MS002455 (gene) Bitter gourd (TR) v1

Overview
NameMS002455
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionPectin lyase-like superfamily protein
Locationscaffold318: 166859 .. 167271 (+)
RNA-Seq ExpressionMS002455
SyntenyMS002455
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AGTTTTAGGTTCAACTTCGTCAACAACTCAATAGTGAAGGGTATAACTTCATTGAACAGCAAGAATTTCCATGTCAATGTTATGAGTTGCAATAACGTTACCTTCGACCATTTCACAATTACTGCATCAGAGGACAGTCCAAACATTGATGGAATTCACATAGGTCGTTCCACTGGGATTAACATCATCAGCTCCAACATTGGAACTGGAGATGATTGTGTGTCTATAGGAGACACAAGCAAACAAATAACGGTCACAGATGTCACTTGTGGACCAGGACATGACATTAGCATAGGAAGTTTGGGAAAGTTCGAAAAGGTGAAGAGATCGTGGAAGGAGTTATAGTAAAAAAGTGCAAATTAATAAATACTTCCAACGGTGTTAGGATCAAAATATGGCCTGGTACTCCTGGC

mRNA sequence

AGTTTTAGGTTCAACTTCGTCAACAACTCAATAGTGAAGGGTATAACTTCATTGAACAGCAAGAATTTCCATGTCAATGTTATGAGTTGCAATAACGTTACCTTCGACCATTTCACAATTACTGCATCAGAGGACAGTCCAAACATTGATGGAATTCACATAGGTCGTTCCACTGGGATTAACATCATCAGCTCCAACATTGGAACTGGAGATGATTGTGTGTCTATAGGAGACACAAGCAAACAAATAACGGTCACAGATGTCACTTGTGGACCAGGACATGACATTAGCATAGGAAGTTTGGGAAAGTTCGAAGAAGAGATCGTGGAAGGAGTTATAGTAAAAAAGTGCAAATTAATAAATACTTCCAACGGTGTTAGGATCAAAATATGGCCTGGTACTCCTGGC

Coding sequence (CDS)

AGTTTTAGGTTCAACTTCGTCAACAACTCAATAGTGAAGGGTATAACTTCATTGAACAGCAAGAATTTCCATGTCAATGTTATGAGTTGCAATAACGTTACCTTCGACCATTTCACAATTACTGCATCAGAGGACAGTCCAAACATTGATGGAATTCACATAGGTCGTTCCACTGGGATTAACATCATCAGCTCCAACATTGGAACTGGAGATGATTGTGTGTCTATAGGAGACACAAGCAAACAAATAACGGTCACAGATGTCACTTGTGGACCAGGACATGACATTAGCATAGGAAGTTTGGGAAAGTTCGAAGAAGAGATCGTGGAAGGAGTTATAGTAAAAAAGTGCAAATTAATAAATACTTCCAACGGTGTTAGGATCAAAATATGGCCTGGTACTCCTGGC

Protein sequence

SFRFNFVNNSIVKGITSLNSKNFHVNVMSCNNVTFDHFTITASEDSPNIDGIHIGRSTGINIISSNIGTGDDCVSIGDTSKQITVTDVTCGPGHDISIGSLGKFEEEIVEGVIVKKCKLINTSNGVRIKIWPGTPG
Homology
BLAST of MS002455 vs. NCBI nr
Match: XP_022159064.1 (exopolygalacturonase-like [Momordica charantia])

HSP 1 Score: 254.2 bits (648), Expect = 6.3e-64
Identity = 127/137 (92.70%), Postives = 129/137 (94.16%), Query Frame = 0

Query: 1   SFRFNFVNNSIVKGITSLNSKNFHVNVMSCNNVTFDHFTITASEDSPNIDGIHIGRSTGI 60
           SFRFNFVN+SIVKGITSLNSKNFHVNVM CNNVTFDHFTITA EDSPN DGIHIGRSTGI
Sbjct: 157 SFRFNFVNHSIVKGITSLNSKNFHVNVMGCNNVTFDHFTITAPEDSPNTDGIHIGRSTGI 216

Query: 61  NIISSNIGTGDDCVSIGDTSKQITVTDVTCGPGHDISIGSLGKFE-EEIVEGVIVKKCKL 120
           NIISSNIGTGDDCVSIGDTSKQITVT+VTCGPGH ISIGSLGKFE EE VEGV VKKCKL
Sbjct: 217 NIISSNIGTGDDCVSIGDTSKQITVTBVTCGPGHGISIGSLGKFEGEENVEGVTVKKCKL 276

Query: 121 INTSNGVRIKIWPGTPG 137
           INTSNGVRIK WPGTPG
Sbjct: 277 INTSNGVRIKTWPGTPG 293

BLAST of MS002455 vs. NCBI nr
Match: XP_022143524.1 (polygalacturonase-like [Momordica charantia])

HSP 1 Score: 247.3 bits (630), Expect = 7.7e-62
Identity = 123/135 (91.11%), Postives = 126/135 (93.33%), Query Frame = 0

Query: 3   RFNFVNNSIVKGITSLNSKNFHVNVMSCNNVTFDHFTITASEDSPNIDGIHIGRSTGINI 62
           +FNFVNNSIVKGITSLNSKNFHVNVM CNNVTFDHFTI A EDSPN DGIHIGRS+GINI
Sbjct: 135 KFNFVNNSIVKGITSLNSKNFHVNVMGCNNVTFDHFTIIAPEDSPNTDGIHIGRSSGINI 194

Query: 63  ISSNIGTGDDCVSIGDTSKQITVTDVTCGPGHDISIGSLGKFE-EEIVEGVIVKKCKLIN 122
           ISSNIGT DDCVSIGDTSKQIT+TDVTCGPGH ISIGSLGKFE EEIVEGVIVKKCKLIN
Sbjct: 195 ISSNIGTRDDCVSIGDTSKQITITDVTCGPGHGISIGSLGKFEGEEIVEGVIVKKCKLIN 254

Query: 123 TSNGVRIKIWPGTPG 137
           TSNGV IK WPGTPG
Sbjct: 255 TSNGVTIKTWPGTPG 269

BLAST of MS002455 vs. NCBI nr
Match: XP_015881036.1 (exopolygalacturonase-like [Ziziphus jujuba])

HSP 1 Score: 199.1 bits (505), Expect = 2.4e-47
Identity = 93/133 (69.92%), Postives = 109/133 (81.95%), Query Frame = 0

Query: 1   SFRFNFVNNSIVKGITSLNSKNFHVNVMSCNNVTFDHFTITASEDSPNIDGIHIGRSTGI 60
           + R+NF+ NSI++GITSL+SK FH+NV+ CNNVTFDH T+TA EDSPN DGIHIGRSTGI
Sbjct: 143 NIRYNFITNSIIRGITSLDSKQFHMNVLGCNNVTFDHVTVTAPEDSPNTDGIHIGRSTGI 202

Query: 61  NIISSNIGTGDDCVSIGDTSKQITVTDVTCGPGHDISIGSLGKF-EEEIVEGVIVKKCKL 120
           NI  SNI TGDDC+S+GD +KQITV   TCGPGH IS+GSLGK+  EE VEGV ++ C L
Sbjct: 203 NITDSNIKTGDDCISLGDGAKQITVVKTTCGPGHGISVGSLGKYPNEEPVEGVFIRNCTL 262

Query: 121 INTSNGVRIKIWP 133
            NT NGVRIK WP
Sbjct: 263 TNTQNGVRIKTWP 275

BLAST of MS002455 vs. NCBI nr
Match: XP_022157826.1 (exopolygalacturonase-like [Momordica charantia])

HSP 1 Score: 198.4 bits (503), Expect = 4.1e-47
Identity = 94/137 (68.61%), Postives = 115/137 (83.94%), Query Frame = 0

Query: 1   SFRFNFVNNSIVKGITSLNSKNFHVNVMSCNNVTFDHFTITASEDSPNIDGIHIGRSTGI 60
           S +FNFV NSIVK ITSL+SKNFH+NV+ C N+TF H TITA  +SPN DGIHIG S GI
Sbjct: 146 SLKFNFVTNSIVKDITSLDSKNFHINVLGCKNLTFQHVTITAPGNSPNTDGIHIGCSEGI 205

Query: 61  NIISSNIGTGDDCVSIGDTSKQITVTDVTCGPGHDISIGSLGKF-EEEIVEGVIVKKCKL 120
           NI+++ I TGDDC+S+GDT+KQIT++DVTCGPGH I+IGSLGK+  E+ VEGV +KKCKL
Sbjct: 206 NILNTKIATGDDCISVGDTNKQITISDVTCGPGHGIAIGSLGKYTREQGVEGVTIKKCKL 265

Query: 121 INTSNGVRIKIWPGTPG 137
           I+T+NGVRIK WP + G
Sbjct: 266 IDTTNGVRIKTWPDSAG 282

BLAST of MS002455 vs. NCBI nr
Match: AFP50440.1 (polygalacturonase 11a, partial [Medicago truncatula])

HSP 1 Score: 196.4 bits (498), Expect = 1.6e-46
Identity = 95/137 (69.34%), Postives = 109/137 (79.56%), Query Frame = 0

Query: 1   SFRFNFVNNSIVKGITSLNSKNFHVNVMSCNNVTFDHFTITASEDSPNIDGIHIGRSTGI 60
           +F FNFVNNS+V G+TS +SKNFHV V  CNN+TFD FTITA  DSPN DGIH+G+STG+
Sbjct: 68  NFGFNFVNNSLVHGVTSKDSKNFHVMVFGCNNITFDSFTITAPGDSPNTDGIHMGKSTGV 127

Query: 61  NIISSNIGTGDDCVSIGDTSKQITVTDVTCGPGHDISIGSLGKF-EEEIVEGVIVKKCKL 120
            I+++NIGTGDDCVSIGD SKQITV  V CGPGH +SIGSLGKF  EE VEG+ +K C L
Sbjct: 128 KILNTNIGTGDDCVSIGDGSKQITVEGVKCGPGHGLSIGSLGKFTTEENVEGITIKNCTL 187

Query: 121 INTSNGVRIKIWPGTPG 137
             T NGVRIK WP  PG
Sbjct: 188 TATDNGVRIKTWPDAPG 204

BLAST of MS002455 vs. ExPASy Swiss-Prot
Match: Q40312 (Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 1.7e-48
Identity = 94/137 (68.61%), Postives = 108/137 (78.83%), Query Frame = 0

Query: 1   SFRFNFVNNSIVKGITSLNSKNFHVNVMSCNNVTFDHFTITASEDSPNIDGIHIGRSTGI 60
           +F FNFVNNSIV+G+TS +SKNFHV V  C N+TFD FTITA  DSPN DGIH+G+ST +
Sbjct: 149 NFGFNFVNNSIVRGVTSKDSKNFHVMVFGCKNITFDGFTITAPGDSPNTDGIHMGKSTDV 208

Query: 61  NIISSNIGTGDDCVSIGDTSKQITVTDVTCGPGHDISIGSLGKF-EEEIVEGVIVKKCKL 120
            I+++NIGTGDDCVSIGD SKQITV  V CGPGH +S+GSLGKF  EE VEG+ VK C L
Sbjct: 209 KILNTNIGTGDDCVSIGDGSKQITVQGVNCGPGHGLSVGSLGKFTTEENVEGITVKNCTL 268

Query: 121 INTSNGVRIKIWPGTPG 137
             T NGVRIK WP  PG
Sbjct: 269 TATDNGVRIKTWPDAPG 285

BLAST of MS002455 vs. ExPASy Swiss-Prot
Match: Q39766 (Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 2.0e-41
Identity = 77/137 (56.20%), Postives = 105/137 (76.64%), Query Frame = 0

Query: 1   SFRFNFVNNSIVKGITSLNSKNFHVNVMSCNNVTFDHFTITASEDSPNIDGIHIGRSTGI 60
           + RF+FV N++++ ITS +SK FH+NV +C N+T +   I A ++SPN DGIH+G+S G+
Sbjct: 151 NIRFDFVTNALIQDITSKDSKLFHINVFACKNITLERLKIEAPDESPNTDGIHMGKSEGV 210

Query: 61  NIISSNIGTGDDCVSIGDTSKQITVTDVTCGPGHDISIGSLGKFE-EEIVEGVIVKKCKL 120
           NII+S+I TGDDC+SIGD +K + + ++TCGPGH ISIGSLGKF+ EE VEG+ +  C +
Sbjct: 211 NIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNCTI 270

Query: 121 INTSNGVRIKIWPGTPG 137
            NTSNG RIK WPG  G
Sbjct: 271 TNTSNGARIKTWPGEHG 287

BLAST of MS002455 vs. ExPASy Swiss-Prot
Match: Q39786 (Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 4.6e-41
Identity = 76/137 (55.47%), Postives = 105/137 (76.64%), Query Frame = 0

Query: 1   SFRFNFVNNSIVKGITSLNSKNFHVNVMSCNNVTFDHFTITASEDSPNIDGIHIGRSTGI 60
           + RF+F+ N++++ ITS +SK FH+NV +C N+T +   I A ++SPN DGIH+G+S G+
Sbjct: 151 NIRFDFLTNALIQDITSKDSKLFHINVFACKNITLERLKIEAPDESPNTDGIHMGKSEGV 210

Query: 61  NIISSNIGTGDDCVSIGDTSKQITVTDVTCGPGHDISIGSLGKFE-EEIVEGVIVKKCKL 120
           NII+S+I TGDDC+SIGD +K + + ++TCGPGH ISIGSLGKF+ EE VEG+ +  C +
Sbjct: 211 NIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNCTI 270

Query: 121 INTSNGVRIKIWPGTPG 137
            NTSNG RIK WPG  G
Sbjct: 271 TNTSNGARIKTWPGEHG 287

BLAST of MS002455 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 3.9e-40
Identity = 77/133 (57.89%), Postives = 103/133 (77.44%), Query Frame = 0

Query: 1   SFRFNFVNNSIVKGITSLNSKNFHVNVMSCNNVTFDHFTITASEDSPNIDGIHIGRSTGI 60
           + R   V NSI++ +T+L+SKNFHVNV+ C N+TF+ F I+A+E S N DGIHIGRS G+
Sbjct: 109 NLRLYAVTNSILRDVTTLDSKNFHVNVIGCKNLTFERFKISAAETSINTDGIHIGRSDGV 168

Query: 61  NIISSNIGTGDDCVSIGDTSKQITVTDVTCGPGHDISIGSLGKFE-EEIVEGVIVKKCKL 120
           NII++ I TGDDC+S+GD SK I +T++TCGPGH IS+GSLG+++ EE V G+ VK C +
Sbjct: 169 NIINTEIKTGDDCISLGDGSKNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCTI 228

Query: 121 INTSNGVRIKIWP 133
             + NGVRIK WP
Sbjct: 229 TGSQNGVRIKTWP 241

BLAST of MS002455 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 1.8e-37
Identity = 72/136 (52.94%), Postives = 98/136 (72.06%), Query Frame = 0

Query: 1   SFRFNFVNNSIVKGITSLNSKNFHVNVMSCNNVTFDHFTITASEDSPNIDGIHIGRSTGI 60
           + RF+F+ +++V+ ITSLNSK FH+NV+ C ++TF H T+TA   S N DGIH+G S G+
Sbjct: 130 NLRFDFLKHAMVRDITSLNSKMFHINVLECEDITFQHVTVTAPGTSINTDGIHVGISKGV 189

Query: 61  NIISSNIGTGDDCVSIGDTSKQITVTDVTCGPGHDISIGSLGKF-EEEIVEGVIVKKCKL 120
            I ++ I TGDDC+SIG  S+ +T+T V CGPGH ISIGSLG++  E+ V G+ VK C  
Sbjct: 190 TITNTKIATGDDCISIGPGSQNVTITQVNCGPGHGISIGSLGRYNNEKEVRGITVKGCTF 249

Query: 121 INTSNGVRIKIWPGTP 136
             T NGVR+K WP +P
Sbjct: 250 SGTMNGVRVKTWPNSP 265

BLAST of MS002455 vs. ExPASy TrEMBL
Match: A0A6J1E198 (exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111025503 PE=3 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 1.8e-64
Identity = 128/137 (93.43%), Postives = 129/137 (94.16%), Query Frame = 0

Query: 1   SFRFNFVNNSIVKGITSLNSKNFHVNVMSCNNVTFDHFTITASEDSPNIDGIHIGRSTGI 60
           SFRFNFVN+SIVKGITSLNSKNFHVNVM CNNVTFDHFTITA EDSPN DGIHIGRSTGI
Sbjct: 157 SFRFNFVNHSIVKGITSLNSKNFHVNVMGCNNVTFDHFTITAPEDSPNTDGIHIGRSTGI 216

Query: 61  NIISSNIGTGDDCVSIGDTSKQITVTDVTCGPGHDISIGSLGKFE-EEIVEGVIVKKCKL 120
           NIISSNIGTGDDCVSIGDTSKQITVTDVTCGPGH ISIGSLGKFE EE VEGV VKKCKL
Sbjct: 217 NIISSNIGTGDDCVSIGDTSKQITVTDVTCGPGHGISIGSLGKFEGEENVEGVTVKKCKL 276

Query: 121 INTSNGVRIKIWPGTPG 137
           INTSNGVRIK WPGTPG
Sbjct: 277 INTSNGVRIKTWPGTPG 293

BLAST of MS002455 vs. ExPASy TrEMBL
Match: A0A6J1CPK0 (polygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111013395 PE=3 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 3.7e-62
Identity = 123/135 (91.11%), Postives = 126/135 (93.33%), Query Frame = 0

Query: 3   RFNFVNNSIVKGITSLNSKNFHVNVMSCNNVTFDHFTITASEDSPNIDGIHIGRSTGINI 62
           +FNFVNNSIVKGITSLNSKNFHVNVM CNNVTFDHFTI A EDSPN DGIHIGRS+GINI
Sbjct: 135 KFNFVNNSIVKGITSLNSKNFHVNVMGCNNVTFDHFTIIAPEDSPNTDGIHIGRSSGINI 194

Query: 63  ISSNIGTGDDCVSIGDTSKQITVTDVTCGPGHDISIGSLGKFE-EEIVEGVIVKKCKLIN 122
           ISSNIGT DDCVSIGDTSKQIT+TDVTCGPGH ISIGSLGKFE EEIVEGVIVKKCKLIN
Sbjct: 195 ISSNIGTRDDCVSIGDTSKQITITDVTCGPGHGISIGSLGKFEGEEIVEGVIVKKCKLIN 254

Query: 123 TSNGVRIKIWPGTPG 137
           TSNGV IK WPGTPG
Sbjct: 255 TSNGVTIKTWPGTPG 269

BLAST of MS002455 vs. ExPASy TrEMBL
Match: A0A6P4A5K0 (exopolygalacturonase-like OS=Ziziphus jujuba OX=326968 GN=LOC107416994 PE=3 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 1.2e-47
Identity = 93/133 (69.92%), Postives = 109/133 (81.95%), Query Frame = 0

Query: 1   SFRFNFVNNSIVKGITSLNSKNFHVNVMSCNNVTFDHFTITASEDSPNIDGIHIGRSTGI 60
           + R+NF+ NSI++GITSL+SK FH+NV+ CNNVTFDH T+TA EDSPN DGIHIGRSTGI
Sbjct: 143 NIRYNFITNSIIRGITSLDSKQFHMNVLGCNNVTFDHVTVTAPEDSPNTDGIHIGRSTGI 202

Query: 61  NIISSNIGTGDDCVSIGDTSKQITVTDVTCGPGHDISIGSLGKF-EEEIVEGVIVKKCKL 120
           NI  SNI TGDDC+S+GD +KQITV   TCGPGH IS+GSLGK+  EE VEGV ++ C L
Sbjct: 203 NITDSNIKTGDDCISLGDGAKQITVVKTTCGPGHGISVGSLGKYPNEEPVEGVFIRNCTL 262

Query: 121 INTSNGVRIKIWP 133
            NT NGVRIK WP
Sbjct: 263 TNTQNGVRIKTWP 275

BLAST of MS002455 vs. ExPASy TrEMBL
Match: A0A6J1DZB6 (exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111024445 PE=3 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 2.0e-47
Identity = 94/137 (68.61%), Postives = 115/137 (83.94%), Query Frame = 0

Query: 1   SFRFNFVNNSIVKGITSLNSKNFHVNVMSCNNVTFDHFTITASEDSPNIDGIHIGRSTGI 60
           S +FNFV NSIVK ITSL+SKNFH+NV+ C N+TF H TITA  +SPN DGIHIG S GI
Sbjct: 146 SLKFNFVTNSIVKDITSLDSKNFHINVLGCKNLTFQHVTITAPGNSPNTDGIHIGCSEGI 205

Query: 61  NIISSNIGTGDDCVSIGDTSKQITVTDVTCGPGHDISIGSLGKF-EEEIVEGVIVKKCKL 120
           NI+++ I TGDDC+S+GDT+KQIT++DVTCGPGH I+IGSLGK+  E+ VEGV +KKCKL
Sbjct: 206 NILNTKIATGDDCISVGDTNKQITISDVTCGPGHGIAIGSLGKYTREQGVEGVTIKKCKL 265

Query: 121 INTSNGVRIKIWPGTPG 137
           I+T+NGVRIK WP + G
Sbjct: 266 IDTTNGVRIKTWPDSAG 282

BLAST of MS002455 vs. ExPASy TrEMBL
Match: G7ISY5 (Polygalacturonase 11c, putative OS=Medicago truncatula OX=3880 GN=MTR_2g058840 PE=3 SV=2)

HSP 1 Score: 196.4 bits (498), Expect = 7.5e-47
Identity = 95/137 (69.34%), Postives = 109/137 (79.56%), Query Frame = 0

Query: 1   SFRFNFVNNSIVKGITSLNSKNFHVNVMSCNNVTFDHFTITASEDSPNIDGIHIGRSTGI 60
           +F FNFVNNS+V G+TS +SKNFHV V  CNN+TFD FTITA  DSPN DGIH+G+STG+
Sbjct: 147 NFGFNFVNNSLVHGVTSKDSKNFHVMVFGCNNITFDSFTITAPGDSPNTDGIHMGKSTGV 206

Query: 61  NIISSNIGTGDDCVSIGDTSKQITVTDVTCGPGHDISIGSLGKF-EEEIVEGVIVKKCKL 120
            I+++NIGTGDDCVSIGD SKQITV  V CGPGH +SIGSLGKF  EE VEG+ +K C L
Sbjct: 207 KILNTNIGTGDDCVSIGDGSKQITVEGVKCGPGHGLSIGSLGKFTTEENVEGITIKNCTL 266

Query: 121 INTSNGVRIKIWPGTPG 137
             T NGVRIK WP  PG
Sbjct: 267 TATDNGVRIKTWPDAPG 283

BLAST of MS002455 vs. TAIR 10
Match: AT3G07850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 155.6 bits (392), Expect = 2.8e-38
Identity = 75/136 (55.15%), Postives = 98/136 (72.06%), Query Frame = 0

Query: 1   SFRFNFVNNSIVKGITSLNSKNFHVNVMSCNNVTFDHFTITASEDSPNIDGIHIGRSTGI 60
           + RF  + NS +  ITS NSK FH+N+++C N+T     I A  +S N DGIHIGRS G+
Sbjct: 191 NIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLSDIGIDAPPESLNTDGIHIGRSNGV 250

Query: 61  NIISSNIGTGDDCVSIGDTSKQITVTDVTCGPGHDISIGSLGKF-EEEIVEGVIVKKCKL 120
           N+I + I TGDDCVSIGD ++ + V +V CGPGH ISIGSLG++  E+ V+GV V+KC +
Sbjct: 251 NLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKCLI 310

Query: 121 INTSNGVRIKIWPGTP 136
            NT NGVRIK WPG+P
Sbjct: 311 KNTDNGVRIKTWPGSP 326

BLAST of MS002455 vs. TAIR 10
Match: AT3G14040.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 155.2 bits (391), Expect = 3.7e-38
Identity = 75/136 (55.15%), Postives = 98/136 (72.06%), Query Frame = 0

Query: 1   SFRFNFVNNSIVKGITSLNSKNFHVNVMSCNNVTFDHFTITASEDSPNIDGIHIGRSTGI 60
           + RF  + NS +  ITS NSK FH+N+++C N+T     I A  +S N DGIHIGRS G+
Sbjct: 192 NIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLTDIGIDAPPESLNTDGIHIGRSNGV 251

Query: 61  NIISSNIGTGDDCVSIGDTSKQITVTDVTCGPGHDISIGSLGKF-EEEIVEGVIVKKCKL 120
           N+I + I TGDDCVSIGD ++ + V +V CGPGH ISIGSLG++  E+ V+GV V+KC +
Sbjct: 252 NLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKCLI 311

Query: 121 INTSNGVRIKIWPGTP 136
            NT NGVRIK WPG+P
Sbjct: 312 KNTDNGVRIKTWPGSP 327

BLAST of MS002455 vs. TAIR 10
Match: AT5G48140.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 154.8 bits (390), Expect = 4.8e-38
Identity = 76/133 (57.14%), Postives = 97/133 (72.93%), Query Frame = 0

Query: 1   SFRFNFVNNSIVKGITSLNSKNFHVNVMSCNNVTFDHFTITASEDSPNIDGIHIGRSTGI 60
           S RF+FV ++ ++GITSL++K+FH+NV+   NVTF+   I A  +SPN DGIH+GRS GI
Sbjct: 140 SIRFDFVTDAKIRGITSLDAKHFHINVIGAKNVTFEDVKIIAPAESPNTDGIHVGRSDGI 199

Query: 61  NIISSNIGTGDDCVSIGDTSKQITVTDVTCGPGHDISIGSLGKF-EEEIVEGVIVKKCKL 120
            II+S I TGDDCVS+GD  K + V  VTCGPGH ISIGSLG++  EE V G+ +  C L
Sbjct: 200 KIINSFISTGDDCVSVGDGMKNLLVERVTCGPGHGISIGSLGRYSHEENVSGIKIINCTL 259

Query: 121 INTSNGVRIKIWP 133
             T NG+RIK WP
Sbjct: 260 QETDNGLRIKTWP 272

BLAST of MS002455 vs. TAIR 10
Match: AT3G07820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 152.1 bits (383), Expect = 3.1e-37
Identity = 71/133 (53.38%), Postives = 97/133 (72.93%), Query Frame = 0

Query: 1   SFRFNFVNNSIVKGITSLNSKNFHVNVMSCNNVTFDHFTITASEDSPNIDGIHIGRSTGI 60
           S RF+F+ NS ++ I+S+++KNFH+NV+   N+T ++  I A EDSPN DGIH+GRS G+
Sbjct: 140 SIRFDFILNSEIRDISSIDAKNFHINVLGAKNMTMNNIKIVAPEDSPNTDGIHLGRSDGV 199

Query: 61  NIISSNIGTGDDCVSIGDTSKQITVTDVTCGPGHDISIGSLGKF-EEEIVEGVIVKKCKL 120
            I++S I TGDDC+S+GD  K + V  VTCGPGH IS+GSLG++  E+ V G+ V  C L
Sbjct: 200 KILNSFISTGDDCISVGDGMKNLHVEKVTCGPGHGISVGSLGRYGHEQDVSGIKVINCTL 259

Query: 121 INTSNGVRIKIWP 133
             T NG+RIK WP
Sbjct: 260 QETDNGLRIKTWP 272

BLAST of MS002455 vs. TAIR 10
Match: AT3G07840.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 152.1 bits (383), Expect = 3.1e-37
Identity = 69/133 (51.88%), Postives = 97/133 (72.93%), Query Frame = 0

Query: 1   SFRFNFVNNSIVKGITSLNSKNFHVNVMSCNNVTFDHFTITASEDSPNIDGIHIGRSTGI 60
           S RF+FV N+ ++ I+S+++KNFH+NV+   N+TFD+  + A  +SPN DGIH+GRS G+
Sbjct: 141 SIRFDFVENAEIRDISSIDAKNFHINVLGAKNMTFDNVKVIAPAESPNTDGIHLGRSEGV 200

Query: 61  NIISSNIGTGDDCVSIGDTSKQITVTDVTCGPGHDISIGSLGKF-EEEIVEGVIVKKCKL 120
            I++S I TGDDC+S+GD  K + V +V CGPGH IS+GSLG++  E+ V G+ V  C L
Sbjct: 201 KILNSKIATGDDCISVGDGMKNLHVENVMCGPGHGISVGSLGRYVHEQDVTGITVVNCTL 260

Query: 121 INTSNGVRIKIWP 133
             T NG+RIK WP
Sbjct: 261 QGTDNGLRIKTWP 273

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022159064.16.3e-6492.70exopolygalacturonase-like [Momordica charantia][more]
XP_022143524.17.7e-6291.11polygalacturonase-like [Momordica charantia][more]
XP_015881036.12.4e-4769.92exopolygalacturonase-like [Ziziphus jujuba][more]
XP_022157826.14.1e-4768.61exopolygalacturonase-like [Momordica charantia][more]
AFP50440.11.6e-4669.34polygalacturonase 11a, partial [Medicago truncatula][more]
Match NameE-valueIdentityDescription
Q403121.7e-4868.61Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1[more]
Q397662.0e-4156.20Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1[more]
Q397864.6e-4155.47Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1[more]
P245483.9e-4057.89Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1[more]
Q6H9K01.8e-3752.94Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A6J1E1981.8e-6493.43exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111025503 PE=3 SV... [more]
A0A6J1CPK03.7e-6291.11polygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111013395 PE=3 SV=1[more]
A0A6P4A5K01.2e-4769.92exopolygalacturonase-like OS=Ziziphus jujuba OX=326968 GN=LOC107416994 PE=3 SV=1[more]
A0A6J1DZB62.0e-4768.61exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111024445 PE=3 SV... [more]
G7ISY57.5e-4769.34Polygalacturonase 11c, putative OS=Medicago truncatula OX=3880 GN=MTR_2g058840 P... [more]
Match NameE-valueIdentityDescription
AT3G07850.12.8e-3855.15Pectin lyase-like superfamily protein [more]
AT3G14040.13.7e-3855.15Pectin lyase-like superfamily protein [more]
AT5G48140.14.8e-3857.14Pectin lyase-like superfamily protein [more]
AT3G07820.13.1e-3753.38Pectin lyase-like superfamily protein [more]
AT3G07840.13.1e-3751.88Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 1..136
e-value: 1.2E-45
score: 157.8
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 2..134
e-value: 2.0E-46
score: 158.5
NoneNo IPR availablePANTHERPTHR31375:SF248POLYGALACTURONASEcoord: 2..134
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 2..134
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 2..135

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS002455.1MS002455.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity