MS005672 (gene) Bitter gourd (TR) v1

Overview


Name	MS005672
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Malic enzyme
Location	scaffold254: 696964 .. 697117 (+)
RNA-Seq Expression	MS005672
Synteny	MS005672

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTTTGATTGCTCACATTGAGGAGTATGCAGCAATTGTATTACACAGCAACTGTAGGTCTCGTTTGCCAGAATTATGGTGGCTTGTTTAGAAGACCAAGGGGAATGTATTTCAGTGCAGCCGATCGTGGAGAAATGATGTCTATGGTTTATAAT

mRNA sequence

GTTTTGATTGCTCACATTGAGGAGTATGCAGCAATTTACACAGCAACTGTAGGTCTCGTTTGCCAGAATTATGGTGGCTTGTTTAGAAGACCAAGGGGAATGTATTTCAGTGCAGCCGATCGTGGAGAAATGATGTCTATGGTTTATAAT

Coding sequence (CDS)

GTTTTGATTGCTCACATTGAGGAGTATGCAGCAATTTACACAGCAACTGTAGGTCTCGTTTGCCAGAATTATGGTGGCTTGTTTAGAAGACCAAGGGGAATGTATTTCAGTGCAGCCGATCGTGGAGAAATGATGTCTATGGTTTATAAT

Protein sequence

VLIAHIEEYAAIYTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN

Homology

[Close]

BLAST of MS005672 vs. NCBI nr
Match: XP_038875079.1 (NAD-dependent malic enzyme 62 kDa isoform, mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 94.7 bits (234), Expect = 2.3e-16
Identity = 47/51 (92.16%), Postives = 47/51 (92.16%), Query Frame = 0


Query: 1   VLIAHIEEYAAI-YTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 51
           VLIAHIEEYA I YT TVGLVCQNYGGLFRRPRGMYFSA DRGEMMSMVYN
Sbjct: 133 VLIAHIEEYAPIVYTPTVGLVCQNYGGLFRRPRGMYFSAEDRGEMMSMVYN 183

[Close]

BLAST of MS005672 vs. NCBI nr
Match: XP_038875085.1 (NAD-dependent malic enzyme 62 kDa isoform, mitochondrial isoform X2 [Benincasa hispida] >XP_038875089.1 NAD-dependent malic enzyme 62 kDa isoform, mitochondrial isoform X2 [Benincasa hispida] >XP_038875091.1 NAD-dependent malic enzyme 62 kDa isoform, mitochondrial isoform X2 [Benincasa hispida])

HSP 1 Score: 94.7 bits (234), Expect = 2.3e-16
Identity = 47/51 (92.16%), Postives = 47/51 (92.16%), Query Frame = 0


Query: 1   VLIAHIEEYAAI-YTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 51
           VLIAHIEEYA I YT TVGLVCQNYGGLFRRPRGMYFSA DRGEMMSMVYN
Sbjct: 68  VLIAHIEEYAPIVYTPTVGLVCQNYGGLFRRPRGMYFSAEDRGEMMSMVYN 118

[Close]

BLAST of MS005672 vs. NCBI nr
Match: XP_022147088.1 (NAD-dependent malic enzyme 62 kDa isoform, mitochondrial [Momordica charantia])

HSP 1 Score: 94.4 bits (233), Expect = 3.1e-16
Identity = 47/51 (92.16%), Postives = 47/51 (92.16%), Query Frame = 0


Query: 1   VLIAHIEEYAAI-YTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 51
           VLIAHIEEYA I YT TVGLVCQNY GLFRRPRGMYFSAADRGEMMSMVYN
Sbjct: 133 VLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAADRGEMMSMVYN 183

[Close]

BLAST of MS005672 vs. NCBI nr
Match: KCW73248.1 (hypothetical protein EUGRSUZ_E017132 [Eucalyptus grandis])

HSP 1 Score: 92.4 bits (228), Expect = 1.2e-15
Identity = 46/51 (90.20%), Postives = 48/51 (94.12%), Query Frame = 0


Query: 1   VLIAHIEEYAAI-YTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 51
           VLIA+I+EYA I YT TVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN
Sbjct: 137 VLIANIKEYAPIVYTPTVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 187

[Close]

BLAST of MS005672 vs. NCBI nr
Match: KAF8028675.1 (hypothetical protein BT93_E1352 [Corymbia citriodora subsp. variegata])

HSP 1 Score: 92.4 bits (228), Expect = 1.2e-15
Identity = 46/51 (90.20%), Postives = 48/51 (94.12%), Query Frame = 0


Query: 1   VLIAHIEEYAAI-YTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 51
           VLIA+I+EYA I YT TVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN
Sbjct: 140 VLIANIKEYAPIVYTPTVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 190

[Close]

BLAST of MS005672 vs. ExPASy Swiss-Prot
Match: Q9SIU0 (NAD-dependent malic enzyme 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NAD-ME1 PE=1 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 4.9e-17
Identity = 44/51 (86.27%), Postives = 45/51 (88.24%), Query Frame = 0


Query: 1   VLIAHIEEYAAI-YTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 51
           VLI +IEEYA I YT TVGLVCQNY GLFRRPRGMYFSA DRGEMMSMVYN
Sbjct: 130 VLINNIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYN 180

[Close]

BLAST of MS005672 vs. ExPASy Swiss-Prot
Match: P37221 (NAD-dependent malic enzyme 62 kDa isoform, mitochondrial OS=Solanum tuberosum OX=4113 PE=1 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 4.1e-16
Identity = 42/51 (82.35%), Postives = 44/51 (86.27%), Query Frame = 0


Query: 1   VLIAHIEEYAAI-YTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 51
           VL+ +IEEYA I YT TVGLVCQ Y GLFRRPRGMYFSA DRGEMMSMVYN
Sbjct: 133 VLMENIEEYAPIVYTPTVGLVCQKYSGLFRRPRGMYFSAEDRGEMMSMVYN 183

[Close]

BLAST of MS005672 vs. ExPASy Swiss-Prot
Match: Q8L7K9 (NAD-dependent malic enzyme 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NAD-ME2 PE=1 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 6.0e-15
Identity = 38/51 (74.51%), Postives = 45/51 (88.24%), Query Frame = 0


Query: 1   VLIAHIEEYA-AIYTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 51
           VLI +I+++A  IYT TVGLVCQNY GL+RRPRGMYFSA D+GEMMSM+YN
Sbjct: 123 VLIDNIKDFAPIIYTPTVGLVCQNYSGLYRRPRGMYFSAKDKGEMMSMIYN 173

[Close]

BLAST of MS005672 vs. ExPASy Swiss-Prot
Match: P37225 (NAD-dependent malic enzyme 59 kDa isoform, mitochondrial OS=Solanum tuberosum OX=4113 PE=1 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 7.8e-15
Identity = 38/51 (74.51%), Postives = 45/51 (88.24%), Query Frame = 0


Query: 1   VLIAHIEEYA-AIYTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 51
           VLI +I+++A  IYT TVGLVCQNY GLFRRPRGMYFSA D+GEMMSM++N
Sbjct: 116 VLIDNIKDFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIFN 166

[Close]

BLAST of MS005672 vs. ExPASy Swiss-Prot
Match: P37224 (NAD-dependent malic enzyme 65 kDa isoform, mitochondrial OS=Amaranthus hypochondriacus OX=28502 PE=1 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 3.0e-14
Identity = 39/51 (76.47%), Postives = 43/51 (84.31%), Query Frame = 0


Query: 1   VLIAHIEEYAAIY-TATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 51
           VLI +IEEYA I  T TVGLVCQ + GL+RRPRGMYFS+ DRGEMMSMVYN
Sbjct: 130 VLIENIEEYAPIVSTPTVGLVCQKFSGLYRRPRGMYFSSDDRGEMMSMVYN 180

[Close]

BLAST of MS005672 vs. ExPASy TrEMBL
Match: A0A6J1D0B1 (Malic enzyme OS=Momordica charantia OX=3673 GN=LOC111016110 PE=3 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 1.5e-16
Identity = 47/51 (92.16%), Postives = 47/51 (92.16%), Query Frame = 0


Query: 1   VLIAHIEEYAAI-YTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 51
           VLIAHIEEYA I YT TVGLVCQNY GLFRRPRGMYFSAADRGEMMSMVYN
Sbjct: 133 VLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAADRGEMMSMVYN 183

[Close]

BLAST of MS005672 vs. ExPASy TrEMBL
Match: A0A059C4F9 (Malic enzyme (Fragment) OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_E017132 PE=3 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 5.6e-16
Identity = 46/51 (90.20%), Postives = 48/51 (94.12%), Query Frame = 0


Query: 1   VLIAHIEEYAAI-YTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 51
           VLIA+I+EYA I YT TVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN
Sbjct: 137 VLIANIKEYAPIVYTPTVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 187

[Close]

BLAST of MS005672 vs. ExPASy TrEMBL
Match: A0A059C453 (Malic enzyme OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_E017132 PE=3 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 5.6e-16
Identity = 46/51 (90.20%), Postives = 48/51 (94.12%), Query Frame = 0


Query: 1   VLIAHIEEYAAI-YTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 51
           VLIA+I+EYA I YT TVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN
Sbjct: 137 VLIANIKEYAPIVYTPTVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 187

[Close]

BLAST of MS005672 vs. ExPASy TrEMBL
Match: A0A6J1IYS7 (Malic enzyme OS=Cucurbita maxima OX=3661 GN=LOC111479676 PE=3 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 5.6e-16
Identity = 46/51 (90.20%), Postives = 46/51 (90.20%), Query Frame = 0


Query: 1   VLIAHIEEYAAI-YTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 51
           VLIAHIEEYA I YT TVGLVCQNY GLFRRPRGMYFSA DRGEMMSMVYN
Sbjct: 68  VLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYN 118

[Close]

BLAST of MS005672 vs. ExPASy TrEMBL
Match: A0A6J1IQW3 (Malic enzyme OS=Cucurbita maxima OX=3661 GN=LOC111479676 PE=3 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 5.6e-16
Identity = 46/51 (90.20%), Postives = 46/51 (90.20%), Query Frame = 0


Query: 1   VLIAHIEEYAAI-YTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 51
           VLIAHIEEYA I YT TVGLVCQNY GLFRRPRGMYFSA DRGEMMSMVYN
Sbjct: 133 VLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYN 183

[Close]

BLAST of MS005672 vs. TAIR 10
Match: AT2G13560.1 (NAD-dependent malic enzyme 1 )

HSP 1 Score: 87.4 bits (215), Expect = 3.5e-18
Identity = 44/51 (86.27%), Postives = 45/51 (88.24%), Query Frame = 0


Query: 1   VLIAHIEEYAAI-YTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 51
           VLI +IEEYA I YT TVGLVCQNY GLFRRPRGMYFSA DRGEMMSMVYN
Sbjct: 130 VLINNIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYN 180

[Close]

BLAST of MS005672 vs. TAIR 10
Match: AT4G00570.1 (NAD-dependent malic enzyme 2 )

HSP 1 Score: 80.5 bits (197), Expect = 4.3e-16
Identity = 38/51 (74.51%), Postives = 45/51 (88.24%), Query Frame = 0


Query: 1   VLIAHIEEYA-AIYTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 51
           VLI +I+++A  IYT TVGLVCQNY GL+RRPRGMYFSA D+GEMMSM+YN
Sbjct: 123 VLIDNIKDFAPIIYTPTVGLVCQNYSGLYRRPRGMYFSAKDKGEMMSMIYN 173

[Close]

BLAST of MS005672 vs. TAIR 10
Match: AT5G25880.1 (NADP-malic enzyme 3 )

HSP 1 Score: 50.8 bits (120), Expect = 3.6e-07
Identity = 21/51 (41.18%), Postives = 36/51 (70.59%), Query Frame = 0


Query: 1   VLIAHIEE-YAAIYTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 51
           +LI ++EE    +YT TVG  CQ YG ++RRP+G+Y S  ++G+++ ++ N
Sbjct: 123 LLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLYISLKEKGKILEVLKN 173

[Close]

BLAST of MS005672 vs. TAIR 10
Match: AT2G19900.1 (NADP-malic enzyme 1 )

HSP 1 Score: 50.4 bits (119), Expect = 4.7e-07
Identity = 22/51 (43.14%), Postives = 36/51 (70.59%), Query Frame = 0


Query: 1   VLIAHIEEYAAI-YTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 51
           +LI ++EE   I YT TVG  CQ +G +FRRP+G++ S  D+G+++ ++ N
Sbjct: 116 LLIDNVEELLPIVYTPTVGEACQKFGSIFRRPQGLFISLKDKGKILDVLKN 166

[Close]

BLAST of MS005672 vs. TAIR 10
Match: AT5G11670.1 (NADP-malic enzyme 2 )

HSP 1 Score: 50.4 bits (119), Expect = 4.7e-07
Identity = 21/51 (41.18%), Postives = 36/51 (70.59%), Query Frame = 0


Query: 1   VLIAHIEE-YAAIYTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 51
           +LI ++EE    +YT TVG  CQ YG +FR+P+G+Y S  ++G+++ ++ N
Sbjct: 123 LLIDNVEELLPVVYTPTVGEACQKYGSIFRKPQGLYISLNEKGKILEVLKN 173

The following BLAST results are available for this feature:

BLAST of MS005672 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038875079.1	2.3e-16	92.16	NAD-dependent malic enzyme 62 kDa isoform, mitochondrial isoform X1 [Benincasa h...	[more]
XP_038875085.1	2.3e-16	92.16	NAD-dependent malic enzyme 62 kDa isoform, mitochondrial isoform X2 [Benincasa h...	[more]
XP_022147088.1	3.1e-16	92.16	NAD-dependent malic enzyme 62 kDa isoform, mitochondrial [Momordica charantia]	[more]
KCW73248.1	1.2e-15	90.20	hypothetical protein EUGRSUZ_E017132 [Eucalyptus grandis]	[more]
KAF8028675.1	1.2e-15	90.20	hypothetical protein BT93_E1352 [Corymbia citriodora subsp. variegata]	[more]

BLAST of MS005672 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SIU0	4.9e-17	86.27	NAD-dependent malic enzyme 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=N...	[more]
P37221	4.1e-16	82.35	NAD-dependent malic enzyme 62 kDa isoform, mitochondrial OS=Solanum tuberosum OX...	[more]
Q8L7K9	6.0e-15	74.51	NAD-dependent malic enzyme 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=N...	[more]
P37225	7.8e-15	74.51	NAD-dependent malic enzyme 59 kDa isoform, mitochondrial OS=Solanum tuberosum OX...	[more]
P37224	3.0e-14	76.47	NAD-dependent malic enzyme 65 kDa isoform, mitochondrial OS=Amaranthus hypochond...	[more]

BLAST of MS005672 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D0B1	1.5e-16	92.16	Malic enzyme OS=Momordica charantia OX=3673 GN=LOC111016110 PE=3 SV=1	[more]
A0A059C4F9	5.6e-16	90.20	Malic enzyme (Fragment) OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_E017132 PE=3 S...	[more]
A0A059C453	5.6e-16	90.20	Malic enzyme OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_E017132 PE=3 SV=1	[more]
A0A6J1IYS7	5.6e-16	90.20	Malic enzyme OS=Cucurbita maxima OX=3661 GN=LOC111479676 PE=3 SV=1	[more]
A0A6J1IQW3	5.6e-16	90.20	Malic enzyme OS=Cucurbita maxima OX=3661 GN=LOC111479676 PE=3 SV=1	[more]

BLAST of MS005672 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G13560.1	3.5e-18	86.27	NAD-dependent malic enzyme 1	[more]
AT4G00570.1	4.3e-16	74.51	NAD-dependent malic enzyme 2	[more]
AT5G25880.1	3.6e-07	41.18	NADP-malic enzyme 3	[more]
AT2G19900.1	4.7e-07	43.14	NADP-malic enzyme 1	[more]
AT5G11670.1	4.7e-07	41.18	NADP-malic enzyme 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR037062	Malic enzyme, N-terminal domain superfamily	GENE3D	3.40.50.10380		coord: 1..50 e-value: 2.2E-14 score: 55.2
IPR012301	Malic enzyme, N-terminal domain	PFAM	PF00390	malic	coord: 3..50 e-value: 1.8E-8 score: 34.4
None	No IPR available	PANTHER	PTHR23406:SF32	NAD-DEPENDENT MALIC ENZYME 1, MITOCHONDRIAL	coord: 1..50
None	No IPR available	PANTHER	PTHR23406	MALIC ENZYME-RELATED	coord: 1..50
None	No IPR available	SUPERFAMILY	53223	Aminoacid dehydrogenase-like, N-terminal domain	coord: 5..50

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS005672.1	MS005672.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004471	malate dehydrogenase (decarboxylating) (NAD+) activity