MS002753 (gene) Bitter gourd (TR) v1

Overview
NameMS002753
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionRING-type domain-containing protein
Locationscaffold66: 295826 .. 300457 (+)
RNA-Seq ExpressionMS002753
SyntenyMS002753
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGCAGAGAATACCGCGCACTAGTCAGATGGTCGATATGGAAATGGATCGACAAGGTCAGAATTATCTTCATGCTGAGCCTTCCATCATTATGCCAGGCCCATCTAACTTCCCTCAGCATAGTATTCATTCAATGGTAACAGCTTCTGGGAATCCCGAGGCCCATTATCTACCCGATCCTTATGATGGCTCCATGTTACATGGTTTAAATCAGTATGGTAGCGTTCAGCATCATCACAGTCTAGGTTTGAGTACTGCAGCCCCTGGAAACTATTATTACTCTTACGTTACACCGCCATCAAGTAATGGGATGTTACCTGCACCTCTAAATCATAACGTGTCCGAGCAGTTGCCATCTTCTAGCAATTATGGAGTCATTCAAACGTCTTCTGATGGTTATGGAAGGAATTCATATTTTGTGGATGAAGTTAGTGATCCGCGTAAGAGAAAAATAACAGAAGGGATTCCTGGGAACATTCAACACATGAATGGTGTGGCCAGCTCTAGTTCTTCTGTGCATTTGTCGAATTCAAGGCATCCTGACGAGGTTGCTATGGTGGAAGCATCATCTTTTCCCCTGCCTCAGAGTAGATGGAATGGACCGCGGAGTAGTGTTAGAGCTGCATCAGGTGGAACTAGGCGGGACTCTCTTCTACCACCCGAACACAATCATTCAACTCTAGGAAATAATAGGGGTCAACACCTTCAGCCCGCTAATTCTAGTTTCTGGTTGGACCAACACTTACAGGCTAATTGTGGAAACGGAAGTGCTTCATCTTGGAACCAGACGTCTACCCCACCTTTCATGCACGGTATATTTTCCAATATCTATTCTCGTTTTGAATTAGCTCTCTTGCATTAGTGTTCGTCATGTTCTGTTGGACAGAATTTTTTTCGCAGCATGCTGTTTTTGGGATGAAATTTCTTTCTAACTTCCTTTGTTGTGCCCTTTTTTAGTGGTCATGAATAGAACATAGAATTAGAAGGTATTTGAGTGGTGGCTATTTATGTTAAAATTTTATTTTAGGTGCTACTTGGTAATTTGGATGTATTTTCTAGGTATTTGAAAAAGGAATTCCTATGCTGTCTGTGGGTTGTTGTCTCTGTTTTCTTGTTGTTTTTGAGAACATTTGAACGTGGTTATTTTTTTATTTTTTCTTTTCTTCTTTCTTTCTTTTTAAGTGTAGGATTATGTTTTTACGTCATTCCCAAACAACACCTCAATCTTTAATGGTTCTGAGCCAAATTAAGGAAATGCATATATACATTACTGAATTTTGAAATCTCTTACAGGAACTAATACAAATGGAGGTTTGTTGGAAACCATGAATTTGGGTGTGCATAGATATCATGAAACGGCGGGGAACCGGAGTTCTAGAAATGTCCAACATCCTTCAGTTAACCATGGCCACCACATTCATAATCATCCATCTGCAGTTGTGCAAAGAATTAGAGGCCACAATTTCCAGTTCTACCCCCAAATGACAGCAGCTTCATATGGATTTCCTTTGAATTCTTCATATGCCACCATGAATCCTCATAGTTTGGAGATAGGGCGTAGGCAACCAGGACCAGTTCCACCTACCGGTCACGGGCTGCACCGGCTTCCACGAGCGAATGTTGCTGCTGACACTACAACTAGACATCATAATGTTCCTCAGTTGAGATTCTTGCAGACTGATGTAATATCTCTTTCCCCTAGCATACCACTTTTTTTTTTTTTAATACCCCATTGAAGTAGCTTAAGAAATGAGCAAACTGGTATTTACAGGAAGTTGCATTATTGGAGATTCCGGAATTCTACGAAGTGGGGAATTTTGTTGACCATCACAGGGACATGCGATTGGATATAGAGGATATGTCTTACGAGGTGCGTTATTGATCATATTTTTTAAATCGAAACGAATACACTGGCACTCCATATTTGTCTAATCTTTGAGCTTTATTATTATTATTTTTAGCATGTAGACATAAGAAACATGTGAGGTAAATGTAACACATTTTCAATTAACAAAATTATACTCGTTTCTTGAAGAATATAACTTGAAGTATTTAGATAAATGATTATTAGGCATTGGATTCTTTCCCCCTTCAAACTCTTTGTTTTAAGACATTACATAACTAGGAATATGTAGTTATCGTTCTTCAACTACTTGTAAATATGTGTATTTTTATTCTCTTAGTTTCTTGTATACAAAGTCCAAACTTTACCAAAATTGTTGGAATATGTTTTAAAATAAGTTTAAATTCTATCTCGGTCTTGAACTTTCATACTTTGTTTTGCTTTGATCTCTCAACTGTCTTGCTCTGTTTTAGTTCCCAAGTTAGCATGAAAAACTATTTTAGTCCCTATTTAAATTCATTTATAAAAATTTCGTGTATTAATTGATAAAGTAGATGAACAAAAAAATGTACTGATGTCATATGTCTAATTAGAAAATAAATATCTAGGACTAATATGGTTTTTGCTCCTCGAGGTAGGAACCAAAATAGAATAAATGTGAAAATTTAGGGAGCGAAATAGGATTTAAACCTTAAAGTATAGAAATTTTAGTAAATATGGCCTTGTTAGCGATTTTAAAGCGCATGACACACTTCTATTGGACAAGTCTTTTGATTACCTAACGGCAAAAGGTGATCAAAAGGAATTGCCGGGGCGAAGCGATGAATAAATAAATTCATGATATTATTTATTGGGAGGGATGATGTGAGGGTGTGGACCCCCAATCCTTCGAAAGGTTTTTCTTGTAGCTCATTTTTCCATGTCCTGGACTCCTCATCATCCTCTTCTTCTTCTCCGTCCCTGTTGACCCCTTGGTTCACCTCGCTTTGGAAGATTAAAATTCCAAAGAAGATTAAGTTCTCTGGGTGGCAAGTCTTACTTGAAAAAGTTAATACCTTGGATTGGATCTAGAGGATGTCCTCCCTTTGTTTGAGTCCGCAGTGGTGTGTTCTGTGTAGGAATGCTTCGGAGGATCTTGATCATTTGTTGTGGTCTTGTCAGTTCGCATAGGAGTTATGGCTTCGTTTCTTTAGATGTTTTGGGATTGTTTGGGCCTGTTCTAGGGATTGTAGGGCGATGATGGAGGAAGTATTGTTATTCCCCCCGTTTCGAGACAAAGGACGTTTTCTTTAGCAAGCGTGTTTTTTGGCTACCCTATGGGGTATTTGGCAGGAGAGAAACAATATAATTTTTAGGGGAGTGGAGAGATCTGTGGATTATGTGTGGGATCTTATTAGATTTAACACTTCTCTATGGGGATCGGTCACTAAGGCTTTTTGTAATTATCAGTTAGGAGTCATACTTTTGGATTGGAGCCCGTTTCTTTTGGGGTTTCCTTTTGCTGGGCTATTTTTTTGTATGCCCTCTTATTGTAGTCTTTATTCATTTATCTCAATGAAAGAGCTGTTATTTATCCAAAAAAAAAAAAAAATTCATGATATTATATATAGTGAGAACTTGTAAGAAAACTAGATTACATGGGAAAGGAATAGAAGAATGATAAGAAAATTTTGTAAAAAGAATTGGAGGGTCCACAATCCACTATCCCCAAGCATGATATCAAGATGATTTTAAAATTATGGGGAACAAGAAATAAATAAGAAGATACCATAAGCAGATGTCCTGTTATAGAAAATCAATGGAGTCAGAAAGTTGAAGAGCAGAAGCTTCTGCAAAAGAAGGGCAGAAAAATGGCTGACATTTGAGTGATCAAACGCTGGGATAAAATCATCGGCTGAACTTGAAGGCAAAGACGGAAGGAGAAGCCATGATTGAAGAACAAAGAAGGATGAAATAGCTGAACTAAAAGAAAGAAAGGAATTGTTGAGCTAAATAGATGAAGGCTGAAGTTGAAGGAAGCAAAAGCAAGGGTTAGAGACCAAAGAAGAAAGAAATTGCTGAACTAAAAAATTGAAGGCTGAAGTTGACGAAGGAAGACGCCAAGATTGAAGAAAGGTGAAGATGGTGAGCCCCCCCTCTTAGGGAAGCTGCTCTCCTTGTCGCCTCTCGCCTTGAAAACGCTAGACCTTGTGTTAATATCTTGAGTTTTTGAAAGATGGCAGGTCACTGTGTCCCATATTTATAATTGTGGTAGTTAGCTGCTAGAATGAATTGATTTTGTTGATTAAATATGGAGATTGAATCGATTTAGACGAATACAGGGTACATAATAAATCACTGTCTATTTAAAGCTCTAACACAAGAAAGGGTGTTTATACAGGAACTTCTCGCTTTAGGGGAGCGAATTGGTAACGTCAGCACTGGGTTAACAGAGGAAATCATTAAAACTCAATTAAAAACAAGGAGCTACGTATCATCTGCAACAGTTGTTAATCTGGAGGAAGAGGGTTCAAGTTTGGACCAGGATGCCGATTACTGTATCATCTGCCAGGTAAAAATAAATAGTCTCTCCATATTTGCATGCTTACTTCCGAGTTTCATCTTTCAAGTTTGGTCCTCATACGAAGCAACATCGGTTTACAGGACCACTATCAGAACCGGGAGAAAGTTGGAATGCTCAATTGTGGACATGAGTACCACGCGAGTTGCTTAAAGAAATGGCTGCTCGTCAAGAATGTCTGTCCCGTCTGCAAATCGGAAGCATTGGCGACGGATAGAAAGGAACGA

mRNA sequence

ATGAGGCAGAGAATACCGCGCACTAGTCAGATGGTCGATATGGAAATGGATCGACAAGGTCAGAATTATCTTCATGCTGAGCCTTCCATCATTATGCCAGGCCCATCTAACTTCCCTCAGCATAGTATTCATTCAATGGTAACAGCTTCTGGGAATCCCGAGGCCCATTATCTACCCGATCCTTATGATGGCTCCATGTTACATGGTTTAAATCAGTATGGTAGCGTTCAGCATCATCACAGTCTAGGTTTGAGTACTGCAGCCCCTGGAAACTATTATTACTCTTACGTTACACCGCCATCAAGTAATGGGATGTTACCTGCACCTCTAAATCATAACGTGTCCGAGCAGTTGCCATCTTCTAGCAATTATGGAGTCATTCAAACGTCTTCTGATGGTTATGGAAGGAATTCATATTTTGTGGATGAAGTTAGTGATCCGCGTAAGAGAAAAATAACAGAAGGGATTCCTGGGAACATTCAACACATGAATGGTGTGGCCAGCTCTAGTTCTTCTGTGCATTTGTCGAATTCAAGGCATCCTGACGAGGTTGCTATGGTGGAAGCATCATCTTTTCCCCTGCCTCAGAGTAGATGGAATGGACCGCGGAGTAGTGTTAGAGCTGCATCAGGTGGAACTAGGCGGGACTCTCTTCTACCACCCGAACACAATCATTCAACTCTAGGAAATAATAGGGGTCAACACCTTCAGCCCGCTAATTCTAGTTTCTGGTTGGACCAACACTTACAGGCTAATTGTGGAAACGGAAGTGCTTCATCTTGGAACCAGACGTCTACCCCACCTTTCATGCACGGAACTAATACAAATGGAGGTTTGTTGGAAACCATGAATTTGGGTGTGCATAGATATCATGAAACGGCGGGGAACCGGAGTTCTAGAAATGTCCAACATCCTTCAGTTAACCATGGCCACCACATTCATAATCATCCATCTGCAGTTGTGCAAAGAATTAGAGGCCACAATTTCCAGTTCTACCCCCAAATGACAGCAGCTTCATATGGATTTCCTTTGAATTCTTCATATGCCACCATGAATCCTCATAGTTTGGAGATAGGGCGTAGGCAACCAGGACCAGTTCCACCTACCGGTCACGGGCTGCACCGGCTTCCACGAGCGAATGTTGCTGCTGACACTACAACTAGACATCATAATGTTCCTCAGTTGAGATTCTTGCAGACTGATGAAGTTGCATTATTGGAGATTCCGGAATTCTACGAAGTGGGGAATTTTGTTGACCATCACAGGGACATGCGATTGGATATAGAGGATATGTCTTACGAGGAACTTCTCGCTTTAGGGGAGCGAATTGGTAACGTCAGCACTGGGTTAACAGAGGAAATCATTAAAACTCAATTAAAAACAAGGAGCTACGTATCATCTGCAACAGTTGTTAATCTGGAGGAAGAGGGTTCAAGTTTGGACCAGGATGCCGATTACTGTATCATCTGCCAGGACCACTATCAGAACCGGGAGAAAGTTGGAATGCTCAATTGTGGACATGAGTACCACGCGAGTTGCTTAAAGAAATGGCTGCTCGTCAAGAATGTCTGTCCCGTCTGCAAATCGGAAGCATTGGCGACGGATAGAAAGGAACGA

Coding sequence (CDS)

ATGAGGCAGAGAATACCGCGCACTAGTCAGATGGTCGATATGGAAATGGATCGACAAGGTCAGAATTATCTTCATGCTGAGCCTTCCATCATTATGCCAGGCCCATCTAACTTCCCTCAGCATAGTATTCATTCAATGGTAACAGCTTCTGGGAATCCCGAGGCCCATTATCTACCCGATCCTTATGATGGCTCCATGTTACATGGTTTAAATCAGTATGGTAGCGTTCAGCATCATCACAGTCTAGGTTTGAGTACTGCAGCCCCTGGAAACTATTATTACTCTTACGTTACACCGCCATCAAGTAATGGGATGTTACCTGCACCTCTAAATCATAACGTGTCCGAGCAGTTGCCATCTTCTAGCAATTATGGAGTCATTCAAACGTCTTCTGATGGTTATGGAAGGAATTCATATTTTGTGGATGAAGTTAGTGATCCGCGTAAGAGAAAAATAACAGAAGGGATTCCTGGGAACATTCAACACATGAATGGTGTGGCCAGCTCTAGTTCTTCTGTGCATTTGTCGAATTCAAGGCATCCTGACGAGGTTGCTATGGTGGAAGCATCATCTTTTCCCCTGCCTCAGAGTAGATGGAATGGACCGCGGAGTAGTGTTAGAGCTGCATCAGGTGGAACTAGGCGGGACTCTCTTCTACCACCCGAACACAATCATTCAACTCTAGGAAATAATAGGGGTCAACACCTTCAGCCCGCTAATTCTAGTTTCTGGTTGGACCAACACTTACAGGCTAATTGTGGAAACGGAAGTGCTTCATCTTGGAACCAGACGTCTACCCCACCTTTCATGCACGGAACTAATACAAATGGAGGTTTGTTGGAAACCATGAATTTGGGTGTGCATAGATATCATGAAACGGCGGGGAACCGGAGTTCTAGAAATGTCCAACATCCTTCAGTTAACCATGGCCACCACATTCATAATCATCCATCTGCAGTTGTGCAAAGAATTAGAGGCCACAATTTCCAGTTCTACCCCCAAATGACAGCAGCTTCATATGGATTTCCTTTGAATTCTTCATATGCCACCATGAATCCTCATAGTTTGGAGATAGGGCGTAGGCAACCAGGACCAGTTCCACCTACCGGTCACGGGCTGCACCGGCTTCCACGAGCGAATGTTGCTGCTGACACTACAACTAGACATCATAATGTTCCTCAGTTGAGATTCTTGCAGACTGATGAAGTTGCATTATTGGAGATTCCGGAATTCTACGAAGTGGGGAATTTTGTTGACCATCACAGGGACATGCGATTGGATATAGAGGATATGTCTTACGAGGAACTTCTCGCTTTAGGGGAGCGAATTGGTAACGTCAGCACTGGGTTAACAGAGGAAATCATTAAAACTCAATTAAAAACAAGGAGCTACGTATCATCTGCAACAGTTGTTAATCTGGAGGAAGAGGGTTCAAGTTTGGACCAGGATGCCGATTACTGTATCATCTGCCAGGACCACTATCAGAACCGGGAGAAAGTTGGAATGCTCAATTGTGGACATGAGTACCACGCGAGTTGCTTAAAGAAATGGCTGCTCGTCAAGAATGTCTGTCCCGTCTGCAAATCGGAAGCATTGGCGACGGATAGAAAGGAACGA

Protein sequence

MRQRIPRTSQMVDMEMDRQGQNYLHAEPSIIMPGPSNFPQHSIHSMVTASGNPEAHYLPDPYDGSMLHGLNQYGSVQHHHSLGLSTAAPGNYYYSYVTPPSSNGMLPAPLNHNVSEQLPSSSNYGVIQTSSDGYGRNSYFVDEVSDPRKRKITEGIPGNIQHMNGVASSSSSVHLSNSRHPDEVAMVEASSFPLPQSRWNGPRSSVRAASGGTRRDSLLPPEHNHSTLGNNRGQHLQPANSSFWLDQHLQANCGNGSASSWNQTSTPPFMHGTNTNGGLLETMNLGVHRYHETAGNRSSRNVQHPSVNHGHHIHNHPSAVVQRIRGHNFQFYPQMTAASYGFPLNSSYATMNPHSLEIGRRQPGPVPPTGHGLHRLPRANVAADTTTRHHNVPQLRFLQTDEVALLEIPEFYEVGNFVDHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSYVSSATVVNLEEEGSSLDQDADYCIICQDHYQNREKVGMLNCGHEYHASCLKKWLLVKNVCPVCKSEALATDRKER
Homology
BLAST of MS002753 vs. NCBI nr
Match: XP_022142806.1 (probable E3 ubiquitin-protein ligase ZFP1 [Momordica charantia] >XP_022142807.1 probable E3 ubiquitin-protein ligase ZFP1 [Momordica charantia])

HSP 1 Score: 1096.6 bits (2835), Expect = 0.0e+00
Identity = 537/539 (99.63%), Postives = 537/539 (99.63%), Query Frame = 0

Query: 1   MRQRIPRTSQMVDMEMDRQGQNYLHAEPSIIMPGPSNFPQHSIHSMVTASGNPEAHYLPD 60
           MRQRIPRTSQMVDMEMDRQGQNYLHAEPSIIMPGPSNFPQHSIHSMVTASGNPEAHYLPD
Sbjct: 1   MRQRIPRTSQMVDMEMDRQGQNYLHAEPSIIMPGPSNFPQHSIHSMVTASGNPEAHYLPD 60

Query: 61  PYDGSMLHGLNQYGSVQHHHSLGLSTAAPGNYYYSYVTPPSSNGMLPAPLNHNVSEQLPS 120
           PYDGSMLHGLNQYGSVQHHHSLGLSTAAPGNYYYSYVTPPSSNGMLPAPLNHNVSEQLPS
Sbjct: 61  PYDGSMLHGLNQYGSVQHHHSLGLSTAAPGNYYYSYVTPPSSNGMLPAPLNHNVSEQLPS 120

Query: 121 SSNYGVIQTSSDGYGRNSYFVDEVSDPRKRKITEGIPGNIQHMNGVASSSSSVHLSNSRH 180
           SSNYGVIQTSSDGYGRNSYFVDEVSDPRKRKITEGIPGNIQHMNGVASSSSSVHLSNSRH
Sbjct: 121 SSNYGVIQTSSDGYGRNSYFVDEVSDPRKRKITEGIPGNIQHMNGVASSSSSVHLSNSRH 180

Query: 181 PDEVAMVEASSFPLPQSRWNGPRSSVRAASGGTRRDSLLPPEHNHSTLGNNRGQHLQPAN 240
           PDEVAMVEASSFPLPQSRWNGPRSSVRAASGGTRRDSLLPPEHNHSTLGNNRGQHLQPAN
Sbjct: 181 PDEVAMVEASSFPLPQSRWNGPRSSVRAASGGTRRDSLLPPEHNHSTLGNNRGQHLQPAN 240

Query: 241 SSFWLDQHLQANCGNGSASSWNQTSTPPFMHGTNTNGGLLETMNLGVHRYHETAGNRSSR 300
           SSFWLDQHLQANCGNGSASSWNQTSTPPFMHGTNTNGGLLETMNLGVHRYHETAGNRSSR
Sbjct: 241 SSFWLDQHLQANCGNGSASSWNQTSTPPFMHGTNTNGGLLETMNLGVHRYHETAGNRSSR 300

Query: 301 NVQHPSVNHGHHIHNHPSAVVQRIRGHNFQFYPQMTAASYGFPLNSSYATMNPHSLEIGR 360
           NVQHPSVNHGHHIHNHPSAVVQRIRGHNFQFYPQMTAASYGFPLNSSYATMNPHSLEIGR
Sbjct: 301 NVQHPSVNHGHHIHNHPSAVVQRIRGHNFQFYPQMTAASYGFPLNSSYATMNPHSLEIGR 360

Query: 361 RQPGPVPPTGHGLHRLPRANVAADTTTRHHNVPQLRFLQTDEVALLEIPEFYEVGNFVDH 420
           RQPGPVPPTGHGLH LPRANVAADTTTRHHNVPQLRFLQTDEVALLEIPEFYEVGNFVDH
Sbjct: 361 RQPGPVPPTGHGLHWLPRANVAADTTTRHHNVPQLRFLQTDEVALLEIPEFYEVGNFVDH 420

Query: 421 HRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSYVSSATVVNLEEEGSSL 480
           HRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSYVSSATVVNLEEEGSSL
Sbjct: 421 HRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSYVSSATVVNLEEEGSSL 480

Query: 481 DQDADYCIICQDHYQNREKVGMLNCGHEYHASCLKKWLLVKNVCPVCKSEALATDRKER 540
           DQDADYCIICQDHYQNREKVG LNCGHEYHASCLKKWLLVKNVCPVCKSEALATDRKER
Sbjct: 481 DQDADYCIICQDHYQNREKVGTLNCGHEYHASCLKKWLLVKNVCPVCKSEALATDRKER 539

BLAST of MS002753 vs. NCBI nr
Match: XP_038891302.1 (probable E3 ubiquitin-protein ligase ZFP1 [Benincasa hispida] >XP_038891310.1 probable E3 ubiquitin-protein ligase ZFP1 [Benincasa hispida] >XP_038891317.1 probable E3 ubiquitin-protein ligase ZFP1 [Benincasa hispida] >XP_038891325.1 probable E3 ubiquitin-protein ligase ZFP1 [Benincasa hispida] >XP_038891332.1 probable E3 ubiquitin-protein ligase ZFP1 [Benincasa hispida] >XP_038891340.1 probable E3 ubiquitin-protein ligase ZFP1 [Benincasa hispida])

HSP 1 Score: 1007.3 bits (2603), Expect = 5.0e-290
Identity = 486/543 (89.50%), Postives = 519/543 (95.58%), Query Frame = 0

Query: 1   MRQRIPRTSQMVDMEMDRQGQNYLHAEPSIIMPGPSNFPQHSIHSMVTASG---NPEAHY 60
           MRQRIPRTSQMVD+EMDRQGQNY+HAEPS+I+PG SNFPQ S+HSMVTASG   NPEAHY
Sbjct: 1   MRQRIPRTSQMVDLEMDRQGQNYIHAEPSVILPGTSNFPQQSMHSMVTASGNAPNPEAHY 60

Query: 61  LPDPYDGSMLHGLNQYGSVQHHHSLGLSTAAPGNYYYSYVTPPSSNGMLPAPLNHNVSEQ 120
           LPDPYDGSMLHGLNQY SVQHHHSLGL+TAAPGNYYYSY+TPPSSNG+LPAPLNHNV++Q
Sbjct: 61  LPDPYDGSMLHGLNQYSSVQHHHSLGLNTAAPGNYYYSYITPPSSNGLLPAPLNHNVADQ 120

Query: 121 LPSSSNYGVIQTSSDGYGRNSYFVDEVSDPRKRKITEGIPGNIQHMNGVASSSSSVHLSN 180
           LPSSSNYGVIQ SSDGYGRN+YF+DE+SDPRKRKITEGIPGN+Q +NG+AS+SSSVHLSN
Sbjct: 121 LPSSSNYGVIQNSSDGYGRNTYFLDEISDPRKRKITEGIPGNVQQLNGLASTSSSVHLSN 180

Query: 181 SRHPDEVAMVEASSFPLPQSRWNGPRSSVRAASGGTRRDSLLPPEHNHSTLGNNRGQHLQ 240
           SR PDEV MVEASSFP PQSRW+GPR+SVR+ S GTRRDSLLPP+HNHSTLGNNRGQHLQ
Sbjct: 181 SRLPDEVLMVEASSFPPPQSRWSGPRTSVRSGSSGTRRDSLLPPDHNHSTLGNNRGQHLQ 240

Query: 241 PANSSFWLDQHLQANCGNGSASSWNQTSTPPFMHGTNTNGGLLETMNLGVHRYHETAGNR 300
           PANS+FWLDQHLQANCGNGSASSWNQTST PFMHGTNTNGGLLETMNLGVHRYHETAGNR
Sbjct: 241 PANSTFWLDQHLQANCGNGSASSWNQTSTAPFMHGTNTNGGLLETMNLGVHRYHETAGNR 300

Query: 301 SSRNVQHPSVNHGHHIHNHPSAVVQRIRGHNFQFYPQMTAASYGFPLNSSYATMNPHSLE 360
           ++RN+QHPSVNHGHHIHNHPSAVVQRIRGHNFQFYPQ+TAASYGFPLNSSY TMNPHSLE
Sbjct: 301 NARNIQHPSVNHGHHIHNHPSAVVQRIRGHNFQFYPQVTAASYGFPLNSSYGTMNPHSLE 360

Query: 361 IGRRQPGPVPPTGHGLHRLPRANVAADTTTRHHNVPQLRFLQTDEVALLEIPEFYEVGNF 420
           IGRRQ G V PTGHGLHR+PRA+VAADTTTRHHN+PQLRFLQ DEVALLEIP+ YEVGN 
Sbjct: 361 IGRRQTGAVAPTGHGLHRIPRASVAADTTTRHHNIPQLRFLQADEVALLEIPDLYEVGNL 420

Query: 421 VDHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSYVSSATVVNL-EEE 480
           VDHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSYV+SATVVNL EEE
Sbjct: 421 VDHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSYVASATVVNLEEEE 480

Query: 481 GSSLDQDADYCIICQDHYQNREKVGMLNCGHEYHASCLKKWLLVKNVCPVCKSEALATDR 540
           GSSLDQDADYCIICQDHYQNREKVG LNCGHEYHASCLKKWLLVKNVCP+CKSEAL TDR
Sbjct: 481 GSSLDQDADYCIICQDHYQNREKVGTLNCGHEYHASCLKKWLLVKNVCPICKSEALVTDR 540

BLAST of MS002753 vs. NCBI nr
Match: XP_004137212.1 (probable E3 ubiquitin-protein ligase ZFP1 [Cucumis sativus] >XP_011653315.1 probable E3 ubiquitin-protein ligase ZFP1 [Cucumis sativus] >XP_011653318.1 probable E3 ubiquitin-protein ligase ZFP1 [Cucumis sativus] >XP_031740012.1 probable E3 ubiquitin-protein ligase ZFP1 [Cucumis sativus] >XP_031740013.1 probable E3 ubiquitin-protein ligase ZFP1 [Cucumis sativus] >XP_031740014.1 probable E3 ubiquitin-protein ligase ZFP1 [Cucumis sativus] >KGN53605.1 hypothetical protein Csa_015055 [Cucumis sativus])

HSP 1 Score: 991.1 bits (2561), Expect = 3.7e-285
Identity = 480/546 (87.91%), Postives = 514/546 (94.14%), Query Frame = 0

Query: 1   MRQRIPRTSQMVDMEMDRQGQNYLHAEPSIIMPGPSNFPQHSIHSMVTASG---NPEAHY 60
           MRQR+PRTSQMVD+EMDRQGQNYLHAEPS+I+PG SNFPQH + SMVTASG   NPE HY
Sbjct: 1   MRQRMPRTSQMVDLEMDRQGQNYLHAEPSVILPGTSNFPQHGMQSMVTASGNAPNPETHY 60

Query: 61  LPDPYDGSMLHGLNQYGSVQHHHSLGLSTAAPGNYYYSYVTPPSSNGMLPAPLNHNVSEQ 120
           LPDPYD SMLHGLNQYGSVQHHHSLGLSTAAPGNYYYSY+TPPSSNG+LPAPLNHNV++Q
Sbjct: 61  LPDPYDVSMLHGLNQYGSVQHHHSLGLSTAAPGNYYYSYITPPSSNGLLPAPLNHNVTDQ 120

Query: 121 LPSSSNYGVIQTSSDGYGRNSYFVDEVSDPRKRKITEGIPGNIQHMNGVASSSSSVHLSN 180
           LPSSSNYG IQTSS+GYGRN+YFVDE+SDPRKRKITEGIPGN+QH+NG+AS+SSS+HLSN
Sbjct: 121 LPSSSNYG-IQTSSNGYGRNTYFVDEISDPRKRKITEGIPGNVQHLNGLASTSSSMHLSN 180

Query: 181 SRHPDEVAMVEASSFPLPQSRWNGPRSSVRAASGGTRRDSLLPPEHNHSTLGNNRGQHLQ 240
           SR PDEVAMV ASSFP PQSRW+GPR+S RA S GTRRDS+LPP+HNHST+GNNRGQHLQ
Sbjct: 181 SRLPDEVAMVGASSFPPPQSRWSGPRNSARAGSSGTRRDSILPPDHNHSTIGNNRGQHLQ 240

Query: 241 PANSSFWLDQHLQANCGNGSASSWNQTSTPPFMHGTNTNGGLLETMNLGVHRYHETAGNR 300
           P NSSFWLDQHLQANCGNGSASSWNQTST PFMHGTNTNGGLLETMNLGVHRYHETAGNR
Sbjct: 241 PTNSSFWLDQHLQANCGNGSASSWNQTSTAPFMHGTNTNGGLLETMNLGVHRYHETAGNR 300

Query: 301 SSRNVQHPSVNHGHHIHNHPSAVVQRIRGHNFQFYPQMTAASYGFPLNSSYATMNPHSLE 360
           +SRN+QHPSVNHGHHIHNHPSAVVQRIRGHNFQFYPQ+TAASYGFPLNSSY TMNPHSLE
Sbjct: 301 NSRNIQHPSVNHGHHIHNHPSAVVQRIRGHNFQFYPQVTAASYGFPLNSSYGTMNPHSLE 360

Query: 361 IGRRQPGPVPPTGHGLHRLPRANVAADTTTRHHNVPQLRFLQTDEVALLEIPEFYEVGNF 420
           IGRRQPG V PTGHGLHR+PRA+VAADTTTRHH++PQLRFLQ DEVALLEIP+ YEVGN 
Sbjct: 361 IGRRQPGAVAPTGHGLHRIPRASVAADTTTRHHSIPQLRFLQADEVALLEIPDLYEVGNL 420

Query: 421 VDHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSYVSSATVVNL---- 480
           VDHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSY++S TVVNL    
Sbjct: 421 VDHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSYIASTTVVNLEEEE 480

Query: 481 EEEGSSLDQDADYCIICQDHYQNREKVGMLNCGHEYHASCLKKWLLVKNVCPVCKSEALA 540
           EEEGS+LDQD DYCIICQDHYQN EKVG L CGHEYHASCLKKWLLVKNVCP+CKSEALA
Sbjct: 481 EEEGSNLDQDVDYCIICQDHYQNLEKVGTLYCGHEYHASCLKKWLLVKNVCPICKSEALA 540

BLAST of MS002753 vs. NCBI nr
Match: XP_008451692.1 (PREDICTED: E3 ubiquitin-protein ligase Arkadia [Cucumis melo] >XP_008451694.1 PREDICTED: E3 ubiquitin-protein ligase Arkadia [Cucumis melo] >XP_008451696.1 PREDICTED: E3 ubiquitin-protein ligase Arkadia [Cucumis melo] >XP_008451697.1 PREDICTED: E3 ubiquitin-protein ligase Arkadia [Cucumis melo] >XP_008451699.1 PREDICTED: E3 ubiquitin-protein ligase Arkadia [Cucumis melo] >XP_016901177.1 PREDICTED: E3 ubiquitin-protein ligase Arkadia [Cucumis melo] >KAA0059006.1 E3 ubiquitin-protein ligase Arkadia [Cucumis melo var. makuwa] >TYK19562.1 E3 ubiquitin-protein ligase Arkadia [Cucumis melo var. makuwa])

HSP 1 Score: 988.0 bits (2553), Expect = 3.2e-284
Identity = 478/544 (87.87%), Postives = 513/544 (94.30%), Query Frame = 0

Query: 1   MRQRIPRTSQMVDMEMDRQGQNYLHAEPSIIMPGPSNFPQHSIHSMVTASG---NPEAHY 60
           MRQR+PRTSQMVD+EMDRQGQNYLHAEPS+I+PG SNFPQH + SMVTASG   NPE HY
Sbjct: 1   MRQRMPRTSQMVDLEMDRQGQNYLHAEPSVILPGTSNFPQHGMQSMVTASGNAPNPETHY 60

Query: 61  LPDPYDGSMLHGLNQYGSVQHHHSLGLSTAAPGNYYYSYVTPPSSNGMLPAPLNHNVSEQ 120
           LPDPYD SMLHGLNQY SVQHHHSLGLSTAAPGNYYYSY+TPPSSNG+LPAPLNHNV++Q
Sbjct: 61  LPDPYDVSMLHGLNQYSSVQHHHSLGLSTAAPGNYYYSYITPPSSNGLLPAPLNHNVNDQ 120

Query: 121 LPSSSNYGVIQTSSDGYGRNSYFVDEVSDPRKRKITEGIPGNIQHMNGVASSSSSVHLSN 180
           LPSSSNYG IQTSSDGYGRN+YFVDE+SDPRKRKITEGIPGN+QH++G+AS+SSS+HLSN
Sbjct: 121 LPSSSNYG-IQTSSDGYGRNTYFVDEISDPRKRKITEGIPGNVQHLSGLASTSSSMHLSN 180

Query: 181 SRHPDEVAMVEASSFPLPQSRWNGPRSSVRAASGGTRRDSLLPPEHNHSTLGNNRGQHLQ 240
           SR PDEVAMV ASSFP PQSRW+GPR+S RA S GTRRDS+LPP+HNHST+GNNRGQHLQ
Sbjct: 181 SRIPDEVAMVGASSFPPPQSRWSGPRNSARAGSSGTRRDSILPPDHNHSTIGNNRGQHLQ 240

Query: 241 PANSSFWLDQHLQANCGNGSASSWNQTSTPPFMHGTNTNGGLLETMNLGVHRYHETAGNR 300
           PANSSFWLDQHLQANCGNGSASSWNQ ST PFMHGTNTNGGLLETMNLGVHRYHETAGNR
Sbjct: 241 PANSSFWLDQHLQANCGNGSASSWNQNSTAPFMHGTNTNGGLLETMNLGVHRYHETAGNR 300

Query: 301 SSRNVQHPSVNHGHHIHNHPSAVVQRIRGHNFQFYPQMTAASYGFPLNSSYATMNPHSLE 360
           +SRN+QHPSVNHGHHIHNHPSAVVQRIRGHNFQFYPQ+TAASYGFPLNSSY TMNPHSLE
Sbjct: 301 NSRNIQHPSVNHGHHIHNHPSAVVQRIRGHNFQFYPQVTAASYGFPLNSSYGTMNPHSLE 360

Query: 361 IGRRQPGPVPPTGHGLHRLPRANVAADTTTRHHNVPQLRFLQTDEVALLEIPEFYEVGNF 420
           IGRRQPG V PTGHGLHR+PRA+VAADTTTRHH++PQLRFLQ DEVALLEIP+ YEVGN 
Sbjct: 361 IGRRQPGAVAPTGHGLHRIPRASVAADTTTRHHSIPQLRFLQADEVALLEIPDLYEVGNL 420

Query: 421 VDHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSYVSSATVVNL--EE 480
           VDHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEE IKTQLKTRSY++S TVVNL  EE
Sbjct: 421 VDHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEESIKTQLKTRSYIASTTVVNLEEEE 480

Query: 481 EGSSLDQDADYCIICQDHYQNREKVGMLNCGHEYHASCLKKWLLVKNVCPVCKSEALATD 540
           EGS+LDQD DYCIICQDHYQN EKVG L+CGHEYHASCLKKWLLVKNVCP+CKSEALATD
Sbjct: 481 EGSNLDQDVDYCIICQDHYQNLEKVGTLDCGHEYHASCLKKWLLVKNVCPICKSEALATD 540

BLAST of MS002753 vs. NCBI nr
Match: KAG7015036.1 (putative E3 ubiquitin-protein ligase ZFP1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 974.9 bits (2519), Expect = 2.8e-280
Identity = 476/548 (86.86%), Postives = 505/548 (92.15%), Query Frame = 0

Query: 1   MRQRIPRTSQMVDMEMDRQGQNYLHAEPSIIMPGPSNFPQHSIHSMVTASG---NPEAHY 60
           MRQRIP TS MVD+EMDR GQNYLHAEPS+I+PG SN PQHS+HSMVTASG   NPEAHY
Sbjct: 1   MRQRIPHTSPMVDLEMDRLGQNYLHAEPSVILPGISNLPQHSMHSMVTASGNAPNPEAHY 60

Query: 61  LPDPYDGSMLHGLNQYGSVQHHHSLGLSTAAPGNYYYSYVTPPSSNGMLPAPLNHNVSEQ 120
           LPDPYDGSMLHGLNQY SVQHHHSLGLST APGNYYYSYVT PSSNG+LPAPLNHNV++ 
Sbjct: 61  LPDPYDGSMLHGLNQYSSVQHHHSLGLSTPAPGNYYYSYVTLPSSNGLLPAPLNHNVNDH 120

Query: 121 LPSSSNYGVIQTSSDGYGRNSYFVDEVSDPRKRKITEGIPGNIQHMNGVASSSSSVHLSN 180
           LPSSSNYGVIQTSSDGYGRNSY VDEVSDPRKRKITEGIPGN+QH+NG+ASSSSSVHLSN
Sbjct: 121 LPSSSNYGVIQTSSDGYGRNSYSVDEVSDPRKRKITEGIPGNVQHLNGLASSSSSVHLSN 180

Query: 181 SRHPDEVAMVEASSFPLPQSRWNGPRSSVRAASGGTRRDSLLPPEHNHSTLGNNRGQHLQ 240
           SRHPDEVAMVEASSFPLP SRW+GPRSSVR  S GTR D +LPP+HN STLGNNRGQHLQ
Sbjct: 181 SRHPDEVAMVEASSFPLPHSRWSGPRSSVRVGSSGTRHDPVLPPDHNLSTLGNNRGQHLQ 240

Query: 241 PANSSFWLDQHLQANCGNGSASSWNQTSTPPFMHGTNTNGGLLETMNLGVHRYHETAGNR 300
           PANS+FWLDQHLQANCGNGSASSWNQTST PFMHGTNTNGGLLETMN+GVHRYHETAGNR
Sbjct: 241 PANSTFWLDQHLQANCGNGSASSWNQTSTAPFMHGTNTNGGLLETMNVGVHRYHETAGNR 300

Query: 301 SSRNVQHPSVNHGHHIHNHPSAVVQRIRGHNFQFYPQMTAASYGFPLNSSYATMNPHSLE 360
           +SRN+QHPS+NHGHHIHNHPSAVVQRIRGHNFQ YPQ+TAA YGFPLNSSY TMNPH+LE
Sbjct: 301 NSRNIQHPSINHGHHIHNHPSAVVQRIRGHNFQSYPQVTAALYGFPLNSSYGTMNPHNLE 360

Query: 361 IGRRQPGPVPPTGHGLHRLPRANVAADTTTRHHNVPQLRFLQTDEVALLEIPEFYEVGNF 420
           IGRRQPG V PTGHGLHRLPRA VA DTTTR HN+ QLRFLQ DEVALLEIP+ YEVGN 
Sbjct: 361 IGRRQPGVVAPTGHGLHRLPRAGVAPDTTTRPHNISQLRFLQADEVALLEIPDLYEVGNS 420

Query: 421 VDHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSYVSSATVVNLE--- 480
           +DHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSYV+   VVNLE   
Sbjct: 421 IDHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSYVAPTIVVNLEEDE 480

Query: 481 ---EEGSSLDQDADYCIICQDHYQNREKVGMLNCGHEYHASCLKKWLLVKNVCPVCKSEA 540
              E+GSSLDQDADYCIICQDHY+NREKVG L+CGHEYHASCLKKWLLVKNVCP+CKSEA
Sbjct: 481 DEDEQGSSLDQDADYCIICQDHYKNREKVGTLDCGHEYHASCLKKWLLVKNVCPICKSEA 540

BLAST of MS002753 vs. ExPASy Swiss-Prot
Match: Q5QLR5 (Probable E3 ubiquitin-protein ligase ZFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=ZFP1 PE=1 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 2.9e-38
Identity = 162/552 (29.35%), Postives = 249/552 (45.11%), Query Frame = 0

Query: 8   TSQMVDMEMDRQGQNYLHAEPSIIMPGPSNFPQHSIHSMVTASGNPEAHYLPDP---YDG 67
           T Q++D+E + QG +++H+E S    G  +  Q + H+ V   GN     L D    YD 
Sbjct: 9   TPQVIDLETE-QGHSHIHSE-SFNRTGNDSSDQGAQHA-VRGVGNATNIGLSDMRSYYDA 68

Query: 68  SMLHGLNQYGSVQHHHSLGLSTAAPGNYYYSYVTPPSSNGMLPAPLNHNVSEQLPSSSNY 127
            M H      ++  +  +      P + Y   ++  S N      ++H  S  LPS+   
Sbjct: 69  GMNHPHQPVHNLPPNLGVDSGFVFPSSMYNPCMSTTSMNQY----VSHTQSFGLPSNQ-- 128

Query: 128 GVIQTSSDGYGRNSYFVDEVSDPRKRKITEGIPGNIQHMNGVASSSSSVHLSNSRHPDEV 187
            V+  S D   RN    +      KRK    + G+    NG ASSSSS    N  H    
Sbjct: 129 -VVLGSMDEGSRNENAGESARGFIKRK-NAAVAGSYHCANGFASSSSSHASLNPTHRPWD 188

Query: 188 AMVEASSFP----------LPQSRWNGPRSSVRAASG----GTRRDSLLPPEHNHSTLGN 247
              E++  P            Q+ W     S   ++G    G   +S      N++   +
Sbjct: 189 PSFESNVLPNTASYNPSEYHSQTSWPSMEGSSIPSNGFNLMGAHPES--AQHGNYAFPTS 248

Query: 248 NRGQHLQPANSSFWLDQHLQANCGNGSASSWNQTSTPPFMHGTNTNGGLLETMNLGVHRY 307
           +  Q  QP  S+ W+ Q   AN        W   +      G  +  G+ ET+N G  R 
Sbjct: 249 HISQCFQP-TSNTWISQ--SANGIADGIPQWEYVNGMNNAPGRFSRSGMTETVN-GSFRE 308

Query: 308 HETAGNRSSRNV-----QHPSVNHGHHIHNHPSAVVQRIRGHNFQFYPQMTAASYGFPLN 367
           ++   +   R       QH  + H H++ +H        + HN      +  +   F L 
Sbjct: 309 YQNGPSTLCRGPLPYFHQHAGM-HAHNLLDHTQVQAPYQQCHNNPVLHGVNHSGNRFHLG 368

Query: 368 SSYATMNPHSLEIGRRQPGPVPPTGHGLHRLPRANVAADTTTRHHNVPQLRFLQTDEVAL 427
                +  +S     R  GP           P   + A+    H N+   R L  +   +
Sbjct: 369 PRIPVLFSNS----ERTFGP-----------PHHPLLAN-PVNHRNI---RILPPEHATI 428

Query: 428 LEIPEFYEVGNFVDHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSYV 487
           ++    YEV N VD HRDMRLDI+ M+YEELLAL E+IG+V+TGL +  I  +LKT  +V
Sbjct: 429 MDFSRLYEVSNVVDEHRDMRLDIDSMTYEELLALEEQIGDVNTGLAKSYIVEKLKTSLFV 488

Query: 488 SSATVVNLEEEGSSLDQDADYCIICQDHYQNREKVGMLNCGHEYHASCLKKWLLVKNVCP 538
             ++ ++ +   SS++ DA  CIICQ+ YQ +E +G L+CGH YH  C+K+WL+VKN+CP
Sbjct: 489 PGSSCMSNKSSESSMENDA--CIICQEEYQVKECIGTLDCGHRYHEDCIKQWLMVKNLCP 521

BLAST of MS002753 vs. ExPASy Swiss-Prot
Match: O49500 (E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 6.4e-30
Identity = 60/116 (51.72%), Postives = 84/116 (72.41%), Query Frame = 0

Query: 419 DHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSYVSSATVVNLEEEGS 478
           D HRDMRLD+++MSYEELLALGERIG+VSTGL+EE+I   +K   + SSA         +
Sbjct: 560 DRHRDMRLDVDNMSYEELLALGERIGDVSTGLSEEVILKVMKQHKHTSSA---------A 619

Query: 479 SLDQDADYCIICQDHYQNREKVGMLNCGHEYHASCLKKWLLVKNVCPVCKSEALAT 535
              QD + C +CQ+ Y   + +G L CGHE+H +C+K+WL++KN+CP+CK+ AL+T
Sbjct: 620 GSHQDMEPCCVCQEEYAEGDDLGTLGCGHEFHTACVKQWLMLKNLCPICKTVALST 666

BLAST of MS002753 vs. ExPASy Swiss-Prot
Match: Q9ZQF9 (E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 1.1e-29
Identity = 59/116 (50.86%), Postives = 83/116 (71.55%), Query Frame = 0

Query: 419 DHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSYVSSATVVNLEEEGS 478
           D HR+MRLD+++MSYEELLALGERIG+VSTGL+EE+I   +K   + SS+          
Sbjct: 594 DRHREMRLDVDNMSYEELLALGERIGDVSTGLSEEVILKAMKQHKHTSSS------PSSV 653

Query: 479 SLDQDADYCIICQDHYQNREKVGMLNCGHEYHASCLKKWLLVKNVCPVCKSEALAT 535
            L Q+ + C ICQ+ Y   + +G L CGHE+H  C+K+W+++KN+CP+CK+EAL T
Sbjct: 654 ELHQNIEPCCICQEEYVEGDNLGTLKCGHEFHKDCIKQWVMIKNLCPICKTEALKT 703

BLAST of MS002753 vs. ExPASy Swiss-Prot
Match: Q9FMM4 (Probable E3 ubiquitin-protein ligase RHG1A OS=Arabidopsis thaliana OX=3702 GN=RHG1A PE=2 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 1.1e-29
Identity = 64/142 (45.07%), Postives = 90/142 (63.38%), Query Frame = 0

Query: 398 LQTDEVALLEIPEFYEVGNFVDHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKT 457
           L+ ++V LL     ++     D +RDMRLD+++MSYEELLAL ERIG+V TG+ EE I  
Sbjct: 558 LRLEDVMLLNHSVLFDGATGHDRYRDMRLDVDNMSYEELLALEERIGDVCTGVNEETISN 617

Query: 458 QLKTRSYVSSATVVNLEEEGSSLDQDADYCIICQDHYQNREKVGMLNCGHEYHASCLKKW 517
           +LK R Y S+          +   QDA+ C +CQ+ Y   E +G L CGHE+H+ C+K+W
Sbjct: 618 RLKQRKYKSN----------TKSPQDAEPCCVCQEEYTEGEDMGTLECGHEFHSQCIKEW 677

Query: 518 LLVKNVCPVCKSEALATDRKER 540
           L  KN+CP+CK+  L T +K R
Sbjct: 678 LKQKNLCPICKTTGLNTAKKRR 689

BLAST of MS002753 vs. ExPASy Swiss-Prot
Match: Q7XTV7 (Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=39947 GN=HIP1 PE=1 SV=2)

HSP 1 Score: 128.3 bits (321), Expect = 2.7e-28
Identity = 62/122 (50.82%), Postives = 83/122 (68.03%), Query Frame = 0

Query: 411 FYEVGNFVDHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSYVSSATV 470
           FY   +  D HRDMRLDI++MSYEELLAL ERIGNVSTGL+EE +   LK R + S    
Sbjct: 553 FYGGVDIHDRHRDMRLDIDNMSYEELLALEERIGNVSTGLSEEEVTKLLKQRKFSSWRLE 612

Query: 471 VNLEEEGSSLDQDADYCIICQDHYQNREKVGMLNCGHEYHASCLKKWLLVKNVCPVCKSE 530
            ++EEE          C ICQ+ Y + + +G L+CGH++H  C+++WL+VKN CP+CK+ 
Sbjct: 613 ASVEEEP---------CCICQEEYVDGDDLGTLDCGHDFHVGCVRQWLVVKNTCPICKNT 665

Query: 531 AL 533
           AL
Sbjct: 673 AL 665

BLAST of MS002753 vs. ExPASy TrEMBL
Match: A0A6J1CNX4 (probable E3 ubiquitin-protein ligase ZFP1 OS=Momordica charantia OX=3673 GN=LOC111012840 PE=4 SV=1)

HSP 1 Score: 1096.6 bits (2835), Expect = 0.0e+00
Identity = 537/539 (99.63%), Postives = 537/539 (99.63%), Query Frame = 0

Query: 1   MRQRIPRTSQMVDMEMDRQGQNYLHAEPSIIMPGPSNFPQHSIHSMVTASGNPEAHYLPD 60
           MRQRIPRTSQMVDMEMDRQGQNYLHAEPSIIMPGPSNFPQHSIHSMVTASGNPEAHYLPD
Sbjct: 1   MRQRIPRTSQMVDMEMDRQGQNYLHAEPSIIMPGPSNFPQHSIHSMVTASGNPEAHYLPD 60

Query: 61  PYDGSMLHGLNQYGSVQHHHSLGLSTAAPGNYYYSYVTPPSSNGMLPAPLNHNVSEQLPS 120
           PYDGSMLHGLNQYGSVQHHHSLGLSTAAPGNYYYSYVTPPSSNGMLPAPLNHNVSEQLPS
Sbjct: 61  PYDGSMLHGLNQYGSVQHHHSLGLSTAAPGNYYYSYVTPPSSNGMLPAPLNHNVSEQLPS 120

Query: 121 SSNYGVIQTSSDGYGRNSYFVDEVSDPRKRKITEGIPGNIQHMNGVASSSSSVHLSNSRH 180
           SSNYGVIQTSSDGYGRNSYFVDEVSDPRKRKITEGIPGNIQHMNGVASSSSSVHLSNSRH
Sbjct: 121 SSNYGVIQTSSDGYGRNSYFVDEVSDPRKRKITEGIPGNIQHMNGVASSSSSVHLSNSRH 180

Query: 181 PDEVAMVEASSFPLPQSRWNGPRSSVRAASGGTRRDSLLPPEHNHSTLGNNRGQHLQPAN 240
           PDEVAMVEASSFPLPQSRWNGPRSSVRAASGGTRRDSLLPPEHNHSTLGNNRGQHLQPAN
Sbjct: 181 PDEVAMVEASSFPLPQSRWNGPRSSVRAASGGTRRDSLLPPEHNHSTLGNNRGQHLQPAN 240

Query: 241 SSFWLDQHLQANCGNGSASSWNQTSTPPFMHGTNTNGGLLETMNLGVHRYHETAGNRSSR 300
           SSFWLDQHLQANCGNGSASSWNQTSTPPFMHGTNTNGGLLETMNLGVHRYHETAGNRSSR
Sbjct: 241 SSFWLDQHLQANCGNGSASSWNQTSTPPFMHGTNTNGGLLETMNLGVHRYHETAGNRSSR 300

Query: 301 NVQHPSVNHGHHIHNHPSAVVQRIRGHNFQFYPQMTAASYGFPLNSSYATMNPHSLEIGR 360
           NVQHPSVNHGHHIHNHPSAVVQRIRGHNFQFYPQMTAASYGFPLNSSYATMNPHSLEIGR
Sbjct: 301 NVQHPSVNHGHHIHNHPSAVVQRIRGHNFQFYPQMTAASYGFPLNSSYATMNPHSLEIGR 360

Query: 361 RQPGPVPPTGHGLHRLPRANVAADTTTRHHNVPQLRFLQTDEVALLEIPEFYEVGNFVDH 420
           RQPGPVPPTGHGLH LPRANVAADTTTRHHNVPQLRFLQTDEVALLEIPEFYEVGNFVDH
Sbjct: 361 RQPGPVPPTGHGLHWLPRANVAADTTTRHHNVPQLRFLQTDEVALLEIPEFYEVGNFVDH 420

Query: 421 HRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSYVSSATVVNLEEEGSSL 480
           HRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSYVSSATVVNLEEEGSSL
Sbjct: 421 HRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSYVSSATVVNLEEEGSSL 480

Query: 481 DQDADYCIICQDHYQNREKVGMLNCGHEYHASCLKKWLLVKNVCPVCKSEALATDRKER 540
           DQDADYCIICQDHYQNREKVG LNCGHEYHASCLKKWLLVKNVCPVCKSEALATDRKER
Sbjct: 481 DQDADYCIICQDHYQNREKVGTLNCGHEYHASCLKKWLLVKNVCPVCKSEALATDRKER 539

BLAST of MS002753 vs. ExPASy TrEMBL
Match: A0A0A0KVI3 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G089270 PE=4 SV=1)

HSP 1 Score: 991.1 bits (2561), Expect = 1.8e-285
Identity = 480/546 (87.91%), Postives = 514/546 (94.14%), Query Frame = 0

Query: 1   MRQRIPRTSQMVDMEMDRQGQNYLHAEPSIIMPGPSNFPQHSIHSMVTASG---NPEAHY 60
           MRQR+PRTSQMVD+EMDRQGQNYLHAEPS+I+PG SNFPQH + SMVTASG   NPE HY
Sbjct: 1   MRQRMPRTSQMVDLEMDRQGQNYLHAEPSVILPGTSNFPQHGMQSMVTASGNAPNPETHY 60

Query: 61  LPDPYDGSMLHGLNQYGSVQHHHSLGLSTAAPGNYYYSYVTPPSSNGMLPAPLNHNVSEQ 120
           LPDPYD SMLHGLNQYGSVQHHHSLGLSTAAPGNYYYSY+TPPSSNG+LPAPLNHNV++Q
Sbjct: 61  LPDPYDVSMLHGLNQYGSVQHHHSLGLSTAAPGNYYYSYITPPSSNGLLPAPLNHNVTDQ 120

Query: 121 LPSSSNYGVIQTSSDGYGRNSYFVDEVSDPRKRKITEGIPGNIQHMNGVASSSSSVHLSN 180
           LPSSSNYG IQTSS+GYGRN+YFVDE+SDPRKRKITEGIPGN+QH+NG+AS+SSS+HLSN
Sbjct: 121 LPSSSNYG-IQTSSNGYGRNTYFVDEISDPRKRKITEGIPGNVQHLNGLASTSSSMHLSN 180

Query: 181 SRHPDEVAMVEASSFPLPQSRWNGPRSSVRAASGGTRRDSLLPPEHNHSTLGNNRGQHLQ 240
           SR PDEVAMV ASSFP PQSRW+GPR+S RA S GTRRDS+LPP+HNHST+GNNRGQHLQ
Sbjct: 181 SRLPDEVAMVGASSFPPPQSRWSGPRNSARAGSSGTRRDSILPPDHNHSTIGNNRGQHLQ 240

Query: 241 PANSSFWLDQHLQANCGNGSASSWNQTSTPPFMHGTNTNGGLLETMNLGVHRYHETAGNR 300
           P NSSFWLDQHLQANCGNGSASSWNQTST PFMHGTNTNGGLLETMNLGVHRYHETAGNR
Sbjct: 241 PTNSSFWLDQHLQANCGNGSASSWNQTSTAPFMHGTNTNGGLLETMNLGVHRYHETAGNR 300

Query: 301 SSRNVQHPSVNHGHHIHNHPSAVVQRIRGHNFQFYPQMTAASYGFPLNSSYATMNPHSLE 360
           +SRN+QHPSVNHGHHIHNHPSAVVQRIRGHNFQFYPQ+TAASYGFPLNSSY TMNPHSLE
Sbjct: 301 NSRNIQHPSVNHGHHIHNHPSAVVQRIRGHNFQFYPQVTAASYGFPLNSSYGTMNPHSLE 360

Query: 361 IGRRQPGPVPPTGHGLHRLPRANVAADTTTRHHNVPQLRFLQTDEVALLEIPEFYEVGNF 420
           IGRRQPG V PTGHGLHR+PRA+VAADTTTRHH++PQLRFLQ DEVALLEIP+ YEVGN 
Sbjct: 361 IGRRQPGAVAPTGHGLHRIPRASVAADTTTRHHSIPQLRFLQADEVALLEIPDLYEVGNL 420

Query: 421 VDHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSYVSSATVVNL---- 480
           VDHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSY++S TVVNL    
Sbjct: 421 VDHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSYIASTTVVNLEEEE 480

Query: 481 EEEGSSLDQDADYCIICQDHYQNREKVGMLNCGHEYHASCLKKWLLVKNVCPVCKSEALA 540
           EEEGS+LDQD DYCIICQDHYQN EKVG L CGHEYHASCLKKWLLVKNVCP+CKSEALA
Sbjct: 481 EEEGSNLDQDVDYCIICQDHYQNLEKVGTLYCGHEYHASCLKKWLLVKNVCPICKSEALA 540

BLAST of MS002753 vs. ExPASy TrEMBL
Match: A0A1S3BRI1 (E3 ubiquitin-protein ligase Arkadia OS=Cucumis melo OX=3656 GN=LOC103492915 PE=4 SV=1)

HSP 1 Score: 988.0 bits (2553), Expect = 1.5e-284
Identity = 478/544 (87.87%), Postives = 513/544 (94.30%), Query Frame = 0

Query: 1   MRQRIPRTSQMVDMEMDRQGQNYLHAEPSIIMPGPSNFPQHSIHSMVTASG---NPEAHY 60
           MRQR+PRTSQMVD+EMDRQGQNYLHAEPS+I+PG SNFPQH + SMVTASG   NPE HY
Sbjct: 1   MRQRMPRTSQMVDLEMDRQGQNYLHAEPSVILPGTSNFPQHGMQSMVTASGNAPNPETHY 60

Query: 61  LPDPYDGSMLHGLNQYGSVQHHHSLGLSTAAPGNYYYSYVTPPSSNGMLPAPLNHNVSEQ 120
           LPDPYD SMLHGLNQY SVQHHHSLGLSTAAPGNYYYSY+TPPSSNG+LPAPLNHNV++Q
Sbjct: 61  LPDPYDVSMLHGLNQYSSVQHHHSLGLSTAAPGNYYYSYITPPSSNGLLPAPLNHNVNDQ 120

Query: 121 LPSSSNYGVIQTSSDGYGRNSYFVDEVSDPRKRKITEGIPGNIQHMNGVASSSSSVHLSN 180
           LPSSSNYG IQTSSDGYGRN+YFVDE+SDPRKRKITEGIPGN+QH++G+AS+SSS+HLSN
Sbjct: 121 LPSSSNYG-IQTSSDGYGRNTYFVDEISDPRKRKITEGIPGNVQHLSGLASTSSSMHLSN 180

Query: 181 SRHPDEVAMVEASSFPLPQSRWNGPRSSVRAASGGTRRDSLLPPEHNHSTLGNNRGQHLQ 240
           SR PDEVAMV ASSFP PQSRW+GPR+S RA S GTRRDS+LPP+HNHST+GNNRGQHLQ
Sbjct: 181 SRIPDEVAMVGASSFPPPQSRWSGPRNSARAGSSGTRRDSILPPDHNHSTIGNNRGQHLQ 240

Query: 241 PANSSFWLDQHLQANCGNGSASSWNQTSTPPFMHGTNTNGGLLETMNLGVHRYHETAGNR 300
           PANSSFWLDQHLQANCGNGSASSWNQ ST PFMHGTNTNGGLLETMNLGVHRYHETAGNR
Sbjct: 241 PANSSFWLDQHLQANCGNGSASSWNQNSTAPFMHGTNTNGGLLETMNLGVHRYHETAGNR 300

Query: 301 SSRNVQHPSVNHGHHIHNHPSAVVQRIRGHNFQFYPQMTAASYGFPLNSSYATMNPHSLE 360
           +SRN+QHPSVNHGHHIHNHPSAVVQRIRGHNFQFYPQ+TAASYGFPLNSSY TMNPHSLE
Sbjct: 301 NSRNIQHPSVNHGHHIHNHPSAVVQRIRGHNFQFYPQVTAASYGFPLNSSYGTMNPHSLE 360

Query: 361 IGRRQPGPVPPTGHGLHRLPRANVAADTTTRHHNVPQLRFLQTDEVALLEIPEFYEVGNF 420
           IGRRQPG V PTGHGLHR+PRA+VAADTTTRHH++PQLRFLQ DEVALLEIP+ YEVGN 
Sbjct: 361 IGRRQPGAVAPTGHGLHRIPRASVAADTTTRHHSIPQLRFLQADEVALLEIPDLYEVGNL 420

Query: 421 VDHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSYVSSATVVNL--EE 480
           VDHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEE IKTQLKTRSY++S TVVNL  EE
Sbjct: 421 VDHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEESIKTQLKTRSYIASTTVVNLEEEE 480

Query: 481 EGSSLDQDADYCIICQDHYQNREKVGMLNCGHEYHASCLKKWLLVKNVCPVCKSEALATD 540
           EGS+LDQD DYCIICQDHYQN EKVG L+CGHEYHASCLKKWLLVKNVCP+CKSEALATD
Sbjct: 481 EGSNLDQDVDYCIICQDHYQNLEKVGTLDCGHEYHASCLKKWLLVKNVCPICKSEALATD 540

BLAST of MS002753 vs. ExPASy TrEMBL
Match: A0A5A7UXT9 (E3 ubiquitin-protein ligase Arkadia OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold416G00630 PE=4 SV=1)

HSP 1 Score: 988.0 bits (2553), Expect = 1.5e-284
Identity = 478/544 (87.87%), Postives = 513/544 (94.30%), Query Frame = 0

Query: 1   MRQRIPRTSQMVDMEMDRQGQNYLHAEPSIIMPGPSNFPQHSIHSMVTASG---NPEAHY 60
           MRQR+PRTSQMVD+EMDRQGQNYLHAEPS+I+PG SNFPQH + SMVTASG   NPE HY
Sbjct: 1   MRQRMPRTSQMVDLEMDRQGQNYLHAEPSVILPGTSNFPQHGMQSMVTASGNAPNPETHY 60

Query: 61  LPDPYDGSMLHGLNQYGSVQHHHSLGLSTAAPGNYYYSYVTPPSSNGMLPAPLNHNVSEQ 120
           LPDPYD SMLHGLNQY SVQHHHSLGLSTAAPGNYYYSY+TPPSSNG+LPAPLNHNV++Q
Sbjct: 61  LPDPYDVSMLHGLNQYSSVQHHHSLGLSTAAPGNYYYSYITPPSSNGLLPAPLNHNVNDQ 120

Query: 121 LPSSSNYGVIQTSSDGYGRNSYFVDEVSDPRKRKITEGIPGNIQHMNGVASSSSSVHLSN 180
           LPSSSNYG IQTSSDGYGRN+YFVDE+SDPRKRKITEGIPGN+QH++G+AS+SSS+HLSN
Sbjct: 121 LPSSSNYG-IQTSSDGYGRNTYFVDEISDPRKRKITEGIPGNVQHLSGLASTSSSMHLSN 180

Query: 181 SRHPDEVAMVEASSFPLPQSRWNGPRSSVRAASGGTRRDSLLPPEHNHSTLGNNRGQHLQ 240
           SR PDEVAMV ASSFP PQSRW+GPR+S RA S GTRRDS+LPP+HNHST+GNNRGQHLQ
Sbjct: 181 SRIPDEVAMVGASSFPPPQSRWSGPRNSARAGSSGTRRDSILPPDHNHSTIGNNRGQHLQ 240

Query: 241 PANSSFWLDQHLQANCGNGSASSWNQTSTPPFMHGTNTNGGLLETMNLGVHRYHETAGNR 300
           PANSSFWLDQHLQANCGNGSASSWNQ ST PFMHGTNTNGGLLETMNLGVHRYHETAGNR
Sbjct: 241 PANSSFWLDQHLQANCGNGSASSWNQNSTAPFMHGTNTNGGLLETMNLGVHRYHETAGNR 300

Query: 301 SSRNVQHPSVNHGHHIHNHPSAVVQRIRGHNFQFYPQMTAASYGFPLNSSYATMNPHSLE 360
           +SRN+QHPSVNHGHHIHNHPSAVVQRIRGHNFQFYPQ+TAASYGFPLNSSY TMNPHSLE
Sbjct: 301 NSRNIQHPSVNHGHHIHNHPSAVVQRIRGHNFQFYPQVTAASYGFPLNSSYGTMNPHSLE 360

Query: 361 IGRRQPGPVPPTGHGLHRLPRANVAADTTTRHHNVPQLRFLQTDEVALLEIPEFYEVGNF 420
           IGRRQPG V PTGHGLHR+PRA+VAADTTTRHH++PQLRFLQ DEVALLEIP+ YEVGN 
Sbjct: 361 IGRRQPGAVAPTGHGLHRIPRASVAADTTTRHHSIPQLRFLQADEVALLEIPDLYEVGNL 420

Query: 421 VDHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSYVSSATVVNL--EE 480
           VDHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEE IKTQLKTRSY++S TVVNL  EE
Sbjct: 421 VDHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEESIKTQLKTRSYIASTTVVNLEEEE 480

Query: 481 EGSSLDQDADYCIICQDHYQNREKVGMLNCGHEYHASCLKKWLLVKNVCPVCKSEALATD 540
           EGS+LDQD DYCIICQDHYQN EKVG L+CGHEYHASCLKKWLLVKNVCP+CKSEALATD
Sbjct: 481 EGSNLDQDVDYCIICQDHYQNLEKVGTLDCGHEYHASCLKKWLLVKNVCPICKSEALATD 540

BLAST of MS002753 vs. ExPASy TrEMBL
Match: A0A6J1E7E3 (probable E3 ubiquitin-protein ligase ZFP1 OS=Cucurbita moschata OX=3662 GN=LOC111430591 PE=4 SV=1)

HSP 1 Score: 974.2 bits (2517), Expect = 2.3e-280
Identity = 475/548 (86.68%), Postives = 505/548 (92.15%), Query Frame = 0

Query: 1   MRQRIPRTSQMVDMEMDRQGQNYLHAEPSIIMPGPSNFPQHSIHSMVTASG---NPEAHY 60
           MRQRIP TS MVD+EMDR GQNY HAEPS+I+PG SN PQHS+HS+VTASG   NPEAHY
Sbjct: 1   MRQRIPHTSPMVDLEMDRLGQNYFHAEPSVILPGISNLPQHSMHSLVTASGNAPNPEAHY 60

Query: 61  LPDPYDGSMLHGLNQYGSVQHHHSLGLSTAAPGNYYYSYVTPPSSNGMLPAPLNHNVSEQ 120
           LPDPYDGSMLHGLNQY SVQHHHSLGLST APGNYYYSYVT PSSNG+LPAPLNHNV++ 
Sbjct: 61  LPDPYDGSMLHGLNQYSSVQHHHSLGLSTPAPGNYYYSYVTLPSSNGLLPAPLNHNVNDH 120

Query: 121 LPSSSNYGVIQTSSDGYGRNSYFVDEVSDPRKRKITEGIPGNIQHMNGVASSSSSVHLSN 180
           LPSSSNYGVIQTSSDGYGRNSY VDEVSDPRKRKITEGIPGN+QH+NG+ASSSSSVHLSN
Sbjct: 121 LPSSSNYGVIQTSSDGYGRNSYSVDEVSDPRKRKITEGIPGNVQHLNGLASSSSSVHLSN 180

Query: 181 SRHPDEVAMVEASSFPLPQSRWNGPRSSVRAASGGTRRDSLLPPEHNHSTLGNNRGQHLQ 240
           SRHPDEVAMVEASSFPLPQSRW+GPRSSVR  S GTR D +LPP+HN STLGNNRGQHLQ
Sbjct: 181 SRHPDEVAMVEASSFPLPQSRWSGPRSSVRVGSSGTRHDPVLPPDHNLSTLGNNRGQHLQ 240

Query: 241 PANSSFWLDQHLQANCGNGSASSWNQTSTPPFMHGTNTNGGLLETMNLGVHRYHETAGNR 300
           PANS+FWLDQHLQANCGNGSASSWNQTST PFMHGTNTNGGLLETMN+GVHRYHETAGNR
Sbjct: 241 PANSTFWLDQHLQANCGNGSASSWNQTSTAPFMHGTNTNGGLLETMNVGVHRYHETAGNR 300

Query: 301 SSRNVQHPSVNHGHHIHNHPSAVVQRIRGHNFQFYPQMTAASYGFPLNSSYATMNPHSLE 360
           +SRN+QHPS+NHGHHIHNHPSAVVQRIRGHNFQ YPQ+TAA YGFPLNSSY TMNPH+LE
Sbjct: 301 NSRNIQHPSINHGHHIHNHPSAVVQRIRGHNFQSYPQVTAALYGFPLNSSYGTMNPHNLE 360

Query: 361 IGRRQPGPVPPTGHGLHRLPRANVAADTTTRHHNVPQLRFLQTDEVALLEIPEFYEVGNF 420
           IGRRQPG V PTGHGLHRLPRA VA DTTTR HN+ QLRFLQ DEVALLEIP+ YEVGN 
Sbjct: 361 IGRRQPGVVAPTGHGLHRLPRAGVAPDTTTRPHNISQLRFLQADEVALLEIPDLYEVGNS 420

Query: 421 VDHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSYVSSATVVNLE--- 480
           +DHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSYV+   VVNLE   
Sbjct: 421 IDHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSYVAPTIVVNLEEDE 480

Query: 481 ---EEGSSLDQDADYCIICQDHYQNREKVGMLNCGHEYHASCLKKWLLVKNVCPVCKSEA 540
              E+GSSLDQDADYCIICQDHY+NREKVG L+CGHEYHASCLKKWLLVKNVCP+CKSEA
Sbjct: 481 DEDEQGSSLDQDADYCIICQDHYKNREKVGTLDCGHEYHASCLKKWLLVKNVCPICKSEA 540

BLAST of MS002753 vs. TAIR 10
Match: AT1G53190.1 (RING/U-box superfamily protein )

HSP 1 Score: 279.3 bits (713), Expect = 6.7e-75
Identity = 214/554 (38.63%), Postives = 291/554 (52.53%), Query Frame = 0

Query: 12  VDMEMD-RQGQNYLHAEPSIIMPGPSNFPQ----HSIHSMVTASGNPEAHYLPDP-YDGS 71
           VD+EM+ +Q Q  L AEP I++    +FPQ    +++ +MV    N E H L DP YD S
Sbjct: 8   VDLEMEQQQSQASLQAEPCILL---GSFPQQPDNNNMPAMVAHVPNLEPHSLQDPTYDNS 67

Query: 72  -MLHGLNQYGSVQHHHSLGLSTAAPGNYYYSYVTPPSSNGMLPAPLNHNVSEQLPSSSNY 131
            M +GL QY    HHH        P N+Y  YV   +  G            QLPSSS++
Sbjct: 68  AMFYGLPQY----HHHP---HQRVPTNFYVPYVAFQAPPG------------QLPSSSSH 127

Query: 132 GVIQTSSD-GYGRNSYFVDEVSDPRKRKITEGIPGNIQHMNGVASSSSSVHLSNSRHPDE 191
           GV+  S D  Y RN++F+D      KRK  EGIPG  Q+++ +A+  ++        P+ 
Sbjct: 128 GVVGVSPDHEYERNAHFMDHTRGTYKRKNAEGIPGQPQYLSTLAAPFNT--------PET 187

Query: 192 VAMVEASSFPLPQSRWNGPRSSVRAASGGTRRDSLLPPE-HNHSTLGNNRGQHLQPANSS 251
           +A                P    R   G    +++LP    N+   GN  G H  P   S
Sbjct: 188 IA----------------PFGGARNRPGAVTVNTVLPSHAPNNFIQGNYAGHHPFPPPGS 247

Query: 252 FWLDQHLQANCGNGSASSWNQTSTPPFMHGTNTNGGLLETMNLGVHRYHETAGNRSSRNV 311
            W DQH      +GS S W      P+MHG+N   G +E            + +R+  + 
Sbjct: 248 MWYDQH--HGRSDGSPSFW----PTPYMHGSNIFAGSIE------------SSSRNPTSF 307

Query: 312 QHPSV----NHGHHIHNHPS-AVVQRIRGHNFQFYPQ-MTAASYGFPLNS--SYATMN-- 371
            +PS     +H +  H+HP+   VQ +RG N   YP   ++ASY  P  S     TMN  
Sbjct: 308 MYPSQLNPRDHYYSHHHHPAPPPVQGMRGQNATLYPHTASSASYRVPPGSFTPQNTMNSG 367

Query: 372 PHSLEIGRRQPGPVPPTGHGL--HRLPRANVAADTTTRHH-NVPQLRFLQTDEVALLEIP 431
           P   E+G    GPV PTG  +  H     +V   T  +H   VP+LR +  DEVALLE  
Sbjct: 368 PLGSEMGSSHMGPVQPTGFRIYQHHQRDDSVPVATLRQHRGGVPRLRVMPDDEVALLEFG 427

Query: 432 EFY--EVGNFVDHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSYVSS 491
           +F      N +DHHRDMRLDIE+MSYEELLAL ERIG V+TGL EE +K  LKTR    +
Sbjct: 428 DFLGGSGNNHIDHHRDMRLDIEEMSYEELLALSERIGTVNTGLPEEDVKNHLKTR----T 487

Query: 492 ATVVNLEEEGSS---LDQDADYCIICQDHYQNREKVGMLNCGHEYHASCLKKWLLVKNVC 539
            + +N E+E SS    D + + C ICQ+ ++N EK+  L+CGHEYHA CL+KWL+VKNVC
Sbjct: 488 CSGINFEKESSSPRTKDLETEPCTICQESFKNEEKIATLDCGHEYHAECLEKWLIVKNVC 493

BLAST of MS002753 vs. TAIR 10
Match: AT1G53190.2 (RING/U-box superfamily protein )

HSP 1 Score: 279.3 bits (713), Expect = 6.7e-75
Identity = 214/554 (38.63%), Postives = 291/554 (52.53%), Query Frame = 0

Query: 12  VDMEMD-RQGQNYLHAEPSIIMPGPSNFPQ----HSIHSMVTASGNPEAHYLPDP-YDGS 71
           VD+EM+ +Q Q  L AEP I++    +FPQ    +++ +MV    N E H L DP YD S
Sbjct: 8   VDLEMEQQQSQASLQAEPCILL---GSFPQQPDNNNMPAMVAHVPNLEPHSLQDPTYDNS 67

Query: 72  -MLHGLNQYGSVQHHHSLGLSTAAPGNYYYSYVTPPSSNGMLPAPLNHNVSEQLPSSSNY 131
            M +GL QY    HHH        P N+Y  YV   +  G            QLPSSS++
Sbjct: 68  AMFYGLPQY----HHHP---HQRVPTNFYVPYVAFQAPPG------------QLPSSSSH 127

Query: 132 GVIQTSSD-GYGRNSYFVDEVSDPRKRKITEGIPGNIQHMNGVASSSSSVHLSNSRHPDE 191
           GV+  S D  Y RN++F+D      KRK  EGIPG  Q+++ +A+  ++        P+ 
Sbjct: 128 GVVGVSPDHEYERNAHFMDHTRGTYKRKNAEGIPGQPQYLSTLAAPFNT--------PET 187

Query: 192 VAMVEASSFPLPQSRWNGPRSSVRAASGGTRRDSLLPPE-HNHSTLGNNRGQHLQPANSS 251
           +A                P    R   G    +++LP    N+   GN  G H  P   S
Sbjct: 188 IA----------------PFGGARNRPGAVTVNTVLPSHAPNNFIQGNYAGHHPFPPPGS 247

Query: 252 FWLDQHLQANCGNGSASSWNQTSTPPFMHGTNTNGGLLETMNLGVHRYHETAGNRSSRNV 311
            W DQH      +GS S W      P+MHG+N   G +E            + +R+  + 
Sbjct: 248 MWYDQH--HGRSDGSPSFW----PTPYMHGSNIFAGSIE------------SSSRNPTSF 307

Query: 312 QHPSV----NHGHHIHNHPS-AVVQRIRGHNFQFYPQ-MTAASYGFPLNS--SYATMN-- 371
            +PS     +H +  H+HP+   VQ +RG N   YP   ++ASY  P  S     TMN  
Sbjct: 308 MYPSQLNPRDHYYSHHHHPAPPPVQGMRGQNATLYPHTASSASYRVPPGSFTPQNTMNSG 367

Query: 372 PHSLEIGRRQPGPVPPTGHGL--HRLPRANVAADTTTRHH-NVPQLRFLQTDEVALLEIP 431
           P   E+G    GPV PTG  +  H     +V   T  +H   VP+LR +  DEVALLE  
Sbjct: 368 PLGSEMGSSHMGPVQPTGFRIYQHHQRDDSVPVATLRQHRGGVPRLRVMPDDEVALLEFG 427

Query: 432 EFY--EVGNFVDHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSYVSS 491
           +F      N +DHHRDMRLDIE+MSYEELLAL ERIG V+TGL EE +K  LKTR    +
Sbjct: 428 DFLGGSGNNHIDHHRDMRLDIEEMSYEELLALSERIGTVNTGLPEEDVKNHLKTR----T 487

Query: 492 ATVVNLEEEGSS---LDQDADYCIICQDHYQNREKVGMLNCGHEYHASCLKKWLLVKNVC 539
            + +N E+E SS    D + + C ICQ+ ++N EK+  L+CGHEYHA CL+KWL+VKNVC
Sbjct: 488 CSGINFEKESSSPRTKDLETEPCTICQESFKNEEKIATLDCGHEYHAECLEKWLIVKNVC 493

BLAST of MS002753 vs. TAIR 10
Match: AT3G15070.1 (RING/U-box superfamily protein )

HSP 1 Score: 273.9 bits (699), Expect = 2.8e-73
Identity = 199/542 (36.72%), Postives = 281/542 (51.85%), Query Frame = 0

Query: 1   MRQRIPRTSQMVDMEMDRQGQNYL-HAEPSIIMPGPSNFPQHSIHSMVTASGNPEAHYLP 60
           MRQR    + M   +M++  Q+++ H EP I     +N+    I  + T   + EAH L 
Sbjct: 1   MRQR----NMMTGSDMEQNSQSFVPHPEPRI----GTNYLYPDIPPVNTVP-HLEAHSLQ 60

Query: 61  DPYD-GSMLHGLNQYGSVQHHHSLGLSTAAPGNYYYSYVTPPSSNGMLPAPLNHNVSEQL 120
           +PYD  SM +G  QY   QH  +LG   +   N+Y  YV   +    L           L
Sbjct: 61  EPYDNNSMFYGPPQYHH-QHASNLGSGMSTAPNFYVPYVNYEAPPSFL-----------L 120

Query: 121 PSSSNYGVIQTSSDGYGRNSYFVDEVSDPRKRKITEGIPGNIQHMNGVASSSSSVHLSNS 180
              SN  V+  +S  + RN++F+D      KRK +E IPGN Q+      +  S    N+
Sbjct: 121 SHGSNDAVVGVTSTEHERNAHFMDH---GFKRKSSEVIPGNSQY----PVAPCSFPQLNT 180

Query: 181 RHPDEVAMVEASSFPLPQSRWNGPRSSVRAASGGTRRDSLLPPEHNHSTLGNNRGQHLQP 240
                 +     ++P P       + SVR  +G    D LL   HN+ + G N   H  P
Sbjct: 181 SETAPFSFPHFGTYPQPLD-----QRSVRNRAGAATMDPLLSHGHNNFSQG-NYAAHPFP 240

Query: 241 ANSSFWLDQHLQANCGNGSASSWNQTSTPPFMHGTNTNGGLLETMNLGVHRYHETAGNRS 300
              S W DQH   N  +GS+S W+Q    P+MHG N   G +E+ N+   RYHET+   S
Sbjct: 241 PLGSIWYDQHCNGNRSDGSSSLWSQAPAVPYMHG-NIATGSIESGNVCFPRYHETS---S 300

Query: 301 SRNVQHPSVNHGHHIHNHPSAVVQRIRGHNFQFYPQMTAASYGFPLN-SSYATMNPHSLE 360
           SRN         H+I +HP      +       YP M +ASY   L+ +SY+ M      
Sbjct: 301 SRNPTPSVYQRNHYISHHPVPPPPIV-------YPHMPSASYAETLHPASYSHM------ 360

Query: 361 IGRRQPGPVPPTGHGLHRLPRANVAADTTTRHHNVPQLRFLQTDEVALLEIPEFYEVGNF 420
                 G V  TG  +++ P  +       RH  +P  R +  +E A  E+ +FY   N+
Sbjct: 361 ------GQVQSTGFRINQYPGEDFVPAAILRHRELPHFRAMPANENAFWEVGDFYNAVNY 420

Query: 421 VDHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSYVSSATVVNLEEEG 480
           VDHH+DMRLDIEDMSYEELLAL ++IG V TGL+ E +K  LK R    ++T +NL EEG
Sbjct: 421 VDHHQDMRLDIEDMSYEELLALSDQIGTVKTGLSSEDVKELLKRR----TSTRINL-EEG 480

Query: 481 SSLDQDADYCIICQDHYQNREKVGMLNCGHEYHASCLKKWLLVKNVCPVCKSEALATDRK 540
            S D + D C ICQ++Y+N +K+  L+C H+YHA CLKKWL++KNVCP+CKSEAL  ++K
Sbjct: 481 PSTDLETDSCTICQENYKNEDKIATLDCMHKYHAECLKKWLVIKNVCPICKSEALVIEKK 480

BLAST of MS002753 vs. TAIR 10
Match: AT3G15070.2 (RING/U-box superfamily protein )

HSP 1 Score: 273.9 bits (699), Expect = 2.8e-73
Identity = 199/542 (36.72%), Postives = 281/542 (51.85%), Query Frame = 0

Query: 1   MRQRIPRTSQMVDMEMDRQGQNYL-HAEPSIIMPGPSNFPQHSIHSMVTASGNPEAHYLP 60
           MRQR    + M   +M++  Q+++ H EP I     +N+    I  + T   + EAH L 
Sbjct: 1   MRQR----NMMTGSDMEQNSQSFVPHPEPRI----GTNYLYPDIPPVNTVP-HLEAHSLQ 60

Query: 61  DPYD-GSMLHGLNQYGSVQHHHSLGLSTAAPGNYYYSYVTPPSSNGMLPAPLNHNVSEQL 120
           +PYD  SM +G  QY   QH  +LG   +   N+Y  YV   +    L           L
Sbjct: 61  EPYDNNSMFYGPPQYHH-QHASNLGSGMSTAPNFYVPYVNYEAPPSFL-----------L 120

Query: 121 PSSSNYGVIQTSSDGYGRNSYFVDEVSDPRKRKITEGIPGNIQHMNGVASSSSSVHLSNS 180
              SN  V+  +S  + RN++F+D      KRK +E IPGN Q+      +  S    N+
Sbjct: 121 SHGSNDAVVGVTSTEHERNAHFMDH---GFKRKSSEVIPGNSQY----PVAPCSFPQLNT 180

Query: 181 RHPDEVAMVEASSFPLPQSRWNGPRSSVRAASGGTRRDSLLPPEHNHSTLGNNRGQHLQP 240
                 +     ++P P       + SVR  +G    D LL   HN+ + G N   H  P
Sbjct: 181 SETAPFSFPHFGTYPQPLD-----QRSVRNRAGAATMDPLLSHGHNNFSQG-NYAAHPFP 240

Query: 241 ANSSFWLDQHLQANCGNGSASSWNQTSTPPFMHGTNTNGGLLETMNLGVHRYHETAGNRS 300
              S W DQH   N  +GS+S W+Q    P+MHG N   G +E+ N+   RYHET+   S
Sbjct: 241 PLGSIWYDQHCNGNRSDGSSSLWSQAPAVPYMHG-NIATGSIESGNVCFPRYHETS---S 300

Query: 301 SRNVQHPSVNHGHHIHNHPSAVVQRIRGHNFQFYPQMTAASYGFPLN-SSYATMNPHSLE 360
           SRN         H+I +HP      +       YP M +ASY   L+ +SY+ M      
Sbjct: 301 SRNPTPSVYQRNHYISHHPVPPPPIV-------YPHMPSASYAETLHPASYSHM------ 360

Query: 361 IGRRQPGPVPPTGHGLHRLPRANVAADTTTRHHNVPQLRFLQTDEVALLEIPEFYEVGNF 420
                 G V  TG  +++ P  +       RH  +P  R +  +E A  E+ +FY   N+
Sbjct: 361 ------GQVQSTGFRINQYPGEDFVPAAILRHRELPHFRAMPANENAFWEVGDFYNAVNY 420

Query: 421 VDHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSYVSSATVVNLEEEG 480
           VDHH+DMRLDIEDMSYEELLAL ++IG V TGL+ E +K  LK R    ++T +NL EEG
Sbjct: 421 VDHHQDMRLDIEDMSYEELLALSDQIGTVKTGLSSEDVKELLKRR----TSTRINL-EEG 480

Query: 481 SSLDQDADYCIICQDHYQNREKVGMLNCGHEYHASCLKKWLLVKNVCPVCKSEALATDRK 540
            S D + D C ICQ++Y+N +K+  L+C H+YHA CLKKWL++KNVCP+CKSEAL  ++K
Sbjct: 481 PSTDLETDSCTICQENYKNEDKIATLDCMHKYHAECLKKWLVIKNVCPICKSEALVIEKK 480

BLAST of MS002753 vs. TAIR 10
Match: AT4G34040.1 (RING/U-box superfamily protein )

HSP 1 Score: 133.7 bits (335), Expect = 4.6e-31
Identity = 60/116 (51.72%), Postives = 84/116 (72.41%), Query Frame = 0

Query: 419 DHHRDMRLDIEDMSYEELLALGERIGNVSTGLTEEIIKTQLKTRSYVSSATVVNLEEEGS 478
           D HRDMRLD+++MSYEELLALGERIG+VSTGL+EE+I   +K   + SSA         +
Sbjct: 560 DRHRDMRLDVDNMSYEELLALGERIGDVSTGLSEEVILKVMKQHKHTSSA---------A 619

Query: 479 SLDQDADYCIICQDHYQNREKVGMLNCGHEYHASCLKKWLLVKNVCPVCKSEALAT 535
              QD + C +CQ+ Y   + +G L CGHE+H +C+K+WL++KN+CP+CK+ AL+T
Sbjct: 620 GSHQDMEPCCVCQEEYAEGDDLGTLGCGHEFHTACVKQWLMLKNLCPICKTVALST 666

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022142806.10.0e+0099.63probable E3 ubiquitin-protein ligase ZFP1 [Momordica charantia] >XP_022142807.1 ... [more]
XP_038891302.15.0e-29089.50probable E3 ubiquitin-protein ligase ZFP1 [Benincasa hispida] >XP_038891310.1 pr... [more]
XP_004137212.13.7e-28587.91probable E3 ubiquitin-protein ligase ZFP1 [Cucumis sativus] >XP_011653315.1 prob... [more]
XP_008451692.13.2e-28487.87PREDICTED: E3 ubiquitin-protein ligase Arkadia [Cucumis melo] >XP_008451694.1 PR... [more]
KAG7015036.12.8e-28086.86putative E3 ubiquitin-protein ligase ZFP1, partial [Cucurbita argyrosperma subsp... [more]
Match NameE-valueIdentityDescription
Q5QLR52.9e-3829.35Probable E3 ubiquitin-protein ligase ZFP1 OS=Oryza sativa subsp. japonica OX=399... [more]
O495006.4e-3051.72E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV... [more]
Q9ZQF91.1e-2950.86E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV... [more]
Q9FMM41.1e-2945.07Probable E3 ubiquitin-protein ligase RHG1A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Q7XTV72.7e-2850.82Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=399... [more]
Match NameE-valueIdentityDescription
A0A6J1CNX40.0e+0099.63probable E3 ubiquitin-protein ligase ZFP1 OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A0A0KVI31.8e-28587.91RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G089270 P... [more]
A0A1S3BRI11.5e-28487.87E3 ubiquitin-protein ligase Arkadia OS=Cucumis melo OX=3656 GN=LOC103492915 PE=4... [more]
A0A5A7UXT91.5e-28487.87E3 ubiquitin-protein ligase Arkadia OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A6J1E7E32.3e-28086.68probable E3 ubiquitin-protein ligase ZFP1 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT1G53190.16.7e-7538.63RING/U-box superfamily protein [more]
AT1G53190.26.7e-7538.63RING/U-box superfamily protein [more]
AT3G15070.12.8e-7336.72RING/U-box superfamily protein [more]
AT3G15070.22.8e-7336.72RING/U-box superfamily protein [more]
AT4G34040.14.6e-3151.72RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 487..527
e-value: 1.5E-6
score: 37.7
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 486..528
e-value: 5.2E-10
score: 39.5
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 487..528
score: 12.238477
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 464..533
e-value: 4.1E-17
score: 63.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 195..211
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 195..235
NoneNo IPR availablePANTHERPTHR22937:SF130SUBFAMILY NOT NAMEDcoord: 8..535
NoneNo IPR availablePANTHERPTHR22937E3 UBIQUITIN-PROTEIN LIGASE RNF165coord: 8..535
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 486..531

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS002753.1MS002753.1mRNA