MS005299 (gene) Bitter gourd (TR) v1

Overview


Name	MS005299
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Calmodulin-like
Location	scaffold83: 707629 .. 710881 (+)
RNA-Seq Expression	MS005299
Synteny	MS005299

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTATTTGGGATCGATGATATTATAGGGTGCATCACGATCAAAGAGTTGGGCACAGCGATTAGATCATTAGATCAAAATCCCACTGAATTAGAACTGCAGACCATGATGAATGAAGTTGATGTCAATGGGAATGGATCCATTGAATTTGGAGAGTTCTTCAATCTCTTGGCAAAAAAGATGAAGGTAACTATATATTTATATATCATCTTTTGTTATGAACATGTAATCCCAATTGTGTAATTTTATTTTTTCGAGTAGGGAATTTGAATATATCTAACCTTTTAAGTGTGTTGACTAATATAATTGAGCTTGGTTCGTTGGACCAACATTTTTAATTGAGAAAAAAATAATGTCATTAGTAATGCAAGATTTATCTTGCATAATTTTTTTAATACAACATGTGGGAGTCGGGGATCGAACTTACAACCTCTTGATTGGAGATATATGTTAATTATCGTTGCGTTTGACTCGCTTGGACTTTTTTATCATACTGTAAAATTTTATCTTTTACTTGTAAGGAATTTTCATATAAAATTTATATGCATCAATTAAATTAATTTGCTCTATACTTCTGTTTGCCGTTTTATTATCAATATAATTTTCAACCAGCCTTATGAGCTCCAATTTCTTGGCATATAAGTTTTGGCTTATATTTTTATTTCAATGATTTATGGAAATCTGTAGCTTGTTAATAAAGCAAGTCAATTATCTTTTTAAATAAGCATCATTATATTTTCAATAATTATAGTTAAACAAAAATGATCAAATCTTCATTTTGTTCTGATAAAGTAAGCAAGCATGTTTTATATTTAAGAATCTGATAAATAAATTAATTAAATGCAATATGTTGATAGTTTAATATGATAATTAATTAGTTTGGCACGTAATATTAACATTCAATAGTTTTTAGATCCTATTAAGTAATTATGTAGGATATATGATATCAAGTATAAGTAGAATTCAATCGACATGAACGTAGCTCAATGCTTAAAGTATTCACCGATTTGAAGGTTTGAATAAAAAATGAAAAAATAATAATTCAATGGTGGCTTGATTGAGTGATTTTCTAGCAATTTCCTAGGATGTGAATACGTGAATATGATCTTCAAGAGTTAGAAATTATGAATAAAGAATCTCAAATATATGAATTAAGATAATATTATACCTTTCTTCGTACAAACATTTTAGTAAATTTCTAAAATTTAAACGACGTACCTTAGGTTAAAGTGAATAATATCATATATCATATAAATAAAACATAAGTCAATTGTCCATTAGGCATTTATATGACTTGATATAGATAATACTTTGTTCCTTGTCGGTTGAATTTTGTTTCATTTACTTGTCATGGTTAGTCGTATATGAGCCAGTTTACATTTTTGTTTAAATCCTATTTTGATTCTCGTACTATTTTAGTTTTTGTTTCATTTTAGTCTCTATATTTTTAAAATGTTAATTTTGGTTATTCCTATATTTTCATTTTTTTTGTCTATTTTAATTCTTATATTCTGAAAATATTTATTTTTGTTACTATACTTTTAAAAAGAGATTATTTTAGTCTCTGTACGAAAATTTTAATACAAAAAATAAAAGAGAGAGACAAAAATAATTTTTAAAAACGGAAGTACAAACTTAAAATAAATAAAAACTTAAAAACTTTATGCGTAGATCGAAATAAACAAAAACATAAAAGTAAACCAAAATGATTTAAAATTTCAATTTTAGTTTTGAAAATGCAGTAACTCCACCACTTACTGATATCAAAATATATATATACACTACCACTTATCGGATGAAAATTGAAAATTTAAGGTTAAAATTGAAGATGGTGCCTGAGTAAAAAAATGGATTTTTTTTTTTTTCTTTTCCTCATAATTCTAATATATTTTTTAAATATAATAATTGCTACATAAAAAAGAAAAAAGAAACGTTTTACAAAAATATAAAATGGGTGTATTTGAATTATTTAGGAAAATGAAGCCGAAGAAGAACTGAGAGAAGCTTTCAAAGTGTTTGACATGGATCAAGATGGCTACATATCACCTAGCGAGGTTTGACTTCTAATTAATCTAGTTTATATTTTCTTAAATTAATTACTTCTTTAAAAACATAATTTTCAAATACTATATAAAGTTAAAAAAAAATTAAATCTCGTTTTAATTGCCCAACTTTCCTATTTATTTCATTTTCATCTCTAAGCTTTCAAGTATTTTAATTTAAATCTTCCAACATGTTAATGTCTCAATTTGTTAATTAATTTCTAAATTATGCATAACACGAAAAACTACTTTCACCCTTATGTTGATTTTTTTTCTATTAGTTATTTAACAAAAAAATTTTAAAATAAATTTGAAATCTCACACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAAAGAAGAAGGAAAATTTAAAAATCAAAATAAACAAAAATAAGGGGTTGGTAGCAAAATGAAGATTTGAATTTTAGGTAAAGAAAAATATTAAGTCTTCATTATTAAAAAAAAAAAAAGAAAAGAAGAGGAAAAAAGGTTAGTTATTAATTTTGTCTTCTTTTAAATATTATTTTGTTCTCGGTCCTTTTAAAATGTTCATTTTGATCTTAAATTTTCAGCTGTGGTTCATTTTGATCCTCGTACTTTTAAAATGTTTGTTTTGATCCCAATACTATTAAAATGAATATTTTGATTTCTTAATTTCATTTTTAATGTCATTTTTTATATCACAATTTCAACACACACCATACTCAGTATAATTATTGAAATGTAACCATGAATTTATATTAAAGTGTTTTCGTTATGTGTAAAATTTGTGTTAATATTTACAAAATAGGGAACAAAATAATCATTTTCTTTTAAGTATAGAAACAAAAACAAATAGGATTAAAATAAATCAATATTGAAAGTACATAAACTATTAAAATGAATCAAAACTACAAGTACCTTGACCAAAATAGTATTTTAACCTTGTTTACGATCATTTGGCAGTTGAAGCATGTAATGATCCATTTGGTAGAGAAATTAACAGATGAAGAAGTTGAGCAAATGATCAAAGAAGCTGATTTTGATGGAGATGGATTTGTTAACTATGAAGAATTTGTTAGAATGATGCTTCTTATT

mRNA sequence

TTATTTGGGATCGATGATATTATAGGGTGCATCACGATCAAAGAGTTGGGCACAGCGATTAGATCATTAGATCAAAATCCCACTGAATTAGAACTGCAGACCATGATGAATGAAGTTGATGTCAATGGGAATGGATCCATTGAATTTGGAGAGTTCTTCAATCTCTTGGCAAAAAAGATGAAGGAAAATGAAGCCGAAGAAGAACTGAGAGAAGCTTTCAAAGTGTTTGACATGGATCAAGATGGCTACATATCACCTAGCGAGTTGAAGCATGTAATGATCCATTTGGTAGAGAAATTAACAGATGAAGAAGTTGAGCAAATGATCAAAGAAGCTGATTTTGATGGAGATGGATTTGTTAACTATGAAGAATTTGTTAGAATGATGCTTCTTATT

Coding sequence (CDS)

TTATTTGGGATCGATGATATTATAGGGTGCATCACGATCAAAGAGTTGGGCACAGCGATTAGATCATTAGATCAAAATCCCACTGAATTAGAACTGCAGACCATGATGAATGAAGTTGATGTCAATGGGAATGGATCCATTGAATTTGGAGAGTTCTTCAATCTCTTGGCAAAAAAGATGAAGGAAAATGAAGCCGAAGAAGAACTGAGAGAAGCTTTCAAAGTGTTTGACATGGATCAAGATGGCTACATATCACCTAGCGAGTTGAAGCATGTAATGATCCATTTGGTAGAGAAATTAACAGATGAAGAAGTTGAGCAAATGATCAAAGAAGCTGATTTTGATGGAGATGGATTTGTTAACTATGAAGAATTTGTTAGAATGATGCTTCTTATT

Protein sequence

LFGIDDIIGCITIKELGTAIRSLDQNPTELELQTMMNEVDVNGNGSIEFGEFFNLLAKKMKENEAEEELREAFKVFDMDQDGYISPSELKHVMIHLVEKLTDEEVEQMIKEADFDGDGFVNYEEFVRMMLLI

Homology

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BLAST of MS005299 vs. NCBI nr
Match: XP_022999410.1 (calmodulin-like protein 8 [Cucurbita maxima])

HSP 1 Score: 216.1 bits (549), Expect = 1.8e-52
Identity = 106/124 (85.48%), Postives = 116/124 (93.55%), Query Frame = 0


Query: 9   GCITIKELGTAIRSLDQNPTELELQTMMNEVDVNGNGSIEFGEFFNLLAKKMKENEAEEE 68
           GCITIKEL TAIRSL QNPTE ELQ MMNEVD+NG+GSIEF EFFNL+AKKMKENEAEEE
Sbjct: 22  GCITIKELATAIRSLHQNPTEEELQMMMNEVDINGSGSIEFEEFFNLMAKKMKENEAEEE 81

Query: 69  LREAFKVFDMDQDGYISPSELKHVMIHLVEKLTDEEVEQMIKEADFDGDGFVNYEEFVRM 128
           LREAFKVFDMDQDG+ISP+ELKHVMIH+VEKLTDEEVEQM+KEAD DGDG ++YEEF +M
Sbjct: 82  LREAFKVFDMDQDGFISPNELKHVMIHMVEKLTDEEVEQMVKEADLDGDGLIDYEEFAKM 141

Query: 129 MLLI 133
           MLLI
Sbjct: 142 MLLI 145

[Close]

BLAST of MS005299 vs. NCBI nr
Match: XP_038890963.1 (calmodulin-like protein 8 [Benincasa hispida])

HSP 1 Score: 214.2 bits (544), Expect = 7.0e-52
Identity = 105/124 (84.68%), Postives = 114/124 (91.94%), Query Frame = 0


Query: 9   GCITIKELGTAIRSLDQNPTELELQTMMNEVDVNGNGSIEFGEFFNLLAKKMKENEAEEE 68
           GCITI+EL TAIRSL  NPTE ELQ MMNEVDVNGNGSIEFGEFFNL+AKKMKENEAEEE
Sbjct: 23  GCITIEELATAIRSLHHNPTEEELQMMMNEVDVNGNGSIEFGEFFNLMAKKMKENEAEEE 82

Query: 69  LREAFKVFDMDQDGYISPSELKHVMIHLVEKLTDEEVEQMIKEADFDGDGFVNYEEFVRM 128
           L EAFK+FDMD+DGYISP ELKHVMIH+VEKLTDEEVEQM+ EAD DGDG ++YEEFV+M
Sbjct: 83  LSEAFKLFDMDEDGYISPDELKHVMIHMVEKLTDEEVEQMVNEADLDGDGLIDYEEFVKM 142

Query: 129 MLLI 133
           MLLI
Sbjct: 143 MLLI 146

[Close]

BLAST of MS005299 vs. NCBI nr
Match: KAG7030302.1 (Calmodulin-like protein 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 213.8 bits (543), Expect = 9.1e-52
Identity = 106/124 (85.48%), Postives = 116/124 (93.55%), Query Frame = 0


Query: 9   GCITIKELGTAIRSLDQNPTELELQTMMNEVDVNGNGSIEFGEFFNLLAKKMKENEAEEE 68
           GCITIKEL TAIRSL QNPTE ELQ MMNEVDVNG+GSIEF EFFNL+AKKMKENEAEEE
Sbjct: 22  GCITIKELATAIRSLHQNPTEEELQIMMNEVDVNGSGSIEFEEFFNLMAKKMKENEAEEE 81

Query: 69  LREAFKVFDMDQDGYISPSELKHVMIHLVEKLTDEEVEQMIKEADFDGDGFVNYEEFVRM 128
           LREAFKVFDMDQDG+ISP+ELK+VMIH+VEKLTDEEVEQM+KEAD DGDG ++YEEF +M
Sbjct: 82  LREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDEEVEQMVKEADLDGDGLIDYEEFAKM 141

Query: 129 MLLI 133
           MLLI
Sbjct: 142 MLLI 145

[Close]

BLAST of MS005299 vs. NCBI nr
Match: KAG6599302.1 (Calmodulin-like protein 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 213.0 bits (541), Expect = 1.6e-51
Identity = 105/124 (84.68%), Postives = 116/124 (93.55%), Query Frame = 0


Query: 9   GCITIKELGTAIRSLDQNPTELELQTMMNEVDVNGNGSIEFGEFFNLLAKKMKENEAEEE 68
           GCITIKEL TAIRSL QNPTE ELQ MMNEVDVNG+GSIEF EFFNL+AKKMKENEAEEE
Sbjct: 22  GCITIKELATAIRSLQQNPTEEELQIMMNEVDVNGSGSIEFEEFFNLMAKKMKENEAEEE 81

Query: 69  LREAFKVFDMDQDGYISPSELKHVMIHLVEKLTDEEVEQMIKEADFDGDGFVNYEEFVRM 128
           LREAFKVFDMDQDG+ISP+ELK+VMIH+VEKLTD+EVEQM+KEAD DGDG ++YEEF +M
Sbjct: 82  LREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDDEVEQMVKEADLDGDGLIDYEEFAKM 141

Query: 129 MLLI 133
           MLLI
Sbjct: 142 MLLI 145

[Close]

BLAST of MS005299 vs. NCBI nr
Match: XP_023547072.1 (calmodulin-like protein 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 211.8 bits (538), Expect = 3.5e-51
Identity = 105/124 (84.68%), Postives = 116/124 (93.55%), Query Frame = 0


Query: 9   GCITIKELGTAIRSLDQNPTELELQTMMNEVDVNGNGSIEFGEFFNLLAKKMKENEAEEE 68
           GCITIKEL TAIRSL QNPTE ELQ MMNEVDVNG+GSIEF EFFNL+AKKMKE+EAEEE
Sbjct: 22  GCITIKELATAIRSLHQNPTEEELQIMMNEVDVNGSGSIEFEEFFNLMAKKMKESEAEEE 81

Query: 69  LREAFKVFDMDQDGYISPSELKHVMIHLVEKLTDEEVEQMIKEADFDGDGFVNYEEFVRM 128
           LREAFKVFDMDQDG+ISP+ELK+VMIH+VEKLTDEEVEQM+KEAD DGDG ++YEEF +M
Sbjct: 82  LREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDEEVEQMVKEADLDGDGLIDYEEFAKM 141

Query: 129 MLLI 133
           MLLI
Sbjct: 142 MLLI 145

[Close]

BLAST of MS005299 vs. ExPASy Swiss-Prot
Match: O23320 (Calmodulin-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=CML8 PE=1 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 6.6e-45
Identity = 89/124 (71.77%), Postives = 107/124 (86.29%), Query Frame = 0


Query: 9   GCITIKELGTAIRSLDQNPTELELQTMMNEVDVNGNGSIEFGEFFNLLAKKMKENEAEEE 68
           GCIT++EL T IRSLDQNPTE EL  ++ E+D + NG+IEF EF NL+AKK++E++AEEE
Sbjct: 27  GCITVEELATVIRSLDQNPTEQELHDIITEIDSDSNGTIEFAEFLNLMAKKLQESDAEEE 86

Query: 69  LREAFKVFDMDQDGYISPSELKHVMIHLVEKLTDEEVEQMIKEADFDGDGFVNYEEFVRM 128
           L+EAFKVFD DQ+GYIS SEL HVMI+L EKLTDEEVEQMIKEAD DGDG VNY+EFV+M
Sbjct: 87  LKEAFKVFDKDQNGYISASELSHVMINLGEKLTDEEVEQMIKEADLDGDGQVNYDEFVKM 146

Query: 129 MLLI 133
           M+ I
Sbjct: 147 MINI 150

[Close]

BLAST of MS005299 vs. ExPASy Swiss-Prot
Match: Q9LIK5 (Calmodulin-like protein 11 OS=Arabidopsis thaliana OX=3702 GN=CML11 PE=2 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 8.6e-45
Identity = 88/123 (71.54%), Postives = 106/123 (86.18%), Query Frame = 0


Query: 9   GCITIKELGTAIRSLDQNPTELELQTMMNEVDVNGNGSIEFGEFFNLLAKKMKENEAEEE 68
           GCIT  EL T IRSLDQNPTE ELQ M+ E+D +GNG+IEF EF NL+A +++E +A+EE
Sbjct: 49  GCITADELATVIRSLDQNPTEQELQDMITEIDSDGNGTIEFSEFLNLMANQLQETDADEE 108

Query: 69  LREAFKVFDMDQDGYISPSELKHVMIHLVEKLTDEEVEQMIKEADFDGDGFVNYEEFVRM 128
           L+EAFKVFD DQ+GYIS SEL+HVMI+L EKLTDEEV+QMIKEAD DGDG VNY+EFVRM
Sbjct: 109 LKEAFKVFDKDQNGYISASELRHVMINLGEKLTDEEVDQMIKEADLDGDGQVNYDEFVRM 168

Query: 129 MLL 132
           M++
Sbjct: 169 MMI 171

[Close]

BLAST of MS005299 vs. ExPASy Swiss-Prot
Match: P0DH95 (Calmodulin-1 OS=Arabidopsis thaliana OX=3702 GN=CAM1 PE=1 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 4.3e-44
Identity = 85/122 (69.67%), Postives = 107/122 (87.70%), Query Frame = 0


Query: 9   GCITIKELGTAIRSLDQNPTELELQTMMNEVDVNGNGSIEFGEFFNLLAKKMKENEAEEE 68
           GCIT KELGT +RSL QNPTE ELQ M+NEVD +GNG+I+F EF NL+AKKMK+ ++EEE
Sbjct: 26  GCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMAKKMKDTDSEEE 85

Query: 69  LREAFKVFDMDQDGYISPSELKHVMIHLVEKLTDEEVEQMIKEADFDGDGFVNYEEFVRM 128
           L+EAF+VFD DQ+G+IS +EL+HVM +L EKLTDEEVE+MI+EAD DGDG +NYEEFV++
Sbjct: 86  LKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVEEMIREADVDGDGQINYEEFVKI 145

Query: 129 ML 131
           M+
Sbjct: 146 MM 147

[Close]

BLAST of MS005299 vs. ExPASy Swiss-Prot
Match: P0DH96 (Calmodulin-4 OS=Arabidopsis thaliana OX=3702 GN=CAM4 PE=1 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 4.3e-44
Identity = 85/122 (69.67%), Postives = 107/122 (87.70%), Query Frame = 0


Query: 9   GCITIKELGTAIRSLDQNPTELELQTMMNEVDVNGNGSIEFGEFFNLLAKKMKENEAEEE 68
           GCIT KELGT +RSL QNPTE ELQ M+NEVD +GNG+I+F EF NL+AKKMK+ ++EEE
Sbjct: 26  GCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMAKKMKDTDSEEE 85

Query: 69  LREAFKVFDMDQDGYISPSELKHVMIHLVEKLTDEEVEQMIKEADFDGDGFVNYEEFVRM 128
           L+EAF+VFD DQ+G+IS +EL+HVM +L EKLTDEEVE+MI+EAD DGDG +NYEEFV++
Sbjct: 86  LKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVEEMIREADVDGDGQINYEEFVKI 145

Query: 129 ML 131
           M+
Sbjct: 146 MM 147

[Close]

BLAST of MS005299 vs. ExPASy Swiss-Prot
Match: P04464 (Calmodulin OS=Triticum aestivum OX=4565 PE=1 SV=3)

HSP 1 Score: 178.3 bits (451), Expect = 5.6e-44
Identity = 84/122 (68.85%), Postives = 107/122 (87.70%), Query Frame = 0


Query: 9   GCITIKELGTAIRSLDQNPTELELQTMMNEVDVNGNGSIEFGEFFNLLAKKMKENEAEEE 68
           GCIT KELGT +RSL QNPTE ELQ M+NEVD +GNG+I+F EF NL+A+KMK+ ++EEE
Sbjct: 26  GCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMARKMKDTDSEEE 85

Query: 69  LREAFKVFDMDQDGYISPSELKHVMIHLVEKLTDEEVEQMIKEADFDGDGFVNYEEFVRM 128
           L+EAF+VFD DQDG+IS +EL+HVM +L EKLTDEEV++MI+EAD DGDG +NYEEFV++
Sbjct: 86  LKEAFRVFDKDQDGFISAAELRHVMTNLGEKLTDEEVDEMIREADVDGDGQINYEEFVKV 145

Query: 129 ML 131
           M+
Sbjct: 146 MM 147

[Close]

BLAST of MS005299 vs. ExPASy TrEMBL
Match: A0A6J1KFB0 (calmodulin-like protein 8 OS=Cucurbita maxima OX=3661 GN=LOC111493791 PE=4 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 8.9e-53
Identity = 106/124 (85.48%), Postives = 116/124 (93.55%), Query Frame = 0


Query: 9   GCITIKELGTAIRSLDQNPTELELQTMMNEVDVNGNGSIEFGEFFNLLAKKMKENEAEEE 68
           GCITIKEL TAIRSL QNPTE ELQ MMNEVD+NG+GSIEF EFFNL+AKKMKENEAEEE
Sbjct: 22  GCITIKELATAIRSLHQNPTEEELQMMMNEVDINGSGSIEFEEFFNLMAKKMKENEAEEE 81

Query: 69  LREAFKVFDMDQDGYISPSELKHVMIHLVEKLTDEEVEQMIKEADFDGDGFVNYEEFVRM 128
           LREAFKVFDMDQDG+ISP+ELKHVMIH+VEKLTDEEVEQM+KEAD DGDG ++YEEF +M
Sbjct: 82  LREAFKVFDMDQDGFISPNELKHVMIHMVEKLTDEEVEQMVKEADLDGDGLIDYEEFAKM 141

Query: 129 MLLI 133
           MLLI
Sbjct: 142 MLLI 145

[Close]

BLAST of MS005299 vs. ExPASy TrEMBL
Match: A0A6J1G467 (calmodulin-like protein 8 OS=Cucurbita moschata OX=3662 GN=LOC111450628 PE=4 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.7e-51
Identity = 105/124 (84.68%), Postives = 115/124 (92.74%), Query Frame = 0


Query: 9   GCITIKELGTAIRSLDQNPTELELQTMMNEVDVNGNGSIEFGEFFNLLAKKMKENEAEEE 68
           GCITIKEL TAIRSL QNPTE ELQ MMNEVDVNG+GSIEF EFFNL+ KKMKENEAEEE
Sbjct: 22  GCITIKELATAIRSLHQNPTEEELQIMMNEVDVNGSGSIEFEEFFNLMEKKMKENEAEEE 81

Query: 69  LREAFKVFDMDQDGYISPSELKHVMIHLVEKLTDEEVEQMIKEADFDGDGFVNYEEFVRM 128
           LREAFKVFDMDQDG+ISP+ELK+VMIH+VEKLTDEEVEQM+KEAD DGDG ++YEEF +M
Sbjct: 82  LREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDEEVEQMVKEADLDGDGLIDYEEFAKM 141

Query: 129 MLLI 133
           MLLI
Sbjct: 142 MLLI 145

[Close]

BLAST of MS005299 vs. ExPASy TrEMBL
Match: A0A5D3D4E4 (Calmodulin-like protein 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G001380 PE=4 SV=1)

HSP 1 Score: 208.0 bits (528), Expect = 2.4e-50
Identity = 104/124 (83.87%), Postives = 113/124 (91.13%), Query Frame = 0


Query: 9   GCITIKELGTAIRSLDQNPTELELQTMMNEVDVNGNGSIEFGEFFNLLAKKMKENEAEEE 68
           GCITI+EL TAIRSL  NPTE ELQ MMNEVDVNGNG IEFGEFFNL+AKKMKENEAEEE
Sbjct: 23  GCITIEELATAIRSLHHNPTEEELQIMMNEVDVNGNGYIEFGEFFNLMAKKMKENEAEEE 82

Query: 69  LREAFKVFDMDQDGYISPSELKHVMIHLVEKLTDEEVEQMIKEADFDGDGFVNYEEFVRM 128
           LREAFKVFDMD DG ISP+ELK+VMIH+VEKLTDEE+EQM+ EAD DGDG V+YEEFV+M
Sbjct: 83  LREAFKVFDMDDDGKISPNELKNVMIHMVEKLTDEEIEQMVYEADLDGDGLVDYEEFVKM 142

Query: 129 MLLI 133
           MLLI
Sbjct: 143 MLLI 146

[Close]

BLAST of MS005299 vs. ExPASy TrEMBL
Match: A0A1S3C386 (calmodulin-like protein 8 OS=Cucumis melo OX=3656 GN=LOC103495973 PE=4 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 3.2e-50
Identity = 103/124 (83.06%), Postives = 113/124 (91.13%), Query Frame = 0


Query: 9   GCITIKELGTAIRSLDQNPTELELQTMMNEVDVNGNGSIEFGEFFNLLAKKMKENEAEEE 68
           GCITI+EL TAIRSL  NPTE ELQ MMNEVDVNGNG IEFGEFFNL+AKKMKENEAEEE
Sbjct: 23  GCITIEELATAIRSLHHNPTEEELQIMMNEVDVNGNGYIEFGEFFNLMAKKMKENEAEEE 82

Query: 69  LREAFKVFDMDQDGYISPSELKHVMIHLVEKLTDEEVEQMIKEADFDGDGFVNYEEFVRM 128
           LREAFKVFDMD DG ISP+ELK+VMIH+VEKLTDEE+EQM+ EAD DGDG ++YEEFV+M
Sbjct: 83  LREAFKVFDMDDDGKISPNELKNVMIHMVEKLTDEEIEQMVYEADLDGDGLIDYEEFVKM 142

Query: 129 MLLI 133
           MLLI
Sbjct: 143 MLLI 146

[Close]

BLAST of MS005299 vs. ExPASy TrEMBL
Match: A0A0A0LFI9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G011480 PE=4 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 5.4e-50
Identity = 102/124 (82.26%), Postives = 112/124 (90.32%), Query Frame = 0


Query: 9   GCITIKELGTAIRSLDQNPTELELQTMMNEVDVNGNGSIEFGEFFNLLAKKMKENEAEEE 68
           GCITI EL TAIRSL  NPTE ELQ MMNEVDVNGNG IEFGEFFNL+AKKMKENEAE+E
Sbjct: 23  GCITINELATAIRSLHHNPTEEELQIMMNEVDVNGNGYIEFGEFFNLMAKKMKENEAEDE 82

Query: 69  LREAFKVFDMDQDGYISPSELKHVMIHLVEKLTDEEVEQMIKEADFDGDGFVNYEEFVRM 128
           LREAFKVFDMD DG ISP+ELK+VMIH+VEKLTDEE+EQM+ EAD DGDG ++YEEFV+M
Sbjct: 83  LREAFKVFDMDDDGKISPNELKNVMIHMVEKLTDEEIEQMVNEADLDGDGLIDYEEFVKM 142

Query: 129 MLLI 133
           MLLI
Sbjct: 143 MLLI 146

[Close]

BLAST of MS005299 vs. TAIR 10
Match: AT4G14640.1 (calmodulin 8 )

HSP 1 Score: 181.4 bits (459), Expect = 4.7e-46
Identity = 89/124 (71.77%), Postives = 107/124 (86.29%), Query Frame = 0


Query: 9   GCITIKELGTAIRSLDQNPTELELQTMMNEVDVNGNGSIEFGEFFNLLAKKMKENEAEEE 68
           GCIT++EL T IRSLDQNPTE EL  ++ E+D + NG+IEF EF NL+AKK++E++AEEE
Sbjct: 27  GCITVEELATVIRSLDQNPTEQELHDIITEIDSDSNGTIEFAEFLNLMAKKLQESDAEEE 86

Query: 69  LREAFKVFDMDQDGYISPSELKHVMIHLVEKLTDEEVEQMIKEADFDGDGFVNYEEFVRM 128
           L+EAFKVFD DQ+GYIS SEL HVMI+L EKLTDEEVEQMIKEAD DGDG VNY+EFV+M
Sbjct: 87  LKEAFKVFDKDQNGYISASELSHVMINLGEKLTDEEVEQMIKEADLDGDGQVNYDEFVKM 146

Query: 129 MLLI 133
           M+ I
Sbjct: 147 MINI 150

[Close]

BLAST of MS005299 vs. TAIR 10
Match: AT3G22930.1 (calmodulin-like 11 )

HSP 1 Score: 181.0 bits (458), Expect = 6.1e-46
Identity = 88/123 (71.54%), Postives = 106/123 (86.18%), Query Frame = 0


Query: 9   GCITIKELGTAIRSLDQNPTELELQTMMNEVDVNGNGSIEFGEFFNLLAKKMKENEAEEE 68
           GCIT  EL T IRSLDQNPTE ELQ M+ E+D +GNG+IEF EF NL+A +++E +A+EE
Sbjct: 49  GCITADELATVIRSLDQNPTEQELQDMITEIDSDGNGTIEFSEFLNLMANQLQETDADEE 108

Query: 69  LREAFKVFDMDQDGYISPSELKHVMIHLVEKLTDEEVEQMIKEADFDGDGFVNYEEFVRM 128
           L+EAFKVFD DQ+GYIS SEL+HVMI+L EKLTDEEV+QMIKEAD DGDG VNY+EFVRM
Sbjct: 109 LKEAFKVFDKDQNGYISASELRHVMINLGEKLTDEEVDQMIKEADLDGDGQVNYDEFVRM 168

Query: 129 MLL 132
           M++
Sbjct: 169 MMI 171

[Close]

BLAST of MS005299 vs. TAIR 10
Match: AT2G41110.2 (calmodulin 2 )

HSP 1 Score: 179.9 bits (455), Expect = 1.4e-45
Identity = 85/124 (68.55%), Postives = 109/124 (87.90%), Query Frame = 0


Query: 7   IIGCITIKELGTAIRSLDQNPTELELQTMMNEVDVNGNGSIEFGEFFNLLAKKMKENEAE 66
           I+GCIT KELGT +RSL QNPTE ELQ M+NEVD +GNG+I+F EF NL+A+KMK+ ++E
Sbjct: 36  IVGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMARKMKDTDSE 95

Query: 67  EELREAFKVFDMDQDGYISPSELKHVMIHLVEKLTDEEVEQMIKEADFDGDGFVNYEEFV 126
           EEL+EAF+VFD DQ+G+IS +EL+HVM +L EKLTDEEV++MIKEAD DGDG +NYEEFV
Sbjct: 96  EELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDEMIKEADVDGDGQINYEEFV 155

Query: 127 RMML 131
           ++M+
Sbjct: 156 KVMM 159

[Close]

BLAST of MS005299 vs. TAIR 10
Match: AT1G66410.2 (calmodulin 4 )

HSP 1 Score: 179.5 bits (454), Expect = 1.8e-45
Identity = 86/125 (68.80%), Postives = 108/125 (86.40%), Query Frame = 0


Query: 6   DIIGCITIKELGTAIRSLDQNPTELELQTMMNEVDVNGNGSIEFGEFFNLLAKKMKENEA 65
           D  GCIT KELGT +RSL QNPTE ELQ M+NEVD +GNG+I+F EF NL+AKKMK+ ++
Sbjct: 33  DSCGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMAKKMKDTDS 92

Query: 66  EEELREAFKVFDMDQDGYISPSELKHVMIHLVEKLTDEEVEQMIKEADFDGDGFVNYEEF 125
           EEEL+EAF+VFD DQ+G+IS +EL+HVM +L EKLTDEEVE+MI+EAD DGDG +NYEEF
Sbjct: 93  EEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVEEMIREADVDGDGQINYEEF 152

Query: 126 VRMML 131
           V++M+
Sbjct: 153 VKIMM 157

[Close]

BLAST of MS005299 vs. TAIR 10
Match: AT1G66410.1 (calmodulin 4 )

HSP 1 Score: 178.7 bits (452), Expect = 3.0e-45
Identity = 85/122 (69.67%), Postives = 107/122 (87.70%), Query Frame = 0


Query: 9   GCITIKELGTAIRSLDQNPTELELQTMMNEVDVNGNGSIEFGEFFNLLAKKMKENEAEEE 68
           GCIT KELGT +RSL QNPTE ELQ M+NEVD +GNG+I+F EF NL+AKKMK+ ++EEE
Sbjct: 26  GCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMAKKMKDTDSEEE 85

Query: 69  LREAFKVFDMDQDGYISPSELKHVMIHLVEKLTDEEVEQMIKEADFDGDGFVNYEEFVRM 128
           L+EAF+VFD DQ+G+IS +EL+HVM +L EKLTDEEVE+MI+EAD DGDG +NYEEFV++
Sbjct: 86  LKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVEEMIREADVDGDGQINYEEFVKI 145

Query: 129 ML 131
           M+
Sbjct: 146 MM 147

The following BLAST results are available for this feature:

BLAST of MS005299 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022999410.1	1.8e-52	85.48	calmodulin-like protein 8 [Cucurbita maxima]	[more]
XP_038890963.1	7.0e-52	84.68	calmodulin-like protein 8 [Benincasa hispida]	[more]
KAG7030302.1	9.1e-52	85.48	Calmodulin-like protein 8, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6599302.1	1.6e-51	84.68	Calmodulin-like protein 8, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023547072.1	3.5e-51	84.68	calmodulin-like protein 8 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS005299 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23320	6.6e-45	71.77	Calmodulin-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=CML8 PE=1 SV=1	[more]
Q9LIK5	8.6e-45	71.54	Calmodulin-like protein 11 OS=Arabidopsis thaliana OX=3702 GN=CML11 PE=2 SV=1	[more]
P0DH95	4.3e-44	69.67	Calmodulin-1 OS=Arabidopsis thaliana OX=3702 GN=CAM1 PE=1 SV=1	[more]
P0DH96	4.3e-44	69.67	Calmodulin-4 OS=Arabidopsis thaliana OX=3702 GN=CAM4 PE=1 SV=1	[more]
P04464	5.6e-44	68.85	Calmodulin OS=Triticum aestivum OX=4565 PE=1 SV=3	[more]

BLAST of MS005299 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KFB0	8.9e-53	85.48	calmodulin-like protein 8 OS=Cucurbita maxima OX=3661 GN=LOC111493791 PE=4 SV=1	[more]
A0A6J1G467	1.7e-51	84.68	calmodulin-like protein 8 OS=Cucurbita moschata OX=3662 GN=LOC111450628 PE=4 SV=...	[more]
A0A5D3D4E4	2.4e-50	83.87	Calmodulin-like protein 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3C386	3.2e-50	83.06	calmodulin-like protein 8 OS=Cucumis melo OX=3656 GN=LOC103495973 PE=4 SV=1	[more]
A0A0A0LFI9	5.4e-50	82.26	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G011480 PE=4 SV=1	[more]

BLAST of MS005299 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G14640.1	4.7e-46	71.77	calmodulin 8	[more]
AT3G22930.1	6.1e-46	71.54	calmodulin-like 11	[more]
AT2G41110.2	1.4e-45	68.55	calmodulin 2	[more]
AT1G66410.2	1.8e-45	68.80	calmodulin 4	[more]
AT1G66410.1	3.0e-45	69.67	calmodulin 4	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 104..132 e-value: 1.1E-6 score: 38.2 coord: 68..96 e-value: 4.3E-7 score: 39.6 coord: 31..59 e-value: 4.5E-5 score: 32.9
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 66..129 e-value: 1.1E-16 score: 61.0
IPR002048	EF-hand domain	PFAM	PF13833	EF-hand_8	coord: 9..58 e-value: 1.8E-6 score: 27.6
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 27..62 score: 12.198088
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 100..132 score: 15.071314
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 64..99 score: 15.936071
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 72..129 e-value: 3.34005E-20 score: 76.0473
None	No IPR available	GENE3D	1.10.238.10		coord: 6..58 e-value: 1.8E-16 score: 62.0
None	No IPR available	GENE3D	1.10.238.10		coord: 59..105 e-value: 3.0E-21 score: 77.1
None	No IPR available	GENE3D	1.10.238.10		coord: 106..131 e-value: 5.3E-6 score: 28.4
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 9..131
None	No IPR available	PANTHER	PTHR23050:SF378	CALMODULIN	coord: 9..131
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 40..52
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 113..125
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 77..89
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 9..130

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS005299.1	MS005299.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding