MS005262 (gene) Bitter gourd (TR) v1

Overview
NameMS005262
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Locationscaffold83: 384341 .. 387806 (+)
RNA-Seq ExpressionMS005262
SyntenyMS005262
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGTGTCCAAAGGGAAATACGGTAAGATTAACTTTCACGGCAGGAAATGGTCCACATTAGAGCTCTCCAAGATCATCATGGCTCTCGTTTTGGCCCTCGGCTTCTCCATGCTTCTCGCTCTCCGGTTCTTCTCTCCTCCCGAAAGTTCTGATCCCAACCTACCCGACCGTCTCGCTTCCGTCAGGCGTAAAGCCGTTGAGAGGTACTCCCGATTCGGCAATGTTTAGTGATTTGATTTCTTCAGCTTGTTATTGGCAGTATCGATTAATTTCGATTTTGATTTGTGGTCTTGTTGAAGTGAGGGGTTGGGGAAGAGAGGGGAGCAGTGGGTTGAGGTCATTTCTTGGGAGCCAAGGGCTTTCATTTATCACAATTTCTTGGTATGTTCTAGAGTAATTATTATTATTATTATTATTTTATTGTAATTTGGTTTAAATGTTTACTGATGTTAGAATGCTTTGAACAGTCCAAGGAAGAGTGCTTGTACTTAATTAGTCTTGCAAAGCCTCGTATGGTGAAGTCAACTGTGATTGATTCCGAAACTGGCAAGAGTATGGATAGCAGGTTTGTATTTCTCATTCTTACATACCAGTAGAGTTTGTCAATTATGACTACGAACAAAGATAGAGGTTAGTTTTGAAATAATTTCATCTTCTAAAGATGATTTATCTATAAATAGTCTTTCTGATCAATGGTCTCTCTAAAAGTAGATTTATTTGAGGGAGGTAAATCCCGAAAGTGGCTGCATATAGTAGATTCAGCTGAGATTTGGTCGGTTTGAAGTATATTTATAGTAAGCTCGGTTTTTCAAAGCCTAAAACTGAAAATAATAAATTGGTTTGAGTTAGGCAGCTGGTTTAGAAAATTGATTTGGTCAAAACCCGGCCACATACGCCTTAGGTGAAAAAAATGGTTATGGAGAAAGCATAAAAGAAGGGTATAACAATACATTTACAAATAAACAAGGGAAACTTTAGTTGATTTGCTACATAATATAATGTTCGCAGGGTGCGCACCAGCTCTGGTATGTTTCTGAGTAGAGGGCAGGACAGAATCATCAGGAACATAGAGAAAAGAATTGCAGACTTTACATTCATTCCTATAGGTAAAGAATTCTAAAGATTACTTATTTCTTTTTCCAATTTCTCATGCATTTATTATCACTCTGTTCTGTAACCTGTGACATATACAAGTATTTAATTGAAACTAGCATTTCTACCAGATGGGCCCGCTGTGCTATGTTACACCAAATGTTTAATTTGTTCATCCTATTGTTATAATTCCTACCTATTTGCTCACAAAGGGCAGTCCAGTTGGCAAGGACTTGTGGTCTCTTAGTCATATTGGCTTAGAGGCTTCAGGTTCGGATCTTCTAGTGAGTTTAATACCAAAAACCCTTGATGTCTATTGGGTCCGGACTTTAGGGCGGTCACTGCCTAGGTACAGGGGAGTAAAGCTCCGGCTCTCAGTTATAAAAAAAAGAAAAGGAATAAATAAATAATTCCTACCTATTTGTCTGGTAATAGTGGTTAGAACAAGTTTTTGTAATTTTTTAAAGAGTTATTTTTTATGTGTTGACTTATCTTTTGGAACAAGTTGTCCTTCTGACTTTGTCCTTATGTAATATGTGGATGGAGAGTGATTTTGATGTATTCAGCACCATCAATCTTTACAAGTTATTTCCTTGTAGATTTGACAACTGAAACATAATTCCCACAAGAAGTTTGGTACTGATCAAGCCAAACATCGAATTTGCTTAAATTATGGCAATTAGTCTCTTACCCCAAGGGTAAACGTAAAGGTTTGAAATGTTTGGAGATATTCTCTAAGGTCTTGGATTTAAGCCTCAATTGAAAACTTAATAACTTAAAATTTTTTTGTTGTCTTTCGGGGTTGGGTTTGAGGTGGGTGAAACCCTCTTAGAGATTTGTAGGTTGCCTCCAAGACCTACTTATAAAAGAAAAGGAACTGATCTGTTTGAAACATATGCATCAGTGCAACCAAAAATTTAAACTTTAAGCTTACTAATCCAAAAACTTAGCTAAGTTTCATGTGATATTGTCTCCATTTCTGTTTTGAGACCCCAAACCAAACCGTGCTTTGATTCATTTACTTGTTTTGATGCTTTCTTGATGTCACTGACATCCTTGGTCTTAATATATTTTTATTCCAAGCAGAGCATGGAGAAGGACTTCAAATTCTGCACTATGAAGTTGGGCAGAAGTATGATGCTCACCATGATTACTTTGTTGATGAGTACAACATCAAAAAAGGAAGCCAAAGAATGGCCACCCTTCTCATGTATTTGTAAGATTCTACTTGATATCTCTCTAATTCATCTGACTCTCTTTCTATAATTATCTCTCTAAATTGCTACCAATACGAATGCAGGTCAGATGTTGAAGAAGGGGGCGAGACGGTGTTCCCGGCTGCAAAAGGAAATTTCAGCTCTGTGCCATGGTGGAATGAGCTTTCTGAATGTGGTAAAGGTGGACTTTCTGTAAAACCAAAGATGGGGGATGCGTTATTGTTCTGGAGCATGAAGCCTGATGCTTCCTTAGATCCTACAAGTTTGCATGGTTAGTGATATTTACTGTCATCAAAATCTTGAATATTCCCCTCTGTTTTCAAATTTATCGTTTGAGAATTATATATAATCTAAAAATTTCTTCTTTAGGCCACACAACAGATTTGATTTCTGAGCGAATTCAAGAAAAGTTTTTGAAAACAATTATTTTAGTTTTCCAAATTTGGATTGGATTTTGAAAATGTTTTGAAAAAGTAGATATTACCAGAAAAGGTAATACATATGTGTAATACATATGTGGAAGTAATGTTTAATTATAAGCTTACTTAAAAAAAAAAGATTATTAGATGAGACTATTTCAAAATCTTACTTGCAAAAAAGAAAAATATTTGTATCGATCATGGGACTGTCAATAAGGGCCAATGTAAATAGCAAAGGGCTTAGAGGGAATGAGTTCAAACCATGGTGGCCACTACCTGCAATTTAGTATCGTACGAATTACCTTGTCAACCGAATGTAGTAGGGTCAGGCAGTTGGCATGTGATTAGTTGAGGTGCGCGCAAGCTGGTCCGAACATACAGATATAAAAAATATATGTATATATTTGTAATAGTAGAAAATTAAAAACAAGCATTTTTGGGGCGGGATGGACAGTATAACATAAATGAAGTTTAAATTATATGTTGGCCCTGTAACTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGATCAAACCATCAACTTTTGGATTCAATTTTGATTGTTTTATACATGCCTTTGCTCAAGAGTTGGGCACATTTTAATAATTATTAAGGAGAACTAATTACATTTTTGTGATAGGTGCTTGCCCTGTAATAAAAGGGAACAAATGGTCATGTACAAAGTGGATGCATGTTAACAAATACTCG

mRNA sequence

ATGGCGGTGTCCAAAGGGAAATACGGTAAGATTAACTTTCACGGCAGGAAATGGTCCACATTAGAGCTCTCCAAGATCATCATGGCTCTCGTTTTGGCCCTCGGCTTCTCCATGCTTCTCGCTCTCCGGTTCTTCTCTCCTCCCGAAAGTTCTGATCCCAACCTACCCGACCGTCTCGCTTCCGTCAGGCGTAAAGCCGTTGAGAGTGAGGGGTTGGGGAAGAGAGGGGAGCAGTGGGTTGAGGTCATTTCTTGGGAGCCAAGGGCTTTCATTTATCACAATTTCTTGTCCAAGGAAGAGTGCTTGTACTTAATTAGTCTTGCAAAGCCTCGTATGGTGAAGTCAACTGTGATTGATTCCGAAACTGGCAAGAGTATGGATAGCAGGGTGCGCACCAGCTCTGGTATGTTTCTGAGTAGAGGGCAGGACAGAATCATCAGGAACATAGAGAAAAGAATTGCAGACTTTACATTCATTCCTATAGCAGAGCATGGAGAAGGACTTCAAATTCTGCACTATGAAGTTGGGCAGAAGTATGATGCTCACCATGATTACTTTGTTGATGAGTACAACATCAAAAAAGGAAGCCAAAGAATGGCCACCCTTCTCATGTATTTGTCAGATGTTGAAGAAGGGGGCGAGACGGTGTTCCCGGCTGCAAAAGGAAATTTCAGCTCTGTGCCATGGTGGAATGAGCTTTCTGAATGTGGTAAAGGTGGACTTTCTGTAAAACCAAAGATGGGGGATGCGTTATTGTTCTGGAGCATGAAGCCTGATGCTTCCTTAGATCCTACAAGTTTGCATGGTGCTTGCCCTGTAATAAAAGGGAACAAATGGTCATGTACAAAGTGGATGCATGTTAACAAATACTCG

Coding sequence (CDS)

ATGGCGGTGTCCAAAGGGAAATACGGTAAGATTAACTTTCACGGCAGGAAATGGTCCACATTAGAGCTCTCCAAGATCATCATGGCTCTCGTTTTGGCCCTCGGCTTCTCCATGCTTCTCGCTCTCCGGTTCTTCTCTCCTCCCGAAAGTTCTGATCCCAACCTACCCGACCGTCTCGCTTCCGTCAGGCGTAAAGCCGTTGAGAGTGAGGGGTTGGGGAAGAGAGGGGAGCAGTGGGTTGAGGTCATTTCTTGGGAGCCAAGGGCTTTCATTTATCACAATTTCTTGTCCAAGGAAGAGTGCTTGTACTTAATTAGTCTTGCAAAGCCTCGTATGGTGAAGTCAACTGTGATTGATTCCGAAACTGGCAAGAGTATGGATAGCAGGGTGCGCACCAGCTCTGGTATGTTTCTGAGTAGAGGGCAGGACAGAATCATCAGGAACATAGAGAAAAGAATTGCAGACTTTACATTCATTCCTATAGCAGAGCATGGAGAAGGACTTCAAATTCTGCACTATGAAGTTGGGCAGAAGTATGATGCTCACCATGATTACTTTGTTGATGAGTACAACATCAAAAAAGGAAGCCAAAGAATGGCCACCCTTCTCATGTATTTGTCAGATGTTGAAGAAGGGGGCGAGACGGTGTTCCCGGCTGCAAAAGGAAATTTCAGCTCTGTGCCATGGTGGAATGAGCTTTCTGAATGTGGTAAAGGTGGACTTTCTGTAAAACCAAAGATGGGGGATGCGTTATTGTTCTGGAGCATGAAGCCTGATGCTTCCTTAGATCCTACAAGTTTGCATGGTGCTTGCCCTGTAATAAAAGGGAACAAATGGTCATGTACAAAGTGGATGCATGTTAACAAATACTCG

Protein sequence

MAVSKGKYGKINFHGRKWSTLELSKIIMALVLALGFSMLLALRFFSPPESSDPNLPDRLASVRRKAVESEGLGKRGEQWVEVISWEPRAFIYHNFLSKEECLYLISLAKPRMVKSTVIDSETGKSMDSRVRTSSGMFLSRGQDRIIRNIEKRIADFTFIPIAEHGEGLQILHYEVGQKYDAHHDYFVDEYNIKKGSQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKMGDALLFWSMKPDASLDPTSLHGACPVIKGNKWSCTKWMHVNKYS
Homology
[Close]
BLAST of MS005262 vs. NCBI nr
Match: XP_022143254.1 (probable prolyl 4-hydroxylase 3 [Momordica charantia])

HSP 1 Score: 593.6 bits (1529), Expect = 9.3e-166
Identity = 290/291 (99.66%), Postives = 290/291 (99.66%), Query Frame = 0 

Query: 1   MAVSKGKYGKINFHGRKWSTLELSKIIMALVLALGFSMLLALRFFSPPESSDPNLPDRLA 60
           MAVSKGKYGKINFHGRKWSTLELSKIIMALVLALGFSMLLALRFFSPPESSDPNLPDRLA
Sbjct: 1   MAVSKGKYGKINFHGRKWSTLELSKIIMALVLALGFSMLLALRFFSPPESSDPNLPDRLA 60

Query: 61  SVRRKAVESEGLGKRGEQWVEVISWEPRAFIYHNFLSKEECLYLISLAKPRMVKSTVIDS 120
           SVRRKAVESEGLGKRGEQWVEVISWEPRAFIYHNFLSKEECLYLISLAKPRMVKSTVIDS
Sbjct: 61  SVRRKAVESEGLGKRGEQWVEVISWEPRAFIYHNFLSKEECLYLISLAKPRMVKSTVIDS 120

Query: 121 ETGKSMDSRVRTSSGMFLSRGQDRIIRNIEKRIADFTFIPIAEHGEGLQILHYEVGQKYD 180
           ETGKSMDSRVRTSSGMFLSRGQDRIIRNIEKRIADFTFIPI EHGEGLQILHYEVGQKYD
Sbjct: 121 ETGKSMDSRVRTSSGMFLSRGQDRIIRNIEKRIADFTFIPI-EHGEGLQILHYEVGQKYD 180

Query: 181 AHHDYFVDEYNIKKGSQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGG 240
           AHHDYFVDEYNIKKGSQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGG
Sbjct: 181 AHHDYFVDEYNIKKGSQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGG 240

Query: 241 LSVKPKMGDALLFWSMKPDASLDPTSLHGACPVIKGNKWSCTKWMHVNKYS 292
           LSVKPKMGDALLFWSMKPDASLDPTSLHGACPVIKGNKWSCTKWMHVNKYS
Sbjct: 241 LSVKPKMGDALLFWSMKPDASLDPTSLHGACPVIKGNKWSCTKWMHVNKYS 290

[Close]
BLAST of MS005262 vs. NCBI nr
Match: XP_038889689.1 (probable prolyl 4-hydroxylase 3 [Benincasa hispida])

HSP 1 Score: 528.5 bits (1360), Expect = 3.7e-146
Identity = 256/290 (88.28%), Postives = 272/290 (93.79%), Query Frame = 0 

Query: 1   MAVSKGKYGKINFHGRKWSTLELSKIIMALVLALGFSMLLALRFFSPPESSDPNLPDRLA 60
           MA+SKGKY KI   G+KWST ELSK+IMALVLALGF MLLALRFFSPPE+S  NLP  LA
Sbjct: 1   MALSKGKYTKI--QGKKWSTFELSKMIMALVLALGFFMLLALRFFSPPETSHRNLPHHLA 60

Query: 61  SVRRKAVESEGLGKRGEQWVEVISWEPRAFIYHNFLSKEECLYLISLAKPRMVKSTVIDS 120
           SVR  AVES+GLGKRG+QWVE ISWEPRAF+YHNFLSKEECLYLISLAKP M KSTV+DS
Sbjct: 61  SVRHSAVESDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDS 120

Query: 121 ETGKSMDSRVRTSSGMFLSRGQDRIIRNIEKRIADFTFIPIAEHGEGLQILHYEVGQKYD 180
           +TGKS+DSRVRTSSGMFL+RGQD+II NIEKRIADFTFIPI EHGEGLQILHYEVGQKYD
Sbjct: 121 KTGKSVDSRVRTSSGMFLNRGQDKIISNIEKRIADFTFIPI-EHGEGLQILHYEVGQKYD 180

Query: 181 AHHDYFVDEYNIKKGSQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGG 240
           AH+DYFVDEYNIKKG QRMATLLMYLSDVEEGGETVFPAA+GNFSSVPWWNELSECGKGG
Sbjct: 181 AHYDYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAARGNFSSVPWWNELSECGKGG 240

Query: 241 LSVKPKMGDALLFWSMKPDASLDPTSLHGACPVIKGNKWSCTKWMHVNKY 291
           LSVKPKMGDALLFWSMKPDA+LDPTSLHGACPVI+GNKWSCTKWMHVNKY
Sbjct: 241 LSVKPKMGDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVNKY 287

[Close]
BLAST of MS005262 vs. NCBI nr
Match: XP_008456388.1 (PREDICTED: probable prolyl 4-hydroxylase 3 isoform X2 [Cucumis melo])

HSP 1 Score: 515.4 bits (1326), Expect = 3.2e-142
Identity = 251/290 (86.55%), Postives = 268/290 (92.41%), Query Frame = 0 

Query: 1   MAVSKGKYGKINFHGRKWSTLELSKIIMALVLALGFSMLLALRFFSPPESSDPNLPDRLA 60
           MAVSKGKY  I   G+KWST +LSK+IMALVLALGF ML+ALRFFSPPE+S   LP    
Sbjct: 1   MAVSKGKY--IKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRLP---- 60

Query: 61  SVRRKAVESEGLGKRGEQWVEVISWEPRAFIYHNFLSKEECLYLISLAKPRMVKSTVIDS 120
           SVRR A +S+GLGKRG+QWVE ISWEPRAF+YHNFLSKEECLYLISLAKP M KSTV+DS
Sbjct: 61  SVRRTAFQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDS 120

Query: 121 ETGKSMDSRVRTSSGMFLSRGQDRIIRNIEKRIADFTFIPIAEHGEGLQILHYEVGQKYD 180
           +TGKS+DSRVRTSSGMFL+RGQD+II NIEKRIADFTFIPI EHGEGLQILHYEVGQKYD
Sbjct: 121 KTGKSVDSRVRTSSGMFLNRGQDKIISNIEKRIADFTFIPI-EHGEGLQILHYEVGQKYD 180

Query: 181 AHHDYFVDEYNIKKGSQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGG 240
           AH+DYFVDEYNIKKG QRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGG
Sbjct: 181 AHYDYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGG 240

Query: 241 LSVKPKMGDALLFWSMKPDASLDPTSLHGACPVIKGNKWSCTKWMHVNKY 291
           LSVKPKMGDALLFWSMKPDA+LDPTSLHGACPVI+GNKWSCTKWMHVNKY
Sbjct: 241 LSVKPKMGDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVNKY 283

[Close]
BLAST of MS005262 vs. NCBI nr
Match: XP_011648735.2 (probable prolyl 4-hydroxylase 3 [Cucumis sativus] >KAE8651549.1 hypothetical protein Csa_019368 [Cucumis sativus])

HSP 1 Score: 509.6 bits (1311), Expect = 1.8e-140
Identity = 248/290 (85.52%), Postives = 267/290 (92.07%), Query Frame = 0 

Query: 1   MAVSKGKYGKINFHGRKWSTLELSKIIMALVLALGFSMLLALRFFSPPESSDPNLPDRLA 60
           MA+SKGKY  I   GRKWST +LSK+IMALVLALGF ML+ALRFFSPPE+S      R +
Sbjct: 1   MAISKGKY--IKLQGRKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSH----HRFS 60

Query: 61  SVRRKAVESEGLGKRGEQWVEVISWEPRAFIYHNFLSKEECLYLISLAKPRMVKSTVIDS 120
           SVR  A  S+GLGKRG+QWVE ISWEPRAF+YHNFLSKEECLYLISLAKP M KSTV+DS
Sbjct: 61  SVRHTAFLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDS 120

Query: 121 ETGKSMDSRVRTSSGMFLSRGQDRIIRNIEKRIADFTFIPIAEHGEGLQILHYEVGQKYD 180
           +TG+S+DSRVRTSSGMFL+RGQD+IIRNIEKRIADFTFIPI EHGEGLQILHYEVGQKYD
Sbjct: 121 KTGESVDSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPI-EHGEGLQILHYEVGQKYD 180

Query: 181 AHHDYFVDEYNIKKGSQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGG 240
           AH+DYFVDEYNIKKG QRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGG
Sbjct: 181 AHYDYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGG 240

Query: 241 LSVKPKMGDALLFWSMKPDASLDPTSLHGACPVIKGNKWSCTKWMHVNKY 291
           LSVKPKMGDALLFWSMKPDA+LDPTSLHGACPVI+GNKWSCTKWMHV+KY
Sbjct: 241 LSVKPKMGDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKY 283

[Close]
BLAST of MS005262 vs. NCBI nr
Match: XP_022937931.1 (probable prolyl 4-hydroxylase 3 [Cucurbita moschata])

HSP 1 Score: 496.5 bits (1277), Expect = 1.6e-136
Identity = 237/290 (81.72%), Postives = 263/290 (90.69%), Query Frame = 0 

Query: 1   MAVSKGKYGKINFHGRKWSTLELSKIIMALVLALGFSMLLALRFFSPPESSDPNLPDRLA 60
           MAVSKGKY  + F  RKWST +LSKIIMA +LALG SML+A RFFSPPESS  NL  R+A
Sbjct: 1   MAVSKGKY--VKFQARKWSTFKLSKIIMAFLLALGVSMLIAFRFFSPPESSHSNLLHRVA 60

Query: 61  SVRRKAVESEGLGKRGEQWVEVISWEPRAFIYHNFLSKEECLYLISLAKPRMVKSTVIDS 120
           SV+ +AV S+GLGKRG+QWVE ISWEPRAF+YHNFLSKEECLYLISLAKP M KSTV+D+
Sbjct: 61  SVQHRAVHSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMAKSTVVDN 120

Query: 121 ETGKSMDSRVRTSSGMFLSRGQDRIIRNIEKRIADFTFIPIAEHGEGLQILHYEVGQKYD 180
           +TGKS+DSRVRTSSGMFL RGQ++I+ NIEKRIADFTFIP+ EHGE LQILHYEVGQKYD
Sbjct: 121 KTGKSIDSRVRTSSGMFLKRGQNKIVSNIEKRIADFTFIPV-EHGEALQILHYEVGQKYD 180

Query: 181 AHHDYFVDEYNIKKGSQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGG 240
           AHHDYF DE+NIK+G QRMATLLMYLSDVEEGGETVFPAA+GNFSS+P WNELSECGKGG
Sbjct: 181 AHHDYFADEFNIKQGGQRMATLLMYLSDVEEGGETVFPAAEGNFSSLPGWNELSECGKGG 240

Query: 241 LSVKPKMGDALLFWSMKPDASLDPTSLHGACPVIKGNKWSCTKWMHVNKY 291
           LSVKPKMGDALLFWSMKPD ++DPTSLHGACPVI+GNKWSCTKWM VN+Y
Sbjct: 241 LSVKPKMGDALLFWSMKPDNTVDPTSLHGACPVIRGNKWSCTKWMRVNEY 287

[Close]
BLAST of MS005262 vs. ExPASy Swiss-Prot
Match: Q9LN20 (Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 5.8e-118
Identity = 207/279 (74.19%), Postives = 234/279 (83.87%), Query Frame = 0 

Query: 13  FHGRKWSTLELSKIIMALVLALGFSMLLALRFFSPPESSDPNLPDRLASVRRKAVE-SEG 72
           F  RKWSTL L  + M  +L +   MLLA   FS P ++D + P  L+  RR A E SEG
Sbjct: 9   FQARKWSTLML-VLFMLFMLTIVLLMLLAFGVFSLPINNDESSPIDLSYFRRAATERSEG 68

Query: 73  LGKRGEQWVEVISWEPRAFIYHNFLSKEECLYLISLAKPRMVKSTVIDSETGKSMDSRVR 132
           LGKRG+QW EV+SWEPRAF+YHNFLSKEEC YLISLAKP MVKSTV+DSETGKS DSRVR
Sbjct: 69  LGKRGDQWTEVLSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSETGKSKDSRVR 128

Query: 133 TSSGMFLSRGQDRIIRNIEKRIADFTFIPIAEHGEGLQILHYEVGQKYDAHHDYFVDEYN 192
           TSSG FL RG+D+II+ IEKRIAD+TFIP A+HGEGLQ+LHYE GQKY+ H+DYFVDE+N
Sbjct: 129 TSSGTFLRRGRDKIIKTIEKRIADYTFIP-ADHGEGLQVLHYEAGQKYEPHYDYFVDEFN 188

Query: 193 IKKGSQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKMGDAL 252
            K G QRMAT+LMYLSDVEEGGETVFPAA  NFSSVPW+NELSECGK GLSVKP+MGDAL
Sbjct: 189 TKNGGQRMATMLMYLSDVEEGGETVFPAANMNFSSVPWYNELSECGKKGLSVKPRMGDAL 248

Query: 253 LFWSMKPDASLDPTSLHGACPVIKGNKWSCTKWMHVNKY 291
           LFWSM+PDA+LDPTSLHG CPVI+GNKWS TKWMHV +Y
Sbjct: 249 LFWSMRPDATLDPTSLHGGCPVIRGNKWSSTKWMHVGEY 285

[Close]
BLAST of MS005262 vs. ExPASy Swiss-Prot
Match: F4JZ24 (Probable prolyl 4-hydroxylase 10 OS=Arabidopsis thaliana OX=3702 GN=P4H10 PE=3 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 4.3e-105
Identity = 184/274 (67.15%), Postives = 223/274 (81.39%), Query Frame = 0 

Query: 19  STLELSKIIMALVLALGFSMLLALRFFS-PPESSDPNLPDRLASVRRKAVESEGL-GKRG 78
           STL  + +IM+  + L   +LLA    S P  ++  +  + L S+ RK ++  G    + 
Sbjct: 18  STLVFAVLIMSTFVIL---ILLAFGILSVPSNNAGSSKANDLTSIVRKTLQRSGEDDSKN 77

Query: 79  EQWVEVISWEPRAFIYHNFLSKEECLYLISLAKPRMVKSTVIDSETGKSMDSRVRTSSGM 138
           E+WVE+ISWEPRA +YHNFL+KEEC YLI LAKP M KSTV+D +TGKS DSRVRTSSG 
Sbjct: 78  ERWVEIISWEPRASVYHNFLTKEECKYLIELAKPHMEKSTVVDEKTGKSTDSRVRTSSGT 137

Query: 139 FLSRGQDRIIRNIEKRIADFTFIPIAEHGEGLQILHYEVGQKYDAHHDYFVDEYNIKKGS 198
           FL+RG+D+ IR IEKRI+DFTFIP+ EHGEGLQ+LHYE+GQKY+ H+DYF+DEYN + G 
Sbjct: 138 FLARGRDKTIREIEKRISDFTFIPV-EHGEGLQVLHYEIGQKYEPHYDYFMDEYNTRNGG 197

Query: 199 QRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKMGDALLFWSM 258
           QR+AT+LMYLSDVEEGGETVFPAAKGN+S+VPWWNELSECGKGGLSVKPKMGDALLFWSM
Sbjct: 198 QRIATVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGGLSVKPKMGDALLFWSM 257

Query: 259 KPDASLDPTSLHGACPVIKGNKWSCTKWMHVNKY 291
            PDA+LDP+SLHG C VIKGNKWS TKW+ V++Y
Sbjct: 258 TPDATLDPSSLHGGCAVIKGNKWSSTKWLRVHEY 287

[Close]
BLAST of MS005262 vs. ExPASy Swiss-Prot
Match: F4JNU8 (Probable prolyl 4-hydroxylase 8 OS=Arabidopsis thaliana OX=3702 GN=P4H8 PE=3 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 1.1e-100
Identity = 176/281 (62.63%), Postives = 220/281 (78.29%), Query Frame = 0 

Query: 16  RKWSTLELSKIIMALVLALGFSMLLALRFFS-PPESSDPNLPDRLASVRRKAVESEGLGK 75
           + +ST   + +++ L + L   +L+ L  FS P  +   ++P  L ++ +   E E  G 
Sbjct: 14  KSFSTQTFTVVVLVLFVIL---ILVGLGIFSLPSTNKTSSMPMDLTTIVQTIQERESFGD 73

Query: 76  ----RGEQWVEVISWEPRAFIYHNFLSKEECLYLISLAKPRMVKSTVIDSETGKSMDSRV 135
                G++W+EVISWEPRAF+YHNFL+ EEC +LISLAKP M+KS V+D +TGKS+DSRV
Sbjct: 74  EEDGNGDRWLEVISWEPRAFVYHNFLTNEECEHLISLAKPSMMKSKVVDVKTGKSIDSRV 133

Query: 136 RTSSGMFLSRGQDRIIRNIEKRIADFTFIPIAEHGEGLQILHYEVGQKYDAHHDYFVDEY 195
           RTSSG FL+RG D I+  IE RI+DFTFIP  E+GEGLQ+LHYEVGQ+Y+ HHDYF DE+
Sbjct: 134 RTSSGTFLNRGHDEIVEEIENRISDFTFIP-PENGEGLQVLHYEVGQRYEPHHDYFFDEF 193

Query: 196 NIKKGSQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKMGDA 255
           N++KG QR+AT+LMYLSDV+EGGETVFPAAKGN S VPWW+ELS+CGK GLSV PK  DA
Sbjct: 194 NVRKGGQRIATVLMYLSDVDEGGETVFPAAKGNVSDVPWWDELSQCGKEGLSVLPKKRDA 253

Query: 256 LLFWSMKPDASLDPTSLHGACPVIKGNKWSCTKWMHVNKYS 292
           LLFWSMKPDASLDP+SLHG CPVIKGNKWS TKW HV++Y+
Sbjct: 254 LLFWSMKPDASLDPSSLHGGCPVIKGNKWSSTKWFHVHEYN 290

[Close]
BLAST of MS005262 vs. ExPASy Swiss-Prot
Match: Q24JN5 (Prolyl 4-hydroxylase 5 OS=Arabidopsis thaliana OX=3702 GN=P4H5 PE=2 SV=1)

HSP 1 Score: 359.4 bits (921), Expect = 3.9e-98
Identity = 174/274 (63.50%), Postives = 219/274 (79.93%), Query Frame = 0 

Query: 19  STLELSKIIMALVLALGFSMLLALRFFSPPESS-DPNLPDRLASVRRKAVESEGLGK-RG 78
           ST   + +I+ LV+ L   +LL L   S P ++ + +  + L ++ RK+  S G  +  G
Sbjct: 20  STQAFTVLILLLVVIL---ILLGLGILSLPNANRNSSKTNDLTNIVRKSETSSGDEEGNG 79

Query: 79  EQWVEVISWEPRAFIYHNFLSKEECLYLISLAKPRMVKSTVIDSETGKSMDSRVRTSSGM 138
           E+WVEVISWEPRA +YHNFL+ EEC +LISLAKP MVKSTV+D +TG S DSRVRTSSG 
Sbjct: 80  ERWVEVISWEPRAVVYHNFLTNEECEHLISLAKPSMVKSTVVDEKTGGSKDSRVRTSSGT 139

Query: 139 FLSRGQDRIIRNIEKRIADFTFIPIAEHGEGLQILHYEVGQKYDAHHDYFVDEYNIKKGS 198
           FL RG D ++  IEKRI+DFTFIP+ E+GEGLQ+LHY+VGQKY+ H+DYF+DE+N K G 
Sbjct: 140 FLRRGHDEVVEVIEKRISDFTFIPV-ENGEGLQVLHYQVGQKYEPHYDYFLDEFNTKNGG 199

Query: 199 QRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKMGDALLFWSM 258
           QR+AT+LMYLSDV++GGETVFPAA+GN S+VPWWNELS+CGK GLSV PK  DALLFW+M
Sbjct: 200 QRIATVLMYLSDVDDGGETVFPAARGNISAVPWWNELSKCGKEGLSVLPKKRDALLFWNM 259

Query: 259 KPDASLDPTSLHGACPVIKGNKWSCTKWMHVNKY 291
           +PDASLDP+SLHG CPV+KGNKWS TKW HV+++
Sbjct: 260 RPDASLDPSSLHGGCPVVKGNKWSSTKWFHVHEF 289

[Close]
BLAST of MS005262 vs. ExPASy Swiss-Prot
Match: Q8L970 (Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 2.4e-63
Identity = 113/211 (53.55%), Postives = 158/211 (74.88%), Query Frame = 0 

Query: 80  VEVISWEPRAFIYHNFLSKEECLYLISLAKPRMVKSTVIDSETGKSMDSRVRTSSGMFLS 139
           V  +SW PR F+Y  FLS EEC + I LAK ++ KS V D+++G+S++S VRTSSGMFLS
Sbjct: 56  VTQLSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEVRTSSGMFLS 115

Query: 140 RGQDRIIRNIEKRIADFTFIPIAEHGEGLQILHYEVGQKYDAHHDYFVDEYNIKKGSQRM 199
           + QD I+ N+E ++A +TF+P  E+GE +QILHYE GQKY+ H DYF D+ N++ G  R+
Sbjct: 116 KRQDDIVSNVEAKLAAWTFLP-EENGESMQILHYENGQKYEPHFDYFHDQANLELGGHRI 175

Query: 200 ATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKMGDALLFWSMKPD 259
           AT+LMYLS+VE+GGETVFP  KG  + +   +  +EC K G +VKP+ GDALLF+++ P+
Sbjct: 176 ATVLMYLSNVEKGGETVFPMWKGKATQLK-DDSWTECAKQGYAVKPRKGDALLFFNLHPN 235

Query: 260 ASLDPTSLHGACPVIKGNKWSCTKWMHVNKY 291
           A+ D  SLHG+CPV++G KWS T+W+HV  +
Sbjct: 236 ATTDSNSLHGSCPVVEGEKWSATRWIHVKSF 264

[Close]
BLAST of MS005262 vs. ExPASy TrEMBL
Match: A0A6J1CNS9 (probable prolyl 4-hydroxylase 3 OS=Momordica charantia OX=3673 GN=LOC111013165 PE=4 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 4.5e-166
Identity = 290/291 (99.66%), Postives = 290/291 (99.66%), Query Frame = 0 

Query: 1   MAVSKGKYGKINFHGRKWSTLELSKIIMALVLALGFSMLLALRFFSPPESSDPNLPDRLA 60
           MAVSKGKYGKINFHGRKWSTLELSKIIMALVLALGFSMLLALRFFSPPESSDPNLPDRLA
Sbjct: 1   MAVSKGKYGKINFHGRKWSTLELSKIIMALVLALGFSMLLALRFFSPPESSDPNLPDRLA 60

Query: 61  SVRRKAVESEGLGKRGEQWVEVISWEPRAFIYHNFLSKEECLYLISLAKPRMVKSTVIDS 120
           SVRRKAVESEGLGKRGEQWVEVISWEPRAFIYHNFLSKEECLYLISLAKPRMVKSTVIDS
Sbjct: 61  SVRRKAVESEGLGKRGEQWVEVISWEPRAFIYHNFLSKEECLYLISLAKPRMVKSTVIDS 120

Query: 121 ETGKSMDSRVRTSSGMFLSRGQDRIIRNIEKRIADFTFIPIAEHGEGLQILHYEVGQKYD 180
           ETGKSMDSRVRTSSGMFLSRGQDRIIRNIEKRIADFTFIPI EHGEGLQILHYEVGQKYD
Sbjct: 121 ETGKSMDSRVRTSSGMFLSRGQDRIIRNIEKRIADFTFIPI-EHGEGLQILHYEVGQKYD 180

Query: 181 AHHDYFVDEYNIKKGSQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGG 240
           AHHDYFVDEYNIKKGSQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGG
Sbjct: 181 AHHDYFVDEYNIKKGSQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGG 240

Query: 241 LSVKPKMGDALLFWSMKPDASLDPTSLHGACPVIKGNKWSCTKWMHVNKYS 292
           LSVKPKMGDALLFWSMKPDASLDPTSLHGACPVIKGNKWSCTKWMHVNKYS
Sbjct: 241 LSVKPKMGDALLFWSMKPDASLDPTSLHGACPVIKGNKWSCTKWMHVNKYS 290

[Close]
BLAST of MS005262 vs. ExPASy TrEMBL
Match: A0A1S3C2P6 (probable prolyl 4-hydroxylase 3 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103496331 PE=4 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 1.6e-142
Identity = 251/290 (86.55%), Postives = 268/290 (92.41%), Query Frame = 0 

Query: 1   MAVSKGKYGKINFHGRKWSTLELSKIIMALVLALGFSMLLALRFFSPPESSDPNLPDRLA 60
           MAVSKGKY  I   G+KWST +LSK+IMALVLALGF ML+ALRFFSPPE+S   LP    
Sbjct: 1   MAVSKGKY--IKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRLP---- 60

Query: 61  SVRRKAVESEGLGKRGEQWVEVISWEPRAFIYHNFLSKEECLYLISLAKPRMVKSTVIDS 120
           SVRR A +S+GLGKRG+QWVE ISWEPRAF+YHNFLSKEECLYLISLAKP M KSTV+DS
Sbjct: 61  SVRRTAFQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDS 120

Query: 121 ETGKSMDSRVRTSSGMFLSRGQDRIIRNIEKRIADFTFIPIAEHGEGLQILHYEVGQKYD 180
           +TGKS+DSRVRTSSGMFL+RGQD+II NIEKRIADFTFIPI EHGEGLQILHYEVGQKYD
Sbjct: 121 KTGKSVDSRVRTSSGMFLNRGQDKIISNIEKRIADFTFIPI-EHGEGLQILHYEVGQKYD 180

Query: 181 AHHDYFVDEYNIKKGSQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGG 240
           AH+DYFVDEYNIKKG QRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGG
Sbjct: 181 AHYDYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGG 240

Query: 241 LSVKPKMGDALLFWSMKPDASLDPTSLHGACPVIKGNKWSCTKWMHVNKY 291
           LSVKPKMGDALLFWSMKPDA+LDPTSLHGACPVI+GNKWSCTKWMHVNKY
Sbjct: 241 LSVKPKMGDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVNKY 283

[Close]
BLAST of MS005262 vs. ExPASy TrEMBL
Match: A0A6J1FBR3 (probable prolyl 4-hydroxylase 3 OS=Cucurbita moschata OX=3662 GN=LOC111444174 PE=4 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 7.5e-137
Identity = 237/290 (81.72%), Postives = 263/290 (90.69%), Query Frame = 0 

Query: 1   MAVSKGKYGKINFHGRKWSTLELSKIIMALVLALGFSMLLALRFFSPPESSDPNLPDRLA 60
           MAVSKGKY  + F  RKWST +LSKIIMA +LALG SML+A RFFSPPESS  NL  R+A
Sbjct: 1   MAVSKGKY--VKFQARKWSTFKLSKIIMAFLLALGVSMLIAFRFFSPPESSHSNLLHRVA 60

Query: 61  SVRRKAVESEGLGKRGEQWVEVISWEPRAFIYHNFLSKEECLYLISLAKPRMVKSTVIDS 120
           SV+ +AV S+GLGKRG+QWVE ISWEPRAF+YHNFLSKEECLYLISLAKP M KSTV+D+
Sbjct: 61  SVQHRAVHSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMAKSTVVDN 120

Query: 121 ETGKSMDSRVRTSSGMFLSRGQDRIIRNIEKRIADFTFIPIAEHGEGLQILHYEVGQKYD 180
           +TGKS+DSRVRTSSGMFL RGQ++I+ NIEKRIADFTFIP+ EHGE LQILHYEVGQKYD
Sbjct: 121 KTGKSIDSRVRTSSGMFLKRGQNKIVSNIEKRIADFTFIPV-EHGEALQILHYEVGQKYD 180

Query: 181 AHHDYFVDEYNIKKGSQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGG 240
           AHHDYF DE+NIK+G QRMATLLMYLSDVEEGGETVFPAA+GNFSS+P WNELSECGKGG
Sbjct: 181 AHHDYFADEFNIKQGGQRMATLLMYLSDVEEGGETVFPAAEGNFSSLPGWNELSECGKGG 240

Query: 241 LSVKPKMGDALLFWSMKPDASLDPTSLHGACPVIKGNKWSCTKWMHVNKY 291
           LSVKPKMGDALLFWSMKPD ++DPTSLHGACPVI+GNKWSCTKWM VN+Y
Sbjct: 241 LSVKPKMGDALLFWSMKPDNTVDPTSLHGACPVIRGNKWSCTKWMRVNEY 287

[Close]
BLAST of MS005262 vs. ExPASy TrEMBL
Match: A0A6J1HLH2 (probable prolyl 4-hydroxylase 3 OS=Cucurbita maxima OX=3661 GN=LOC111465626 PE=4 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 9.2e-135
Identity = 235/290 (81.03%), Postives = 260/290 (89.66%), Query Frame = 0 

Query: 1   MAVSKGKYGKINFHGRKWSTLELSKIIMALVLALGFSMLLALRFFSPPESSDPNLPDRLA 60
           MAVSKGKY K  F  RKWST +LSKIIM  +LALG SM +A RFFSPPESS   L  RLA
Sbjct: 1   MAVSKGKYMK--FQARKWSTFKLSKIIMVFLLALGVSMFIAFRFFSPPESSHSELLHRLA 60

Query: 61  SVRRKAVESEGLGKRGEQWVEVISWEPRAFIYHNFLSKEECLYLISLAKPRMVKSTVIDS 120
           SV+ +AV S+GLGKRG+QWVE ISWEPRAF+YHNFLSKEECLYLISLAKP M KSTV+D+
Sbjct: 61  SVQHRAVHSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPYMAKSTVVDN 120

Query: 121 ETGKSMDSRVRTSSGMFLSRGQDRIIRNIEKRIADFTFIPIAEHGEGLQILHYEVGQKYD 180
           +TGKS+DSRVRTSSGMFL RGQ++I+ NIEKRIADFTFIP+ EHGE LQILHYEVGQKYD
Sbjct: 121 KTGKSIDSRVRTSSGMFLRRGQNKIVSNIEKRIADFTFIPV-EHGEALQILHYEVGQKYD 180

Query: 181 AHHDYFVDEYNIKKGSQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGG 240
           AHHDYF DE+NIK+G QRMATLLMYLSDVEEGGETVFPAA+GNFSS+P WNELSECGKGG
Sbjct: 181 AHHDYFADEFNIKQGGQRMATLLMYLSDVEEGGETVFPAAEGNFSSLPGWNELSECGKGG 240

Query: 241 LSVKPKMGDALLFWSMKPDASLDPTSLHGACPVIKGNKWSCTKWMHVNKY 291
           LSVKPKMGDALLFWSM+PD ++DPTSLHGACPVI+GNKWSCTKWM VN+Y
Sbjct: 241 LSVKPKMGDALLFWSMRPDNTVDPTSLHGACPVIRGNKWSCTKWMRVNEY 287

[Close]
BLAST of MS005262 vs. ExPASy TrEMBL
Match: A0A1S4DZG7 (probable prolyl 4-hydroxylase 3 OS=Cucumis melo OX=3656 GN=LOC103496316 PE=4 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 5.0e-133
Identity = 237/290 (81.72%), Postives = 257/290 (88.62%), Query Frame = 0 

Query: 1   MAVSKGKYGKINFHGRKWSTLELSKIIMALVLALGFSMLLALRFFSPPESSDPNLPDRLA 60
           MAVS GKY  I   G+KWST +LSK+IMALVLALGF ML AL FFSPPE+S      RL+
Sbjct: 1   MAVSIGKY--IKLQGKKWSTFQLSKMIMALVLALGFFMLSALWFFSPPETSH----HRLS 60

Query: 61  SVRRKAVESEGLGKRGEQWVEVISWEPRAFIYHNFLSKEECLYLISLAKPRMVKSTVIDS 120
           SVR  A  S+GLGKRG+QWVE ISWEPRAF+YHNFLSKEECLYLISLAKP M KSTV+DS
Sbjct: 61  SVRHTAFLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDS 120

Query: 121 ETGKSMDSRVRTSSGMFLSRGQDRIIRNIEKRIADFTFIPIAEHGEGLQILHYEVGQKYD 180
           ETGKS+DS VRTSSGMFL+RGQD+II NIEKRIADFTFIPI EHGE +QILHY VGQKYD
Sbjct: 121 ETGKSVDSSVRTSSGMFLNRGQDKIISNIEKRIADFTFIPI-EHGEDIQILHYAVGQKYD 180

Query: 181 AHHDYFVDEYNIKKGSQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGG 240
           AH+D+FVDEYN+K   QRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGK G
Sbjct: 181 AHYDFFVDEYNLKSVGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKSG 240

Query: 241 LSVKPKMGDALLFWSMKPDASLDPTSLHGACPVIKGNKWSCTKWMHVNKY 291
           LS+KPKMGDALLFWSMKPD +LDPTSLHGACPVI+GNKWSCTKW+HVNKY
Sbjct: 241 LSIKPKMGDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNKY 283

[Close]
BLAST of MS005262 vs. TAIR 10
Match: AT1G20270.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 425.2 bits (1092), Expect = 4.1e-119
Identity = 207/279 (74.19%), Postives = 234/279 (83.87%), Query Frame = 0 

Query: 13  FHGRKWSTLELSKIIMALVLALGFSMLLALRFFSPPESSDPNLPDRLASVRRKAVE-SEG 72
           F  RKWSTL L  + M  +L +   MLLA   FS P ++D + P  L+  RR A E SEG
Sbjct: 9   FQARKWSTLML-VLFMLFMLTIVLLMLLAFGVFSLPINNDESSPIDLSYFRRAATERSEG 68

Query: 73  LGKRGEQWVEVISWEPRAFIYHNFLSKEECLYLISLAKPRMVKSTVIDSETGKSMDSRVR 132
           LGKRG+QW EV+SWEPRAF+YHNFLSKEEC YLISLAKP MVKSTV+DSETGKS DSRVR
Sbjct: 69  LGKRGDQWTEVLSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSETGKSKDSRVR 128

Query: 133 TSSGMFLSRGQDRIIRNIEKRIADFTFIPIAEHGEGLQILHYEVGQKYDAHHDYFVDEYN 192
           TSSG FL RG+D+II+ IEKRIAD+TFIP A+HGEGLQ+LHYE GQKY+ H+DYFVDE+N
Sbjct: 129 TSSGTFLRRGRDKIIKTIEKRIADYTFIP-ADHGEGLQVLHYEAGQKYEPHYDYFVDEFN 188

Query: 193 IKKGSQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKMGDAL 252
            K G QRMAT+LMYLSDVEEGGETVFPAA  NFSSVPW+NELSECGK GLSVKP+MGDAL
Sbjct: 189 TKNGGQRMATMLMYLSDVEEGGETVFPAANMNFSSVPWYNELSECGKKGLSVKPRMGDAL 248

Query: 253 LFWSMKPDASLDPTSLHGACPVIKGNKWSCTKWMHVNKY 291
           LFWSM+PDA+LDPTSLHG CPVI+GNKWS TKWMHV +Y
Sbjct: 249 LFWSMRPDATLDPTSLHGGCPVIRGNKWSSTKWMHVGEY 285

[Close]
BLAST of MS005262 vs. TAIR 10
Match: AT5G66060.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 382.5 bits (981), Expect = 3.0e-106
Identity = 184/274 (67.15%), Postives = 223/274 (81.39%), Query Frame = 0 

Query: 19  STLELSKIIMALVLALGFSMLLALRFFS-PPESSDPNLPDRLASVRRKAVESEGL-GKRG 78
           STL  + +IM+  + L   +LLA    S P  ++  +  + L S+ RK ++  G    + 
Sbjct: 18  STLVFAVLIMSTFVIL---ILLAFGILSVPSNNAGSSKANDLTSIVRKTLQRSGEDDSKN 77

Query: 79  EQWVEVISWEPRAFIYHNFLSKEECLYLISLAKPRMVKSTVIDSETGKSMDSRVRTSSGM 138
           E+WVE+ISWEPRA +YHNFL+KEEC YLI LAKP M KSTV+D +TGKS DSRVRTSSG 
Sbjct: 78  ERWVEIISWEPRASVYHNFLTKEECKYLIELAKPHMEKSTVVDEKTGKSTDSRVRTSSGT 137

Query: 139 FLSRGQDRIIRNIEKRIADFTFIPIAEHGEGLQILHYEVGQKYDAHHDYFVDEYNIKKGS 198
           FL+RG+D+ IR IEKRI+DFTFIP+ EHGEGLQ+LHYE+GQKY+ H+DYF+DEYN + G 
Sbjct: 138 FLARGRDKTIREIEKRISDFTFIPV-EHGEGLQVLHYEIGQKYEPHYDYFMDEYNTRNGG 197

Query: 199 QRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKMGDALLFWSM 258
           QR+AT+LMYLSDVEEGGETVFPAAKGN+S+VPWWNELSECGKGGLSVKPKMGDALLFWSM
Sbjct: 198 QRIATVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGGLSVKPKMGDALLFWSM 257

Query: 259 KPDASLDPTSLHGACPVIKGNKWSCTKWMHVNKY 291
            PDA+LDP+SLHG C VIKGNKWS TKW+ V++Y
Sbjct: 258 TPDATLDPSSLHGGCAVIKGNKWSSTKWLRVHEY 287

[Close]
BLAST of MS005262 vs. TAIR 10
Match: AT4G35810.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 367.9 bits (943), Expect = 7.8e-102
Identity = 176/281 (62.63%), Postives = 220/281 (78.29%), Query Frame = 0 

Query: 16  RKWSTLELSKIIMALVLALGFSMLLALRFFS-PPESSDPNLPDRLASVRRKAVESEGLGK 75
           + +ST   + +++ L + L   +L+ L  FS P  +   ++P  L ++ +   E E  G 
Sbjct: 14  KSFSTQTFTVVVLVLFVIL---ILVGLGIFSLPSTNKTSSMPMDLTTIVQTIQERESFGD 73

Query: 76  ----RGEQWVEVISWEPRAFIYHNFLSKEECLYLISLAKPRMVKSTVIDSETGKSMDSRV 135
                G++W+EVISWEPRAF+YHNFL+ EEC +LISLAKP M+KS V+D +TGKS+DSRV
Sbjct: 74  EEDGNGDRWLEVISWEPRAFVYHNFLTNEECEHLISLAKPSMMKSKVVDVKTGKSIDSRV 133

Query: 136 RTSSGMFLSRGQDRIIRNIEKRIADFTFIPIAEHGEGLQILHYEVGQKYDAHHDYFVDEY 195
           RTSSG FL+RG D I+  IE RI+DFTFIP  E+GEGLQ+LHYEVGQ+Y+ HHDYF DE+
Sbjct: 134 RTSSGTFLNRGHDEIVEEIENRISDFTFIP-PENGEGLQVLHYEVGQRYEPHHDYFFDEF 193

Query: 196 NIKKGSQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKMGDA 255
           N++KG QR+AT+LMYLSDV+EGGETVFPAAKGN S VPWW+ELS+CGK GLSV PK  DA
Sbjct: 194 NVRKGGQRIATVLMYLSDVDEGGETVFPAAKGNVSDVPWWDELSQCGKEGLSVLPKKRDA 253

Query: 256 LLFWSMKPDASLDPTSLHGACPVIKGNKWSCTKWMHVNKYS 292
           LLFWSMKPDASLDP+SLHG CPVIKGNKWS TKW HV++Y+
Sbjct: 254 LLFWSMKPDASLDPSSLHGGCPVIKGNKWSSTKWFHVHEYN 290

[Close]
BLAST of MS005262 vs. TAIR 10
Match: AT2G17720.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 359.4 bits (921), Expect = 2.8e-99
Identity = 174/274 (63.50%), Postives = 219/274 (79.93%), Query Frame = 0 

Query: 19  STLELSKIIMALVLALGFSMLLALRFFSPPESS-DPNLPDRLASVRRKAVESEGLGK-RG 78
           ST   + +I+ LV+ L   +LL L   S P ++ + +  + L ++ RK+  S G  +  G
Sbjct: 20  STQAFTVLILLLVVIL---ILLGLGILSLPNANRNSSKTNDLTNIVRKSETSSGDEEGNG 79

Query: 79  EQWVEVISWEPRAFIYHNFLSKEECLYLISLAKPRMVKSTVIDSETGKSMDSRVRTSSGM 138
           E+WVEVISWEPRA +YHNFL+ EEC +LISLAKP MVKSTV+D +TG S DSRVRTSSG 
Sbjct: 80  ERWVEVISWEPRAVVYHNFLTNEECEHLISLAKPSMVKSTVVDEKTGGSKDSRVRTSSGT 139

Query: 139 FLSRGQDRIIRNIEKRIADFTFIPIAEHGEGLQILHYEVGQKYDAHHDYFVDEYNIKKGS 198
           FL RG D ++  IEKRI+DFTFIP+ E+GEGLQ+LHY+VGQKY+ H+DYF+DE+N K G 
Sbjct: 140 FLRRGHDEVVEVIEKRISDFTFIPV-ENGEGLQVLHYQVGQKYEPHYDYFLDEFNTKNGG 199

Query: 199 QRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKMGDALLFWSM 258
           QR+AT+LMYLSDV++GGETVFPAA+GN S+VPWWNELS+CGK GLSV PK  DALLFW+M
Sbjct: 200 QRIATVLMYLSDVDDGGETVFPAARGNISAVPWWNELSKCGKEGLSVLPKKRDALLFWNM 259

Query: 259 KPDASLDPTSLHGACPVIKGNKWSCTKWMHVNKY 291
           +PDASLDP+SLHG CPV+KGNKWS TKW HV+++
Sbjct: 260 RPDASLDPSSLHGGCPVVKGNKWSSTKWFHVHEF 289

[Close]
BLAST of MS005262 vs. TAIR 10
Match: AT5G66060.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 284.6 bits (727), Expect = 8.7e-77
Identity = 143/224 (63.84%), Postives = 176/224 (78.57%), Query Frame = 0 

Query: 19  STLELSKIIMALVLALGFSMLLALRFFS-PPESSDPNLPDRLASVRRKAVESEGL-GKRG 78
           STL  + +IM+  + L   +LLA    S P  ++  +  + L S+ RK ++  G    + 
Sbjct: 18  STLVFAVLIMSTFVIL---ILLAFGILSVPSNNAGSSKANDLTSIVRKTLQRSGEDDSKN 77

Query: 79  EQWVEVISWEPRAFIYHNFLSKEECLYLISLAKPRMVKSTVIDSETGKSMDSRVRTSSGM 138
           E+WVE+ISWEPRA +YHNFL  EEC YLI LAKP M KSTV+D +TGKS DSRVRTSSG 
Sbjct: 78  ERWVEIISWEPRASVYHNFL--EECKYLIELAKPHMEKSTVVDEKTGKSTDSRVRTSSGT 137

Query: 139 FLSRGQDRIIRNIEKRIADFTFIPIAEHGEGLQILHYEVGQKYDAHHDYFVDEYNIKKGS 198
           FL+RG+D+ IR IEKRI+DFTFIP+ EHGEGLQ+LHYE+GQKY+ H+DYF+DEYN + G 
Sbjct: 138 FLARGRDKTIREIEKRISDFTFIPV-EHGEGLQVLHYEIGQKYEPHYDYFMDEYNTRNGG 197

Query: 199 QRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGG 241
           QR+AT+LMYLSDVEEGGETVFPAAKGN+S+VPWWNELSECGKGG
Sbjct: 198 QRIATVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGG 235

The following BLAST results are available for this feature:
BLAST of MS005262 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022143254.19.3e-16699.66probable prolyl 4-hydroxylase 3 [Momordica charantia][more]
XP_038889689.13.7e-14688.28probable prolyl 4-hydroxylase 3 [Benincasa hispida][more]
XP_008456388.13.2e-14286.55PREDICTED: probable prolyl 4-hydroxylase 3 isoform X2 [Cucumis melo][more]
XP_011648735.21.8e-14085.52probable prolyl 4-hydroxylase 3 [Cucumis sativus] >KAE8651549.1 hypothetical pro... [more]
XP_022937931.11.6e-13681.72probable prolyl 4-hydroxylase 3 [Cucurbita moschata][more]
BLAST of MS005262 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9LN205.8e-11874.19Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=... [more]
F4JZ244.3e-10567.15Probable prolyl 4-hydroxylase 10 OS=Arabidopsis thaliana OX=3702 GN=P4H10 PE=3 S... [more]
F4JNU81.1e-10062.63Probable prolyl 4-hydroxylase 8 OS=Arabidopsis thaliana OX=3702 GN=P4H8 PE=3 SV=... [more]
Q24JN53.9e-9863.50Prolyl 4-hydroxylase 5 OS=Arabidopsis thaliana OX=3702 GN=P4H5 PE=2 SV=1[more]
Q8L9702.4e-6353.55Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=... [more]
BLAST of MS005262 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1CNS94.5e-16699.66probable prolyl 4-hydroxylase 3 OS=Momordica charantia OX=3673 GN=LOC111013165 P... [more]
A0A1S3C2P61.6e-14286.55probable prolyl 4-hydroxylase 3 isoform X2 OS=Cucumis melo OX=3656 GN=LOC1034963... [more]
A0A6J1FBR37.5e-13781.72probable prolyl 4-hydroxylase 3 OS=Cucurbita moschata OX=3662 GN=LOC111444174 PE... [more]
A0A6J1HLH29.2e-13581.03probable prolyl 4-hydroxylase 3 OS=Cucurbita maxima OX=3661 GN=LOC111465626 PE=4... [more]
A0A1S4DZG75.0e-13381.72probable prolyl 4-hydroxylase 3 OS=Cucumis melo OX=3656 GN=LOC103496316 PE=4 SV=... [more]
BLAST of MS005262 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G20270.14.1e-11974.192-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G66060.13.0e-10667.152-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G35810.17.8e-10262.632-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT2G17720.12.8e-9963.502-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G66060.28.7e-7763.842-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006620Prolyl 4-hydroxylase, alpha subunitSMARTSM00702p4hccoord: 87..286
e-value: 1.3E-59
score: 214.1
NoneNo IPR availableGENE3D2.60.120.620q2cbj1_9rhob like domaincoord: 79..287
e-value: 3.1E-77
score: 261.0
NoneNo IPR availablePANTHERPTHR10869:SF169PROLYL 4-HYDROXYLASE 3-RELATEDcoord: 5..290
IPR044862Prolyl 4-hydroxylase alpha subunit, Fe(2+) 2OG dioxygenase domainPFAMPF136402OG-FeII_Oxy_3coord: 168..286
e-value: 1.4E-20
score: 74.0
IPR045054Prolyl 4-hydroxylasePANTHERPTHR10869PROLYL 4-HYDROXYLASE ALPHA SUBUNITcoord: 5..290
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 164..287
score: 12.2256

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS005262.1MS005262.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005506 iron ion binding
molecular_function GO:0031418 L-ascorbic acid binding
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen