MS004095 (gene) Bitter gourd (TR) v1

Overview
NameMS004095
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionProtein DETOXIFICATION
Locationscaffold92: 35566 .. 39053 (-)
RNA-Seq ExpressionMS004095
SyntenyMS004095
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGACGGGAAGGAGCGCGCCATGGAAGAGATCTTACTGCCGAAGCAGAGGGATAACAAGGCCTCTTCAACGTCCATGGATTCTTTCTTTCATGAGGTGAAGAATGTGGGATTCTTGGCGGCGCCGATGGTTGCCGTCACTTTGTCGCAGTTTTTGTTGCAGATGATCACCATGATGATGGTCGGTCATCTTAGCGCACTGGCTCTTTCTAGCACCGCCATAGCCGTTTCCATATCTGGAGTTACAGGCTTCAGTGTTATCGTGAGTACTAATTTATGCTTTCATCTTTTTACATATTGTATGGTTGAGATTCTTTGTGTACCTTTTCCCCTCTGTTAGTACGAGCTACGTATAAATGTTGATGGATACTTTAATGGAGAAGGTTTAATCTCATGATTCTCGTCATCTAAAGAATACCCTTGCGTTTACGTCTCGTCTTTCTTGGAGAATAATTGCATTTTGTTTTGTTTATTCCCAAACTAGGTATAATTGTATTTTTTTTTCTTTTACTTTTCGTTGACTCAATGCATTGTTATTGAAGAAAATAGAATGGAGGAATTTGTCTTTTGTTGAATTTGAATCTCTCTTTTCAGCAACAGTATCAACCTAAGGGATAGACTGCAGGAGTCATCTCCTCCGTTAGATTAAGAGTTATCATTAAGTAAACAATTATGTTGAATAATTGTCATAACTCTCTCCAGCATAAATAAATGCATCTGTAGTTTATTTTGGATGTGATAATTGCTTTTTGAAAGCTAATTTTGACAAAATCGGACAGGAAATAAAGCCATCGAGCAAAATTAAGTTAGCAATTAGAGCTGCGTACTGGTGCTTCTGAACTGTAATTTCGTGAGCATAATGATGCCATTTTGTTCGTCTCTCTTTCCCTTGTCATTTAAGATTGGATTCAACCCATATTATGGTTAGAACAAGCCACTGTCATATTGCATCAATCTCAATGTTTCTTATGCCATAAACGTAGGGAACAACTATGAAATTCCGAGTACGTCTAAGACTACTAATCCACTGTAAAATGCTCTCTTCAAATTCCAACCTTTAGACCTATTGTTATAAACCCGACAAAGCTAGTCGATTTCAAATATATTTTGCTCTGCCAAGTCGCTAAACTAAATTCATTTGTTCTTGTTCCAACAACTGCCTGGATTTTTACATTGATACCAAACAGTTAGGCTTGTCCAGTGCCCTCGAAACTCTATGTGGACAAGCTTATGGAGCTCAGCAATATCACAAACTTGGAATTCAGACTTACACTGCTATATTTTGCATCATCTTAATTTGCATTCCTCTATCTCTATCTTGGATCTTCTTGGAAAAGCTACTCATTTTCCTTGGCCAAGATCCTCTGATTTCGCGCGAAGCTGGAAAATTCATTGTTTGGCTGATTCCTGCACTCTTTGCTTATGCATTTCTTCAACCACTGGTGAGATATTTCCAGGCACAAAGTTTAGTAATCCCCATGGTCGTTTGCTCTTGCATCACTCTCTGCTTCCACATACCATTTTGTTGGTTCATGGTGTACAAGTCTGGACTCAGTAATCTGGGAGGAGCATTGGCTATGAGTGTGTCAAATTGGTTGAATGTGATTTTGCTAGCATTATTCATGATATTATCGCCTAAATGTGAGAAAACTCGTGGAGTCATTTCTATGGAGCTATTTCGAGGAATTCGAGAGTTCTTTGGCTTGGCAATTCCTTCTGCGGTGATGATCTGGTGATTTATTATCCGTACTTTTTTTTATGTTTCGTAAATCATGAACCATGGATCTTTGTACTTTGATTTTCTTTTTTTTTTAATCTTTGAAAATAATAATTTAATGCTTATCATTGCAGCCTTGAATGGTGGTCATTTGAGCTGCTTATCTTACTATCTGGGCTGTTGCCGAATCCAGAGCTTGAAACTTCAGTTTTATCTGTTTGGTATATCTGCTCTATCTCATAGTGAATACAGAAACATCATTAACTTTCTTGTGTAAGCTAAAGCTTAGGGTAACTAGATGGAAAGCAGTTATATTAACAGTTGGCTCTTTTTAACAGTCTCAATACAATTGCAACTCTTTATTCGATACCATATGGAGTTGGTGCTGCAGGAAGGTATGCTCTAAGATACATGCATATGTAATTAATTTTTTGGATCCATAGATCTACACGCTTCAGCTATTAATGATCTTATCCATTAAATGGTGTTGTTTTTATTTGATGAAGAGGTCTTTGTACATTTATACTAGTTGCAGTGTTGACATTTGACTTCATATGATTGAGTAACAAGATAATTTGAAACTAATTTTAGTGACTAACCTGCATAATTAACTATTTACATATTATGAAGTGAACCTAAGCAATTGCTAAAATAACCCCAATAGAGAGAGAGTATAGGAATCGGATTTTGATTCCTTTGTTACAAATCATTTTCCTTTAAGGTAAAACAGTATCTTTTCTAGTCTCATGATAGTCATATGATTTATACTTGACATAAAATGGAACTGCAGTTTAGAGGTCCAATTCTTTAAATAAAAGGTTGAGGATTCAAGCTTTTATCAAAAGAAAAACAAAAAAAGAAATCAGGATTCAAAGTGATATAGGATATTCAATTGAGTATTTGTATTTTGCTTAAGCTATTAAATTATCATAAATTTAAGTTATTTAACCATTTAATATGTTCCCAACAAGTTGGAGAATTCATCATTTTGTAATACTTTTCCATTTGTTCCTGCTGCCATAGCACAAGAGTTTCCAACGAACTAGGTGCTGGGAACCCGCAAGCGGCTCGTCGAGCGACTGGTGCTGTTATATTCCTTGCCATTGTGGAAACCTCAATATTAAGCACAATGCTTTTTGCTCTTCGCCATGTCTTTGGTTATACCTTCAGCAATGAGAAGGATGTTGTGGCTTATGTGGCGTCTATGGCACCTCTGGTTTGCATATCCGTGATATTGGACAGCATACAGGGAGTCCTTTCAGGTTAAACATCTCTGCTACAAATCCGAAAAACTAAATCCTTCTGTGAAATTGTTATCTAATGAAATTACTACTAAAAATGATACATACATTCATGTTAGCTTTTAACTCTCCTTAGTCCTTTTGAGTGGATTTAAATTTTATTTTGGTTTACAATTCAAGAAATGACAATGTAGATACTTGAACCAGAAACTATTAAATTATGGTCAATATGTTCATCTATACTTATTTTCAGTACAAGTTGCTGCAGAGTTGAAATGTTTTTTTCCTTAACCAGGCATTGCTAGAGGATGTGGTTGGCAGCACATTGGTGCTTATGTAAACTTAGGGGCCTTCTATCTTTGTGGGATTCCAGTTGCTGCCATTCTTGCTTTCTGGGTGCAGCTGAGAGGAAGGGGTCTTTGGATTGGCATACAAATTGGGGCTTGTGTACAAACCATGTTGCTCGCCTTCATTACAAGTCGTATAAATTGGGAAGAACAGGTATCGTCTCCCACCTCAACTATCTAC

mRNA sequence

ATGGCGGACGGGAAGGAGCGCGCCATGGAAGAGATCTTACTGCCGAAGCAGAGGGATAACAAGGCCTCTTCAACGTCCATGGATTCTTTCTTTCATGAGGTGAAGAATGTGGGATTCTTGGCGGCGCCGATGGTTGCCGTCACTTTGTCGCAGTTTTTGTTGCAGATGATCACCATGATGATGGTCGGTCATCTTAGCGCACTGGCTCTTTCTAGCACCGCCATAGCCGTTTCCATATCTGGAGTTACAGGCTTCAGTGTTATCTTAGGCTTGTCCAGTGCCCTCGAAACTCTATGTGGACAAGCTTATGGAGCTCAGCAATATCACAAACTTGGAATTCAGACTTACACTGCTATATTTTGCATCATCTTAATTTGCATTCCTCTATCTCTATCTTGGATCTTCTTGGAAAAGCTACTCATTTTCCTTGGCCAAGATCCTCTGATTTCGCGCGAAGCTGGAAAATTCATTGTTTGGCTGATTCCTGCACTCTTTGCTTATGCATTTCTTCAACCACTGGTGAGATATTTCCAGGCACAAAGTTTAGTAATCCCCATGGTCGTTTGCTCTTGCATCACTCTCTGCTTCCACATACCATTTTGTTGGTTCATGGTGTACAAGTCTGGACTCAGTAATCTGGGAGGAGCATTGGCTATGAGTGTGTCAAATTGGTTGAATGTGATTTTGCTAGCATTATTCATGATATTATCGCCTAAATGTGAGAAAACTCGTGGAGTCATTTCTATGGAGCTATTTCGAGGAATTCGAGAGTTCTTTGGCTTGGCAATTCCTTCTGCGGTGATGATCTGCCTTGAATGGTGGTCATTTGAGCTGCTTATCTTACTATCTGGGCTGTTGCCGAATCCAGAGCTTGAAACTTCAGTTTTATCTGTTTGTCTCAATACAATTGCAACTCTTTATTCGATACCATATGGAGTTGGTGCTGCAGGAAGCACAAGAGTTTCCAACGAACTAGGTGCTGGGAACCCGCAAGCGGCTCGTCGAGCGACTGGTGCTGTTATATTCCTTGCCATTGTGGAAACCTCAATATTAAGCACAATGCTTTTTGCTCTTCGCCATGTCTTTGGTTATACCTTCAGCAATGAGAAGGATGTTGTGGCTTATGTGGCGTCTATGGCACCTCTGGTTTGCATATCCGTGATATTGGACAGCATACAGGGAGTCCTTTCAGGCATTGCTAGAGGATGTGGTTGGCAGCACATTGGTGCTTATGTAAACTTAGGGGCCTTCTATCTTTGTGGGATTCCAGTTGCTGCCATTCTTGCTTTCTGGGTGCAGCTGAGAGGAAGGGGTCTTTGGATTGGCATACAAATTGGGGCTTGTGTACAAACCATGTTGCTCGCCTTCATTACAAGTCGTATAAATTGGGAAGAACAGGTATCGTCTCCCACCTCAACTATCTAC

Coding sequence (CDS)

ATGGCGGACGGGAAGGAGCGCGCCATGGAAGAGATCTTACTGCCGAAGCAGAGGGATAACAAGGCCTCTTCAACGTCCATGGATTCTTTCTTTCATGAGGTGAAGAATGTGGGATTCTTGGCGGCGCCGATGGTTGCCGTCACTTTGTCGCAGTTTTTGTTGCAGATGATCACCATGATGATGGTCGGTCATCTTAGCGCACTGGCTCTTTCTAGCACCGCCATAGCCGTTTCCATATCTGGAGTTACAGGCTTCAGTGTTATCTTAGGCTTGTCCAGTGCCCTCGAAACTCTATGTGGACAAGCTTATGGAGCTCAGCAATATCACAAACTTGGAATTCAGACTTACACTGCTATATTTTGCATCATCTTAATTTGCATTCCTCTATCTCTATCTTGGATCTTCTTGGAAAAGCTACTCATTTTCCTTGGCCAAGATCCTCTGATTTCGCGCGAAGCTGGAAAATTCATTGTTTGGCTGATTCCTGCACTCTTTGCTTATGCATTTCTTCAACCACTGGTGAGATATTTCCAGGCACAAAGTTTAGTAATCCCCATGGTCGTTTGCTCTTGCATCACTCTCTGCTTCCACATACCATTTTGTTGGTTCATGGTGTACAAGTCTGGACTCAGTAATCTGGGAGGAGCATTGGCTATGAGTGTGTCAAATTGGTTGAATGTGATTTTGCTAGCATTATTCATGATATTATCGCCTAAATGTGAGAAAACTCGTGGAGTCATTTCTATGGAGCTATTTCGAGGAATTCGAGAGTTCTTTGGCTTGGCAATTCCTTCTGCGGTGATGATCTGCCTTGAATGGTGGTCATTTGAGCTGCTTATCTTACTATCTGGGCTGTTGCCGAATCCAGAGCTTGAAACTTCAGTTTTATCTGTTTGTCTCAATACAATTGCAACTCTTTATTCGATACCATATGGAGTTGGTGCTGCAGGAAGCACAAGAGTTTCCAACGAACTAGGTGCTGGGAACCCGCAAGCGGCTCGTCGAGCGACTGGTGCTGTTATATTCCTTGCCATTGTGGAAACCTCAATATTAAGCACAATGCTTTTTGCTCTTCGCCATGTCTTTGGTTATACCTTCAGCAATGAGAAGGATGTTGTGGCTTATGTGGCGTCTATGGCACCTCTGGTTTGCATATCCGTGATATTGGACAGCATACAGGGAGTCCTTTCAGGCATTGCTAGAGGATGTGGTTGGCAGCACATTGGTGCTTATGTAAACTTAGGGGCCTTCTATCTTTGTGGGATTCCAGTTGCTGCCATTCTTGCTTTCTGGGTGCAGCTGAGAGGAAGGGGTCTTTGGATTGGCATACAAATTGGGGCTTGTGTACAAACCATGTTGCTCGCCTTCATTACAAGTCGTATAAATTGGGAAGAACAGGTATCGTCTCCCACCTCAACTATCTAC

Protein sequence

MADGKERAMEEILLPKQRDNKASSTSMDSFFHEVKNVGFLAAPMVAVTLSQFLLQMITMMMVGHLSALALSSTAIAVSISGVTGFSVILGLSSALETLCGQAYGAQQYHKLGIQTYTAIFCIILICIPLSLSWIFLEKLLIFLGQDPLISREAGKFIVWLIPALFAYAFLQPLVRYFQAQSLVIPMVVCSCITLCFHIPFCWFMVYKSGLSNLGGALAMSVSNWLNVILLALFMILSPKCEKTRGVISMELFRGIREFFGLAIPSAVMICLEWWSFELLILLSGLLPNPELETSVLSVCLNTIATLYSIPYGVGAAGSTRVSNELGAGNPQAARRATGAVIFLAIVETSILSTMLFALRHVFGYTFSNEKDVVAYVASMAPLVCISVILDSIQGVLSGIARGCGWQHIGAYVNLGAFYLCGIPVAAILAFWVQLRGRGLWIGIQIGACVQTMLLAFITSRINWEEQVSSPTSTIY
Homology
BLAST of MS004095 vs. NCBI nr
Match: XP_022150680.1 (protein DETOXIFICATION 12-like [Momordica charantia])

HSP 1 Score: 888.3 bits (2294), Expect = 3.0e-254
Identity = 462/466 (99.14%), Postives = 465/466 (99.79%), Query Frame = 0

Query: 1   MADGKERAMEEILLPKQRDNKASSTSMDSFFHEVKNVGFLAAPMVAVTLSQFLLQMITMM 60
           MADGKERAMEEILLPKQRDNKASSTSMDSFFHEVKNVGFLAAPMVAVTLSQFLLQMITMM
Sbjct: 1   MADGKERAMEEILLPKQRDNKASSTSMDSFFHEVKNVGFLAAPMVAVTLSQFLLQMITMM 60

Query: 61  MVGHLSALALSSTAIAVSISGVTGFSVILGLSSALETLCGQAYGAQQYHKLGIQTYTAIF 120
           MVGHLSALALSSTA+AVSISGVTGFSVILGLSSALETLCGQAYGAQQYHKLGIQTYTAIF
Sbjct: 61  MVGHLSALALSSTAMAVSISGVTGFSVILGLSSALETLCGQAYGAQQYHKLGIQTYTAIF 120

Query: 121 CIILICIPLSLSWIFLEKLLIFLGQDPLISREAGKFIVWLIPALFAYAFLQPLVRYFQAQ 180
           CIILICIPLSLSWIFLEKLLIFLGQDPLISREAGKFIVWLIPALFAYAFLQPLVRYFQAQ
Sbjct: 121 CIILICIPLSLSWIFLEKLLIFLGQDPLISREAGKFIVWLIPALFAYAFLQPLVRYFQAQ 180

Query: 181 SLVIPMVVCSCITLCFHIPFCWFMVYKSGLSNLGGALAMSVSNWLNVILLALFMILSPKC 240
           SLVIPMVVCSCITLCFHIPFCWFMVYKSGLSNLGGALAMSVSNWLNVILLALFMILSPKC
Sbjct: 181 SLVIPMVVCSCITLCFHIPFCWFMVYKSGLSNLGGALAMSVSNWLNVILLALFMILSPKC 240

Query: 241 EKTRGVISMELFRGIREFFGLAIPSAVMICLEWWSFELLILLSGLLPNPELETSVLSVCL 300
           EKTRGVISMELFRGIREFFGLAIPSAVM+CLEWWSFELLILLSGLLPNPELETSVLSVCL
Sbjct: 241 EKTRGVISMELFRGIREFFGLAIPSAVMVCLEWWSFELLILLSGLLPNPELETSVLSVCL 300

Query: 301 NTIATLYSIPYGVGAAGSTRVSNELGAGNPQAARRATGAVIFLAIVETSILSTMLFALRH 360
           NTIATLYSIPYGVGAAGSTRVSNELGAGNPQAARRATGAVIFLAIVETSILST LFALRH
Sbjct: 301 NTIATLYSIPYGVGAAGSTRVSNELGAGNPQAARRATGAVIFLAIVETSILSTTLFALRH 360

Query: 361 VFGYTFSNEKDVVAYVASMAPLVCISVILDSIQGVLSGIARGCGWQHIGAYVNLGAFYLC 420
           VFGYTFSNEKDVVAYVASMAPLVCISV+LDSIQGVLSGIARGCGWQHIGAYVNLGAFYLC
Sbjct: 361 VFGYTFSNEKDVVAYVASMAPLVCISVMLDSIQGVLSGIARGCGWQHIGAYVNLGAFYLC 420

Query: 421 GIPVAAILAFWVQLRGRGLWIGIQIGACVQTMLLAFITSRINWEEQ 467
           GIPVAAILAFWVQLRGRGLWIGIQIGACVQTMLLAFITSRINWEEQ
Sbjct: 421 GIPVAAILAFWVQLRGRGLWIGIQIGACVQTMLLAFITSRINWEEQ 466

BLAST of MS004095 vs. NCBI nr
Match: XP_038892623.1 (protein DETOXIFICATION 12-like [Benincasa hispida])

HSP 1 Score: 760.0 bits (1961), Expect = 1.2e-215
Identity = 389/466 (83.48%), Postives = 424/466 (90.99%), Query Frame = 0

Query: 1   MADGKERAMEEILLPKQRDNKASSTSMDSFFHEVKNVGFLAAPMVAVTLSQFLLQMITMM 60
           M +GK+ AMEE LL KQ++N  SSTS   F  E+K VG+LAAP+V VT SQF+LQ+IT+M
Sbjct: 1   MENGKDCAMEETLLAKQKENNLSSTSRGVFLEEMKRVGYLAAPLVIVTFSQFMLQIITLM 60

Query: 61  MVGHLSALALSSTAIAVSISGVTGFSVILGLSSALETLCGQAYGAQQYHKLGIQTYTAIF 120
           MVGHL ALALSSTAIA SIS VTGFSV+LGLSSALETLCGQAYGAQQY K+G+QTYT +F
Sbjct: 61  MVGHLGALALSSTAIAFSISAVTGFSVLLGLSSALETLCGQAYGAQQYQKVGVQTYTGMF 120

Query: 121 CIILICIPLSLSWIFLEKLLIFLGQDPLISREAGKFIVWLIPALFAYAFLQPLVRYFQAQ 180
           CI LICIPLSLSW+FLEKLL+F GQDPLIS EAGKFIVWLIP LF  AFLQPLVRYFQ+Q
Sbjct: 121 CIFLICIPLSLSWLFLEKLLLFAGQDPLISHEAGKFIVWLIPGLFGSAFLQPLVRYFQSQ 180

Query: 181 SLVIPMVVCSCITLCFHIPFCWFMVYKSGLSNLGGALAMSVSNWLNVILLALFMILSPKC 240
           SLVIPMV+ SCITLCFHIPFCW MVYK+GL NLGGAL+MS+S WLNVILLAL+M  SP+C
Sbjct: 181 SLVIPMVIFSCITLCFHIPFCWLMVYKAGLRNLGGALSMSLSYWLNVILLALYMKFSPRC 240

Query: 241 EKTRGVISMELFRGIREFFGLAIPSAVMICLEWWSFELLILLSGLLPNPELETSVLSVCL 300
           EKTRGVISMELF+GIREFF LAIPSAVM+CLEWWSFEL+ILLSGLLPNPELETSVLSVCL
Sbjct: 241 EKTRGVISMELFQGIREFFSLAIPSAVMVCLEWWSFELIILLSGLLPNPELETSVLSVCL 300

Query: 301 NTIATLYSIPYGVGAAGSTRVSNELGAGNPQAARRATGAVIFLAIVETSILSTMLFALRH 360
           NTIATLYSI YG+GAAGSTRVSNELGAGNPQAARRAT  V+FLAI+ETSILST+LFALRH
Sbjct: 301 NTIATLYSIAYGLGAAGSTRVSNELGAGNPQAARRATSVVVFLAILETSILSTILFALRH 360

Query: 361 VFGYTFSNEKDVVAYVASMAPLVCISVILDSIQGVLSGIARGCGWQHIGAYVNLGAFYLC 420
           VFGYTFSNEKDVVAYVASMAPL+C+SVILD IQGVLSGIARGCGWQHIGAYVNLGAFYLC
Sbjct: 361 VFGYTFSNEKDVVAYVASMAPLICVSVILDGIQGVLSGIARGCGWQHIGAYVNLGAFYLC 420

Query: 421 GIPVAAILAFWVQLRGRGLWIGIQIGACVQTMLLAFITSRINWEEQ 467
           G+PVAA+L F V LRGRGLWIGIQIGA VQ MLL+FIT RINWEEQ
Sbjct: 421 GVPVAALLGFLVHLRGRGLWIGIQIGAFVQAMLLSFITIRINWEEQ 466

BLAST of MS004095 vs. NCBI nr
Match: XP_022968578.1 (protein DETOXIFICATION 12-like [Cucurbita maxima])

HSP 1 Score: 756.5 bits (1952), Expect = 1.4e-214
Identity = 394/466 (84.55%), Postives = 422/466 (90.56%), Query Frame = 0

Query: 1   MADGKERAMEEILLPKQRDNKASSTSMDSFFHEVKNVGFLAAPMVAVTLSQFLLQMITMM 60
           M +GK+ A+EEILLPKQ   K S+ S ++F  EVK VGFLAAPMVAVTLSQFLLQM+TMM
Sbjct: 1   MENGKQGAVEEILLPKQ---KESNISREAFLEEVKRVGFLAAPMVAVTLSQFLLQMVTMM 60

Query: 61  MVGHLSALALSSTAIAVSISGVTGFSVILGLSSALETLCGQAYGAQQYHKLGIQTYTAIF 120
           MVGHL  LALSS+A+A+SISGVTGFSV+LGLSSALETLCGQAYGA+QY KLG QTYTAIF
Sbjct: 61  MVGHLGELALSSSAVAISISGVTGFSVLLGLSSALETLCGQAYGARQYQKLGTQTYTAIF 120

Query: 121 CIILICIPLSLSWIFLEKLLIFLGQDPLISREAGKFIVWLIPALFAYAFLQPLVRYFQAQ 180
           CI L  IP+SLSWIFLEKLLIF GQDP IS EAGKFIVWLIPALFA AFLQPLVRYFQAQ
Sbjct: 121 CIFLFSIPISLSWIFLEKLLIFFGQDPRISHEAGKFIVWLIPALFACAFLQPLVRYFQAQ 180

Query: 181 SLVIPMVVCSCITLCFHIPFCWFMVYKSGLSNLGGALAMSVSNWLNVILLALFMILSPKC 240
           SLVIPMV+CSCITL FH+PFCWFMVYK+ L NLGGALAMS+S WLNVILLAL+MI SPKC
Sbjct: 181 SLVIPMVICSCITLSFHVPFCWFMVYKAELRNLGGALAMSLSYWLNVILLALYMIFSPKC 240

Query: 241 EKTRGVISMELFRGIREFFGLAIPSAVMICLEWWSFELLILLSGLLPNPELETSVLSVCL 300
             TRGVISME+FRGI+EFF LAIPSAVM+CLEWWSFELLILLSGLLPNPELETSVLSVCL
Sbjct: 241 ATTRGVISMEMFRGIQEFFSLAIPSAVMVCLEWWSFELLILLSGLLPNPELETSVLSVCL 300

Query: 301 NTIATLYSIPYGVGAAGSTRVSNELGAGNPQAARRATGAVIFLAIVETSILSTMLFALRH 360
           NTIATLYSIPYG+GAAGSTRVSNELGAGNPQAARRAT   IFL IVETSIL++ LFALR 
Sbjct: 301 NTIATLYSIPYGLGAAGSTRVSNELGAGNPQAARRATSVAIFLGIVETSILTSTLFALRR 360

Query: 361 VFGYTFSNEKDVVAYVASMAPLVCISVILDSIQGVLSGIARGCGWQHIGAYVNLGAFYLC 420
           VFGY FSNEKDVV YVASMAPLVC+SVI DSIQGVLSG+ARGCGWQHIGAYVNLGAFYLC
Sbjct: 361 VFGYAFSNEKDVVDYVASMAPLVCLSVIFDSIQGVLSGVARGCGWQHIGAYVNLGAFYLC 420

Query: 421 GIPVAAILAFWVQLRGRGLWIGIQIGACVQTMLLAFITSRINWEEQ 467
           G+PVAAILAF V LRGRGLWIGIQ GA VQT LL+FITSRINWEEQ
Sbjct: 421 GVPVAAILAFLVHLRGRGLWIGIQTGAFVQTALLSFITSRINWEEQ 463

BLAST of MS004095 vs. NCBI nr
Match: XP_023541093.1 (protein DETOXIFICATION 12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 755.4 bits (1949), Expect = 3.0e-214
Identity = 393/466 (84.33%), Postives = 422/466 (90.56%), Query Frame = 0

Query: 1   MADGKERAMEEILLPKQRDNKASSTSMDSFFHEVKNVGFLAAPMVAVTLSQFLLQMITMM 60
           M +GK+ A+EEILLPKQ   K S+ S ++F  EVK VGFLAAPMVAVTLSQFLLQM+TMM
Sbjct: 1   MENGKQGAVEEILLPKQ---KESNLSREAFLEEVKRVGFLAAPMVAVTLSQFLLQMVTMM 60

Query: 61  MVGHLSALALSSTAIAVSISGVTGFSVILGLSSALETLCGQAYGAQQYHKLGIQTYTAIF 120
           MVGHL  LALSS+A+A+SISGVTGFSV+LGLSSALETLCGQAYGA+QYHKLG QTYTAIF
Sbjct: 61  MVGHLGELALSSSAVAISISGVTGFSVLLGLSSALETLCGQAYGARQYHKLGTQTYTAIF 120

Query: 121 CIILICIPLSLSWIFLEKLLIFLGQDPLISREAGKFIVWLIPALFAYAFLQPLVRYFQAQ 180
           CI L  IP+SLSWIFLEKLLIF GQDP IS EAGKFIVWLIPALFA AFLQPLVRYFQAQ
Sbjct: 121 CIFLFSIPISLSWIFLEKLLIFFGQDPRISHEAGKFIVWLIPALFACAFLQPLVRYFQAQ 180

Query: 181 SLVIPMVVCSCITLCFHIPFCWFMVYKSGLSNLGGALAMSVSNWLNVILLALFMILSPKC 240
           SLVIPMV+CS ITL FH+PFCWFMVYK+ L NLGGALAMS+S WLNVILLAL+MI SPKC
Sbjct: 181 SLVIPMVICSSITLSFHVPFCWFMVYKAELRNLGGALAMSLSYWLNVILLALYMIFSPKC 240

Query: 241 EKTRGVISMELFRGIREFFGLAIPSAVMICLEWWSFELLILLSGLLPNPELETSVLSVCL 300
             TRGVISME+FRGI+EFF LAIPSAVM+CLEWWSFELLILLSGLLPNPELETSVLSVCL
Sbjct: 241 ATTRGVISMEMFRGIQEFFSLAIPSAVMVCLEWWSFELLILLSGLLPNPELETSVLSVCL 300

Query: 301 NTIATLYSIPYGVGAAGSTRVSNELGAGNPQAARRATGAVIFLAIVETSILSTMLFALRH 360
           NTIATLYSIPYG+GAAGSTRVSNELGAGNPQAARRAT   IFL +VETSIL++ LFALR 
Sbjct: 301 NTIATLYSIPYGLGAAGSTRVSNELGAGNPQAARRATSVAIFLGVVETSILTSTLFALRR 360

Query: 361 VFGYTFSNEKDVVAYVASMAPLVCISVILDSIQGVLSGIARGCGWQHIGAYVNLGAFYLC 420
           VFGY FSNEKDVV YVASMAPLVC+SVI DSIQGVLSG+ARGCGWQHIGAYVNLGAFYLC
Sbjct: 361 VFGYAFSNEKDVVDYVASMAPLVCLSVIFDSIQGVLSGVARGCGWQHIGAYVNLGAFYLC 420

Query: 421 GIPVAAILAFWVQLRGRGLWIGIQIGACVQTMLLAFITSRINWEEQ 467
           G+PVAAILAF V LRGRGLWIGIQ GA VQT LL+FITSRINWEEQ
Sbjct: 421 GVPVAAILAFLVHLRGRGLWIGIQTGAFVQTALLSFITSRINWEEQ 463

BLAST of MS004095 vs. NCBI nr
Match: KAG6573927.1 (Protein DETOXIFICATION 12, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 753.4 bits (1944), Expect = 1.2e-213
Identity = 393/466 (84.33%), Postives = 422/466 (90.56%), Query Frame = 0

Query: 1   MADGKERAMEEILLPKQRDNKASSTSMDSFFHEVKNVGFLAAPMVAVTLSQFLLQMITMM 60
           M +GK+ A+EEILLPKQ   K S+ S ++F  EVK VGFLAAPMVAVTLSQFLLQM+TMM
Sbjct: 1   MENGKQGAVEEILLPKQ---KESNLSREAFVEEVKRVGFLAAPMVAVTLSQFLLQMVTMM 60

Query: 61  MVGHLSALALSSTAIAVSISGVTGFSVILGLSSALETLCGQAYGAQQYHKLGIQTYTAIF 120
           MVGHL  LALSS+A+A+SISGVTGFSV+LGLSSALETLCGQAYGA+QY KLG QTYTAIF
Sbjct: 61  MVGHLGELALSSSAVAISISGVTGFSVLLGLSSALETLCGQAYGARQYQKLGTQTYTAIF 120

Query: 121 CIILICIPLSLSWIFLEKLLIFLGQDPLISREAGKFIVWLIPALFAYAFLQPLVRYFQAQ 180
           CI L  IP+SLSWIFLEKLLIF GQDP IS EAGKFIVWLIPALFA AFLQPLVRYFQAQ
Sbjct: 121 CIFLFSIPISLSWIFLEKLLIFFGQDPRISHEAGKFIVWLIPALFACAFLQPLVRYFQAQ 180

Query: 181 SLVIPMVVCSCITLCFHIPFCWFMVYKSGLSNLGGALAMSVSNWLNVILLALFMILSPKC 240
           SLVIPMV+CS ITL FH+PFCWFMVYK+ L NLGGALAMS+S WLNVILLAL+MI SPKC
Sbjct: 181 SLVIPMVICSSITLSFHVPFCWFMVYKAELRNLGGALAMSLSYWLNVILLALYMIFSPKC 240

Query: 241 EKTRGVISMELFRGIREFFGLAIPSAVMICLEWWSFELLILLSGLLPNPELETSVLSVCL 300
             TRGVISME+FRGI+EFF LAIPSAVM+CLEWWSFELLILLSGLLPNPELETSVLSVCL
Sbjct: 241 ATTRGVISMEMFRGIQEFFSLAIPSAVMVCLEWWSFELLILLSGLLPNPELETSVLSVCL 300

Query: 301 NTIATLYSIPYGVGAAGSTRVSNELGAGNPQAARRATGAVIFLAIVETSILSTMLFALRH 360
           NTIATLYSIPYG+GAAGSTRVSNELGAGNPQAARRAT   IFL +VETSIL++ LFALR 
Sbjct: 301 NTIATLYSIPYGLGAAGSTRVSNELGAGNPQAARRATSVAIFLGVVETSILTSTLFALRR 360

Query: 361 VFGYTFSNEKDVVAYVASMAPLVCISVILDSIQGVLSGIARGCGWQHIGAYVNLGAFYLC 420
           VFGY FSNEKDVV YVASMAPLVC+SVILDSIQGVLSG+ARGCGWQHIGAYVNLGAFYLC
Sbjct: 361 VFGYAFSNEKDVVDYVASMAPLVCLSVILDSIQGVLSGVARGCGWQHIGAYVNLGAFYLC 420

Query: 421 GIPVAAILAFWVQLRGRGLWIGIQIGACVQTMLLAFITSRINWEEQ 467
           G+PVAAILAF V LRGRGLWIGIQ GA VQT LL+FITSRINWEEQ
Sbjct: 421 GVPVAAILAFLVHLRGRGLWIGIQTGAFVQTALLSFITSRINWEEQ 463

BLAST of MS004095 vs. ExPASy Swiss-Prot
Match: Q8L731 (Protein DETOXIFICATION 12 OS=Arabidopsis thaliana OX=3702 GN=DTX12 PE=2 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 9.3e-158
Identity = 284/463 (61.34%), Postives = 356/463 (76.89%), Query Frame = 0

Query: 6   ERAMEEILLPKQR-DNKASSTSMD-SFFHEVKNVGFLAAPMVAVTLSQFLLQMITMMMVG 65
           E   + +LLP +R +N   S   D SF  E+K + F AAPM AV ++QF+LQ+++MMMVG
Sbjct: 5   ESTKDRLLLPVERVENVTWSDLRDGSFTVELKRLIFFAAPMAAVVIAQFMLQIVSMMMVG 64

Query: 66  HLSALALSSTAIAVSISGVTGFSVILGLSSALETLCGQAYGAQQYHKLGIQTYTAIFCII 125
           HL  L+L+S ++A S   VTGFS I+GLS AL+TL GQAYGA+ Y KLG+QTYTA+FC+ 
Sbjct: 65  HLGNLSLASASLASSFCNVTGFSFIIGLSCALDTLSGQAYGAKLYRKLGVQTYTAMFCLA 124

Query: 126 LICIPLSLSWIFLEKLLIFLGQDPLISREAGKFIVWLIPALFAYAFLQPLVRYFQAQSLV 185
           L+C+PLSL W  +EKLL+ LGQDP I+ EAGK+  WLIP LFAYA LQPL RYFQ QSL+
Sbjct: 125 LVCLPLSLIWFNMEKLLLILGQDPSIAHEAGKYATWLIPGLFAYAVLQPLTRYFQNQSLI 184

Query: 186 IPMVVCSCITLCFHIPFCWFMVYKSGLSNLGGALAMSVSNWLNVILLALFMILSPKCEKT 245
            P+++ S +  C H+P CWF+VY SGL NLGGALA+S+SNWL  I L  FM  S  C +T
Sbjct: 185 TPLLITSYVVFCIHVPLCWFLVYNSGLGNLGGALAISLSNWLYAIFLGSFMYYSSACSET 244

Query: 246 RGVISMELFRGIREFFGLAIPSAVMICLEWWSFELLILLSGLLPNPELETSVLSVCLNTI 305
           R  +SME+F GI EFF  A+PSA MICLEWWS+EL+ILLSGLLPNP+LETSVLSVCL TI
Sbjct: 245 RAPLSMEIFDGIGEFFKYALPSAAMICLEWWSYELIILLSGLLPNPQLETSVLSVCLQTI 304

Query: 306 ATLYSIPYGVGAAGSTRVSNELGAGNPQAARRATGAVIFLAIVETSILSTMLFALRHVFG 365
           +T+YSIP  + AA STR+SNELGAGN +AA     A + LA+++  I+S  L   R++FG
Sbjct: 305 STMYSIPLAIAAAASTRISNELGAGNSRAAHIVVYAAMSLAVIDALIVSMSLLIGRNLFG 364

Query: 366 YTFSNEKDVVAYVASMAPLVCISVILDSIQGVLSGIARGCGWQHIGAYVNLGAFYLCGIP 425
           + FS++K+ + YVA MAPLV IS++LD++QGVLSGIARGCGWQHIGAY+NLGAFYL GIP
Sbjct: 365 HIFSSDKETIDYVAKMAPLVSISLMLDALQGVLSGIARGCGWQHIGAYINLGAFYLWGIP 424

Query: 426 VAAILAFWVQLRGRGLWIGIQIGACVQTMLLAFITSRINWEEQ 467
           +AA LAFW+ L+G GLWIGIQ GA +QT+LLA +T   NWE Q
Sbjct: 425 IAASLAFWIHLKGVGLWIGIQAGAVLQTLLLALVTGCTNWESQ 467

BLAST of MS004095 vs. ExPASy Swiss-Prot
Match: Q94AL1 (Protein DETOXIFICATION 13 OS=Arabidopsis thaliana OX=3702 GN=DTX13 PE=2 SV=1)

HSP 1 Score: 542.0 bits (1395), Expect = 6.9e-153
Identity = 280/468 (59.83%), Postives = 350/468 (74.79%), Query Frame = 0

Query: 1   MADGKERAMEEILLPKQR-DNKASSTSMDSFF-HEVKNVGFLAAPMVAVTLSQFLLQMIT 60
           M D +  +   +LLP +R +N       D  F  E+K +   AAPM AV ++QF+LQ+I+
Sbjct: 1   MGDAESTSKTSLLLPVERVENVTWRDLRDGLFTAELKRLICFAAPMAAVVIAQFMLQIIS 60

Query: 61  MMMVGHLSALALSSTAIAVSISGVTGFSVILGLSSALETLCGQAYGAQQYHKLGIQTYTA 120
           M+MVGHL  L+L+S ++A S   VTGFS I+GLS AL+TL GQAYGA+ Y K+G+QTYTA
Sbjct: 61  MVMVGHLGNLSLASASLASSFCNVTGFSFIVGLSCALDTLSGQAYGAKLYRKVGVQTYTA 120

Query: 121 IFCIILICIPLSLSWIFLEKLLIFLGQDPLISREAGKFIVWLIPALFAYAFLQPLVRYFQ 180
           +FC+ L+C+PL+L W+ +E LL+FLGQDP I+ EAG++   LIP LFAYA LQPL RYFQ
Sbjct: 121 MFCLALVCLPLTLIWLNMETLLVFLGQDPSIAHEAGRYAACLIPGLFAYAVLQPLTRYFQ 180

Query: 181 AQSLVIPMVVCSCITLCFHIPFCWFMVYKSGLSNLGGALAMSVSNWLNVILLALFMILSP 240
            QS++ P+++ SC   C H+P CW +VYKSGL NLGGALA+S SN L  I+L   M  S 
Sbjct: 181 NQSMITPLLITSCFVFCLHVPLCWLLVYKSGLGNLGGALALSFSNCLYTIILGSLMCFSS 240

Query: 241 KCEKTRGVISMELFRGIREFFGLAIPSAVMICLEWWSFELLILLSGLLPNPELETSVLSV 300
            C +TR  +SME+F GI EFF  A+PSA MICLEWWS+EL+ILLSGLLPNP+LETSVLSV
Sbjct: 241 ACSETRAPLSMEIFDGIGEFFRYALPSAAMICLEWWSYELIILLSGLLPNPQLETSVLSV 300

Query: 301 CLNTIATLYSIPYGVGAAGSTRVSNELGAGNPQAARRATGAVIFLAIVETSILSTMLFAL 360
           CL T AT+YSI   + AA STR+SNELGAGN +AA     A + LA+VE  ILST L   
Sbjct: 301 CLQTTATVYSIHLAIAAAASTRISNELGAGNSRAANIVVYAAMSLAVVEILILSTSLLVG 360

Query: 361 RHVFGYTFSNEKDVVAYVASMAPLVCISVILDSIQGVLSGIARGCGWQHIGAYVNLGAFY 420
           R+VFG+ FS++K+ + YVA MAPLV IS+ILD +QGVLSGIARGCGWQHIGAY+NLGAFY
Sbjct: 361 RNVFGHVFSSDKETIDYVAKMAPLVSISLILDGLQGVLSGIARGCGWQHIGAYINLGAFY 420

Query: 421 LCGIPVAAILAFWVQLRGRGLWIGIQIGACVQTMLLAFITSRINWEEQ 467
           L GIP+AA LAFW+ L+G GLWIGIQ GA +QT+LL  +T   NWE Q
Sbjct: 421 LWGIPIAASLAFWIHLKGVGLWIGIQAGAVLQTLLLTLVTGCTNWESQ 468

BLAST of MS004095 vs. ExPASy Swiss-Prot
Match: F4HZH9 (Protein DETOXIFICATION 11 OS=Arabidopsis thaliana OX=3702 GN=DTX11 PE=3 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 1.2e-149
Identity = 263/438 (60.05%), Postives = 335/438 (76.48%), Query Frame = 0

Query: 29  SFFHEVKNVGFLAAPMVAVTLSQFLLQMITMMMVGHLSALALSSTAIAVSISGVTGFSVI 88
           SF  E+K +   AAPM AV ++Q +LQ+ITM++VGHL  L+L+S + A+S   VTGFS I
Sbjct: 27  SFTAELKKLICFAAPMAAVVITQSMLQIITMVIVGHLGRLSLASASFAISFCNVTGFSFI 86

Query: 89  LGLSSALETLCGQAYGAQQYHKLGIQTYTAIFCIILICIPLSLSWIFLEKLLIFLGQDPL 148
           +GLS AL+TL GQAYGA+ Y KLG+Q YTA+FC+ L+C+PLSL W  + KLL+ LGQDP 
Sbjct: 87  MGLSCALDTLSGQAYGAKLYRKLGVQAYTAMFCLTLVCLPLSLLWFNMGKLLVILGQDPS 146

Query: 149 ISREAGKFIVWLIPALFAYAFLQPLVRYFQAQSLVIPMVVCSCITLCFHIPFCWFMVYKS 208
           I+ EAG+F  WLIP LFAYA LQPL RYF+ QSL+ P+++ SC+  C H+P CW +VYKS
Sbjct: 147 IAHEAGRFAAWLIPGLFAYAVLQPLTRYFKNQSLITPLLITSCVVFCLHVPLCWLLVYKS 206

Query: 209 GLSNLGGALAMSVSNWLNVILLALFMILSPKCEKTRGVISMELFRGIREFFGLAIPSAVM 268
           GL ++GGALA+S+S WL  I L  FM  S  C +TR  ++ME+F G+REF   A+PSA M
Sbjct: 207 GLDHIGGALALSLSYWLYAIFLGSFMYFSSACSETRAPLTMEIFEGVREFIKYALPSAAM 266

Query: 269 ICLEWWSFELLILLSGLLPNPELETSVLSVCLNTIATLYSIPYGVGAAGSTRVSNELGAG 328
           +CLEWWS+EL+ILLSGLLPNP+LETSVLSVCL T++  YSIP  + AA STR+SNELGAG
Sbjct: 267 LCLEWWSYELIILLSGLLPNPQLETSVLSVCLQTLSMTYSIPLAIAAAASTRISNELGAG 326

Query: 329 NPQAARRATGAVIFLAIVETSILSTMLFALRHVFGYTFSNEKDVVAYVASMAPLVCISVI 388
           N +AA     A + LA+V+  ++ T L A +++ G  FS++K+ + YVA MAPLV IS+I
Sbjct: 327 NSRAAHIVVYAAMSLAVVDALMVGTSLLAGKNLLGQVFSSDKNTIDYVAKMAPLVSISLI 386

Query: 389 LDSIQGVLSGIARGCGWQHIGAYVNLGAFYLCGIPVAAILAFWVQLRGRGLWIGIQIGAC 448
           LDS+QGVLSG+A GCGWQHIGAY+N GAFYL GIP+AA LAFWV L+G GLWIGI  GA 
Sbjct: 387 LDSLQGVLSGVASGCGWQHIGAYINFGAFYLWGIPIAASLAFWVHLKGVGLWIGIIAGAV 446

Query: 449 VQTMLLAFITSRINWEEQ 467
           +QT+LLA +T  INWE Q
Sbjct: 447 LQTLLLALVTGCINWENQ 464

BLAST of MS004095 vs. ExPASy Swiss-Prot
Match: Q8VYL8 (Protein DETOXIFICATION 10 OS=Arabidopsis thaliana OX=3702 GN=DTX10 PE=2 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 2.7e-149
Identity = 261/438 (59.59%), Postives = 334/438 (76.26%), Query Frame = 0

Query: 29  SFFHEVKNVGFLAAPMVAVTLSQFLLQMITMMMVGHLSALALSSTAIAVSISGVTGFSVI 88
           SF  E+K +   AAPM AV + QF++Q+I+M+MVGHL  L+L+S + AVS   VTGFS I
Sbjct: 27  SFTAELKRLICFAAPMAAVVIIQFMIQIISMVMVGHLGRLSLASASFAVSFCNVTGFSFI 86

Query: 89  LGLSSALETLCGQAYGAQQYHKLGIQTYTAIFCIILICIPLSLSWIFLEKLLIFLGQDPL 148
           +GLS AL+TL GQAYGA+ Y KLG+Q YTA+FC+ L+C+PLSL W  + KL++ LGQDP 
Sbjct: 87  IGLSCALDTLSGQAYGAKLYRKLGVQAYTAMFCLTLVCLPLSLLWFNMGKLIVILGQDPA 146

Query: 149 ISREAGKFIVWLIPALFAYAFLQPLVRYFQAQSLVIPMVVCSCITLCFHIPFCWFMVYKS 208
           I+ EAG++  WLIP LFAYA LQPL+RYF+ QSL+ P++V S +  C H+P CW +VYKS
Sbjct: 147 IAHEAGRYAAWLIPGLFAYAVLQPLIRYFKNQSLITPLLVTSSVVFCIHVPLCWLLVYKS 206

Query: 209 GLSNLGGALAMSVSNWLNVILLALFMILSPKCEKTRGVISMELFRGIREFFGLAIPSAVM 268
           GL ++GGALA+S+S WL  I L  FM  S  C +TR  ++ME+F G+REF   A+PSA M
Sbjct: 207 GLGHIGGALALSLSYWLYAIFLGSFMYYSSACSETRAPLTMEIFEGVREFIKYALPSAAM 266

Query: 269 ICLEWWSFELLILLSGLLPNPELETSVLSVCLNTIATLYSIPYGVGAAGSTRVSNELGAG 328
           +CLEWWS+EL+ILLSGLLPNP+LETSVLS+C  T++  YSIP  + AA STR+SNELGAG
Sbjct: 267 LCLEWWSYELIILLSGLLPNPQLETSVLSICFETLSITYSIPLAIAAAASTRISNELGAG 326

Query: 329 NPQAARRATGAVIFLAIVETSILSTMLFALRHVFGYTFSNEKDVVAYVASMAPLVCISVI 388
           N +AA     A + LA+++  ++S  L A RHVFG+ FS++K  + YVA MAPLV IS+I
Sbjct: 327 NSRAAHIVVYAAMSLAVMDALMVSMSLLAGRHVFGHVFSSDKKTIEYVAKMAPLVSISII 386

Query: 389 LDSIQGVLSGIARGCGWQHIGAYVNLGAFYLCGIPVAAILAFWVQLRGRGLWIGIQIGAC 448
           LDS+QGVLSG+A GCGWQHIGAY+N GAFYL GIP+AA LAFWV L+G GLWIGI  GA 
Sbjct: 387 LDSLQGVLSGVASGCGWQHIGAYINFGAFYLWGIPIAASLAFWVHLKGVGLWIGILAGAV 446

Query: 449 VQTMLLAFITSRINWEEQ 467
           +QT+LLA +T   NW+ Q
Sbjct: 447 LQTLLLALVTGCTNWKTQ 464

BLAST of MS004095 vs. ExPASy Swiss-Prot
Match: Q9C994 (Protein DETOXIFICATION 14 OS=Arabidopsis thaliana OX=3702 GN=DTX14 PE=1 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 6.5e-143
Identity = 258/439 (58.77%), Postives = 329/439 (74.94%), Query Frame = 0

Query: 28  DSFFHEVKNVGFLAAPMVAVTLSQFLLQMITMMMVGHLSALALSSTAIAVSISGVTGFSV 87
           D F  E K + ++A PM+AV  S ++LQ+I++MMVGHL  L LSSTAIAVS   VTGFSV
Sbjct: 21  DGFLRETKKLSYIAGPMIAVNSSMYVLQVISIMMVGHLGELFLSSTAIAVSFCSVTGFSV 80

Query: 88  ILGLSSALETLCGQAYGAQQYHKLGIQTYTAIFCIILICIPLSLSWIFLEKLLIFLGQDP 147
           + GL+SALETLCGQA GA+QY KLG+ TYT I  + L+CIPLSL W ++  +L  +GQD 
Sbjct: 81  VFGLASALETLCGQANGAKQYEKLGVHTYTGIVSLFLVCIPLSLLWTYIGDILSLIGQDA 140

Query: 148 LISREAGKFIVWLIPALFAYAFLQPLVRYFQAQSLVIPMVVCSCITLCFHIPFCWFMVYK 207
           ++++EAGKF  WLIPALF YA LQPLVR+FQAQSL++P+V+ S  +LC HI  CW +V+K
Sbjct: 141 MVAQEAGKFATWLIPALFGYATLQPLVRFFQAQSLILPLVMSSVSSLCIHIVLCWSLVFK 200

Query: 208 SGLSNLGGALAMSVSNWLNVILLALFMILSPKCEKTRGVISMELFRGIREFFGLAIPSAV 267
            GL +LG A+A+ VS WLNV +L L+M  S  C K+R  ISM LF G+ EFF   IPSA 
Sbjct: 201 FGLGSLGAAIAIGVSYWLNVTVLGLYMTFSSSCSKSRATISMSLFEGMGEFFRFGIPSAS 260

Query: 268 MICLEWWSFELLILLSGLLPNPELETSVLSVCLNTIATLYSIPYGVGAAGSTRVSNELGA 327
           MICLEWWSFE L+LLSG+LPNP+LE SVLSVCL+T ++LY IP  +GAA STRV+NELGA
Sbjct: 261 MICLEWWSFEFLVLLSGILPNPKLEASVLSVCLSTQSSLYQIPESLGAAASTRVANELGA 320

Query: 328 GNPQAARRATGAVIFLAIVETSILSTMLFALRHVFGYTFSNEKDVVAYVASMAPLVCISV 387
           GNP+ AR A    + +  VE+ ++  ++F  R+VFGY FS+E +VV YV SMAPL+ +SV
Sbjct: 321 GNPKQARMAVYTAMVITGVESIMVGAIVFGARNVFGYLFSSETEVVDYVKSMAPLLSLSV 380

Query: 388 ILDSIQGVLSGIARGCGWQHIGAYVNLGAFYLCGIPVAAILAFWVQLRGRGLWIGIQIGA 447
           I D++   LSG+ARG G Q IGAYVNL A+YL GIP A +LAF  ++RGRGLWIGI +G+
Sbjct: 381 IFDALHAALSGVARGSGRQDIGAYVNLAAYYLFGIPTAILLAFGFKMRGRGLWIGITVGS 440

Query: 448 CVQTMLLAFITSRINWEEQ 467
           CVQ +LL  I    NW++Q
Sbjct: 441 CVQAVLLGLIVILTNWKKQ 459

BLAST of MS004095 vs. ExPASy TrEMBL
Match: A0A6J1D968 (Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111018752 PE=3 SV=1)

HSP 1 Score: 888.3 bits (2294), Expect = 1.5e-254
Identity = 462/466 (99.14%), Postives = 465/466 (99.79%), Query Frame = 0

Query: 1   MADGKERAMEEILLPKQRDNKASSTSMDSFFHEVKNVGFLAAPMVAVTLSQFLLQMITMM 60
           MADGKERAMEEILLPKQRDNKASSTSMDSFFHEVKNVGFLAAPMVAVTLSQFLLQMITMM
Sbjct: 1   MADGKERAMEEILLPKQRDNKASSTSMDSFFHEVKNVGFLAAPMVAVTLSQFLLQMITMM 60

Query: 61  MVGHLSALALSSTAIAVSISGVTGFSVILGLSSALETLCGQAYGAQQYHKLGIQTYTAIF 120
           MVGHLSALALSSTA+AVSISGVTGFSVILGLSSALETLCGQAYGAQQYHKLGIQTYTAIF
Sbjct: 61  MVGHLSALALSSTAMAVSISGVTGFSVILGLSSALETLCGQAYGAQQYHKLGIQTYTAIF 120

Query: 121 CIILICIPLSLSWIFLEKLLIFLGQDPLISREAGKFIVWLIPALFAYAFLQPLVRYFQAQ 180
           CIILICIPLSLSWIFLEKLLIFLGQDPLISREAGKFIVWLIPALFAYAFLQPLVRYFQAQ
Sbjct: 121 CIILICIPLSLSWIFLEKLLIFLGQDPLISREAGKFIVWLIPALFAYAFLQPLVRYFQAQ 180

Query: 181 SLVIPMVVCSCITLCFHIPFCWFMVYKSGLSNLGGALAMSVSNWLNVILLALFMILSPKC 240
           SLVIPMVVCSCITLCFHIPFCWFMVYKSGLSNLGGALAMSVSNWLNVILLALFMILSPKC
Sbjct: 181 SLVIPMVVCSCITLCFHIPFCWFMVYKSGLSNLGGALAMSVSNWLNVILLALFMILSPKC 240

Query: 241 EKTRGVISMELFRGIREFFGLAIPSAVMICLEWWSFELLILLSGLLPNPELETSVLSVCL 300
           EKTRGVISMELFRGIREFFGLAIPSAVM+CLEWWSFELLILLSGLLPNPELETSVLSVCL
Sbjct: 241 EKTRGVISMELFRGIREFFGLAIPSAVMVCLEWWSFELLILLSGLLPNPELETSVLSVCL 300

Query: 301 NTIATLYSIPYGVGAAGSTRVSNELGAGNPQAARRATGAVIFLAIVETSILSTMLFALRH 360
           NTIATLYSIPYGVGAAGSTRVSNELGAGNPQAARRATGAVIFLAIVETSILST LFALRH
Sbjct: 301 NTIATLYSIPYGVGAAGSTRVSNELGAGNPQAARRATGAVIFLAIVETSILSTTLFALRH 360

Query: 361 VFGYTFSNEKDVVAYVASMAPLVCISVILDSIQGVLSGIARGCGWQHIGAYVNLGAFYLC 420
           VFGYTFSNEKDVVAYVASMAPLVCISV+LDSIQGVLSGIARGCGWQHIGAYVNLGAFYLC
Sbjct: 361 VFGYTFSNEKDVVAYVASMAPLVCISVMLDSIQGVLSGIARGCGWQHIGAYVNLGAFYLC 420

Query: 421 GIPVAAILAFWVQLRGRGLWIGIQIGACVQTMLLAFITSRINWEEQ 467
           GIPVAAILAFWVQLRGRGLWIGIQIGACVQTMLLAFITSRINWEEQ
Sbjct: 421 GIPVAAILAFWVQLRGRGLWIGIQIGACVQTMLLAFITSRINWEEQ 466

BLAST of MS004095 vs. ExPASy TrEMBL
Match: A0A6J1HYF5 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111467760 PE=3 SV=1)

HSP 1 Score: 756.5 bits (1952), Expect = 6.6e-215
Identity = 394/466 (84.55%), Postives = 422/466 (90.56%), Query Frame = 0

Query: 1   MADGKERAMEEILLPKQRDNKASSTSMDSFFHEVKNVGFLAAPMVAVTLSQFLLQMITMM 60
           M +GK+ A+EEILLPKQ   K S+ S ++F  EVK VGFLAAPMVAVTLSQFLLQM+TMM
Sbjct: 1   MENGKQGAVEEILLPKQ---KESNISREAFLEEVKRVGFLAAPMVAVTLSQFLLQMVTMM 60

Query: 61  MVGHLSALALSSTAIAVSISGVTGFSVILGLSSALETLCGQAYGAQQYHKLGIQTYTAIF 120
           MVGHL  LALSS+A+A+SISGVTGFSV+LGLSSALETLCGQAYGA+QY KLG QTYTAIF
Sbjct: 61  MVGHLGELALSSSAVAISISGVTGFSVLLGLSSALETLCGQAYGARQYQKLGTQTYTAIF 120

Query: 121 CIILICIPLSLSWIFLEKLLIFLGQDPLISREAGKFIVWLIPALFAYAFLQPLVRYFQAQ 180
           CI L  IP+SLSWIFLEKLLIF GQDP IS EAGKFIVWLIPALFA AFLQPLVRYFQAQ
Sbjct: 121 CIFLFSIPISLSWIFLEKLLIFFGQDPRISHEAGKFIVWLIPALFACAFLQPLVRYFQAQ 180

Query: 181 SLVIPMVVCSCITLCFHIPFCWFMVYKSGLSNLGGALAMSVSNWLNVILLALFMILSPKC 240
           SLVIPMV+CSCITL FH+PFCWFMVYK+ L NLGGALAMS+S WLNVILLAL+MI SPKC
Sbjct: 181 SLVIPMVICSCITLSFHVPFCWFMVYKAELRNLGGALAMSLSYWLNVILLALYMIFSPKC 240

Query: 241 EKTRGVISMELFRGIREFFGLAIPSAVMICLEWWSFELLILLSGLLPNPELETSVLSVCL 300
             TRGVISME+FRGI+EFF LAIPSAVM+CLEWWSFELLILLSGLLPNPELETSVLSVCL
Sbjct: 241 ATTRGVISMEMFRGIQEFFSLAIPSAVMVCLEWWSFELLILLSGLLPNPELETSVLSVCL 300

Query: 301 NTIATLYSIPYGVGAAGSTRVSNELGAGNPQAARRATGAVIFLAIVETSILSTMLFALRH 360
           NTIATLYSIPYG+GAAGSTRVSNELGAGNPQAARRAT   IFL IVETSIL++ LFALR 
Sbjct: 301 NTIATLYSIPYGLGAAGSTRVSNELGAGNPQAARRATSVAIFLGIVETSILTSTLFALRR 360

Query: 361 VFGYTFSNEKDVVAYVASMAPLVCISVILDSIQGVLSGIARGCGWQHIGAYVNLGAFYLC 420
           VFGY FSNEKDVV YVASMAPLVC+SVI DSIQGVLSG+ARGCGWQHIGAYVNLGAFYLC
Sbjct: 361 VFGYAFSNEKDVVDYVASMAPLVCLSVIFDSIQGVLSGVARGCGWQHIGAYVNLGAFYLC 420

Query: 421 GIPVAAILAFWVQLRGRGLWIGIQIGACVQTMLLAFITSRINWEEQ 467
           G+PVAAILAF V LRGRGLWIGIQ GA VQT LL+FITSRINWEEQ
Sbjct: 421 GVPVAAILAFLVHLRGRGLWIGIQTGAFVQTALLSFITSRINWEEQ 463

BLAST of MS004095 vs. ExPASy TrEMBL
Match: A0A6J1G211 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111449944 PE=3 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 1.6e-213
Identity = 392/466 (84.12%), Postives = 421/466 (90.34%), Query Frame = 0

Query: 1   MADGKERAMEEILLPKQRDNKASSTSMDSFFHEVKNVGFLAAPMVAVTLSQFLLQMITMM 60
           M +GK+ A+EEILLPKQ   K S+ S ++F  EVK VGFLAAPMVAVTLSQFLLQM+TMM
Sbjct: 1   MENGKQGAVEEILLPKQ---KESNLSREAFVEEVKRVGFLAAPMVAVTLSQFLLQMVTMM 60

Query: 61  MVGHLSALALSSTAIAVSISGVTGFSVILGLSSALETLCGQAYGAQQYHKLGIQTYTAIF 120
           MVGHL  LALSS+A+A+SISGVTGFSV+LGLSSALETLCGQAYGA+QY KLG QTYTAIF
Sbjct: 61  MVGHLGELALSSSAVAISISGVTGFSVLLGLSSALETLCGQAYGARQYQKLGTQTYTAIF 120

Query: 121 CIILICIPLSLSWIFLEKLLIFLGQDPLISREAGKFIVWLIPALFAYAFLQPLVRYFQAQ 180
           CI L  IP+SLSWIFLEKLLIF GQDP IS EAGKFIVWLIPALFA AFLQPLVRYFQAQ
Sbjct: 121 CIFLFSIPISLSWIFLEKLLIFFGQDPRISHEAGKFIVWLIPALFACAFLQPLVRYFQAQ 180

Query: 181 SLVIPMVVCSCITLCFHIPFCWFMVYKSGLSNLGGALAMSVSNWLNVILLALFMILSPKC 240
           SLVIPMV+CS ITL FH+PFCWFMVYK+ L NLGGALAMS+S WLNVILLAL+MI SPKC
Sbjct: 181 SLVIPMVICSSITLSFHVPFCWFMVYKAELRNLGGALAMSLSYWLNVILLALYMIFSPKC 240

Query: 241 EKTRGVISMELFRGIREFFGLAIPSAVMICLEWWSFELLILLSGLLPNPELETSVLSVCL 300
             TRGVISME+FRGI+EFF LAIPSAVM+CLEWWSFELLILLSGLLPNPELETSVLSVCL
Sbjct: 241 ATTRGVISMEMFRGIQEFFSLAIPSAVMVCLEWWSFELLILLSGLLPNPELETSVLSVCL 300

Query: 301 NTIATLYSIPYGVGAAGSTRVSNELGAGNPQAARRATGAVIFLAIVETSILSTMLFALRH 360
           NTIATLYSIPYG+GAAGSTRVSNELGAGNPQAARRAT   IFL +VETSIL++ LFALR 
Sbjct: 301 NTIATLYSIPYGLGAAGSTRVSNELGAGNPQAARRATSVAIFLGVVETSILTSTLFALRR 360

Query: 361 VFGYTFSNEKDVVAYVASMAPLVCISVILDSIQGVLSGIARGCGWQHIGAYVNLGAFYLC 420
           VFGY FSNEKDVV YVASMAPLVC+SVI DSIQGVLSG+ARGCGWQHIGAYVNLGAFYLC
Sbjct: 361 VFGYAFSNEKDVVDYVASMAPLVCLSVIFDSIQGVLSGVARGCGWQHIGAYVNLGAFYLC 420

Query: 421 GIPVAAILAFWVQLRGRGLWIGIQIGACVQTMLLAFITSRINWEEQ 467
           G+PVAAILAF V LRGRGLWIGIQ GA VQT LL+FITSRINWEEQ
Sbjct: 421 GVPVAAILAFLVHLRGRGLWIGIQTGAFVQTALLSFITSRINWEEQ 463

BLAST of MS004095 vs. ExPASy TrEMBL
Match: A0A1S3BGB1 (Protein DETOXIFICATION OS=Cucumis melo OX=3656 GN=LOC103489328 PE=3 SV=1)

HSP 1 Score: 741.9 bits (1914), Expect = 1.7e-210
Identity = 381/466 (81.76%), Postives = 418/466 (89.70%), Query Frame = 0

Query: 1   MADGKERAMEEILLPKQRDNKASSTSMDSFFHEVKNVGFLAAPMVAVTLSQFLLQMITMM 60
           M +  + +MEE LL KQ++N  SSTS   +  E++ VGFLAAP+V VT SQF+LQ+ITMM
Sbjct: 1   MENRNDCSMEETLLAKQKENNLSSTSTGVYLEEMRRVGFLAAPLVIVTFSQFMLQIITMM 60

Query: 61  MVGHLSALALSSTAIAVSISGVTGFSVILGLSSALETLCGQAYGAQQYHKLGIQTYTAIF 120
           MVGHL ALALSSTAIAVSIS VTGFSV+LGLSSALETLCGQAYGAQQY K+GIQTYTAIF
Sbjct: 61  MVGHLGALALSSTAIAVSISAVTGFSVLLGLSSALETLCGQAYGAQQYQKVGIQTYTAIF 120

Query: 121 CIILICIPLSLSWIFLEKLLIFLGQDPLISREAGKFIVWLIPALFAYAFLQPLVRYFQAQ 180
           CI LIC PLSL W+FLEKLL+F+GQDPLIS EAGKFIVWLIP LFA AFLQPLVRYFQAQ
Sbjct: 121 CIFLICFPLSLVWLFLEKLLLFVGQDPLISHEAGKFIVWLIPGLFACAFLQPLVRYFQAQ 180

Query: 181 SLVIPMVVCSCITLCFHIPFCWFMVYKSGLSNLGGALAMSVSNWLNVILLALFMILSPKC 240
           SLVIPMV+ SCITLCFHIP CWFMVYK+GL NLGGAL+MS S WLNVILLAL+M  SPKC
Sbjct: 181 SLVIPMVIFSCITLCFHIPICWFMVYKTGLRNLGGALSMSFSYWLNVILLALYMKFSPKC 240

Query: 241 EKTRGVISMELFRGIREFFGLAIPSAVMICLEWWSFELLILLSGLLPNPELETSVLSVCL 300
           EKTRGVISMELF+GIR+FF LA+PSAVM+CLEWWSFEL+ILLSGLLPNPELETSVLSVCL
Sbjct: 241 EKTRGVISMELFQGIRDFFSLAVPSAVMVCLEWWSFELIILLSGLLPNPELETSVLSVCL 300

Query: 301 NTIATLYSIPYGVGAAGSTRVSNELGAGNPQAARRATGAVIFLAIVETSILSTMLFALRH 360
            TIA+LYSI YG+GAAGSTRVSNELGAGNPQAARRAT  V+FLAI+ET ILST LFALRH
Sbjct: 301 QTIASLYSIAYGLGAAGSTRVSNELGAGNPQAARRATRVVLFLAILETLILSTTLFALRH 360

Query: 361 VFGYTFSNEKDVVAYVASMAPLVCISVILDSIQGVLSGIARGCGWQHIGAYVNLGAFYLC 420
           +FGYTFSNEKDVV YVASMAPL+CISV+LD IQGVLSGIARGCGWQH+GAYVN G+FYLC
Sbjct: 361 IFGYTFSNEKDVVDYVASMAPLICISVLLDGIQGVLSGIARGCGWQHLGAYVNFGSFYLC 420

Query: 421 GIPVAAILAFWVQLRGRGLWIGIQIGACVQTMLLAFITSRINWEEQ 467
           GIPVAA+L F V L+GRGLWIGIQIGA VQ  LL+FITSRINWEEQ
Sbjct: 421 GIPVAALLGFLVHLKGRGLWIGIQIGAFVQASLLSFITSRINWEEQ 466

BLAST of MS004095 vs. ExPASy TrEMBL
Match: A0A0A0KRA2 (Protein DETOXIFICATION OS=Cucumis sativus OX=3659 GN=Csa_5G611100 PE=3 SV=1)

HSP 1 Score: 728.0 bits (1878), Expect = 2.5e-206
Identity = 375/466 (80.47%), Postives = 416/466 (89.27%), Query Frame = 0

Query: 1   MADGKERAMEEILLPKQRDNKASSTSMDSFFHEVKNVGFLAAPMVAVTLSQFLLQMITMM 60
           M + K  +MEE LL KQ++   SST+   +  E+K VGFLAAP+V VT SQF+LQ+ITMM
Sbjct: 1   MENRKNCSMEESLLAKQKETNLSSTTTAVYLEEMKRVGFLAAPLVIVTFSQFMLQIITMM 60

Query: 61  MVGHLSALALSSTAIAVSISGVTGFSVILGLSSALETLCGQAYGAQQYHKLGIQTYTAIF 120
           MVGHL ALALSSTAIAVSIS VTGFSV+LGLSSALETLCGQAYGAQQ+ K+G+QTYTAIF
Sbjct: 61  MVGHLGALALSSTAIAVSISAVTGFSVLLGLSSALETLCGQAYGAQQFEKVGVQTYTAIF 120

Query: 121 CIILICIPLSLSWIFLEKLLIFLGQDPLISREAGKFIVWLIPALFAYAFLQPLVRYFQAQ 180
           C+ LIC PLSL W+FLEKLL+F+GQDPLIS EAGKFIVWLIP LFA AFLQPLVRYFQAQ
Sbjct: 121 CVFLICFPLSLIWLFLEKLLLFVGQDPLISHEAGKFIVWLIPGLFASAFLQPLVRYFQAQ 180

Query: 181 SLVIPMVVCSCITLCFHIPFCWFMVYKSGLSNLGGALAMSVSNWLNVILLALFMILSPKC 240
           SLVIPMV+ SCITL FHIP CWFMVYK+GL NLGGAL+MS S  LNVILLAL+M  SPKC
Sbjct: 181 SLVIPMVIFSCITLFFHIPLCWFMVYKTGLRNLGGALSMSFSYCLNVILLALYMKFSPKC 240

Query: 241 EKTRGVISMELFRGIREFFGLAIPSAVMICLEWWSFELLILLSGLLPNPELETSVLSVCL 300
           EKTRGVISMELF+GIR+FF LA+PSAVM+CLEWWSFEL+ILLSGLLPNPELETSVLSVCL
Sbjct: 241 EKTRGVISMELFQGIRDFFSLAVPSAVMVCLEWWSFELIILLSGLLPNPELETSVLSVCL 300

Query: 301 NTIATLYSIPYGVGAAGSTRVSNELGAGNPQAARRATGAVIFLAIVETSILSTMLFALRH 360
            TIA+LYSI YG+GAAGSTRVSNELGAGNPQAARRAT  V+FLAI+ET  LST+LFALRH
Sbjct: 301 QTIASLYSIAYGLGAAGSTRVSNELGAGNPQAARRATRVVLFLAILETLTLSTILFALRH 360

Query: 361 VFGYTFSNEKDVVAYVASMAPLVCISVILDSIQGVLSGIARGCGWQHIGAYVNLGAFYLC 420
           +FGYTFSNEKDVVAYVASMAPLVCISV++D IQGVLSGIARGCGWQHIGAYVN G+FYLC
Sbjct: 361 IFGYTFSNEKDVVAYVASMAPLVCISVLMDGIQGVLSGIARGCGWQHIGAYVNFGSFYLC 420

Query: 421 GIPVAAILAFWVQLRGRGLWIGIQIGACVQTMLLAFITSRINWEEQ 467
           GIPVAA+L F V ++GRGLWIGIQ+GA VQ  LL+FITSRINWEEQ
Sbjct: 421 GIPVAALLGFLVHMKGRGLWIGIQMGAFVQVTLLSFITSRINWEEQ 466

BLAST of MS004095 vs. TAIR 10
Match: AT1G15170.1 (MATE efflux family protein )

HSP 1 Score: 558.1 bits (1437), Expect = 6.6e-159
Identity = 284/463 (61.34%), Postives = 356/463 (76.89%), Query Frame = 0

Query: 6   ERAMEEILLPKQR-DNKASSTSMD-SFFHEVKNVGFLAAPMVAVTLSQFLLQMITMMMVG 65
           E   + +LLP +R +N   S   D SF  E+K + F AAPM AV ++QF+LQ+++MMMVG
Sbjct: 5   ESTKDRLLLPVERVENVTWSDLRDGSFTVELKRLIFFAAPMAAVVIAQFMLQIVSMMMVG 64

Query: 66  HLSALALSSTAIAVSISGVTGFSVILGLSSALETLCGQAYGAQQYHKLGIQTYTAIFCII 125
           HL  L+L+S ++A S   VTGFS I+GLS AL+TL GQAYGA+ Y KLG+QTYTA+FC+ 
Sbjct: 65  HLGNLSLASASLASSFCNVTGFSFIIGLSCALDTLSGQAYGAKLYRKLGVQTYTAMFCLA 124

Query: 126 LICIPLSLSWIFLEKLLIFLGQDPLISREAGKFIVWLIPALFAYAFLQPLVRYFQAQSLV 185
           L+C+PLSL W  +EKLL+ LGQDP I+ EAGK+  WLIP LFAYA LQPL RYFQ QSL+
Sbjct: 125 LVCLPLSLIWFNMEKLLLILGQDPSIAHEAGKYATWLIPGLFAYAVLQPLTRYFQNQSLI 184

Query: 186 IPMVVCSCITLCFHIPFCWFMVYKSGLSNLGGALAMSVSNWLNVILLALFMILSPKCEKT 245
            P+++ S +  C H+P CWF+VY SGL NLGGALA+S+SNWL  I L  FM  S  C +T
Sbjct: 185 TPLLITSYVVFCIHVPLCWFLVYNSGLGNLGGALAISLSNWLYAIFLGSFMYYSSACSET 244

Query: 246 RGVISMELFRGIREFFGLAIPSAVMICLEWWSFELLILLSGLLPNPELETSVLSVCLNTI 305
           R  +SME+F GI EFF  A+PSA MICLEWWS+EL+ILLSGLLPNP+LETSVLSVCL TI
Sbjct: 245 RAPLSMEIFDGIGEFFKYALPSAAMICLEWWSYELIILLSGLLPNPQLETSVLSVCLQTI 304

Query: 306 ATLYSIPYGVGAAGSTRVSNELGAGNPQAARRATGAVIFLAIVETSILSTMLFALRHVFG 365
           +T+YSIP  + AA STR+SNELGAGN +AA     A + LA+++  I+S  L   R++FG
Sbjct: 305 STMYSIPLAIAAAASTRISNELGAGNSRAAHIVVYAAMSLAVIDALIVSMSLLIGRNLFG 364

Query: 366 YTFSNEKDVVAYVASMAPLVCISVILDSIQGVLSGIARGCGWQHIGAYVNLGAFYLCGIP 425
           + FS++K+ + YVA MAPLV IS++LD++QGVLSGIARGCGWQHIGAY+NLGAFYL GIP
Sbjct: 365 HIFSSDKETIDYVAKMAPLVSISLMLDALQGVLSGIARGCGWQHIGAYINLGAFYLWGIP 424

Query: 426 VAAILAFWVQLRGRGLWIGIQIGACVQTMLLAFITSRINWEEQ 467
           +AA LAFW+ L+G GLWIGIQ GA +QT+LLA +T   NWE Q
Sbjct: 425 IAASLAFWIHLKGVGLWIGIQAGAVLQTLLLALVTGCTNWESQ 467

BLAST of MS004095 vs. TAIR 10
Match: AT1G15180.1 (MATE efflux family protein )

HSP 1 Score: 542.0 bits (1395), Expect = 4.9e-154
Identity = 280/468 (59.83%), Postives = 350/468 (74.79%), Query Frame = 0

Query: 1   MADGKERAMEEILLPKQR-DNKASSTSMDSFF-HEVKNVGFLAAPMVAVTLSQFLLQMIT 60
           M D +  +   +LLP +R +N       D  F  E+K +   AAPM AV ++QF+LQ+I+
Sbjct: 1   MGDAESTSKTSLLLPVERVENVTWRDLRDGLFTAELKRLICFAAPMAAVVIAQFMLQIIS 60

Query: 61  MMMVGHLSALALSSTAIAVSISGVTGFSVILGLSSALETLCGQAYGAQQYHKLGIQTYTA 120
           M+MVGHL  L+L+S ++A S   VTGFS I+GLS AL+TL GQAYGA+ Y K+G+QTYTA
Sbjct: 61  MVMVGHLGNLSLASASLASSFCNVTGFSFIVGLSCALDTLSGQAYGAKLYRKVGVQTYTA 120

Query: 121 IFCIILICIPLSLSWIFLEKLLIFLGQDPLISREAGKFIVWLIPALFAYAFLQPLVRYFQ 180
           +FC+ L+C+PL+L W+ +E LL+FLGQDP I+ EAG++   LIP LFAYA LQPL RYFQ
Sbjct: 121 MFCLALVCLPLTLIWLNMETLLVFLGQDPSIAHEAGRYAACLIPGLFAYAVLQPLTRYFQ 180

Query: 181 AQSLVIPMVVCSCITLCFHIPFCWFMVYKSGLSNLGGALAMSVSNWLNVILLALFMILSP 240
            QS++ P+++ SC   C H+P CW +VYKSGL NLGGALA+S SN L  I+L   M  S 
Sbjct: 181 NQSMITPLLITSCFVFCLHVPLCWLLVYKSGLGNLGGALALSFSNCLYTIILGSLMCFSS 240

Query: 241 KCEKTRGVISMELFRGIREFFGLAIPSAVMICLEWWSFELLILLSGLLPNPELETSVLSV 300
            C +TR  +SME+F GI EFF  A+PSA MICLEWWS+EL+ILLSGLLPNP+LETSVLSV
Sbjct: 241 ACSETRAPLSMEIFDGIGEFFRYALPSAAMICLEWWSYELIILLSGLLPNPQLETSVLSV 300

Query: 301 CLNTIATLYSIPYGVGAAGSTRVSNELGAGNPQAARRATGAVIFLAIVETSILSTMLFAL 360
           CL T AT+YSI   + AA STR+SNELGAGN +AA     A + LA+VE  ILST L   
Sbjct: 301 CLQTTATVYSIHLAIAAAASTRISNELGAGNSRAANIVVYAAMSLAVVEILILSTSLLVG 360

Query: 361 RHVFGYTFSNEKDVVAYVASMAPLVCISVILDSIQGVLSGIARGCGWQHIGAYVNLGAFY 420
           R+VFG+ FS++K+ + YVA MAPLV IS+ILD +QGVLSGIARGCGWQHIGAY+NLGAFY
Sbjct: 361 RNVFGHVFSSDKETIDYVAKMAPLVSISLILDGLQGVLSGIARGCGWQHIGAYINLGAFY 420

Query: 421 LCGIPVAAILAFWVQLRGRGLWIGIQIGACVQTMLLAFITSRINWEEQ 467
           L GIP+AA LAFW+ L+G GLWIGIQ GA +QT+LL  +T   NWE Q
Sbjct: 421 LWGIPIAASLAFWIHLKGVGLWIGIQAGAVLQTLLLTLVTGCTNWESQ 468

BLAST of MS004095 vs. TAIR 10
Match: AT1G15160.1 (MATE efflux family protein )

HSP 1 Score: 531.2 bits (1367), Expect = 8.7e-151
Identity = 263/438 (60.05%), Postives = 335/438 (76.48%), Query Frame = 0

Query: 29  SFFHEVKNVGFLAAPMVAVTLSQFLLQMITMMMVGHLSALALSSTAIAVSISGVTGFSVI 88
           SF  E+K +   AAPM AV ++Q +LQ+ITM++VGHL  L+L+S + A+S   VTGFS I
Sbjct: 27  SFTAELKKLICFAAPMAAVVITQSMLQIITMVIVGHLGRLSLASASFAISFCNVTGFSFI 86

Query: 89  LGLSSALETLCGQAYGAQQYHKLGIQTYTAIFCIILICIPLSLSWIFLEKLLIFLGQDPL 148
           +GLS AL+TL GQAYGA+ Y KLG+Q YTA+FC+ L+C+PLSL W  + KLL+ LGQDP 
Sbjct: 87  MGLSCALDTLSGQAYGAKLYRKLGVQAYTAMFCLTLVCLPLSLLWFNMGKLLVILGQDPS 146

Query: 149 ISREAGKFIVWLIPALFAYAFLQPLVRYFQAQSLVIPMVVCSCITLCFHIPFCWFMVYKS 208
           I+ EAG+F  WLIP LFAYA LQPL RYF+ QSL+ P+++ SC+  C H+P CW +VYKS
Sbjct: 147 IAHEAGRFAAWLIPGLFAYAVLQPLTRYFKNQSLITPLLITSCVVFCLHVPLCWLLVYKS 206

Query: 209 GLSNLGGALAMSVSNWLNVILLALFMILSPKCEKTRGVISMELFRGIREFFGLAIPSAVM 268
           GL ++GGALA+S+S WL  I L  FM  S  C +TR  ++ME+F G+REF   A+PSA M
Sbjct: 207 GLDHIGGALALSLSYWLYAIFLGSFMYFSSACSETRAPLTMEIFEGVREFIKYALPSAAM 266

Query: 269 ICLEWWSFELLILLSGLLPNPELETSVLSVCLNTIATLYSIPYGVGAAGSTRVSNELGAG 328
           +CLEWWS+EL+ILLSGLLPNP+LETSVLSVCL T++  YSIP  + AA STR+SNELGAG
Sbjct: 267 LCLEWWSYELIILLSGLLPNPQLETSVLSVCLQTLSMTYSIPLAIAAAASTRISNELGAG 326

Query: 329 NPQAARRATGAVIFLAIVETSILSTMLFALRHVFGYTFSNEKDVVAYVASMAPLVCISVI 388
           N +AA     A + LA+V+  ++ T L A +++ G  FS++K+ + YVA MAPLV IS+I
Sbjct: 327 NSRAAHIVVYAAMSLAVVDALMVGTSLLAGKNLLGQVFSSDKNTIDYVAKMAPLVSISLI 386

Query: 389 LDSIQGVLSGIARGCGWQHIGAYVNLGAFYLCGIPVAAILAFWVQLRGRGLWIGIQIGAC 448
           LDS+QGVLSG+A GCGWQHIGAY+N GAFYL GIP+AA LAFWV L+G GLWIGI  GA 
Sbjct: 387 LDSLQGVLSGVASGCGWQHIGAYINFGAFYLWGIPIAASLAFWVHLKGVGLWIGIIAGAV 446

Query: 449 VQTMLLAFITSRINWEEQ 467
           +QT+LLA +T  INWE Q
Sbjct: 447 LQTLLLALVTGCINWENQ 464

BLAST of MS004095 vs. TAIR 10
Match: AT1G15150.1 (MATE efflux family protein )

HSP 1 Score: 530.0 bits (1364), Expect = 1.9e-150
Identity = 261/438 (59.59%), Postives = 334/438 (76.26%), Query Frame = 0

Query: 29  SFFHEVKNVGFLAAPMVAVTLSQFLLQMITMMMVGHLSALALSSTAIAVSISGVTGFSVI 88
           SF  E+K +   AAPM AV + QF++Q+I+M+MVGHL  L+L+S + AVS   VTGFS I
Sbjct: 27  SFTAELKRLICFAAPMAAVVIIQFMIQIISMVMVGHLGRLSLASASFAVSFCNVTGFSFI 86

Query: 89  LGLSSALETLCGQAYGAQQYHKLGIQTYTAIFCIILICIPLSLSWIFLEKLLIFLGQDPL 148
           +GLS AL+TL GQAYGA+ Y KLG+Q YTA+FC+ L+C+PLSL W  + KL++ LGQDP 
Sbjct: 87  IGLSCALDTLSGQAYGAKLYRKLGVQAYTAMFCLTLVCLPLSLLWFNMGKLIVILGQDPA 146

Query: 149 ISREAGKFIVWLIPALFAYAFLQPLVRYFQAQSLVIPMVVCSCITLCFHIPFCWFMVYKS 208
           I+ EAG++  WLIP LFAYA LQPL+RYF+ QSL+ P++V S +  C H+P CW +VYKS
Sbjct: 147 IAHEAGRYAAWLIPGLFAYAVLQPLIRYFKNQSLITPLLVTSSVVFCIHVPLCWLLVYKS 206

Query: 209 GLSNLGGALAMSVSNWLNVILLALFMILSPKCEKTRGVISMELFRGIREFFGLAIPSAVM 268
           GL ++GGALA+S+S WL  I L  FM  S  C +TR  ++ME+F G+REF   A+PSA M
Sbjct: 207 GLGHIGGALALSLSYWLYAIFLGSFMYYSSACSETRAPLTMEIFEGVREFIKYALPSAAM 266

Query: 269 ICLEWWSFELLILLSGLLPNPELETSVLSVCLNTIATLYSIPYGVGAAGSTRVSNELGAG 328
           +CLEWWS+EL+ILLSGLLPNP+LETSVLS+C  T++  YSIP  + AA STR+SNELGAG
Sbjct: 267 LCLEWWSYELIILLSGLLPNPQLETSVLSICFETLSITYSIPLAIAAAASTRISNELGAG 326

Query: 329 NPQAARRATGAVIFLAIVETSILSTMLFALRHVFGYTFSNEKDVVAYVASMAPLVCISVI 388
           N +AA     A + LA+++  ++S  L A RHVFG+ FS++K  + YVA MAPLV IS+I
Sbjct: 327 NSRAAHIVVYAAMSLAVMDALMVSMSLLAGRHVFGHVFSSDKKTIEYVAKMAPLVSISII 386

Query: 389 LDSIQGVLSGIARGCGWQHIGAYVNLGAFYLCGIPVAAILAFWVQLRGRGLWIGIQIGAC 448
           LDS+QGVLSG+A GCGWQHIGAY+N GAFYL GIP+AA LAFWV L+G GLWIGI  GA 
Sbjct: 387 LDSLQGVLSGVASGCGWQHIGAYINFGAFYLWGIPIAASLAFWVHLKGVGLWIGILAGAV 446

Query: 449 VQTMLLAFITSRINWEEQ 467
           +QT+LLA +T   NW+ Q
Sbjct: 447 LQTLLLALVTGCTNWKTQ 464

BLAST of MS004095 vs. TAIR 10
Match: AT1G71140.1 (MATE efflux family protein )

HSP 1 Score: 508.8 bits (1309), Expect = 4.6e-144
Identity = 258/439 (58.77%), Postives = 329/439 (74.94%), Query Frame = 0

Query: 28  DSFFHEVKNVGFLAAPMVAVTLSQFLLQMITMMMVGHLSALALSSTAIAVSISGVTGFSV 87
           D F  E K + ++A PM+AV  S ++LQ+I++MMVGHL  L LSSTAIAVS   VTGFSV
Sbjct: 21  DGFLRETKKLSYIAGPMIAVNSSMYVLQVISIMMVGHLGELFLSSTAIAVSFCSVTGFSV 80

Query: 88  ILGLSSALETLCGQAYGAQQYHKLGIQTYTAIFCIILICIPLSLSWIFLEKLLIFLGQDP 147
           + GL+SALETLCGQA GA+QY KLG+ TYT I  + L+CIPLSL W ++  +L  +GQD 
Sbjct: 81  VFGLASALETLCGQANGAKQYEKLGVHTYTGIVSLFLVCIPLSLLWTYIGDILSLIGQDA 140

Query: 148 LISREAGKFIVWLIPALFAYAFLQPLVRYFQAQSLVIPMVVCSCITLCFHIPFCWFMVYK 207
           ++++EAGKF  WLIPALF YA LQPLVR+FQAQSL++P+V+ S  +LC HI  CW +V+K
Sbjct: 141 MVAQEAGKFATWLIPALFGYATLQPLVRFFQAQSLILPLVMSSVSSLCIHIVLCWSLVFK 200

Query: 208 SGLSNLGGALAMSVSNWLNVILLALFMILSPKCEKTRGVISMELFRGIREFFGLAIPSAV 267
            GL +LG A+A+ VS WLNV +L L+M  S  C K+R  ISM LF G+ EFF   IPSA 
Sbjct: 201 FGLGSLGAAIAIGVSYWLNVTVLGLYMTFSSSCSKSRATISMSLFEGMGEFFRFGIPSAS 260

Query: 268 MICLEWWSFELLILLSGLLPNPELETSVLSVCLNTIATLYSIPYGVGAAGSTRVSNELGA 327
           MICLEWWSFE L+LLSG+LPNP+LE SVLSVCL+T ++LY IP  +GAA STRV+NELGA
Sbjct: 261 MICLEWWSFEFLVLLSGILPNPKLEASVLSVCLSTQSSLYQIPESLGAAASTRVANELGA 320

Query: 328 GNPQAARRATGAVIFLAIVETSILSTMLFALRHVFGYTFSNEKDVVAYVASMAPLVCISV 387
           GNP+ AR A    + +  VE+ ++  ++F  R+VFGY FS+E +VV YV SMAPL+ +SV
Sbjct: 321 GNPKQARMAVYTAMVITGVESIMVGAIVFGARNVFGYLFSSETEVVDYVKSMAPLLSLSV 380

Query: 388 ILDSIQGVLSGIARGCGWQHIGAYVNLGAFYLCGIPVAAILAFWVQLRGRGLWIGIQIGA 447
           I D++   LSG+ARG G Q IGAYVNL A+YL GIP A +LAF  ++RGRGLWIGI +G+
Sbjct: 381 IFDALHAALSGVARGSGRQDIGAYVNLAAYYLFGIPTAILLAFGFKMRGRGLWIGITVGS 440

Query: 448 CVQTMLLAFITSRINWEEQ 467
           CVQ +LL  I    NW++Q
Sbjct: 441 CVQAVLLGLIVILTNWKKQ 459

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022150680.13.0e-25499.14protein DETOXIFICATION 12-like [Momordica charantia][more]
XP_038892623.11.2e-21583.48protein DETOXIFICATION 12-like [Benincasa hispida][more]
XP_022968578.11.4e-21484.55protein DETOXIFICATION 12-like [Cucurbita maxima][more]
XP_023541093.13.0e-21484.33protein DETOXIFICATION 12-like [Cucurbita pepo subsp. pepo][more]
KAG6573927.11.2e-21384.33Protein DETOXIFICATION 12, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q8L7319.3e-15861.34Protein DETOXIFICATION 12 OS=Arabidopsis thaliana OX=3702 GN=DTX12 PE=2 SV=1[more]
Q94AL16.9e-15359.83Protein DETOXIFICATION 13 OS=Arabidopsis thaliana OX=3702 GN=DTX13 PE=2 SV=1[more]
F4HZH91.2e-14960.05Protein DETOXIFICATION 11 OS=Arabidopsis thaliana OX=3702 GN=DTX11 PE=3 SV=1[more]
Q8VYL82.7e-14959.59Protein DETOXIFICATION 10 OS=Arabidopsis thaliana OX=3702 GN=DTX10 PE=2 SV=1[more]
Q9C9946.5e-14358.77Protein DETOXIFICATION 14 OS=Arabidopsis thaliana OX=3702 GN=DTX14 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1D9681.5e-25499.14Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111018752 PE=3 SV=1[more]
A0A6J1HYF56.6e-21584.55Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111467760 PE=3 SV=1[more]
A0A6J1G2111.6e-21384.12Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111449944 PE=3 SV=1[more]
A0A1S3BGB11.7e-21081.76Protein DETOXIFICATION OS=Cucumis melo OX=3656 GN=LOC103489328 PE=3 SV=1[more]
A0A0A0KRA22.5e-20680.47Protein DETOXIFICATION OS=Cucumis sativus OX=3659 GN=Csa_5G611100 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G15170.16.6e-15961.34MATE efflux family protein [more]
AT1G15180.14.9e-15459.83MATE efflux family protein [more]
AT1G15160.18.7e-15160.05MATE efflux family protein [more]
AT1G15150.11.9e-15059.59MATE efflux family protein [more]
AT1G71140.14.6e-14458.77MATE efflux family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002528Multi antimicrobial extrusion proteinPFAMPF01554MatEcoord: 264..427
e-value: 3.1E-29
score: 101.7
coord: 43..202
e-value: 7.3E-32
score: 110.2
IPR002528Multi antimicrobial extrusion proteinTIGRFAMTIGR00797TIGR00797coord: 43..440
e-value: 1.7E-77
score: 258.9
NoneNo IPR availablePANTHERPTHR11206MULTIDRUG RESISTANCE PROTEINcoord: 30..468
NoneNo IPR availablePANTHERPTHR11206:SF388PROTEIN DETOXIFICATIONcoord: 30..468
IPR045069Multidrug and toxic compound extrusion family, eukaryoticCDDcd13132MATE_eukaryoticcoord: 33..468
e-value: 3.83228E-172
score: 489.006

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS004095.1MS004095.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
biological_process GO:1990961 xenobiotic detoxification by transmembrane export across the plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015297 antiporter activity
molecular_function GO:0042910 xenobiotic transmembrane transporter activity