MS004716 (gene) Bitter gourd (TR) v1

BLAST of MS004716 vs. NCBI nr
Match: XP_022150129.1 (uncharacterized protein LOC111018383 [Momordica charantia])

HSP 1 Score: 699.9 bits (1805), Expect = 1.2e-197
Identity = 362/363 (99.72%), Postives = 362/363 (99.72%), Query Frame = 0

Query: 1   MAASARAFFLSRVTDFSIKPRLPPQPPPPPSLPSFSSPHLGVLRRRFTSSSGATTVSCLV 60
           MAASARAFFLSRVTDFSIKPRLPPQPPPPPSLPSFSSPHLGVLRRRFTSSSGATTVSCLV
Sbjct: 1   MAASARAFFLSRVTDFSIKPRLPPQPPPPPSLPSFSSPHLGVLRRRFTSSSGATTVSCLV 60

Query: 61  SGVDGGGVSDDFVSTRKLKFDRGFSVIANMLKRIEPLDTSDISKGVSDAAKDSMKQTISS 120
           SGVDGGGVSDDFVSTRKLKFDRGFSVIANMLKRIEPLDTSDIS GVSDAAKDSMKQTISS
Sbjct: 61  SGVDGGGVSDDFVSTRKLKFDRGFSVIANMLKRIEPLDTSDISNGVSDAAKDSMKQTISS 120

Query: 121 MLGLLPSDQFAVTVRVSKNPLHNILSSSIITGYTLWNAEYRLSLMRNFDISPDNLTGLNR 180
           MLGLLPSDQFAVTVRVSKNPLHNILSSSIITGYTLWNAEYRLSLMRNFDISPDNLTGLNR
Sbjct: 121 MLGLLPSDQFAVTVRVSKNPLHNILSSSIITGYTLWNAEYRLSLMRNFDISPDNLTGLNR 180

Query: 181 SKPMEVSDVEETLVGVDSDVEDLDRTRPRLLTDLPPEALKYIQQLQSELSNLKDELNTRK 240
           SKPMEVSDVEETLVGVDSDVEDLDRTRPRLLTDLPPEALKYIQQLQSELSNLKDELNTRK
Sbjct: 181 SKPMEVSDVEETLVGVDSDVEDLDRTRPRLLTDLPPEALKYIQQLQSELSNLKDELNTRK 240

Query: 241 QENMQMEYGRGNRNNLLEYLRSLDSNMVTELCKPSTLEVEEIIHELVGNILQRFFKDDAS 300
           QENMQMEYGRGNRNNLLEYLRSLDSNMVTELCKPSTLEVEEIIHELVGNILQRFFKDDAS
Sbjct: 241 QENMQMEYGRGNRNNLLEYLRSLDSNMVTELCKPSTLEVEEIIHELVGNILQRFFKDDAS 300

Query: 301 STFVEDSSVADLGKLANAGDEFYDTVGTSRDYLAKLLFWCMLLGHHLRSLENRLQLSCVV 360
           STFVEDSSVADLGKLANAGDEFYDTVGTSRDYLAKLLFWCMLLGHHLRSLENRLQLSCVV
Sbjct: 301 STFVEDSSVADLGKLANAGDEFYDTVGTSRDYLAKLLFWCMLLGHHLRSLENRLQLSCVV 360

Query: 361 GLL 364
           GLL
Sbjct: 361 GLL 363

BLAST of MS004716 vs. NCBI nr
Match: XP_008443458.1 (PREDICTED: uncharacterized protein LOC103487044 [Cucumis melo] >KAA0053716.1 uncharacterized protein E6C27_scaffold135G00920 [Cucumis melo var. makuwa] >TYK17742.1 uncharacterized protein E5676_scaffold1804G00130 [Cucumis melo var. makuwa])

HSP 1 Score: 627.1 bits (1616), Expect = 9.5e-176
Identity = 327/364 (89.84%), Postives = 347/364 (95.33%), Query Frame = 0

Query: 1   MAASARAFFLSRVTDFSIKPRLPPQ-PPPPPSLPSFSSPHLGVLRRRFTSSSGATTVSCL 60
           MAASARAFFLSRVTDFSIKPRLPPQ PPPPP LPSFS  HL + RRRF S+SGATTVSCL
Sbjct: 1   MAASARAFFLSRVTDFSIKPRLPPQPPPPPPPLPSFSYSHLTLQRRRFPSTSGATTVSCL 60

Query: 61  VSGVDGGGVSDDFVSTRKLKFDRGFSVIANMLKRIEPLDTSDISKGVSDAAKDSMKQTIS 120
           VSGVDGGGVSDDFVSTRKLKFDRGFSVIANMLKRIEPL TSDISKGVSD AKDSMKQTIS
Sbjct: 61  VSGVDGGGVSDDFVSTRKLKFDRGFSVIANMLKRIEPLHTSDISKGVSDVAKDSMKQTIS 120

Query: 121 SMLGLLPSDQFAVTVRVSKNPLHNILSSSIITGYTLWNAEYRLSLMRNFDISPDNLTGLN 180
           SMLGLLPSDQF+VTVRVSK+PLHN+LSSSIITGYTLWNAEYRLSLMRNFDISPDNL GL+
Sbjct: 121 SMLGLLPSDQFSVTVRVSKSPLHNLLSSSIITGYTLWNAEYRLSLMRNFDISPDNLMGLD 180

Query: 181 RSKPMEVSDVEETLVGVDSDVEDLDRTRPRLLTDLPPEALKYIQQLQSELSNLKDELNTR 240
           RSKP+EVSD+EE LVGVDS++EDLD TRPRLL+DLPPEALKYI+QLQ+ELSNLKDELN +
Sbjct: 181 RSKPLEVSDIEENLVGVDSNMEDLD-TRPRLLSDLPPEALKYIEQLQTELSNLKDELNAQ 240

Query: 241 KQENMQMEYGRGNRNNLLEYLRSLDSNMVTELCKPSTLEVEEIIHELVGNILQRFFKDDA 300
           KQEN Q+E+GRGNRN+LLEYLRSLDSNMVTELCKPST EVEEIIHELVGNILQRFFKDDA
Sbjct: 241 KQENFQIEHGRGNRNDLLEYLRSLDSNMVTELCKPSTSEVEEIIHELVGNILQRFFKDDA 300

Query: 301 SSTFVEDSSVADLGKLANAGDEFYDTVGTSRDYLAKLLFWCMLLGHHLRSLENRLQLSCV 360
           SS+F+EDSSVADL KLA+AGDEF DTVGTSRDYLAKLLFWCMLLGHHLRSLENRLQLSCV
Sbjct: 301 SSSFIEDSSVADLEKLADAGDEFCDTVGTSRDYLAKLLFWCMLLGHHLRSLENRLQLSCV 360

Query: 361 VGLL 364
           VGLL
Sbjct: 361 VGLL 363

BLAST of MS004716 vs. NCBI nr
Match: XP_038905998.1 (uncharacterized protein LOC120091907 [Benincasa hispida])

HSP 1 Score: 626.7 bits (1615), Expect = 1.2e-175
Identity = 328/364 (90.11%), Postives = 344/364 (94.51%), Query Frame = 0

Query: 1   MAASARAFFLSRVTDFSIKPRLPPQ-PPPPPSLPSFSSPHLGVLRRRFTSSSGATTVSCL 60
           MA+SAR FFLSRVTDFSIKPRLPPQ PPPPP LPSFS  HL   RRRF S+SGATTVSCL
Sbjct: 1   MASSARTFFLSRVTDFSIKPRLPPQPPPPPPPLPSFSFSHLSFHRRRFPSTSGATTVSCL 60

Query: 61  VSGVDGGGVSDDFVSTRKLKFDRGFSVIANMLKRIEPLDTSDISKGVSDAAKDSMKQTIS 120
           +SGVDGGGVSDDFVSTRKLKFDRGFSVIANMLKRIEPLDTSDISKGVSD AKDSMKQTIS
Sbjct: 61  ISGVDGGGVSDDFVSTRKLKFDRGFSVIANMLKRIEPLDTSDISKGVSDVAKDSMKQTIS 120

Query: 121 SMLGLLPSDQFAVTVRVSKNPLHNILSSSIITGYTLWNAEYRLSLMRNFDISPDNLTGLN 180
           SMLGLLPSDQF+VTVRVSK+PLHN+LSSSIITGYTLWNAEYRLSLMRNFDISPD LTGL 
Sbjct: 121 SMLGLLPSDQFSVTVRVSKSPLHNLLSSSIITGYTLWNAEYRLSLMRNFDISPDKLTGLE 180

Query: 181 RSKPMEVSDVEETLVGVDSDVEDLDRTRPRLLTDLPPEALKYIQQLQSELSNLKDELNTR 240
           RSK +EVSD+EE  VGVDS++EDLD TRPRLLTDLPPEALKYIQQLQSELSNLKDELN R
Sbjct: 181 RSKSLEVSDIEEIRVGVDSNMEDLD-TRPRLLTDLPPEALKYIQQLQSELSNLKDELNAR 240

Query: 241 KQENMQMEYGRGNRNNLLEYLRSLDSNMVTELCKPSTLEVEEIIHELVGNILQRFFKDDA 300
           KQENMQ+E+GRGNRN+LLEYLRSLDSNMVTELCKPSTLEVEEIIHELVGNILQRFFKDDA
Sbjct: 241 KQENMQIEHGRGNRNDLLEYLRSLDSNMVTELCKPSTLEVEEIIHELVGNILQRFFKDDA 300

Query: 301 SSTFVEDSSVADLGKLANAGDEFYDTVGTSRDYLAKLLFWCMLLGHHLRSLENRLQLSCV 360
           SSTF+E SSVADL KLA+AG+EF DTVGTSRDYLAKLLFWCMLLGHHLRSLENRLQLSCV
Sbjct: 301 SSTFIEHSSVADLEKLADAGNEFCDTVGTSRDYLAKLLFWCMLLGHHLRSLENRLQLSCV 360

Query: 361 VGLL 364
           VGLL
Sbjct: 361 VGLL 363

BLAST of MS004716 vs. NCBI nr
Match: XP_011652254.1 (uncharacterized protein LOC101208572 [Cucumis sativus] >KGN59616.1 hypothetical protein Csa_001093 [Cucumis sativus])

HSP 1 Score: 616.7 bits (1589), Expect = 1.3e-172
Identity = 323/366 (88.25%), Postives = 346/366 (94.54%), Query Frame = 0

Query: 1   MAASARAFFLSRVTDFSIKPRLPPQ-PPPPPSLPSFSSPHLGVLRRRF--TSSSGATTVS 60
           MA SARAFFLSR+TDFSIKPRLPPQ PPPPP LPSFS  HL + RRRF   S+SGATTVS
Sbjct: 1   MAVSARAFFLSRLTDFSIKPRLPPQPPPPPPPLPSFSYSHLTLQRRRFPSASTSGATTVS 60

Query: 61  CLVSGVDGGGVSDDFVSTRKLKFDRGFSVIANMLKRIEPLDTSDISKGVSDAAKDSMKQT 120
           CLVSGVDGGGVSDDFVSTRKLKFDRGFSVIANMLKRIEPL TSDISKGVSD AKDSMKQT
Sbjct: 61  CLVSGVDGGGVSDDFVSTRKLKFDRGFSVIANMLKRIEPLHTSDISKGVSDVAKDSMKQT 120

Query: 121 ISSMLGLLPSDQFAVTVRVSKNPLHNILSSSIITGYTLWNAEYRLSLMRNFDISPDNLTG 180
           ISSMLGLLPSDQF+VTVRVSK+PLHN+LSSSIITGYTLWNAEYRLSLMRNFDISPDNLTG
Sbjct: 121 ISSMLGLLPSDQFSVTVRVSKSPLHNLLSSSIITGYTLWNAEYRLSLMRNFDISPDNLTG 180

Query: 181 LNRSKPMEVSDVEETLVGVDSDVEDLDRTRPRLLTDLPPEALKYIQQLQSELSNLKDELN 240
           L+RSKP+EVSD+EE  VGVDS++EDLD TRPRLL+DLPPEALKYIQQLQ+ELSNLKDELN
Sbjct: 181 LDRSKPLEVSDIEENRVGVDSNMEDLD-TRPRLLSDLPPEALKYIQQLQTELSNLKDELN 240

Query: 241 TRKQENMQMEYGRGNRNNLLEYLRSLDSNMVTELCKPSTLEVEEIIHELVGNILQRFFKD 300
            +KQEN+ +E+GRGNRN+LLEYLRSLDS+MVTELCKPST EVEEIIHELVGNILQRFFKD
Sbjct: 241 AQKQENIHIEHGRGNRNDLLEYLRSLDSDMVTELCKPSTSEVEEIIHELVGNILQRFFKD 300

Query: 301 DASSTFVEDSSVADLGKLANAGDEFYDTVGTSRDYLAKLLFWCMLLGHHLRSLENRLQLS 360
           DASS+F+EDSSVADL KLA+AGDEF DTVGTSRDYLAKLLFWCMLLGHH+RSLENRLQLS
Sbjct: 301 DASSSFIEDSSVADLEKLADAGDEFCDTVGTSRDYLAKLLFWCMLLGHHMRSLENRLQLS 360

Query: 361 CVVGLL 364
           CVVGLL
Sbjct: 361 CVVGLL 365

BLAST of MS004716 vs. NCBI nr
Match: XP_023531898.1 (uncharacterized protein LOC111794025 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 614.4 bits (1583), Expect = 6.4e-172
Identity = 324/364 (89.01%), Postives = 336/364 (92.31%), Query Frame = 0

Query: 1   MAASARAFFLSRVTDFSIKPRLPP-QPPPPPSLPSFSSPHLGVLRRRFTSSSGATTVSCL 60
           MAASAR  FLSRVTDFSIKPRL P  PPPPP LPSFS  HLGV RRRFT    A TVSCL
Sbjct: 1   MAASARFVFLSRVTDFSIKPRLSPLPPPPPPPLPSFSYSHLGVQRRRFT----AATVSCL 60

Query: 61  VSGVDGGGVSDDFVSTRKLKFDRGFSVIANMLKRIEPLDTSDISKGVSDAAKDSMKQTIS 120
           +SGVDGGGVSDDFVSTRKLKFDRGFSVIANMLKRIEPLDTSDISKGV+DAAKDSMKQTIS
Sbjct: 61  ISGVDGGGVSDDFVSTRKLKFDRGFSVIANMLKRIEPLDTSDISKGVTDAAKDSMKQTIS 120

Query: 121 SMLGLLPSDQFAVTVRVSKNPLHNILSSSIITGYTLWNAEYRLSLMRNFDISPDNLTGLN 180
           SM GLLPSDQFAVTVRV K+ LHN+LSSSIITGYTLWNAEYRLSLMRNFDISPD LTGL+
Sbjct: 121 SMFGLLPSDQFAVTVRVCKSSLHNLLSSSIITGYTLWNAEYRLSLMRNFDISPDRLTGLD 180

Query: 181 RSKPMEVSDVEETLVGVDSDVEDLDRTRPRLLTDLPPEALKYIQQLQSELSNLKDELNTR 240
           RSKP++VSD EETLVG+DSD+ED D TRPRLL DLPPEALKYIQQLQSELSNLKDELN R
Sbjct: 181 RSKPLDVSDDEETLVGIDSDMEDWDTTRPRLLADLPPEALKYIQQLQSELSNLKDELNAR 240

Query: 241 KQENMQMEYGRGNRNNLLEYLRSLDSNMVTELCKPSTLEVEEIIHELVGNILQRFFKDDA 300
           KQENMQME+GRGNRNNLLEYLRSLDSNMVTEL KPST EVEEIIHELVGNI+QRFFKDDA
Sbjct: 241 KQENMQMEHGRGNRNNLLEYLRSLDSNMVTELSKPSTSEVEEIIHELVGNIVQRFFKDDA 300

Query: 301 SSTFVEDSSVADLGKLANAGDEFYDTVGTSRDYLAKLLFWCMLLGHHLRSLENRLQLSCV 360
           SSTF EDSSVADL KLANAG+EFYDTVGTSRDYLAKLLFWCMLLGH LRSLENRLQLSCV
Sbjct: 301 SSTFFEDSSVADLEKLANAGNEFYDTVGTSRDYLAKLLFWCMLLGHRLRSLENRLQLSCV 360

Query: 361 VGLL 364
           VGLL
Sbjct: 361 VGLL 360

BLAST of MS004716 vs. ExPASy TrEMBL
Match: A0A6J1D928 (uncharacterized protein LOC111018383 OS=Momordica charantia OX=3673 GN=LOC111018383 PE=4 SV=1)

HSP 1 Score: 699.9 bits (1805), Expect = 5.6e-198
Identity = 362/363 (99.72%), Postives = 362/363 (99.72%), Query Frame = 0

Query: 1   MAASARAFFLSRVTDFSIKPRLPPQPPPPPSLPSFSSPHLGVLRRRFTSSSGATTVSCLV 60
           MAASARAFFLSRVTDFSIKPRLPPQPPPPPSLPSFSSPHLGVLRRRFTSSSGATTVSCLV
Sbjct: 1   MAASARAFFLSRVTDFSIKPRLPPQPPPPPSLPSFSSPHLGVLRRRFTSSSGATTVSCLV 60

Query: 61  SGVDGGGVSDDFVSTRKLKFDRGFSVIANMLKRIEPLDTSDISKGVSDAAKDSMKQTISS 120
           SGVDGGGVSDDFVSTRKLKFDRGFSVIANMLKRIEPLDTSDIS GVSDAAKDSMKQTISS
Sbjct: 61  SGVDGGGVSDDFVSTRKLKFDRGFSVIANMLKRIEPLDTSDISNGVSDAAKDSMKQTISS 120

Query: 121 MLGLLPSDQFAVTVRVSKNPLHNILSSSIITGYTLWNAEYRLSLMRNFDISPDNLTGLNR 180
           MLGLLPSDQFAVTVRVSKNPLHNILSSSIITGYTLWNAEYRLSLMRNFDISPDNLTGLNR
Sbjct: 121 MLGLLPSDQFAVTVRVSKNPLHNILSSSIITGYTLWNAEYRLSLMRNFDISPDNLTGLNR 180

Query: 181 SKPMEVSDVEETLVGVDSDVEDLDRTRPRLLTDLPPEALKYIQQLQSELSNLKDELNTRK 240
           SKPMEVSDVEETLVGVDSDVEDLDRTRPRLLTDLPPEALKYIQQLQSELSNLKDELNTRK
Sbjct: 181 SKPMEVSDVEETLVGVDSDVEDLDRTRPRLLTDLPPEALKYIQQLQSELSNLKDELNTRK 240

Query: 241 QENMQMEYGRGNRNNLLEYLRSLDSNMVTELCKPSTLEVEEIIHELVGNILQRFFKDDAS 300
           QENMQMEYGRGNRNNLLEYLRSLDSNMVTELCKPSTLEVEEIIHELVGNILQRFFKDDAS
Sbjct: 241 QENMQMEYGRGNRNNLLEYLRSLDSNMVTELCKPSTLEVEEIIHELVGNILQRFFKDDAS 300

Query: 301 STFVEDSSVADLGKLANAGDEFYDTVGTSRDYLAKLLFWCMLLGHHLRSLENRLQLSCVV 360
           STFVEDSSVADLGKLANAGDEFYDTVGTSRDYLAKLLFWCMLLGHHLRSLENRLQLSCVV
Sbjct: 301 STFVEDSSVADLGKLANAGDEFYDTVGTSRDYLAKLLFWCMLLGHHLRSLENRLQLSCVV 360

Query: 361 GLL 364
           GLL
Sbjct: 361 GLL 363

BLAST of MS004716 vs. ExPASy TrEMBL
Match: A0A5A7UHK4 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1804G00130 PE=4 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 4.6e-176
Identity = 327/364 (89.84%), Postives = 347/364 (95.33%), Query Frame = 0

Query: 1   MAASARAFFLSRVTDFSIKPRLPPQ-PPPPPSLPSFSSPHLGVLRRRFTSSSGATTVSCL 60
           MAASARAFFLSRVTDFSIKPRLPPQ PPPPP LPSFS  HL + RRRF S+SGATTVSCL
Sbjct: 1   MAASARAFFLSRVTDFSIKPRLPPQPPPPPPPLPSFSYSHLTLQRRRFPSTSGATTVSCL 60

Query: 61  VSGVDGGGVSDDFVSTRKLKFDRGFSVIANMLKRIEPLDTSDISKGVSDAAKDSMKQTIS 120
           VSGVDGGGVSDDFVSTRKLKFDRGFSVIANMLKRIEPL TSDISKGVSD AKDSMKQTIS
Sbjct: 61  VSGVDGGGVSDDFVSTRKLKFDRGFSVIANMLKRIEPLHTSDISKGVSDVAKDSMKQTIS 120

Query: 121 SMLGLLPSDQFAVTVRVSKNPLHNILSSSIITGYTLWNAEYRLSLMRNFDISPDNLTGLN 180
           SMLGLLPSDQF+VTVRVSK+PLHN+LSSSIITGYTLWNAEYRLSLMRNFDISPDNL GL+
Sbjct: 121 SMLGLLPSDQFSVTVRVSKSPLHNLLSSSIITGYTLWNAEYRLSLMRNFDISPDNLMGLD 180

Query: 181 RSKPMEVSDVEETLVGVDSDVEDLDRTRPRLLTDLPPEALKYIQQLQSELSNLKDELNTR 240
           RSKP+EVSD+EE LVGVDS++EDLD TRPRLL+DLPPEALKYI+QLQ+ELSNLKDELN +
Sbjct: 181 RSKPLEVSDIEENLVGVDSNMEDLD-TRPRLLSDLPPEALKYIEQLQTELSNLKDELNAQ 240

Query: 241 KQENMQMEYGRGNRNNLLEYLRSLDSNMVTELCKPSTLEVEEIIHELVGNILQRFFKDDA 300
           KQEN Q+E+GRGNRN+LLEYLRSLDSNMVTELCKPST EVEEIIHELVGNILQRFFKDDA
Sbjct: 241 KQENFQIEHGRGNRNDLLEYLRSLDSNMVTELCKPSTSEVEEIIHELVGNILQRFFKDDA 300

Query: 301 SSTFVEDSSVADLGKLANAGDEFYDTVGTSRDYLAKLLFWCMLLGHHLRSLENRLQLSCV 360
           SS+F+EDSSVADL KLA+AGDEF DTVGTSRDYLAKLLFWCMLLGHHLRSLENRLQLSCV
Sbjct: 301 SSSFIEDSSVADLEKLADAGDEFCDTVGTSRDYLAKLLFWCMLLGHHLRSLENRLQLSCV 360

Query: 361 VGLL 364
           VGLL
Sbjct: 361 VGLL 363

BLAST of MS004716 vs. ExPASy TrEMBL
Match: A0A1S3B853 (uncharacterized protein LOC103487044 OS=Cucumis melo OX=3656 GN=LOC103487044 PE=4 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 4.6e-176
Identity = 327/364 (89.84%), Postives = 347/364 (95.33%), Query Frame = 0

Query: 1   MAASARAFFLSRVTDFSIKPRLPPQ-PPPPPSLPSFSSPHLGVLRRRFTSSSGATTVSCL 60
           MAASARAFFLSRVTDFSIKPRLPPQ PPPPP LPSFS  HL + RRRF S+SGATTVSCL
Sbjct: 1   MAASARAFFLSRVTDFSIKPRLPPQPPPPPPPLPSFSYSHLTLQRRRFPSTSGATTVSCL 60

Query: 61  VSGVDGGGVSDDFVSTRKLKFDRGFSVIANMLKRIEPLDTSDISKGVSDAAKDSMKQTIS 120
           VSGVDGGGVSDDFVSTRKLKFDRGFSVIANMLKRIEPL TSDISKGVSD AKDSMKQTIS
Sbjct: 61  VSGVDGGGVSDDFVSTRKLKFDRGFSVIANMLKRIEPLHTSDISKGVSDVAKDSMKQTIS 120

Query: 121 SMLGLLPSDQFAVTVRVSKNPLHNILSSSIITGYTLWNAEYRLSLMRNFDISPDNLTGLN 180
           SMLGLLPSDQF+VTVRVSK+PLHN+LSSSIITGYTLWNAEYRLSLMRNFDISPDNL GL+
Sbjct: 121 SMLGLLPSDQFSVTVRVSKSPLHNLLSSSIITGYTLWNAEYRLSLMRNFDISPDNLMGLD 180

Query: 181 RSKPMEVSDVEETLVGVDSDVEDLDRTRPRLLTDLPPEALKYIQQLQSELSNLKDELNTR 240
           RSKP+EVSD+EE LVGVDS++EDLD TRPRLL+DLPPEALKYI+QLQ+ELSNLKDELN +
Sbjct: 181 RSKPLEVSDIEENLVGVDSNMEDLD-TRPRLLSDLPPEALKYIEQLQTELSNLKDELNAQ 240

Query: 241 KQENMQMEYGRGNRNNLLEYLRSLDSNMVTELCKPSTLEVEEIIHELVGNILQRFFKDDA 300
           KQEN Q+E+GRGNRN+LLEYLRSLDSNMVTELCKPST EVEEIIHELVGNILQRFFKDDA
Sbjct: 241 KQENFQIEHGRGNRNDLLEYLRSLDSNMVTELCKPSTSEVEEIIHELVGNILQRFFKDDA 300

Query: 301 SSTFVEDSSVADLGKLANAGDEFYDTVGTSRDYLAKLLFWCMLLGHHLRSLENRLQLSCV 360
           SS+F+EDSSVADL KLA+AGDEF DTVGTSRDYLAKLLFWCMLLGHHLRSLENRLQLSCV
Sbjct: 301 SSSFIEDSSVADLEKLADAGDEFCDTVGTSRDYLAKLLFWCMLLGHHLRSLENRLQLSCV 360

Query: 361 VGLL 364
           VGLL
Sbjct: 361 VGLL 363

BLAST of MS004716 vs. ExPASy TrEMBL
Match: A0A0A0LHU8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G829060 PE=4 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 6.2e-173
Identity = 323/366 (88.25%), Postives = 346/366 (94.54%), Query Frame = 0

Query: 1   MAASARAFFLSRVTDFSIKPRLPPQ-PPPPPSLPSFSSPHLGVLRRRF--TSSSGATTVS 60
           MA SARAFFLSR+TDFSIKPRLPPQ PPPPP LPSFS  HL + RRRF   S+SGATTVS
Sbjct: 1   MAVSARAFFLSRLTDFSIKPRLPPQPPPPPPPLPSFSYSHLTLQRRRFPSASTSGATTVS 60

Query: 61  CLVSGVDGGGVSDDFVSTRKLKFDRGFSVIANMLKRIEPLDTSDISKGVSDAAKDSMKQT 120
           CLVSGVDGGGVSDDFVSTRKLKFDRGFSVIANMLKRIEPL TSDISKGVSD AKDSMKQT
Sbjct: 61  CLVSGVDGGGVSDDFVSTRKLKFDRGFSVIANMLKRIEPLHTSDISKGVSDVAKDSMKQT 120

Query: 121 ISSMLGLLPSDQFAVTVRVSKNPLHNILSSSIITGYTLWNAEYRLSLMRNFDISPDNLTG 180
           ISSMLGLLPSDQF+VTVRVSK+PLHN+LSSSIITGYTLWNAEYRLSLMRNFDISPDNLTG
Sbjct: 121 ISSMLGLLPSDQFSVTVRVSKSPLHNLLSSSIITGYTLWNAEYRLSLMRNFDISPDNLTG 180

Query: 181 LNRSKPMEVSDVEETLVGVDSDVEDLDRTRPRLLTDLPPEALKYIQQLQSELSNLKDELN 240
           L+RSKP+EVSD+EE  VGVDS++EDLD TRPRLL+DLPPEALKYIQQLQ+ELSNLKDELN
Sbjct: 181 LDRSKPLEVSDIEENRVGVDSNMEDLD-TRPRLLSDLPPEALKYIQQLQTELSNLKDELN 240

Query: 241 TRKQENMQMEYGRGNRNNLLEYLRSLDSNMVTELCKPSTLEVEEIIHELVGNILQRFFKD 300
            +KQEN+ +E+GRGNRN+LLEYLRSLDS+MVTELCKPST EVEEIIHELVGNILQRFFKD
Sbjct: 241 AQKQENIHIEHGRGNRNDLLEYLRSLDSDMVTELCKPSTSEVEEIIHELVGNILQRFFKD 300

Query: 301 DASSTFVEDSSVADLGKLANAGDEFYDTVGTSRDYLAKLLFWCMLLGHHLRSLENRLQLS 360
           DASS+F+EDSSVADL KLA+AGDEF DTVGTSRDYLAKLLFWCMLLGHH+RSLENRLQLS
Sbjct: 301 DASSSFIEDSSVADLEKLADAGDEFCDTVGTSRDYLAKLLFWCMLLGHHMRSLENRLQLS 360

Query: 361 CVVGLL 364
           CVVGLL
Sbjct: 361 CVVGLL 365

BLAST of MS004716 vs. ExPASy TrEMBL
Match: A0A6J1J6Z6 (uncharacterized protein LOC111482318 OS=Cucurbita maxima OX=3661 GN=LOC111482318 PE=4 SV=1)

HSP 1 Score: 612.8 bits (1579), Expect = 9.0e-172
Identity = 322/363 (88.71%), Postives = 340/363 (93.66%), Query Frame = 0

Query: 1   MAASARAFFLSRVTDFSIKPRLPPQPPPPPSLPSFSSPHLGVLRRRFTSSSGATTVSCLV 60
           MAASARAFFLSR+TDFSIKPRLP  PPPPPSLPSFS  HLG+ RRRF  +SGATTVSCL+
Sbjct: 1   MAASARAFFLSRITDFSIKPRLP--PPPPPSLPSFSYSHLGLQRRRFPPTSGATTVSCLI 60

Query: 61  SGVDGGGVSDDFVSTRKLKFDRGFSVIANMLKRIEPLDTSDISKGVSDAAKDSMKQTISS 120
           SGVDGGGVSDDFVSTRKLKFDRGFSVIANMLKRIEPLDTS+IS+GVSDAAKDSMKQTISS
Sbjct: 61  SGVDGGGVSDDFVSTRKLKFDRGFSVIANMLKRIEPLDTSNISQGVSDAAKDSMKQTISS 120

Query: 121 MLGLLPSDQFAVTVRVSKNPLHNILSSSIITGYTLWNAEYRLSLMRNFDISPDNLTGLNR 180
           MLGLLPSDQFAVTVRVSK+PLHN+LSSSIITGYTLWNAEYRLSL RNFDISPDNLTG +R
Sbjct: 121 MLGLLPSDQFAVTVRVSKSPLHNLLSSSIITGYTLWNAEYRLSLTRNFDISPDNLTGFDR 180

Query: 181 SKPMEVSDVEETLVGVDSDVEDLDRTRPRLLTDLPPEALKYIQQLQSELSNLKDELNTRK 240
           SKP+EVSDV+E  VGVDS++EDLD TRPRLLTD  PEALKYIQQLQSELSNLKDELN +K
Sbjct: 181 SKPLEVSDVQEIRVGVDSNLEDLD-TRPRLLTDFSPEALKYIQQLQSELSNLKDELNAQK 240

Query: 241 QENMQMEYGRGNRNNLLEYLRSLDSNMVTELCKPSTLEVEEIIHELVGNILQRFFKDDAS 300
           Q NMQME+G+ NRN+LLEYLRSLDSNMVTELCKPST EVEEIIHELVGNILQRFFKDDAS
Sbjct: 241 QVNMQMEHGKENRNDLLEYLRSLDSNMVTELCKPSTSEVEEIIHELVGNILQRFFKDDAS 300

Query: 301 STFVEDSSVADLGKLANAGDEFYDTVGTSRDYLAKLLFWCMLLGHHLRSLENRLQLSCVV 360
           STF EDSSVADL KLA+ G EF DTVGTSRDYLAKLLFWCMLLGHHLRSLENRLQLSCVV
Sbjct: 301 STFNEDSSVADLEKLADVGSEFCDTVGTSRDYLAKLLFWCMLLGHHLRSLENRLQLSCVV 360

Query: 361 GLL 364
           GLL
Sbjct: 361 GLL 360

BLAST of MS004716 vs. TAIR 10
Match: AT5G14970.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G14910.1); Has 579 Blast hits to 397 proteins in 95 species: Archae - 0; Bacteria - 294; Metazoa - 0; Fungi - 0; Plants - 86; Viruses - 0; Other Eukaryotes - 199 (source: NCBI BLink). )

HSP 1 Score: 349.4 bits (895), Expect = 3.6e-96
Identity = 206/373 (55.23%), Postives = 264/373 (70.78%), Query Frame = 0

Query: 2   AASARAFF-LSRVTDFSIKPRLPPQPPPPPSLPSFSSPHLGVLRRRFTSSSGATTVSCLV 61
           AASARAFF LSRVTD S K  +  QPPP       SSPH     R   + + A + S ++
Sbjct: 3   AASARAFFMLSRVTDLSKKKLILHQPPPS------SSPH-----RLPYAPNRAVSSSAVI 62

Query: 62  SGVDGGGVS--DDFVSTRKLKFDRGFSVIANMLKRIEPLDTSDISKGVSDAAKDSMKQTI 121
           S + GGGVS  D +VSTR+ K DRGF+VIAN++ RI+PLDTS ISKG+SD+AKDSMKQTI
Sbjct: 63  SCLSGGGVSSDDSYVSTRRSKLDRGFAVIANLVNRIQPLDTSVISKGLSDSAKDSMKQTI 122

Query: 122 SSMLGLLPSDQFAVTVRVSKNPLHNILSSSIITGYTLWNAEYRLSLMRNFDISPDNLTGL 181
           SSMLGLLPSDQF+V+V +S+ PL+ +L SSIITGYTLWNAEYR+SL RNFDI  D     
Sbjct: 123 SSMLGLLPSDQFSVSVTISEQPLYRLLISSIITGYTLWNAEYRVSLRRNFDIPID----- 182

Query: 182 NRSKPMEVSDVEETLVGVDSD--------VEDLDRTRPRLLTDLPPEALKYIQQLQSELS 241
            R +  + S  +    G +          VE+ +R  P++  DL PEAL YIQ LQSELS
Sbjct: 183 PRKEEEDQSSKDNVRFGSEKGMSEDLGNCVEEFERLSPQVFGDLSPEALSYIQLLQSELS 242

Query: 242 NLKDELNTRKQENMQMEYGRGNRNNLLEYLRSLDSNMVTELCKPSTLEVEEIIHELVGNI 301
           ++K+EL+++K++ +++E  +GNRN+LL+YLRSLD  MVTEL + S+ EVEEI+++LV N+
Sbjct: 243 SMKEELDSQKKKALRIECEKGNRNDLLDYLRSLDPEMVTELSQLSSPEVEEIVNQLVQNV 302

Query: 302 LQRFFKDDASSTFVEDSSVADLGKLANAGDEFYDTVGTSRDYLAKLLFWCMLLGHHLRSL 361
           L+R F+D  +S F+++  +    +    GD     V TSRDYLAKLLFWCMLLGHHLR L
Sbjct: 303 LERLFEDQTTSNFMQNPGI----RTTEGGDGTGRKVDTSRDYLAKLLFWCMLLGHHLRGL 355

Query: 362 ENRLQLSCVVGLL 364
           ENRL LSCVVGLL
Sbjct: 363 ENRLHLSCVVGLL 355

BLAST of MS004716 vs. TAIR 10
Match: AT2G14910.1 (unknown protein; LOCATED IN: chloroplast; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G14970.1); Has 605 Blast hits to 425 proteins in 102 species: Archae - 0; Bacteria - 300; Metazoa - 25; Fungi - 0; Plants - 89; Viruses - 0; Other Eukaryotes - 191 (source: NCBI BLink). )

HSP 1 Score: 174.1 bits (440), Expect = 2.1e-43
Identity = 145/373 (38.87%), Postives = 205/373 (54.96%), Query Frame = 0

Query: 13  VTDFSIK-PRLPPQPPPPPSLP-SFSSPHLGVLRRRFTSSSGATTVSCLVSG--VDGGGV 72
           ++ FS+  P+L  +P  P  LP  F  P      R  T +S +TT S   S    D G  
Sbjct: 6   LSSFSLSLPQLLHKPTKP--LPFLFLLPRFNRRFRSLTITSSSTTSSNNFSSNCGDDGFS 65

Query: 73  SDDFVSTRKLKFDRGFSVIANMLKRIEPLDTSDISKGVSDAAKDSMKQTISSMLGLLPSD 132
            DDF      +  +   V++++++ IEPLD S I K V     D+MK+TIS MLGLLPSD
Sbjct: 66  LDDFTLHSDSRSPKK-CVLSDLIQEIEPLDVSLIQKDVPVTTLDAMKRTISGMLGLLPSD 125

Query: 133 QFAVTVRVSKNPLHNILSSSIITGYTLWNAEYRLSLMRNFDISP---DNLTGLNRSKPME 192
           +F V +     PL  +L SS++TGYTL NAEYRL L +N D+S    D+    N    ME
Sbjct: 126 RFQVHIESLWEPLSKLLVSSMMTGYTLRNAEYRLFLEKNLDMSGGGLDSHASENTEYDME 185

Query: 193 VSDVEETLVGV--DSDVEDLDRT-RPRLLTDLPPEALKYIQQLQSELSNLKDELNTRKQE 252
            +  +E  V    DS  ++L  T     L  +  EA +YI +LQS+LS++K EL   +++
Sbjct: 186 GTFPDEDHVSSKRDSRTQNLSETIDEEGLGRVSSEAQEYILRLQSQLSSVKKELQEMRRK 245

Query: 253 N--MQMEYGRG-NRNNLLEYLRSLDSNMVTELCKPSTLEVEEIIHELVGNIL-------- 312
           N  +QM+   G  +N+LL+YLRSL    V EL +P+  EV+E IH +V  +L        
Sbjct: 246 NAALQMQQFVGEEKNDLLDYLRSLQPEKVAELSEPAAPEVKETIHSVVHGLLATLSPKMH 305

Query: 313 QRFFKDDASST-FVEDSSVADLGKLA-NAGDEFYDTVGTSRDYLAKLLFWCMLLGHHLRS 363
            +F   +   T  V+  S  D  +L  N   +F   +  +RDYLA+LLFWCMLLGH+LR 
Sbjct: 306 SKFPASEVPPTETVKAKSDEDCAELVENTSLQFQPLISLTRDYLARLLFWCMLLGHYLRG 365

BLAST of MS004716 vs. TAIR 10
Match: AT2G14910.2 (unknown protein; LOCATED IN: chloroplast; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G14970.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 147.1 bits (370), Expect = 2.7e-35
Identity = 131/350 (37.43%), Postives = 187/350 (53.43%), Query Frame = 0

Query: 13  VTDFSIK-PRLPPQPPPPPSLP-SFSSPHLGVLRRRFTSSSGATTVSCLVSG--VDGGGV 72
           ++ FS+  P+L  +P  P  LP  F  P      R  T +S +TT S   S    D G  
Sbjct: 6   LSSFSLSLPQLLHKPTKP--LPFLFLLPRFNRRFRSLTITSSSTTSSNNFSSNCGDDGFS 65

Query: 73  SDDFVSTRKLKFDRGFSVIANMLKRIEPLDTSDISKGVSDAAKDSMKQTISSMLGLLPSD 132
            DDF      +  +   V++++++ IEPLD S I K V     D+MK+TIS MLGLLPSD
Sbjct: 66  LDDFTLHSDSRSPKK-CVLSDLIQEIEPLDVSLIQKDVPVTTLDAMKRTISGMLGLLPSD 125

Query: 133 QFAVTVRVSKNPLHNILSSSIITGYTLWNAEYRLSLMRNFDISP---DNLTGLNRSKPME 192
           +F V +     PL  +L SS++TGYTL NAEYRL L +N D+S    D+    N    ME
Sbjct: 126 RFQVHIESLWEPLSKLLVSSMMTGYTLRNAEYRLFLEKNLDMSGGGLDSHASENTEYDME 185

Query: 193 VSDVEETLVGV--DSDVEDLDRT-RPRLLTDLPPEALKYIQQLQSELSNLKDELNTRKQE 252
            +  +E  V    DS  ++L  T     L  +  EA +YI +LQS+LS++K EL   +++
Sbjct: 186 GTFPDEDHVSSKRDSRTQNLSETIDEEGLGRVSSEAQEYILRLQSQLSSVKKELQEMRRK 245

Query: 253 N--MQMEYGRG-NRNNLLEYLRSLDSNMVTELCKPSTLEVEEIIHELVGNIL-------- 312
           N  +QM+   G  +N+LL+YLRSL    V EL +P+  EV+E IH +V  +L        
Sbjct: 246 NAALQMQQFVGEEKNDLLDYLRSLQPEKVAELSEPAAPEVKETIHSVVHGLLATLSPKMH 305

Query: 313 QRFFKDDASST-FVEDSSVADLGKLA-NAGDEFYDTVGTSRDYLAKLLFW 340
            +F   +   T  V+  S  D  +L  N   +F   +  +RDYLA+LLFW
Sbjct: 306 SKFPASEVPPTETVKAKSDEDCAELVENTSLQFQPLISLTRDYLARLLFW 352

BLAST of MS004716 vs. TAIR 10
Match: AT1G63610.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G14910.1); Has 537 Blast hits to 411 proteins in 100 species: Archae - 0; Bacteria - 231; Metazoa - 0; Fungi - 0; Plants - 94; Viruses - 0; Other Eukaryotes - 212 (source: NCBI BLink). )

HSP 1 Score: 77.0 bits (188), Expect = 3.4e-14
Identity = 94/357 (26.33%), Postives = 153/357 (42.86%), Query Frame = 0

Query: 25  QPPPPPSLPSFSSPHLGVLRRRFTSSSGAT------------TVSCLVSGVDGGGVSDDF 84
           Q PP    PSF + H   L    T+SS  T            T + LV  V   G S D 
Sbjct: 12  QSPPSRPCPSFLANHEPKLS---TTSSSVTFPLKTNTWKCSGTGNLLVLRVKAYGSSSDS 71

Query: 85  VS--------TRKLKFDRGFSVIANMLKRIEPLDTSDISKGVSDAAKDSMKQTISSMLGL 144
            +        TR+ K  R   ++   ++ ++P       K       ++M+QT+++M+G 
Sbjct: 72  SADSSTPPNGTRQPKSRR--DILLEYVQNVKPEFMEMFVKRAPKHVVEAMRQTVTNMIGT 131

Query: 145 LPSDQFAVTVRVSKNPLHNILSSSIITGYTLWNAEYRLSLMRNFDISPDNLTGLNRSKPM 204
           LP   FAVTV      L  ++ S ++TGY   NA+YRL L ++ +        L   +  
Sbjct: 132 LPPQFFAVTVTSVAENLAQLMMSVLMTGYMFRNAQYRLELQQSLE-----QVALPEPRDQ 191

Query: 205 EVSDVEETLVGVDSDVEDLDRTRPRLLTDLPPEALKYIQQLQSELSNLKDELNTRKQENM 264
           +  D E+   G   +V         +      +A KYI+ L++E+  L  ++  RK  N 
Sbjct: 192 KGGD-EDYAPGTQKNVSGEVIRWNNVSGPEKIDAKKYIELLEAEIEELNRQVG-RKSANQ 251

Query: 265 QMEYGRGNRNNLLEYLRSLDSNMVTELCKPSTLEVEEIIHELVGNILQRFFKDDASSTFV 324
           Q        N +LEYL+SL+   + EL   +  +V   ++  V  +L      +   T V
Sbjct: 252 Q--------NEILEYLKSLEPQNLKELTSTAGEDVAVAMNTFVKRLL-AVSDPNQMKTNV 311

Query: 325 EDSSVADLGKLANAGDEFYDTVGTSRDYLAKLLFWCMLLGHHLRSLENRLQLSCVVG 362
            ++S AD                     LAKLL+W M++G+ +R++E R  +  V+G
Sbjct: 312 TETSAAD---------------------LAKLLYWLMVVGYSIRNIEVRFDMERVLG 326

BLAST of MS004716 vs. TAIR 10
Match: AT1G63610.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G14910.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 75.1 bits (183), Expect = 1.3e-13
Identity = 93/357 (26.05%), Postives = 152/357 (42.58%), Query Frame = 0

Query: 25  QPPPPPSLPSFSSPHLGVLRRRFTSSSGAT------------TVSCLVSGVDGGGVSDDF 84
           Q PP    PSF + H   L    T+SS  T            T + LV  V   G S D 
Sbjct: 12  QSPPSRPCPSFLANHEPKLS---TTSSSVTFPLKTNTWKCSGTGNLLVLRVKAYGSSSDS 71

Query: 85  VS--------TRKLKFDRGFSVIANMLKRIEPLDTSDISKGVSDAAKDSMKQTISSMLGL 144
            +        TR+    R   ++   ++ ++P       K       ++M+QT+++M+G 
Sbjct: 72  SADSSTPPNGTRQQPKSRR-DILLEYVQNVKPEFMEMFVKRAPKHVVEAMRQTVTNMIGT 131

Query: 145 LPSDQFAVTVRVSKNPLHNILSSSIITGYTLWNAEYRLSLMRNFDISPDNLTGLNRSKPM 204
           LP   FAVTV      L  ++ S ++TGY   NA+YRL L ++ +        L   +  
Sbjct: 132 LPPQFFAVTVTSVAENLAQLMMSVLMTGYMFRNAQYRLELQQSLE-----QVALPEPRDQ 191

Query: 205 EVSDVEETLVGVDSDVEDLDRTRPRLLTDLPPEALKYIQQLQSELSNLKDELNTRKQENM 264
           +  D E+   G   +V         +      +A KYI+ L++E+  L  ++  RK  N 
Sbjct: 192 KGGD-EDYAPGTQKNVSGEVIRWNNVSGPEKIDAKKYIELLEAEIEELNRQVG-RKSANQ 251

Query: 265 QMEYGRGNRNNLLEYLRSLDSNMVTELCKPSTLEVEEIIHELVGNILQRFFKDDASSTFV 324
           Q        N +LEYL+SL+   + EL   +  +V   ++  V  +L      +   T V
Sbjct: 252 Q--------NEILEYLKSLEPQNLKELTSTAGEDVAVAMNTFVKRLL-AVSDPNQMKTNV 311

Query: 325 EDSSVADLGKLANAGDEFYDTVGTSRDYLAKLLFWCMLLGHHLRSLENRLQLSCVVG 362
            ++S AD                     LAKLL+W M++G+ +R++E R  +  V+G
Sbjct: 312 TETSAAD---------------------LAKLLYWLMVVGYSIRNIEVRFDMERVLG 327