MS003724 (gene) Bitter gourd (TR) v1

Overview


Name	MS003724
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Auxin-responsive protein
Location	scaffold127: 9136 .. 10200 (+)
RNA-Seq Expression	MS003724
Synteny	MS003724

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CAATGCGGTGAAAAAAAGGAACAGCAGGGCAACTGGGTCGGCCAAAGCCAGAGCATTGATACGGACATCTTAGACGTTTTTCGCCTCTGCCTCCAACACTTTGTCCCACCGCTCTCCGATTCTTGTCCCACACCCGCACGCACATCCCCCTTTACTATTAAACCTAAACCCCCTCCATACCATCAAACCCACATCTCTTCAATCCCATCTCCATACCCTTCTTTGTTCTTTTGCTGTTCCCATTCCCATTTTACCTTACCTTCATGGCTATGGAGCTTCAGCTCGGCCTTGCTCTAATTCCCACCAAAGGCTTTGATTTAAACACCCACGTCCACGACACTGCGCCTTCTTTCGAGAACTTGGACCCCGTGTGCGCCAAAAAGCGCGCTCTCGATTTCGACCCCTGCTCGGCATTTCGTCGAACACTTCCTCTGCTTCTCTGGAACCACCATCCAAATGACGGCGATGACGACCCGAAAGACATCCATCGCTCCTCCGCCTTCCGGCCCAATCAACGGTACCTAATTCTCTTTCTCTCTGAGGTTTCAATTTGGTCGATGCCGGGTCTGAATTTTTTGATTGGTTTGACAGAGATGCGGAGGAGGGGAATGGGCTGGTGGGGTGGCCGCCGCTTAAGAAACGGAGGAAGAGGCTTTTCATGGAGAAACGCGGACGTGGACGTGGACGTGGACGTGGACCGGCGGCGGAGAACCGCCGGCCGGTGGAGAATGGCGGCGGGTTTTACCGGGGGTCGAATTCTTCCACTTCCAATTCGCGGTACGTGAAGGTGAAGATGGAGGGAGTGGGAATTGCGAGGAAGGTTGACTTAAGCCTCCACCATTCTTTCGCGGCGCTCACAGCCACCCTAATGAACATGTTTGGTGAGTTTTAAAACTGCACCAGGCACGGGCTAAGATTACGTTCTTATCCAGAGTAGGAATAGTGAAAATATGAGCTTATACTGGTTTTGCGTATACAGATGAGACGGATTCCGCCGACGCTTACAGACTGACTTACCAGGACATTGACGGTGACTGGTTACTTGCTCAGGACGTAACTTGGAGG

mRNA sequence

CAATGCGGTGAAAAAAAGGAACAGCAGGGCAACTGGGTCGGCCAAAGCCAGAGCATTGATACGGACATCTTAGACGTTTTTCGCCTCTGCCTCCAACACTTTGTCCCACCGCTCTCCGATTCTTGTCCCACACCCGCACGCACATCCCCCTTTACTATTAAACCTAAACCCCCTCCATACCATCAAACCCACATCTCTTCACCCATCTCCATACCCTTCTTTGTTCTTTTGCTGTTCCCATTCCCATTTTACCTTACCTTCATGGCTATGGAGCTTCAGCTCGGCCTTGCTCTAATTCCCACCAAAGGCTTTGATTTAAACACCCACGTCCACGACACTGCGCCTTCTTTCGAGAACTTGGACCCCGTGTGCGCCAAAAAGCGCGCTCTCGATTTCGACCCCTGCTCGGCATTTCGTCGAACACTTCCTCTGCTTCTCTGGAACCACCATCCAAATGACGGCGATGACGACCCGAAAGACATCCATCGCTCCTCCGCCTTCCGGCCCAATCAACGAGATGCGGAGGAGGGGAATGGGCTGGTGGGGTGGCCGCCGCTTAAGAAACGGAGGAAGAGGCTTTTCATGGAGAAACGCGGACGTGGACGTGGACGTGGACGTGGACCGGCGGCGGAGAACCGCCGGCCGGTGGAGAATGGCGGCGGGTTTTACCGGGGGTCGAATTCTTCCACTTCCAATTCGCGGTACGTGAAGGTGAAGATGGAGGGAGTGGGAATTGCGAGGAAGGTTGACTTAAGCCTCCACCATTCTTTCGCGGCGCTCACAGCCACCCTAATGAACATGTTTGATGAGACGGATTCCGCCGACGCTTACAGACTGACTTACCAGGACATTGACGGTGACTGGTTACTTGCTCAGGACGTAACTTGGAGG

Coding sequence (CDS)

CAATGCGGTGAAAAAAAGGAACAGCAGGGCAACTGGGTCGGCCAAAGCCAGAGCATTGATACGGACATCTTAGACGTTTTTCGCCTCTGCCTCCAACACTTTGTCCCACCGCTCTCCGATTCTTGTCCCACACCCGCACGCACATCCCCCTTTACTATTAAACCTAAACCCCCTCCATACCATCAAACCCACATCTCTTCACCCATCTCCATACCCTTCTTTGTTCTTTTGCTGTTCCCATTCCCATTTTACCTTACCTTCATGGCTATGGAGCTTCAGCTCGGCCTTGCTCTAATTCCCACCAAAGGCTTTGATTTAAACACCCACGTCCACGACACTGCGCCTTCTTTCGAGAACTTGGACCCCGTGTGCGCCAAAAAGCGCGCTCTCGATTTCGACCCCTGCTCGGCATTTCGTCGAACACTTCCTCTGCTTCTCTGGAACCACCATCCAAATGACGGCGATGACGACCCGAAAGACATCCATCGCTCCTCCGCCTTCCGGCCCAATCAACGAGATGCGGAGGAGGGGAATGGGCTGGTGGGGTGGCCGCCGCTTAAGAAACGGAGGAAGAGGCTTTTCATGGAGAAACGCGGACGTGGACGTGGACGTGGACGTGGACCGGCGGCGGAGAACCGCCGGCCGGTGGAGAATGGCGGCGGGTTTTACCGGGGGTCGAATTCTTCCACTTCCAATTCGCGGTACGTGAAGGTGAAGATGGAGGGAGTGGGAATTGCGAGGAAGGTTGACTTAAGCCTCCACCATTCTTTCGCGGCGCTCACAGCCACCCTAATGAACATGTTTGATGAGACGGATTCCGCCGACGCTTACAGACTGACTTACCAGGACATTGACGGTGACTGGTTACTTGCTCAGGACGTAACTTGGAGG

Protein sequence

QCGEKKEQQGNWVGQSQSIDTDILDVFRLCLQHFVPPLSDSCPTPARTSPFTIKPKPPPYHQTHISSPISIPFFVLLLFPFPFYLTFMAMELQLGLALIPTKGFDLNTHVHDTAPSFENLDPVCAKKRALDFDPCSAFRRTLPLLLWNHHPNDGDDDPKDIHRSSAFRPNQRDAEEGNGLVGWPPLKKRRKRLFMEKRGRGRGRGRGPAAENRRPVENGGGFYRGSNSSTSNSRYVKVKMEGVGIARKVDLSLHHSFAALTATLMNMFDETDSADAYRLTYQDIDGDWLLAQDVTWR

Homology

[Close]

BLAST of MS003724 vs. NCBI nr
Match: XP_022144854.1 (auxin-responsive protein IAA29-like [Momordica charantia])

HSP 1 Score: 422.5 bits (1085), Expect = 2.9e-114
Identity = 207/212 (97.64%), Postives = 209/212 (98.58%), Query Frame = 0


Query: 88  MAMELQLGLALIPTKGFDLNTHVHDTAPSFENLDPVCAKKRALDFDPCSAFRRTLPLLLW 147
           MAMELQLGLALIPTKGFDLNTHVH+TAPSFE LDPVCAKKRALDFDPCSAFRRTLPLLLW
Sbjct: 1   MAMELQLGLALIPTKGFDLNTHVHETAPSFEYLDPVCAKKRALDFDPCSAFRRTLPLLLW 60

Query: 148 NHHPNDGDDDPKDIHRSSAFRPNQRDAEEGNGLVGWPPLKKRRKRLFMEKR--GRGRGRG 207
           NHHPNDGDDDPKDIHRSSAFRPN+RDAEEGNGLVGWPPLKKRRKRLFMEKR  GRGRGRG
Sbjct: 61  NHHPNDGDDDPKDIHRSSAFRPNRRDAEEGNGLVGWPPLKKRRKRLFMEKRGHGRGRGRG 120

Query: 208 RGPAAENRRPVENGGGFYRGSNSSTSNSRYVKVKMEGVGIARKVDLSLHHSFAALTATLM 267
           RGPAAENRRPVENGGGFYRGSNSSTSNSRYVKVKMEGVGIARKVDLSLHHSFAALTATLM
Sbjct: 121 RGPAAENRRPVENGGGFYRGSNSSTSNSRYVKVKMEGVGIARKVDLSLHHSFAALTATLM 180

Query: 268 NMFDETDSADAYRLTYQDIDGDWLLAQDVTWR 298
           NMFDETDSADAYRLTYQDIDGDWLLAQDVTWR
Sbjct: 181 NMFDETDSADAYRLTYQDIDGDWLLAQDVTWR 212

[Close]

BLAST of MS003724 vs. NCBI nr
Match: XP_022987761.1 (uncharacterized protein LOC111485214 isoform X2 [Cucurbita maxima])

HSP 1 Score: 282.3 bits (721), Expect = 4.7e-72
Identity = 176/301 (58.47%), Postives = 195/301 (64.78%), Query Frame = 0


Query: 6   KEQQGNWVGQSQSIDTDILDVFRLCLQHFVP-----PLSDSCPTPARTSPFTIKPKPPPY 65
           KEQQG+WVGQ QSIDTDI D FRL +QHFVP     PL   C T AR S    KP PPP+
Sbjct: 6   KEQQGSWVGQRQSIDTDISDHFRLSIQHFVPIHILVPLPHVC-THARPSSAPFKP-PPPF 65

Query: 66  HQTHISSPISIPFFVLLLFPFPFYLTFMAMELQLGLALI---PTKGFDLNTHVHDTAPSF 125
                SSP +         P    +  MAMELQLGLAL    PTK F LNTH H   P  
Sbjct: 66  FP---SSPTN---------PHVSSIPSMAMELQLGLALFPTNPTKPFHLNTH-HRALPCL 125

Query: 126 ENLDPVCAKKRALDFDPCSAFRRTLPLLLWNHHPNDGDDDPKDIHRSSAFRPNQRDAEEG 185
           +  D +  KKR LD DP SAFRRTLPLLLWN HPNDGDDDP D + +S+  P+    +E 
Sbjct: 126 D-FDSISGKKRPLDIDPSSAFRRTLPLLLWNDHPNDGDDDPNDPNSASSNHPDNPKEQEI 185

Query: 186 NGLVGWPPLKKRRKRLFMEKRGRGRGRGRGPAAENRRP-VENGGGFYRGSNSSTSNSRYV 245
             LVGWPPLKKRRK LF++KR R        A   RRP +ENGGG +R  NS  +NS YV
Sbjct: 186 E-LVGWPPLKKRRKTLFVQKRIR-------RAVVIRRPAMENGGGVHRELNS--NNSLYV 245

Query: 246 KVKMEGVGIARKVDLSLHHSFAALTATLMNMFDETDSADAYRLTYQDIDGDWLLAQDVTW 298
           KVKMEGVGI RKVD+  HH F AL ATLMNMFD+ DS D Y LTYQD DGDWLLA DVTW
Sbjct: 246 KVKMEGVGIGRKVDIRQHHCFDALRATLMNMFDKPDS-DGYNLTYQDTDGDWLLADDVTW 279

[Close]

BLAST of MS003724 vs. NCBI nr
Match: KGN65132.1 (hypothetical protein Csa_023489 [Cucumis sativus])

HSP 1 Score: 278.1 bits (710), Expect = 8.9e-71
Identity = 166/298 (55.70%), Postives = 200/298 (67.11%), Query Frame = 0


Query: 5   KKEQQGNWVGQSQSIDTDILDVFRLCLQHFVPPLSDSCPTPARTSPFTIKPKPPPYHQTH 64
           +KE + NWVGQ QSIDTDILDVF    QHFVP +   CPTPART+P  +    P  +  +
Sbjct: 5   EKEHESNWVGQRQSIDTDILDVFPFSFQHFVPTILILCPTPARTTPLLLLNPHPLSNFHN 64

Query: 65  ISSPISIPFFVLLLFPFPFYLTFMAMELQLGLALIPT---KGFDLNTHVHDTAPSFENLD 124
            +S   + F  + LFP+      MAMELQLGLALIPT   K FDLNT  H+  P     D
Sbjct: 65  SNSSFVLTFSSINLFPY-----LMAMELQLGLALIPTNPIKPFDLNT--HNNNPISLVFD 124

Query: 125 PVCAKKRALDFDPCSAFRRTLPLLLWNHHPNDG-DDDPKDIHRSSAFRPNQRDAEEGNGL 184
            +  KKR+   D  + FR+TLPLLLWN HPNDG DDDP D H +S+   +  + EE NGL
Sbjct: 125 SINHKKRSRHLDSPATFRQTLPLLLWNDHPNDGDDDDPNDPHSASS-NDSDEEEEEENGL 184

Query: 185 VGWPPLKKRRKRLFMEKRGRGRGRGRGPAAENRRP-VENGGGFYRGSNSSTSNSRYVKVK 244
           VGWPPLKKRRK LFM++       G G  A  RRP V+N G F+RG +   +NSRYVKVK
Sbjct: 185 VGWPPLKKRRKSLFMKE-------GVGRRAVIRRPAVKNDGVFWRGLD--LNNSRYVKVK 244

Query: 245 MEGVGIARKVDLSLHHSFAALTATLMNMFDETDSADAYRLTYQDIDGDWLLAQDVTWR 298
           MEGVGIARKVDL  HHSF AL ATLM MFDET+S + Y+LT+Q+  G+WLLA++VTWR
Sbjct: 245 MEGVGIARKVDLREHHSFDALRATLMKMFDETNS-EGYKLTFQNTKGEWLLAENVTWR 284

[Close]

BLAST of MS003724 vs. NCBI nr
Match: XP_038879327.1 (auxin-responsive protein IAA29-like [Benincasa hispida])

HSP 1 Score: 250.4 bits (638), Expect = 2.0e-62
Identity = 142/215 (66.05%), Postives = 159/215 (73.95%), Query Frame = 0


Query: 88  MAMELQLGLALIPT---KGFDLNTHVHDT-APSFENLDPVCAKKRALDFDPCSAFRRTLP 147
           MAMELQLGLAL PT   K FDLNTH ++   P   + D +  KKR+ DF+  +AFRRTLP
Sbjct: 1   MAMELQLGLALTPTNPIKPFDLNTHHNNPHNPLCFDFDSITHKKRSRDFNHSAAFRRTLP 60

Query: 148 LLLWNHHPNDGDDDPKDIHRSSAFRPNQRDAEEGNGLVGWPPLKKRRKRLFMEKRGRGRG 207
           LLLWN HPNDGDDD  +   S++   +  + E  NGLVGWPPLKKRRK LFM+K      
Sbjct: 61  LLLWNDHPNDGDDDDSNDPHSASSNDSDEEEERENGLVGWPPLKKRRKSLFMKKH----- 120

Query: 208 RGRGPAAENRRP-VENGGGFYRGSNSSTSNSRYVKVKMEGVGIARKVDLSLHHSFAALTA 267
              G AA  RRP VENGGGFYRG NS  + SRYVKVKMEGVGIARKVDLS HHSF AL A
Sbjct: 121 --VGRAAVIRRPMVENGGGFYRGLNS--NYSRYVKVKMEGVGIARKVDLSQHHSFDALRA 180

Query: 268 TLMNMFDETDSADAYRLTYQDIDGDWLLAQDVTWR 298
           TLMNMFDETDS D YRLTYQ+IDGDWLLA++VTWR
Sbjct: 181 TLMNMFDETDS-DDYRLTYQNIDGDWLLAENVTWR 205

[Close]

BLAST of MS003724 vs. NCBI nr
Match: XP_022960765.1 (uncharacterized protein LOC111461467 isoform X2 [Cucurbita moschata])

HSP 1 Score: 234.2 bits (596), Expect = 1.5e-57
Identity = 134/214 (62.62%), Postives = 151/214 (70.56%), Query Frame = 0


Query: 88  MAMELQLGLALI---PTKGFDLNTHVHDTAPSFENLDPVCAKKRALDFDPCSAFRRTLPL 147
           MAMELQL LAL    PTK FDLNTH H   P  +  D +  KKR LD DP SAFRRTLPL
Sbjct: 1   MAMELQLSLALFPTNPTKPFDLNTH-HRALPCLD-FDSISRKKRPLDIDPSSAFRRTLPL 60

Query: 148 LLWNHHPNDGDDDPKDIHRSSAFRPNQRDAEEGNGLVGWPPLKKRRKRLFMEKRGRGRGR 207
           LLWN HPN+GDDDPK+ + +S+  P+  + +E   LVGWPPLKKRRK LF++KR      
Sbjct: 61  LLWNDHPNNGDDDPKNPNSASSNHPDNPEEQEIE-LVGWPPLKKRRKTLFVQKR------ 120

Query: 208 GRGPAAENRRP-VENGGGFYRGSNSSTSNSRYVKVKMEGVGIARKVDLSLHHSFAALTAT 267
             G A   RRP +EN GG YR  NS  +NSRYVKVKMEGVGI RKVD+  HH   AL AT
Sbjct: 121 -IGRAVAIRRPAMENDGGVYRELNS--NNSRYVKVKMEGVGIGRKVDIRQHHCLDALRAT 180

Query: 268 LMNMFDETDSADAYRLTYQDIDGDWLLAQDVTWR 298
           LMNMFD+ DS D Y LTYQD DGDWLLA+DVTWR
Sbjct: 181 LMNMFDKPDS-DGYNLTYQDTDGDWLLAEDVTWR 201

[Close]

BLAST of MS003724 vs. ExPASy Swiss-Prot
Match: Q93WC4 (Auxin-responsive protein IAA29 OS=Arabidopsis thaliana OX=3702 GN=IAA29 PE=1 SV=2)

HSP 1 Score: 97.1 bits (240), Expect = 3.7e-19
Identity = 79/237 (33.33%), Postives = 107/237 (45.15%), Query Frame = 0


Query: 90  MELQLGLALIPTK------GFDLNTHVHDTAPSFENLDPVCAKKRALDFDPC-------- 149
           MEL LGL+L P K       FDLN H      +       C     L F+          
Sbjct: 1   MELDLGLSLSPHKSSKLGFNFDLNKHCAIEGAA------SCLGTEKLRFEATFGLGNVEE 60

Query: 150 SAFRRTLPLLLWNHHPNDGDDDPKDIHRSSAFRPNQRDAEEGNGLVGWPPLKK------- 209
           + +     L   N  PN+ D+DP +   S  +     D EE + +VGWPP+K        
Sbjct: 61  NCYMPKQRLFALNGQPNEEDEDPLESESSIVY----DDEEENSEVVGWPPVKTCMIKYGS 120

Query: 210 -RRKRLFMEKRGRGRGRGRGPAAENR---RPVENGGGFYRGSNSSTSNSRYVKVKMEGVG 269
              + +          RGR   A N     P     G    S+ S+ +S YVKVKM+GV 
Sbjct: 121 YHHRHIRNHHHCPYHHRGRRITAMNNNISNPTTATVGSSSSSSISSRSSMYVKVKMDGVA 180

Query: 270 IARKVDLSLHHSFAALTATLMNMFDETDSADA----YRLTYQDIDGDWLLAQDVTWR 298
           IARKVD+ L +S+ +LT +L+ MF E +  D     Y  T+Q  +GDWLL  DVTW+
Sbjct: 181 IARKVDIKLFNSYESLTNSLITMFTEYEDCDREDTNYTFTFQGKEGDWLLRGDVTWK 227

[Close]

BLAST of MS003724 vs. ExPASy Swiss-Prot
Match: Q5VRR0 (Auxin-responsive protein IAA20 OS=Oryza sativa subsp. japonica OX=39947 GN=IAA20 PE=2 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 6.3e-11
Identity = 66/218 (30.28%), Postives = 86/218 (39.45%), Query Frame = 0


Query: 90  MELQLGLALIPTKGFDLNTHVHDTAPSFENLDPVCAKKRALDFDPCSAFRRTLPLLLWNH 149
           MEL+LGL L           +   +PS        ++   L+  P S  +R     L   
Sbjct: 1   MELELGLRLA----------LPSPSPSPATATAAGSELDLLNSAPGSCRKRGFEEALGGF 60

Query: 150 HPNDGDDDPKDIHRSSAFRPNQRDAEEGN---GLVGWPPLKKRRKRLFMEKRGRGRGRGR 209
             +D +DD      +        D E GN    LVGWPP+K                   
Sbjct: 61  KTDDDNDD-----GNGRGGDGDSDGEMGNKRRKLVGWPPVK------------------- 120

Query: 210 GPAAENRRPVENGGGFYRGSNSSTSNSRYVKVKMEGVGIARKVDLSLHHSFAALTATLMN 269
                 RR    GGG             YVKVKMEG+ I RK+DLS+  S+A L  TL  
Sbjct: 121 --CLHRRRDGGCGGG-------------YVKVKMEGLAIGRKLDLSILGSYAELLDTLHL 169

Query: 270 MFDETDSADA--------YRLTYQDIDGDWLLAQDVTW 297
           MF  T+  D         Y +TY+D +GDW+   DV W
Sbjct: 181 MFPSTNQEDGHDRRRRHPYAVTYEDGEGDWMQVGDVPW 169

[Close]

BLAST of MS003724 vs. ExPASy Swiss-Prot
Match: Q6K846 (Auxin-responsive protein IAA9 OS=Oryza sativa subsp. japonica OX=39947 GN=IAA9 PE=2 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 7.7e-09
Identity = 35/77 (45.45%), Postives = 42/77 (54.55%), Query Frame = 0


Query: 233 SRYVKVKMEGVGIARKVDLSLHHSFAALTATLMNMF-------------DETDSADAYRL 292
           + YVKVK EG  I RKVDL+LH S+  L ATL  MF             D+   A    +
Sbjct: 92  ANYVKVKKEGDAIGRKVDLALHSSYDELAATLARMFPTNDHQGEKKMANDDHGDAAGPVV 151

Query: 293 TYQDIDGDWLLAQDVTW 297
           TY+D DGDW+L  DV W
Sbjct: 152 TYEDGDGDWMLVGDVPW 168

[Close]

BLAST of MS003724 vs. ExPASy Swiss-Prot
Match: Q38831 (Auxin-responsive protein IAA13 OS=Arabidopsis thaliana OX=3702 GN=IAA13 PE=1 SV=2)

HSP 1 Score: 61.2 bits (147), Expect = 2.2e-08
Identity = 43/151 (28.48%), Postives = 63/151 (41.72%), Query Frame = 0


Query: 165 SAFRPNQRDAEEGNGLVGWPPLKKRRKRLFMEKRGRGRGRGRGPAAENRRPVENGGGFYR 224
           SA           + +VGWPP+   R    +  +     R    A + +   +      +
Sbjct: 62  SASHAGSSPPRSSSQVVGWPPIGSHRMNSLVNNQATKSAREEEEAGKKKVKDDEPKDVTK 121

Query: 225 GSNSSTSNSRYVKVKMEGVGIARKVDLSLHHSFAALTATLMNMFDET------------- 284
             N       ++KV M+GV I RKVDL+ H S+  L  TL +MF  T             
Sbjct: 122 KVNGKVQVG-FIKVNMDGVAIGRKVDLNAHSSYENLAQTLEDMFFRTNPGTVGLTSQFTK 181

Query: 285 -----DSADAYRLTYQDIDGDWLLAQDVTWR 298
                D +  + LTY+D +GDW+L  DV WR
Sbjct: 182 PLRLLDGSSEFVLTYEDKEGDWMLVGDVPWR 211

[Close]

BLAST of MS003724 vs. ExPASy Swiss-Prot
Match: A2XB18 (Auxin-responsive protein IAA10 OS=Oryza sativa subsp. indica OX=39946 GN=IAA10 PE=2 SV=2)

HSP 1 Score: 60.1 bits (144), Expect = 5.0e-08
Identity = 42/138 (30.43%), Postives = 62/138 (44.93%), Query Frame = 0


Query: 179 GLVGWPPLKK-RRKRLFMEKRGRGRGRGRGPAAENRRPVENGGGFYRGSNSSTSNSR--- 238
           G+VGWPP+++ R   LF       + +      + ++   N     +         R   
Sbjct: 111 GVVGWPPIRQFRMNSLF------NQAKENTSETDTKKTATNESDVQKDKEEGEKKGRVAG 170

Query: 239 YVKVKMEGVGIARKVDLSLHHSFAALTATLMNMFDE----------------TDSADAYR 297
           +VKV M+G  I RKVDL+ H S+  L   L  MF +                 D++  Y+
Sbjct: 171 WVKVNMDGEVIGRKVDLNAHRSYKTLALALELMFTKPSIGLCASHNTNSLKLLDNSAEYQ 230

[Close]

BLAST of MS003724 vs. ExPASy TrEMBL
Match: A0A6J1CTH1 (Auxin-responsive protein OS=Momordica charantia OX=3673 GN=LOC111014437 PE=3 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 1.4e-114
Identity = 207/212 (97.64%), Postives = 209/212 (98.58%), Query Frame = 0


Query: 88  MAMELQLGLALIPTKGFDLNTHVHDTAPSFENLDPVCAKKRALDFDPCSAFRRTLPLLLW 147
           MAMELQLGLALIPTKGFDLNTHVH+TAPSFE LDPVCAKKRALDFDPCSAFRRTLPLLLW
Sbjct: 1   MAMELQLGLALIPTKGFDLNTHVHETAPSFEYLDPVCAKKRALDFDPCSAFRRTLPLLLW 60

Query: 148 NHHPNDGDDDPKDIHRSSAFRPNQRDAEEGNGLVGWPPLKKRRKRLFMEKR--GRGRGRG 207
           NHHPNDGDDDPKDIHRSSAFRPN+RDAEEGNGLVGWPPLKKRRKRLFMEKR  GRGRGRG
Sbjct: 61  NHHPNDGDDDPKDIHRSSAFRPNRRDAEEGNGLVGWPPLKKRRKRLFMEKRGHGRGRGRG 120

Query: 208 RGPAAENRRPVENGGGFYRGSNSSTSNSRYVKVKMEGVGIARKVDLSLHHSFAALTATLM 267
           RGPAAENRRPVENGGGFYRGSNSSTSNSRYVKVKMEGVGIARKVDLSLHHSFAALTATLM
Sbjct: 121 RGPAAENRRPVENGGGFYRGSNSSTSNSRYVKVKMEGVGIARKVDLSLHHSFAALTATLM 180

Query: 268 NMFDETDSADAYRLTYQDIDGDWLLAQDVTWR 298
           NMFDETDSADAYRLTYQDIDGDWLLAQDVTWR
Sbjct: 181 NMFDETDSADAYRLTYQDIDGDWLLAQDVTWR 212

[Close]

BLAST of MS003724 vs. ExPASy TrEMBL
Match: A0A6J1JF88 (Auxin-responsive protein OS=Cucurbita maxima OX=3661 GN=LOC111485214 PE=3 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 2.3e-72
Identity = 176/301 (58.47%), Postives = 195/301 (64.78%), Query Frame = 0


Query: 6   KEQQGNWVGQSQSIDTDILDVFRLCLQHFVP-----PLSDSCPTPARTSPFTIKPKPPPY 65
           KEQQG+WVGQ QSIDTDI D FRL +QHFVP     PL   C T AR S    KP PPP+
Sbjct: 6   KEQQGSWVGQRQSIDTDISDHFRLSIQHFVPIHILVPLPHVC-THARPSSAPFKP-PPPF 65

Query: 66  HQTHISSPISIPFFVLLLFPFPFYLTFMAMELQLGLALI---PTKGFDLNTHVHDTAPSF 125
                SSP +         P    +  MAMELQLGLAL    PTK F LNTH H   P  
Sbjct: 66  FP---SSPTN---------PHVSSIPSMAMELQLGLALFPTNPTKPFHLNTH-HRALPCL 125

Query: 126 ENLDPVCAKKRALDFDPCSAFRRTLPLLLWNHHPNDGDDDPKDIHRSSAFRPNQRDAEEG 185
           +  D +  KKR LD DP SAFRRTLPLLLWN HPNDGDDDP D + +S+  P+    +E 
Sbjct: 126 D-FDSISGKKRPLDIDPSSAFRRTLPLLLWNDHPNDGDDDPNDPNSASSNHPDNPKEQEI 185

Query: 186 NGLVGWPPLKKRRKRLFMEKRGRGRGRGRGPAAENRRP-VENGGGFYRGSNSSTSNSRYV 245
             LVGWPPLKKRRK LF++KR R        A   RRP +ENGGG +R  NS  +NS YV
Sbjct: 186 E-LVGWPPLKKRRKTLFVQKRIR-------RAVVIRRPAMENGGGVHRELNS--NNSLYV 245

Query: 246 KVKMEGVGIARKVDLSLHHSFAALTATLMNMFDETDSADAYRLTYQDIDGDWLLAQDVTW 298
           KVKMEGVGI RKVD+  HH F AL ATLMNMFD+ DS D Y LTYQD DGDWLLA DVTW
Sbjct: 246 KVKMEGVGIGRKVDIRQHHCFDALRATLMNMFDKPDS-DGYNLTYQDTDGDWLLADDVTW 279

[Close]

BLAST of MS003724 vs. ExPASy TrEMBL
Match: A0A0A0LWC5 (Auxin-responsive protein OS=Cucumis sativus OX=3659 GN=Csa_1G231530 PE=3 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 4.3e-71
Identity = 166/298 (55.70%), Postives = 200/298 (67.11%), Query Frame = 0


Query: 5   KKEQQGNWVGQSQSIDTDILDVFRLCLQHFVPPLSDSCPTPARTSPFTIKPKPPPYHQTH 64
           +KE + NWVGQ QSIDTDILDVF    QHFVP +   CPTPART+P  +    P  +  +
Sbjct: 5   EKEHESNWVGQRQSIDTDILDVFPFSFQHFVPTILILCPTPARTTPLLLLNPHPLSNFHN 64

Query: 65  ISSPISIPFFVLLLFPFPFYLTFMAMELQLGLALIPT---KGFDLNTHVHDTAPSFENLD 124
            +S   + F  + LFP+      MAMELQLGLALIPT   K FDLNT  H+  P     D
Sbjct: 65  SNSSFVLTFSSINLFPY-----LMAMELQLGLALIPTNPIKPFDLNT--HNNNPISLVFD 124

Query: 125 PVCAKKRALDFDPCSAFRRTLPLLLWNHHPNDG-DDDPKDIHRSSAFRPNQRDAEEGNGL 184
            +  KKR+   D  + FR+TLPLLLWN HPNDG DDDP D H +S+   +  + EE NGL
Sbjct: 125 SINHKKRSRHLDSPATFRQTLPLLLWNDHPNDGDDDDPNDPHSASS-NDSDEEEEEENGL 184

Query: 185 VGWPPLKKRRKRLFMEKRGRGRGRGRGPAAENRRP-VENGGGFYRGSNSSTSNSRYVKVK 244
           VGWPPLKKRRK LFM++       G G  A  RRP V+N G F+RG +   +NSRYVKVK
Sbjct: 185 VGWPPLKKRRKSLFMKE-------GVGRRAVIRRPAVKNDGVFWRGLD--LNNSRYVKVK 244

Query: 245 MEGVGIARKVDLSLHHSFAALTATLMNMFDETDSADAYRLTYQDIDGDWLLAQDVTWR 298
           MEGVGIARKVDL  HHSF AL ATLM MFDET+S + Y+LT+Q+  G+WLLA++VTWR
Sbjct: 245 MEGVGIARKVDLREHHSFDALRATLMKMFDETNS-EGYKLTFQNTKGEWLLAENVTWR 284

[Close]

BLAST of MS003724 vs. ExPASy TrEMBL
Match: A0A6J1HA19 (Auxin-responsive protein OS=Cucurbita moschata OX=3662 GN=LOC111461467 PE=3 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 7.1e-58
Identity = 134/214 (62.62%), Postives = 151/214 (70.56%), Query Frame = 0


Query: 88  MAMELQLGLALI---PTKGFDLNTHVHDTAPSFENLDPVCAKKRALDFDPCSAFRRTLPL 147
           MAMELQL LAL    PTK FDLNTH H   P  +  D +  KKR LD DP SAFRRTLPL
Sbjct: 1   MAMELQLSLALFPTNPTKPFDLNTH-HRALPCLD-FDSISRKKRPLDIDPSSAFRRTLPL 60

Query: 148 LLWNHHPNDGDDDPKDIHRSSAFRPNQRDAEEGNGLVGWPPLKKRRKRLFMEKRGRGRGR 207
           LLWN HPN+GDDDPK+ + +S+  P+  + +E   LVGWPPLKKRRK LF++KR      
Sbjct: 61  LLWNDHPNNGDDDPKNPNSASSNHPDNPEEQEIE-LVGWPPLKKRRKTLFVQKR------ 120

Query: 208 GRGPAAENRRP-VENGGGFYRGSNSSTSNSRYVKVKMEGVGIARKVDLSLHHSFAALTAT 267
             G A   RRP +EN GG YR  NS  +NSRYVKVKMEGVGI RKVD+  HH   AL AT
Sbjct: 121 -IGRAVAIRRPAMENDGGVYRELNS--NNSRYVKVKMEGVGIGRKVDIRQHHCLDALRAT 180

Query: 268 LMNMFDETDSADAYRLTYQDIDGDWLLAQDVTWR 298
           LMNMFD+ DS D Y LTYQD DGDWLLA+DVTWR
Sbjct: 181 LMNMFDKPDS-DGYNLTYQDTDGDWLLAEDVTWR 201

[Close]

BLAST of MS003724 vs. ExPASy TrEMBL
Match: A0A6J1I2H3 (Auxin-responsive protein OS=Cucurbita maxima OX=3661 GN=LOC111469303 PE=3 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 2.8e-54
Identity = 130/210 (61.90%), Postives = 142/210 (67.62%), Query Frame = 0


Query: 90  MELQLGLALIPTKGFDLNTH--VHDTAPSFENLDPVCAKKRALDFDPCSAFRRTLPLLLW 149
           MELQLGL+LIP K FDLN    +HDTA S  +L     KKR L  DP SAFRRTLPLLLW
Sbjct: 1   MELQLGLSLIPAKPFDLNNSLLLHDTA-SCLDLTSTIRKKRPLHVDPSSAFRRTLPLLLW 60

Query: 150 NHHPNDGDDDPKDIHRSSAFRPNQRDAEEGNGLVGWPPLKKRRKRLFMEKRGRGRGRGRG 209
           N HPN+GDDD  D H +S       D EE N LVGWPPLKKR+ R  M+KR     RGRG
Sbjct: 61  NDHPNNGDDDDNDPHSASNDSDEDED-EEDNRLVGWPPLKKRKTRSLMQKR-----RGRG 120

Query: 210 PAAENRRPVENGGGFYRGSNSSTSNSRYVKVKMEGVGIARKVDLSLHHSFAALTATLMNM 269
                 RP  NGGG      +  SNSRYVKVKMEGVGI RKVDLS +H F  L ATLM M
Sbjct: 121 ------RPAVNGGGL-----NWNSNSRYVKVKMEGVGIGRKVDLSQYHCFDQLRATLMKM 180

Query: 270 FDETDSADAYRLTYQDIDGDWLLAQDVTWR 298
           FD+ D  D++RLTYQDIDGDWLLA DVTWR
Sbjct: 181 FDKMD-PDSHRLTYQDIDGDWLLADDVTWR 191

[Close]

BLAST of MS003724 vs. TAIR 10
Match: AT4G32280.1 (indole-3-acetic acid inducible 29 )

HSP 1 Score: 97.1 bits (240), Expect = 2.6e-20
Identity = 79/237 (33.33%), Postives = 107/237 (45.15%), Query Frame = 0


Query: 90  MELQLGLALIPTK------GFDLNTHVHDTAPSFENLDPVCAKKRALDFDPC-------- 149
           MEL LGL+L P K       FDLN H      +       C     L F+          
Sbjct: 1   MELDLGLSLSPHKSSKLGFNFDLNKHCAIEGAA------SCLGTEKLRFEATFGLGNVEE 60

Query: 150 SAFRRTLPLLLWNHHPNDGDDDPKDIHRSSAFRPNQRDAEEGNGLVGWPPLKK------- 209
           + +     L   N  PN+ D+DP +   S  +     D EE + +VGWPP+K        
Sbjct: 61  NCYMPKQRLFALNGQPNEEDEDPLESESSIVY----DDEEENSEVVGWPPVKTCMIKYGS 120

Query: 210 -RRKRLFMEKRGRGRGRGRGPAAENR---RPVENGGGFYRGSNSSTSNSRYVKVKMEGVG 269
              + +          RGR   A N     P     G    S+ S+ +S YVKVKM+GV 
Sbjct: 121 YHHRHIRNHHHCPYHHRGRRITAMNNNISNPTTATVGSSSSSSISSRSSMYVKVKMDGVA 180

Query: 270 IARKVDLSLHHSFAALTATLMNMFDETDSADA----YRLTYQDIDGDWLLAQDVTWR 298
           IARKVD+ L +S+ +LT +L+ MF E +  D     Y  T+Q  +GDWLL  DVTW+
Sbjct: 181 IARKVDIKLFNSYESLTNSLITMFTEYEDCDREDTNYTFTFQGKEGDWLLRGDVTWK 227

[Close]

BLAST of MS003724 vs. TAIR 10
Match: AT2G33310.1 (auxin-induced protein 13 )

HSP 1 Score: 61.2 bits (147), Expect = 1.6e-09
Identity = 41/136 (30.15%), Postives = 60/136 (44.12%), Query Frame = 0


Query: 180 LVGWPPLKKRRKRLFMEKRGRGRGRGRGPAAENRRPVENGGGFYRGSNSSTSNSRYVKVK 239
           +VGWPP+   R    +  +     R    A + +   +      +  N       ++KV 
Sbjct: 76  VVGWPPIGSHRMNSLVNNQATKSAREEEEAGKKKVKDDEPKDVTKKVNGKVQVG-FIKVN 135

Query: 240 MEGVGIARKVDLSLHHSFAALTATLMNMFDET------------------DSADAYRLTY 298
           M+GV I RKVDL+ H S+  L  TL +MF  T                  D +  + LTY
Sbjct: 136 MDGVAIGRKVDLNAHSSYENLAQTLEDMFFRTNPGTVGLTSQFTKPLRLLDGSSEFVLTY 195

[Close]

BLAST of MS003724 vs. TAIR 10
Match: AT2G33310.2 (auxin-induced protein 13 )

HSP 1 Score: 61.2 bits (147), Expect = 1.6e-09
Identity = 43/151 (28.48%), Postives = 63/151 (41.72%), Query Frame = 0


Query: 165 SAFRPNQRDAEEGNGLVGWPPLKKRRKRLFMEKRGRGRGRGRGPAAENRRPVENGGGFYR 224
           SA           + +VGWPP+   R    +  +     R    A + +   +      +
Sbjct: 62  SASHAGSSPPRSSSQVVGWPPIGSHRMNSLVNNQATKSAREEEEAGKKKVKDDEPKDVTK 121

Query: 225 GSNSSTSNSRYVKVKMEGVGIARKVDLSLHHSFAALTATLMNMFDET------------- 284
             N       ++KV M+GV I RKVDL+ H S+  L  TL +MF  T             
Sbjct: 122 KVNGKVQVG-FIKVNMDGVAIGRKVDLNAHSSYENLAQTLEDMFFRTNPGTVGLTSQFTK 181

Query: 285 -----DSADAYRLTYQDIDGDWLLAQDVTWR 298
                D +  + LTY+D +GDW+L  DV WR
Sbjct: 182 PLRLLDGSSEFVLTYEDKEGDWMLVGDVPWR 211

[Close]

BLAST of MS003724 vs. TAIR 10
Match: AT2G33310.3 (auxin-induced protein 13 )

HSP 1 Score: 61.2 bits (147), Expect = 1.6e-09
Identity = 41/136 (30.15%), Postives = 60/136 (44.12%), Query Frame = 0


Query: 180 LVGWPPLKKRRKRLFMEKRGRGRGRGRGPAAENRRPVENGGGFYRGSNSSTSNSRYVKVK 239
           +VGWPP+   R    +  +     R    A + +   +      +  N       ++KV 
Sbjct: 76  VVGWPPIGSHRMNSLVNNQATKSAREEEEAGKKKVKDDEPKDVTKKVNGKVQVG-FIKVN 135

Query: 240 MEGVGIARKVDLSLHHSFAALTATLMNMFDET------------------DSADAYRLTY 298
           M+GV I RKVDL+ H S+  L  TL +MF  T                  D +  + LTY
Sbjct: 136 MDGVAIGRKVDLNAHSSYENLAQTLEDMFFRTNPGTVGLTSQFTKPLRLLDGSSEFVLTY 195

[Close]

BLAST of MS003724 vs. TAIR 10
Match: AT1G04550.2 (AUX/IAA transcriptional regulator family protein )

HSP 1 Score: 58.2 bits (139), Expect = 1.3e-08
Identity = 43/135 (31.85%), Postives = 59/135 (43.70%), Query Frame = 0


Query: 180 LVGWPPLKKRRKRLFMEKRGRGRGRGRGPAAENRRPVENG--GGFYRGSNSSTSNSRYVK 239
           +VGWPP+   R    +  +     R      E ++ V+N          N       +VK
Sbjct: 70  VVGWPPIGLHRMNSLVNNQAMKAARAEEGDGE-KKVVKNDELKDVSMKVNPKVQGLGFVK 129

Query: 240 VKMEGVGIARKVDLSLHHSFAALTATLMNMF---------------DETDSADAYRLTYQ 298
           V M+GVGI RKVD+  H S+  L  TL  MF                  D +  + LTY+
Sbjct: 130 VNMDGVGIGRKVDMRAHSSYENLAQTLEEMFFGMTGTTCREKVKPLRLLDGSSDFVLTYE 189

The following BLAST results are available for this feature:

BLAST of MS003724 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022144854.1	2.9e-114	97.64	auxin-responsive protein IAA29-like [Momordica charantia]	[more]
XP_022987761.1	4.7e-72	58.47	uncharacterized protein LOC111485214 isoform X2 [Cucurbita maxima]	[more]
KGN65132.1	8.9e-71	55.70	hypothetical protein Csa_023489 [Cucumis sativus]	[more]
XP_038879327.1	2.0e-62	66.05	auxin-responsive protein IAA29-like [Benincasa hispida]	[more]
XP_022960765.1	1.5e-57	62.62	uncharacterized protein LOC111461467 isoform X2 [Cucurbita moschata]	[more]

BLAST of MS003724 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93WC4	3.7e-19	33.33	Auxin-responsive protein IAA29 OS=Arabidopsis thaliana OX=3702 GN=IAA29 PE=1 SV=...	[more]
Q5VRR0	6.3e-11	30.28	Auxin-responsive protein IAA20 OS=Oryza sativa subsp. japonica OX=39947 GN=IAA20...	[more]
Q6K846	7.7e-09	45.45	Auxin-responsive protein IAA9 OS=Oryza sativa subsp. japonica OX=39947 GN=IAA9 P...	[more]
Q38831	2.2e-08	28.48	Auxin-responsive protein IAA13 OS=Arabidopsis thaliana OX=3702 GN=IAA13 PE=1 SV=...	[more]
A2XB18	5.0e-08	30.43	Auxin-responsive protein IAA10 OS=Oryza sativa subsp. indica OX=39946 GN=IAA10 P...	[more]

BLAST of MS003724 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CTH1	1.4e-114	97.64	Auxin-responsive protein OS=Momordica charantia OX=3673 GN=LOC111014437 PE=3 SV=...	[more]
A0A6J1JF88	2.3e-72	58.47	Auxin-responsive protein OS=Cucurbita maxima OX=3661 GN=LOC111485214 PE=3 SV=1	[more]
A0A0A0LWC5	4.3e-71	55.70	Auxin-responsive protein OS=Cucumis sativus OX=3659 GN=Csa_1G231530 PE=3 SV=1	[more]
A0A6J1HA19	7.1e-58	62.62	Auxin-responsive protein OS=Cucurbita moschata OX=3662 GN=LOC111461467 PE=3 SV=1	[more]
A0A6J1I2H3	2.8e-54	61.90	Auxin-responsive protein OS=Cucurbita maxima OX=3661 GN=LOC111469303 PE=3 SV=1	[more]

BLAST of MS003724 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G32280.1	2.6e-20	33.33	indole-3-acetic acid inducible 29	[more]
AT2G33310.1	1.6e-09	30.15	auxin-induced protein 13	[more]
AT2G33310.2	1.6e-09	28.48	auxin-induced protein 13	[more]
AT2G33310.3	1.6e-09	30.15	auxin-induced protein 13	[more]
AT1G04550.2	1.3e-08	31.85	AUX/IAA transcriptional regulator family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.20.90		coord: 227..297 e-value: 6.4E-18 score: 66.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 153..170
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 199..228
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 153..182
None	No IPR available	PANTHER	PTHR31734:SF44	AUXIN-RESPONSIVE PROTEIN IAA29	coord: 90..297
None	No IPR available	SUPERFAMILY	54277	CAD & PB1 domains	coord: 227..296
IPR033389	AUX/IAA domain	PFAM	PF02309	AUX_IAA	coord: 170..270 e-value: 7.9E-15 score: 55.4
IPR003311	AUX/IAA protein	PANTHER	PTHR31734	AUXIN-RESPONSIVE PROTEIN IAA17	coord: 90..297
IPR000270	PB1 domain	PROSITE	PS51745	PB1	coord: 233..297 score: 19.016188

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS003724.1	MS003724.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005515	protein binding