYREFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTM 180
                     F    +EFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFK TM
Sbjct: 121 ETTMQIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKSTM 180

Query: 181 KAIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWE 240
           K IQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEV+FVFSTFAHSIVHAFRTFELVWE
Sbjct: 181 KTIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVQFVFSTFAHSIVHAFRTFELVWE 240

Query: 241 ELCSNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAK 300
           ELC +IRDGVLSS VTA +IR AMSK+L+PNPELADLIY+KCAGLSNWYGLIPELFPNA+
Sbjct: 241 ELCGDIRDGVLSSRVTALSIRTAMSKILKPNPELADLIYQKCAGLSNWYGLIPELFPNAQ 300

Query: 301 YIYGIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIG 360
           YIYGIMTGSMEPYLKKLRHYAG L LMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIG
Sbjct: 301 YIYGIMTGSMEPYLKKLRHYAGHLPLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIG 360

Query: 361 YFEFIPLKESAEEQKQNED-GALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDV 420
           YFEFIPLKE++++Q+Q  D GA+VA ENKPVGLTEVKVGE YEIIVTNVAGLYRYRLGDV
Sbjct: 361 YFEFIPLKENSQDQEQEGDGGAIVAKENKPVGLTEVKVGEVYEIIVTNVAGLYRYRLGDV 420

Query: 421 VKVMGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHV 480
           VKVMGFHNATPE+KF+CR NLLLSINIDKNTEKDLQ+AVEAAG++LA EKLEVV+FTSHV
Sbjct: 421 VKVMGFHNATPELKFMCRSNLLLSINIDKNTEKDLQLAVEAAGNVLAAEKLEVVEFTSHV 480

Query: 481 DMSREPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKG 540
           D+SREPGHYVIFWE SGEAKGEVLGECCNCLDRAFLDAGY+SSRKVNAIGALELRVV KG
Sbjct: 481 DLSREPGHYVIFWEISGEAKGEVLGECCNCLDRAFLDAGYMSSRKVNAIGALELRVVGKG 540

Query: 541 TFHKIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFST 586
           TFHKIM+HYLSLGAAVSQYKTPRCV P NTTVLQILCANVV SYFST
Sbjct: 541 TFHKIMNHYLSLGAAVSQYKTPRCVSPANTTVLQILCANVVKSYFST 584

BLAST of MS005872 vs. NCBI nr
Match: KAG6597096.1 (Jasmonoyl--L-amino acid synthetase JAR6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 988.4 bits (2554), Expect = 2.6e-284
Identity = 500/587 (85.18%), Postives = 534/587 (90.97%), Query Frame = 0

Query: 1   MLDK-MEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSF 60
           ML+K MEAFDGEKVIEQFEEMT+DAERVQ+ETL+KILEENGSAEYLQ+LGLNGRTDPQSF
Sbjct: 29  MLEKMMEAFDGEKVIEQFEEMTRDAERVQKETLKKILEENGSAEYLQNLGLNGRTDPQSF 88

Query: 61  KDCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLRYFLQICAEEVLLLIPSIGDFV 120
           KDCVPLV H+DLE YIQRIADGA+SPILTGKPIKTISL        +    LIP   + +
Sbjct: 89  KDCVPLVYHDDLESYIQRIADGASSPILTGKPIKTISLS---SGTTQGRPKLIPFNDELL 148

Query: 121 AVPFLRRFLFFFLFYREFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTM 180
                     F    +EFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFK TM
Sbjct: 149 ETTMQIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKSTM 208

Query: 181 KAIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWE 240
           K IQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEV+FVFSTFAHSIVHAFRTFELVWE
Sbjct: 209 KTIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVQFVFSTFAHSIVHAFRTFELVWE 268

Query: 241 ELCSNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAK 300
           ELC +IRDGVLSS VTA +IR AMSK+L+PNPELADLIY+KCAGLSNWYGLIPELFPNA+
Sbjct: 269 ELCGDIRDGVLSSRVTALSIRTAMSKILKPNPELADLIYQKCAGLSNWYGLIPELFPNAQ 328

Query: 301 YIYGIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIG 360
           YIYGIMTGSMEPYLKKLRHYAG L LMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIG
Sbjct: 329 YIYGIMTGSMEPYLKKLRHYAGHLPLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIG 388

Query: 361 YFEFIPLKESAEEQKQNED-GALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDV 420
           YFEFIPLKE++++Q+Q  D GA+VA ENKPVGLTEVKVGE YEIIVTNVAGLYRYRLGD+
Sbjct: 389 YFEFIPLKENSQDQEQEGDGGAIVAKENKPVGLTEVKVGEVYEIIVTNVAGLYRYRLGDL 448

Query: 421 VKVMGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHV 480
           VKVMGFHNATPE+KF+CR NLLLSINIDKNTEKDLQ+AVEAAG++LA EKLEVV+FTSHV
Sbjct: 449 VKVMGFHNATPELKFMCRSNLLLSINIDKNTEKDLQLAVEAAGNVLAAEKLEVVEFTSHV 508

Query: 481 DMSREPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKG 540
           D+SREPGHYVIFWE SGEAKGEVLGECCNCLDRAFLDAGY+SSRKVNAIGALELRVV KG
Sbjct: 509 DLSREPGHYVIFWEISGEAKGEVLGECCNCLDRAFLDAGYMSSRKVNAIGALELRVVGKG 568

Query: 541 TFHKIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFST 586
           TFHKIM+HYLSLGAAVSQYKTPRCV P NTTVLQILCANVV SYFST
Sbjct: 569 TFHKIMNHYLSLGAAVSQYKTPRCVSPANTTVLQILCANVVKSYFST 612

BLAST of MS005872 vs. NCBI nr
Match: XP_022941396.1 (jasmonic acid-amido synthetase JAR1 [Cucurbita moschata] >XP_022941403.1 jasmonic acid-amido synthetase JAR1 [Cucurbita moschata] >XP_022941412.1 jasmonic acid-amido synthetase JAR1 [Cucurbita moschata])

HSP 1 Score: 988.4 bits (2554), Expect = 2.6e-284
Identity = 500/587 (85.18%), Postives = 534/587 (90.97%), Query Frame = 0

Query: 1   MLDK-MEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSF 60
           ML+K MEAFDGEKVIEQFEEMT+DAERVQ+ETL+KILEENGSAEYLQ+LGLNGRTDPQSF
Sbjct: 1   MLEKMMEAFDGEKVIEQFEEMTRDAERVQKETLKKILEENGSAEYLQNLGLNGRTDPQSF 60

Query: 61  KDCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLRYFLQICAEEVLLLIPSIGDFV 120
           KDCVPLV H+DLE YIQRIADGA+SPILTGKPIKTISL        +    LIP   + +
Sbjct: 61  KDCVPLVYHDDLESYIQRIADGASSPILTGKPIKTISLS---SGTTQGRPKLIPFNDELL 120

Query: 121 AVPFLRRFLFFFLFYREFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTM 180
                     F    +EFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFK TM
Sbjct: 121 ETTMQIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKSTM 180

Query: 181 KAIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWE 240
           K IQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEV+FVFSTFAHSIVHAFRTFELVWE
Sbjct: 181 KTIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVQFVFSTFAHSIVHAFRTFELVWE 240

Query: 241 ELCSNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAK 300
           ELC +IRDGVLSS VTA +IR AMSK+L+PNPELADLIY+KCAGLSNWYGLIPELFPNA+
Sbjct: 241 ELCGDIRDGVLSSRVTALSIRTAMSKILKPNPELADLIYQKCAGLSNWYGLIPELFPNAQ 300

Query: 301 YIYGIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIG 360
           YIYGIMTGSMEPYLKKLRHYAG L LMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIG
Sbjct: 301 YIYGIMTGSMEPYLKKLRHYAGHLPLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIG 360

Query: 361 YFEFIPLKESAEEQKQNED-GALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDV 420
           YFEFIPLKE++++Q+Q  D GA+VA ENKPVGLTEVKVGE YEIIVTNVAGLYRYRLGD+
Sbjct: 361 YFEFIPLKENSQDQEQEGDGGAIVAKENKPVGLTEVKVGEVYEIIVTNVAGLYRYRLGDL 420

Query: 421 VKVMGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHV 480
           VKVMGFHNATPE+KF+CR NLLLSINIDKNTEKDLQ+AVEAAG++LA EKLEVV+FTSHV
Sbjct: 421 VKVMGFHNATPELKFMCRSNLLLSINIDKNTEKDLQLAVEAAGNVLAAEKLEVVEFTSHV 480

Query: 481 DMSREPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKG 540
           D+SREPGHYVIFWE SGEAKGEVLGECCNCLDRAFLDAGY+SSRKVNAIGALELRVV KG
Sbjct: 481 DLSREPGHYVIFWEISGEAKGEVLGECCNCLDRAFLDAGYMSSRKVNAIGALELRVVGKG 540

Query: 541 TFHKIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFST 586
           TFHKIM+HYLSLGAAVSQYKTPRCV P NTTVLQILCANVV SYFST
Sbjct: 541 TFHKIMNHYLSLGAAVSQYKTPRCVSPANTTVLQILCANVVKSYFST 584

BLAST of MS005872 vs. ExPASy Swiss-Prot
Match: A0A1J6KGJ9 (Jasmonoyl--L-amino acid synthetase JAR4 OS=Nicotiana attenuata OX=49451 GN=JAR4 PE=1 SV=1)

HSP 1 Score: 847.8 bits (2189), Expect = 7.2e-245
Identity = 419/585 (71.62%), Postives = 486/585 (83.08%), Query Frame = 0

Query: 1   MLDKMEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSFK 60
           +++K E FD E+VIE+FE +TKDA ++Q ETL+KILEENG  EYLQ  GLNG+TD  SFK
Sbjct: 4   VVEKTEKFDPEEVIEEFEVLTKDAGKIQEETLQKILEENGGTEYLQQWGLNGKTDSLSFK 63

Query: 61  DCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLRYFLQICAEEVLLLIPSIGDFVA 120
           +C+P+VTH DLEPYI RIADG  SPILTGKPI TISL         + +     + +   
Sbjct: 64  NCIPIVTHKDLEPYIHRIADGDLSPILTGKPITTISLSSGTTQGKPKFVPFNEELMESTM 123

Query: 121 VPFLRRFLFFFLFYREFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMK 180
             F   F+F     REFP+ NGKALQFIY SKQFKTKGGLAAGTATTNVYR+A+FK TMK
Sbjct: 124 QIFKTSFVF---RNREFPVVNGKALQFIYGSKQFKTKGGLAAGTATTNVYRNAQFKKTMK 183

Query: 181 AIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEE 240
           A+Q+ CCSPDEVIFGPDF QSLYCHLLCGLIFRDEV+ V STFAHSIVHAFR FE +W+E
Sbjct: 184 AMQTPCCSPDEVIFGPDFQQSLYCHLLCGLIFRDEVQVVSSTFAHSIVHAFRNFEQIWQE 243

Query: 241 LCSNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKY 300
           L +NIR+GVLSS V  P++RAAMSKLL+P+PELAD I+ KC+ LSNWYGLIPELFPN +Y
Sbjct: 244 LVTNIREGVLSSRVIVPSMRAAMSKLLKPDPELADTIFNKCSRLSNWYGLIPELFPNTRY 303

Query: 301 IYGIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGY 360
           IYGIMTGSMEPYLKKLRHYAGDL L+SADYGSSEGW+GANVNP LPPE+ T+ VLPNIGY
Sbjct: 304 IYGIMTGSMEPYLKKLRHYAGDLPLLSADYGSSEGWIGANVNPELPPELVTYAVLPNIGY 363

Query: 361 FEFIPLKESAEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVK 420
           FEFIPL E       N DG     E  PVGLTEVK+GEEYEI+VTN AGLYRYRLGDVVK
Sbjct: 364 FEFIPLME-------NLDG----LEPMPVGLTEVKLGEEYEIVVTNFAGLYRYRLGDVVK 423

Query: 421 VMGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDM 480
           + GFHN TPE++F+CRRNLLLSINIDKNTEKDLQ+AVEAA  +L++EKLEVVDFTSHV++
Sbjct: 424 IKGFHNGTPELQFICRRNLLLSINIDKNTEKDLQLAVEAAAKILSDEKLEVVDFTSHVNV 483

Query: 481 SREPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTF 540
           S +PGHYVIFWE +GEA  E+L ECCNCLD++F+DAGYV SRKV+AIGALELR+V++GTF
Sbjct: 484 SADPGHYVIFWELNGEASEEILKECCNCLDKSFVDAGYVGSRKVHAIGALELRIVKRGTF 543

Query: 541 HKIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFST 586
           HKI+DH++ LGAAVSQ+KTPRCV PTN +VLQIL +NVV SYFST
Sbjct: 544 HKILDHFVGLGAAVSQFKTPRCVGPTNLSVLQILSSNVVESYFST 574

BLAST of MS005872 vs. ExPASy Swiss-Prot
Match: A0A314KSQ4 (Jasmonoyl--L-amino acid synthetase JAR6 OS=Nicotiana attenuata OX=49451 GN=JAR6 PE=1 SV=2)

HSP 1 Score: 841.3 bits (2172), Expect = 6.8e-243
Identity = 422/587 (71.89%), Postives = 487/587 (82.96%), Query Frame = 0

Query: 1   MLDKME-AFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSF 60
           +++K+E  FD EKVIE+FE++TKDA ++Q ETL+KILE+NG  EYLQ  GLNGRTDPQ+F
Sbjct: 4   LVEKIEKKFDQEKVIEEFEDLTKDAGKIQEETLKKILEQNGGTEYLQLWGLNGRTDPQTF 63

Query: 61  KDCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLRYFLQICAEEVLLLIPSIGDFV 120
           K+CVP+VTHNDLEPYIQRIADG  SPILTGKPI+TISL        +     +P   D +
Sbjct: 64  KNCVPIVTHNDLEPYIQRIADGDLSPILTGKPIETISLS---SGTTQGKPKFVP-FNDEL 123

Query: 121 AVPFLRRFLFFFLF-YREFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCT 180
               ++ F   F F  REFPI NGKALQFIYSSKQFKTKGGLAAGTATTNVYR+A+FK T
Sbjct: 124 MESTMQIFKTSFAFRNREFPIGNGKALQFIYSSKQFKTKGGLAAGTATTNVYRNAQFKKT 183

Query: 181 MKAIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVW 240
           MKA+ + CCSPDEVIFGPDFHQSLYCHLLCGLIF DEV+ V STFAHSIVHAFRTFE VW
Sbjct: 184 MKAMCTPCCSPDEVIFGPDFHQSLYCHLLCGLIFHDEVQVVSSTFAHSIVHAFRTFEQVW 243

Query: 241 EELCSNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNA 300
           E L  +IR+GVLSS VT P+IR AMSKLL+P+PELAD IY KC+ LSNWYGLIP+LFPN 
Sbjct: 244 EALVVDIREGVLSSRVTVPSIRLAMSKLLKPDPELADTIYNKCSRLSNWYGLIPDLFPNT 303

Query: 301 KYIYGIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNI 360
           +YIYGIMTGSMEPYLKKLRHYAG+L L+SADYGSSEGWVG NVNP LPPE+ T+ VLPNI
Sbjct: 304 RYIYGIMTGSMEPYLKKLRHYAGELPLLSADYGSSEGWVGVNVNPKLPPELVTYAVLPNI 363

Query: 361 GYFEFIPLKESAEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDV 420
           GYFEFIPL  +    +Q          N PVGLTEVK+GEEYE++ TN AGLYRYRLGDV
Sbjct: 364 GYFEFIPLGGNLNGIEQ---------ANSPVGLTEVKLGEEYEVVFTNFAGLYRYRLGDV 423

Query: 421 VKVMGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHV 480
           VKV GFHN TPE++FVCR NLLLSINIDKNTEKDLQ+AVEAA   L +EKLEVVDFTSHV
Sbjct: 424 VKVKGFHNGTPELQFVCRSNLLLSINIDKNTEKDLQLAVEAAAKRLVDEKLEVVDFTSHV 483

Query: 481 DMSREPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKG 540
           ++S +PGHYVIFWE SGEA  E+L +CCNCLDR+F+DAGYVSSRKVNAIGALELR+V++G
Sbjct: 484 NVSADPGHYVIFWELSGEATDEMLQDCCNCLDRSFIDAGYVSSRKVNAIGALELRIVKRG 543

Query: 541 TFHKIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFST 586
           TFHKI+DH++ LG AVSQ+KTPRCV P N+++LQIL +NVV +Y ST
Sbjct: 544 TFHKILDHFVGLGGAVSQFKTPRCVGPKNSSLLQILSSNVVETYVST 577

BLAST of MS005872 vs. ExPASy Swiss-Prot
Match: Q6I581 (Jasmonoyl--L-amino acid synthetase GH3.5 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 3.4e-218
Identity = 370/576 (64.24%), Postives = 456/576 (79.17%), Query Frame = 0

Query: 11  EKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSFKDCVPLVTHND 70
           E+ I +FE +T+DA RVQ++TL+KILE N SAEYLQ+ GL GRTD +S+K C+PL  HND
Sbjct: 7   EETINEFEMLTRDAARVQKDTLKKILEINASAEYLQNFGLGGRTDAESYKSCIPLCVHND 66

Query: 71  LEPYIQRIADGATSPILTGKPIKTISLRYFLQICAEEVLLLIPSIGDFVAVPFLRRFLFF 130
           +EPYIQRI DG TSP++TG+PI  +SL         + +       D +    L+ +   
Sbjct: 67  IEPYIQRIVDGDTSPVVTGEPITNLSLSSGTTHGKPKFI----PFNDELLETTLQIYRTS 126

Query: 131 FLF-YREFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMKAIQSQCCSP 190
           + F  RE+PI  GKALQF+Y SKQ  TKGG+ A TATTN+YR  ++K  MK IQSQCCSP
Sbjct: 127 YAFRNREYPIGQGKALQFVYGSKQVITKGGILATTATTNLYRRQRYKEGMKDIQSQCCSP 186

Query: 191 DEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEELCSNIRDGV 250
           DEVIFGPDFHQSLYCHLLCGLI+ +EV  VFSTFAHS+VHAF+TFE VWE+LC++IRDGV
Sbjct: 187 DEVIFGPDFHQSLYCHLLCGLIYSEEVHSVFSTFAHSLVHAFQTFEEVWEDLCTDIRDGV 246

Query: 251 LSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYGIMTGSM 310
           LS  VTAP+IR A+SK+L+PNPELAD IY+KC GLSNWYG+IP L+PNAKY+YGIMTGSM
Sbjct: 247 LSKKVTAPSIREAVSKILKPNPELADSIYKKCIGLSNWYGVIPALWPNAKYVYGIMTGSM 306

Query: 311 EPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFEFIPLKES 370
           EPYLKKLRHYAG+L L+SADYG+SEGWVG+N++P +PPE  T+ VLP +GYFEFIPL++ 
Sbjct: 307 EPYLKKLRHYAGNLPLISADYGASEGWVGSNIDPTVPPEQVTYAVLPQVGYFEFIPLEKP 366

Query: 371 AEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVKVMGFHNATP 430
             E+ +N   ++   E+ PVGLTEV+VG+ YE+++TN AGLYRYRLGDVVK+  FHN+TP
Sbjct: 367 IGEETEN-SASIHYIESDPVGLTEVEVGKIYEVVITNFAGLYRYRLGDVVKIARFHNSTP 426

Query: 431 EVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDMSREPGHYVI 490
           E++F+CRR+L+LSINIDKNTEKDLQ+AVE A   L  EKLEV+DFTS V+ S +PG YVI
Sbjct: 427 ELQFICRRSLVLSINIDKNTEKDLQLAVEEASKFLEGEKLEVMDFTSFVERSSDPGRYVI 486

Query: 491 FWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTFHKIMDHYLS 550
           FWE SG+A  EVL  C N LD AF+DAGY  SRK+  IG LELR++RKGTF +I+DH+LS
Sbjct: 487 FWELSGDASDEVLSSCANALDLAFIDAGYTGSRKIKTIGPLELRILRKGTFKEILDHFLS 546

Query: 551 LGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFST 586
           LG AVSQ+KTPR V P+N+ VLQIL  NV  SYFST
Sbjct: 547 LGGAVSQFKTPRFVNPSNSKVLQILSRNVTQSYFST 577

BLAST of MS005872 vs. ExPASy Swiss-Prot
Match: Q9SKE2 (Jasmonoyl--L-amino acid synthetase JAR1 OS=Arabidopsis thaliana OX=3702 GN=JAR1 PE=1 SV=2)

HSP 1 Score: 745.7 bits (1924), Expect = 3.9e-214
Identity = 366/588 (62.24%), Postives = 457/588 (77.72%), Query Frame = 0

Query: 1   MLDKMEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGR-TDP-QS 60
           ML+K+E FD  +VI++F+EMT++A +VQ++TL++IL +N SA YLQ+ GLNG  TDP ++
Sbjct: 1   MLEKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATDPEEA 60

Query: 61  FKDCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLRYFLQICAEEVLLLIPSIGDF 120
           FK  VPLVT  +LEPYI+R+ DG TSPILTG P+  ISL         + +     + + 
Sbjct: 61  FKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMEN 120

Query: 121 VAVPFLRRFLFFFLFYREFPI-RNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKC 180
               F   F F     R+FPI  NGKALQFI+SSKQ+ + GG+  GTATTNVYR+  FK 
Sbjct: 121 TLQLFRTAFAF---RNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKA 180

Query: 181 TMKAIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELV 240
            MK+I S  CSPDEVIF PD HQ+LYCHLL G++FRD+V++VF+ FAH +VHAFRTFE V
Sbjct: 181 GMKSITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQV 240

Query: 241 WEELCSNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPN 300
           WEE+ ++I+DGVLS+ +T P++R AMSKLL PNPELA+ I  KC  LSNWYGLIP LFPN
Sbjct: 241 WEEIVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSLSNWYGLIPALFPN 300

Query: 301 AKYIYGIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPN 360
           AKY+YGIMTGSMEPY+ KLRHYAGDL L+S DYGSSEGW+ ANV P L PE ATF V+PN
Sbjct: 301 AKYVYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPN 360

Query: 361 IGYFEFIPLKESAEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGD 420
           +GYFEF+P+ E+ E             E KPVGLT+VK+GEEYE+++TN AGLYRYRLGD
Sbjct: 361 LGYFEFLPVSETGE------------GEEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGD 420

Query: 421 VVKVMGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSH 480
           VVKV+GF+N TP++KF+CRRNL+LSINIDKNTE+DLQ++VE+A   L+EEK+EV+DF+S+
Sbjct: 421 VVKVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEKIEVIDFSSY 480

Query: 481 VDMSREPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRK 540
           +D+S +PGHY IFWE SGE   +VL +CCNCLDRAF+DAGYVSSRK   IGALELRVV K
Sbjct: 481 IDVSTDPGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAK 540

Query: 541 GTFHKIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFST 586
           GTF KI +H+L LG++  Q+K PRCV P+N  VLQILC NVV+SYFST
Sbjct: 541 GTFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFST 573

BLAST of MS005872 vs. ExPASy Swiss-Prot
Match: Q53P49 (Probable indole-3-acetic acid-amido synthetase GH3.12 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.12 PE=2 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 6.9e-163
Identity = 303/600 (50.50%), Postives = 399/600 (66.50%), Query Frame = 0

Query: 9   DGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSFKDCVPLVTH 68
           + E+++  FE  T+DA  VQRETL +IL EN   EYL+ LGL G TD  SF+  VP+VTH
Sbjct: 13  EDEEIMAWFERTTRDAADVQRETLRRILAENAGVEYLRELGLAGLTDAGSFRARVPVVTH 72

Query: 69  NDLEPYIQRIADGATSPILTGKPIKTISLRYFLQICAEEVLLLIPSIGDFVAVPFLRRFL 128
            DL+PYIQR+ADG  SP+LT KP+  ISL         + LL      D +    +R F 
Sbjct: 73  ADLDPYIQRVADGDASPVLTAKPVTAISLSSGTTQGKRKRLL----FNDDLLRSSIRFFH 132

Query: 129 FFFLF-YREFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMKA---IQS 188
             + F  R FP+ +G+ LQF+Y S+   TKGGL A T  TN+ RS +F  +M A    + 
Sbjct: 133 ASYAFTNRAFPVEDGRVLQFMYGSRHETTKGGLTATTVMTNLLRSEEFTASMAARSRPRL 192

Query: 189 QCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEELCSN 248
             CSP EV+F PDF +SLYCHLLCGL+   EV  V ++FAHSIV A +  E VW ELC++
Sbjct: 193 PSCSPSEVVFSPDFDESLYCHLLCGLLLAGEVRAVSASFAHSIVVALQALERVWRELCAD 252

Query: 249 IRDGVLS-SWVTAPTIRAAMSKLL-RPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIY 308
           IR G  S + VT P +R A++ +L  PNP LAD + R+CA L +W G+IP L+PNA+Y+ 
Sbjct: 253 IRRGAASPARVTTPAVRRAVAPILAAPNPALADALERRCAALGDWSGVIPALWPNARYVQ 312

Query: 309 GIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFE 368
             MTGSME Y+KKLRHYAG + L+S +Y SSEG +G N     PPE   F VLP+  YFE
Sbjct: 313 ATMTGSMEHYVKKLRHYAGGVPLVSGNYASSEGVIGINAEQHAPPESVVFTVLPDAAYFE 372

Query: 369 FIPLKESAEEQKQNEDGALVAAEN--------KPVGLTEVKVGEEYEIIVTNVAGLYRYR 428
           FIPLK    +   ++D    A  +         PVGLT+V VGE YE+++T   GLYRYR
Sbjct: 373 FIPLKPPCTDAAADDDNPAAAGSSCYVDADDANPVGLTDVVVGEHYEVVMTTFTGLYRYR 432

Query: 429 LGDVVKVMGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLA-----EEKL 488
           LGDVVKV GFH+ATP+++FVCRR+L+LSIN+DKN+E DLQ+AV++A  +LA      ++L
Sbjct: 433 LGDVVKVAGFHHATPKLRFVCRRSLILSINVDKNSEHDLQLAVDSAAKILAGDGENHKQL 492

Query: 489 EVVDFTSHVDMSREPGHYVIFWETSG---EAKGEVLGECCNCLDRAF-LDAGYVSSRKVN 548
           E+ D+TSH D S +PGHYV+FWE +G   E  G VL  CC+ +DRAF  DAGY  SRK  
Sbjct: 493 EIADYTSHADTSSDPGHYVVFWELNGGGEEDGGGVLQRCCDEMDRAFGADAGYAQSRKTC 552

Query: 549 AIGALELRVVRKGTFHKIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFST 586
           AIGALELRV+R+G F +++ HY++ G++  Q+K PRCV P+N  VL++L  N +N +FST
Sbjct: 553 AIGALELRVLRRGAFQEVLRHYVAGGSSAGQFKMPRCVAPSNAGVLRVLKDNTINIFFST 608

BLAST of MS005872 vs. ExPASy TrEMBL
Match: A0A6J1D2B9 (jasmonic acid-amido synthetase JAR1-like OS=Momordica charantia OX=3673 GN=LOC111016355 PE=3 SV=1)

HSP 1 Score: 1088.9 bits (2815), Expect = 0.0e+00
Identity = 550/585 (94.02%), Postives = 554/585 (94.70%), Query Frame = 0

Query: 1   MLDKMEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSFK 60
           MLDKMEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSFK
Sbjct: 1   MLDKMEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSFK 60

Query: 61  DCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLRYFLQICAEEVLLLIPSIGDFVA 120
           DCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISL        +    LIP   + + 
Sbjct: 61  DCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLS---SGTTQGRPKLIPFNDELLE 120

Query: 121 VPFLRRFLFFFLFYREFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMK 180
                    F    +EFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMK
Sbjct: 121 TTMQIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMK 180

Query: 181 AIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEE 240
           AIQS CCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEE
Sbjct: 181 AIQSLCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEE 240

Query: 241 LCSNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKY 300
           LCSNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKY
Sbjct: 241 LCSNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKY 300

Query: 301 IYGIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGY 360
           IYGIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGY
Sbjct: 301 IYGIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGY 360

Query: 361 FEFIPLKESAEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVK 420
           FEFIPLKESAEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVK
Sbjct: 361 FEFIPLKESAEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVK 420

Query: 421 VMGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDM 480
           VMGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDM
Sbjct: 421 VMGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDM 480

Query: 481 SREPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTF 540
           SREPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTF
Sbjct: 481 SREPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTF 540

Query: 541 HKIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFST 586
           HKIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFST
Sbjct: 541 HKIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFST 582

BLAST of MS005872 vs. ExPASy TrEMBL
Match: A0A6J1II84 (jasmonic acid-amido synthetase JAR1 OS=Cucurbita maxima OX=3661 GN=LOC111473333 PE=3 SV=1)

HSP 1 Score: 989.2 bits (2556), Expect = 7.4e-285
Identity = 501/587 (85.35%), Postives = 533/587 (90.80%), Query Frame = 0

Query: 1   MLDKM-EAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSF 60
           ML+KM EAFDGEKVIEQFEEMT+DAERVQ+ETL+KILEENGSAEYLQ+LGLNGRTDPQSF
Sbjct: 1   MLEKMVEAFDGEKVIEQFEEMTRDAERVQKETLKKILEENGSAEYLQNLGLNGRTDPQSF 60

Query: 61  KDCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLRYFLQICAEEVLLLIPSIGDFV 120
           KDCVPLV H DLE YIQRIADGA+SPILTGKPIKTISL        +    LIP   + +
Sbjct: 61  KDCVPLVYHEDLESYIQRIADGASSPILTGKPIKTISLS---SGTTQGRPKLIPFNDELL 120

Query: 121 AVPFLRRFLFFFLFYREFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTM 180
                     F    +EFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFK TM
Sbjct: 121 ETTMQIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKSTM 180

Query: 181 KAIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWE 240
           K IQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEV+FVFSTFAHSIVHAFRTFELVWE
Sbjct: 181 KTIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVQFVFSTFAHSIVHAFRTFELVWE 240

Query: 241 ELCSNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAK 300
           ELC +IRDGVLSS VTA +IR AMSK+L+PNPELADLIY+KCAGLSNWYGLIPELFPNA+
Sbjct: 241 ELCGDIRDGVLSSRVTALSIRTAMSKILKPNPELADLIYQKCAGLSNWYGLIPELFPNAQ 300

Query: 301 YIYGIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIG 360
           YIYGIMTGSMEPYLKKLRHYAG L LMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIG
Sbjct: 301 YIYGIMTGSMEPYLKKLRHYAGHLPLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIG 360

Query: 361 YFEFIPLKESAEEQKQNED-GALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDV 420
           YFEFIPLKE++++Q+Q  D GA+VA ENKPVGLTEVKVGE YEIIVTNVAGLYRYRLGDV
Sbjct: 361 YFEFIPLKENSQDQEQEGDGGAIVAKENKPVGLTEVKVGEVYEIIVTNVAGLYRYRLGDV 420

Query: 421 VKVMGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHV 480
           VKVMGFHNATPE+KF+CR NLLLSINIDKNTEKDLQ+AVEAAG++LA EKLEVV+FTSHV
Sbjct: 421 VKVMGFHNATPELKFMCRSNLLLSINIDKNTEKDLQLAVEAAGNVLAAEKLEVVEFTSHV 480

Query: 481 DMSREPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKG 540
           D+SREPGHYVIFWE SGEAKGEVLGECCNCLDRAFLDAGY+SSRKVNAIGALELRVV KG
Sbjct: 481 DLSREPGHYVIFWEISGEAKGEVLGECCNCLDRAFLDAGYMSSRKVNAIGALELRVVGKG 540

Query: 541 TFHKIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFST 586
           TFHKIM+HYLSLGAAVSQYKTPRCV P NTTVLQILCANVV SYFST
Sbjct: 541 TFHKIMNHYLSLGAAVSQYKTPRCVSPANTTVLQILCANVVKSYFST 584

BLAST of MS005872 vs. ExPASy TrEMBL
Match: A0A6J1FL00 (jasmonic acid-amido synthetase JAR1 OS=Cucurbita moschata OX=3662 GN=LOC111446696 PE=3 SV=1)

HSP 1 Score: 988.4 bits (2554), Expect = 1.3e-284
Identity = 500/587 (85.18%), Postives = 534/587 (90.97%), Query Frame = 0

Query: 1   MLDK-MEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSF 60
           ML+K MEAFDGEKVIEQFEEMT+DAERVQ+ETL+KILEENGSAEYLQ+LGLNGRTDPQSF
Sbjct: 1   MLEKMMEAFDGEKVIEQFEEMTRDAERVQKETLKKILEENGSAEYLQNLGLNGRTDPQSF 60

Query: 61  KDCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLRYFLQICAEEVLLLIPSIGDFV 120
           KDCVPLV H+DLE YIQRIADGA+SPILTGKPIKTISL        +    LIP   + +
Sbjct: 61  KDCVPLVYHDDLESYIQRIADGASSPILTGKPIKTISLS---SGTTQGRPKLIPFNDELL 120

Query: 121 AVPFLRRFLFFFLFYREFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTM 180
                     F    +EFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFK TM
Sbjct: 121 ETTMQIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKSTM 180

Query: 181 KAIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWE 240
           K IQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEV+FVFSTFAHSIVHAFRTFELVWE
Sbjct: 181 KTIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVQFVFSTFAHSIVHAFRTFELVWE 240

Query: 241 ELCSNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAK 300
           ELC +IRDGVLSS VTA +IR AMSK+L+PNPELADLIY+KCAGLSNWYGLIPELFPNA+
Sbjct: 241 ELCGDIRDGVLSSRVTALSIRTAMSKILKPNPELADLIYQKCAGLSNWYGLIPELFPNAQ 300

Query: 301 YIYGIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIG 360
           YIYGIMTGSMEPYLKKLRHYAG L LMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIG
Sbjct: 301 YIYGIMTGSMEPYLKKLRHYAGHLPLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIG 360

Query: 361 YFEFIPLKESAEEQKQNED-GALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDV 420
           YFEFIPLKE++++Q+Q  D GA+VA ENKPVGLTEVKVGE YEIIVTNVAGLYRYRLGD+
Sbjct: 361 YFEFIPLKENSQDQEQEGDGGAIVAKENKPVGLTEVKVGEVYEIIVTNVAGLYRYRLGDL 420

Query: 421 VKVMGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHV 480
           VKVMGFHNATPE+KF+CR NLLLSINIDKNTEKDLQ+AVEAAG++LA EKLEVV+FTSHV
Sbjct: 421 VKVMGFHNATPELKFMCRSNLLLSINIDKNTEKDLQLAVEAAGNVLAAEKLEVVEFTSHV 480

Query: 481 DMSREPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKG 540
           D+SREPGHYVIFWE SGEAKGEVLGECCNCLDRAFLDAGY+SSRKVNAIGALELRVV KG
Sbjct: 481 DLSREPGHYVIFWEISGEAKGEVLGECCNCLDRAFLDAGYMSSRKVNAIGALELRVVGKG 540

Query: 541 TFHKIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFST 586
           TFHKIM+HYLSLGAAVSQYKTPRCV P NTTVLQILCANVV SYFST
Sbjct: 541 TFHKIMNHYLSLGAAVSQYKTPRCVSPANTTVLQILCANVVKSYFST 584

BLAST of MS005872 vs. ExPASy TrEMBL
Match: A0A5A7U0I3 (Jasmonic acid-amido synthetase JAR1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G001290 PE=3 SV=1)

HSP 1 Score: 980.7 bits (2534), Expect = 2.6e-282
Identity = 498/618 (80.58%), Postives = 530/618 (85.76%), Query Frame = 0

Query: 1   MLDKMEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSFK 60
           ML+KMEAFDGEKVIEQFEEMT+DAERVQRETL+KILEENGSAEYLQ+LGLNGRTDPQSFK
Sbjct: 1   MLEKMEAFDGEKVIEQFEEMTRDAERVQRETLKKILEENGSAEYLQNLGLNGRTDPQSFK 60

Query: 61  DCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISL----------------------- 120
           DCVPLV+H+DLE YIQRIADG +SPILTGKPIKTISL                       
Sbjct: 61  DCVPLVSHDDLESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDELLETTM 120

Query: 121 ----------RYFLQICAEEVLLLIPSIGDFVAVPFLRRFLFFFLFYREFPIRNGKALQF 180
                        L +C  +  +L      FV    +       LFYRE P+  GKALQF
Sbjct: 121 QIYRTSFAFRNNLLSLCCRDSRVLNTVKLVFVMADGVES----ILFYREVPLGKGKALQF 180

Query: 181 IYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMKAIQSQCCSPDEVIFGPDFHQSLYCHLL 240
           IYSSKQFKT GGLAAGTATTNVYRSA+FK TM+AIQSQCCSPDEVIFGPDFHQSLYCHLL
Sbjct: 181 IYSSKQFKTNGGLAAGTATTNVYRSAQFKSTMRAIQSQCCSPDEVIFGPDFHQSLYCHLL 240

Query: 241 CGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEELCSNIRDGVLSSWVTAPTIRAAMSKLL 300
           CGLIFRDEVEFVFSTFAHSIVH+FRTFE VWEELCSNIRDGVLSSWVTAP+IRAAMSKLL
Sbjct: 241 CGLIFRDEVEFVFSTFAHSIVHSFRTFEQVWEELCSNIRDGVLSSWVTAPSIRAAMSKLL 300

Query: 301 RPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYGIMTGSMEPYLKKLRHYAGDLLLMS 360
           +PNPELADLIYRKC GLSNWYGLIPELFPNAKYIYGIMTGSMEPYLKKLRHYAG L LMS
Sbjct: 301 KPNPELADLIYRKCEGLSNWYGLIPELFPNAKYIYGIMTGSMEPYLKKLRHYAGHLPLMS 360

Query: 361 ADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFEFIPLKESAEEQKQNEDGALVAAENK 420
           ADYGSSEGWVGANVNPMLPPEMATF VLPNIGYFEFIPLKE+A+ Q Q          NK
Sbjct: 361 ADYGSSEGWVGANVNPMLPPEMATFAVLPNIGYFEFIPLKENAQGQHQ---------RNK 420

Query: 421 PVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVKVMGFHNATPEVKFVCRRNLLLSINIDK 480
           P+GLTEVK+GEEYEIIVTNVAGLYRYRLGD VKVMGFHN+TPE+KF+CRRNLLLSINIDK
Sbjct: 421 PIGLTEVKIGEEYEIIVTNVAGLYRYRLGDAVKVMGFHNSTPELKFICRRNLLLSINIDK 480

Query: 481 NTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDMSREPGHYVIFWETSGEAKGEVLGECCN 540
            TEKDLQ+AVEAAG++LA EKLEVVDFTS+VD+SREPGHYVIFWE SGEAKGEVLGEC N
Sbjct: 481 ITEKDLQLAVEAAGNVLAAEKLEVVDFTSYVDVSREPGHYVIFWEISGEAKGEVLGECSN 540

Query: 541 CLDRAFLDAGYVSSRKVNAIGALELRVVRKGTFHKIMDHYLSLGAAVSQYKTPRCVIPTN 586
           CLDRAFLDAGY+SSRKVNAIGALELRVVRKGTFHKIMDH+LSLGAAVSQYKTPRCVIPTN
Sbjct: 541 CLDRAFLDAGYMSSRKVNAIGALELRVVRKGTFHKIMDHHLSLGAAVSQYKTPRCVIPTN 600

BLAST of MS005872 vs. ExPASy TrEMBL
Match: A0A1S4DTP6 (jasmonic acid-amido synthetase JAR1 OS=Cucumis melo OX=3656 GN=LOC103483208 PE=3 SV=1)

HSP 1 Score: 979.5 bits (2531), Expect = 5.9e-282
Identity = 493/585 (84.27%), Postives = 525/585 (89.74%), Query Frame = 0

Query: 1   MLDKMEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSFK 60
           ML+KMEAFDGEKVIEQFEEMT+DAERVQRETL+KILEENGSAEYLQ+LGLNGRTDPQSFK
Sbjct: 1   MLEKMEAFDGEKVIEQFEEMTRDAERVQRETLKKILEENGSAEYLQNLGLNGRTDPQSFK 60

Query: 61  DCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLRYFLQICAEEVLLLIPSIGDFVA 120
           DCVPLV+H+DLE YIQRIADG +SPILTGKPIKTISL        +    LIP   + + 
Sbjct: 61  DCVPLVSHDDLESYIQRIADGDSSPILTGKPIKTISLS---SGTTKGRPKLIPFNDELLE 120

Query: 121 VPFLRRFLFFFLFYREFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMK 180
                    F    +E P+  GKALQFIYSSKQFKT GGLAAGTATTNVYRSA+FK TM+
Sbjct: 121 TTMQIYRTSFAFRNKEVPLGKGKALQFIYSSKQFKTNGGLAAGTATTNVYRSAQFKSTMR 180

Query: 181 AIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEE 240
           AIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVH+FRTFE VWEE
Sbjct: 181 AIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHSFRTFEQVWEE 240

Query: 241 LCSNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKY 300
           LCSNIRDGVLSSWVTAP+IRAAMSKLL+PNPELADLIYRKC GLSNWYGLIPELFPNAKY
Sbjct: 241 LCSNIRDGVLSSWVTAPSIRAAMSKLLKPNPELADLIYRKCEGLSNWYGLIPELFPNAKY 300

Query: 301 IYGIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGY 360
           IYGIMTGSMEPYLKKLRHYAG L LMSADYGSSEGWVGANVNPMLPPEMATF VLPNIGY
Sbjct: 301 IYGIMTGSMEPYLKKLRHYAGHLPLMSADYGSSEGWVGANVNPMLPPEMATFAVLPNIGY 360

Query: 361 FEFIPLKESAEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVK 420
           FEFIPLKE+A+ Q Q          NKP+GLTEVK+GEEYEIIVTNVAGLYRYRLGD VK
Sbjct: 361 FEFIPLKENAQGQHQ---------RNKPIGLTEVKIGEEYEIIVTNVAGLYRYRLGDAVK 420

Query: 421 VMGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDM 480
           VMGFHN+TPE+KF+CRRNLLLSINIDK TEKDLQ+AVEAAG++LA EKLEVVDFTS+VD+
Sbjct: 421 VMGFHNSTPELKFICRRNLLLSINIDKITEKDLQLAVEAAGNVLAAEKLEVVDFTSYVDV 480

Query: 481 SREPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTF 540
           SREPGHYVIFWE SGEAKGEVLGEC NCLDRAFLDAGY+SSRKVNAIGALELRVVRKGTF
Sbjct: 481 SREPGHYVIFWEISGEAKGEVLGECSNCLDRAFLDAGYMSSRKVNAIGALELRVVRKGTF 540

Query: 541 HKIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFST 586
           HKIMDH+LSLGAAVSQYKTPRCVIPTNT VLQILC+NVVNSYFST
Sbjct: 541 HKIMDHHLSLGAAVSQYKTPRCVIPTNTAVLQILCSNVVNSYFST 573

BLAST of MS005872 vs. TAIR 10
Match: AT2G46370.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 745.7 bits (1924), Expect = 2.8e-215
Identity = 366/588 (62.24%), Postives = 457/588 (77.72%), Query Frame = 0

Query: 1   MLDKMEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGR-TDP-QS 60
           ML+K+E FD  +VI++F+EMT++A +VQ++TL++IL +N SA YLQ+ GLNG  TDP ++
Sbjct: 1   MLEKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATDPEEA 60

Query: 61  FKDCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLRYFLQICAEEVLLLIPSIGDF 120
           FK  VPLVT  +LEPYI+R+ DG TSPILTG P+  ISL         + +     + + 
Sbjct: 61  FKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMEN 120

Query: 121 VAVPFLRRFLFFFLFYREFPI-RNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKC 180
               F   F F     R+FPI  NGKALQFI+SSKQ+ + GG+  GTATTNVYR+  FK 
Sbjct: 121 TLQLFRTAFAF---RNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKA 180

Query: 181 TMKAIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELV 240
            MK+I S  CSPDEVIF PD HQ+LYCHLL G++FRD+V++VF+ FAH +VHAFRTFE V
Sbjct: 181 GMKSITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQV 240

Query: 241 WEELCSNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPN 300
           WEE+ ++I+DGVLS+ +T P++R AMSKLL PNPELA+ I  KC  LSNWYGLIP LFPN
Sbjct: 241 WEEIVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSLSNWYGLIPALFPN 300

Query: 301 AKYIYGIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPN 360
           AKY+YGIMTGSMEPY+ KLRHYAGDL L+S DYGSSEGW+ ANV P L PE ATF V+PN
Sbjct: 301 AKYVYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPN 360

Query: 361 IGYFEFIPLKESAEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGD 420
           +GYFEF+P+ E+ E             E KPVGLT+VK+GEEYE+++TN AGLYRYRLGD
Sbjct: 361 LGYFEFLPVSETGE------------GEEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGD 420

Query: 421 VVKVMGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSH 480
           VVKV+GF+N TP++KF+CRRNL+LSINIDKNTE+DLQ++VE+A   L+EEK+EV+DF+S+
Sbjct: 421 VVKVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEKIEVIDFSSY 480

Query: 481 VDMSREPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRK 540
           +D+S +PGHY IFWE SGE   +VL +CCNCLDRAF+DAGYVSSRK   IGALELRVV K
Sbjct: 481 IDVSTDPGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAK 540

Query: 541 GTFHKIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFST 586
           GTF KI +H+L LG++  Q+K PRCV P+N  VLQILC NVV+SYFST
Sbjct: 541 GTFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFST 573

BLAST of MS005872 vs. TAIR 10
Match: AT2G46370.2 (Auxin-responsive GH3 family protein )

HSP 1 Score: 745.7 bits (1924), Expect = 2.8e-215
Identity = 366/588 (62.24%), Postives = 457/588 (77.72%), Query Frame = 0

Query: 1   MLDKMEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGR-TDP-QS 60
           ML+K+E FD  +VI++F+EMT++A +VQ++TL++IL +N SA YLQ+ GLNG  TDP ++
Sbjct: 1   MLEKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATDPEEA 60

Query: 61  FKDCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLRYFLQICAEEVLLLIPSIGDF 120
           FK  VPLVT  +LEPYI+R+ DG TSPILTG P+  ISL         + +     + + 
Sbjct: 61  FKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMEN 120

Query: 121 VAVPFLRRFLFFFLFYREFPI-RNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKC 180
               F   F F     R+FPI  NGKALQFI+SSKQ+ + GG+  GTATTNVYR+  FK 
Sbjct: 121 TLQLFRTAFAF---RNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKA 180

Query: 181 TMKAIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELV 240
            MK+I S  CSPDEVIF PD HQ+LYCHLL G++FRD+V++VF+ FAH +VHAFRTFE V
Sbjct: 181 GMKSITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQV 240

Query: 241 WEELCSNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPN 300
           WEE+ ++I+DGVLS+ +T P++R AMSKLL PNPELA+ I  KC  LSNWYGLIP LFPN
Sbjct: 241 WEEIVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSLSNWYGLIPALFPN 300

Query: 301 AKYIYGIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPN 360
           AKY+YGIMTGSMEPY+ KLRHYAGDL L+S DYGSSEGW+ ANV P L PE ATF V+PN
Sbjct: 301 AKYVYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPN 360

Query: 361 IGYFEFIPLKESAEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGD 420
           +GYFEF+P+ E+ E             E KPVGLT+VK+GEEYE+++TN AGLYRYRLGD
Sbjct: 361 LGYFEFLPVSETGE------------GEEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGD 420

Query: 421 VVKVMGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSH 480
           VVKV+GF+N TP++KF+CRRNL+LSINIDKNTE+DLQ++VE+A   L+EEK+EV+DF+S+
Sbjct: 421 VVKVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEKIEVIDFSSY 480

Query: 481 VDMSREPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRK 540
           +D+S +PGHY IFWE SGE   +VL +CCNCLDRAF+DAGYVSSRK   IGALELRVV K
Sbjct: 481 IDVSTDPGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAK 540

Query: 541 GTFHKIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFST 586
           GTF KI +H+L LG++  Q+K PRCV P+N  VLQILC NVV+SYFST
Sbjct: 541 GTFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFST 573

BLAST of MS005872 vs. TAIR 10
Match: AT2G46370.4 (Auxin-responsive GH3 family protein )

HSP 1 Score: 745.7 bits (1924), Expect = 2.8e-215
Identity = 366/588 (62.24%), Postives = 457/588 (77.72%), Query Frame = 0

Query: 1   MLDKMEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGR-TDP-QS 60
           ML+K+E FD  +VI++F+EMT++A +VQ++TL++IL +N SA YLQ+ GLNG  TDP ++
Sbjct: 12  MLEKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATDPEEA 71

Query: 61  FKDCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLRYFLQICAEEVLLLIPSIGDF 120
           FK  VPLVT  +LEPYI+R+ DG TSPILTG P+  ISL         + +     + + 
Sbjct: 72  FKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMEN 131

Query: 121 VAVPFLRRFLFFFLFYREFPI-RNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKC 180
               F   F F     R+FPI  NGKALQFI+SSKQ+ + GG+  GTATTNVYR+  FK 
Sbjct: 132 TLQLFRTAFAF---RNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKA 191

Query: 181 TMKAIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELV 240
            MK+I S  CSPDEVIF PD HQ+LYCHLL G++FRD+V++VF+ FAH +VHAFRTFE V
Sbjct: 192 GMKSITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQV 251

Query: 241 WEELCSNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPN 300
           WEE+ ++I+DGVLS+ +T P++R AMSKLL PNPELA+ I  KC  LSNWYGLIP LFPN
Sbjct: 252 WEEIVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSLSNWYGLIPALFPN 311

Query: 301 AKYIYGIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPN 360
           AKY+YGIMTGSMEPY+ KLRHYAGDL L+S DYGSSEGW+ ANV P L PE ATF V+PN
Sbjct: 312 AKYVYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPN 371

Query: 361 IGYFEFIPLKESAEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGD 420
           +GYFEF+P+ E+ E             E KPVGLT+VK+GEEYE+++TN AGLYRYRLGD
Sbjct: 372 LGYFEFLPVSETGE------------GEEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGD 431

Query: 421 VVKVMGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSH 480
           VVKV+GF+N TP++KF+CRRNL+LSINIDKNTE+DLQ++VE+A   L+EEK+EV+DF+S+
Sbjct: 432 VVKVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEKIEVIDFSSY 491

Query: 481 VDMSREPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRK 540
           +D+S +PGHY IFWE SGE   +VL +CCNCLDRAF+DAGYVSSRK   IGALELRVV K
Sbjct: 492 IDVSTDPGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAK 551

Query: 541 GTFHKIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFST 586
           GTF KI +H+L LG++  Q+K PRCV P+N  VLQILC NVV+SYFST
Sbjct: 552 GTFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFST 584

BLAST of MS005872 vs. TAIR 10
Match: AT2G46370.3 (Auxin-responsive GH3 family protein )

HSP 1 Score: 665.6 bits (1716), Expect = 3.6e-191
Identity = 321/509 (63.06%), Postives = 393/509 (77.21%), Query Frame = 0

Query: 78  IADGATSPILTGKPIKTISLRYFLQICAEEVLLLIPSIGDFVAVPFLRRFLFFFLFYREF 137
           + DG TSPILTG P+  ISL         + +     + +     F   F F     R+F
Sbjct: 1   MVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTLQLFRTAFAF---RNRDF 60

Query: 138 PI-RNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMKAIQSQCCSPDEVIFGP 197
           PI  NGKALQFI+SSKQ+ + GG+  GTATTNVYR+  FK  MK+I S  CSPDEVIF P
Sbjct: 61  PIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSITSPSCSPDEVIFSP 120

Query: 198 DFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEELCSNIRDGVLSSWVTA 257
           D HQ+LYCHLL G++FRD+V++VF+ FAH +VHAFRTFE VWEE+ ++I+DGVLS+ +T 
Sbjct: 121 DVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIVTDIKDGVLSNRITV 180

Query: 258 PTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYGIMTGSMEPYLKKL 317
           P++R AMSKLL PNPELA+ I  KC  LSNWYGLIP LFPNAKY+YGIMTGSMEPY+ KL
Sbjct: 181 PSVRTAMSKLLTPNPELAETIRTKCMSLSNWYGLIPALFPNAKYVYGIMTGSMEPYVPKL 240

Query: 318 RHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFEFIPLKESAEEQKQN 377
           RHYAGDL L+S DYGSSEGW+ ANV P L PE ATF V+PN+GYFEF+P+ E+ E     
Sbjct: 241 RHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGYFEFLPVSETGE----- 300

Query: 378 EDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVKVMGFHNATPEVKFVCR 437
                   E KPVGLT+VK+GEEYE+++TN AGLYRYRLGDVVKV+GF+N TP++KF+CR
Sbjct: 301 -------GEEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVKVIGFYNNTPQLKFICR 360

Query: 438 RNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDMSREPGHYVIFWETSGE 497
           RNL+LSINIDKNTE+DLQ++VE+A   L+EEK+EV+DF+S++D+S +PGHY IFWE SGE
Sbjct: 361 RNLILSINIDKNTERDLQLSVESAAKRLSEEKIEVIDFSSYIDVSTDPGHYAIFWEISGE 420

Query: 498 AKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTFHKIMDHYLSLGAAVSQ 557
              +VL +CCNCLDRAF+DAGYVSSRK   IGALELRVV KGTF KI +H+L LG++  Q
Sbjct: 421 TNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTFRKIQEHFLGLGSSAGQ 480

Query: 558 YKTPRCVIPTNTTVLQILCANVVNSYFST 586
           +K PRCV P+N  VLQILC NVV+SYFST
Sbjct: 481 FKMPRCVKPSNAKVLQILCENVVSSYFST 494

BLAST of MS005872 vs. TAIR 10
Match: AT4G03400.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 531.9 bits (1369), Expect = 6.3e-151
Identity = 291/598 (48.66%), Postives = 387/598 (64.72%), Query Frame = 0

Query: 11  EKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQS-LG------LNGRTDPQSFKDCV 70
           + VI  FE ++++A +VQ ETL +ILE N   EYL+  LG      ++  T    F   V
Sbjct: 9   DDVIGWFEHVSENACKVQSETLRRILELNSGVEYLRKWLGTVDVEKMDDYTLETLFTSLV 68

Query: 71  PLVTHNDLEPYIQRIADGATSPILTGKPIKTISL---------RY--FLQICAEEVLLLI 130
           P+V+H DL+PYIQRIADG TSP+LT +PI  +SL         +Y  F +  A+  L + 
Sbjct: 69  PIVSHADLDPYIQRIADGETSPLLTQEPITVLSLSSGTTEGRQKYVPFTRHSAQTTLQIF 128

Query: 131 PSIGDFVAVPFLRRFLFFFLFYREFPIR-NGKALQFIYSSKQFKTKGGLAAGTATTNVYR 190
                 ++  +  RF         +PIR  G+ L+FIY+ K+FKT GGL  GTATT+ Y 
Sbjct: 129 R-----LSAAYRSRF---------YPIREGGRILEFIYAGKEFKTLGGLTVGTATTHYYA 188

Query: 191 SAKFKCTMKAIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAF 250
           S +FK   +  +S  CSP EVI G DF Q  YCHLL GL +  +VEFV S F+++IV AF
Sbjct: 189 SEEFKTKQETTKSFTCSPQEVISGGDFGQCTYCHLLLGLHYSSQVEFVASAFSYTIVQAF 248

Query: 251 RTFELVWEELCSNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLS---NWY 310
             FE +W E+C++I++G LSS +T P +R A+  L+RPNP LA  I   C  L     W+
Sbjct: 249 SFFEEIWREICADIKEGNLSSRITLPKMRKAVLALIRPNPSLASHIEEICLELETNLGWF 308

Query: 311 GLIPELFPNAKYIYGIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPE 370
           GLI +L+PNAK+I  IMTGSM PYL KLRHYAG L L+SADYGS+E W+G NV+P LPPE
Sbjct: 309 GLISKLWPNAKFISSIMTGSMLPYLNKLRHYAGGLPLVSADYGSTESWIGVNVDPHLPPE 368

Query: 371 MATFVVLPNIGYFEFIPLKESAEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVA 430
             +F V+P   YFEFIPL     +     DG  V  E+KPV L++VK+G+EYE+++T   
Sbjct: 369 DVSFAVIPTFSYFEFIPLYRRQNQSDICIDGDFV--EDKPVPLSQVKLGQEYELVLTTFT 428

Query: 431 GLYRYRLGDVVKVMGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEE- 490
           GLYRYRLGDVV+V  FH  TP++ F+ RR L+L+INIDKNTEKDLQ  V+ A  LL+   
Sbjct: 429 GLYRYRLGDVVEVTSFHKGTPKLSFIYRRKLILTINIDKNTEKDLQRVVDKASQLLSRST 488

Query: 491 KLEVVDFTSHVDMSREPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAI 550
           + EVVDFTSH D+   PGHYVI+WE  GEA  + L ECC  +D AF+D GYV SR++N+I
Sbjct: 489 RAEVVDFTSHADVIARPGHYVIYWEIRGEADDKALEECCREMDTAFVDYGYVVSRRMNSI 548

Query: 551 GALELRVVRKGTFHKIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFST 586
           G LELRVV +GTF K+ +  +     ++Q+KTPRC   TN+ +L IL  + +  + S+
Sbjct: 549 GPLELRVVERGTFGKVAERCVGKCGGLNQFKTPRCT--TNSVMLDILNDSTIKRFRSS 588

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022147427.10.0e+0094.02jasmonic acid-amido synthetase JAR1-like [Momordica charantia] >XP_022147428.1 j... [more]
XP_023539820.15.3e-28585.35jasmonic acid-amido synthetase JAR1 [Cucurbita pepo subsp. pepo] >XP_023539821.1... [more]
XP_022974649.11.5e-28485.35jasmonic acid-amido synthetase JAR1 [Cucurbita maxima] >XP_022974650.1 jasmonic ... [more]
KAG6597096.12.6e-28485.18Jasmonoyl--L-amino acid synthetase JAR6, partial [Cucurbita argyrosperma subsp. ... [more]
XP_022941396.12.6e-28485.18jasmonic acid-amido synthetase JAR1 [Cucurbita moschata] >XP_022941403.1 jasmoni... [more]
Match NameE-valueIdentityDescription
A0A1J6KGJ97.2e-24571.62Jasmonoyl--L-amino acid synthetase JAR4 OS=Nicotiana attenuata OX=49451 GN=JAR4 ... [more]
A0A314KSQ46.8e-24371.89Jasmonoyl--L-amino acid synthetase JAR6 OS=Nicotiana attenuata OX=49451 GN=JAR6 ... [more]
Q6I5813.4e-21864.24Jasmonoyl--L-amino acid synthetase GH3.5 OS=Oryza sativa subsp. japonica OX=3994... [more]
Q9SKE23.9e-21462.24Jasmonoyl--L-amino acid synthetase JAR1 OS=Arabidopsis thaliana OX=3702 GN=JAR1 ... [more]
Q53P496.9e-16350.50Probable indole-3-acetic acid-amido synthetase GH3.12 OS=Oryza sativa subsp. jap... [more]
Match NameE-valueIdentityDescription
A0A6J1D2B90.0e+0094.02jasmonic acid-amido synthetase JAR1-like OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A6J1II847.4e-28585.35jasmonic acid-amido synthetase JAR1 OS=Cucurbita maxima OX=3661 GN=LOC111473333 ... [more]
A0A6J1FL001.3e-28485.18jasmonic acid-amido synthetase JAR1 OS=Cucurbita moschata OX=3662 GN=LOC11144669... [more]
A0A5A7U0I32.6e-28280.58Jasmonic acid-amido synthetase JAR1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6... [more]
A0A1S4DTP65.9e-28284.27jasmonic acid-amido synthetase JAR1 OS=Cucumis melo OX=3656 GN=LOC103483208 PE=3... [more]
Match NameE-valueIdentityDescription
AT2G46370.12.8e-21562.24Auxin-responsive GH3 family protein [more]
AT2G46370.22.8e-21562.24Auxin-responsive GH3 family protein [more]
AT2G46370.42.8e-21562.24Auxin-responsive GH3 family protein [more]
AT2G46370.33.6e-19163.06Auxin-responsive GH3 family protein [more]
AT4G03400.16.3e-15148.66Auxin-responsive GH3 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 367..387
NoneNo IPR availablePANTHERPTHR31901:SF40JASMONIC ACID-AMIDO SYNTHETASE JAR1-LIKE ISOFORM X1coord: 7..573
IPR004993GH3 familyPFAMPF03321GH3coord: 16..563
e-value: 5.3E-172
score: 573.4
IPR004993GH3 familyPANTHERPTHR31901GH3 DOMAIN-CONTAINING PROTEINcoord: 7..573

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS005872.1MS005872.1mRNA