MS004557 (gene) Bitter gourd (TR) v1

Overview


Name	MS004557
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	RING-type E3 ubiquitin transferase
Location	scaffold995: 241247 .. 242227 (-)
RNA-Seq Expression	MS004557
Synteny	MS004557

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCACTGAGCCCACCTCGTGCAACAACCAACCCCAACATCAGGACCTTCGACCTCTACTGGTGCTACCAATGCCACCGCGCGGTCAGGCTCGCCGCCGCGCACTCCTCCGACCTCACCTGCCCGCGCTGTTTCGGGCACTTCCTCGAAGAGCTCCACGTCCCTCGCCCCACCCAGCTCGACCTCTCGCCGGAGGCCAGATTGCTCGAAGCCCTTTCGCTAATGCTCGACCCGCCGGTTCGGCTCGTGAATTTCAGACTCCCGGACCCGGAACATGAACCCCGCCGCCGTCCACTCTGGCAATTTTTCGACGATTTCGACAGGAGACCGCTTCGCGATCTGGAAGGAGACGATTCCCTCCGGTGGCGAACACGGCGCCGTAACCGGAGCCTGGATGAGAGGGAAAGTTCCGGCCTAGGACTAGAACCCCCAAATCGGAACCAGCCTCGAACTTTCATCGTTATCAGACCCATAGACCAATCTCGGCCGATGCAGCCCCTAATTCCACCGGGCGCCAATCCAAGAGACTACTTTGCCGGACCACGACTGGACGACTTAATCGAAGAACTGACGCAGAACGACCGGCCGGGGCCGCCGCCGGCGAGCGATGCGGCGATCGAGGGGATACCGACGGTGAAGATAGTGGCGGAGCATCTGAAGAACGATTCGCACTGTCCGGTTTGTAAGGAGGAGTTTGAGGTGGGAGGAGAAGCGAGGGAATTGAACTGCAAGCACATATACCATTCGGACTGCATCGTGCCATGGCTAAGGCTCCATAACTCTTGTCCGGTGTGCAGGCAAGAGATGCCGCCATTGCCGCCAGCGCCGGAGAATCGAGGACCGGGAAGTTCTCAGGACGGCGAAGGGATTGGGAGAAGATGTGCCCGTTGGTGGGGCCAGTTGGTTTCTAGGCCATTTCGAAATAGGTATCGCCAGATTACCCCTCACGACCAGGAGAACATTCCTCCTCGAGGTGATGCC

mRNA sequence

ATGTCACTGAGCCCACCTCGTGCAACAACCAACCCCAACATCAGGACCTTCGACCTCTACTGGTGCTACCAATGCCACCGCGCGGTCAGGCTCGCCGCCGCGCACTCCTCCGACCTCACCTGCCCGCGCTGTTTCGGGCACTTCCTCGAAGAGCTCCACGTCCCTCGCCCCACCCAGCTCGACCTCTCGCCGGAGGCCAGATTGCTCGAAGCCCTTTCGCTAATGCTCGACCCGCCGGTTCGGCTCGTGAATTTCAGACTCCCGGACCCGGAACATGAACCCCGCCGCCGTCCACTCTGGCAATTTTTCGACGATTTCGACAGGAGACCGCTTCGCGATCTGGAAGGAGACGATTCCCTCCGGTGGCGAACACGGCGCCGTAACCGGAGCCTGGATGAGAGGGAAAGTTCCGGCCTAGGACTAGAACCCCCAAATCGGAACCAGCCTCGAACTTTCATCGTTATCAGACCCATAGACCAATCTCGGCCGATGCAGCCCCTAATTCCACCGGGCGCCAATCCAAGAGACTACTTTGCCGGACCACGACTGGACGACTTAATCGAAGAACTGACGCAGAACGACCGGCCGGGGCCGCCGCCGGCGAGCGATGCGGCGATCGAGGGGATACCGACGGTGAAGATAGTGGCGGAGCATCTGAAGAACGATTCGCACTGTCCGGTTTGTAAGGAGGAGTTTGAGGTGGGAGGAGAAGCGAGGGAATTGAACTGCAAGCACATATACCATTCGGACTGCATCGTGCCATGGCTAAGGCTCCATAACTCTTGTCCGGTGTGCAGGCAAGAGATGCCGCCATTGCCGCCAGCGCCGGAGAATCGAGGACCGGGAAGTTCTCAGGACGGCGAAGGGATTGGGAGAAGATGTGCCCGTTGGTGGGGCCAGTTGGTTTCTAGGCCATTTCGAAATAGGTATCGCCAGATTACCCCTCACGACCAGGAGAACATTCCTCCTCGAGGTGATGCC

Coding sequence (CDS)

ATGTCACTGAGCCCACCTCGTGCAACAACCAACCCCAACATCAGGACCTTCGACCTCTACTGGTGCTACCAATGCCACCGCGCGGTCAGGCTCGCCGCCGCGCACTCCTCCGACCTCACCTGCCCGCGCTGTTTCGGGCACTTCCTCGAAGAGCTCCACGTCCCTCGCCCCACCCAGCTCGACCTCTCGCCGGAGGCCAGATTGCTCGAAGCCCTTTCGCTAATGCTCGACCCGCCGGTTCGGCTCGTGAATTTCAGACTCCCGGACCCGGAACATGAACCCCGCCGCCGTCCACTCTGGCAATTTTTCGACGATTTCGACAGGAGACCGCTTCGCGATCTGGAAGGAGACGATTCCCTCCGGTGGCGAACACGGCGCCGTAACCGGAGCCTGGATGAGAGGGAAAGTTCCGGCCTAGGACTAGAACCCCCAAATCGGAACCAGCCTCGAACTTTCATCGTTATCAGACCCATAGACCAATCTCGGCCGATGCAGCCCCTAATTCCACCGGGCGCCAATCCAAGAGACTACTTTGCCGGACCACGACTGGACGACTTAATCGAAGAACTGACGCAGAACGACCGGCCGGGGCCGCCGCCGGCGAGCGATGCGGCGATCGAGGGGATACCGACGGTGAAGATAGTGGCGGAGCATCTGAAGAACGATTCGCACTGTCCGGTTTGTAAGGAGGAGTTTGAGGTGGGAGGAGAAGCGAGGGAATTGAACTGCAAGCACATATACCATTCGGACTGCATCGTGCCATGGCTAAGGCTCCATAACTCTTGTCCGGTGTGCAGGCAAGAGATGCCGCCATTGCCGCCAGCGCCGGAGAATCGAGGACCGGGAAGTTCTCAGGACGGCGAAGGGATTGGGAGAAGATGTGCCCGTTGGTGGGGCCAGTTGGTTTCTAGGCCATTTCGAAATAGGTATCGCCAGATTACCCCTCACGACCAGGAGAACATTCCTCCTCGAGGTGATGCC

Protein sequence

MSLSPPRATTNPNIRTFDLYWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHVPRPTQLDLSPEARLLEALSLMLDPPVRLVNFRLPDPEHEPRRRPLWQFFDDFDRRPLRDLEGDDSLRWRTRRRNRSLDERESSGLGLEPPNRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYFAGPRLDDLIEELTQNDRPGPPPASDAAIEGIPTVKIVAEHLKNDSHCPVCKEEFEVGGEARELNCKHIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQDGEGIGRRCARWWGQLVSRPFRNRYRQITPHDQENIPPRGDA

Homology

[Close]

BLAST of MS004557 vs. NCBI nr
Match: KAG7024775.1 (E3 ubiquitin-protein ligase RZF1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 466.1 bits (1198), Expect = 2.5e-127
Identity = 228/322 (70.81%), Postives = 259/322 (80.43%), Query Frame = 0


Query: 1   MSLSPPRATTNP--NIRTFDLYWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHVPRPT 60
           M+LSPP  TTN   + RTF LYWC+QCHR V LA+A  SDLTCPRCFGHF+EE+H+P P 
Sbjct: 1   MALSPPPRTTNDRRHARTFHLYWCHQCHRTVTLASAEPSDLTCPRCFGHFVEEVHLPLP- 60

Query: 61  QLDLSPEARLLEALSLMLDPPVRLVNFRLPDPEHEPRRRPLWQFFDDFDRRPLRDLEGDD 120
           +LD SPE RLLEALSLMLDPPVRL N+R PDP+   RRRP    FDDFDRR   D EGD+
Sbjct: 61  ELDPSPETRLLEALSLMLDPPVRLFNYRHPDPD---RRRPPLHRFDDFDRRSFSDPEGDE 120

Query: 121 SLRWRTRRRNRSLDERESSGLGLEPPNRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYF 180
            L WRTRRR+RSLDER++S  G +PPN NQ RT IV+ P DQ +P+QP+IP  ANPRDYF
Sbjct: 121 WLHWRTRRRSRSLDERDNS--GRQPPNPNQSRTVIVLGPPDQFQPIQPIIPRRANPRDYF 180

Query: 181 AGPRLDDLIEELTQNDRPGPPPASDAAIEGIPTVKIVAEHLKNDSHCPVCKEEFEVGGEA 240
            GPRLD+LIE+LTQNDRPGPPPA + AIE IPTVKI AEHLKNDSHCPVCKEEFEVGGEA
Sbjct: 181 TGPRLDELIEDLTQNDRPGPPPAGEEAIERIPTVKIAAEHLKNDSHCPVCKEEFEVGGEA 240

Query: 241 RELNCKHIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQDGEGIGRRCARWW 300
           REL+CKH+YHS+CIVPWLRLHNSCPVCRQE+PPLP  PEN   G+S++G  IGR C RWW
Sbjct: 241 RELSCKHLYHSECIVPWLRLHNSCPVCRQEIPPLP--PENGAAGNSENGVRIGRACTRWW 300

Query: 301 GQLVSRPFRNRYRQITPHDQEN 321
            +L S  FRNRYRQI+P    N
Sbjct: 301 NRLASWLFRNRYRQISPSSPPN 314

[Close]

BLAST of MS004557 vs. NCBI nr
Match: KAG6591901.1 (E3 ubiquitin-protein ligase RZF1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 466.1 bits (1198), Expect = 2.5e-127
Identity = 228/322 (70.81%), Postives = 259/322 (80.43%), Query Frame = 0


Query: 1   MSLSPPRATTNP--NIRTFDLYWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHVPRPT 60
           M+LSPP  TTN   + RTF LYWC+QCHR V LA+A  SDLTCPRCFGHF+EE+H+P P 
Sbjct: 1   MALSPPPRTTNDRRHARTFHLYWCHQCHRTVTLASAEPSDLTCPRCFGHFVEEVHLPLP- 60

Query: 61  QLDLSPEARLLEALSLMLDPPVRLVNFRLPDPEHEPRRRPLWQFFDDFDRRPLRDLEGDD 120
           +LD SPE RLLEALSLMLDPPVRL N+R PDP+   RRRP    FDDFDRR   D EGD+
Sbjct: 61  ELDPSPETRLLEALSLMLDPPVRLFNYRHPDPD---RRRPPLHRFDDFDRRSFSDPEGDE 120

Query: 121 SLRWRTRRRNRSLDERESSGLGLEPPNRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYF 180
            L WRTRRR+RSLDER++S  G +PPN NQ RT IV+ P DQ +P+QP+IP  ANPRDYF
Sbjct: 121 WLHWRTRRRSRSLDERDNS--GRQPPNPNQSRTVIVLGPPDQFQPIQPIIPRRANPRDYF 180

Query: 181 AGPRLDDLIEELTQNDRPGPPPASDAAIEGIPTVKIVAEHLKNDSHCPVCKEEFEVGGEA 240
            GPRLD+LIE+LTQNDRPGPPPA + AIE IPTVKI AEHLKNDSHCPVCKEEFEVGGEA
Sbjct: 181 TGPRLDELIEDLTQNDRPGPPPAGEEAIERIPTVKIAAEHLKNDSHCPVCKEEFEVGGEA 240

Query: 241 RELNCKHIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQDGEGIGRRCARWW 300
           REL+CKH+YHS+CIVPWLRLHNSCPVCRQE+PPLP  PEN   G+S++G  IGR C RWW
Sbjct: 241 RELSCKHLYHSECIVPWLRLHNSCPVCRQEIPPLP--PENGAAGNSENGVRIGRACTRWW 300

Query: 301 GQLVSRPFRNRYRQITPHDQEN 321
            +L S  FRNRYRQI+P    N
Sbjct: 301 NRLASWLFRNRYRQISPSSPPN 314

[Close]

BLAST of MS004557 vs. NCBI nr
Match: XP_022975873.1 (E3 ubiquitin-protein ligase RZF1-like [Cucurbita maxima])

HSP 1 Score: 464.2 bits (1193), Expect = 9.6e-127
Identity = 229/322 (71.12%), Postives = 258/322 (80.12%), Query Frame = 0


Query: 1   MSLSPPRATTNP--NIRTFDLYWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHVPRPT 60
           M+LSPP  TTN   + RTF LYWC+QCHR V LA+A  SDLTCPRCFGHF+EE+H+P P 
Sbjct: 1   MALSPPPHTTNDRRHARTFHLYWCHQCHRTVTLASAEPSDLTCPRCFGHFVEEVHLPLP- 60

Query: 61  QLDLSPEARLLEALSLMLDPPVRLVNFRLPDPEHEPRRRPLWQFFDDFDRRPLRDLEGDD 120
           +LD SPE RLLEALSLMLDPPVRL N+R PDP+   RRRP    FDDFDRR   D EGD+
Sbjct: 61  ELDPSPETRLLEALSLMLDPPVRLFNYRHPDPD---RRRPPLHRFDDFDRRSFSDPEGDE 120

Query: 121 SLRWRTRRRNRSLDERESSGLGLEPPNRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYF 180
            L WRTRRR+RSLDER++S  G +PPN NQ RT IV+ P DQ +P+QP+IP  ANPRDYF
Sbjct: 121 WLHWRTRRRSRSLDERDNS--GRQPPNPNQSRTVIVLGPPDQFQPIQPIIPRRANPRDYF 180

Query: 181 AGPRLDDLIEELTQNDRPGPPPASDAAIEGIPTVKIVAEHLKNDSHCPVCKEEFEVGGEA 240
            GPRLD+LIE+LTQNDRPGPPPA + AIE IPTVKI AEHLKNDSHCPVCKEEFEVGGEA
Sbjct: 181 TGPRLDELIEDLTQNDRPGPPPAGEEAIERIPTVKIAAEHLKNDSHCPVCKEEFEVGGEA 240

Query: 241 RELNCKHIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQDGEGIGRRCARWW 300
           REL+CKHIYHS+CIVPWLRLHNSCPVCRQE+PPLP  PEN   G+S+    IGR C RWW
Sbjct: 241 RELSCKHIYHSECIVPWLRLHNSCPVCRQEIPPLP--PENGAAGNSETEVRIGRACTRWW 300

Query: 301 GQLVSRPFRNRYRQITPHDQEN 321
            +LVS  FRNRYRQI+P    N
Sbjct: 301 NRLVSWLFRNRYRQISPSSPPN 314

[Close]

BLAST of MS004557 vs. NCBI nr
Match: XP_022937137.1 (E3 ubiquitin-protein ligase RZF1-like [Cucurbita moschata] >XP_022937138.1 E3 ubiquitin-protein ligase RZF1-like [Cucurbita moschata])

HSP 1 Score: 463.0 bits (1190), Expect = 2.1e-126
Identity = 227/322 (70.50%), Postives = 258/322 (80.12%), Query Frame = 0


Query: 1   MSLSPPRATTNP--NIRTFDLYWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHVPRPT 60
           M+LSPP  TTN   + RTF LYWC+QCHR V LA+A  SDLTCPRCFGHF+EE+H+P P 
Sbjct: 1   MALSPPPRTTNDRRHARTFHLYWCHQCHRTVTLASAEPSDLTCPRCFGHFVEEVHLPLP- 60

Query: 61  QLDLSPEARLLEALSLMLDPPVRLVNFRLPDPEHEPRRRPLWQFFDDFDRRPLRDLEGDD 120
           +LD SPE RLLEALSLMLDPPVRL N+R PDP+   RRRP    FDDFDRR   D EGD+
Sbjct: 61  ELDPSPETRLLEALSLMLDPPVRLFNYRHPDPD---RRRPPLHRFDDFDRRSFSDPEGDE 120

Query: 121 SLRWRTRRRNRSLDERESSGLGLEPPNRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYF 180
            L WRTRRR+RSLDER++S  G +PPN NQ RT IV+ P DQ +P+QP+IP  ANPRDYF
Sbjct: 121 WLHWRTRRRSRSLDERDNS--GRQPPNPNQSRTVIVLGPPDQFQPIQPIIPRRANPRDYF 180

Query: 181 AGPRLDDLIEELTQNDRPGPPPASDAAIEGIPTVKIVAEHLKNDSHCPVCKEEFEVGGEA 240
            GPRLD+LIE+LTQNDRPGPPPA + AIE IPTVKI AEHLKNDS CPVCKEEFEVGGEA
Sbjct: 181 TGPRLDELIEDLTQNDRPGPPPAGEEAIERIPTVKIAAEHLKNDSQCPVCKEEFEVGGEA 240

Query: 241 RELNCKHIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQDGEGIGRRCARWW 300
           REL+CKH+YHS+CIVPWLRLHNSCPVCRQE+PPLP  PEN   G+S++G  IGR C RWW
Sbjct: 241 RELSCKHLYHSECIVPWLRLHNSCPVCRQEIPPLP--PENGAAGNSENGVRIGRACTRWW 300

Query: 301 GQLVSRPFRNRYRQITPHDQEN 321
            +L S  FRNRYRQI+P    N
Sbjct: 301 NRLASWLFRNRYRQISPSSPPN 314

[Close]

BLAST of MS004557 vs. NCBI nr
Match: XP_023535798.1 (E3 ubiquitin-protein ligase RZF1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 454.5 bits (1168), Expect = 7.6e-124
Identity = 224/322 (69.57%), Postives = 255/322 (79.19%), Query Frame = 0


Query: 1   MSLSPPRATTNP--NIRTFDLYWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHVPRPT 60
           MS +PP  TTN   + RTF LYWC+QCHR V LA+A  SDLTCPRCFGHF+EE+H+P P 
Sbjct: 1   MSFTPPPHTTNDRRHARTFHLYWCHQCHRTVTLASAQPSDLTCPRCFGHFVEEVHLPLP- 60

Query: 61  QLDLSPEARLLEALSLMLDPPVRLVNFRLPDPEHEPRRRPLWQFFDDFDRRPLRDLEGDD 120
           +LD SPE RLLEALSLMLDPPV L N+R P P+H   RRP    FDDFDRR   D EGD+
Sbjct: 61  ELDPSPETRLLEALSLMLDPPVCLFNYRHPVPDH---RRPPLNRFDDFDRRSFSDPEGDE 120

Query: 121 SLRWRTRRRNRSLDERESSGLGLEPPNRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYF 180
            L WRTRRR+RSLDER++S  G +PPN NQ RT IV+ P DQ +P+QP+IP  ANPRDYF
Sbjct: 121 WLHWRTRRRSRSLDERDNS--GRQPPNPNQSRTVIVLGPPDQFQPIQPIIPRRANPRDYF 180

Query: 181 AGPRLDDLIEELTQNDRPGPPPASDAAIEGIPTVKIVAEHLKNDSHCPVCKEEFEVGGEA 240
            GPRLD+LIE+LTQNDRPGP PA + AIE IPTVKI AEHLKNDS CPVCKEEFEVGGEA
Sbjct: 181 TGPRLDELIEDLTQNDRPGPAPAGEEAIERIPTVKIAAEHLKNDSQCPVCKEEFEVGGEA 240

Query: 241 RELNCKHIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQDGEGIGRRCARWW 300
           REL+CKH+YHS+CIVPWLRLHNSCPVCRQE+PPLP  PEN   G+S++G  IGR C RWW
Sbjct: 241 RELSCKHLYHSECIVPWLRLHNSCPVCRQEIPPLP--PENGAAGNSENGVRIGRACTRWW 300

Query: 301 GQLVSRPFRNRYRQITPHDQEN 321
            +LVS  FRNRYRQI+P    N
Sbjct: 301 NRLVSWLFRNRYRQISPSSPPN 314

[Close]

BLAST of MS004557 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 1.1e-37
Identity = 99/280 (35.36%), Postives = 146/280 (52.14%), Query Frame = 0


Query: 19  LYWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHVPRPTQ-LDLSPEARLLEALSLMLD 78
           +++CYQC++ V ++ + S+D  CP C   FLEE   P P Q L+ +P +   ++   M D
Sbjct: 20  MFFCYQCNQTVTISISSSADPFCPICNQGFLEEYEDPNPNQSLNFNPNSS--DSFFPMAD 79

Query: 79  PPVRLVNFRLPDPEHEPRRRPLWQFFDDFDRRPLRDLEGD---DSLRWRTRRRNRSLDER 138
           P   L+          P        F    +   R  + +   D+    T  +N  L   
Sbjct: 80  PFSTLLPLIFGSSAAAPSGMDFMSLFGPSMQPQARSTQQNPQSDAFDPFTFLQNH-LQTL 139

Query: 139 ESSGLGLEPPNRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYFAGPRLDDLIEELTQND 198
            SSG   E         F++     ++ P  P      N  DYF GP L+ LI++L +ND
Sbjct: 140 RSSGTHFE---------FVI-----ENHPSDPGNRMPGNFGDYFFGPGLEQLIQQLAEND 199

Query: 199 --RPGPPPASDAAIEGIPTVKIVAEHLKND-SHCPVCKEEFEVGGEARELNCKHIYHSDC 258
             R G PPAS +AI+ +PTVK+  + LK++ + C VC +EFE G + +++ CKH++H DC
Sbjct: 200 PNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDC 259

Query: 259 IVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQDGEGIG 292
           ++PWL LHNSCPVCR E+P   P  ENR  GS   G+G G
Sbjct: 260 LLPWLELHNSCPVCRFELPTDDPDYENRSQGSQGSGDGQG 282

[Close]

BLAST of MS004557 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 6.2e-36
Identity = 102/282 (36.17%), Postives = 140/282 (49.65%), Query Frame = 0


Query: 20  YWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHVPRPTQLDLSPEAR----------LL 79
           +WC++C RAVRL   H  +  C  C G F+EEL + + +  D+    R          L+
Sbjct: 8   HWCHRCQRAVRL---HGQEPVCFYCGGGFVEELDMAQASPFDMFRSHRGVVERDQTFDLM 67

Query: 80  EALSLMLDPPVRLVNFRLPDPEH--EPRRRPLWQFFDDF-DRRPLRDLEGDDSLRWRTRR 139
           +A S+ +         RL +  H  E R R +    ++F    PL    G    R     
Sbjct: 68  DAFSVFM-------RNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDN 127

Query: 140 RNRSLDERESSGLGLEPPNRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYFAGPRLDDL 199
              +L    S G+G+                               N  DYF GP L++L
Sbjct: 128 AVEALFNGGSPGIGI----------------------------TRGNTGDYFFGPGLEEL 187

Query: 200 IEELTQ-NDRPGPPPASDAAIEGIPTVKIVAEHLK-NDSHCPVCKEEFEVGGEARELNCK 259
            E+L+    R GPPPA  +AI+ +PT+KI   HL+ +DS+CPVCK+EFE+G EA+++ C 
Sbjct: 188 FEQLSAGTTRRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCN 245

Query: 260 HIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQD 287
           HIYHSDCIVPWL  HNSCPVCRQE+      P   GP SSQ+
Sbjct: 248 HIYHSDCIVPWLVQHNSCPVCRQEL------PSASGPSSSQN 245

[Close]

BLAST of MS004557 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 4.0e-35
Identity = 106/297 (35.69%), Postives = 143/297 (48.15%), Query Frame = 0


Query: 20  YWCYQCHRAVRLA-AAHSSDLTCPRCFGHFLEELHVPRPTQ----LDLSPEARLLEALSL 79
           ++CYQC+R V +  +  SSD TCP C   FLEE   P P Q    L+ +P +     L L
Sbjct: 23  FFCYQCNRTVNVTISPPSSDPTCPICNEGFLEEYDNPNPNQGSGFLNPNPNSIPFHDLFL 82

Query: 80  ML-DPPVRLVNFRLPDPEH---------EPRRRPLWQFFDDFDRRPLRDLEGDDSLRWRT 139
            L DP   L+    P             +P    L          P R   GD       
Sbjct: 83  TLSDPFASLLPLLFPSSSSTTTSSSASIDPNNPSL--------SGPTRSGRGDPFAFDPF 142

Query: 140 RRRNRSLDERESSGLGLEPPNRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYFAGPRLD 199
                 L++  SSG  +E         F++     Q+ P        AN  DYF GP L+
Sbjct: 143 TFIQNHLNDLRSSGAQIE---------FVI-----QNNPSDQGFRLPANIGDYFIGPGLE 202

Query: 200 DLIEELTQND--RPGPPPASDAAIEGIPTVKIVAEHLKND-SHCPVCKEEFEVGGEAREL 259
            LI++L +ND  R G PPAS +AIE +P V I   +L ++ + C VC ++FE G EA+++
Sbjct: 203 QLIQQLAENDPNRYGTPPASKSAIEALPLVNITKSNLNSEFNQCAVCMDDFEEGTEAKQM 262

Query: 260 NCKHIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENR-----GPGSSQDGEGIGRR 294
            CKH+YH DC++PWL LHNSCPVCR E+P   P  E R     G     DG+  G+R
Sbjct: 263 PCKHLYHKDCLLPWLELHNSCPVCRHELPTDDPDYERRVRGAQGTSGGNDGDNSGQR 297

[Close]

BLAST of MS004557 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 9.9e-34
Identity = 105/290 (36.21%), Postives = 142/290 (48.97%), Query Frame = 0


Query: 20  YWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHV-PRPTQLDL--SPEARLLEALSLML 79
           +WC++C RAV L A    D  C  C G F+EE+ + P     D+   P   L+EA S  +
Sbjct: 8   HWCHRCQRAVWLRA---RDAVCSYCGGGFVEEIDIGPSRAHRDVERDPTFDLMEAFSAFM 67

Query: 80  DPPV------RLVNFRLPDPEHE--PRRRPLWQFFDDFDRRPLRDLEGDDSLRWRTRRRN 139
              +      R ++ RL     E      PL  F     + P R   GD+S         
Sbjct: 68  RSRLAERSYDREISGRLGSAGSESFSNLAPLLIF---GGQAPFRLAGGDNS-------SV 127

Query: 140 RSLDERESSGLGLEPPNRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYFAGPRLDDLIE 199
            +     + G+G                           I  G N  DYF GP L++LIE
Sbjct: 128 EAFVNGAAPGIG---------------------------IARGTNAGDYFFGPGLEELIE 187

Query: 200 ELTQ-NDRPGPPPASDAAIEGIPTVKIVAEHLK-NDSHCPVCKEEFEVGGEARELNCKHI 259
           +L+      GPPPA  ++I+ +PT+KI  +HLK +DSHCPVCK+EFE+  EA+++ C HI
Sbjct: 188 QLSSGTHHRGPPPAPKSSIDALPTIKITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHI 247

Query: 260 YHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQDGEGIGRRCAR 297
           YHSDCIVPWL  HNSCPVCR+E   LP    +    SSQ+    GR  +R
Sbjct: 248 YHSDCIVPWLVQHNSCPVCRKE---LPSRGSSSSTQSSQNRSTNGRENSR 254

[Close]

BLAST of MS004557 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 7.6e-26
Identity = 94/311 (30.23%), Postives = 137/311 (44.05%), Query Frame = 0


Query: 1   MSLSPPRATTNPNIRTFDLYWCYQCHRAVRLAAAHSS----DLTCPRCFGHFLEELHVPR 60
           M  +P   TT   I     YWCY C R V + A   +     + CP C G F+E+++   
Sbjct: 1   MPSTPTTPTTTTPITA--SYWCYSCTRFVSVWADQGTTTVGSVACPHCDGGFIEQIN--- 60

Query: 61  PTQLDLSPEARLLEALSLMLDPPVRLVNFRLPDPEHEPR--RRPLWQFFDDFDRRPLRDL 120
               D S  A     L++     VR +N          R  RRP +         P+  L
Sbjct: 61  ----DSSSAA---TELTIPASTEVRSINNNRRSVIRRRRSGRRPSF--------NPVIVL 120

Query: 121 EGDDSLRWRTRRRNRSLDER-------ESSGLGLEPPNRNQPRTFIVIRPIDQSRPMQPL 180
           +G    R      + + D R       + SG GL P                        
Sbjct: 121 QGGAGEREEGEEGDAARDRRAFEFYYDDGSGSGLRP------------------------ 180

Query: 181 IPPGANPRDYFAGPRLDDLIEELTQND-------RPGPPPASDAAIEGIPTVKIVAEHLK 240
           +P   +  +   G   + L+E+L+Q +       R G PPAS +AIE +P V+I   H+ 
Sbjct: 181 LPDSVS--EILMGSGFERLLEQLSQIEASATGIGRSGNPPASKSAIESLPRVEISDCHIG 240

Query: 241 NDSHCPVCKEEFEVGGEARELNCKHIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRG 292
           ++++C VC E FE   EARE+ CKH++H DCIVPWL + NSCPVCR E+P  P    NR 
Sbjct: 241 SEANCAVCTEIFETETEAREMPCKHLFHDDCIVPWLSIRNSCPVCRFELPSEP----NRR 261

[Close]

BLAST of MS004557 vs. ExPASy TrEMBL
Match: A0A6J1IKH7 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111476446 PE=4 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 4.7e-127
Identity = 229/322 (71.12%), Postives = 258/322 (80.12%), Query Frame = 0


Query: 1   MSLSPPRATTNP--NIRTFDLYWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHVPRPT 60
           M+LSPP  TTN   + RTF LYWC+QCHR V LA+A  SDLTCPRCFGHF+EE+H+P P 
Sbjct: 1   MALSPPPHTTNDRRHARTFHLYWCHQCHRTVTLASAEPSDLTCPRCFGHFVEEVHLPLP- 60

Query: 61  QLDLSPEARLLEALSLMLDPPVRLVNFRLPDPEHEPRRRPLWQFFDDFDRRPLRDLEGDD 120
           +LD SPE RLLEALSLMLDPPVRL N+R PDP+   RRRP    FDDFDRR   D EGD+
Sbjct: 61  ELDPSPETRLLEALSLMLDPPVRLFNYRHPDPD---RRRPPLHRFDDFDRRSFSDPEGDE 120

Query: 121 SLRWRTRRRNRSLDERESSGLGLEPPNRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYF 180
            L WRTRRR+RSLDER++S  G +PPN NQ RT IV+ P DQ +P+QP+IP  ANPRDYF
Sbjct: 121 WLHWRTRRRSRSLDERDNS--GRQPPNPNQSRTVIVLGPPDQFQPIQPIIPRRANPRDYF 180

Query: 181 AGPRLDDLIEELTQNDRPGPPPASDAAIEGIPTVKIVAEHLKNDSHCPVCKEEFEVGGEA 240
            GPRLD+LIE+LTQNDRPGPPPA + AIE IPTVKI AEHLKNDSHCPVCKEEFEVGGEA
Sbjct: 181 TGPRLDELIEDLTQNDRPGPPPAGEEAIERIPTVKIAAEHLKNDSHCPVCKEEFEVGGEA 240

Query: 241 RELNCKHIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQDGEGIGRRCARWW 300
           REL+CKHIYHS+CIVPWLRLHNSCPVCRQE+PPLP  PEN   G+S+    IGR C RWW
Sbjct: 241 RELSCKHIYHSECIVPWLRLHNSCPVCRQEIPPLP--PENGAAGNSETEVRIGRACTRWW 300

Query: 301 GQLVSRPFRNRYRQITPHDQEN 321
            +LVS  FRNRYRQI+P    N
Sbjct: 301 NRLVSWLFRNRYRQISPSSPPN 314

[Close]

BLAST of MS004557 vs. ExPASy TrEMBL
Match: A0A6J1FAA6 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111443524 PE=4 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 1.0e-126
Identity = 227/322 (70.50%), Postives = 258/322 (80.12%), Query Frame = 0


Query: 1   MSLSPPRATTNP--NIRTFDLYWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHVPRPT 60
           M+LSPP  TTN   + RTF LYWC+QCHR V LA+A  SDLTCPRCFGHF+EE+H+P P 
Sbjct: 1   MALSPPPRTTNDRRHARTFHLYWCHQCHRTVTLASAEPSDLTCPRCFGHFVEEVHLPLP- 60

Query: 61  QLDLSPEARLLEALSLMLDPPVRLVNFRLPDPEHEPRRRPLWQFFDDFDRRPLRDLEGDD 120
           +LD SPE RLLEALSLMLDPPVRL N+R PDP+   RRRP    FDDFDRR   D EGD+
Sbjct: 61  ELDPSPETRLLEALSLMLDPPVRLFNYRHPDPD---RRRPPLHRFDDFDRRSFSDPEGDE 120

Query: 121 SLRWRTRRRNRSLDERESSGLGLEPPNRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYF 180
            L WRTRRR+RSLDER++S  G +PPN NQ RT IV+ P DQ +P+QP+IP  ANPRDYF
Sbjct: 121 WLHWRTRRRSRSLDERDNS--GRQPPNPNQSRTVIVLGPPDQFQPIQPIIPRRANPRDYF 180

Query: 181 AGPRLDDLIEELTQNDRPGPPPASDAAIEGIPTVKIVAEHLKNDSHCPVCKEEFEVGGEA 240
            GPRLD+LIE+LTQNDRPGPPPA + AIE IPTVKI AEHLKNDS CPVCKEEFEVGGEA
Sbjct: 181 TGPRLDELIEDLTQNDRPGPPPAGEEAIERIPTVKIAAEHLKNDSQCPVCKEEFEVGGEA 240

Query: 241 RELNCKHIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQDGEGIGRRCARWW 300
           REL+CKH+YHS+CIVPWLRLHNSCPVCRQE+PPLP  PEN   G+S++G  IGR C RWW
Sbjct: 241 RELSCKHLYHSECIVPWLRLHNSCPVCRQEIPPLP--PENGAAGNSENGVRIGRACTRWW 300

Query: 301 GQLVSRPFRNRYRQITPHDQEN 321
            +L S  FRNRYRQI+P    N
Sbjct: 301 NRLASWLFRNRYRQISPSSPPN 314

[Close]

BLAST of MS004557 vs. ExPASy TrEMBL
Match: A0A6J1FTR5 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111446755 PE=4 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 5.2e-118
Identity = 220/326 (67.48%), Postives = 252/326 (77.30%), Query Frame = 0


Query: 1   MSLSPPRATTNPNIRTFDLYWCYQCHRAVRLAAAHS-SDLTCPRCFGHFLEELHVPRPTQ 60
           MS +  R T +    +F  YWC+QCHR V LA     S+L C RC G FLEELH+ RP Q
Sbjct: 1   MSFASSRHTRS----SFHFYWCHQCHRMVTLATTRDPSELICSRCSGQFLEELHLTRP-Q 60

Query: 61  LDLSPEARLLEALSLMLDPPVRLVNFRLPDPEHEPRRRPLWQFFDDFDRRPLRDLEGDDS 120
            D SPE RLLEALSLMLDPPVRL  FRLPD +   RRRP+   FD+FDR+   D EGD+ 
Sbjct: 61  FDNSPETRLLEALSLMLDPPVRL--FRLPDID---RRRPMLHQFDNFDRQSFSDPEGDEW 120

Query: 121 LRWRTRRRNRSLDERESSGLGLEPPNRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYFA 180
             WRTRRRNRSLDER++S   L+PPN+N+ RT I++RP D SRP+QP+IPP A+PRDYF 
Sbjct: 121 FHWRTRRRNRSLDERDNS--SLQPPNQNRSRTVIILRPTDPSRPVQPIIPPRADPRDYFT 180

Query: 181 GPRLDDLIEELTQNDRPGPPPASDAAIEGIPTVKIVAEHLKNDSHCPVCKEEFEVGGEAR 240
           GPRLD+LIEELTQNDRPGP PAS+AAIE IPTVKI AEHLKND+ CPVCKEEFE+G EAR
Sbjct: 181 GPRLDELIEELTQNDRPGPQPASEAAIERIPTVKIAAEHLKNDTMCPVCKEEFEIGEEAR 240

Query: 241 ELNCKHIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQDGEGIGRRCARWWG 300
           EL CKH+YHS+CIVPWLRLHNSCPVCRQEMPP P  PEN+   SS D EGIGRRC RWWG
Sbjct: 241 ELMCKHVYHSECIVPWLRLHNSCPVCRQEMPPSP--PENQAAESSDDEEGIGRRCTRWWG 300

Query: 301 QLVSRPFRNRYRQIT-PHDQENIPPR 325
           QLV+RPFR+RYRQ++ P D  N   R
Sbjct: 301 QLVNRPFRSRYRQLSPPQDSHNSSSR 312

[Close]

BLAST of MS004557 vs. ExPASy TrEMBL
Match: A0A6J1ISV3 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111480302 PE=4 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 1.7e-116
Identity = 218/311 (70.10%), Postives = 247/311 (79.42%), Query Frame = 0


Query: 16  TFDLYWCYQCHRAVRLA-AAHSSDLTCPRCFGHFLEELHVPRPTQLDLSPEARLLEALSL 75
           +F LYWC+QCHR V LA A   S+L C RC G FLEELH+ RP Q D SPE RLLEALSL
Sbjct: 12  SFHLYWCHQCHRMVTLATAGDPSELICSRCSGQFLEELHLTRP-QFDNSPETRLLEALSL 71

Query: 76  MLDPPVRLVNFRLPDPEHEPRRRPLWQFFDDFDRRPLRDLEGDDSLRWRTRRRNRSLDER 135
           MLDPPVRL  FRLPD +   RRRP+   FD+FDRR   D EGD+    RTRRRNRSLDER
Sbjct: 72  MLDPPVRL--FRLPDLD---RRRPMSHQFDNFDRRSFSDPEGDEWFHGRTRRRNRSLDER 131

Query: 136 ESSGLGLEPPNRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYFAGPRLDDLIEELTQND 195
           ++S   L PPN+N+ RTFI++RP D SRP+Q +IPP A+P DYF GPRLD+LIEELTQND
Sbjct: 132 DNS--SLLPPNQNRSRTFIILRPTDPSRPVQTIIPPRADPGDYFTGPRLDELIEELTQND 191

Query: 196 RPGPPPASDAAIEGIPTVKIVAEHLKNDSHCPVCKEEFEVGGEARELNCKHIYHSDCIVP 255
           RPGP PAS+AAIE IPTVKI AEHLKNDS CPVCKEEFE+G EAREL CKH+YHS+CIVP
Sbjct: 192 RPGPQPASEAAIERIPTVKIAAEHLKNDSMCPVCKEEFEIGEEARELMCKHVYHSECIVP 251

Query: 256 WLRLHNSCPVCRQEMPPLPPAPENRGPGSSQDGEGIGRRCARWWGQLVSRPFRNRYRQIT 315
           WLRLHNSCPVCRQEMPP P  PEN+   SS+D EGIGRRC RWWGQLV+RPFR+RYRQ++
Sbjct: 252 WLRLHNSCPVCRQEMPPSP--PENQAAESSEDEEGIGRRCTRWWGQLVNRPFRSRYRQLS 311

Query: 316 -PHDQENIPPR 325
            P D+ N   R
Sbjct: 312 PPQDRHNSSSR 312

[Close]

BLAST of MS004557 vs. ExPASy TrEMBL
Match: A0A5A7TUM6 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1493G00540 PE=4 SV=1)

HSP 1 Score: 417.5 bits (1072), Expect = 5.0e-113
Identity = 209/330 (63.33%), Postives = 251/330 (76.06%), Query Frame = 0


Query: 1   MSLSPPRATT-NPNIRTFDLYWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHVPRPTQ 60
           MSL+PP++TT N +  TF LYWC+QCHR + LA+AH+S+L CPRCFG F+EE+ +  P +
Sbjct: 1   MSLTPPQSTTNNRHTTTFQLYWCHQCHRTITLASAHTSELICPRCFGQFIEEIQLTLP-E 60

Query: 61  LDLSPEARLLEALSLMLDPPVRLVNFRLPDPEHEPRRRPLWQFFDDFDRRPLRDLEGDDS 120
            +  PEARL EALSLML+ P+RL N R P+     R  P    F++FDRR   D EGD+ 
Sbjct: 61  FNPLPEARLFEALSLMLNQPIRLFNNRTPNGN---RHHPPLHRFEEFDRRSFSDPEGDEL 120

Query: 121 LRWRTRRRNRSLDERESSGLGLEPPNRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYFA 180
            +WR R R+RSLDER++   G +PPN NQ RT IV  P DQ +P+QP++P   +PRDYF 
Sbjct: 121 PQWRRRWRSRSLDERDN--FGPQPPNPNQSRTVIVFGPPDQLQPIQPILPRRVSPRDYFT 180

Query: 181 GPRLDDLIEELTQNDRPGPPPASDAAIEGIPTVKIVAEHLKNDSHCPVCKEEFEVGGEAR 240
           GP+LD+LIEELTQNDRPGP PAS+ AIE IP VKI AEHLKNDSHCPVCKEEFEVGGEAR
Sbjct: 181 GPQLDELIEELTQNDRPGPAPASEEAIERIPMVKIAAEHLKNDSHCPVCKEEFEVGGEAR 240

Query: 241 ELNCKHIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQDGEGIGRRCARWWG 300
           EL+CKHIYHS+CIVPWLRLHNSCPVCRQEMP L   PEN    SS++ EG+GRRCARWW 
Sbjct: 241 ELSCKHIYHSECIVPWLRLHNSCPVCRQEMPSL--TPENEASSSSEE-EGMGRRCARWWS 300

Query: 301 QLVSRPFRNRYRQIT--PHDQENIPPRGDA 328
           QLV  PFRNRYRQI+  P ++ +   RGDA
Sbjct: 301 QLVPWPFRNRYRQISPFPRNRFDASSRGDA 321

[Close]

BLAST of MS004557 vs. TAIR 10
Match: AT1G60360.1 (RING/U-box superfamily protein )

HSP 1 Score: 238.8 bits (608), Expect = 6.1e-63
Identity = 147/347 (42.36%), Postives = 193/347 (55.62%), Query Frame = 0


Query: 1   MSLSPP--RATTNPN--IRTFDLYWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHV-- 60
           MSLS P  R  + PN   RTF LYWCY C R VR+A+++ S++ CPRC   F+ E+    
Sbjct: 1   MSLSQPITRTDSAPNGAFRTFGLYWCYHCDRMVRIASSNPSEIACPRCLRQFVVEIETRQ 60

Query: 61  -PRPT------QLDLSPEARLLEALSLMLDPPVRLVNFRLPDPEHEPRRRPLWQF-FDDF 120
            PR T        D SPEARLLEALSLM +P                    + +F  D F
Sbjct: 61  RPRFTFNHATPPFDASPEARLLEALSLMFEPAT------------------IGRFGADPF 120

Query: 121 DRRPLRDLEGDDSLRWRTRRRNRSLDERESSGLGLEPPNRNQPRTFIVIRPIDQSRPMQP 180
            R   R++   +S      RR  SLD   + GL L        RT++++RP + + P+  
Sbjct: 121 LRARSRNILEPESRPRPQHRRRHSLDNVNNGGLPL------PRRTYVILRPNNPTSPLGN 180

Query: 181 LIPP-------GANPRDYFAG-PRLDDLIEELTQNDRPGPPPASDAAIEGIPTVKIVAEH 240
           +I P         N  DYF G   L+ LIE+LTQ+DRPGPPPAS+  I  +P+VKI  +H
Sbjct: 181 IIAPPNQAPPRHVNSHDYFTGASSLEQLIEQLTQDDRPGPPPASEPTINSLPSVKITPQH 240

Query: 241 LKND-SHCPVCKEEFEVGGEARELNCKHIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPE 300
           L ND S C VC EEF VGG+A EL CKHIYH DCIVPWLRL+NSCP+CR+++P +    E
Sbjct: 241 LTNDMSQCTVCMEEFIVGGDATELPCKHIYHKDCIVPWLRLNNSCPICRRDLPLVNTVAE 300

Query: 301 NRGPGSSQDGEGIGRRCARWWGQLVSRPFRNRYRQITPHDQENIPPR 325
           +R   +    +   RR  RW       PFR RY++++P +  N  PR
Sbjct: 301 SRERSNPIRQDMPERRRPRWMQLGNIWPFRARYQRVSPEETANQNPR 323

[Close]

BLAST of MS004557 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 158.7 bits (400), Expect = 8.0e-39
Identity = 99/280 (35.36%), Postives = 146/280 (52.14%), Query Frame = 0


Query: 19  LYWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHVPRPTQ-LDLSPEARLLEALSLMLD 78
           +++CYQC++ V ++ + S+D  CP C   FLEE   P P Q L+ +P +   ++   M D
Sbjct: 20  MFFCYQCNQTVTISISSSADPFCPICNQGFLEEYEDPNPNQSLNFNPNSS--DSFFPMAD 79

Query: 79  PPVRLVNFRLPDPEHEPRRRPLWQFFDDFDRRPLRDLEGD---DSLRWRTRRRNRSLDER 138
           P   L+          P        F    +   R  + +   D+    T  +N  L   
Sbjct: 80  PFSTLLPLIFGSSAAAPSGMDFMSLFGPSMQPQARSTQQNPQSDAFDPFTFLQNH-LQTL 139

Query: 139 ESSGLGLEPPNRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYFAGPRLDDLIEELTQND 198
            SSG   E         F++     ++ P  P      N  DYF GP L+ LI++L +ND
Sbjct: 140 RSSGTHFE---------FVI-----ENHPSDPGNRMPGNFGDYFFGPGLEQLIQQLAEND 199

Query: 199 --RPGPPPASDAAIEGIPTVKIVAEHLKND-SHCPVCKEEFEVGGEARELNCKHIYHSDC 258
             R G PPAS +AI+ +PTVK+  + LK++ + C VC +EFE G + +++ CKH++H DC
Sbjct: 200 PNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDC 259

Query: 259 IVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQDGEGIG 292
           ++PWL LHNSCPVCR E+P   P  ENR  GS   G+G G
Sbjct: 260 LLPWLELHNSCPVCRFELPTDDPDYENRSQGSQGSGDGQG 282

[Close]

BLAST of MS004557 vs. TAIR 10
Match: AT2G40830.2 (RING-H2 finger C1A )

HSP 1 Score: 152.9 bits (385), Expect = 4.4e-37
Identity = 102/282 (36.17%), Postives = 140/282 (49.65%), Query Frame = 0


Query: 20  YWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHVPRPTQLDLSPEAR----------LL 79
           +WC++C RAVRL   H  +  C  C G F+EEL + + +  D+    R          L+
Sbjct: 8   HWCHRCQRAVRL---HGQEPVCFYCGGGFVEELDMAQASPFDMFRSHRGVVERDQTFDLM 67

Query: 80  EALSLMLDPPVRLVNFRLPDPEH--EPRRRPLWQFFDDF-DRRPLRDLEGDDSLRWRTRR 139
           +A S+ +         RL +  H  E R R +    ++F    PL    G    R     
Sbjct: 68  DAFSVFM-------RNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDN 127

Query: 140 RNRSLDERESSGLGLEPPNRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYFAGPRLDDL 199
              +L    S G+G+                               N  DYF GP L++L
Sbjct: 128 AVEALFNGGSPGIGI----------------------------TRGNTGDYFFGPGLEEL 187

Query: 200 IEELTQ-NDRPGPPPASDAAIEGIPTVKIVAEHLK-NDSHCPVCKEEFEVGGEARELNCK 259
            E+L+    R GPPPA  +AI+ +PT+KI   HL+ +DS+CPVCK+EFE+G EA+++ C 
Sbjct: 188 FEQLSAGTTRRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCN 245

Query: 260 HIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQD 287
           HIYHSDCIVPWL  HNSCPVCRQE+      P   GP SSQ+
Sbjct: 248 HIYHSDCIVPWLVQHNSCPVCRQEL------PSASGPSSSQN 245

[Close]

BLAST of MS004557 vs. TAIR 10
Match: AT2G40830.1 (RING-H2 finger C1A )

HSP 1 Score: 152.9 bits (385), Expect = 4.4e-37
Identity = 102/282 (36.17%), Postives = 140/282 (49.65%), Query Frame = 0


Query: 20  YWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHVPRPTQLDLSPEAR----------LL 79
           +WC++C RAVRL   H  +  C  C G F+EEL + + +  D+    R          L+
Sbjct: 8   HWCHRCQRAVRL---HGQEPVCFYCGGGFVEELDMAQASPFDMFRSHRGVVERDQTFDLM 67

Query: 80  EALSLMLDPPVRLVNFRLPDPEH--EPRRRPLWQFFDDF-DRRPLRDLEGDDSLRWRTRR 139
           +A S+ +         RL +  H  E R R +    ++F    PL    G    R     
Sbjct: 68  DAFSVFM-------RNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDN 127

Query: 140 RNRSLDERESSGLGLEPPNRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYFAGPRLDDL 199
              +L    S G+G+                               N  DYF GP L++L
Sbjct: 128 AVEALFNGGSPGIGI----------------------------TRGNTGDYFFGPGLEEL 187

Query: 200 IEELTQ-NDRPGPPPASDAAIEGIPTVKIVAEHLK-NDSHCPVCKEEFEVGGEARELNCK 259
            E+L+    R GPPPA  +AI+ +PT+KI   HL+ +DS+CPVCK+EFE+G EA+++ C 
Sbjct: 188 FEQLSAGTTRRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCN 245

Query: 260 HIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQD 287
           HIYHSDCIVPWL  HNSCPVCRQE+      P   GP SSQ+
Sbjct: 248 HIYHSDCIVPWLVQHNSCPVCRQEL------PSASGPSSSQN 245

[Close]

BLAST of MS004557 vs. TAIR 10
Match: AT2G40830.3 (RING-H2 finger C1A )

HSP 1 Score: 152.9 bits (385), Expect = 4.4e-37
Identity = 102/282 (36.17%), Postives = 140/282 (49.65%), Query Frame = 0


Query: 20  YWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHVPRPTQLDLSPEAR----------LL 79
           +WC++C RAVRL   H  +  C  C G F+EEL + + +  D+    R          L+
Sbjct: 8   HWCHRCQRAVRL---HGQEPVCFYCGGGFVEELDMAQASPFDMFRSHRGVVERDQTFDLM 67

Query: 80  EALSLMLDPPVRLVNFRLPDPEH--EPRRRPLWQFFDDF-DRRPLRDLEGDDSLRWRTRR 139
           +A S+ +         RL +  H  E R R +    ++F    PL    G    R     
Sbjct: 68  DAFSVFM-------RNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDN 127

Query: 140 RNRSLDERESSGLGLEPPNRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYFAGPRLDDL 199
              +L    S G+G+                               N  DYF GP L++L
Sbjct: 128 AVEALFNGGSPGIGI----------------------------TRGNTGDYFFGPGLEEL 187

Query: 200 IEELTQ-NDRPGPPPASDAAIEGIPTVKIVAEHLK-NDSHCPVCKEEFEVGGEARELNCK 259
            E+L+    R GPPPA  +AI+ +PT+KI   HL+ +DS+CPVCK+EFE+G EA+++ C 
Sbjct: 188 FEQLSAGTTRRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCN 245

Query: 260 HIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQD 287
           HIYHSDCIVPWL  HNSCPVCRQE+      P   GP SSQ+
Sbjct: 248 HIYHSDCIVPWLVQHNSCPVCRQEL------PSASGPSSSQN 245

The following BLAST results are available for this feature:

BLAST of MS004557 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7024775.1	2.5e-127	70.81	E3 ubiquitin-protein ligase RZF1, partial [Cucurbita argyrosperma subsp. argyros...	[more]
KAG6591901.1	2.5e-127	70.81	E3 ubiquitin-protein ligase RZF1, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_022975873.1	9.6e-127	71.12	E3 ubiquitin-protein ligase RZF1-like [Cucurbita maxima]	[more]
XP_022937137.1	2.1e-126	70.50	E3 ubiquitin-protein ligase RZF1-like [Cucurbita moschata] >XP_022937138.1 E3 ub...	[more]
XP_023535798.1	7.6e-124	69.57	E3 ubiquitin-protein ligase RZF1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS004557 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LPN7	1.1e-37	35.36	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
O22197	6.2e-36	36.17	Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
P0CH30	4.0e-35	35.69	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
Q94AK4	9.9e-34	36.21	E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV...	[more]
Q940T5	7.6e-26	30.23	E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ...	[more]

BLAST of MS004557 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IKH7	4.7e-127	71.12	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111476446 P...	[more]
A0A6J1FAA6	1.0e-126	70.50	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111443524...	[more]
A0A6J1FTR5	5.2e-118	67.48	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111446755...	[more]
A0A6J1ISV3	1.7e-116	70.10	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111480302 P...	[more]
A0A5A7TUM6	5.0e-113	63.33	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]

BLAST of MS004557 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G60360.1	6.1e-63	42.36	RING/U-box superfamily protein	[more]
AT3G19950.1	8.0e-39	35.36	RING/U-box superfamily protein	[more]
AT2G40830.2	4.4e-37	36.17	RING-H2 finger C1A	[more]
AT2G40830.1	4.4e-37	36.17	RING-H2 finger C1A	[more]
AT2G40830.3	4.4e-37	36.17	RING-H2 finger C1A	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 225..265 e-value: 3.7E-7 score: 39.8
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 224..266 e-value: 5.5E-11 score: 42.6
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 225..266 score: 12.061441
IPR039525	E3 ubiquitin-protein ligase RNF126-like, zinc-ribbon	PFAM	PF14369	zinc_ribbon_9	coord: 19..52 e-value: 2.8E-13 score: 49.9
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 188..272 e-value: 7.3E-22 score: 78.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 129..149
None	No IPR available	PANTHER	PTHR15710:SF29	SUBFAMILY NOT NAMED	coord: 149..283
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 149..283
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 11..97
None	No IPR available	PANTHER	PTHR15710:SF29	SUBFAMILY NOT NAMED	coord: 11..97
None	No IPR available	CDD	cd16667	RING-H2_RNF126_like	coord: 225..266 e-value: 3.88165E-22 score: 85.4472
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 205..273

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS004557.1	MS004557.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0061630	ubiquitin protein ligase activity