MS003164 (gene) Bitter gourd (TR) v1

Overview
NameMS003164
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionNuclear/nucleolar GTPase 2
Locationscaffold234: 828113 .. 834323 (-)
RNA-Seq ExpressionMS003164
SyntenyMS003164
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGAAGAAGAAGGAAAAGAAGGTAAATGTCTCTGGGAAACCGAAGCATTCACTGGACGTCAACCGAAGCGATGCGAACAAGAATTCGCGTAGCGCTGCCACTGTGCGGCGCCTCAAGATGTATAATACGAGGCCGAAGCGTGATCGGAAAGGGAAGGTGTTGAAGAATGAGCTTCAGTCGAAGGAGTTACCTGACACGCGGATTCAGCCTGATCGCCGCTGGTTCGGTATGTTGCATTATATCAAATGGAGTTCCACGTGCATGCGTCATTTATTTTCTTGCTTTTATGTTTGAATTGCTGGGTTCTTCTCGGTATCTTTCTCATCCCTGCGTGATAAAAGGGTGGATTAGTCTGAATTAGTACTTGTTTTTACAGTTTTATTTTTTGTGGGTTTGTTAACGGTGAGGGAGCATGATATATATTGTTCTTTGTTGAGGTTGTAGGAAGAAGAGTGAACGTCAGCTTGGAGCCTAAGGTTTCCGTTTTAGCTGATTAGGAGAAGCCTAGAGTAATAAGAATTGTCAAATCATGAATGCCTACCTCTCTCCTTTTTCGTTATTAAACAACTTGTATTCTTAGAACTATCATGCTGTATGTTGACAAAATTTTCTGAGAGGAAGCATAATGATGTTTATTTCTTGAATTGATAGGGAATACTCGAGTTGTGAACCAGAAAGAGCTTGAATTTTTCCGCGAAGAGCTTCAAAAACGGATGTCATGTAGCTATAATGTGATTTTGAAGGAAAAGAAGCTCCCCCTGTCCTTGTTGAATGATCAACAGAAGGTAGAAGATAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACATTTCATTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAAAGTTTTTACATTAATTTTCAAGGTGAGGTGCTCATGTATTAGTGTGTTTACCTCTGCTGCCAATGTTCTCAGCTCTTGTACTGCATATGTTTTTCAAGAGTAAGATTATCTTTTAGATGAAGAAAAGTTGTCCACTGAATATGTTTAGGTTGTTATCTCTCACTTTTCCGATCTGGCCTTACTCATTTATTCATATAGATGTAGCTTTGAAGGTTCTATGACGTGCTTGCTTATGTTAAGATAATAATCCAATGTGATACCTTAGTTAAAAGGGAATTCTTTTGTAAACTAAGGAAGTAGAAAATGTCCAACTTCGATCTGCTAATATGGTACTTTGATCTCTGAAGCAATCTAGAGTCCATCTTCTCGATACAGAACCTTTTGAGGATGCATTTGGGCCAAAGGGGAAGAGAAAGCGACCAAAGCTTTTGGCTGCTGACTATGAGTCACTACTTAAGAAAGCTGATAAGTCTCATGGTATGATATATTTGATTCAGTCTCTTGAATTTGCAGATTTGGGTAAACACATGTTTTTTTCTAGGATTTTAAAGAATAGTTGTCAATAACTGTGGTGTTCTTTTTTATAAGGCATATATAATGCTGCTGCCTGCTCTCAATAAATGTTTTTCATTTCTCATGGAATTTTTGTAGTTGTCACCCACATATGTCAAGAGTGCTGTTCTCAATATTGCATATTCTGTATTCTTTAGATGCCTTTGAGGAAAAGCATGCTGATAATGCAACTGCAGAGGGAAGTGAAGGTGATGGATTTAGAGACCTAGTTCGACATAATATGTTTGAGAAGGGTCAAAGTAAACGTATATGGGGTGAGCTCTACAAAGTAATTGATTCTTCAGATGTTGTTGTCCAGGTTCGTCGTAGTTATTAATCTCTTCCCCAATTTGACGACAGCATTCACATGGGACATTTCCTATTCTGATTTATATATTTTTTTTCCCAATAGTTTATGGCATGTAAAAGCTAGTATCTACCTAAAGGTTATTTACCTTATTTCTAGTGGTTTTATTATTATTATTATTATTATTATTATTATAAAAAAAGGTATCTATTTACGTTAGTTCTAGATTTTTAGAAAAAAGGTGTCTTATAGTAGTTTGCACAGCCTTGTATTTTATTTCATGCTTCTCAATGAAAGGTTGTTTCTCATTAAAAAAAAGTTTTCACAGCCACACATTATGAATGTGGCTTTCTATTATCATTTATGTGTTAATTCTTTTTATTGTTTTCGATGTCAAACTTGTGGTTTGTTTTCCCTCAACTAAATGTGGGGCGCCCTAATTCCTTGTATATCCGTTTGACTTCTCTTCTGATTATTTGTCATTGTTCCAGGTTCTAGATGCAAGGGACCCTCAAGGAACAAGATGTTACCATTTAGAGAGACATTTGAAAGAGCATTGCAAGCATAAACACGTGGTTCTTCTGTTAAATAAGGTTTCACCACATCCTTTTTAATTGGATTTTAATGTTATTTGAGATACCTAGTTATGGACTTTTTGAAAATTTTTGTAAGGCTGATTGAGACATGTTAACAAATGGCATTGTCAGTGTGATTTGATTCCTGCTTGGGCAACAAAGGGATGGCTTAGAGTGTTATCAAAAGAATATCCAACTCTAGCATTTCATGCAAGCGTCAACAAATCCTTTGGAAAGGTAACTCTACCATTATTATGTGACTCAATAAGTTATTTACGTGTATTGTTTTTAATCAAAAAGGATTTTAACTCCTAAAATGCTGTTACTCATAAATATTGCCAATTTTTTAATATTTCATTTTTAGTTGAGAGCCAGACATGATAGATGTATGTCGTCCCTAACCAAATTTGAGGCTTCAGGTGCCTTTTTCTAAAACCTCTTTCATCCCATATCCTGTGTTGGAAGGTGAAAGGTTTATAATTTATACTCCTATGAGATTGTTTCTTATAATTTGAGTTTTACATTCCTAGAGTGTACGTTTTTCTTCCGATACTGGAGACCAGGATATTTTAGATGTGTTCTGACCATATTGGTATAATATTCGGTCTTTTACTCCCATAAAATAACATTGTGTACCATCTGAAGCTTTGTGTCTTATGCTCCTTGTTGGTCAATGCTATCTTGATGTTTAGACGATATTGGTTTATCAGGAAAATCAAAAAAAAAAAAAGGGGGATCATTTGATATATCACACTTTAGGAATTATAGGATAAGCATCATCTTTGACTTCATTTCTAAGACCCTTTTTTGTTGTTCTTAGGGTTCTCTCCTATCTGTGCTGAGACAATTTGCTCGCTTGAAAAGTGACAAGCAAGCTATCTCCGTGGGATTTGTTGGGTATCCCAATGTTGGAAAGTCATCTGTAATTAACACATTACGGACTAAGAATGTAAGTTTCTATTTTTCTCTAATGCTGACAATGTTATTATATTGATACCAAAAATTTATCGTTTAATTTGTTCTACATTCATTTGGTGGTATGCCTGAATTATGAATGGAGAAACTGCAGTTGTTATTTAGCATCCAAAAGGGTCTATTGTTAGCTTTCAGAACAATCAAGGCAAATTTTGGATGCCTCAAATCATAATATGCTAACATTTATGAGTTGTTTAGTCTGATCCAGATTACTTCCTGTTTTTGTCTGGATCTCTGTTAGGTTAGCATGTTTTCTGGGGAACTTTATTCTTCATATGAATGCTTTTGAGCGTATGGCTTGCTTTGTAGTGTACAATCTTTTGATAATTTTCACTCAGTGGATCACCAGAGTGATGGACCACCAATTAGATTCACATATGTACGTACAATAAGAGAAATTCTTAATATATGAGCACAGAGTTGGAAGGAGAATTTCTAGAAATCCGAAGGGGGTGGAGGTAACCAGTCTGATACCCAGTTTTTTGTTAAGCCTAGTTCAGGTAGCAGCCAATCGAGCTAAATACAAGGCTTAGCTAAATATAAATGTAAATGCTAGAGGAGTTAGTCACGTGAATATTAGATTGGAGACTGAGGTGGCAGAGTCTAAAATTTTGCTTCTTTGACTTTTCTTTCTTATCAATGAAACTACCGAGGGGAACATTCTCCTGAATTGAGATTTCTTAACCTATCAAAGAGCTTGTGTGCAAAATAGTGTATTTTGATTGCAGTGTCTTACCTTTTGCTTTTAGATGGTAATTAGAGCTTTCAGCTGCTTGAAGCTGTTCCATCTTTTGGCCATCTGCCTTCCATAATAGTTTTGTCATTGTTGCTGTGACAAAGAAGGAAATTCAGGATGTTAAAATTCCCAAACCTAAAGTCACTCAGTTTTTATTCTTATATTGTTCTTGATTTATAGAAGTGTAAAAAAAGATATATTTTAAGGTTTTCTAAAGACGGGAAGCTTTTGATGTGAAAGATGAAAAGTTTATGTACAAGTATATCCCCACCCTGATGTTAAAAGAACAATAAAGAGCTCCAATCTGGTGGAAATAGGAAAAGAATTGGGACCTGTTAATTAAAATTTGAACTTCTTGTCCTCTGTTGCTTTGCTTGTAGTAGTAACACTTAGGTGAATCCCTTACGGTCCATTCTTCTGGCACTTATAATCAATCCCCTACAGTTTGTTCTCTTTTTTATCTGACTTTGCCCTTTTTCTTATTAGGTATGCAAAGTCGCACCAATTCCTGGGGAAACTAAAGTGTGGCAATATATAACTCTCACAAAGAGGATTTTCCTGATTGACTGCCCGGGTGTTGTTTATCAAAATAGTGACTCTGAAACAGATATTGTTCTTAAGGGCGTGGTATATGTTCTCTCTTTTTCATTTTCTTTGCTAACTGGTGTCTATTGTTACCATATATGAGCATGAGCTTTCAATTAATGGATATTGCAATTACCATTTTGCCAGCTTGTTATTATTATTTTTTTCATTCAATATTATTGTCTGAGTATTTGGTATTTGGCCACTTCTCTAGGTACGAGTTACAAATTTGGAGGATGCATCTGAACATATTGGAGAAGTTTTGAAGCGTGTGAAGAGGGAACACCTTGNATATTGTCGCCATGCAACGCATTTATAATGTCATGGGGGGTTATTATTATTATTAGGAACCTAAAACTTAAGGACTTAAATTGATTTGTTGAAAGTTTTAAGGACTATAATAAAAGTAAACTGAGAAGTTGAGGACCAATATGAGATTTATATTTCGCCTGATAAAAAACAGTAGGTCGTTGAAAAGGTGAAAGACCCTCTTTTAATGGTTGGACCCATGAAAATCTCCTAACTGTTCTTTTACCTGCTCATTCAACTACTCTGTTCTAGTGTGCTGCAACTGTTAGCATAGTGCTGGTATTAGACGACAAAGAATGAAGCTTGCATGAATTGTTCTTTTTGAAAAATTATCTCTCTCAGTAAAATTATTTCTAATTCACTAGTTTGCTTATTATACGTATTGCTGCTGACTTTCTAATATTTCTTTCTAAACTTTGTTGTCTCGTGTTTAGGGAGGACGATAATGACTTCTTAATTCAGCTATGCAAGTTGACTGGCAAACTGTTAAGGGTACGTTATTATCTTGAGAGTGGTTCCTTATTATCTGTATGGCATCCATTTAAAATACTGATCAAAGGATTTTATGTTTTCATTGTGTATAAGATGATAAAGGTTTGCAGTCTGTATTTAAAGAAAACAGGATTGTTTTGTCCTTTCCCCTTATCCTTGATACATGATAATGTTGTGTTAGTACTCGAAATGGTTGGGTATCCTAATTGATTAGGCAATCTGCCTCATAAATTTATCATCAGTCCTCAAAATAGGAATTGATGGCTCGGACTAATAATAATCTGGTTTCTTGTGATTTGAAGAAAACTAACTGTTTCAAGTGGGATGGTCATCTATCCAGGGCGGCGAACCTGACTTGACAACTGCAGCAAAAATGGTCCTCCATGACTGGCAGAGGGGTAGACTTCCCTTCTTTGTACCGCCACCTCGAGTAGATGATAAGTCGGAAGAACCCGACTATGGTGTGGATGATGACTCAGGCGTGGATAGCAATCAGGCTGCAGCTGCTTTCAAAGCCATTGCAAATGTGATATCATCTCAGCAACAAAGAAATGTGCCTGTTCAAAGGGATCTGTTTAGTGATACAGAATTGAATGGCGATGCATCTGATCAGATTCTAGTCTCCGAGGATGAGCTAGAGGCTCCTCTTTCTGACACCGAGGATAAGACATCTGGAGACCAGGATGACGTGGTTGAAGATCAGCGTCCAACCGCAGGC

mRNA sequence

ATGACGAAGAAGAAGGAAAAGAAGGTAAATGTCTCTGGGAAACCGAAGCATTCACTGGACGTCAACCGAAGCGATGCGAACAAGAATTCGCGTAGCGCTGCCACTGTGCGGCGCCTCAAGATGTATAATACGAGGCCGAAGCGTGATCGGAAAGGGAAGGTGTTGAAGAATGAGCTTCAGTCGAAGGAGTTACCTGACACGCGGATTCAGCCTGATCGCCGCTGGTTCGGGAATACTCGAGTTGTGAACCAGAAAGAGCTTGAATTTTTCCGCGAAGAGCTTCAAAAACGGATGTCATGTAGCTATAATGTGATTTTGAAGGAAAAGAAGCTCCCCCTGTCCTTGTTGAATGATCAACAGAAGCAATCTAGAGTCCATCTTCTCGATACAGAACCTTTTGAGGATGCATTTGGGCCAAAGGGGAAGAGAAAGCGACCAAAGCTTTTGGCTGCTGACTATGAGTCACTACTTAAGAAAGCTGATAAGTCTCATGATGCCTTTGAGGAAAAGCATGCTGATAATGCAACTGCAGAGGGAAGTGAAGGTGATGGATTTAGAGACCTAGTTCGACATAATATGTTTGAGAAGGGTCAAAGTAAACGTATATGGGGTGAGCTCTACAAAGTAATTGATTCTTCAGATGTTGTTGTCCAGGTTCTAGATGCAAGGGACCCTCAAGGAACAAGATGTTACCATTTAGAGAGACATTTGAAAGAGCATTGCAAGCATAAACACGTGGTTCTTCTGTTAAATAAGTGTGATTTGATTCCTGCTTGGGCAACAAAGGGATGGCTTAGAGTGTTATCAAAAGAATATCCAACTCTAGCATTTCATGCAAGCGTCAACAAATCCTTTGGAAAGGGTTCTCTCCTATCTGTGCTGAGACAATTTGCTCGCTTGAAAAGTGACAAGCAAGCTATCTCCGTGGGATTTGTTGGGTATCCCAATGTTGGAAAGTCATCTGTAATTAACACATTACGGACTAAGAATGTATGCAAAGTCGCACCAATTCCTGGGGAAACTAAAGTGTGGCAATATATAACTCTCACAAAGAGGATTTTCCTGATTGACTGCCCGGGTGTTGTTTATCAAAATAGTGACTCTGAAACAGATATTGTTCTTAAGGGCGTGGTACGAGTTACAAATTTGGAGGATGCATCTGAACATATTGGAGAAGTTTTGAAGCGTGTGAAGAGGGAACACCTTGNATATTGTCGCCATGCAACGATAATGTCATGGGGGGACGATAATGACTTCTTAATTCAGCTATGCAAGTTGACTGGCAAACTGTTAAGGGGCGGCGAACCTGACTTGACAACTGCAGCAAAAATGGTCCTCCATGACTGGCAGAGGGGTAGACTTCCCTTCTTTGTACCGCCACCTCGAGTAGATGATAAGTCGGAAGAACCCGACTATGGTGTGGATGATGACTCAGGCGTGGATAGCAATCAGGCTGCAGCTGCTTTCAAAGCCATTGCAAATGTGATATCATCTCAGCAACAAAGAAATGTGCCTGTTCAAAGGGATCTGTTTAGTGATACAGAATTGAATGGCGATGCATCTGATCAGATTCTAGTCTCCGAGGATGAGCTAGAGGCTCCTCTTTCTGACACCGAGGATAAGACATCTGGAGACCAGGATGACGTGGTTGAAGATCAGCGTCCAACCGCAGGC

Coding sequence (CDS)

ATGACGAAGAAGAAGGAAAAGAAGGTAAATGTCTCTGGGAAACCGAAGCATTCACTGGACGTCAACCGAAGCGATGCGAACAAGAATTCGCGTAGCGCTGCCACTGTGCGGCGCCTCAAGATGTATAATACGAGGCCGAAGCGTGATCGGAAAGGGAAGGTGTTGAAGAATGAGCTTCAGTCGAAGGAGTTACCTGACACGCGGATTCAGCCTGATCGCCGCTGGTTCGGGAATACTCGAGTTGTGAACCAGAAAGAGCTTGAATTTTTCCGCGAAGAGCTTCAAAAACGGATGTCATGTAGCTATAATGTGATTTTGAAGGAAAAGAAGCTCCCCCTGTCCTTGTTGAATGATCAACAGAAGCAATCTAGAGTCCATCTTCTCGATACAGAACCTTTTGAGGATGCATTTGGGCCAAAGGGGAAGAGAAAGCGACCAAAGCTTTTGGCTGCTGACTATGAGTCACTACTTAAGAAAGCTGATAAGTCTCATGATGCCTTTGAGGAAAAGCATGCTGATAATGCAACTGCAGAGGGAAGTGAAGGTGATGGATTTAGAGACCTAGTTCGACATAATATGTTTGAGAAGGGTCAAAGTAAACGTATATGGGGTGAGCTCTACAAAGTAATTGATTCTTCAGATGTTGTTGTCCAGGTTCTAGATGCAAGGGACCCTCAAGGAACAAGATGTTACCATTTAGAGAGACATTTGAAAGAGCATTGCAAGCATAAACACGTGGTTCTTCTGTTAAATAAGTGTGATTTGATTCCTGCTTGGGCAACAAAGGGATGGCTTAGAGTGTTATCAAAAGAATATCCAACTCTAGCATTTCATGCAAGCGTCAACAAATCCTTTGGAAAGGGTTCTCTCCTATCTGTGCTGAGACAATTTGCTCGCTTGAAAAGTGACAAGCAAGCTATCTCCGTGGGATTTGTTGGGTATCCCAATGTTGGAAAGTCATCTGTAATTAACACATTACGGACTAAGAATGTATGCAAAGTCGCACCAATTCCTGGGGAAACTAAAGTGTGGCAATATATAACTCTCACAAAGAGGATTTTCCTGATTGACTGCCCGGGTGTTGTTTATCAAAATAGTGACTCTGAAACAGATATTGTTCTTAAGGGCGTGGTACGAGTTACAAATTTGGAGGATGCATCTGAACATATTGGAGAAGTTTTGAAGCGTGTGAAGAGGGAACACCTTGNATATTGTCGCCATGCAACGATAATGTCATGGGGGGACGATAATGACTTCTTAATTCAGCTATGCAAGTTGACTGGCAAACTGTTAAGGGGCGGCGAACCTGACTTGACAACTGCAGCAAAAATGGTCCTCCATGACTGGCAGAGGGGTAGACTTCCCTTCTTTGTACCGCCACCTCGAGTAGATGATAAGTCGGAAGAACCCGACTATGGTGTGGATGATGACTCAGGCGTGGATAGCAATCAGGCTGCAGCTGCTTTCAAAGCCATTGCAAATGTGATATCATCTCAGCAACAAAGAAATGTGCCTGTTCAAAGGGATCTGTTTAGTGATACAGAATTGAATGGCGATGCATCTGATCAGATTCTAGTCTCCGAGGATGAGCTAGAGGCTCCTCTTTCTGACACCGAGGATAAGACATCTGGAGACCAGGATGACGTGGTTGAAGATCAGCGTCCAACCGCAGGC

Protein sequence

MTKKKEKKVNVSGKPKHSLDVNRSDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNELQSKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDQQKQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEEKHADNATAEGSEGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEHCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARLKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPGVVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLXYCRHATIMSWGDDNDFLIQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSGVDSNQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTEDKTSGDQDDVVEDQRPTAG
Homology
BLAST of MS003164 vs. NCBI nr
Match: XP_022133818.1 (nuclear/nucleolar GTPase 2 isoform X1 [Momordica charantia])

HSP 1 Score: 1075.1 bits (2779), Expect = 0.0e+00
Identity = 547/558 (98.03%), Postives = 547/558 (98.03%), Query Frame = 0

Query: 1   MTKKKEKKVNVSGKPKHSLDVNRSDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ 60
           MTKKKEKKVNVSGKPKHSLDVNRSDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ
Sbjct: 1   MTKKKEKKVNVSGKPKHSLDVNRSDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ 60

Query: 61  SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDQQ 120
           SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLND Q
Sbjct: 61  SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDHQ 120

Query: 121 KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEEKHADNATAEGS 180
           KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEE HADNATAEGS
Sbjct: 121 KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEENHADNATAEGS 180

Query: 181 EGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH 240
           EGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH
Sbjct: 181 EGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH 240

Query: 241 CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARL 300
           CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARL
Sbjct: 241 CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARL 300

Query: 301 KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG 360
           KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG
Sbjct: 301 KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG 360

Query: 361 VVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLXYCRHATIMSWGDDNDFL 420
           VVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHL   R   I  W DDNDFL
Sbjct: 361 VVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLE--RAYKIKDWEDDNDFL 420

Query: 421 IQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSGV 480
           IQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSGV
Sbjct: 421 IQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSGV 480

Query: 481 DSNQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTED 540
           DSNQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTED
Sbjct: 481 DSNQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTED 540

Query: 541 KTSGDQDDVVEDQRPTAG 559
           KTSGDQDDVVEDQRPTAG
Sbjct: 541 KTSGDQDDVVEDQRPTAG 556

BLAST of MS003164 vs. NCBI nr
Match: XP_022133820.1 (nuclear/nucleolar GTPase 2 isoform X2 [Momordica charantia])

HSP 1 Score: 1042.7 bits (2695), Expect = 1.1e-300
Identity = 535/558 (95.88%), Postives = 535/558 (95.88%), Query Frame = 0

Query: 1   MTKKKEKKVNVSGKPKHSLDVNRSDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ 60
           MTKKKEKKVNVSGKPKHSLDVNRSDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ
Sbjct: 1   MTKKKEKKVNVSGKPKHSLDVNRSDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ 60

Query: 61  SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDQQ 120
           SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLND Q
Sbjct: 61  SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDHQ 120

Query: 121 KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEEKHADNATAEGS 180
           KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSH             EGS
Sbjct: 121 KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSH-------------EGS 180

Query: 181 EGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH 240
           EGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH
Sbjct: 181 EGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH 240

Query: 241 CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARL 300
           CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARL
Sbjct: 241 CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARL 300

Query: 301 KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG 360
           KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG
Sbjct: 301 KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG 360

Query: 361 VVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLXYCRHATIMSWGDDNDFL 420
           VVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHL   R   I  W DDNDFL
Sbjct: 361 VVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLE--RAYKIKDWEDDNDFL 420

Query: 421 IQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSGV 480
           IQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSGV
Sbjct: 421 IQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSGV 480

Query: 481 DSNQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTED 540
           DSNQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTED
Sbjct: 481 DSNQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTED 540

Query: 541 KTSGDQDDVVEDQRPTAG 559
           KTSGDQDDVVEDQRPTAG
Sbjct: 541 KTSGDQDDVVEDQRPTAG 543

BLAST of MS003164 vs. NCBI nr
Match: XP_038890331.1 (nuclear/nucleolar GTPase 2 [Benincasa hispida] >XP_038890332.1 nuclear/nucleolar GTPase 2 [Benincasa hispida])

HSP 1 Score: 1003.4 bits (2593), Expect = 7.5e-289
Identity = 508/558 (91.04%), Postives = 530/558 (94.98%), Query Frame = 0

Query: 1   MTKKKEKKVNVSGKPKHSLDVNRSDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ 60
           M KKKEKKVNVSGKPKHSLDVNRS+ NKN RSAATVRRLKMYNTRPKRDRKGKVLKNELQ
Sbjct: 1   MAKKKEKKVNVSGKPKHSLDVNRSNENKNGRSAATVRRLKMYNTRPKRDRKGKVLKNELQ 60

Query: 61  SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDQQ 120
           SKELPDTRIQPDRRWFGNTRVVNQKELE FREEL+KRMS SYNVILKE+KLPLSLLND Q
Sbjct: 61  SKELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSLLNDHQ 120

Query: 121 KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEEKHADNATAEGS 180
           KQSRVHLLDTEPF+DAFGPKGKRKRPKLLAADYESLL+KADKSHD FEEK+A+NAT EGS
Sbjct: 121 KQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAENATIEGS 180

Query: 181 EGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH 240
           E DGFRDLVRH MFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH
Sbjct: 181 EEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH 240

Query: 241 CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARL 300
           CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHAS+NKSFGKGSLLSVLRQFARL
Sbjct: 241 CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFARL 300

Query: 301 KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG 360
           KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG
Sbjct: 301 KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG 360

Query: 361 VVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLXYCRHATIMSWGDDNDFL 420
           VVYQNSD+ETDIVLKGVVRVTNLEDASEHIGEVLKRVK+EHL   R   I  WGDDNDFL
Sbjct: 361 VVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLE--RAYKIKEWGDDNDFL 420

Query: 421 IQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSGV 480
           +QLCKLTGKLLRGGEPDLTTAAKMVLHDWQRG+LPFFV PPRV+D+SEEP+Y VD+DSGV
Sbjct: 421 VQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGKLPFFVSPPRVEDESEEPNYCVDEDSGV 480

Query: 481 DSNQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTED 540
           DSNQAAAAFKAIANVISSQQQR+VPVQRDLFSD ELNG+ SD ILVSE+EL+AP SDTE 
Sbjct: 481 DSNQAAAAFKAIANVISSQQQRSVPVQRDLFSDNELNGETSDHILVSENELQAPPSDTEG 540

Query: 541 KTSGDQDDVVEDQRPTAG 559
           KTSGD+DD  ED+RP AG
Sbjct: 541 KTSGDEDDDNEDERPIAG 556

BLAST of MS003164 vs. NCBI nr
Match: TYK01950.1 (nuclear/nucleolar GTPase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 999.2 bits (2582), Expect = 1.4e-287
Identity = 507/558 (90.86%), Postives = 529/558 (94.80%), Query Frame = 0

Query: 1   MTKKKEKKVNVSGKPKHSLDVNRS-DANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNEL 60
           M KKKEKKVNVSGKPKHSLDVNRS + NKN RSAATVRRLKMYNTRPKRDRKGKVLKN+L
Sbjct: 1   MAKKKEKKVNVSGKPKHSLDVNRSNEKNKNGRSAATVRRLKMYNTRPKRDRKGKVLKNDL 60

Query: 61  QSKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDQ 120
           QS ELPDTRIQPDRRWFGNTRVVNQKELE FREEL+KRMS SYNVILKE+KLPLSLLND 
Sbjct: 61  QSNELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSLLNDH 120

Query: 121 QKQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEEKHADNATAEG 180
           QKQSRVHLLDTEPF+DAFGPKGKRKRPKLLAADYESLL+KADKSHD FEEK+A+NAT EG
Sbjct: 121 QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAENATVEG 180

Query: 181 SEGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 240
           SE DGFRDLVRH MFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE
Sbjct: 181 SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 240

Query: 241 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFAR 300
           HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHAS+NKSFGKGSLLSVLRQFAR
Sbjct: 241 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR 300

Query: 301 LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP 360
           LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP
Sbjct: 301 LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP 360

Query: 361 GVVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLXYCRHATIMSWGDDNDF 420
           GVVYQNSD+ETDIVLKGVVRVTNLEDASEHIGEVLKRVK+EHL   R   I +W DDNDF
Sbjct: 361 GVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLE--RAYKIKNWEDDNDF 420

Query: 421 LIQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSG 480
           L+QLCKLTGKLLRGGEPDLTTAAKMVLHDWQRG+LPFFVPPPRV+D+SEEP+YGVDDDSG
Sbjct: 421 LVQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGKLPFFVPPPRVEDESEEPNYGVDDDSG 480

Query: 481 VDSNQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTE 540
           VDSNQAAAAFKAIANVISSQQQR+VPVQRDLFS+ ELNG+ SDQ LVSEDEL+APLSDTE
Sbjct: 481 VDSNQAAAAFKAIANVISSQQQRSVPVQRDLFSENELNGETSDQTLVSEDELQAPLSDTE 540

Query: 541 DKTSGDQDDVVEDQRPTA 558
            KTSGDQDD  ED+   A
Sbjct: 541 GKTSGDQDDKNEDEHTIA 556

BLAST of MS003164 vs. NCBI nr
Match: XP_022993572.1 (nuclear/nucleolar GTPase 2 [Cucurbita maxima] >XP_022993573.1 nuclear/nucleolar GTPase 2 [Cucurbita maxima] >XP_022993574.1 nuclear/nucleolar GTPase 2 [Cucurbita maxima])

HSP 1 Score: 992.6 bits (2565), Expect = 1.3e-285
Identity = 508/560 (90.71%), Postives = 531/560 (94.82%), Query Frame = 0

Query: 1   MTKKKEKKVNVSGKPKHSLDVNRSDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ 60
           MTKK E+KV+VSGKPKHSLD NRSD NKN+RSAATVRRLKMYNTRPKRDRKGKVLK+ELQ
Sbjct: 1   MTKKTERKVSVSGKPKHSLDANRSDGNKNARSAATVRRLKMYNTRPKRDRKGKVLKHELQ 60

Query: 61  SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDQQ 120
           SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMS SYNVILKEKKLPLSLLND Q
Sbjct: 61  SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSSSYNVILKEKKLPLSLLNDHQ 120

Query: 121 KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEEKHADNATAEGS 180
           KQSRVHLLDTEPF+DAFGPKGKRKRPKLLAADYESLLKKADKSHD F+EK+A+NAT EGS
Sbjct: 121 KQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLKKADKSHDDFDEKYAENATNEGS 180

Query: 181 EGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH 240
           E DGFRDLVRH MFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH
Sbjct: 181 EEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH 240

Query: 241 CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARL 300
           CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHAS+NKSFGKGSLLSVLRQFARL
Sbjct: 241 CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFARL 300

Query: 301 KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG 360
           KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG
Sbjct: 301 KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG 360

Query: 361 VVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLXYCRHATIMSWGDDNDFL 420
           VVYQNSD+ETD+VLKGVVRVTNLED SEHIGEVLKRVK+EHL   R   I +W DDNDFL
Sbjct: 361 VVYQNSDTETDVVLKGVVRVTNLEDPSEHIGEVLKRVKKEHLE--RAYKIKNWVDDNDFL 420

Query: 421 IQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKS-EEPDYGVDDDSG 480
           +QLCKL+GKLLRGGEPDLTT AKMVLHDWQRG+LPFFVPPPRV+DKS EEP+Y VDDDSG
Sbjct: 421 VQLCKLSGKLLRGGEPDLTTVAKMVLHDWQRGKLPFFVPPPRVEDKSEEEPNYSVDDDSG 480

Query: 481 VDSNQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDA-SDQILVSEDELEAPLSDT 540
           VDSNQAAAAFKAIANVISSQQQR+VPVQRDLFSD ELNG++  DQILVSEDEL+AP SDT
Sbjct: 481 VDSNQAAAAFKAIANVISSQQQRSVPVQRDLFSDNELNGESCDDQILVSEDELQAP-SDT 540

Query: 541 EDKTSGDQDDVVEDQRPTAG 559
           E +TS  QDDVVED RPTAG
Sbjct: 541 EGRTSEGQDDVVEDVRPTAG 557

BLAST of MS003164 vs. ExPASy Swiss-Prot
Match: Q9C923 (Nuclear/nucleolar GTPase 2 OS=Arabidopsis thaliana OX=3702 GN=NUG2 PE=1 SV=1)

HSP 1 Score: 799.3 bits (2063), Expect = 2.8e-230
Identity = 406/552 (73.55%), Postives = 469/552 (84.96%), Query Frame = 0

Query: 4   KKEKKVNVSGKPKHSLDVNRSDANK---NSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ 63
           KKEKK NVSGKPKHSLD NR+D  K    +RS +TV RLKMY TRPKR+  GK+L NE Q
Sbjct: 3   KKEKKANVSGKPKHSLDANRADGKKKTTETRSKSTVNRLKMYKTRPKRNAGGKILSNEYQ 62

Query: 64  SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDQQ 123
           SKELP++RI PDRRWFGNTRVVNQKELE+FREELQ +MS +YNVILKE+KLP+SLL D +
Sbjct: 63  SKELPNSRIAPDRRWFGNTRVVNQKELEYFREELQTKMSSNYNVILKERKLPMSLLTDNK 122

Query: 124 KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEEKHADNATAE-G 183
           KQSRVHLLD EPF+DAFG K KRKRPKL+A+DYE+L+KKA +S DAFEEK+    + E G
Sbjct: 123 KQSRVHLLDMEPFQDAFGRKTKRKRPKLVASDYEALVKKAAESQDAFEEKNGAGPSGEGG 182

Query: 184 SEGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 243
            E DGFRDLVRH MFEKGQSKRIWGELYKVIDSSDV+VQV+DARDPQGTRC+HLE+ LKE
Sbjct: 183 EEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVIVQVIDARDPQGTRCHHLEKTLKE 242

Query: 244 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFAR 303
           H KHKH++LLLNKCDL+PAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFAR
Sbjct: 243 HHKHKHMILLLNKCDLVPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFAR 302

Query: 304 LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP 363
           LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP
Sbjct: 303 LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP 362

Query: 364 GVVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLXYCRHATIMSWGDDNDF 423
           GVVYQ+ D+ETDIVLKGVVRVTNLEDASEHIGEVL+RVK+EHL   R   I  W DD+DF
Sbjct: 363 GVVYQSRDTETDIVLKGVVRVTNLEDASEHIGEVLRRVKKEHLQ--RAYKIKDWEDDHDF 422

Query: 424 LIQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDY---GVDD 483
           L+QLCK +GKLL+GGEPDL T AKM+LHDWQRGR+PFFVPPP++D+ + E +    G+D 
Sbjct: 423 LLQLCKSSGKLLKGGEPDLMTGAKMILHDWQRGRIPFFVPPPKLDNVASESEVIVPGIDK 482

Query: 484 DSGVDSNQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLS 543
           ++  D++QAAAA KAIA ++S+QQQ++VPVQRD + + +L  D   +        E+  +
Sbjct: 483 EAIADNSQAAAALKAIAGIMSTQQQKDVPVQRDFYDEKDLKDDKKAK--------ESTET 542

Query: 544 DTEDKTSGDQDD 549
           D E+ T  ++D+
Sbjct: 543 DAENGTDAEEDE 544

BLAST of MS003164 vs. ExPASy Swiss-Prot
Match: A2XGQ1 (Nuclear/nucleolar GTPase 2 OS=Oryza sativa subsp. indica OX=39946 GN=NUG2 PE=3 SV=1)

HSP 1 Score: 797.7 bits (2059), Expect = 8.2e-230
Identity = 411/536 (76.68%), Postives = 454/536 (84.70%), Query Frame = 0

Query: 1   MTKKKEKKVNVSGKPKHSLDVNRSDANKNS----------RSAATVRRLKMYNTRPKRDR 60
           M KKKE+ VNVSGKP+HSLDVNR++  K +          RSAATVRRLKMY  RP RDR
Sbjct: 1   MAKKKERAVNVSGKPRHSLDVNRANDKKGAGGGAGGGGGGRSAATVRRLKMYKMRPLRDR 60

Query: 61  KGKVLKNELQSKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKK 120
            GK+LK++LQSKELP+TRI+PDRRWFGNTRVVNQKELEFFREELQ R+S +YNVILKE+K
Sbjct: 61  GGKILKHDLQSKELPNTRIEPDRRWFGNTRVVNQKELEFFREELQSRLSNNYNVILKERK 120

Query: 121 LPLSLLNDQQKQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEEK 180
           LPLSLL D QKQ+R HLLDTEPFE AFGPKGKRKRPKL+A DYESLLKKAD S  AFE+K
Sbjct: 121 LPLSLLQDHQKQARAHLLDTEPFEHAFGPKGKRKRPKLMALDYESLLKKADDSQGAFEDK 180

Query: 181 HADNATAEGSEGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRC 240
           HA     +  E DG RDLVRH MFEKGQSKRIWGELYKVIDSSDVVVQVLDARDP GTRC
Sbjct: 181 HATAKLLKEEEEDGLRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPMGTRC 240

Query: 241 YHLERHLKEHCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSL 300
           YHLE+HLKE+ KHKH+V LLNKCDL+PAWATKGWLR LSK+YPTLAFHAS+N SFGKGSL
Sbjct: 241 YHLEKHLKENAKHKHLVFLLNKCDLVPAWATKGWLRTLSKDYPTLAFHASINSSFGKGSL 300

Query: 301 LSVLRQFARLKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLT 360
           LSVLRQFARLKSDKQAISVGFVGYPNVGKSSVINTLR+K+VCKVAPIPGETKVWQYITLT
Sbjct: 301 LSVLRQFARLKSDKQAISVGFVGYPNVGKSSVINTLRSKSVCKVAPIPGETKVWQYITLT 360

Query: 361 KRIFLIDCPGVVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLXYCRHATI 420
           KRIFLIDCPGVVYQN+DSETDIVLKGVVRVTNL DASEHIGEVL+RVK+EHL   R   I
Sbjct: 361 KRIFLIDCPGVVYQNNDSETDIVLKGVVRVTNLADASEHIGEVLRRVKKEHLK--RAYKI 420

Query: 421 MSWGDDNDFLIQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRV-DDKSEE 480
             W DDNDFL+QL K TGKLLRGGEPDLTT AKMVLHDWQRG++PFFVPPP+  +D   E
Sbjct: 421 EDWVDDNDFLVQLSKTTGKLLRGGEPDLTTTAKMVLHDWQRGKIPFFVPPPQQGEDSPSE 480

Query: 481 PDYGVD--DDSGVDSNQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQ 524
               VD  D+ GV S++ AAA KAIA +ISSQQQ NVP Q++ F  T  + + ++Q
Sbjct: 481 TAEPVDKSDEEGVSSDRTAAAMKAIAGIISSQQQMNVPCQKE-FGVTNEDSEVAEQ 533

BLAST of MS003164 vs. ExPASy Swiss-Prot
Match: Q10LF7 (Nuclear/nucleolar GTPase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=NUG2 PE=1 SV=1)

HSP 1 Score: 796.2 bits (2055), Expect = 2.4e-229
Identity = 410/536 (76.49%), Postives = 454/536 (84.70%), Query Frame = 0

Query: 1   MTKKKEKKVNVSGKPKHSLDVNRSDANKNS----------RSAATVRRLKMYNTRPKRDR 60
           M KKKE+ VNVSGKP+HSLDVNR++  K +          RSAATVRRLKMY  RP RDR
Sbjct: 1   MAKKKERAVNVSGKPRHSLDVNRANDKKGAGGGAGGGGGGRSAATVRRLKMYKMRPLRDR 60

Query: 61  KGKVLKNELQSKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKK 120
            GK+LK++LQSKELP+TRI+PDRRWFGNTRVVNQKELEFFREELQ R+S +YNVILKE+K
Sbjct: 61  GGKILKHDLQSKELPNTRIEPDRRWFGNTRVVNQKELEFFREELQSRLSNNYNVILKERK 120

Query: 121 LPLSLLNDQQKQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEEK 180
           LPLSLL D QKQ+R HLLDTEPFE AFGPKGKRKRPKL+A DYESLLKKAD S  AFE+K
Sbjct: 121 LPLSLLQDHQKQARAHLLDTEPFEHAFGPKGKRKRPKLMALDYESLLKKADDSQGAFEDK 180

Query: 181 HADNATAEGSEGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRC 240
           HA     +  E DG RDLVRH MFEKGQSKRIWGELYKVIDSSDVVVQVLDARDP GTRC
Sbjct: 181 HATAKLLKEEEEDGLRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPMGTRC 240

Query: 241 YHLERHLKEHCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSL 300
           YHLE+HLKE+ KHKH+V LLNKCDL+PAWATKGWLR LSK+YPTLAFHAS+N SFGKGSL
Sbjct: 241 YHLEKHLKENAKHKHLVFLLNKCDLVPAWATKGWLRTLSKDYPTLAFHASINSSFGKGSL 300

Query: 301 LSVLRQFARLKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLT 360
           LSVLRQFARLKSDKQAISVGFVGYPNVGKSSVINTLR+K+VCKVAPIPGETKVWQYITLT
Sbjct: 301 LSVLRQFARLKSDKQAISVGFVGYPNVGKSSVINTLRSKSVCKVAPIPGETKVWQYITLT 360

Query: 361 KRIFLIDCPGVVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLXYCRHATI 420
           KRIFLIDCPGVVYQN+DSETDIVLKGVVRVTNL DASEHIGEVL+RVK+EHL   R   I
Sbjct: 361 KRIFLIDCPGVVYQNNDSETDIVLKGVVRVTNLADASEHIGEVLRRVKKEHLK--RAYKI 420

Query: 421 MSWGDDNDFLIQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRV-DDKSEE 480
             W DDNDFL+QL K TGKLLRGGEPDLTT AKMVLHDWQRG++PFFVPPP+  +D   E
Sbjct: 421 EDWVDDNDFLVQLSKTTGKLLRGGEPDLTTTAKMVLHDWQRGKIPFFVPPPQQGEDSPSE 480

Query: 481 PDYGVD--DDSGVDSNQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQ 524
               V+  D+ GV S++ AAA KAIA +ISSQQQ NVP Q++ F  T  + + ++Q
Sbjct: 481 TAEPVEKSDEEGVSSDRTAAAMKAIAGIISSQQQMNVPCQKE-FGVTNEDSEVAEQ 533

BLAST of MS003164 vs. ExPASy Swiss-Prot
Match: Q13823 (Nucleolar GTP-binding protein 2 OS=Homo sapiens OX=9606 GN=GNL2 PE=1 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 1.9e-138
Identity = 257/472 (54.45%), Postives = 337/472 (71.40%), Query Frame = 0

Query: 1   MTKKKEKKVNVSGKPKHSLDVNR--SDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKN- 60
           M K K K  +     K S + +R      +N R  AT+RRL MY  + +R+ +GK++K  
Sbjct: 1   MVKPKYKGRSTINPSKASTNPDRVQGAGGQNMRDRATIRRLNMYRQKERRNSRGKIIKPL 60

Query: 61  ELQSKELPDT--RIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSL 120
           + QS     T  R++P+ +WFGNTRV+ Q  L+ F+EE+   M   Y V++K+ KLP+SL
Sbjct: 61  QYQSTVASGTVARVEPNIKWFGNTRVIKQSSLQKFQEEMDTVMKDPYKVVMKQSKLPMSL 120

Query: 121 LNDQQK--QSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEE-KHA 180
           L+D+ +    +VH+LDTE FE  FGPK +RKRP L A+D +SL++ A+ S +++++ K  
Sbjct: 121 LHDRIRPHNLKVHILDTESFETTFGPKSQRKRPNLFASDMQSLIENAEMSTESYDQGKDR 180

Query: 181 DNATAEGSEGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYH 240
           D  T    E  G R+  +  +++KGQSKRIWGELYKVIDSSDVVVQVLDARDP GTR  H
Sbjct: 181 DLVT----EDTGVRNEAQEEIYKKGQSKRIWGELYKVIDSSDVVVQVLDARDPMGTRSPH 240

Query: 241 LERHLKEHCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLS 300
           +E +LK+    KH++ +LNKCDL+P WATK W+ VLS++YPTLAFHAS+   FGKG+ + 
Sbjct: 241 IETYLKKEKPWKHLIFVLNKCDLVPTWATKRWVAVLSQDYPTLAFHASLTNPFGKGAFIQ 300

Query: 301 VLRQFARLKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKR 360
           +LRQF +L +DK+ ISVGF+GYPNVGKSSVINTLR+K VC VAPI GETKVWQYITL +R
Sbjct: 301 LLRQFGKLHTDKKQISVGFIGYPNVGKSSVINTLRSKKVCNVAPIAGETKVWQYITLMRR 360

Query: 361 IFLIDCPGVVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLXYCRHATIMS 420
           IFLIDCPGVVY + DSETDIVLKGVV+V  ++   +HIG VL+R K E++   +   I S
Sbjct: 361 IFLIDCPGVVYPSEDSETDIVLKGVVQVEKIKSPEDHIGAVLERAKPEYI--SKTYKIDS 420

Query: 421 WGDDNDFLIQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVD 465
           W +  DFL +L   TGKLL+GGEPDL T  KMVL+DWQRGR+PFFV PP  +
Sbjct: 421 WENAEDFLEKLAFRTGKLLKGGEPDLQTVGKMVLNDWQRGRIPFFVKPPNAE 466

BLAST of MS003164 vs. ExPASy Swiss-Prot
Match: Q99LH1 (Nucleolar GTP-binding protein 2 OS=Mus musculus OX=10090 GN=Gnl2 PE=1 SV=2)

HSP 1 Score: 488.8 bits (1257), Expect = 8.2e-137
Identity = 275/586 (46.93%), Postives = 374/586 (63.82%), Query Frame = 0

Query: 1   MTKKKEKKVNVSGKPKHSLDVNR--SDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKN- 60
           M K K K  +   +   S + +R      +N R   T+RRL MY  + +R+ +GKV+K  
Sbjct: 1   MVKPKYKGRSTINRSAASTNPDRVQGAGGQNMRDRGTIRRLNMYRQKERRNSRGKVIKPL 60

Query: 61  ELQSKELPDT--RIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSL 120
           + QS     T  R++P+ +WFGNTRV+ Q  L+ F+EE+ K M   Y V++K+ KLP+SL
Sbjct: 61  QYQSTVASGTVARVEPNIKWFGNTRVIKQASLQKFQEEMDKVMKDPYKVVMKQSKLPMSL 120

Query: 121 LND--QQKQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEEKHAD 180
           L+D  Q   ++VH+LDTE FE  FGPK +RKRP L A+D +SLL+ A+ S +++++    
Sbjct: 121 LHDRIQPHNAKVHILDTESFESTFGPKSQRKRPNLFASDMQSLLENAEMSTESYDQGKDR 180

Query: 181 NATAEGSEGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHL 240
           +   E +   G R+  +  +++KGQSKRIWGELYKVIDSSDVVVQVLDARDP GTR  H+
Sbjct: 181 DLVMEDT---GVRNEAQEEIYKKGQSKRIWGELYKVIDSSDVVVQVLDARDPMGTRSPHI 240

Query: 241 ERHLKEHCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSV 300
           E +LK+    KH++ +LNKCDL+P WATK W+ VLS++YPTLAFHAS+   FGKG+ + +
Sbjct: 241 EAYLKKEKPWKHLIFVLNKCDLVPTWATKRWVAVLSQDYPTLAFHASLTNPFGKGAFIQL 300

Query: 301 LRQFARLKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRI 360
           LRQF +L +DK+ ISVGF+GYPNVGKSSVINTLR+K VC VAPI GETKVWQYITL +RI
Sbjct: 301 LRQFGKLHTDKKQISVGFIGYPNVGKSSVINTLRSKKVCNVAPIAGETKVWQYITLMRRI 360

Query: 361 FLIDCPGVVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLXYCRHATIMSW 420
           FLIDCPGVVY + DSETDIVLKGVV+V  ++   +HIG VL+R K E++   +   I SW
Sbjct: 361 FLIDCPGVVYPSEDSETDIVLKGVVQVEKIKAPQDHIGAVLERAKPEYI--SKTYKIESW 420

Query: 421 GDDNDFLIQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYG 480
            +  DFL +L   TGKLL+GGEPD+ T +KMVL+DWQRGR+PFFV PP  +         
Sbjct: 421 ENAEDFLEKLALRTGKLLKGGEPDMLTVSKMVLNDWQRGRIPFFVKPPNAE--------- 480

Query: 481 VDDDSGVDSNQAAAAFKAIANVISSQQQRNVPVQR-DLFSDTELNGDAS-------DQIL 540
           +  DS +  +               ++     V+R D  ++ E  GD S        QIL
Sbjct: 481 LPTDSQLPPSSPLEVPTETTQNNPEEETTETEVERSDSITEKEPEGDCSQDRNSEMQQIL 540

Query: 541 V----------------SEDELEAPLSDTEDKTSGDQDDVVEDQRP 556
                            ++D +   +SD ED  S  +++  E ++P
Sbjct: 541 ARVRQNFGKINVGPQFSADDLVPVEMSDLEDLESSGEEEEQEQEQP 572

BLAST of MS003164 vs. ExPASy TrEMBL
Match: A0A6J1C0B4 (Nuclear/nucleolar GTPase 2 OS=Momordica charantia OX=3673 GN=LOC111006286 PE=3 SV=1)

HSP 1 Score: 1075.1 bits (2779), Expect = 0.0e+00
Identity = 547/558 (98.03%), Postives = 547/558 (98.03%), Query Frame = 0

Query: 1   MTKKKEKKVNVSGKPKHSLDVNRSDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ 60
           MTKKKEKKVNVSGKPKHSLDVNRSDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ
Sbjct: 1   MTKKKEKKVNVSGKPKHSLDVNRSDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ 60

Query: 61  SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDQQ 120
           SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLND Q
Sbjct: 61  SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDHQ 120

Query: 121 KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEEKHADNATAEGS 180
           KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEE HADNATAEGS
Sbjct: 121 KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEENHADNATAEGS 180

Query: 181 EGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH 240
           EGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH
Sbjct: 181 EGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH 240

Query: 241 CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARL 300
           CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARL
Sbjct: 241 CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARL 300

Query: 301 KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG 360
           KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG
Sbjct: 301 KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG 360

Query: 361 VVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLXYCRHATIMSWGDDNDFL 420
           VVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHL   R   I  W DDNDFL
Sbjct: 361 VVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLE--RAYKIKDWEDDNDFL 420

Query: 421 IQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSGV 480
           IQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSGV
Sbjct: 421 IQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSGV 480

Query: 481 DSNQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTED 540
           DSNQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTED
Sbjct: 481 DSNQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTED 540

Query: 541 KTSGDQDDVVEDQRPTAG 559
           KTSGDQDDVVEDQRPTAG
Sbjct: 541 KTSGDQDDVVEDQRPTAG 556

BLAST of MS003164 vs. ExPASy TrEMBL
Match: A0A6J1BWB7 (Nuclear/nucleolar GTPase 2 OS=Momordica charantia OX=3673 GN=LOC111006286 PE=3 SV=1)

HSP 1 Score: 1042.7 bits (2695), Expect = 5.4e-301
Identity = 535/558 (95.88%), Postives = 535/558 (95.88%), Query Frame = 0

Query: 1   MTKKKEKKVNVSGKPKHSLDVNRSDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ 60
           MTKKKEKKVNVSGKPKHSLDVNRSDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ
Sbjct: 1   MTKKKEKKVNVSGKPKHSLDVNRSDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ 60

Query: 61  SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDQQ 120
           SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLND Q
Sbjct: 61  SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDHQ 120

Query: 121 KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEEKHADNATAEGS 180
           KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSH             EGS
Sbjct: 121 KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSH-------------EGS 180

Query: 181 EGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH 240
           EGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH
Sbjct: 181 EGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH 240

Query: 241 CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARL 300
           CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARL
Sbjct: 241 CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARL 300

Query: 301 KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG 360
           KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG
Sbjct: 301 KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG 360

Query: 361 VVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLXYCRHATIMSWGDDNDFL 420
           VVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHL   R   I  W DDNDFL
Sbjct: 361 VVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLE--RAYKIKDWEDDNDFL 420

Query: 421 IQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSGV 480
           IQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSGV
Sbjct: 421 IQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSGV 480

Query: 481 DSNQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTED 540
           DSNQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTED
Sbjct: 481 DSNQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTED 540

Query: 541 KTSGDQDDVVEDQRPTAG 559
           KTSGDQDDVVEDQRPTAG
Sbjct: 541 KTSGDQDDVVEDQRPTAG 543

BLAST of MS003164 vs. ExPASy TrEMBL
Match: A0A5D3BS64 (Nuclear/nucleolar GTPase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G00410 PE=3 SV=1)

HSP 1 Score: 999.2 bits (2582), Expect = 6.9e-288
Identity = 507/558 (90.86%), Postives = 529/558 (94.80%), Query Frame = 0

Query: 1   MTKKKEKKVNVSGKPKHSLDVNRS-DANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNEL 60
           M KKKEKKVNVSGKPKHSLDVNRS + NKN RSAATVRRLKMYNTRPKRDRKGKVLKN+L
Sbjct: 1   MAKKKEKKVNVSGKPKHSLDVNRSNEKNKNGRSAATVRRLKMYNTRPKRDRKGKVLKNDL 60

Query: 61  QSKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDQ 120
           QS ELPDTRIQPDRRWFGNTRVVNQKELE FREEL+KRMS SYNVILKE+KLPLSLLND 
Sbjct: 61  QSNELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSLLNDH 120

Query: 121 QKQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEEKHADNATAEG 180
           QKQSRVHLLDTEPF+DAFGPKGKRKRPKLLAADYESLL+KADKSHD FEEK+A+NAT EG
Sbjct: 121 QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAENATVEG 180

Query: 181 SEGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 240
           SE DGFRDLVRH MFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE
Sbjct: 181 SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 240

Query: 241 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFAR 300
           HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHAS+NKSFGKGSLLSVLRQFAR
Sbjct: 241 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR 300

Query: 301 LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP 360
           LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP
Sbjct: 301 LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP 360

Query: 361 GVVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLXYCRHATIMSWGDDNDF 420
           GVVYQNSD+ETDIVLKGVVRVTNLEDASEHIGEVLKRVK+EHL   R   I +W DDNDF
Sbjct: 361 GVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLE--RAYKIKNWEDDNDF 420

Query: 421 LIQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSG 480
           L+QLCKLTGKLLRGGEPDLTTAAKMVLHDWQRG+LPFFVPPPRV+D+SEEP+YGVDDDSG
Sbjct: 421 LVQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGKLPFFVPPPRVEDESEEPNYGVDDDSG 480

Query: 481 VDSNQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTE 540
           VDSNQAAAAFKAIANVISSQQQR+VPVQRDLFS+ ELNG+ SDQ LVSEDEL+APLSDTE
Sbjct: 481 VDSNQAAAAFKAIANVISSQQQRSVPVQRDLFSENELNGETSDQTLVSEDELQAPLSDTE 540

Query: 541 DKTSGDQDDVVEDQRPTA 558
            KTSGDQDD  ED+   A
Sbjct: 541 GKTSGDQDDKNEDEHTIA 556

BLAST of MS003164 vs. ExPASy TrEMBL
Match: A0A6J1K2N0 (Nuclear/nucleolar GTPase 2 OS=Cucurbita maxima OX=3661 GN=LOC111489529 PE=3 SV=1)

HSP 1 Score: 992.6 bits (2565), Expect = 6.4e-286
Identity = 508/560 (90.71%), Postives = 531/560 (94.82%), Query Frame = 0

Query: 1   MTKKKEKKVNVSGKPKHSLDVNRSDANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ 60
           MTKK E+KV+VSGKPKHSLD NRSD NKN+RSAATVRRLKMYNTRPKRDRKGKVLK+ELQ
Sbjct: 1   MTKKTERKVSVSGKPKHSLDANRSDGNKNARSAATVRRLKMYNTRPKRDRKGKVLKHELQ 60

Query: 61  SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDQQ 120
           SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMS SYNVILKEKKLPLSLLND Q
Sbjct: 61  SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSSSYNVILKEKKLPLSLLNDHQ 120

Query: 121 KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEEKHADNATAEGS 180
           KQSRVHLLDTEPF+DAFGPKGKRKRPKLLAADYESLLKKADKSHD F+EK+A+NAT EGS
Sbjct: 121 KQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLKKADKSHDDFDEKYAENATNEGS 180

Query: 181 EGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH 240
           E DGFRDLVRH MFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH
Sbjct: 181 EEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEH 240

Query: 241 CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARL 300
           CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHAS+NKSFGKGSLLSVLRQFARL
Sbjct: 241 CKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFARL 300

Query: 301 KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG 360
           KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG
Sbjct: 301 KSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPG 360

Query: 361 VVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLXYCRHATIMSWGDDNDFL 420
           VVYQNSD+ETD+VLKGVVRVTNLED SEHIGEVLKRVK+EHL   R   I +W DDNDFL
Sbjct: 361 VVYQNSDTETDVVLKGVVRVTNLEDPSEHIGEVLKRVKKEHLE--RAYKIKNWVDDNDFL 420

Query: 421 IQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKS-EEPDYGVDDDSG 480
           +QLCKL+GKLLRGGEPDLTT AKMVLHDWQRG+LPFFVPPPRV+DKS EEP+Y VDDDSG
Sbjct: 421 VQLCKLSGKLLRGGEPDLTTVAKMVLHDWQRGKLPFFVPPPRVEDKSEEEPNYSVDDDSG 480

Query: 481 VDSNQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDA-SDQILVSEDELEAPLSDT 540
           VDSNQAAAAFKAIANVISSQQQR+VPVQRDLFSD ELNG++  DQILVSEDEL+AP SDT
Sbjct: 481 VDSNQAAAAFKAIANVISSQQQRSVPVQRDLFSDNELNGESCDDQILVSEDELQAP-SDT 540

Query: 541 EDKTSGDQDDVVEDQRPTAG 559
           E +TS  QDDVVED RPTAG
Sbjct: 541 EGRTSEGQDDVVEDVRPTAG 557

BLAST of MS003164 vs. ExPASy TrEMBL
Match: A0A0A0KTZ3 (Nuclear/nucleolar GTPase 2 OS=Cucumis sativus OX=3659 GN=Csa_5G631500 PE=3 SV=1)

HSP 1 Score: 988.4 bits (2554), Expect = 1.2e-284
Identity = 501/554 (90.43%), Postives = 526/554 (94.95%), Query Frame = 0

Query: 1   MTKKKEKKVNVSGKPKHSLDVNRS-DANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNEL 60
           M KKKEKKVNVSGKPKHSLDVNRS + NKN R+AATVRRLKMYNTRPKRDRKGKVLKN+L
Sbjct: 1   MAKKKEKKVNVSGKPKHSLDVNRSNEKNKNGRTAATVRRLKMYNTRPKRDRKGKVLKNDL 60

Query: 61  QSKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDQ 120
           QS ELPDTRIQPDRRWFGNTRVVNQKELE FREEL+KRMS SYNVILKE+KLPLSLLND 
Sbjct: 61  QSNELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSLLNDH 120

Query: 121 QKQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEEKHADNATAEG 180
           QKQSRVHLLDTEPF+DAFGPKGKRKRPKLLAADYESLL+KADKSHD FEEK+A+NAT EG
Sbjct: 121 QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAENATVEG 180

Query: 181 SEGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 240
           SE DGFRDLVRH MFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE
Sbjct: 181 SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 240

Query: 241 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFAR 300
           HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHAS+NKSFGKGSLLSVLRQFAR
Sbjct: 241 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR 300

Query: 301 LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP 360
           LKSDKQAISVGFVGYPNVGKSSVINTLRTK VCKVAPIPGETKVWQYITLTKRIFLIDCP
Sbjct: 301 LKSDKQAISVGFVGYPNVGKSSVINTLRTKTVCKVAPIPGETKVWQYITLTKRIFLIDCP 360

Query: 361 GVVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLXYCRHATIMSWGDDNDF 420
           GVVYQNSD+ETDIVLKGVVRVTNLEDASEHIGEVLKRVK+EHL   R   I +W DDNDF
Sbjct: 361 GVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLE--RAYKIKNWEDDNDF 420

Query: 421 LIQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDYGVDDDSG 480
           L+QLCKL+GKLL+GGEPDLTTAAKMVLHDWQRG+LPFFVPPPRV+D+SEEP+Y VDDDSG
Sbjct: 421 LVQLCKLSGKLLKGGEPDLTTAAKMVLHDWQRGKLPFFVPPPRVEDESEEPNYCVDDDSG 480

Query: 481 VDSNQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLSDTE 540
           VDSNQAAAAFKAIANVISSQQQR+VPVQRDLFS+ ELNG+ SDQILVSEDEL+APLSDTE
Sbjct: 481 VDSNQAAAAFKAIANVISSQQQRSVPVQRDLFSENELNGETSDQILVSEDELQAPLSDTE 540

Query: 541 DKTSGDQDDVVEDQ 554
            KT GDQDD  ED+
Sbjct: 541 GKTLGDQDDKNEDE 552

BLAST of MS003164 vs. TAIR 10
Match: AT1G52980.1 (GTP-binding family protein )

HSP 1 Score: 799.3 bits (2063), Expect = 2.0e-231
Identity = 406/552 (73.55%), Postives = 469/552 (84.96%), Query Frame = 0

Query: 4   KKEKKVNVSGKPKHSLDVNRSDANK---NSRSAATVRRLKMYNTRPKRDRKGKVLKNELQ 63
           KKEKK NVSGKPKHSLD NR+D  K    +RS +TV RLKMY TRPKR+  GK+L NE Q
Sbjct: 3   KKEKKANVSGKPKHSLDANRADGKKKTTETRSKSTVNRLKMYKTRPKRNAGGKILSNEYQ 62

Query: 64  SKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDQQ 123
           SKELP++RI PDRRWFGNTRVVNQKELE+FREELQ +MS +YNVILKE+KLP+SLL D +
Sbjct: 63  SKELPNSRIAPDRRWFGNTRVVNQKELEYFREELQTKMSSNYNVILKERKLPMSLLTDNK 122

Query: 124 KQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEEKHADNATAE-G 183
           KQSRVHLLD EPF+DAFG K KRKRPKL+A+DYE+L+KKA +S DAFEEK+    + E G
Sbjct: 123 KQSRVHLLDMEPFQDAFGRKTKRKRPKLVASDYEALVKKAAESQDAFEEKNGAGPSGEGG 182

Query: 184 SEGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 243
            E DGFRDLVRH MFEKGQSKRIWGELYKVIDSSDV+VQV+DARDPQGTRC+HLE+ LKE
Sbjct: 183 EEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVIVQVIDARDPQGTRCHHLEKTLKE 242

Query: 244 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFAR 303
           H KHKH++LLLNKCDL+PAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFAR
Sbjct: 243 HHKHKHMILLLNKCDLVPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFAR 302

Query: 304 LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP 363
           LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP
Sbjct: 303 LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP 362

Query: 364 GVVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLXYCRHATIMSWGDDNDF 423
           GVVYQ+ D+ETDIVLKGVVRVTNLEDASEHIGEVL+RVK+EHL   R   I  W DD+DF
Sbjct: 363 GVVYQSRDTETDIVLKGVVRVTNLEDASEHIGEVLRRVKKEHLQ--RAYKIKDWEDDHDF 422

Query: 424 LIQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLPFFVPPPRVDDKSEEPDY---GVDD 483
           L+QLCK +GKLL+GGEPDL T AKM+LHDWQRGR+PFFVPPP++D+ + E +    G+D 
Sbjct: 423 LLQLCKSSGKLLKGGEPDLMTGAKMILHDWQRGRIPFFVPPPKLDNVASESEVIVPGIDK 482

Query: 484 DSGVDSNQAAAAFKAIANVISSQQQRNVPVQRDLFSDTELNGDASDQILVSEDELEAPLS 543
           ++  D++QAAAA KAIA ++S+QQQ++VPVQRD + + +L  D   +        E+  +
Sbjct: 483 EAIADNSQAAAALKAIAGIMSTQQQKDVPVQRDFYDEKDLKDDKKAK--------ESTET 542

Query: 544 DTEDKTSGDQDD 549
           D E+ T  ++D+
Sbjct: 543 DAENGTDAEEDE 544

BLAST of MS003164 vs. TAIR 10
Match: AT3G07050.1 (GTP-binding family protein )

HSP 1 Score: 199.1 bits (505), Expect = 9.2e-51
Identity = 127/398 (31.91%), Postives = 209/398 (52.51%), Query Frame = 0

Query: 180 SEGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 239
           +EG+   DL +         +  + EL KVI+ SDV+++VLDARDP GTRC  +ER + +
Sbjct: 104 TEGETIEDLPKVVNVRDNSERAFYKELVKVIELSDVILEVLDARDPLGTRCTDMERMVMQ 163

Query: 240 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKS--------------- 299
              +KH+VLLLNK DL+P  A + WL  L +E+P +AF  S  +                
Sbjct: 164 AGPNKHLVLLLNKIDLVPREAAEKWLMYLREEFPAVAFKCSTQEQRSNLGWKSSKASKPS 223

Query: 300 --------FGKGSLLSVLRQFARLKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAP 359
                    G  +L+ +L+ ++R    K++I+VG +G PNVGKSS+IN+L+  +V  V  
Sbjct: 224 NMLQTSDCLGADTLIKLLKNYSRSHELKKSITVGIIGLPNVGKSSLINSLKRAHVVNVGA 283

Query: 360 IPGETKVWQYITLTKRIFLIDCPGVV-YQNSDSETDIVLKGVVRVTNLEDASEHIGEVLK 419
            PG T+  Q + L K + L+DCPGVV  ++S ++  I L+   R+  L+D    + E+LK
Sbjct: 284 TPGLTRSLQEVHLDKNVKLLDCPGVVMLKSSGNDASIALRNCKRIEKLDDPVSPVKEILK 343

Query: 420 RVKREHLXYCRHATIMSWGDDNDFLIQLCKLTGKLLRGGEPDLTTAAKMVLHDWQRGRLP 479
              ++ L       I S+   +DFL ++  + GKL +GG  D+  AA++VLHDW  G++P
Sbjct: 344 LCPKDMLVTL--YKIPSFEAVDDFLYKVATVRGKLKKGGLVDIDAAARIVLHDWNEGKIP 403

Query: 480 FFVPPPRVDDKSEEPDYGVDD---DSGVDS-NQAAAAFKAIANVISSQQQRNVPVQRDL- 539
           ++  PP+ D         V +   D  +D      ++F      ++      +P    L 
Sbjct: 404 YYTMPPKRDQGGHAESKIVTELAKDFNIDEVYSGESSFIGSLKTVNEFNPVIIPSNGPLN 463

Query: 540 FSDTELNGDASDQILVSEDELEAPLSDTEDKTSGDQDD 549
           F +T +  ++  Q   +E+E E   SD ++   G++++
Sbjct: 464 FDETMIEDESKTQ---TEEEAEHE-SDDDESMGGEEEE 495

BLAST of MS003164 vs. TAIR 10
Match: AT2G27200.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 144.1 bits (362), Expect = 3.5e-34
Identity = 101/352 (28.69%), Postives = 168/352 (47.73%), Query Frame = 0

Query: 194 FEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEHCKHKHVVLLLNKC 253
           FEK     IW +L++V++ SD++V V+DARDP   RC  LE + +E  +HK  +LL+NK 
Sbjct: 151 FEKNLD--IWRQLWRVLERSDLIVMVVDARDPLFYRCPDLEAYAQEIDEHKKTMLLVNKA 210

Query: 254 DLIPAWATKGWLRVLSKEYPTLAFHAS----------------------------VNKSF 313
           DL+P++  + W    S+      F ++                              K +
Sbjct: 211 DLLPSYVREKWAEYFSRNNILFVFWSAKAATATLEGKPLKEQWRAPDTTQKTDNPAVKVY 270

Query: 314 GKGSLLSVLR----QFARLKSDK-----------QAISVGFVGYPNVGKSSVINTLRTKN 373
           G+  LL  L+    +  +++  +           + + VGFVGYPNVGKSS IN L  + 
Sbjct: 271 GRDDLLDRLKLEALEIVKMRKSRGVSATSTESHCEQVVVGFVGYPNVGKSSTINALVGQK 330

Query: 374 VCKVAPIPGETKVWQYITLTKRIFLIDCPGVVYQN-SDSETDIVLKGVVRVTNLEDASEH 433
              V   PG+TK +Q + +++ + L DCPG+V+ + S S  ++V  GV+ +  + +  E 
Sbjct: 331 RTGVTSTPGKTKHFQTLIISEDLMLCDCPGLVFPSFSSSRYEMVASGVLPIDRMTEHLEA 390

Query: 434 IGEVLKRVKREHLXYCRHATI---MSWGDD------NDFLIQLCKLTGKLLRGGEPDLTT 491
           I  V + V R  +    + ++    S+         ++ L   C   G +   G PD T 
Sbjct: 391 IKVVAELVPRHAIEDVYNISLPKPKSYEPQSRPPLASELLRTYCLSRGYVASSGLPDETR 450

BLAST of MS003164 vs. TAIR 10
Match: AT1G08410.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 136.0 bits (341), Expect = 9.5e-32
Identity = 94/335 (28.06%), Postives = 160/335 (47.76%), Query Frame = 0

Query: 194 FEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEHCKHKHVVLLLNKC 253
           FEK     IW +L++V++ SD++V V+DARDP   RC  LE + +E  +HK ++LL+NK 
Sbjct: 151 FEKNLD--IWRQLWRVLERSDLIVMVVDARDPLFYRCPDLEAYAQEIDEHKKIMLLVNKA 210

Query: 254 DLIPAWATKGWLRVLSKEYPTLAFHASVNKS----------------------------F 313
           DL+P    + W            F +++  +                            +
Sbjct: 211 DLLPTDVREKWAEYFRLNNILFVFWSAIAATATLEGKVLKEQWRQPDNLQKTDDPDIMIY 270

Query: 314 GKGSLLSVL----RQFARLKSDKQA---------------ISVGFVGYPNVGKSSVINTL 373
           G+  LLS L    ++  ++++ + A                 VGFVGYPNVGKSS IN L
Sbjct: 271 GRDELLSRLQFEAQEIVKVRNSRAASVSSQSWTGEYQRDQAVVGFVGYPNVGKSSTINAL 330

Query: 374 RTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPGVVYQN-SDSETDIVLKGVVRVTNLED 433
             +    V   PG+TK +Q + ++  + L DCPG+V+ + S S  +++  GV+ +  + +
Sbjct: 331 VGQKRTGVTSTPGKTKHFQTLIISDELMLCDCPGLVFPSFSSSRYEMIASGVLPIDRMTE 390

Query: 434 ASEHIGEVLKRVKREHLXYCRHATI---MSWGDDN------DFLIQLCKLTGKLLRGGEP 472
             E I  V  +V R  +    + ++    ++   +      + L   C   G +   G P
Sbjct: 391 HREAIQVVADKVPRRVIESVYNISLPKPKTYERQSRPPHAAELLKSYCASRGYVASSGLP 450

BLAST of MS003164 vs. TAIR 10
Match: AT4G02790.1 (GTP-binding family protein )

HSP 1 Score: 83.6 bits (205), Expect = 5.6e-16
Identity = 70/270 (25.93%), Postives = 122/270 (45.19%), Query Frame = 0

Query: 205 ELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEHCKHKHVVLLLNKCDLIPAWATKGW 264
           EL + +   DVV++V DAR P  T    ++  L     ++  +L+LN+ D+I       W
Sbjct: 109 ELREQLKLMDVVIEVRDARIPLSTTHPKMDAWL----GNRKRILVLNREDMISNDDRNDW 168

Query: 265 LRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFARLKSD-----------KQAISVGFVG 324
            R  +K+   + F    N   G G+ + + R    L  D            +++  G +G
Sbjct: 169 ARYFAKQGIKVIF---TNGKLGMGA-MKLGRLAKSLAGDVNGKRREKGLLPRSVRAGIIG 228

Query: 325 YPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPGVVYQNSDSETDIV 384
           YPNVGKSS+IN L  + +C  AP PG T+  +++ L K + L+D PG++    D +   +
Sbjct: 229 YPNVGKSSLINRLLKRKICAAAPRPGVTREMKWVKLGKDLDLLDSPGMLPMRIDDQAAAI 288

Query: 385 LKGVV-----RVTNLEDASEHIGEVLKRVKR--EHLXYCRHATIMSWGDDNDFLIQLCKL 444
              +      +  +  D +  + ++L R+        Y R+   +       F+    K 
Sbjct: 289 KLAICDDIGEKAYDFTDVAGILVQMLARIPEVGAKALYNRYKIQLEGNCGKKFV----KT 348

Query: 445 TGKLLRGGEPDLTTAAKMVLHDWQRGRLPF 457
            G  L GG  D   AA  +L D+++G+  +
Sbjct: 349 LGLNLFGG--DSHQAAFRILTDFRKGKFGY 364

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022133818.10.0e+0098.03nuclear/nucleolar GTPase 2 isoform X1 [Momordica charantia][more]
XP_022133820.11.1e-30095.88nuclear/nucleolar GTPase 2 isoform X2 [Momordica charantia][more]
XP_038890331.17.5e-28991.04nuclear/nucleolar GTPase 2 [Benincasa hispida] >XP_038890332.1 nuclear/nucleolar... [more]
TYK01950.11.4e-28790.86nuclear/nucleolar GTPase 2 [Cucumis melo var. makuwa][more]
XP_022993572.11.3e-28590.71nuclear/nucleolar GTPase 2 [Cucurbita maxima] >XP_022993573.1 nuclear/nucleolar ... [more]
Match NameE-valueIdentityDescription
Q9C9232.8e-23073.55Nuclear/nucleolar GTPase 2 OS=Arabidopsis thaliana OX=3702 GN=NUG2 PE=1 SV=1[more]
A2XGQ18.2e-23076.68Nuclear/nucleolar GTPase 2 OS=Oryza sativa subsp. indica OX=39946 GN=NUG2 PE=3 S... [more]
Q10LF72.4e-22976.49Nuclear/nucleolar GTPase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=NUG2 PE=1... [more]
Q138231.9e-13854.45Nucleolar GTP-binding protein 2 OS=Homo sapiens OX=9606 GN=GNL2 PE=1 SV=1[more]
Q99LH18.2e-13746.93Nucleolar GTP-binding protein 2 OS=Mus musculus OX=10090 GN=Gnl2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1C0B40.0e+0098.03Nuclear/nucleolar GTPase 2 OS=Momordica charantia OX=3673 GN=LOC111006286 PE=3 S... [more]
A0A6J1BWB75.4e-30195.88Nuclear/nucleolar GTPase 2 OS=Momordica charantia OX=3673 GN=LOC111006286 PE=3 S... [more]
A0A5D3BS646.9e-28890.86Nuclear/nucleolar GTPase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A6J1K2N06.4e-28690.71Nuclear/nucleolar GTPase 2 OS=Cucurbita maxima OX=3661 GN=LOC111489529 PE=3 SV=1[more]
A0A0A0KTZ31.2e-28490.43Nuclear/nucleolar GTPase 2 OS=Cucumis sativus OX=3659 GN=Csa_5G631500 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G52980.12.0e-23173.55GTP-binding family protein [more]
AT3G07050.19.2e-5131.91GTP-binding family protein [more]
AT2G27200.13.5e-3428.69P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT1G08410.19.5e-3228.06P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT4G02790.15.6e-1625.93GTP-binding family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 150..170
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 498..520
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..24
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 498..557
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 456..479
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..66
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 535..557
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 51..66
NoneNo IPR availablePANTHERPTHR11089GTP-BINDING PROTEIN-RELATEDcoord: 3..548
NoneNo IPR availableCDDcd01858NGP_1coord: 205..361
e-value: 5.29232E-118
score: 343.512
IPR006073GTP binding domainPRINTSPR00326GTP1OBGcoord: 355..370
score: 26.08
coord: 309..329
score: 46.14
IPR006073GTP binding domainPFAMPF01926MMR_HSR1coord: 309..368
e-value: 2.7E-14
score: 53.2
IPR012971Nucleolar GTP-binding protein 2, N-terminal domainPFAMPF08153NGP1NTcoord: 41..166
e-value: 6.3E-47
score: 159.0
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 193..363
e-value: 1.2E-44
score: 154.0
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 199..393
IPR023179GTP-binding protein, orthogonal bundle domain superfamilyGENE3D1.10.1580.10coord: 364..457
e-value: 1.1E-11
score: 46.6
IPR024929Nucleolar GTP-binding protein 2PANTHERPTHR11089:SF9NUCLEOLAR GTP-BINDING PROTEIN 2coord: 3..548
IPR030378Circularly permuted (CP)-type guanine nucleotide-binding (G) domainPROSITEPS51721G_CPcoord: 203..364
score: 37.170853

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS003164.1MS003164.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005730 nucleolus
molecular_function GO:0005525 GTP binding