MS002456 (gene) Bitter gourd (TR) v1

Overview
NameMS002456
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionGTD-binding domain-containing protein
Locationscaffold318: 171628 .. 173930 (-)
RNA-Seq ExpressionMS002456
SyntenyMS002456
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCACTCCACGAGATCCATCCATGGACCCTCTCCGGATTACTCGGAGCCTTTCTCGATCTTGCTCTCGTTTATGTTCTCCTCTGCATATCCGCCACTGCCTTCATCCCCTGCAAGATTCTCAAGATGATTGGATTGTTCCTGCCTTGCCCCTGCAGTGGGTTCTACGGCCGTCAGAACGCTAATCTATGCTTCCGCAGACTGCTCTTCAATTGGCCCAAGAGGAAGATCTCTTCCGTCCTCCACTTCGTCAAGACCAGGTTTCCTTTCGATTTGCTTTTGGTCGACGACCAAATTGGTAACTCCAATAGGAAGCTTCAGACCAAGGGGGTTCCTCAATCGCAGCAGAATCTCGCTGATAAAGATGGCGACTATGATAGAAAGGGCAAGAGGGTTATGTACCAGAGGCCCAGGACTAAAATCCGGCGTGGGAGGAGAGCTGCTGTTGAATGTGGGAGATTGGCCAGGGGAATCTGTGACAGCAATGAAGCTAGGAAGGAAAGGGAATCCGTGGCGTTGGTTGAGAGAGATGAATTTATTCACGGTGCGTATGTAATCTTATCCTATCACTCTCCTCTGTTTTCCCCTTTTCTTTATATTTCGTCAATTATTTATTTTTCTCTATATGTTATGACTTGTATGATATAAGCTTCTCTTTTTACGGATATACGGACATACGGACACGGCAAAGAAACATTTAATCAAATATAAATGCTTTCTGGAAGGAAATTCGCATTTGTATGTTGAGTCTGTATGATGTAATTTACTTCCAAGATTTATTATTTTTTATTCTGACCTATTTCTATGTCTTATGGCTTATGCTTAGCAAGTGTGTGTCTAATAAGTGTTGAATTCATTTTCACTTTGTACCACTAGTTTGTGACACAAATCTATCAAGTGTCCGATACATGTTCAATAAGTGTGGGAGTGTCCGACATGAACATGTCACCCGCGTCCGTGCTTCTTAGGTTCTGTTGCATGGGGATATGATATATTTGTCTATTTGTTTTCCTGGTTATGAACATTTCTTTGAAGTTTAGTAGAACTGTTGCTAACTGATTTGATGCCAGCCTATCAAAGAATTTTTGTTTGTATTTCAGCTCTGATAAAAACATTTATCGAAGTAAGTCGGAAGATACATTGTCTGATTTCTTTTTCCCTTAATTGTTATGACTATGATACAAGTTCACTGTTCATCTTCATATAGAGAGGTAGTTGTGCTAGCAAAGAAGAACTAACCGGCTAATCAAAAAAGTTACCAACATTAGCTAATATGCATAAACGTTAAATTTTTTGGAAGTTGTTCTTATTTAAATGCGAATCCCTGTCTGCTTTGTTTTTATTTTGTTACAGGCGGGTCAAGGGAGGGGTTTTACATCAAAATTTCGTTATTACCATGCTCATGTTTCTGTGTCCTTGCTATTTCAGATGTTATTAATGAATCAAATCACATTCACTTGAGCGAAAGAACCTGGCAAGGTTCCGAATCAAGTGGTTCGGTTGGCGAAAATAATTATATAGATAAAGGTTCTTCAACTATAGGACAAGATACCAGCAATGCCGAAGAGAGAGGCATTATCAGAAATGAAGCAAGCTCTATTGGATTGTTGGAGGAAGCGCTTGAAGAAGAGAAAGCTGCTCGGGCATCTCTGTATATGGAACTGGAGGAGGAGAGAGCAGCTGCTGCTACTGCTGCTGACGAAGCAATAGCCATGATAATACGTTTACAAAATGAGAAGGCATCAGTTGAAATGGAAGCAAGACAATATCAGAGGGTAATAGAAGAAAAATTTGCTTACGATGAAGAAGAGATGAATCTACTTAGAGAGATCCTTGTCAAGAGGGAAATAGATTATCACGTACTGGAGAAGGAAATTGAAGCATATAGGGAGATGGATTTTTCAGAAGAGGAACAATCAATAAGAAATTGGGATTTTATATTGGATGACCATAAAGAACAGTCTGCCACTACCCATTACTCAAATGGAGATCCACCCATTGTTCAGCAAATTGAGAATGCTATTTCTCTTTCAAGGAAAGCAAAGTTGAATGAAACTAAAAGTTATAACTCCCAATGCCATTTTAATGAAGGCGGCTTGCTTGAGCAAACTACCTGGACTAATGAAGACAATGAACTGAAGGATAATAGCTTAGTATGTGAGCATTTCACTTCTAAGGCAGCTCATGTGGTTCGCAGTTTAGGAAGTTCCGAACTTGATATGGAAATAGATGTTCAAGATATTCATGTGATTGATGAAAAACTCCACCTAGGAGACAAGAAGGAGGATATAAAAGTTGATAAT

mRNA sequence

ATGTCACTCCACGAGATCCATCCATGGACCCTCTCCGGATTACTCGGAGCCTTTCTCGATCTTGCTCTCGTTTATGTTCTCCTCTGCATATCCGCCACTGCCTTCATCCCCTGCAAGATTCTCAAGATGATTGGATTGTTCCTGCCTTGCCCCTGCAGTGGGTTCTACGGCCGTCAGAACGCTAATCTATGCTTCCGCAGACTGCTCTTCAATTGGCCCAAGAGGAAGATCTCTTCCGTCCTCCACTTCGTCAAGACCAGGTTTCCTTTCGATTTGCTTTTGGTCGACGACCAAATTGGTAACTCCAATAGGAAGCTTCAGACCAAGGGGGTTCCTCAATCGCAGCAGAATCTCGCTGATAAAGATGGCGACTATGATAGAAAGGGCAAGAGGGTTATGTACCAGAGGCCCAGGACTAAAATCCGGCGTGGGAGGAGAGCTGCTGTTGAATGTGGGAGATTGGCCAGGGGAATCTGTGACAGCAATGAAGCTAGGAAGGAAAGGGAATCCGTGGCGTTGGTTGAGAGAGATGAATTTATTCACGATGTTATTAATGAATCAAATCACATTCACTTGAGCGAAAGAACCTGGCAAGGTTCCGAATCAAGTGGTTCGGTTGGCGAAAATAATTATATAGATAAAGGTTCTTCAACTATAGGACAAGATACCAGCAATGCCGAAGAGAGAGGCATTATCAGAAATGAAGCAAGCTCTATTGGATTGTTGGAGGAAGCGCTTGAAGAAGAGAAAGCTGCTCGGGCATCTCTGTATATGGAACTGGAGGAGGAGAGAGCAGCTGCTGCTACTGCTGCTGACGAAGCAATAGCCATGATAATACGTTTACAAAATGAGAAGGCATCAGTTGAAATGGAAGCAAGACAATATCAGAGGGTAATAGAAGAAAAATTTGCTTACGATGAAGAAGAGATGAATCTACTTAGAGAGATCCTTGTCAAGAGGGAAATAGATTATCACGTACTGGAGAAGGAAATTGAAGCATATAGGGAGATGGATTTTTCAGAAGAGGAACAATCAATAAGAAATTGGGATTTTATATTGGATGACCATAAAGAACAGTCTGCCACTACCCATTACTCAAATGGAGATCCACCCATTGTTCAGCAAATTGAGAATGCTATTTCTCTTTCAAGGAAAGCAAAGTTGAATGAAACTAAAAGTTATAACTCCCAATGCCATTTTAATGAAGGCGGCTTGCTTGAGCAAACTACCTGGACTAATGAAGACAATGAACTGAAGGATAATAGCTTAGTATGTGAGCATTTCACTTCTAAGGCAGCTCATGTGGTTCGCAGTTTAGGAAGTTCCGAACTTGATATGGAAATAGATGTTCAAGATATTCATGTGATTGATGAAAAACTCCACCTAGGAGACAAGAAGGAGGATATAAAAGTTGATAAT

Coding sequence (CDS)

ATGTCACTCCACGAGATCCATCCATGGACCCTCTCCGGATTACTCGGAGCCTTTCTCGATCTTGCTCTCGTTTATGTTCTCCTCTGCATATCCGCCACTGCCTTCATCCCCTGCAAGATTCTCAAGATGATTGGATTGTTCCTGCCTTGCCCCTGCAGTGGGTTCTACGGCCGTCAGAACGCTAATCTATGCTTCCGCAGACTGCTCTTCAATTGGCCCAAGAGGAAGATCTCTTCCGTCCTCCACTTCGTCAAGACCAGGTTTCCTTTCGATTTGCTTTTGGTCGACGACCAAATTGGTAACTCCAATAGGAAGCTTCAGACCAAGGGGGTTCCTCAATCGCAGCAGAATCTCGCTGATAAAGATGGCGACTATGATAGAAAGGGCAAGAGGGTTATGTACCAGAGGCCCAGGACTAAAATCCGGCGTGGGAGGAGAGCTGCTGTTGAATGTGGGAGATTGGCCAGGGGAATCTGTGACAGCAATGAAGCTAGGAAGGAAAGGGAATCCGTGGCGTTGGTTGAGAGAGATGAATTTATTCACGATGTTATTAATGAATCAAATCACATTCACTTGAGCGAAAGAACCTGGCAAGGTTCCGAATCAAGTGGTTCGGTTGGCGAAAATAATTATATAGATAAAGGTTCTTCAACTATAGGACAAGATACCAGCAATGCCGAAGAGAGAGGCATTATCAGAAATGAAGCAAGCTCTATTGGATTGTTGGAGGAAGCGCTTGAAGAAGAGAAAGCTGCTCGGGCATCTCTGTATATGGAACTGGAGGAGGAGAGAGCAGCTGCTGCTACTGCTGCTGACGAAGCAATAGCCATGATAATACGTTTACAAAATGAGAAGGCATCAGTTGAAATGGAAGCAAGACAATATCAGAGGGTAATAGAAGAAAAATTTGCTTACGATGAAGAAGAGATGAATCTACTTAGAGAGATCCTTGTCAAGAGGGAAATAGATTATCACGTACTGGAGAAGGAAATTGAAGCATATAGGGAGATGGATTTTTCAGAAGAGGAACAATCAATAAGAAATTGGGATTTTATATTGGATGACCATAAAGAACAGTCTGCCACTACCCATTACTCAAATGGAGATCCACCCATTGTTCAGCAAATTGAGAATGCTATTTCTCTTTCAAGGAAAGCAAAGTTGAATGAAACTAAAAGTTATAACTCCCAATGCCATTTTAATGAAGGCGGCTTGCTTGAGCAAACTACCTGGACTAATGAAGACAATGAACTGAAGGATAATAGCTTAGTATGTGAGCATTTCACTTCTAAGGCAGCTCATGTGGTTCGCAGTTTAGGAAGTTCCGAACTTGATATGGAAATAGATGTTCAAGATATTCATGTGATTGATGAAAAACTCCACCTAGGAGACAAGAAGGAGGATATAAAAGTTGATAAT

Protein sequence

MSLHEIHPWTLSGLLGAFLDLALVYVLLCISATAFIPCKILKMIGLFLPCPCSGFYGRQNANLCFRRLLFNWPKRKISSVLHFVKTRFPFDLLLVDDQIGNSNRKLQTKGVPQSQQNLADKDGDYDRKGKRVMYQRPRTKIRRGRRAAVECGRLARGICDSNEARKERESVALVERDEFIHDVINESNHIHLSERTWQGSESSGSVGENNYIDKGSSTIGQDTSNAEERGIIRNEASSIGLLEEALEEEKAARASLYMELEEERAAAATAADEAIAMIIRLQNEKASVEMEARQYQRVIEEKFAYDEEEMNLLREILVKREIDYHVLEKEIEAYREMDFSEEEQSIRNWDFILDDHKEQSATTHYSNGDPPIVQQIENAISLSRKAKLNETKSYNSQCHFNEGGLLEQTTWTNEDNELKDNSLVCEHFTSKAAHVVRSLGSSELDMEIDVQDIHVIDEKLHLGDKKEDIKVDN
Homology
BLAST of MS002456 vs. NCBI nr
Match: XP_022150079.1 (uncharacterized protein LOC111018343 [Momordica charantia])

HSP 1 Score: 906.4 bits (2341), Expect = 1.1e-259
Identity = 470/473 (99.37%), Postives = 472/473 (99.79%), Query Frame = 0

Query: 1   MSLHEIHPWTLSGLLGAFLDLALVYVLLCISATAFIPCKILKMIGLFLPCPCSGFYGRQN 60
           MSLHEIHPWTLSGLLGAFLDLALVYVLLCISATAFIPCKILKMIGLFLPCPCSGFYGRQN
Sbjct: 1   MSLHEIHPWTLSGLLGAFLDLALVYVLLCISATAFIPCKILKMIGLFLPCPCSGFYGRQN 60

Query: 61  ANLCFRRLLFNWPKRKISSVLHFVKTRFPFDLLLVDDQIGNSNRKLQTKGVPQSQQNLAD 120
           ANLCFRRLLFNWPKRKISSVLHFVKTRFPFDLLLVDDQIGNSNRKLQTKGVPQSQQNLAD
Sbjct: 61  ANLCFRRLLFNWPKRKISSVLHFVKTRFPFDLLLVDDQIGNSNRKLQTKGVPQSQQNLAD 120

Query: 121 KDGDYDRKGKRVMYQRPRTKIRRGRRAAVECGRLARGICDSNEARKERESVALVERDEFI 180
           +DGDYDRKGKRVMYQRPRTKIRRGRRAAVECGRLARGICDSNEARKERESVALVERDEFI
Sbjct: 121 RDGDYDRKGKRVMYQRPRTKIRRGRRAAVECGRLARGICDSNEARKERESVALVERDEFI 180

Query: 181 HDVINESNHIHLSERTWQGSESSGSVGENNYIDKGSSTIGQDTSNAEERGIIRNEASSIG 240
           HDVINESNHIHLSERTWQGSESSGSVGENNYIDKGSSTIGQDTSNAEERGIIRNEASSIG
Sbjct: 181 HDVINESNHIHLSERTWQGSESSGSVGENNYIDKGSSTIGQDTSNAEERGIIRNEASSIG 240

Query: 241 LLEEALEEEKAARASLYMELEEERAAAATAADEAIAMIIRLQNEKASVEMEARQYQRVIE 300
           LLEEALEEEKAARASLYMELEEERAAAATAADEAIAMIIRLQNEKASVEMEARQYQRVIE
Sbjct: 241 LLEEALEEEKAARASLYMELEEERAAAATAADEAIAMIIRLQNEKASVEMEARQYQRVIE 300

Query: 301 EKFAYDEEEMNLLREILVKREIDYHVLEKEIEAYREMDFSEEEQSIRNWDFILDDHKEQS 360
           EKFAYDEEEMNLLREILVKREIDYHVLEKEIEAYREMDFSEEEQSIRNWDFILDDHKEQS
Sbjct: 301 EKFAYDEEEMNLLREILVKREIDYHVLEKEIEAYREMDFSEEEQSIRNWDFILDDHKEQS 360

Query: 361 ATTHYSNGDPPIVQQIENAISLSRKAKLNETKSYNSQCHFNEGGLLEQTTWTNEDNELKD 420
           ATTHYSNGDPPIVQQIENAISLSRKAKLNETKSYNSQCHFNEGGLLEQTTWTNEDNELKD
Sbjct: 361 ATTHYSNGDPPIVQQIENAISLSRKAKLNETKSYNSQCHFNEGGLLEQTTWTNEDNELKD 420

Query: 421 NSLVCEHFTSKAAHVVRSLGSSELDMEIDVQDIHVIDEKLHLGDKKEDIKVDN 474
           NSLVCEHF +KAAHVVRSLGSSELDMEIDVQDIHVIDEKLHLGDKKEDIKVDN
Sbjct: 421 NSLVCEHFATKAAHVVRSLGSSELDMEIDVQDIHVIDEKLHLGDKKEDIKVDN 473

BLAST of MS002456 vs. NCBI nr
Match: XP_038877576.1 (uncharacterized protein LOC120069830 isoform X1 [Benincasa hispida])

HSP 1 Score: 646.4 bits (1666), Expect = 2.0e-181
Identity = 349/515 (67.77%), Postives = 399/515 (77.48%), Query Frame = 0

Query: 1   MSLHEIHPWTLSGLLGAFLDLALVYVLLCISATAFIPCKILKMIGLFLPCPCSGFYGRQN 60
           MS HEIH WTLSGL+ AFLDL +VY LLC+SAT FIP KILK++G  LPCPCSGFYG  N
Sbjct: 1   MSFHEIHSWTLSGLIRAFLDLTVVYFLLCVSATVFIPSKILKLVGFCLPCPCSGFYGNYN 60

Query: 61  ANLCFRRLLFNWPKRKISSVLHFVKTRFPFDLLLVDDQIGNSNRKL-----QTKGVPQSQ 120
           ANLCF RL+ NWPKRKI  VL  VK RFPFDL+L+D+Q+ NSNR L        G+ Q Q
Sbjct: 61  ANLCFHRLIVNWPKRKIYLVLDLVKNRFPFDLILMDEQMRNSNRNLLRENHHVDGISQFQ 120

Query: 121 -------------QNLADKDGDYDRKGKRVMYQRPRTKIRRGRRAAVECGRLARGICDSN 180
                        QNL DKD +YD KGKR+MYQRP+TKIRR RRAA++ G+L++GIC+ N
Sbjct: 121 SEVCCSTFSAPRLQNLVDKDSEYDGKGKRIMYQRPKTKIRRRRRAAMDNGKLSKGICEGN 180

Query: 181 EARKERESVALVERDEFIHDVINESNHIHLSERTWQGSESSGSVGENNYIDKGSSTIGQD 240
           E  KERE VALVER +FI D +NESNH+ L +RTWQG ESSGS GENN+++K SST+GQ 
Sbjct: 181 ETGKEREFVALVERQDFITDDVNESNHMDLGQRTWQGFESSGSFGENNHMNKSSSTMGQG 240

Query: 241 TSNAEERGIIRNEASSIGLLEEALEEEKAARASLYMELEEERAAAATAADEAIAMIIRLQ 300
           TSNAEER IIRNEASSI LLE+ALEEE+AARASL++ELEEERAAAATAADEAIAMI RLQ
Sbjct: 241 TSNAEERDIIRNEASSIRLLEQALEEERAARASLFVELEEERAAAATAADEAIAMITRLQ 300

Query: 301 NEKASVEMEARQYQRVIEEKFAYDEEEMNLLREILVKREIDYHVLEKEIEAYREMDFSEE 360
           NEKASVEMEARQY RVIEEKFAYDEE++N+LREILVKR+IDYHVLEKEIEAYR+MDFSE+
Sbjct: 301 NEKASVEMEARQYHRVIEEKFAYDEEQVNILREILVKRDIDYHVLEKEIEAYRQMDFSEK 360

Query: 361 EQSIRNWDFILDDHKEQSATTHYSNGDPPIVQQIENAISLSRKAKLNETKSYNSQCHFNE 420
           EQ   NWDF+LD+H EQS T HYSNGDPPIV QI NAIS SRKAKLNET S  SQCHFNE
Sbjct: 361 EQLKMNWDFVLDEHNEQSPTAHYSNGDPPIVHQIGNAISFSRKAKLNETNSCTSQCHFNE 420

Query: 421 GGLLEQTTWTNEDNELKDNSLVCEHFTSKAA------------------------HVVRS 474
            G L+QT W +++NELKDNSL+C+H   +AA                        H V  
Sbjct: 421 EGFLKQTIWMDKNNELKDNSLLCDHIAIEAAPRCGGFDKSFLSRGALQESLEHMDHAVND 480

BLAST of MS002456 vs. NCBI nr
Match: XP_038877577.1 (uncharacterized protein LOC120069830 isoform X2 [Benincasa hispida])

HSP 1 Score: 624.4 bits (1609), Expect = 8.0e-175
Identity = 342/515 (66.41%), Postives = 390/515 (75.73%), Query Frame = 0

Query: 1   MSLHEIHPWTLSGLLGAFLDLALVYVLLCISATAFIPCKILKMIGLFLPCPCSGFYGRQN 60
           MS HEIH WTLSGL+ AFLDL +VY LLC+SAT FIP KILK++G  LPCPCSGFYG  N
Sbjct: 1   MSFHEIHSWTLSGLIRAFLDLTVVYFLLCVSATVFIPSKILKLVGFCLPCPCSGFYGNYN 60

Query: 61  ANLCFRRLLFNWPKRKISSVLHFVKTRFPFDLLLVDDQIGNSNRKL-----QTKGVPQSQ 120
           ANLCF RL+ NWPKRKI  VL  VK RFPFDL+L+D+Q+ NSNR L        G+ Q Q
Sbjct: 61  ANLCFHRLIVNWPKRKIYLVLDLVKNRFPFDLILMDEQMRNSNRNLLRENHHVDGISQFQ 120

Query: 121 -------------QNLADKDGDYDRKGKRVMYQRPRTKIRRGRRAAVECGRLARGICDSN 180
                        QNL DKD +YD KGKR+MYQRP+TKIRR RRAA++ G+L++GIC+ N
Sbjct: 121 SEVCCSTFSAPRLQNLVDKDSEYDGKGKRIMYQRPKTKIRRRRRAAMDNGKLSKGICEGN 180

Query: 181 EARKERESVALVERDEFIHDVINESNHIHLSERTWQGSESSGSVGENNYIDKGSSTIGQD 240
           E  KERE VALVER +FI             +RTWQG ESSGS GENN+++K SST+GQ 
Sbjct: 181 ETGKEREFVALVERQDFI-----------TGQRTWQGFESSGSFGENNHMNKSSSTMGQG 240

Query: 241 TSNAEERGIIRNEASSIGLLEEALEEEKAARASLYMELEEERAAAATAADEAIAMIIRLQ 300
           TSNAEER IIRNEASSI LLE+ALEEE+AARASL++ELEEERAAAATAADEAIAMI RLQ
Sbjct: 241 TSNAEERDIIRNEASSIRLLEQALEEERAARASLFVELEEERAAAATAADEAIAMITRLQ 300

Query: 301 NEKASVEMEARQYQRVIEEKFAYDEEEMNLLREILVKREIDYHVLEKEIEAYREMDFSEE 360
           NEKASVEMEARQY RVIEEKFAYDEE++N+LREILVKR+IDYHVLEKEIEAYR+MDFSE+
Sbjct: 301 NEKASVEMEARQYHRVIEEKFAYDEEQVNILREILVKRDIDYHVLEKEIEAYRQMDFSEK 360

Query: 361 EQSIRNWDFILDDHKEQSATTHYSNGDPPIVQQIENAISLSRKAKLNETKSYNSQCHFNE 420
           EQ   NWDF+LD+H EQS T HYSNGDPPIV QI NAIS SRKAKLNET S  SQCHFNE
Sbjct: 361 EQLKMNWDFVLDEHNEQSPTAHYSNGDPPIVHQIGNAISFSRKAKLNETNSCTSQCHFNE 420

Query: 421 GGLLEQTTWTNEDNELKDNSLVCEHFTSKAA------------------------HVVRS 474
            G L+QT W +++NELKDNSL+C+H   +AA                        H V  
Sbjct: 421 EGFLKQTIWMDKNNELKDNSLLCDHIAIEAAPRCGGFDKSFLSRGALQESLEHMDHAVND 480

BLAST of MS002456 vs. NCBI nr
Match: XP_022953644.1 (probable myosin-binding protein 6 [Cucurbita moschata])

HSP 1 Score: 560.1 bits (1442), Expect = 1.9e-155
Identity = 317/496 (63.91%), Postives = 366/496 (73.79%), Query Frame = 0

Query: 1   MSLHEIHPWTLSGLLGAFLDLALVYVLLCISATAFIPCKILKMIGLFLPCPCSGFYGRQN 60
           M+ HEIH WT  GL+ AFLDLA+VY LLC+SAT FIP KIL+++G  LPCPC+GFYG QN
Sbjct: 1   MTFHEIHSWTFCGLVRAFLDLAVVYFLLCVSATVFIPSKILEVVGFCLPCPCTGFYGNQN 60

Query: 61  ANLCFRRLLFNWPKRKISSVLHFVKTRFPFDLLLVDDQIGNSNRKLQ----TKGVPQSQQ 120
            NLC  RLLF+WPKRKI  VL  VK RFPFDL+LVDDQ+G +   L+    T GVP  Q 
Sbjct: 61  PNLCLHRLLFSWPKRKIYLVLDSVKHRFPFDLILVDDQMGKNRNLLRENHHTDGVPLLQS 120

Query: 121 NLADKDGDYDRKGKRVMYQRPRTKIRRGRRAAVECGRLARGICDSNEARKERESVALVER 180
                      KG R+M+QRPR      RRA+VE G+L          RKE E +AL ++
Sbjct: 121 AACCS------KGNRIMFQRPRR-----RRASVEYGKL---------FRKEWEFLALDQK 180

Query: 181 DEFIHDVINESNHIHLSERTWQGSESSGSVGENNYIDKGSSTIGQDTSNAEERGIIRNEA 240
            +FI D +NES H+ L  RTWQG ESSGSVGEN+Y++KGSSTIG  T+N +ER I  NE 
Sbjct: 181 QDFIPDDVNESYHMDLGRRTWQGFESSGSVGENSYLNKGSSTIGHGTNNDQERDITGNEV 240

Query: 241 SSIGLLEEALEEEKAARASLYMELEEERAAAATAADEAIAMIIRLQNEKASVEMEARQYQ 300
            SI LLE+ALEEEKAARASL++ELEEERAAAATAADEAIAMI RLQNEKASVEMEARQYQ
Sbjct: 241 -SIRLLEQALEEEKAARASLFLELEEERAAAATAADEAIAMITRLQNEKASVEMEARQYQ 300

Query: 301 RVIEEKFAYDEEEMNLLREILVKREIDYHVLEKEIEAYREMDFSEEEQSIRNWDFILDDH 360
           RVIEEKFAYDEEEMN+LREILV+REIDYHVLEKEIEAYR+MDFSE+E+  RNWDFILD+H
Sbjct: 301 RVIEEKFAYDEEEMNILREILVQREIDYHVLEKEIEAYRQMDFSEKEELKRNWDFILDEH 360

Query: 361 KEQSATTHYSNGDPPIVQQIENAISLSRKAKLNETKSYNSQCHFNEGGLLEQTTWTNEDN 420
           KEQS+T  YSNGDPP+V QIENA+SLS KAK NE+ S NSQCHFNE GLL+QT WT++DN
Sbjct: 361 KEQSSTARYSNGDPPVVHQIENALSLSSKAKSNESNSCNSQCHFNEEGLLKQTIWTDKDN 420

Query: 421 ELKDNSLVCEHFTSKAA------------------------HVVRSLGSSELDMEIDVQD 469
           EL DNSL+ E    + A                        H +  LGSS LDMEIDVQD
Sbjct: 421 ELMDNSLLFERTAIEGALRRGGFEKSVLSRGALQNRLEHIDHTINDLGSSILDMEIDVQD 475

BLAST of MS002456 vs. NCBI nr
Match: KAG6575980.1 (Myosin-binding protein 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 558.5 bits (1438), Expect = 5.4e-155
Identity = 316/496 (63.71%), Postives = 366/496 (73.79%), Query Frame = 0

Query: 1   MSLHEIHPWTLSGLLGAFLDLALVYVLLCISATAFIPCKILKMIGLFLPCPCSGFYGRQN 60
           M+ HEIH WT  GL+ AFLDLA+VY LLC+SAT FIP KIL+++G  LPCPC+GFYG QN
Sbjct: 1   MTFHEIHSWTFCGLVRAFLDLAVVYFLLCVSATVFIPSKILEVVGFCLPCPCTGFYGNQN 60

Query: 61  ANLCFRRLLFNWPKRKISSVLHFVKTRFPFDLLLVDDQIGNSNRKLQ----TKGVPQSQQ 120
            NLC  RLLF+WPKRKI  VL  VK RFPFDL+LVDDQ+G +   L+    T GVP  Q 
Sbjct: 61  PNLCLHRLLFSWPKRKIYLVLDSVKHRFPFDLILVDDQMGKNRNLLRENHHTDGVPLLQS 120

Query: 121 NLADKDGDYDRKGKRVMYQRPRTKIRRGRRAAVECGRLARGICDSNEARKERESVALVER 180
                      KG R+M+QRPR      RRA+VE G+L          RKE E +AL ++
Sbjct: 121 AACCS------KGNRIMFQRPRR-----RRASVEYGKL---------FRKEWEFLALDQK 180

Query: 181 DEFIHDVINESNHIHLSERTWQGSESSGSVGENNYIDKGSSTIGQDTSNAEERGIIRNEA 240
            +FI D +NES H+ L  RTWQG ESSGSVGEN+Y++KGSSTIG  T+N +ER I  NE 
Sbjct: 181 QDFIPDDVNESYHMDLGRRTWQGFESSGSVGENSYLNKGSSTIGHGTNNDQERDITGNEV 240

Query: 241 SSIGLLEEALEEEKAARASLYMELEEERAAAATAADEAIAMIIRLQNEKASVEMEARQYQ 300
            SI LLE+ALEEEKAARASL++ELEEERAAAATAADEAIAMI RLQNEKASVEMEARQYQ
Sbjct: 241 -SIRLLEQALEEEKAARASLFLELEEERAAAATAADEAIAMITRLQNEKASVEMEARQYQ 300

Query: 301 RVIEEKFAYDEEEMNLLREILVKREIDYHVLEKEIEAYREMDFSEEEQSIRNWDFILDDH 360
           RVIEEKFAYDEEEMN+LREILV+REIDYHVLEKEIEAYR+MDFSE+E+  RNWDFILD+H
Sbjct: 301 RVIEEKFAYDEEEMNILREILVQREIDYHVLEKEIEAYRQMDFSEKEELKRNWDFILDEH 360

Query: 361 KEQSATTHYSNGDPPIVQQIENAISLSRKAKLNETKSYNSQCHFNEGGLLEQTTWTNEDN 420
           KEQS+T  YSNGDPP+V QIENA+SLS KAK NE+ S NSQCHFNE GLL+QT WT++DN
Sbjct: 361 KEQSSTARYSNGDPPVVHQIENALSLSSKAKSNESNSCNSQCHFNEEGLLKQTIWTDKDN 420

Query: 421 ELKDNSLVCEHFTSKAA------------------------HVVRSLGSSELDMEIDVQD 469
           E+ DNSL+ E    + A                        H +  LGSS LDMEIDVQD
Sbjct: 421 EVMDNSLLFERSAIEEALRRGGFEKSVLSRGALQNRLEHIDHTINDLGSSILDMEIDVQD 475

BLAST of MS002456 vs. ExPASy Swiss-Prot
Match: Q9CAC4 (Myosin-binding protein 2 OS=Arabidopsis thaliana OX=3702 GN=MYOB2 PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 1.5e-14
Identity = 55/132 (41.67%), Postives = 85/132 (64.39%), Query Frame = 0

Query: 238 SIGLLEEALEEEKAARASLYMELEEERAAAATAADEAIAMIIRLQNEKASVEMEARQYQR 297
           ++  L+  L+EE+ A  +LY ELE ER A+A AA E +AMI RL  EKA+++MEA QYQR
Sbjct: 412 TVDKLKFELQEERKALHALYEELEVERNASAVAASETMAMINRLHEEKAAMQMEALQYQR 471

Query: 298 VIEEKFAYDEEEMNLLREILVKREIDYHVLEKEIEAYREMDFSEEEQSIRNWDFILDDHK 357
           ++EE+  +D+E + LL E++V RE +   LEKE+E YR+    EE ++      +    +
Sbjct: 472 MMEEQAEFDQEALQLLNELMVNREKENAELEKELEVYRKR--MEEYEAKEKMGMLRRRLR 531

Query: 358 EQSATTHYSNGD 370
           + S  ++ +NGD
Sbjct: 532 DSSVDSYRNNGD 541

BLAST of MS002456 vs. ExPASy Swiss-Prot
Match: Q0WNW4 (Myosin-binding protein 3 OS=Arabidopsis thaliana OX=3702 GN=MYOB3 PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.9e-14
Identity = 56/129 (43.41%), Postives = 81/129 (62.79%), Query Frame = 0

Query: 238 SIGLLEEALEEEKAARASLYMELEEERAAAATAADEAIAMIIRLQNEKASVEMEARQYQR 297
           +I  L E +  E+ A   LY ELEEER+A+A +A++ +AMI RLQ EKA V+MEA QYQR
Sbjct: 356 TIERLRETVRAEQEALRDLYAELEEERSASAISANQTMAMITRLQEEKAKVQMEALQYQR 415

Query: 298 VIEEKFAYDEEEMNLLREILVKREIDYHVLEKEIEAYREMDFSEEEQSIRNWDFIL---- 357
           ++EE+  YD+E + LL  ++VKRE +   L++E+E YR     E E   +N   ++    
Sbjct: 416 MMEEQAEYDQEALQLLNHLMVKREKEKEQLQRELEVYR-AKVLEYESKAKNKIIVVENDC 475

Query: 358 ---DDHKEQ 360
              DD KE+
Sbjct: 476 EADDDDKEE 483

BLAST of MS002456 vs. ExPASy Swiss-Prot
Match: F4HVS6 (Probable myosin-binding protein 6 OS=Arabidopsis thaliana OX=3702 GN=MYOB6 PE=2 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 1.6e-13
Identity = 64/180 (35.56%), Postives = 98/180 (54.44%), Query Frame = 0

Query: 237 SSIGLLEEALEEEKAARASLYMELEEERAAAATAADEAIAMIIRLQNEKASVEMEARQYQ 296
           S +  L++ +  +K +   LYMEL+EER+A+A AA+EA+AMI RLQ EKA+V+MEA QYQ
Sbjct: 300 SILNQLKKEVRLDKKSLIDLYMELDEERSASAVAANEAMAMITRLQAEKAAVQMEALQYQ 359

Query: 297 RVIEEKFAYDEEEMNLLREILVKREIDYHVLEKEIEAYREMDFSEEEQSIRNWDFILDDH 356
           R+++E+  YD+E +  +   L KRE +   LE E E YRE      +Q     +F    H
Sbjct: 360 RMMDEQAEYDQEALQSMSSELAKREEEMKELEAEFEVYREKYGCLTDQEDAREEF----H 419

Query: 357 KEQSATTHYSNGDPPIVQQIEN-AISLSRKAKLNETKSYNSQCHFNEGGLLEQTTWTNED 416
           K+    + Y   D    + + + A+S S + +  E    N Q   +E    E     +E+
Sbjct: 420 KQNGNASAYD--DCQETKPVSDLAVSSSNQQENGENIDQNGQSKRSEESTAENVVSADEE 473

BLAST of MS002456 vs. ExPASy Swiss-Prot
Match: Q9LMC8 (Probable myosin-binding protein 5 OS=Arabidopsis thaliana OX=3702 GN=MYOB5 PE=2 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 1.8e-12
Identity = 46/94 (48.94%), Postives = 66/94 (70.21%), Query Frame = 0

Query: 242 LEEALEEEKAARASLYMELEEERAAAATAADEAIAMIIRLQNEKASVEMEARQYQRVIEE 301
           L   +  ++ +   LYMEL+EER+A+A AA+ A+AMI RLQ EKA+V+MEA QYQR+++E
Sbjct: 304 LNRQVRLDRKSLMDLYMELDEERSASAVAANNAMAMITRLQAEKAAVQMEALQYQRMMDE 363

Query: 302 KFAYDEEEMNLLREILVKREIDYHVLEKEIEAYR 336
           +  YD+E +  +  +LVKRE +   LE  IE YR
Sbjct: 364 QAEYDQEALQSMNGLLVKREEEMKELEAGIEVYR 397

BLAST of MS002456 vs. ExPASy Swiss-Prot
Match: Q9FG14 (Myosin-binding protein 7 OS=Arabidopsis thaliana OX=3702 GN=MYOB7 PE=1 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 6.9e-12
Identity = 65/183 (35.52%), Postives = 102/183 (55.74%), Query Frame = 0

Query: 223 TSNAEERGIIRNEASSIGLLEEALEEEKAARASLYMELEEERAAAATAADEAIAMIIRLQ 282
           +SNA+ +  I NE   + LL E +  ++ +   LY EL+EER AA+TAA EA++MI+RLQ
Sbjct: 60  SSNAKVQ--IENE---LELLRETVSSQQQSIQDLYEELDEERNAASTAASEAMSMILRLQ 119

Query: 283 NEKASVEMEARQYQRVIEEKFAYDEEEMNLLREILVKREIDYHVLEKEIEAY--REMDF- 342
            +KA ++ME RQ++R  EEK  +D++E+  L +++ KRE     L  E +AY  R M F 
Sbjct: 120 RDKAELQMELRQFKRFAEEKMEHDQQELLDLEDLIYKREQTIQALTFEAQAYKHRMMSFG 179

Query: 343 -------SEEEQSIRNWDFILDDHKEQSATTHYSNGDPPIVQQI-ENAISLSRKAKLNET 395
                  +E+    RN   I +D++    T+ Y    PPI   + EN   L     +++ 
Sbjct: 180 FTEAEVETEKNMLSRNPSMIENDYQYDLPTSDY----PPIKCNVNENPGPLEADIDVDDV 233

BLAST of MS002456 vs. ExPASy TrEMBL
Match: A0A6J1D8Y8 (uncharacterized protein LOC111018343 OS=Momordica charantia OX=3673 GN=LOC111018343 PE=4 SV=1)

HSP 1 Score: 906.4 bits (2341), Expect = 5.1e-260
Identity = 470/473 (99.37%), Postives = 472/473 (99.79%), Query Frame = 0

Query: 1   MSLHEIHPWTLSGLLGAFLDLALVYVLLCISATAFIPCKILKMIGLFLPCPCSGFYGRQN 60
           MSLHEIHPWTLSGLLGAFLDLALVYVLLCISATAFIPCKILKMIGLFLPCPCSGFYGRQN
Sbjct: 1   MSLHEIHPWTLSGLLGAFLDLALVYVLLCISATAFIPCKILKMIGLFLPCPCSGFYGRQN 60

Query: 61  ANLCFRRLLFNWPKRKISSVLHFVKTRFPFDLLLVDDQIGNSNRKLQTKGVPQSQQNLAD 120
           ANLCFRRLLFNWPKRKISSVLHFVKTRFPFDLLLVDDQIGNSNRKLQTKGVPQSQQNLAD
Sbjct: 61  ANLCFRRLLFNWPKRKISSVLHFVKTRFPFDLLLVDDQIGNSNRKLQTKGVPQSQQNLAD 120

Query: 121 KDGDYDRKGKRVMYQRPRTKIRRGRRAAVECGRLARGICDSNEARKERESVALVERDEFI 180
           +DGDYDRKGKRVMYQRPRTKIRRGRRAAVECGRLARGICDSNEARKERESVALVERDEFI
Sbjct: 121 RDGDYDRKGKRVMYQRPRTKIRRGRRAAVECGRLARGICDSNEARKERESVALVERDEFI 180

Query: 181 HDVINESNHIHLSERTWQGSESSGSVGENNYIDKGSSTIGQDTSNAEERGIIRNEASSIG 240
           HDVINESNHIHLSERTWQGSESSGSVGENNYIDKGSSTIGQDTSNAEERGIIRNEASSIG
Sbjct: 181 HDVINESNHIHLSERTWQGSESSGSVGENNYIDKGSSTIGQDTSNAEERGIIRNEASSIG 240

Query: 241 LLEEALEEEKAARASLYMELEEERAAAATAADEAIAMIIRLQNEKASVEMEARQYQRVIE 300
           LLEEALEEEKAARASLYMELEEERAAAATAADEAIAMIIRLQNEKASVEMEARQYQRVIE
Sbjct: 241 LLEEALEEEKAARASLYMELEEERAAAATAADEAIAMIIRLQNEKASVEMEARQYQRVIE 300

Query: 301 EKFAYDEEEMNLLREILVKREIDYHVLEKEIEAYREMDFSEEEQSIRNWDFILDDHKEQS 360
           EKFAYDEEEMNLLREILVKREIDYHVLEKEIEAYREMDFSEEEQSIRNWDFILDDHKEQS
Sbjct: 301 EKFAYDEEEMNLLREILVKREIDYHVLEKEIEAYREMDFSEEEQSIRNWDFILDDHKEQS 360

Query: 361 ATTHYSNGDPPIVQQIENAISLSRKAKLNETKSYNSQCHFNEGGLLEQTTWTNEDNELKD 420
           ATTHYSNGDPPIVQQIENAISLSRKAKLNETKSYNSQCHFNEGGLLEQTTWTNEDNELKD
Sbjct: 361 ATTHYSNGDPPIVQQIENAISLSRKAKLNETKSYNSQCHFNEGGLLEQTTWTNEDNELKD 420

Query: 421 NSLVCEHFTSKAAHVVRSLGSSELDMEIDVQDIHVIDEKLHLGDKKEDIKVDN 474
           NSLVCEHF +KAAHVVRSLGSSELDMEIDVQDIHVIDEKLHLGDKKEDIKVDN
Sbjct: 421 NSLVCEHFATKAAHVVRSLGSSELDMEIDVQDIHVIDEKLHLGDKKEDIKVDN 473

BLAST of MS002456 vs. ExPASy TrEMBL
Match: A0A6J1GQ95 (probable myosin-binding protein 6 OS=Cucurbita moschata OX=3662 GN=LOC111456111 PE=4 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 9.0e-156
Identity = 317/496 (63.91%), Postives = 366/496 (73.79%), Query Frame = 0

Query: 1   MSLHEIHPWTLSGLLGAFLDLALVYVLLCISATAFIPCKILKMIGLFLPCPCSGFYGRQN 60
           M+ HEIH WT  GL+ AFLDLA+VY LLC+SAT FIP KIL+++G  LPCPC+GFYG QN
Sbjct: 1   MTFHEIHSWTFCGLVRAFLDLAVVYFLLCVSATVFIPSKILEVVGFCLPCPCTGFYGNQN 60

Query: 61  ANLCFRRLLFNWPKRKISSVLHFVKTRFPFDLLLVDDQIGNSNRKLQ----TKGVPQSQQ 120
            NLC  RLLF+WPKRKI  VL  VK RFPFDL+LVDDQ+G +   L+    T GVP  Q 
Sbjct: 61  PNLCLHRLLFSWPKRKIYLVLDSVKHRFPFDLILVDDQMGKNRNLLRENHHTDGVPLLQS 120

Query: 121 NLADKDGDYDRKGKRVMYQRPRTKIRRGRRAAVECGRLARGICDSNEARKERESVALVER 180
                      KG R+M+QRPR      RRA+VE G+L          RKE E +AL ++
Sbjct: 121 AACCS------KGNRIMFQRPRR-----RRASVEYGKL---------FRKEWEFLALDQK 180

Query: 181 DEFIHDVINESNHIHLSERTWQGSESSGSVGENNYIDKGSSTIGQDTSNAEERGIIRNEA 240
            +FI D +NES H+ L  RTWQG ESSGSVGEN+Y++KGSSTIG  T+N +ER I  NE 
Sbjct: 181 QDFIPDDVNESYHMDLGRRTWQGFESSGSVGENSYLNKGSSTIGHGTNNDQERDITGNEV 240

Query: 241 SSIGLLEEALEEEKAARASLYMELEEERAAAATAADEAIAMIIRLQNEKASVEMEARQYQ 300
            SI LLE+ALEEEKAARASL++ELEEERAAAATAADEAIAMI RLQNEKASVEMEARQYQ
Sbjct: 241 -SIRLLEQALEEEKAARASLFLELEEERAAAATAADEAIAMITRLQNEKASVEMEARQYQ 300

Query: 301 RVIEEKFAYDEEEMNLLREILVKREIDYHVLEKEIEAYREMDFSEEEQSIRNWDFILDDH 360
           RVIEEKFAYDEEEMN+LREILV+REIDYHVLEKEIEAYR+MDFSE+E+  RNWDFILD+H
Sbjct: 301 RVIEEKFAYDEEEMNILREILVQREIDYHVLEKEIEAYRQMDFSEKEELKRNWDFILDEH 360

Query: 361 KEQSATTHYSNGDPPIVQQIENAISLSRKAKLNETKSYNSQCHFNEGGLLEQTTWTNEDN 420
           KEQS+T  YSNGDPP+V QIENA+SLS KAK NE+ S NSQCHFNE GLL+QT WT++DN
Sbjct: 361 KEQSSTARYSNGDPPVVHQIENALSLSSKAKSNESNSCNSQCHFNEEGLLKQTIWTDKDN 420

Query: 421 ELKDNSLVCEHFTSKAA------------------------HVVRSLGSSELDMEIDVQD 469
           EL DNSL+ E    + A                        H +  LGSS LDMEIDVQD
Sbjct: 421 ELMDNSLLFERTAIEGALRRGGFEKSVLSRGALQNRLEHIDHTINDLGSSILDMEIDVQD 475

BLAST of MS002456 vs. ExPASy TrEMBL
Match: A0A0A0K8C0 (GTD-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G339690 PE=4 SV=1)

HSP 1 Score: 555.1 bits (1429), Expect = 2.9e-154
Identity = 314/510 (61.57%), Postives = 368/510 (72.16%), Query Frame = 0

Query: 1   MSLHEIHPWTLSGLLGAFLDLALVYVLLCISATAFIPCKILKMIGLFLPCPCSGFYGRQN 60
           MS HEIH WTLSG++ AFLDLA+VY LLC+SAT FIP KILK++G  LPCPC+GFYG  N
Sbjct: 1   MSFHEIHSWTLSGIVRAFLDLAVVYFLLCVSATMFIPSKILKVVGFCLPCPCTGFYGNHN 60

Query: 61  ANLCFRRLLFNWPKRKISSVLHFVKTRFPFDLLLVDDQ-IGNSNRK-LQTKGVPQSQ--- 120
            NLCF +L+ NWPKRKI  VL  VK  FPFDL+L+DD+ +GNSNR  L+  G+ + Q   
Sbjct: 61  TNLCFHKLVVNWPKRKIYLVLDLVKNMFPFDLILMDDEKVGNSNRNLLRENGISRLQSEV 120

Query: 121 --------QNLADKDGDYDRKGKRVMYQRPRTKIRRGRRAAVECGRLARGICDSNEARKE 180
                   QNL D DG++D KGK++MYQ+PRTKIRR RRA ++ G+L++G+C+ NE RK 
Sbjct: 121 CCSTAPRLQNLVDNDGEHDGKGKKIMYQKPRTKIRRRRRATIDNGKLSKGVCEENETRKG 180

Query: 181 RESVALVERDEFIHDVINESNHIHLSERTWQGSESSGSVGENNYIDKGSSTIGQDTSNAE 240
           RE VALVER +FI D  NESNH+ L +R WQG ESSGS+GEN+Y++KGSST+GQ TS AE
Sbjct: 181 REFVALVERQDFITDDGNESNHLDLGQRIWQGFESSGSLGENSYMNKGSSTVGQGTSKAE 240

Query: 241 ERGIIRNEASSIGLLEEALEEEKAARASLYMELEEERAAAATAADEAIAMIIRLQNEKAS 300
           ER IIRNEAS+I LLE ALEEE+ ARASL++ELEEERAAAATAADEAIAMI RLQNEKAS
Sbjct: 241 ERAIIRNEASTIRLLELALEEERTARASLFVELEEERAAAATAADEAIAMITRLQNEKAS 300

Query: 301 VEMEARQYQRVIEEKFAYDEEEMNLLREILVKREIDYHVLEKEIEAYREMDFSEEEQSIR 360
            EMEARQY R +EEKF+YDEE+MN+LREILVKR+IDYHVLEKEIEAYR+MDF+EEE    
Sbjct: 301 FEMEARQYHREVEEKFSYDEEKMNILREILVKRDIDYHVLEKEIEAYRQMDFAEEELLKG 360

Query: 361 NWDFILDDHKEQSATTHYSNGDPPIVQQIENAISLSRKAKLNETKSYNSQCHFNEGGLLE 420
           N DFILD+H E SAT HYSNGDPPIV  I NA+SLSR+AKL                   
Sbjct: 361 NQDFILDEHTELSATAHYSNGDPPIVYPIGNAVSLSRRAKL------------------- 420

Query: 421 QTTWTNEDNELKDNSLVCEHFTSKAA-----------------------HVVRSLGSSEL 474
                   NEL+DNSL+ +H    AA                       H V  LG S L
Sbjct: 421 --------NELRDNSLLSDHIAIDAAPHCGGFEKSFLSRGALQNLEHITHAVNDLGDSIL 480

BLAST of MS002456 vs. ExPASy TrEMBL
Match: A0A1S3C7F2 (uncharacterized protein LOC103497547 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103497547 PE=4 SV=1)

HSP 1 Score: 552.7 bits (1423), Expect = 1.4e-153
Identity = 315/495 (63.64%), Postives = 365/495 (73.74%), Query Frame = 0

Query: 1   MSLHEIHPWTLSGLLGAFLDLALVYVLLCISATAFIPCKILKMIGLFLPCPCSGFYGRQN 60
           MS HEIH WTLSGL+ AFLDLA+VY LLC+SAT FIP KILK++G  LPCPC+GFYG  N
Sbjct: 1   MSFHEIHSWTLSGLVRAFLDLAVVYFLLCVSATMFIPSKILKVVGFCLPCPCTGFYGNHN 60

Query: 61  ANLCFRRLLFNWPKRKISSVLHFVKTRFPFDLLLVD-DQIGNSNRK-LQTKGVPQSQ--- 120
            NLC  +L+ NWPKRKI  VL  VK RFPFDL  +D +QIGNSNR  L+  G+ + Q   
Sbjct: 61  TNLCLHKLVVNWPKRKIYLVLDLVKNRFPFDLFFMDAEQIGNSNRNMLRENGISRLQSEV 120

Query: 121 --------QNLADKDGDYDRKGKRVMYQRPRTKIRRGRRAAVECGRLARGICDSNEARKE 180
                   QN+ DK G+YD KGK++MYQ+PRTKIRR RRAAV+ G+L++GIC+ NE RKE
Sbjct: 121 CCSTVPRLQNMVDKYGEYDGKGKKMMYQKPRTKIRRRRRAAVDNGKLSKGICEGNETRKE 180

Query: 181 RESVALVERDEFIHDVINESNHIHLSERTWQGSESSGSVGENNYIDKGSSTIGQDTSNAE 240
           RE VALVER +FI D  NESNH  L +R WQG ESSGS+GENNY++KGSST+GQ TSNAE
Sbjct: 181 REFVALVERQDFITDDGNESNHPDLGQRIWQGFESSGSLGENNYMNKGSSTVGQGTSNAE 240

Query: 241 ERGIIRNEASSIGLLEEALEEEKAARASLYMELEEERAAAATAADEAIAMIIRLQNEKAS 300
           ER IIRNEAS+I LLE ALEEE+AARASL++ELEEERAAAATAADEAIAMI RLQNEKAS
Sbjct: 241 ERDIIRNEASTIRLLELALEEERAARASLFVELEEERAAAATAADEAIAMITRLQNEKAS 300

Query: 301 VEMEARQYQRVIEEKFAYDEEEMNLLREILVKREIDYHVLEKEIEAYREMDFSEEEQSIR 360
            EMEARQY R +EEKF+YDEE+MN+LREILVKR+IDYHVLEKEIEAYR+MDF+E+EQ   
Sbjct: 301 FEMEARQYHREVEEKFSYDEEKMNILREILVKRDIDYHVLEKEIEAYRQMDFAEKEQLKG 360

Query: 361 NWDFILDDHKEQSATTHYSNGDPPIVQQIENAISLSRKAKLNETKSYN---------SQC 420
           N D+ILD+HKE S T H SNGDPPI   I NA+SL R  KLNE +  +           C
Sbjct: 361 NRDYILDEHKELSVTAHCSNGDPPIGYPIGNAVSLQRTVKLNELRDNSLLSDAIEAAPHC 420

Query: 421 HFNEGGLLEQTTWTNEDNELKDNSLVCEHFTSKAAHVVRSLGSSELDMEIDVQDIHVIDE 474
              E   L +    N D           H T    H V  LG S +DMEIDVQDIHVIDE
Sbjct: 421 GGFEKSFLSRGALQNLD-----------HIT----HAVNDLGGSIVDMEIDVQDIHVIDE 480

BLAST of MS002456 vs. ExPASy TrEMBL
Match: A0A5D3E523 (Putative myosin-binding protein 5 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold343G00070 PE=4 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 6.0e-152
Identity = 315/495 (63.64%), Postives = 364/495 (73.54%), Query Frame = 0

Query: 1   MSLHEIHPWTLSGLLGAFLDLALVYVLLCISATAFIPCKILKMIGLFLPCPCSGFYGRQN 60
           MS HEIH WTLSGL+ AFLDLA+VY LLC+SAT FIP KILK++G  LPCPC+GFYG  N
Sbjct: 1   MSFHEIHSWTLSGLVRAFLDLAVVYFLLCVSATMFIPSKILKVVGFCLPCPCTGFYGNHN 60

Query: 61  ANLCFRRLLFNWPKRKISSVLHFVKTRFPFDLLLVDD-QIGNSNRK-LQTKGVPQSQ--- 120
            NLC  +L+ NWPKRKI  VL  VK RFPFDL  +DD QIGNSNR  L+  G+ + Q   
Sbjct: 61  TNLCLHKLVVNWPKRKIYLVLDLVKNRFPFDLFFMDDEQIGNSNRNLLRENGISRLQSEV 120

Query: 121 --------QNLADKDGDYDRKGKRVMYQRPRTKIRRGRRAAVECGRLARGICDSNEARKE 180
                   QN+ DK G+YD K K++MYQ+PRTKIRR RRAAV+ G+L++GIC+ NE RKE
Sbjct: 121 CCSTVPRLQNMVDKYGEYDGKDKKMMYQKPRTKIRRRRRAAVDNGKLSKGICEGNETRKE 180

Query: 181 RESVALVERDEFIHDVINESNHIHLSERTWQGSESSGSVGENNYIDKGSSTIGQDTSNAE 240
           RE VALVER +FI D  NESNH  L +R WQG ESSGS+GENNY++KGSST+GQ  SNAE
Sbjct: 181 REFVALVERQDFITDDGNESNHPDLGQRIWQGFESSGSLGENNYMNKGSSTVGQ-ASNAE 240

Query: 241 ERGIIRNEASSIGLLEEALEEEKAARASLYMELEEERAAAATAADEAIAMIIRLQNEKAS 300
           ER IIRNEAS+I LLE ALEEE+AARASL++ELEEERAAAATAADEAIAMI RLQNEKAS
Sbjct: 241 ERDIIRNEASTIRLLELALEEERAARASLFVELEEERAAAATAADEAIAMITRLQNEKAS 300

Query: 301 VEMEARQYQRVIEEKFAYDEEEMNLLREILVKREIDYHVLEKEIEAYREMDFSEEEQSIR 360
            EMEARQY R +EEKF+YDEE+MN+LREILVKR+IDYHVLEKEIEAYR+MDF+E+EQ   
Sbjct: 301 FEMEARQYHREVEEKFSYDEEKMNILREILVKRDIDYHVLEKEIEAYRQMDFAEKEQLKG 360

Query: 361 NWDFILDDHKEQSATTHYSNGDPPIVQQIENAISLSRKAKLNETKSYN---------SQC 420
           N D+ILD+HKE SAT H SNGDPPI   I NA+SL R  KLNE +  +           C
Sbjct: 361 NRDYILDEHKELSATAHCSNGDPPIGYPIGNAVSLQRTVKLNELRDNSLLSDAIEAAPHC 420

Query: 421 HFNEGGLLEQTTWTNEDNELKDNSLVCEHFTSKAAHVVRSLGSSELDMEIDVQDIHVIDE 474
              E   L +    N D           H T    H V  LG S +DMEIDVQDIHVIDE
Sbjct: 421 GGFEKSFLSRGALQNLD-----------HIT----HAVNDLGGSIVDMEIDVQDIHVIDE 479

BLAST of MS002456 vs. TAIR 10
Match: AT4G13630.1 (Protein of unknown function, DUF593 )

HSP 1 Score: 168.7 bits (426), Expect = 1.1e-41
Identity = 136/366 (37.16%), Postives = 189/366 (51.64%), Query Frame = 0

Query: 1   MSLHEIHPWTLSGLLGAFLDLALVYVLLCISATAFIPCKILKMIGLFLPCPCSGFYGRQN 60
           M   E+  WT  GL+ AF+DL++ + LLC S   ++  K L + GL LPCPC G Y    
Sbjct: 1   MRCQEVKSWTFKGLVAAFIDLSVAFSLLCASFIVYVTSKFLGLFGLDLPCPCDGLYSE-- 60

Query: 61  ANLCFRRLLFNWPKRKISSVLHFVKTRFPFDLLLVDDQIGNSNRKLQTKGVPQSQ----- 120
              CF+  L N P +KISSV   VK R PFD +L +   G   RK + + V         
Sbjct: 61  ---CFQESLRNLPVKKISSVQRSVKNRTPFDSILYN---GGKKRKCERRRVQLEDEVSST 120

Query: 121 -------QNLAD----------KDGDYDRKGKRVMYQRPRTKIRRGRRAAVECGRLARGI 180
                  +N A           K G +  K KR+ + R     +   ++ +         
Sbjct: 121 TPSVGKFENKASGFDLLTAQSLKKGSFKVKSKRLSFHRSPYGFKNHYQSCLG-------- 180

Query: 181 CDSNEARKERESVALVERDEFIHDVINESNHIHLSERTWQGSESSGSVGENNYIDKGSST 240
             S E   +     LV          N+S    L + + + S S  SVG           
Sbjct: 181 LKSFEGSYDENDPLLVNS--------NDSGKA-LEDVSLRKSVSLSSVG----------- 240

Query: 241 IGQDTSNAEERGIIRNEASSIGLLEEALEEEKAARASLYMELEEERAAAATAADEAIAMI 300
            G         G+++    ++ + E+ L EE+AARASL +ELE+ER AAA+AADEA+ MI
Sbjct: 241 CGVGEGACSSPGMVQR---TVEMSEQVLGEERAARASLALELEKERNAAASAADEALGMI 300

Query: 301 IRLQNEKASVEMEARQYQRVIEEKFAYDEEEMNLLREILVKREIDYHVLEKEIEAYREMD 345
           +RLQ EKAS+EMEARQYQR+IEEK A+D EEM++L+EIL++RE + H LEKE++ YR+M 
Sbjct: 301 LRLQEEKASIEMEARQYQRMIEEKSAFDAEEMSILKEILLRREREKHFLEKEVDTYRQM- 326

BLAST of MS002456 vs. TAIR 10
Match: AT4G13630.2 (Protein of unknown function, DUF593 )

HSP 1 Score: 168.7 bits (426), Expect = 1.1e-41
Identity = 136/366 (37.16%), Postives = 189/366 (51.64%), Query Frame = 0

Query: 1   MSLHEIHPWTLSGLLGAFLDLALVYVLLCISATAFIPCKILKMIGLFLPCPCSGFYGRQN 60
           M   E+  WT  GL+ AF+DL++ + LLC S   ++  K L + GL LPCPC G Y    
Sbjct: 1   MRCQEVKSWTFKGLVAAFIDLSVAFSLLCASFIVYVTSKFLGLFGLDLPCPCDGLYSE-- 60

Query: 61  ANLCFRRLLFNWPKRKISSVLHFVKTRFPFDLLLVDDQIGNSNRKLQTKGVPQSQ----- 120
              CF+  L N P +KISSV   VK R PFD +L +   G   RK + + V         
Sbjct: 61  ---CFQESLRNLPVKKISSVQRSVKNRTPFDSILYN---GGKKRKCERRRVQLEDEVSST 120

Query: 121 -------QNLAD----------KDGDYDRKGKRVMYQRPRTKIRRGRRAAVECGRLARGI 180
                  +N A           K G +  K KR+ + R     +   ++ +         
Sbjct: 121 TPSVGKFENKASGFDLLTAQSLKKGSFKVKSKRLSFHRSPYGFKNHYQSCLG-------- 180

Query: 181 CDSNEARKERESVALVERDEFIHDVINESNHIHLSERTWQGSESSGSVGENNYIDKGSST 240
             S E   +     LV          N+S    L + + + S S  SVG           
Sbjct: 181 LKSFEGSYDENDPLLVNS--------NDSGKA-LEDVSLRKSVSLSSVG----------- 240

Query: 241 IGQDTSNAEERGIIRNEASSIGLLEEALEEEKAARASLYMELEEERAAAATAADEAIAMI 300
            G         G+++    ++ + E+ L EE+AARASL +ELE+ER AAA+AADEA+ MI
Sbjct: 241 CGVGEGACSSPGMVQR---TVEMSEQVLGEERAARASLALELEKERNAAASAADEALGMI 300

Query: 301 IRLQNEKASVEMEARQYQRVIEEKFAYDEEEMNLLREILVKREIDYHVLEKEIEAYREMD 345
           +RLQ EKAS+EMEARQYQR+IEEK A+D EEM++L+EIL++RE + H LEKE++ YR+M 
Sbjct: 301 LRLQEEKASIEMEARQYQRMIEEKSAFDAEEMSILKEILLRREREKHFLEKEVDTYRQM- 326

BLAST of MS002456 vs. TAIR 10
Match: AT4G13160.1 (Protein of unknown function, DUF593 )

HSP 1 Score: 137.1 bits (344), Expect = 3.6e-32
Identity = 115/344 (33.43%), Postives = 164/344 (47.67%), Query Frame = 0

Query: 1   MSLHEIHPWTLSGLLGAFLDLALVYVLLCISATAFIPCKILKMIGLFLPCPCSGFYGRQN 60
           M   E +  T  G+L AF++LA  Y LLC+SA  FI  K+L    LF+PC      G QN
Sbjct: 1   MDYQESYRLTFYGILVAFMELAFAYCLLCVSAFVFITSKLL----LFMPC-----IGYQN 60

Query: 61  ANLCFRRLLFNWPKRKISSVLHFVKTRFPFDLLLVDDQIGNSNRKLQTKGVPQSQQNLAD 120
           ++LC ++LLF+WP R I  V                       +KL T   P        
Sbjct: 61  SDLCIQKLLFDWPFRIILRV-----------------------QKLATTNRPS------- 120

Query: 121 KDGDYDRKGKRVMYQRPRTKIRRGRRAAVECGRLARGICDSNEARKERESVALVERDEFI 180
                      V++ + +                        E  +E+E   +V++D+  
Sbjct: 121 -----------VLHHQEQ----------------------EQEQEQEQEEKTMVDKDK-- 180

Query: 181 HDVINESNHIHLSERTWQGSESSGSVGENNYIDKGSSTIGQDTSNAEERGIIRNEASSIG 240
                                                       N+E    +R       
Sbjct: 181 --------------------------------------------NSELMDRVR------- 219

Query: 241 LLEEALEEEKAARASLYMELEEERAAAATAADEAIAMIIRLQNEKASVEMEARQYQRVIE 300
           LLE A+E+EK A+A+L +ELE+ERAA+A+AADEA+AMI+RLQ +KAS+EME +QY+R+I+
Sbjct: 241 LLEVAVEQEKVAKAALMVELEQERAASASAADEAMAMILRLQADKASLEMEGKQYERMID 219

Query: 301 EKFAYDEEEMNLLREILVKREIDYHVLEKEIEAYREMDFSEEEQ 345
           EKFAYDEEEMN+L+EIL KRE + H LEKE+E Y+ +D  +E +
Sbjct: 301 EKFAYDEEEMNILKEILFKREREKHFLEKELETYKHIDDDQETE 219

BLAST of MS002456 vs. TAIR 10
Match: AT1G04890.1 (Protein of unknown function, DUF593 )

HSP 1 Score: 114.0 bits (284), Expect = 3.3e-25
Identity = 93/222 (41.89%), Postives = 138/222 (62.16%), Query Frame = 0

Query: 128 KGKRVMYQRPRTKIRRGRRAA-VECGRLARGICDSNEARKERESVALVERDEFIHDVINE 187
           KGKR + +R R  ++  RR+  V C  L R I  S+EA  E     L++  +      ++
Sbjct: 7   KGKRFVTRRSRNGLKNHRRSGRVNCASL-RSI-SSSEAFDETAKTNLMQPYDETCSNDDQ 66

Query: 188 SNHIHLSERT----WQGSESSGSVGENNYIDKGSSTIGQDTSNAEERGIIRN-EASSIGL 247
            +     E T    W   E + SV  N  ++  SS          E+  +RN E  S+  
Sbjct: 67  KDCGKAGEGTNLSHWPEFEKTVSV--NQQVNSNSSA---------EQSFVRNVEKRSVRD 126

Query: 248 LEEALEEEKAARASLYMELEEERAAAATAADEAIAMIIRLQNEKASVEMEARQYQRVIEE 307
           LEE L+EE+AARA++ +EL++ER+AAA+AADEA+AMI RLQ+EKA++EMEARQ+QR++EE
Sbjct: 127 LEELLKEERAARATVCVELDKERSAAASAADEAMAMIHRLQDEKAAIEMEARQFQRLVEE 186

Query: 308 KFAYDEEEMNLLREILVKREIDYHVLEKEIEAYREMDFSEEE 344
           +  +D EEM +L++IL++RE + H LEKE+EAYR++    EE
Sbjct: 187 RSTFDAEEMVILKDILIRREREKHFLEKEVEAYRQLLEETEE 215

BLAST of MS002456 vs. TAIR 10
Match: AT1G70750.1 (Protein of unknown function, DUF593 )

HSP 1 Score: 82.4 bits (202), Expect = 1.1e-15
Identity = 55/132 (41.67%), Postives = 85/132 (64.39%), Query Frame = 0

Query: 238 SIGLLEEALEEEKAARASLYMELEEERAAAATAADEAIAMIIRLQNEKASVEMEARQYQR 297
           ++  L+  L+EE+ A  +LY ELE ER A+A AA E +AMI RL  EKA+++MEA QYQR
Sbjct: 412 TVDKLKFELQEERKALHALYEELEVERNASAVAASETMAMINRLHEEKAAMQMEALQYQR 471

Query: 298 VIEEKFAYDEEEMNLLREILVKREIDYHVLEKEIEAYREMDFSEEEQSIRNWDFILDDHK 357
           ++EE+  +D+E + LL E++V RE +   LEKE+E YR+    EE ++      +    +
Sbjct: 472 MMEEQAEFDQEALQLLNELMVNREKENAELEKELEVYRKR--MEEYEAKEKMGMLRRRLR 531

Query: 358 EQSATTHYSNGD 370
           + S  ++ +NGD
Sbjct: 532 DSSVDSYRNNGD 541

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022150079.11.1e-25999.37uncharacterized protein LOC111018343 [Momordica charantia][more]
XP_038877576.12.0e-18167.77uncharacterized protein LOC120069830 isoform X1 [Benincasa hispida][more]
XP_038877577.18.0e-17566.41uncharacterized protein LOC120069830 isoform X2 [Benincasa hispida][more]
XP_022953644.11.9e-15563.91probable myosin-binding protein 6 [Cucurbita moschata][more]
KAG6575980.15.4e-15563.71Myosin-binding protein 3, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q9CAC41.5e-1441.67Myosin-binding protein 2 OS=Arabidopsis thaliana OX=3702 GN=MYOB2 PE=1 SV=1[more]
Q0WNW41.9e-1443.41Myosin-binding protein 3 OS=Arabidopsis thaliana OX=3702 GN=MYOB3 PE=1 SV=1[more]
F4HVS61.6e-1335.56Probable myosin-binding protein 6 OS=Arabidopsis thaliana OX=3702 GN=MYOB6 PE=2 ... [more]
Q9LMC81.8e-1248.94Probable myosin-binding protein 5 OS=Arabidopsis thaliana OX=3702 GN=MYOB5 PE=2 ... [more]
Q9FG146.9e-1235.52Myosin-binding protein 7 OS=Arabidopsis thaliana OX=3702 GN=MYOB7 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1D8Y85.1e-26099.37uncharacterized protein LOC111018343 OS=Momordica charantia OX=3673 GN=LOC111018... [more]
A0A6J1GQ959.0e-15663.91probable myosin-binding protein 6 OS=Cucurbita moschata OX=3662 GN=LOC111456111 ... [more]
A0A0A0K8C02.9e-15461.57GTD-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G339690... [more]
A0A1S3C7F21.4e-15363.64uncharacterized protein LOC103497547 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A5D3E5236.0e-15263.64Putative myosin-binding protein 5 isoform X2 OS=Cucumis melo var. makuwa OX=1194... [more]
Match NameE-valueIdentityDescription
AT4G13630.11.1e-4137.16Protein of unknown function, DUF593 [more]
AT4G13630.21.1e-4137.16Protein of unknown function, DUF593 [more]
AT4G13160.13.6e-3233.43Protein of unknown function, DUF593 [more]
AT1G04890.13.3e-2541.89Protein of unknown function, DUF593 [more]
AT1G70750.11.1e-1541.67Protein of unknown function, DUF593 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 271..298
NoneNo IPR availableCOILSCoilCoilcoord: 243..266
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 195..222
NoneNo IPR availablePANTHERPTHR31422:SF3BNAANNG28530D PROTEINcoord: 6..466
NoneNo IPR availablePANTHERPTHR31422BNAANNG28530D PROTEINcoord: 6..466
IPR007656GTD-binding domainPFAMPF04576Zein-bindingcoord: 242..331
e-value: 6.8E-31
score: 106.4
IPR007656GTD-binding domainPROSITEPS51775GTD_BINDINGcoord: 237..335
score: 19.774954

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS002456.1MS002456.1mRNA