MS003431 (gene) Bitter gourd (TR) v1

BLAST of MS003431 vs. NCBI nr
Match: XP_022134458.1 (rop guanine nucleotide exchange factor 5 [Momordica charantia])

HSP 1 Score: 1156.0 bits (2989), Expect = 0.0e+00
Identity = 596/602 (99.00%), Postives = 598/602 (99.34%), Query Frame = 0

Query: 1   MEASVKRSENARKIGGSFGSNGVGTESSLTESSAASGESSSSRSSSDTSGDDVKAGECSS 60
           MEASVKRSENA KI GSFGSNGVGTESSLTESSAASGESSSSRSSSDTSGDDVKAGECSS
Sbjct: 1   MEASVKRSENAPKISGSFGSNGVGTESSLTESSAASGESSSSRSSSDTSGDDVKAGECSS 60

Query: 61  PPQLGWPIRKAQLCKSSNSKVREVEQKPNSSDSKLSKTAPKVSEMEMMKERFSKLLLGED 120
           PPQLGWPIRKAQLCKSSNSKVREVEQKPNSSDSKLSKTAPKVSEMEMMKERFSKLLLGED
Sbjct: 61  PPQLGWPIRKAQLCKSSNSKVREVEQKPNSSDSKLSKTAPKVSEMEMMKERFSKLLLGED 120

Query: 121 MSGSGKGVCTALAISNAITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLCVSDHIVEL 180
           MSGSGKGVCTALAISNAITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLCVSDHIVEL
Sbjct: 121 MSGSGKGVCTALAISNAITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLCVSDHIVEL 180

Query: 181 IPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTEFWYVDQGIVAS 240
           IPSFQTFPDGSKLEVMTSR RSDIFINLPALRKLDNMLLEILESFVDTEFWYVDQGIVAS
Sbjct: 181 IPSFQTFPDGSKLEVMTSRSRSDIFINLPALRKLDNMLLEILESFVDTEFWYVDQGIVAS 240

Query: 241 DGDGSSSFRKIVQRQQEKWWLPVPRVPAGGLGEDSRKQLHHTRDCTNQILKAVVAINNIA 300
           DGDGSSSFRKIVQRQQEKWWLPVPRVPAGGLGEDSRKQLHHTRDCTNQILKAVVAINNIA
Sbjct: 241 DGDGSSSFRKIVQRQQEKWWLPVPRVPAGGLGEDSRKQLHHTRDCTNQILKAVVAINNIA 300

Query: 301 LADMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVANRVE 360
           LADMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVANRVE
Sbjct: 301 LADMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVANRVE 360

Query: 361 AAIYVWRRRAHSKPQINPTRSTARSSWEMVKDLMIDGDKREFLAERAEGLLHSLKQRFPS 420
           AAIYVWRRRAHSKPQINPTRSTARSSWEMVKDLMIDGDKREFLAERAEGLLHSLKQRFPS
Sbjct: 361 AAIYVWRRRAHSKPQINPTRSTARSSWEMVKDLMIDGDKREFLAERAEGLLHSLKQRFPS 420

Query: 421 LTQTTLDTSKIQFNKDVGKSILESYSRVLESLAFNIIARIDDLLYVDDLTKHSDRLTSVG 480
           LTQTTLDTSKIQFNKDVGKSILESYSRVLESLAFNIIARIDDLLYVDDLTKHSDRLTSVG
Sbjct: 421 LTQTTLDTSKIQFNKDVGKSILESYSRVLESLAFNIIARIDDLLYVDDLTKHSDRLTSVG 480

Query: 481 TVNVVGHKKAAYSVHLSSTPYKLTATTPSFSPAPLVSPAKSERMPFLNNRNVGKPIRRGF 540
           TVNVVGHKKAAYSVHLSSTPYKLTATTPSFSPAPLVSPAKSERMPFLNNRNVGKPIRRGF
Sbjct: 481 TVNVVGHKKAAYSVHLSSTPYKLTATTPSFSPAPLVSPAKSERMPFLNNRNVGKPIRRGF 540

Query: 541 GVKRALTNYLGVETKAKTCLNSIEGPASIQNIVVNESSERRKSSSADHSVVSCSKLLADK 600
           GVKRALTNYLGVETKAKTCLNSIEGPASIQNIVVNESSERRKSSSAD SVVSCSKLL+DK
Sbjct: 541 GVKRALTNYLGVETKAKTCLNSIEGPASIQNIVVNESSERRKSSSADQSVVSCSKLLSDK 600

Query: 601 VK 603
           +K
Sbjct: 601 IK 602

BLAST of MS003431 vs. NCBI nr
Match: XP_038884428.1 (rop guanine nucleotide exchange factor 5 [Benincasa hispida])

HSP 1 Score: 1072.4 bits (2772), Expect = 1.4e-309
Identity = 554/603 (91.87%), Postives = 576/603 (95.52%), Query Frame = 0

Query: 1   MEASVKRSENARKIGGSFGSNGVGTESSLTESSAASGESSSSRSSSDTSGDDVKAGECSS 60
           M ASVK +ENAR+IG SFGSNGVG E S TESSA SGESSSSRSSSD S     AGECSS
Sbjct: 1   MAASVKTAENAREIGASFGSNGVGIE-SFTESSAVSGESSSSRSSSDNS-----AGECSS 60

Query: 61  PPQLGWPIRKAQLCKSSNSKVREVEQKPNSSDSKL-SKTAPKVSEMEMMKERFSKLLLGE 120
           PPQLGWPIRKAQLCKSSNSKVREVEQKPNS+DSKL +KT PKVSEMEMMKERF+KLLLGE
Sbjct: 61  PPQLGWPIRKAQLCKSSNSKVREVEQKPNSNDSKLNNKTVPKVSEMEMMKERFAKLLLGE 120

Query: 121 DMSGSGKGVCTALAISNAITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLCVSDHIVE 180
           DMSGSGKGV TALAISN+ITNLCAT+FGQLWRLEPLPKEKKSMW+RE+EWLLCVSDHIVE
Sbjct: 121 DMSGSGKGVSTALAISNSITNLCATVFGQLWRLEPLPKEKKSMWKREMEWLLCVSDHIVE 180

Query: 181 LIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTEFWYVDQGIVA 240
           LIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESF DTEFWYVDQGIVA
Sbjct: 181 LIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFADTEFWYVDQGIVA 240

Query: 241 SDGDGSSSFRKIVQRQQEKWWLPVPRVPAGGLGEDSRKQLHHTRDCTNQILKAVVAINNI 300
           SDGDGSSSFRKIVQRQQEKWWLPVPRVPAGGLGEDSRKQLHHTRDCTNQILKAV+AINNI
Sbjct: 241 SDGDGSSSFRKIVQRQQEKWWLPVPRVPAGGLGEDSRKQLHHTRDCTNQILKAVMAINNI 300

Query: 301 ALADMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVANRV 360
           AL DMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVANRV
Sbjct: 301 ALNDMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVANRV 360

Query: 361 EAAIYVWRRRAHSKPQINPTRSTARSSWEMVKDLMIDGDKREFLAERAEGLLHSLKQRFP 420
           EAAIYVWRRRAHSKPQINPTRSTARSSWEMVKDLMIDGDKREFLAERAEGLL+SLKQRFP
Sbjct: 361 EAAIYVWRRRAHSKPQINPTRSTARSSWEMVKDLMIDGDKREFLAERAEGLLNSLKQRFP 420

Query: 421 SLTQTTLDTSKIQFNKDVGKSILESYSRVLESLAFNIIARIDDLLYVDDLTKHSDRLTSV 480
           SLTQTTLDTSKIQFNKDVGKSILESYSRVLESLAFNIIARIDDLLYVDDLTKHS+RLTS 
Sbjct: 421 SLTQTTLDTSKIQFNKDVGKSILESYSRVLESLAFNIIARIDDLLYVDDLTKHSNRLTSA 480

Query: 481 GTVNVVGHKKAAYSVHLSSTPYKLTATTPSFSPAPLVSPAKSERMPFLNNRNVGKPIRRG 540
            TVNVVGHKKAAYSVHLSSTPYKLT+TTPSFSPAPLVSPAK+ER+PFLNN+N  KP RRG
Sbjct: 481 ATVNVVGHKKAAYSVHLSSTPYKLTSTTPSFSPAPLVSPAKTERVPFLNNKNTSKPPRRG 540

Query: 541 FGVKRALTNYLGVETKAKTCLNSIEGPASIQNIVVNESSERRKSSSADHSVVSCSKLLAD 600
           FGVKRALTNYLGVETK KTC NSIEG ASIQNI+VNE+SE+RKSSSAD SV+SCSKLLA+
Sbjct: 541 FGVKRALTNYLGVETKLKTCTNSIEGAASIQNIIVNENSEQRKSSSADQSVISCSKLLAE 597

Query: 601 KVK 603
           K+K
Sbjct: 601 KIK 597

BLAST of MS003431 vs. NCBI nr
Match: XP_004138576.1 (rop guanine nucleotide exchange factor 5 [Cucumis sativus] >KGN45755.1 hypothetical protein Csa_004850 [Cucumis sativus])

HSP 1 Score: 1057.7 bits (2734), Expect = 3.6e-305
Identity = 546/602 (90.70%), Postives = 570/602 (94.68%), Query Frame = 0

Query: 1   MEASVKRSENARKIGGSFGSNGVGTESSLTESSAASGESSSSRSSSDTSGDDVKAGECSS 60
           MEASV  ++NARKI  SFGS G GTE   TESSA SG+SSSSRSSSD S     AGECSS
Sbjct: 1   MEASVNTTDNARKIATSFGSIGAGTE-LFTESSAVSGQSSSSRSSSDNS-----AGECSS 60

Query: 61  PPQLGWPIRKAQLCKSSNSKVREVEQKPNSSDSKLSKTAPKVSEMEMMKERFSKLLLGED 120
           PPQLGWPIRKAQLCKSSNS+VREVEQKP+S+DSK SKT  KVSEME+MKERF+KLLLGED
Sbjct: 61  PPQLGWPIRKAQLCKSSNSRVREVEQKPSSTDSKPSKTVTKVSEMELMKERFAKLLLGED 120

Query: 121 MSGSGKGVCTALAISNAITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLCVSDHIVEL 180
           MSGSGKGV TALAISN+ITNLCATIFGQLWRLEPLPKEKKSMW+RE+EWLLCVSDHIVEL
Sbjct: 121 MSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKKSMWKRELEWLLCVSDHIVEL 180

Query: 181 IPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTEFWYVDQGIVAS 240
           IPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESF DTEFWYVDQGIV+S
Sbjct: 181 IPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFADTEFWYVDQGIVSS 240

Query: 241 DGDGSSSFRKIVQRQQEKWWLPVPRVPAGGLGEDSRKQLHHTRDCTNQILKAVVAINNIA 300
           DGDGSSSFRKIVQRQQEKWWLPVPRVPAGGLGEDSRKQLHHTRDCTNQILKAV+AINNIA
Sbjct: 241 DGDGSSSFRKIVQRQQEKWWLPVPRVPAGGLGEDSRKQLHHTRDCTNQILKAVMAINNIA 300

Query: 301 LADMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVANRVE 360
           L DMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVANRVE
Sbjct: 301 LNDMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVANRVE 360

Query: 361 AAIYVWRRRAHSKPQINPTRSTARSSWEMVKDLMIDGDKREFLAERAEGLLHSLKQRFPS 420
           AAIYVWRRRAHSKPQINPTRSTARSSWEMVKDLMIDGDKREFLAERAEGLLHSLKQRFPS
Sbjct: 361 AAIYVWRRRAHSKPQINPTRSTARSSWEMVKDLMIDGDKREFLAERAEGLLHSLKQRFPS 420

Query: 421 LTQTTLDTSKIQFNKDVGKSILESYSRVLESLAFNIIARIDDLLYVDDLTKHSDRLTSVG 480
           LTQTTLDTSKIQFNKDVGKSILESYSRVLESLAFNIIARIDDLLYVDDLTKHS+RLTS  
Sbjct: 421 LTQTTLDTSKIQFNKDVGKSILESYSRVLESLAFNIIARIDDLLYVDDLTKHSNRLTSAA 480

Query: 481 TVNVVGHKKAAYSVHLSSTPYKLTATTPSFSPAPLVSPAKSERMPFLNNRNVGKPIRRGF 540
           TVNVVGHKKAAYSVHLSSTPYKLT+TTPSFSPAPLVSPAK+ER+PFLNN+   KP RRGF
Sbjct: 481 TVNVVGHKKAAYSVHLSSTPYKLTSTTPSFSPAPLVSPAKTERVPFLNNKITSKPPRRGF 540

Query: 541 GVKRALTNYLGVETKAKTCLNSIEGPASIQNIVVNESSERRKSSSADHSVVSCSKLLADK 600
           GVKRALTNYLGVETK KTC NSIEG ASIQNI+VNE+SE+RKSSSAD SV+SCSKLLA+K
Sbjct: 541 GVKRALTNYLGVETKPKTCTNSIEGAASIQNIIVNENSEQRKSSSADQSVISCSKLLAEK 596

Query: 601 VK 603
           +K
Sbjct: 601 MK 596

BLAST of MS003431 vs. NCBI nr
Match: XP_008441412.1 (PREDICTED: rop guanine nucleotide exchange factor 5 [Cucumis melo] >KAA0041473.1 rop guanine nucleotide exchange factor 5 [Cucumis melo var. makuwa] >TYK24347.1 rop guanine nucleotide exchange factor 5 [Cucumis melo var. makuwa])

HSP 1 Score: 1055.0 bits (2727), Expect = 2.3e-304
Identity = 542/602 (90.03%), Postives = 570/602 (94.68%), Query Frame = 0

Query: 1   MEASVKRSENARKIGGSFGSNGVGTESSLTESSAASGESSSSRSSSDTSGDDVKAGECSS 60
           MEASVK ++NARKI  SFGS G GTE   TESSA SG+SSSSRSSSD S     AGECSS
Sbjct: 1   MEASVKTTDNARKIAASFGSIGAGTE-LFTESSAVSGQSSSSRSSSDNS-----AGECSS 60

Query: 61  PPQLGWPIRKAQLCKSSNSKVREVEQKPNSSDSKLSKTAPKVSEMEMMKERFSKLLLGED 120
           PPQLGWPIRKAQLCKSSNS+VREVEQKP+S+DSK SKT  KVSEME+MKERF+KLLLGED
Sbjct: 61  PPQLGWPIRKAQLCKSSNSRVREVEQKPSSNDSKPSKTVTKVSEMELMKERFAKLLLGED 120

Query: 121 MSGSGKGVCTALAISNAITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLCVSDHIVEL 180
           MSGSGKGV TALAISN+ITNLCAT+FGQLWRLEPLPKEKKSMW+RE+EWLLCVSDHIVEL
Sbjct: 121 MSGSGKGVSTALAISNSITNLCATVFGQLWRLEPLPKEKKSMWKRELEWLLCVSDHIVEL 180

Query: 181 IPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTEFWYVDQGIVAS 240
           IPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESF DTEFWYVDQGIV+S
Sbjct: 181 IPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFADTEFWYVDQGIVSS 240

Query: 241 DGDGSSSFRKIVQRQQEKWWLPVPRVPAGGLGEDSRKQLHHTRDCTNQILKAVVAINNIA 300
           DGDGSSSFRKIVQRQQEKWWLPVPRVPAGGL EDSRKQLHHTRDCTNQILKAV+AINNIA
Sbjct: 241 DGDGSSSFRKIVQRQQEKWWLPVPRVPAGGLAEDSRKQLHHTRDCTNQILKAVMAINNIA 300

Query: 301 LADMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVANRVE 360
           L DMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVANRVE
Sbjct: 301 LNDMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVANRVE 360

Query: 361 AAIYVWRRRAHSKPQINPTRSTARSSWEMVKDLMIDGDKREFLAERAEGLLHSLKQRFPS 420
           AAIYVWRRRAHSKPQINPTRSTARSSWEMVKDLMIDGDKREFLAERAEGLLHSLKQRFPS
Sbjct: 361 AAIYVWRRRAHSKPQINPTRSTARSSWEMVKDLMIDGDKREFLAERAEGLLHSLKQRFPS 420

Query: 421 LTQTTLDTSKIQFNKDVGKSILESYSRVLESLAFNIIARIDDLLYVDDLTKHSDRLTSVG 480
           LTQTTLDTSKIQFNKD+GKSILESYSRVLESLA+NIIARIDDLLYVDDLTKHS+RLTS  
Sbjct: 421 LTQTTLDTSKIQFNKDIGKSILESYSRVLESLAYNIIARIDDLLYVDDLTKHSNRLTSAA 480

Query: 481 TVNVVGHKKAAYSVHLSSTPYKLTATTPSFSPAPLVSPAKSERMPFLNNRNVGKPIRRGF 540
           TVNVVGHKKAAYSVHLSSTPYKLT+TTPSFSPAPLVSPAK+ER+PFLNN+   KP RRGF
Sbjct: 481 TVNVVGHKKAAYSVHLSSTPYKLTSTTPSFSPAPLVSPAKTERVPFLNNKITSKPPRRGF 540

Query: 541 GVKRALTNYLGVETKAKTCLNSIEGPASIQNIVVNESSERRKSSSADHSVVSCSKLLADK 600
           GVKRALTNYLGVETK +TC NSIEG ASIQNI+VNE+SE+RKSSSAD SV+SCSKLLA+K
Sbjct: 541 GVKRALTNYLGVETKPRTCTNSIEGAASIQNIIVNENSEQRKSSSADQSVISCSKLLAEK 596

Query: 601 VK 603
           +K
Sbjct: 601 MK 596

BLAST of MS003431 vs. NCBI nr
Match: XP_022993942.1 (rop guanine nucleotide exchange factor 5-like [Cucurbita maxima])

HSP 1 Score: 1020.4 bits (2637), Expect = 6.4e-294
Identity = 529/602 (87.87%), Postives = 555/602 (92.19%), Query Frame = 0

Query: 1   MEASVKRSENARKIGGSFGSNGVGTESSLTESSAASGESSSSRSSSDTSGDDVKAGECSS 60
           ME SVKRSE ARK    FGSNG GTE S TE SA SGESSSSRSSSD+S DD+KA ECSS
Sbjct: 1   MEDSVKRSEKARK----FGSNGAGTE-SFTEFSAVSGESSSSRSSSDSSADDLKAVECSS 60

Query: 61  PPQLGWPIRKAQLCKSSNSKVREVEQKPNSSDSKLSKTAPKVSEMEMMKERFSKLLLGED 120
           PPQLGWPIRKAQ  K+SNS+    EQ  NS+ SK++KT PKVSE+EMMKERF+KLLLGED
Sbjct: 61  PPQLGWPIRKAQPSKTSNSR----EQNSNSNGSKINKTVPKVSELEMMKERFAKLLLGED 120

Query: 121 MSGSGKGVCTALAISNAITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLCVSDHIVEL 180
           MSGSGKGV TALAISN+ITNLCATIFGQLWRLEPLP EKKSMWRRE+EWLLCVS HIVEL
Sbjct: 121 MSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPTEKKSMWRREMEWLLCVSGHIVEL 180

Query: 181 IPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTEFWYVDQGIVAS 240
           IPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESF DTEFWYVDQGIV++
Sbjct: 181 IPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFTDTEFWYVDQGIVSA 240

Query: 241 DGDGSSSFRKIVQRQQEKWWLPVPRVPAGGLGEDSRKQLHHTRDCTNQILKAVVAINNIA 300
           DGDGSSSFRKIVQRQQEKWWLPVPRVP GGLGEDSRKQLHH RDCT+QILKAV AINNIA
Sbjct: 241 DGDGSSSFRKIVQRQQEKWWLPVPRVPVGGLGEDSRKQLHHIRDCTSQILKAVKAINNIA 300

Query: 301 LADMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVANRVE 360
           L DMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSS+HVALDVANRVE
Sbjct: 301 LNDMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSDHVALDVANRVE 360

Query: 361 AAIYVWRRRAHSKPQINPTRSTARSSWEMVKDLMIDGDKREFLAERAEGLLHSLKQRFPS 420
           AAIYVWRRRAHSKPQ+NPTRS+ RSSWEMVKDLMIDGDKREFLAERAEG LHSLKQRFP+
Sbjct: 361 AAIYVWRRRAHSKPQLNPTRSSTRSSWEMVKDLMIDGDKREFLAERAEGFLHSLKQRFPT 420

Query: 421 LTQTTLDTSKIQFNKDVGKSILESYSRVLESLAFNIIARIDDLLYVDDLTKHSDRLTSVG 480
           LTQTTLDTSKIQFNKDVGKSILESYSRVLES+A+NIIARIDDLLYVDDLTKHS RLTSV 
Sbjct: 421 LTQTTLDTSKIQFNKDVGKSILESYSRVLESIAYNIIARIDDLLYVDDLTKHSGRLTSVA 480

Query: 481 TVNVVGHKKAAYSVHLSSTPYKLTATTPSFSPAPLVSPAKSERMPFLNNRNVGKPIRRGF 540
           TVNVVGHKKAAYSVH SSTPYKLT+TTPSFSPAP VSPAK+ER PFLNN N  KP RRGF
Sbjct: 481 TVNVVGHKKAAYSVHFSSTPYKLTSTTPSFSPAPKVSPAKTERPPFLNNNNNSKPPRRGF 540

Query: 541 GVKRALTNYLGVETKAKTCLNSIEGPASIQNIVVNESSERRKSSSADHSVVSCSKLLADK 600
           GVKRALTNYL VETKAKTC NSIEGPASIQNIVVNESSE+RKSSSAD +V++CSKLLAD 
Sbjct: 541 GVKRALTNYLSVETKAKTCTNSIEGPASIQNIVVNESSEQRKSSSADQNVINCSKLLADT 593

Query: 601 VK 603
            K
Sbjct: 601 TK 593

BLAST of MS003431 vs. ExPASy Swiss-Prot
Match: F4K295 (Rop guanine nucleotide exchange factor 5 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF5 PE=2 SV=1)

HSP 1 Score: 650.6 bits (1677), Expect = 1.7e-185
Identity = 354/582 (60.82%), Postives = 430/582 (73.88%), Query Frame = 0

Query: 26  ESSLTESSAASGESSSSRSSSDTSGDDVKAGECSSPPQ--LGWPIRKAQLCKSSNSKVRE 85
           E  LTES   S  S +S  SS T+   V +     PP   LGWPIRKA   K+S   V  
Sbjct: 23  EDILTESKERSSTSGASYESSSTT--TVASSSPPPPPSQILGWPIRKASFRKNSKESVNF 82

Query: 86  VEQKPN-SSDSKLSKTAPKVSEMEMMKERFSKLLLGEDMSGSGKGVCTALAISNAITNLC 145
             +K     DS         +++EMMKERF+KLLLGEDMSGSGKGVCTALAISNAITNLC
Sbjct: 83  DHKKLTLHDDSGFKAKEMNSADVEMMKERFAKLLLGEDMSGSGKGVCTALAISNAITNLC 142

Query: 146 ATIFGQLWRLEPLPKEKKSMWRREIEWLLCVSDHIVELIPSFQTFPDGSKLEVMTSRPRS 205
           ATIFGQLWRLEPL  EKK MWRRE+EW+L VSDHIVEL PS QT+PDG+K EVMT RPR 
Sbjct: 143 ATIFGQLWRLEPLSSEKKEMWRREMEWILSVSDHIVELTPSTQTYPDGNKFEVMTCRPRF 202

Query: 206 DIFINLPALRKLDNMLLEILESFVDTEFWYVDQGIVASDGDGSSSFRKIVQRQQEKWWLP 265
           D+FINLPALRKLDNMLL+IL SF  TEFWYVDQGIVAS+ DGS+SFR+ +QRQ+EKWWLP
Sbjct: 203 DLFINLPALRKLDNMLLDILASFKKTEFWYVDQGIVASENDGSASFRRKIQRQEEKWWLP 262

Query: 266 VPRVPAGGLGEDSRKQLHHTRDCTNQILKAVVAINNIALADMEVPESYLETLPKNGRACL 325
           VPR+   GL E++R +L+H R+C  QILKA +AIN++AL +M+VP+SYLETLPKNGR+CL
Sbjct: 263 VPRLAPNGLTEEARTELNHKRECATQILKAAMAINSLALTEMDVPKSYLETLPKNGRSCL 322

Query: 326 GDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVANRVEAAIYVWRRRAHSKPQINPTRS- 385
           GDVIYRY+TS+ FS+E LLDCLDLSSEH+ALD+ANRVEA+IYVWRRR  +K  +N   S 
Sbjct: 323 GDVIYRYVTSDKFSAESLLDCLDLSSEHIALDIANRVEASIYVWRRRVQTKLGVNNNTSS 382

Query: 386 -TARSSWEMVKDLMIDGDKREFLAERAEGLLHSLKQRFPSLTQTTLDTSKIQFNKDVGKS 445
            T + +WEMVK+LM  GDKR  L ER+E LL  LKQRFPSLTQT+LD SKIQ+NKD+GKS
Sbjct: 383 TTPKLTWEMVKELMAAGDKRGLLVERSETLLRCLKQRFPSLTQTSLDISKIQWNKDIGKS 442

Query: 446 ILESYSRVLESLAFNIIARIDDLLYVDDLTKHSD---RLTSVGTVNVVGHKKAA---YSV 505
           ILESYSR LESLA NIIARIDDLLYVDDLTK SD    L+S    +++ HKK     Y +
Sbjct: 443 ILESYSRALESLASNIIARIDDLLYVDDLTKQSDDNNLLSSPAVSSIIAHKKVVPLPYLI 502

Query: 506 HLSSTPYKLT-ATTPSFSPAPLVSPAKSERMPFLNNRNVGKPIR-----RGFGVKRALTN 565
             S TPY+ + +TTP FSP+ ++SP K ER    ++++  K I      RG+GV+R L N
Sbjct: 503 SASGTPYRTSFSTTPGFSPS-MISPKKGERRTPYSSKDTNKIIEKGLPSRGYGVRRVLNN 562

Query: 566 YLGVETKAKTCLNSIEG--PASIQNIVVNESSERRKSSSADH 589
           YLG+E+K K C+N  +    A I  I  +   E++++S++ H
Sbjct: 563 YLGMESKLKICVNPSDNADTAVINQISKDVEEEKKRNSTSVH 601

BLAST of MS003431 vs. ExPASy Swiss-Prot
Match: Q9LZN0 (Rop guanine nucleotide exchange factor 7 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF7 PE=1 SV=1)

HSP 1 Score: 598.2 bits (1541), Expect = 1.0e-169
Identity = 327/569 (57.47%), Postives = 398/569 (69.95%), Query Frame = 0

Query: 56  GECSSPPQLGWPIRKAQLCKSSNSKVREVEQKPN-------------------SSDSKLS 115
           G   + P++    R++  C S  ++  E EQ P+                     + K  
Sbjct: 3   GSSENLPEVEEKGRESSCCSSETTRQEEEEQSPSCTEDFTASPVSSRWSVKNIDGEKKKI 62

Query: 116 KTAPKVSEMEMMKERFSKLLLGEDMSGSGKGVCTALAISNAITNLCATIFGQLWRLEPLP 175
           ++  +VSE+EMMKERFSKLLLGEDMSGSG GVCTALAISNAITNLCAT+FGQLWRLEPLP
Sbjct: 63  RSDSRVSEVEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCATLFGQLWRLEPLP 122

Query: 176 KEKKSMWRREIEWLLCVSDHIVELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDN 235
            EKK MWRRE+EWLLCVSDHIVE+ P++QTFPDG+KLE+MT RPRSD+++NLPALRKLDN
Sbjct: 123 TEKKEMWRREMEWLLCVSDHIVEMTPTWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDN 182

Query: 236 MLLEILESFVDTEFWYVDQGIVA--SDGDGSSSFRKIVQRQQEKWWLPVPRVPAGGLGED 295
           MLLEIL+SF +TEFWYVDQGI+A  S  DGSSSFRK  QRQ++KWWLPVPRV  GGL E+
Sbjct: 183 MLLEILDSFEETEFWYVDQGIMAHESAADGSSSFRKSFQRQEDKWWLPVPRVSPGGLQEN 242

Query: 296 SRKQLHHTRDCTNQILKAVVAINNIALADMEVPESYLETLPKNGRACLGDVIYRYITSEH 355
           SRKQL H RDCTNQILKA +AIN+I LADME+PESYLE+LP+ GR+CLGD+IYRYI+S+ 
Sbjct: 243 SRKQLQHKRDCTNQILKAAMAINSITLADMEIPESYLESLPRKGRSCLGDLIYRYISSDQ 302

Query: 356 FSSEYLLDCLDLSSEHVALDVANRVEAAIYVWRRRAHSKPQINPTRSTARSSWEMVKDLM 415
           FS E LLDCLDLSSEH A+++ANRVE++IY+W +R +SKP  N      ++SWEMVK+LM
Sbjct: 303 FSPECLLDCLDLSSEHQAIEIANRVESSIYLWHKRTNSKPATN-----TKTSWEMVKELM 362

Query: 416 IDGDKREFLAERAEGLLHSLKQRFPSLTQTTLDTSKIQFNKDVGKSILESYSRVLESLAF 475
           +D DK E +A+RAE LL SLKQRFP L QT LD SKIQ+NKD+GKSILESYSRVLESLAF
Sbjct: 363 VDADKLELMADRAESLLLSLKQRFPGLPQTALDMSKIQYNKDIGKSILESYSRVLESLAF 422

Query: 476 NIIARIDDLLYVDDLTKH-SDRL-TSVGTVNVVGHKKAAYSVHLSSTPYKLTATTPSFSP 535
           NI+ARIDDLL+VDDLT+H SD++ T++G       K  A  V           TTPS+SP
Sbjct: 423 NIVARIDDLLFVDDLTRHSSDQIPTTLGNNGNDAPKSIAVPVS--------NYTTPSYSP 482

Query: 536 ------APLVSPAKSERMPFLNNRNVGKPIRRGFGVKRALTNYLGVETKAKTCLNSIEGP 595
                 + +  P    R            I     VKR LT Y+             +  
Sbjct: 483 SKQELRSSITVPPSPSRF----------KIPHSSSVKRVLTAYV------------TKNE 534

BLAST of MS003431 vs. ExPASy Swiss-Prot
Match: Q9M056 (Rop guanine nucleotide exchange factor 6 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF6 PE=2 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 2.2e-156
Identity = 320/584 (54.79%), Postives = 409/584 (70.03%), Query Frame = 0

Query: 2   EASVKRSENARKIGGSFGSNGVGTESSLTESSAASGESSSSRSSSDTSG-DDVKAGECSS 61
           E S +  E +++I        +G   S+TES+  S  SSSS     +SG   V     SS
Sbjct: 10  EGSKRFGETSKRI--------IGLIDSVTESTTDSSLSSSSSGVGSSSGRSSVAERSVSS 69

Query: 62  PPQ----LGWPIRKAQLCKSSNSKVREVEQKPNSSDSKLSKTAPKVSEMEMMKERFSKLL 121
           PP     LGWP+ +    KSS    ++   K    D    +   + SE+E++KER +KLL
Sbjct: 70  PPTKSQILGWPLGQGSWRKSSGKMKKKTPTK--IDDFGFKRVGTETSEIELLKERMAKLL 129

Query: 122 LGEDMSGSGKGVCTALAISNAITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLCVSDH 181
           LGEDMSGSG+GVC ALAISNAITNL A I GQ WRLEP+P EKK MWRREIE LL VSDH
Sbjct: 130 LGEDMSGSGEGVCPALAISNAITNLYAAILGQQWRLEPIPSEKKLMWRREIEVLLSVSDH 189

Query: 182 IVELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTEFWYVDQG 241
           IVEL+PSFQ FP+G+K+EVM  RPRSD+F  LPALRKLDNML+EIL+SF +TEFWYVDQG
Sbjct: 190 IVELVPSFQNFPNGNKIEVMNCRPRSDLFTCLPALRKLDNMLIEILDSFGETEFWYVDQG 249

Query: 242 IVASDGDGSSSFRKIVQRQQEKWWLPVPRVPAGGLGEDSRKQLHHTRDCTNQILKAVVAI 301
           IVA++   S+SFR+      +KWWLP+PRVP+ GL E +RK+L HTR+ TNQILKA ++I
Sbjct: 250 IVAAESARSNSFRE----DGDKWWLPLPRVPSDGLTEQTRKKLDHTREFTNQILKACMSI 309

Query: 302 NNIALADMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVA 361
           N+IALA+MEVP+SYLE LPKNGR+CLGD +YR ITS++FS+++LL+ +DLSSE   +++A
Sbjct: 310 NSIALAEMEVPQSYLEALPKNGRSCLGDFLYRNITSDNFSADHLLESIDLSSELAVVEMA 369

Query: 362 NRVEAAIYVWRRRAHSKPQINPTRSTARSSWEMVKDLMI---DGDKREFLAERAEGLLHS 421
           NRVEA++YVWRRRAHS+  I+  RST+     +VK++M+   DGDKRE  AERAE LL  
Sbjct: 370 NRVEASMYVWRRRAHSRHLISLYRSTSTRWGMIVKEMMMHQTDGDKREIFAERAESLLIR 429

Query: 422 LKQRFPSLTQTTLDTSKIQFNKDVGKSILESYSRVLESLAFNIIARIDDLLYVDDLTKH- 481
           LKQRFP L QT LDTSKIQ+NKDVGKSILESYSRVLESLA++I  RI+++L++DD++K  
Sbjct: 430 LKQRFPGLRQTALDTSKIQYNKDVGKSILESYSRVLESLAYSIGVRIEEVLFMDDISKDD 489

Query: 482 --------SDRLTSV---------GTVNVVGHKKAAYSVHLS--STPYK---LTATTPSF 541
                   SD+L  +         G++       + +SV  S  STPY+    +A+TPS+
Sbjct: 490 GDGDDDSCSDKLRLLSKEAASGGSGSLREKLSAPSLFSVSFSGTSTPYRTLSFSASTPSY 549

Query: 542 SPAPLVSPAK----SERMPFLNNRNVGKPIRRGFGVKRALTNYL 551
           SP PL+SP       ER PFL+ RN+ +  R GFG K+AL NYL
Sbjct: 550 SPMPLISPINGGRGGERAPFLSGRNIRE--RCGFGPKKALANYL 577

BLAST of MS003431 vs. ExPASy Swiss-Prot
Match: Q93ZY2 (Rop guanine nucleotide exchange factor 1 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF1 PE=1 SV=2)

HSP 1 Score: 489.6 bits (1259), Expect = 5.2e-137
Identity = 251/419 (59.90%), Postives = 317/419 (75.66%), Query Frame = 0

Query: 102 VSEMEMMKERFSKLLLGEDMSGSGKGVCTALAISNAITNLCATIFGQLWRLEPLPKEKKS 161
           +SE+EMMKERF+KLLLGEDMSG GKGVCTALAISNAITNL AT+FG+LWRLEPL  +KK+
Sbjct: 88  LSEIEMMKERFAKLLLGEDMSGGGKGVCTALAISNAITNLSATVFGELWRLEPLAPQKKA 147

Query: 162 MWRREIEWLLCVSDHIVELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEI 221
           MWRRE+EWLLCVSD IVELIPS Q FP G   E+M +RPRSD++ NLPAL+KLD ML+++
Sbjct: 148 MWRRELEWLLCVSDSIVELIPSIQQFPGGGTYEIMETRPRSDLYANLPALKKLDAMLIDM 207

Query: 222 LESFVDTEFWYVDQGIVASDGDGSSSFRKIVQRQQEKWWLPVPRVPAGGLGEDSRKQLHH 281
           L++F DTEFWY D+GIV  D D  S       RQ++KWWLP P+VP  GL E++RK+L  
Sbjct: 208 LDAFSDTEFWYTDRGIVLGDCDKDSYNSPASVRQEDKWWLPCPKVPPNGLSEEARKKLQQ 267

Query: 282 TRDCTNQILKAVVAINNIALADMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLL 341
            RD  NQILKA +AIN+  LA+ME+P+ YLETLPK+G+ CLG++IY+Y+T+  FS E LL
Sbjct: 268 CRDFANQILKAALAINSGVLAEMEIPDPYLETLPKSGKECLGEIIYQYLTANKFSPECLL 327

Query: 342 DCLDLSSEHVALDVANRVEAAIYVWRR---RAHSKPQINPTRSTARSSW-EMVKDLMIDG 401
           DCLDLSSEH  L++ANR+EAA++VWR+   R H K           SSW   VK L+ D 
Sbjct: 328 DCLDLSSEHQTLEIANRIEAAVHVWRQKNGRRHKK-----QAKLKLSSWGGKVKGLVNDN 387

Query: 402 DKREFLAERAEGLLHSLKQRFPSLTQTTLDTSKIQFNKDVGKSILESYSRVLESLAFNII 461
           ++ +FL +RAE LL SL+ RFP L QTTLD +KIQ+NKDVG+SILESYSRV+ES+AFNI 
Sbjct: 388 ERNDFLVQRAETLLQSLRIRFPGLPQTTLDMNKIQYNKDVGQSILESYSRVMESMAFNIT 447

Query: 462 ARIDDLLYVDDLTKHSDRLT---SVGTVNVVGHKKAAYSVHLSSTPYKLTATTPSFSPA 514
           ARIDD+LYVDD  + S  +T   S+ ++N +  +K A+SV   S+P+     TP+ S A
Sbjct: 448 ARIDDVLYVDDAMRRSISVTESLSLFSINGLNPQK-AFSV--QSSPHGSPFATPALSVA 498

BLAST of MS003431 vs. ExPASy Swiss-Prot
Match: A4IJ27 (Rop guanine nucleotide exchange factor 3 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF3 PE=2 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 9.1e-118
Identity = 241/456 (52.85%), Postives = 319/456 (69.96%), Query Frame = 0

Query: 27  SSLTESSAASGESSSSRSSSDTSGDDVKAGECSSPPQLGWPIRKAQLCKSSNSK-VREVE 86
           S+++  S     + S  +S  +   D     CS      WP+    L +S +SK +  +E
Sbjct: 33  STMSIDSFVYPRTCSETTSGFSDQIDETNSFCSEASPCNWPV----LTESKSSKCLSGLE 92

Query: 87  QKPNSSDSKLSKTAPKVSEMEMMKERFSKLLLGEDMSGSGKGVCTALAISNAITNLCATI 146
            + N        + P   E+E MKERF+KLLLGEDMSGSGKGVCTA+ ISNAITNL AT+
Sbjct: 93  MQSNECLVVQEISEP---ELETMKERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATV 152

Query: 147 FGQLWRLEPLPKEKKSMWRREIEWLLCVSDHIVELIPSFQTFPDGSKLEVMTSRPRSDIF 206
           FGQ  RLEPL  EK+++W+RE+  LL V D+IVE IP  Q   +G+ +EVM SRPR+DI+
Sbjct: 153 FGQNLRLEPLETEKRALWKREMNCLLSVCDYIVEFIPRCQNLSNGATVEVMESRPRADIY 212

Query: 207 INLPALRKLDNMLLEILESFVDTEFWYVDQGIVASDGDGSS--SFRK-IVQRQQEKWWLP 266
           INLPALRKLD+ML+E L+SF +TEFWY ++G ++     SS  SFRK IVQR++EKWWLP
Sbjct: 213 INLPALRKLDSMLMEALDSFQNTEFWYAEEGSLSMKSARSSTGSFRKVIVQRKEEKWWLP 272

Query: 267 VPRVPAGGLGEDSRKQLHHTRDCTNQILKAVVAINNIALADMEVPESYLETLPKNGRACL 326
           VP VP+ GL + +RKQL + R+ TNQI KA +AIN+  L++ME+P+SY+ TLPK G++ +
Sbjct: 273 VPLVPSEGLSDKARKQLKNKRESTNQIHKAAMAINSSILSEMEIPDSYMTTLPKCGKSSV 332

Query: 327 GDVIYRYIT-SEHFSSEYLLDCLDLSSEHVALDVANRVEAAIYVWRRRAHSKPQINPTRS 386
           GD IYRY++ S  F  E LLDCL++SSEH A+ +A+RVEA++Y WRR++          S
Sbjct: 333 GDSIYRYMSGSGRFFPEQLLDCLNISSEHEAVQLADRVEASMYTWRRKS--------CLS 392

Query: 387 TARSSWEMVKDLMID---GDKREFLAERAEGLLHSLKQRFPSLTQTTLDTSKIQFNKDVG 446
            +++SW MVKDLM      DK   +AERAE LL  LKQR+P L+QT+LD  KIQ+NKDVG
Sbjct: 393 NSKNSWNMVKDLMSTTERTDKNYVMAERAETLLFCLKQRYPELSQTSLDICKIQYNKDVG 452

Query: 447 KSILESYSRVLESLAFNIIARIDDLLYVDDLTKHSD 475
           K++LESYSRVLE LAFNI+A IDD+LYVD   + S+
Sbjct: 453 KAVLESYSRVLEGLAFNIVAWIDDVLYVDKTMRGSE 473

BLAST of MS003431 vs. ExPASy TrEMBL
Match: A0A6J1C216 (rop guanine nucleotide exchange factor 5 OS=Momordica charantia OX=3673 GN=LOC111006698 PE=4 SV=1)

HSP 1 Score: 1156.0 bits (2989), Expect = 0.0e+00
Identity = 596/602 (99.00%), Postives = 598/602 (99.34%), Query Frame = 0

Query: 1   MEASVKRSENARKIGGSFGSNGVGTESSLTESSAASGESSSSRSSSDTSGDDVKAGECSS 60
           MEASVKRSENA KI GSFGSNGVGTESSLTESSAASGESSSSRSSSDTSGDDVKAGECSS
Sbjct: 1   MEASVKRSENAPKISGSFGSNGVGTESSLTESSAASGESSSSRSSSDTSGDDVKAGECSS 60

Query: 61  PPQLGWPIRKAQLCKSSNSKVREVEQKPNSSDSKLSKTAPKVSEMEMMKERFSKLLLGED 120
           PPQLGWPIRKAQLCKSSNSKVREVEQKPNSSDSKLSKTAPKVSEMEMMKERFSKLLLGED
Sbjct: 61  PPQLGWPIRKAQLCKSSNSKVREVEQKPNSSDSKLSKTAPKVSEMEMMKERFSKLLLGED 120

Query: 121 MSGSGKGVCTALAISNAITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLCVSDHIVEL 180
           MSGSGKGVCTALAISNAITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLCVSDHIVEL
Sbjct: 121 MSGSGKGVCTALAISNAITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLCVSDHIVEL 180

Query: 181 IPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTEFWYVDQGIVAS 240
           IPSFQTFPDGSKLEVMTSR RSDIFINLPALRKLDNMLLEILESFVDTEFWYVDQGIVAS
Sbjct: 181 IPSFQTFPDGSKLEVMTSRSRSDIFINLPALRKLDNMLLEILESFVDTEFWYVDQGIVAS 240

Query: 241 DGDGSSSFRKIVQRQQEKWWLPVPRVPAGGLGEDSRKQLHHTRDCTNQILKAVVAINNIA 300
           DGDGSSSFRKIVQRQQEKWWLPVPRVPAGGLGEDSRKQLHHTRDCTNQILKAVVAINNIA
Sbjct: 241 DGDGSSSFRKIVQRQQEKWWLPVPRVPAGGLGEDSRKQLHHTRDCTNQILKAVVAINNIA 300

Query: 301 LADMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVANRVE 360
           LADMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVANRVE
Sbjct: 301 LADMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVANRVE 360

Query: 361 AAIYVWRRRAHSKPQINPTRSTARSSWEMVKDLMIDGDKREFLAERAEGLLHSLKQRFPS 420
           AAIYVWRRRAHSKPQINPTRSTARSSWEMVKDLMIDGDKREFLAERAEGLLHSLKQRFPS
Sbjct: 361 AAIYVWRRRAHSKPQINPTRSTARSSWEMVKDLMIDGDKREFLAERAEGLLHSLKQRFPS 420

Query: 421 LTQTTLDTSKIQFNKDVGKSILESYSRVLESLAFNIIARIDDLLYVDDLTKHSDRLTSVG 480
           LTQTTLDTSKIQFNKDVGKSILESYSRVLESLAFNIIARIDDLLYVDDLTKHSDRLTSVG
Sbjct: 421 LTQTTLDTSKIQFNKDVGKSILESYSRVLESLAFNIIARIDDLLYVDDLTKHSDRLTSVG 480

Query: 481 TVNVVGHKKAAYSVHLSSTPYKLTATTPSFSPAPLVSPAKSERMPFLNNRNVGKPIRRGF 540
           TVNVVGHKKAAYSVHLSSTPYKLTATTPSFSPAPLVSPAKSERMPFLNNRNVGKPIRRGF
Sbjct: 481 TVNVVGHKKAAYSVHLSSTPYKLTATTPSFSPAPLVSPAKSERMPFLNNRNVGKPIRRGF 540

Query: 541 GVKRALTNYLGVETKAKTCLNSIEGPASIQNIVVNESSERRKSSSADHSVVSCSKLLADK 600
           GVKRALTNYLGVETKAKTCLNSIEGPASIQNIVVNESSERRKSSSAD SVVSCSKLL+DK
Sbjct: 541 GVKRALTNYLGVETKAKTCLNSIEGPASIQNIVVNESSERRKSSSADQSVVSCSKLLSDK 600

Query: 601 VK 603
           +K
Sbjct: 601 IK 602

BLAST of MS003431 vs. ExPASy TrEMBL
Match: A0A0A0K7N2 (PRONE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G008860 PE=4 SV=1)

HSP 1 Score: 1057.7 bits (2734), Expect = 1.8e-305
Identity = 546/602 (90.70%), Postives = 570/602 (94.68%), Query Frame = 0

Query: 1   MEASVKRSENARKIGGSFGSNGVGTESSLTESSAASGESSSSRSSSDTSGDDVKAGECSS 60
           MEASV  ++NARKI  SFGS G GTE   TESSA SG+SSSSRSSSD S     AGECSS
Sbjct: 1   MEASVNTTDNARKIATSFGSIGAGTE-LFTESSAVSGQSSSSRSSSDNS-----AGECSS 60

Query: 61  PPQLGWPIRKAQLCKSSNSKVREVEQKPNSSDSKLSKTAPKVSEMEMMKERFSKLLLGED 120
           PPQLGWPIRKAQLCKSSNS+VREVEQKP+S+DSK SKT  KVSEME+MKERF+KLLLGED
Sbjct: 61  PPQLGWPIRKAQLCKSSNSRVREVEQKPSSTDSKPSKTVTKVSEMELMKERFAKLLLGED 120

Query: 121 MSGSGKGVCTALAISNAITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLCVSDHIVEL 180
           MSGSGKGV TALAISN+ITNLCATIFGQLWRLEPLPKEKKSMW+RE+EWLLCVSDHIVEL
Sbjct: 121 MSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKKSMWKRELEWLLCVSDHIVEL 180

Query: 181 IPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTEFWYVDQGIVAS 240
           IPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESF DTEFWYVDQGIV+S
Sbjct: 181 IPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFADTEFWYVDQGIVSS 240

Query: 241 DGDGSSSFRKIVQRQQEKWWLPVPRVPAGGLGEDSRKQLHHTRDCTNQILKAVVAINNIA 300
           DGDGSSSFRKIVQRQQEKWWLPVPRVPAGGLGEDSRKQLHHTRDCTNQILKAV+AINNIA
Sbjct: 241 DGDGSSSFRKIVQRQQEKWWLPVPRVPAGGLGEDSRKQLHHTRDCTNQILKAVMAINNIA 300

Query: 301 LADMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVANRVE 360
           L DMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVANRVE
Sbjct: 301 LNDMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVANRVE 360

Query: 361 AAIYVWRRRAHSKPQINPTRSTARSSWEMVKDLMIDGDKREFLAERAEGLLHSLKQRFPS 420
           AAIYVWRRRAHSKPQINPTRSTARSSWEMVKDLMIDGDKREFLAERAEGLLHSLKQRFPS
Sbjct: 361 AAIYVWRRRAHSKPQINPTRSTARSSWEMVKDLMIDGDKREFLAERAEGLLHSLKQRFPS 420

Query: 421 LTQTTLDTSKIQFNKDVGKSILESYSRVLESLAFNIIARIDDLLYVDDLTKHSDRLTSVG 480
           LTQTTLDTSKIQFNKDVGKSILESYSRVLESLAFNIIARIDDLLYVDDLTKHS+RLTS  
Sbjct: 421 LTQTTLDTSKIQFNKDVGKSILESYSRVLESLAFNIIARIDDLLYVDDLTKHSNRLTSAA 480

Query: 481 TVNVVGHKKAAYSVHLSSTPYKLTATTPSFSPAPLVSPAKSERMPFLNNRNVGKPIRRGF 540
           TVNVVGHKKAAYSVHLSSTPYKLT+TTPSFSPAPLVSPAK+ER+PFLNN+   KP RRGF
Sbjct: 481 TVNVVGHKKAAYSVHLSSTPYKLTSTTPSFSPAPLVSPAKTERVPFLNNKITSKPPRRGF 540

Query: 541 GVKRALTNYLGVETKAKTCLNSIEGPASIQNIVVNESSERRKSSSADHSVVSCSKLLADK 600
           GVKRALTNYLGVETK KTC NSIEG ASIQNI+VNE+SE+RKSSSAD SV+SCSKLLA+K
Sbjct: 541 GVKRALTNYLGVETKPKTCTNSIEGAASIQNIIVNENSEQRKSSSADQSVISCSKLLAEK 596

Query: 601 VK 603
           +K
Sbjct: 601 MK 596

BLAST of MS003431 vs. ExPASy TrEMBL
Match: A0A5D3DL37 (Rop guanine nucleotide exchange factor 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold205G001260 PE=4 SV=1)

HSP 1 Score: 1055.0 bits (2727), Expect = 1.1e-304
Identity = 542/602 (90.03%), Postives = 570/602 (94.68%), Query Frame = 0

Query: 1   MEASVKRSENARKIGGSFGSNGVGTESSLTESSAASGESSSSRSSSDTSGDDVKAGECSS 60
           MEASVK ++NARKI  SFGS G GTE   TESSA SG+SSSSRSSSD S     AGECSS
Sbjct: 1   MEASVKTTDNARKIAASFGSIGAGTE-LFTESSAVSGQSSSSRSSSDNS-----AGECSS 60

Query: 61  PPQLGWPIRKAQLCKSSNSKVREVEQKPNSSDSKLSKTAPKVSEMEMMKERFSKLLLGED 120
           PPQLGWPIRKAQLCKSSNS+VREVEQKP+S+DSK SKT  KVSEME+MKERF+KLLLGED
Sbjct: 61  PPQLGWPIRKAQLCKSSNSRVREVEQKPSSNDSKPSKTVTKVSEMELMKERFAKLLLGED 120

Query: 121 MSGSGKGVCTALAISNAITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLCVSDHIVEL 180
           MSGSGKGV TALAISN+ITNLCAT+FGQLWRLEPLPKEKKSMW+RE+EWLLCVSDHIVEL
Sbjct: 121 MSGSGKGVSTALAISNSITNLCATVFGQLWRLEPLPKEKKSMWKRELEWLLCVSDHIVEL 180

Query: 181 IPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTEFWYVDQGIVAS 240
           IPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESF DTEFWYVDQGIV+S
Sbjct: 181 IPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFADTEFWYVDQGIVSS 240

Query: 241 DGDGSSSFRKIVQRQQEKWWLPVPRVPAGGLGEDSRKQLHHTRDCTNQILKAVVAINNIA 300
           DGDGSSSFRKIVQRQQEKWWLPVPRVPAGGL EDSRKQLHHTRDCTNQILKAV+AINNIA
Sbjct: 241 DGDGSSSFRKIVQRQQEKWWLPVPRVPAGGLAEDSRKQLHHTRDCTNQILKAVMAINNIA 300

Query: 301 LADMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVANRVE 360
           L DMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVANRVE
Sbjct: 301 LNDMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVANRVE 360

Query: 361 AAIYVWRRRAHSKPQINPTRSTARSSWEMVKDLMIDGDKREFLAERAEGLLHSLKQRFPS 420
           AAIYVWRRRAHSKPQINPTRSTARSSWEMVKDLMIDGDKREFLAERAEGLLHSLKQRFPS
Sbjct: 361 AAIYVWRRRAHSKPQINPTRSTARSSWEMVKDLMIDGDKREFLAERAEGLLHSLKQRFPS 420

Query: 421 LTQTTLDTSKIQFNKDVGKSILESYSRVLESLAFNIIARIDDLLYVDDLTKHSDRLTSVG 480
           LTQTTLDTSKIQFNKD+GKSILESYSRVLESLA+NIIARIDDLLYVDDLTKHS+RLTS  
Sbjct: 421 LTQTTLDTSKIQFNKDIGKSILESYSRVLESLAYNIIARIDDLLYVDDLTKHSNRLTSAA 480

Query: 481 TVNVVGHKKAAYSVHLSSTPYKLTATTPSFSPAPLVSPAKSERMPFLNNRNVGKPIRRGF 540
           TVNVVGHKKAAYSVHLSSTPYKLT+TTPSFSPAPLVSPAK+ER+PFLNN+   KP RRGF
Sbjct: 481 TVNVVGHKKAAYSVHLSSTPYKLTSTTPSFSPAPLVSPAKTERVPFLNNKITSKPPRRGF 540

Query: 541 GVKRALTNYLGVETKAKTCLNSIEGPASIQNIVVNESSERRKSSSADHSVVSCSKLLADK 600
           GVKRALTNYLGVETK +TC NSIEG ASIQNI+VNE+SE+RKSSSAD SV+SCSKLLA+K
Sbjct: 541 GVKRALTNYLGVETKPRTCTNSIEGAASIQNIIVNENSEQRKSSSADQSVISCSKLLAEK 596

Query: 601 VK 603
           +K
Sbjct: 601 MK 596

BLAST of MS003431 vs. ExPASy TrEMBL
Match: A0A1S3B2X5 (rop guanine nucleotide exchange factor 5 OS=Cucumis melo OX=3656 GN=LOC103485538 PE=4 SV=1)

HSP 1 Score: 1055.0 bits (2727), Expect = 1.1e-304
Identity = 542/602 (90.03%), Postives = 570/602 (94.68%), Query Frame = 0

Query: 1   MEASVKRSENARKIGGSFGSNGVGTESSLTESSAASGESSSSRSSSDTSGDDVKAGECSS 60
           MEASVK ++NARKI  SFGS G GTE   TESSA SG+SSSSRSSSD S     AGECSS
Sbjct: 1   MEASVKTTDNARKIAASFGSIGAGTE-LFTESSAVSGQSSSSRSSSDNS-----AGECSS 60

Query: 61  PPQLGWPIRKAQLCKSSNSKVREVEQKPNSSDSKLSKTAPKVSEMEMMKERFSKLLLGED 120
           PPQLGWPIRKAQLCKSSNS+VREVEQKP+S+DSK SKT  KVSEME+MKERF+KLLLGED
Sbjct: 61  PPQLGWPIRKAQLCKSSNSRVREVEQKPSSNDSKPSKTVTKVSEMELMKERFAKLLLGED 120

Query: 121 MSGSGKGVCTALAISNAITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLCVSDHIVEL 180
           MSGSGKGV TALAISN+ITNLCAT+FGQLWRLEPLPKEKKSMW+RE+EWLLCVSDHIVEL
Sbjct: 121 MSGSGKGVSTALAISNSITNLCATVFGQLWRLEPLPKEKKSMWKRELEWLLCVSDHIVEL 180

Query: 181 IPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTEFWYVDQGIVAS 240
           IPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESF DTEFWYVDQGIV+S
Sbjct: 181 IPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFADTEFWYVDQGIVSS 240

Query: 241 DGDGSSSFRKIVQRQQEKWWLPVPRVPAGGLGEDSRKQLHHTRDCTNQILKAVVAINNIA 300
           DGDGSSSFRKIVQRQQEKWWLPVPRVPAGGL EDSRKQLHHTRDCTNQILKAV+AINNIA
Sbjct: 241 DGDGSSSFRKIVQRQQEKWWLPVPRVPAGGLAEDSRKQLHHTRDCTNQILKAVMAINNIA 300

Query: 301 LADMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVANRVE 360
           L DMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVANRVE
Sbjct: 301 LNDMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVANRVE 360

Query: 361 AAIYVWRRRAHSKPQINPTRSTARSSWEMVKDLMIDGDKREFLAERAEGLLHSLKQRFPS 420
           AAIYVWRRRAHSKPQINPTRSTARSSWEMVKDLMIDGDKREFLAERAEGLLHSLKQRFPS
Sbjct: 361 AAIYVWRRRAHSKPQINPTRSTARSSWEMVKDLMIDGDKREFLAERAEGLLHSLKQRFPS 420

Query: 421 LTQTTLDTSKIQFNKDVGKSILESYSRVLESLAFNIIARIDDLLYVDDLTKHSDRLTSVG 480
           LTQTTLDTSKIQFNKD+GKSILESYSRVLESLA+NIIARIDDLLYVDDLTKHS+RLTS  
Sbjct: 421 LTQTTLDTSKIQFNKDIGKSILESYSRVLESLAYNIIARIDDLLYVDDLTKHSNRLTSAA 480

Query: 481 TVNVVGHKKAAYSVHLSSTPYKLTATTPSFSPAPLVSPAKSERMPFLNNRNVGKPIRRGF 540
           TVNVVGHKKAAYSVHLSSTPYKLT+TTPSFSPAPLVSPAK+ER+PFLNN+   KP RRGF
Sbjct: 481 TVNVVGHKKAAYSVHLSSTPYKLTSTTPSFSPAPLVSPAKTERVPFLNNKITSKPPRRGF 540

Query: 541 GVKRALTNYLGVETKAKTCLNSIEGPASIQNIVVNESSERRKSSSADHSVVSCSKLLADK 600
           GVKRALTNYLGVETK +TC NSIEG ASIQNI+VNE+SE+RKSSSAD SV+SCSKLLA+K
Sbjct: 541 GVKRALTNYLGVETKPRTCTNSIEGAASIQNIIVNENSEQRKSSSADQSVISCSKLLAEK 596

Query: 601 VK 603
           +K
Sbjct: 601 MK 596

BLAST of MS003431 vs. ExPASy TrEMBL
Match: A0A6J1JUB1 (rop guanine nucleotide exchange factor 5-like OS=Cucurbita maxima OX=3661 GN=LOC111489797 PE=4 SV=1)

HSP 1 Score: 1020.4 bits (2637), Expect = 3.1e-294
Identity = 529/602 (87.87%), Postives = 555/602 (92.19%), Query Frame = 0

Query: 1   MEASVKRSENARKIGGSFGSNGVGTESSLTESSAASGESSSSRSSSDTSGDDVKAGECSS 60
           ME SVKRSE ARK    FGSNG GTE S TE SA SGESSSSRSSSD+S DD+KA ECSS
Sbjct: 1   MEDSVKRSEKARK----FGSNGAGTE-SFTEFSAVSGESSSSRSSSDSSADDLKAVECSS 60

Query: 61  PPQLGWPIRKAQLCKSSNSKVREVEQKPNSSDSKLSKTAPKVSEMEMMKERFSKLLLGED 120
           PPQLGWPIRKAQ  K+SNS+    EQ  NS+ SK++KT PKVSE+EMMKERF+KLLLGED
Sbjct: 61  PPQLGWPIRKAQPSKTSNSR----EQNSNSNGSKINKTVPKVSELEMMKERFAKLLLGED 120

Query: 121 MSGSGKGVCTALAISNAITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLCVSDHIVEL 180
           MSGSGKGV TALAISN+ITNLCATIFGQLWRLEPLP EKKSMWRRE+EWLLCVS HIVEL
Sbjct: 121 MSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPTEKKSMWRREMEWLLCVSGHIVEL 180

Query: 181 IPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTEFWYVDQGIVAS 240
           IPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESF DTEFWYVDQGIV++
Sbjct: 181 IPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFTDTEFWYVDQGIVSA 240

Query: 241 DGDGSSSFRKIVQRQQEKWWLPVPRVPAGGLGEDSRKQLHHTRDCTNQILKAVVAINNIA 300
           DGDGSSSFRKIVQRQQEKWWLPVPRVP GGLGEDSRKQLHH RDCT+QILKAV AINNIA
Sbjct: 241 DGDGSSSFRKIVQRQQEKWWLPVPRVPVGGLGEDSRKQLHHIRDCTSQILKAVKAINNIA 300

Query: 301 LADMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVANRVE 360
           L DMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSS+HVALDVANRVE
Sbjct: 301 LNDMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSDHVALDVANRVE 360

Query: 361 AAIYVWRRRAHSKPQINPTRSTARSSWEMVKDLMIDGDKREFLAERAEGLLHSLKQRFPS 420
           AAIYVWRRRAHSKPQ+NPTRS+ RSSWEMVKDLMIDGDKREFLAERAEG LHSLKQRFP+
Sbjct: 361 AAIYVWRRRAHSKPQLNPTRSSTRSSWEMVKDLMIDGDKREFLAERAEGFLHSLKQRFPT 420

Query: 421 LTQTTLDTSKIQFNKDVGKSILESYSRVLESLAFNIIARIDDLLYVDDLTKHSDRLTSVG 480
           LTQTTLDTSKIQFNKDVGKSILESYSRVLES+A+NIIARIDDLLYVDDLTKHS RLTSV 
Sbjct: 421 LTQTTLDTSKIQFNKDVGKSILESYSRVLESIAYNIIARIDDLLYVDDLTKHSGRLTSVA 480

Query: 481 TVNVVGHKKAAYSVHLSSTPYKLTATTPSFSPAPLVSPAKSERMPFLNNRNVGKPIRRGF 540
           TVNVVGHKKAAYSVH SSTPYKLT+TTPSFSPAP VSPAK+ER PFLNN N  KP RRGF
Sbjct: 481 TVNVVGHKKAAYSVHFSSTPYKLTSTTPSFSPAPKVSPAKTERPPFLNNNNNSKPPRRGF 540

Query: 541 GVKRALTNYLGVETKAKTCLNSIEGPASIQNIVVNESSERRKSSSADHSVVSCSKLLADK 600
           GVKRALTNYL VETKAKTC NSIEGPASIQNIVVNESSE+RKSSSAD +V++CSKLLAD 
Sbjct: 541 GVKRALTNYLSVETKAKTCTNSIEGPASIQNIVVNESSEQRKSSSADQNVINCSKLLADT 593

Query: 601 VK 603
            K
Sbjct: 601 TK 593

BLAST of MS003431 vs. TAIR 10
Match: AT5G05940.1 (ROP guanine nucleotide exchange factor 5 )

HSP 1 Score: 650.6 bits (1677), Expect = 1.2e-186
Identity = 354/582 (60.82%), Postives = 430/582 (73.88%), Query Frame = 0

Query: 26  ESSLTESSAASGESSSSRSSSDTSGDDVKAGECSSPPQ--LGWPIRKAQLCKSSNSKVRE 85
           E  LTES   S  S +S  SS T+   V +     PP   LGWPIRKA   K+S   V  
Sbjct: 23  EDILTESKERSSTSGASYESSSTT--TVASSSPPPPPSQILGWPIRKASFRKNSKESVNF 82

Query: 86  VEQKPN-SSDSKLSKTAPKVSEMEMMKERFSKLLLGEDMSGSGKGVCTALAISNAITNLC 145
             +K     DS         +++EMMKERF+KLLLGEDMSGSGKGVCTALAISNAITNLC
Sbjct: 83  DHKKLTLHDDSGFKAKEMNSADVEMMKERFAKLLLGEDMSGSGKGVCTALAISNAITNLC 142

Query: 146 ATIFGQLWRLEPLPKEKKSMWRREIEWLLCVSDHIVELIPSFQTFPDGSKLEVMTSRPRS 205
           ATIFGQLWRLEPL  EKK MWRRE+EW+L VSDHIVEL PS QT+PDG+K EVMT RPR 
Sbjct: 143 ATIFGQLWRLEPLSSEKKEMWRREMEWILSVSDHIVELTPSTQTYPDGNKFEVMTCRPRF 202

Query: 206 DIFINLPALRKLDNMLLEILESFVDTEFWYVDQGIVASDGDGSSSFRKIVQRQQEKWWLP 265
           D+FINLPALRKLDNMLL+IL SF  TEFWYVDQGIVAS+ DGS+SFR+ +QRQ+EKWWLP
Sbjct: 203 DLFINLPALRKLDNMLLDILASFKKTEFWYVDQGIVASENDGSASFRRKIQRQEEKWWLP 262

Query: 266 VPRVPAGGLGEDSRKQLHHTRDCTNQILKAVVAINNIALADMEVPESYLETLPKNGRACL 325
           VPR+   GL E++R +L+H R+C  QILKA +AIN++AL +M+VP+SYLETLPKNGR+CL
Sbjct: 263 VPRLAPNGLTEEARTELNHKRECATQILKAAMAINSLALTEMDVPKSYLETLPKNGRSCL 322

Query: 326 GDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVANRVEAAIYVWRRRAHSKPQINPTRS- 385
           GDVIYRY+TS+ FS+E LLDCLDLSSEH+ALD+ANRVEA+IYVWRRR  +K  +N   S 
Sbjct: 323 GDVIYRYVTSDKFSAESLLDCLDLSSEHIALDIANRVEASIYVWRRRVQTKLGVNNNTSS 382

Query: 386 -TARSSWEMVKDLMIDGDKREFLAERAEGLLHSLKQRFPSLTQTTLDTSKIQFNKDVGKS 445
            T + +WEMVK+LM  GDKR  L ER+E LL  LKQRFPSLTQT+LD SKIQ+NKD+GKS
Sbjct: 383 TTPKLTWEMVKELMAAGDKRGLLVERSETLLRCLKQRFPSLTQTSLDISKIQWNKDIGKS 442

Query: 446 ILESYSRVLESLAFNIIARIDDLLYVDDLTKHSD---RLTSVGTVNVVGHKKAA---YSV 505
           ILESYSR LESLA NIIARIDDLLYVDDLTK SD    L+S    +++ HKK     Y +
Sbjct: 443 ILESYSRALESLASNIIARIDDLLYVDDLTKQSDDNNLLSSPAVSSIIAHKKVVPLPYLI 502

Query: 506 HLSSTPYKLT-ATTPSFSPAPLVSPAKSERMPFLNNRNVGKPIR-----RGFGVKRALTN 565
             S TPY+ + +TTP FSP+ ++SP K ER    ++++  K I      RG+GV+R L N
Sbjct: 503 SASGTPYRTSFSTTPGFSPS-MISPKKGERRTPYSSKDTNKIIEKGLPSRGYGVRRVLNN 562

Query: 566 YLGVETKAKTCLNSIEG--PASIQNIVVNESSERRKSSSADH 589
           YLG+E+K K C+N  +    A I  I  +   E++++S++ H
Sbjct: 563 YLGMESKLKICVNPSDNADTAVINQISKDVEEEKKRNSTSVH 601

BLAST of MS003431 vs. TAIR 10
Match: AT5G02010.1 (RHO guanyl-nucleotide exchange factor 7 )

HSP 1 Score: 598.2 bits (1541), Expect = 7.3e-171
Identity = 327/569 (57.47%), Postives = 398/569 (69.95%), Query Frame = 0

Query: 56  GECSSPPQLGWPIRKAQLCKSSNSKVREVEQKPN-------------------SSDSKLS 115
           G   + P++    R++  C S  ++  E EQ P+                     + K  
Sbjct: 3   GSSENLPEVEEKGRESSCCSSETTRQEEEEQSPSCTEDFTASPVSSRWSVKNIDGEKKKI 62

Query: 116 KTAPKVSEMEMMKERFSKLLLGEDMSGSGKGVCTALAISNAITNLCATIFGQLWRLEPLP 175
           ++  +VSE+EMMKERFSKLLLGEDMSGSG GVCTALAISNAITNLCAT+FGQLWRLEPLP
Sbjct: 63  RSDSRVSEVEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCATLFGQLWRLEPLP 122

Query: 176 KEKKSMWRREIEWLLCVSDHIVELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDN 235
            EKK MWRRE+EWLLCVSDHIVE+ P++QTFPDG+KLE+MT RPRSD+++NLPALRKLDN
Sbjct: 123 TEKKEMWRREMEWLLCVSDHIVEMTPTWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDN 182

Query: 236 MLLEILESFVDTEFWYVDQGIVA--SDGDGSSSFRKIVQRQQEKWWLPVPRVPAGGLGED 295
           MLLEIL+SF +TEFWYVDQGI+A  S  DGSSSFRK  QRQ++KWWLPVPRV  GGL E+
Sbjct: 183 MLLEILDSFEETEFWYVDQGIMAHESAADGSSSFRKSFQRQEDKWWLPVPRVSPGGLQEN 242

Query: 296 SRKQLHHTRDCTNQILKAVVAINNIALADMEVPESYLETLPKNGRACLGDVIYRYITSEH 355
           SRKQL H RDCTNQILKA +AIN+I LADME+PESYLE+LP+ GR+CLGD+IYRYI+S+ 
Sbjct: 243 SRKQLQHKRDCTNQILKAAMAINSITLADMEIPESYLESLPRKGRSCLGDLIYRYISSDQ 302

Query: 356 FSSEYLLDCLDLSSEHVALDVANRVEAAIYVWRRRAHSKPQINPTRSTARSSWEMVKDLM 415
           FS E LLDCLDLSSEH A+++ANRVE++IY+W +R +SKP  N      ++SWEMVK+LM
Sbjct: 303 FSPECLLDCLDLSSEHQAIEIANRVESSIYLWHKRTNSKPATN-----TKTSWEMVKELM 362

Query: 416 IDGDKREFLAERAEGLLHSLKQRFPSLTQTTLDTSKIQFNKDVGKSILESYSRVLESLAF 475
           +D DK E +A+RAE LL SLKQRFP L QT LD SKIQ+NKD+GKSILESYSRVLESLAF
Sbjct: 363 VDADKLELMADRAESLLLSLKQRFPGLPQTALDMSKIQYNKDIGKSILESYSRVLESLAF 422

Query: 476 NIIARIDDLLYVDDLTKH-SDRL-TSVGTVNVVGHKKAAYSVHLSSTPYKLTATTPSFSP 535
           NI+ARIDDLL+VDDLT+H SD++ T++G       K  A  V           TTPS+SP
Sbjct: 423 NIVARIDDLLFVDDLTRHSSDQIPTTLGNNGNDAPKSIAVPVS--------NYTTPSYSP 482

Query: 536 ------APLVSPAKSERMPFLNNRNVGKPIRRGFGVKRALTNYLGVETKAKTCLNSIEGP 595
                 + +  P    R            I     VKR LT Y+             +  
Sbjct: 483 SKQELRSSITVPPSPSRF----------KIPHSSSVKRVLTAYV------------TKNE 534

BLAST of MS003431 vs. TAIR 10
Match: AT3G55660.1 (ROP (rho of plants) guanine nucleotide exchange factor 6 )

HSP 1 Score: 553.9 bits (1426), Expect = 1.6e-157
Identity = 320/584 (54.79%), Postives = 409/584 (70.03%), Query Frame = 0

Query: 2   EASVKRSENARKIGGSFGSNGVGTESSLTESSAASGESSSSRSSSDTSG-DDVKAGECSS 61
           E S +  E +++I        +G   S+TES+  S  SSSS     +SG   V     SS
Sbjct: 10  EGSKRFGETSKRI--------IGLIDSVTESTTDSSLSSSSSGVGSSSGRSSVAERSVSS 69

Query: 62  PPQ----LGWPIRKAQLCKSSNSKVREVEQKPNSSDSKLSKTAPKVSEMEMMKERFSKLL 121
           PP     LGWP+ +    KSS    ++   K    D    +   + SE+E++KER +KLL
Sbjct: 70  PPTKSQILGWPLGQGSWRKSSGKMKKKTPTK--IDDFGFKRVGTETSEIELLKERMAKLL 129

Query: 122 LGEDMSGSGKGVCTALAISNAITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLCVSDH 181
           LGEDMSGSG+GVC ALAISNAITNL A I GQ WRLEP+P EKK MWRREIE LL VSDH
Sbjct: 130 LGEDMSGSGEGVCPALAISNAITNLYAAILGQQWRLEPIPSEKKLMWRREIEVLLSVSDH 189

Query: 182 IVELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTEFWYVDQG 241
           IVEL+PSFQ FP+G+K+EVM  RPRSD+F  LPALRKLDNML+EIL+SF +TEFWYVDQG
Sbjct: 190 IVELVPSFQNFPNGNKIEVMNCRPRSDLFTCLPALRKLDNMLIEILDSFGETEFWYVDQG 249

Query: 242 IVASDGDGSSSFRKIVQRQQEKWWLPVPRVPAGGLGEDSRKQLHHTRDCTNQILKAVVAI 301
           IVA++   S+SFR+      +KWWLP+PRVP+ GL E +RK+L HTR+ TNQILKA ++I
Sbjct: 250 IVAAESARSNSFRE----DGDKWWLPLPRVPSDGLTEQTRKKLDHTREFTNQILKACMSI 309

Query: 302 NNIALADMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVA 361
           N+IALA+MEVP+SYLE LPKNGR+CLGD +YR ITS++FS+++LL+ +DLSSE   +++A
Sbjct: 310 NSIALAEMEVPQSYLEALPKNGRSCLGDFLYRNITSDNFSADHLLESIDLSSELAVVEMA 369

Query: 362 NRVEAAIYVWRRRAHSKPQINPTRSTARSSWEMVKDLMI---DGDKREFLAERAEGLLHS 421
           NRVEA++YVWRRRAHS+  I+  RST+     +VK++M+   DGDKRE  AERAE LL  
Sbjct: 370 NRVEASMYVWRRRAHSRHLISLYRSTSTRWGMIVKEMMMHQTDGDKREIFAERAESLLIR 429

Query: 422 LKQRFPSLTQTTLDTSKIQFNKDVGKSILESYSRVLESLAFNIIARIDDLLYVDDLTKH- 481
           LKQRFP L QT LDTSKIQ+NKDVGKSILESYSRVLESLA++I  RI+++L++DD++K  
Sbjct: 430 LKQRFPGLRQTALDTSKIQYNKDVGKSILESYSRVLESLAYSIGVRIEEVLFMDDISKDD 489

Query: 482 --------SDRLTSV---------GTVNVVGHKKAAYSVHLS--STPYK---LTATTPSF 541
                   SD+L  +         G++       + +SV  S  STPY+    +A+TPS+
Sbjct: 490 GDGDDDSCSDKLRLLSKEAASGGSGSLREKLSAPSLFSVSFSGTSTPYRTLSFSASTPSY 549

Query: 542 SPAPLVSPAK----SERMPFLNNRNVGKPIRRGFGVKRALTNYL 551
           SP PL+SP       ER PFL+ RN+ +  R GFG K+AL NYL
Sbjct: 550 SPMPLISPINGGRGGERAPFLSGRNIRE--RCGFGPKKALANYL 577

BLAST of MS003431 vs. TAIR 10
Match: AT4G38430.1 (rho guanyl-nucleotide exchange factor 1 )

HSP 1 Score: 489.6 bits (1259), Expect = 3.7e-138
Identity = 251/419 (59.90%), Postives = 317/419 (75.66%), Query Frame = 0

Query: 102 VSEMEMMKERFSKLLLGEDMSGSGKGVCTALAISNAITNLCATIFGQLWRLEPLPKEKKS 161
           +SE+EMMKERF+KLLLGEDMSG GKGVCTALAISNAITNL AT+FG+LWRLEPL  +KK+
Sbjct: 88  LSEIEMMKERFAKLLLGEDMSGGGKGVCTALAISNAITNLSATVFGELWRLEPLAPQKKA 147

Query: 162 MWRREIEWLLCVSDHIVELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEI 221
           MWRRE+EWLLCVSD IVELIPS Q FP G   E+M +RPRSD++ NLPAL+KLD ML+++
Sbjct: 148 MWRRELEWLLCVSDSIVELIPSIQQFPGGGTYEIMETRPRSDLYANLPALKKLDAMLIDM 207

Query: 222 LESFVDTEFWYVDQGIVASDGDGSSSFRKIVQRQQEKWWLPVPRVPAGGLGEDSRKQLHH 281
           L++F DTEFWY D+GIV  D D  S       RQ++KWWLP P+VP  GL E++RK+L  
Sbjct: 208 LDAFSDTEFWYTDRGIVLGDCDKDSYNSPASVRQEDKWWLPCPKVPPNGLSEEARKKLQQ 267

Query: 282 TRDCTNQILKAVVAINNIALADMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLL 341
            RD  NQILKA +AIN+  LA+ME+P+ YLETLPK+G+ CLG++IY+Y+T+  FS E LL
Sbjct: 268 CRDFANQILKAALAINSGVLAEMEIPDPYLETLPKSGKECLGEIIYQYLTANKFSPECLL 327

Query: 342 DCLDLSSEHVALDVANRVEAAIYVWRR---RAHSKPQINPTRSTARSSW-EMVKDLMIDG 401
           DCLDLSSEH  L++ANR+EAA++VWR+   R H K           SSW   VK L+ D 
Sbjct: 328 DCLDLSSEHQTLEIANRIEAAVHVWRQKNGRRHKK-----QAKLKLSSWGGKVKGLVNDN 387

Query: 402 DKREFLAERAEGLLHSLKQRFPSLTQTTLDTSKIQFNKDVGKSILESYSRVLESLAFNII 461
           ++ +FL +RAE LL SL+ RFP L QTTLD +KIQ+NKDVG+SILESYSRV+ES+AFNI 
Sbjct: 388 ERNDFLVQRAETLLQSLRIRFPGLPQTTLDMNKIQYNKDVGQSILESYSRVMESMAFNIT 447

Query: 462 ARIDDLLYVDDLTKHSDRLT---SVGTVNVVGHKKAAYSVHLSSTPYKLTATTPSFSPA 514
           ARIDD+LYVDD  + S  +T   S+ ++N +  +K A+SV   S+P+     TP+ S A
Sbjct: 448 ARIDDVLYVDDAMRRSISVTESLSLFSINGLNPQK-AFSV--QSSPHGSPFATPALSVA 498

BLAST of MS003431 vs. TAIR 10
Match: AT4G00460.2 (RHO guanyl-nucleotide exchange factor 3 )

HSP 1 Score: 425.6 bits (1093), Expect = 6.5e-119
Identity = 241/456 (52.85%), Postives = 319/456 (69.96%), Query Frame = 0

Query: 27  SSLTESSAASGESSSSRSSSDTSGDDVKAGECSSPPQLGWPIRKAQLCKSSNSK-VREVE 86
           S+++  S     + S  +S  +   D     CS      WP+    L +S +SK +  +E
Sbjct: 33  STMSIDSFVYPRTCSETTSGFSDQIDETNSFCSEASPCNWPV----LTESKSSKCLSGLE 92

Query: 87  QKPNSSDSKLSKTAPKVSEMEMMKERFSKLLLGEDMSGSGKGVCTALAISNAITNLCATI 146
            + N        + P   E+E MKERF+KLLLGEDMSGSGKGVCTA+ ISNAITNL AT+
Sbjct: 93  MQSNECLVVQEISEP---ELETMKERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATV 152

Query: 147 FGQLWRLEPLPKEKKSMWRREIEWLLCVSDHIVELIPSFQTFPDGSKLEVMTSRPRSDIF 206
           FGQ  RLEPL  EK+++W+RE+  LL V D+IVE IP  Q   +G+ +EVM SRPR+DI+
Sbjct: 153 FGQNLRLEPLETEKRALWKREMNCLLSVCDYIVEFIPRCQNLSNGATVEVMESRPRADIY 212

Query: 207 INLPALRKLDNMLLEILESFVDTEFWYVDQGIVASDGDGSS--SFRK-IVQRQQEKWWLP 266
           INLPALRKLD+ML+E L+SF +TEFWY ++G ++     SS  SFRK IVQR++EKWWLP
Sbjct: 213 INLPALRKLDSMLMEALDSFQNTEFWYAEEGSLSMKSARSSTGSFRKVIVQRKEEKWWLP 272

Query: 267 VPRVPAGGLGEDSRKQLHHTRDCTNQILKAVVAINNIALADMEVPESYLETLPKNGRACL 326
           VP VP+ GL + +RKQL + R+ TNQI KA +AIN+  L++ME+P+SY+ TLPK G++ +
Sbjct: 273 VPLVPSEGLSDKARKQLKNKRESTNQIHKAAMAINSSILSEMEIPDSYMTTLPKCGKSSV 332

Query: 327 GDVIYRYIT-SEHFSSEYLLDCLDLSSEHVALDVANRVEAAIYVWRRRAHSKPQINPTRS 386
           GD IYRY++ S  F  E LLDCL++SSEH A+ +A+RVEA++Y WRR++          S
Sbjct: 333 GDSIYRYMSGSGRFFPEQLLDCLNISSEHEAVQLADRVEASMYTWRRKS--------CLS 392

Query: 387 TARSSWEMVKDLMID---GDKREFLAERAEGLLHSLKQRFPSLTQTTLDTSKIQFNKDVG 446
            +++SW MVKDLM      DK   +AERAE LL  LKQR+P L+QT+LD  KIQ+NKDVG
Sbjct: 393 NSKNSWNMVKDLMSTTERTDKNYVMAERAETLLFCLKQRYPELSQTSLDICKIQYNKDVG 452

Query: 447 KSILESYSRVLESLAFNIIARIDDLLYVDDLTKHSD 475
           K++LESYSRVLE LAFNI+A IDD+LYVD   + S+
Sbjct: 453 KAVLESYSRVLEGLAFNIVAWIDDVLYVDKTMRGSE 473