MS002084 (gene) Bitter gourd (TR) v1

Overview


Name	MS002084
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	EF hand calcium-binding protein family
Location	scaffold30: 2281352 .. 2281783 (+)
RNA-Seq Expression	MS002084
Synteny	MS002084

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AATCAATTACGCGACATATTTGCACGATTCGACATGGATTCCGACGGAAGCCTGACGATTCTTGAGCTGGCGGCGTTGCTCCGGTCGCTAGGTCTAAAGCCCTCCGGCGACCAAATCCACGTTCTCGTCGCGAACATGGATGCGAACGGGAATGGATCTGTAGAGTTCGACGAATTGGTGACCGCGATTATGCCGGATTTCAACGAGGAGGTAATGGTAAATCAGACGCAGCTTCTGGAGGTTTTCCGTTCCTTCGATCGAGACGGGAATGGATATATAACCGCGGCGGAGCTGGCGGGATCGATGGCGAAGATGGGACAGCCATTGACGTACAGAGAGCTAACGGAAATGATGAAGGAAGCCGATACGGACGGAGATGGCGTAATTAGCTTCAACGAATTTGCATCTGTAATGGCTAAATCCGCATCTGAA

mRNA sequence

AATCAATTACGCGACATATTTGCACGATTCGACATGGATTCCGACGGAAGCCTGACGATTCTTGAGCTGGCGGCGTTGCTCCGGTCGCTAGGTCTAAAGCCCTCCGGCGACCAAATCCACGTTCTCGTCGCGAACATGGATGCGAACGGGAATGGATCTGTAGAGTTCGACGAATTGGTGACCGCGATTATGCCGGATTTCAACGAGGAGGTAATGGTAAATCAGACGCAGCTTCTGGAGGTTTTCCGTTCCTTCGATCGAGACGGGAATGGATATATAACCGCGGCGGAGCTGGCGGGATCGATGGCGAAGATGGGACAGCCATTGACGTACAGAGAGCTAACGGAAATGATGAAGGAAGCCGATACGGACGGAGATGGCGTAATTAGCTTCAACGAATTTGCATCTGTAATGGCTAAATCCGCATCTGAA

Coding sequence (CDS)

AATCAATTACGCGACATATTTGCACGATTCGACATGGATTCCGACGGAAGCCTGACGATTCTTGAGCTGGCGGCGTTGCTCCGGTCGCTAGGTCTAAAGCCCTCCGGCGACCAAATCCACGTTCTCGTCGCGAACATGGATGCGAACGGGAATGGATCTGTAGAGTTCGACGAATTGGTGACCGCGATTATGCCGGATTTCAACGAGGAGGTAATGGTAAATCAGACGCAGCTTCTGGAGGTTTTCCGTTCCTTCGATCGAGACGGGAATGGATATATAACCGCGGCGGAGCTGGCGGGATCGATGGCGAAGATGGGACAGCCATTGACGTACAGAGAGCTAACGGAAATGATGAAGGAAGCCGATACGGACGGAGATGGCGTAATTAGCTTCAACGAATTTGCATCTGTAATGGCTAAATCCGCATCTGAA

Protein sequence

NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISFNEFASVMAKSASE

Homology

[Close]

BLAST of MS002084 vs. NCBI nr
Match: XP_022135250.1 (probable calcium-binding protein CML15 [Momordica charantia])

HSP 1 Score: 280.0 bits (715), Expect = 1.1e-71
Identity = 144/144 (100.00%), Postives = 144/144 (100.00%), Query Frame = 0


Query: 1   NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELV 60
           NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELV
Sbjct: 10  NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELV 69

Query: 61  TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 120
           TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE
Sbjct: 70  TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 129

Query: 121 ADTDGDGVISFNEFASVMAKSASE 145
           ADTDGDGVISFNEFASVMAKSASE
Sbjct: 130 ADTDGDGVISFNEFASVMAKSASE 153

[Close]

BLAST of MS002084 vs. NCBI nr
Match: KAG6589571.1 (putative calcium-binding protein CML15, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 276.6 bits (706), Expect = 1.3e-70
Identity = 142/144 (98.61%), Postives = 143/144 (99.31%), Query Frame = 0


Query: 1   NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELV 60
           NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVL+ANMDANGNGSVEFDELV
Sbjct: 76  NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDANGNGSVEFDELV 135

Query: 61  TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 120
           TAI PDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE
Sbjct: 136 TAIRPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 195

Query: 121 ADTDGDGVISFNEFASVMAKSASE 145
           ADTDGDGVISFNEFASVMAKSASE
Sbjct: 196 ADTDGDGVISFNEFASVMAKSASE 219

[Close]

BLAST of MS002084 vs. NCBI nr
Match: XP_022921807.1 (probable calcium-binding protein CML15 [Cucurbita moschata] >XP_023516651.1 probable calcium-binding protein CML15 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 276.6 bits (706), Expect = 1.3e-70
Identity = 142/144 (98.61%), Postives = 143/144 (99.31%), Query Frame = 0


Query: 1   NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELV 60
           NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVL+ANMDANGNGSVEFDELV
Sbjct: 10  NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDANGNGSVEFDELV 69

Query: 61  TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 120
           TAI PDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE
Sbjct: 70  TAIRPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 129

Query: 121 ADTDGDGVISFNEFASVMAKSASE 145
           ADTDGDGVISFNEFASVMAKSASE
Sbjct: 130 ADTDGDGVISFNEFASVMAKSASE 153

[Close]

BLAST of MS002084 vs. NCBI nr
Match: XP_004138337.2 (probable calcium-binding protein CML15 [Cucumis sativus] >XP_031741380.1 probable calcium-binding protein CML15 [Cucumis sativus])

HSP 1 Score: 275.4 bits (703), Expect = 2.8e-70
Identity = 140/144 (97.22%), Postives = 144/144 (100.00%), Query Frame = 0


Query: 1   NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELV 60
           NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVL+ANMD+NGNGSVEFDELV
Sbjct: 10  NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELV 69

Query: 61  TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 120
           TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE
Sbjct: 70  TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 129

Query: 121 ADTDGDGVISFNEFASVMAKSASE 145
           ADTDGDGVISFNEFASVMAKSA++
Sbjct: 130 ADTDGDGVISFNEFASVMAKSAAD 153

[Close]

BLAST of MS002084 vs. NCBI nr
Match: XP_038880166.1 (probable calcium-binding protein CML15 [Benincasa hispida])

HSP 1 Score: 275.4 bits (703), Expect = 2.8e-70
Identity = 140/144 (97.22%), Postives = 144/144 (100.00%), Query Frame = 0


Query: 1   NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELV 60
           NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVL+ANMD+NGNGSVEFDELV
Sbjct: 10  NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELV 69

Query: 61  TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 120
           TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE
Sbjct: 70  TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 129

Query: 121 ADTDGDGVISFNEFASVMAKSASE 145
           ADTDGDGVISFNEFASVMAKSA++
Sbjct: 130 ADTDGDGVISFNEFASVMAKSAAD 153

[Close]

BLAST of MS002084 vs. ExPASy Swiss-Prot
Match: Q9FZ75 (Probable calcium-binding protein CML15 OS=Arabidopsis thaliana OX=3702 GN=CML15 PE=2 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 1.9e-61
Identity = 116/141 (82.27%), Postives = 133/141 (94.33%), Query Frame = 0


Query: 2   QLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELVT 61
           QL+DIF RFDMD+DGSLTILELAALLRSLGLKPSGDQIHVL+A+MD+NGNG VEFDELV 
Sbjct: 7   QLKDIFDRFDMDADGSLTILELAALLRSLGLKPSGDQIHVLLASMDSNGNGFVEFDELVG 66

Query: 62  AIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEA 121
            I+PD NEEV++N  QLLE+F+SFDRDGNG+I+AAELAG+MAKMGQPLTY+ELTEM+KEA
Sbjct: 67  TILPDLNEEVLINSEQLLEIFKSFDRDGNGFISAAELAGAMAKMGQPLTYKELTEMIKEA 126

Query: 122 DTDGDGVISFNEFASVMAKSA 143
           DT+GDGVISF EFAS+MAKSA
Sbjct: 127 DTNGDGVISFGEFASIMAKSA 147

[Close]

BLAST of MS002084 vs. ExPASy Swiss-Prot
Match: Q9LI84 (Probable calcium-binding protein CML16 OS=Arabidopsis thaliana OX=3702 GN=CML16 PE=2 SV=2)

HSP 1 Score: 228.8 bits (582), Expect = 3.9e-59
Identity = 114/143 (79.72%), Postives = 129/143 (90.21%), Query Frame = 0


Query: 2   QLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELVT 61
           QL+DIFARFDMD DGSLT LELAALLRSLG+KP GDQI +L+  +D NGNGSVEFDELV 
Sbjct: 12  QLKDIFARFDMDKDGSLTQLELAALLRSLGIKPRGDQISLLLNQIDRNGNGSVEFDELVV 71

Query: 62  AIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEA 121
           AI+PD NEEV++NQ QL+EVFRSFDRDGNG ITAAELAGSMAKMG PLTYRELTEMM EA
Sbjct: 72  AILPDINEEVLINQEQLMEVFRSFDRDGNGSITAAELAGSMAKMGHPLTYRELTEMMTEA 131

Query: 122 DTDGDGVISFNEFASVMAKSASE 145
           D++GDGVISFNEF+ +MAKSA++
Sbjct: 132 DSNGDGVISFNEFSHIMAKSAAD 154

[Close]

BLAST of MS002084 vs. ExPASy Swiss-Prot
Match: Q6L5F4 (Probable calcium-binding protein CML14 OS=Oryza sativa subsp. japonica OX=39947 GN=CML14 PE=2 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 3.6e-52
Identity = 103/142 (72.54%), Postives = 123/142 (86.62%), Query Frame = 0


Query: 2   QLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELVT 61
           QLR++F RFDM+ DGSLT LELAALLRSLGL+P+GD++H L+A MDANGNGSVEFDEL  
Sbjct: 25  QLRELFRRFDMNGDGSLTQLELAALLRSLGLRPTGDEVHALLAGMDANGNGSVEFDELAA 84

Query: 62  AIMPDFNEEV-MVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 121
           AI P    +  +V+Q QLLEVFR+FDRDGNG+I+AAELA SMA++GQPLT+ ELT MM++
Sbjct: 85  AIAPVLTTQTHLVDQAQLLEVFRAFDRDGNGFISAAELARSMARLGQPLTFEELTRMMRD 144

Query: 122 ADTDGDGVISFNEFASVMAKSA 143
           ADTDGDGVISF EFA+VMAKSA
Sbjct: 145 ADTDGDGVISFKEFAAVMAKSA 166

[Close]

BLAST of MS002084 vs. ExPASy Swiss-Prot
Match: Q5ZD81 (Probable calcium-binding protein CML12 OS=Oryza sativa subsp. japonica OX=39947 GN=CML12 PE=2 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 1.9e-45
Identity = 94/146 (64.38%), Postives = 118/146 (80.82%), Query Frame = 0


Query: 2   QLRDIFARFDMDSDGSLTILELAALLRSLGLKP-SGDQIHVLVANMDANGNGSVEFDELV 61
           QL +IF RFD+D DGSLT LELAALLRSLGL+P +GD+IH L+A +DA+GNG+VEFDEL 
Sbjct: 95  QLHEIFLRFDLDGDGSLTKLELAALLRSLGLRPAAGDEIHALIAAIDADGNGTVEFDELA 154

Query: 62  TA----IMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTE 121
           ++    I+      V V+Q +L E FR+FDRDGNG+I+AAELA SMA+MG P+ Y ELT+
Sbjct: 155 SSLADLILGPCRPSVAVDQAELAEAFRAFDRDGNGFISAAELARSMARMGHPICYAELTD 214

Query: 122 MMKEADTDGDGVISFNEFASVMAKSA 143
           MM+EADTDGDG+ISF EF ++MAKSA
Sbjct: 215 MMREADTDGDGLISFEEFTAIMAKSA 240

[Close]

BLAST of MS002084 vs. ExPASy Swiss-Prot
Match: Q9M8U1 (Probable calcium-binding protein CML18 OS=Arabidopsis thaliana OX=3702 GN=CML18 PE=1 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 3.6e-36
Identity = 81/141 (57.45%), Postives = 94/141 (66.67%), Query Frame = 0


Query: 2   QLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELVT 61
           +LR+IF  FD + DGSLT LEL +LLRSLGLKPS DQ+  L+   D N NG VEF E V 
Sbjct: 20  ELREIFRSFDQNKDGSLTELELGSLLRSLGLKPSQDQLDTLIQKADRNNNGLVEFSEFVA 79

Query: 62  AIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEA 121
            + PD   +      QL  +FR FDRDGNGYITAAELA SMAK+G  LT  ELT M+KEA
Sbjct: 80  LVEPDL-VKCPYTDDQLKAIFRMFDRDGNGYITAAELAHSMAKLGHALTAEELTGMIKEA 139

Query: 122 DTDGDGVISFNEFASVMAKSA 143
           D DGDG I F EF   +  +A
Sbjct: 140 DRDGDGCIDFQEFVQAITSAA 159

[Close]

BLAST of MS002084 vs. ExPASy TrEMBL
Match: A0A6J1C0M6 (probable calcium-binding protein CML15 OS=Momordica charantia OX=3673 GN=LOC111007259 PE=4 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 5.5e-72
Identity = 144/144 (100.00%), Postives = 144/144 (100.00%), Query Frame = 0


Query: 1   NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELV 60
           NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELV
Sbjct: 10  NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELV 69

Query: 61  TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 120
           TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE
Sbjct: 70  TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 129

Query: 121 ADTDGDGVISFNEFASVMAKSASE 145
           ADTDGDGVISFNEFASVMAKSASE
Sbjct: 130 ADTDGDGVISFNEFASVMAKSASE 153

[Close]

BLAST of MS002084 vs. ExPASy TrEMBL
Match: A0A6J1E1G5 (probable calcium-binding protein CML15 OS=Cucurbita moschata OX=3662 GN=LOC111429950 PE=4 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 6.1e-71
Identity = 142/144 (98.61%), Postives = 143/144 (99.31%), Query Frame = 0


Query: 1   NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELV 60
           NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVL+ANMDANGNGSVEFDELV
Sbjct: 10  NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDANGNGSVEFDELV 69

Query: 61  TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 120
           TAI PDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE
Sbjct: 70  TAIRPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 129

Query: 121 ADTDGDGVISFNEFASVMAKSASE 145
           ADTDGDGVISFNEFASVMAKSASE
Sbjct: 130 ADTDGDGVISFNEFASVMAKSASE 153

[Close]

BLAST of MS002084 vs. ExPASy TrEMBL
Match: A0A0A0LUS7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G015780 PE=4 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 1.3e-70
Identity = 140/144 (97.22%), Postives = 144/144 (100.00%), Query Frame = 0


Query: 1   NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELV 60
           NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVL+ANMD+NGNGSVEFDELV
Sbjct: 10  NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELV 69

Query: 61  TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 120
           TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE
Sbjct: 70  TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 129

Query: 121 ADTDGDGVISFNEFASVMAKSASE 145
           ADTDGDGVISFNEFASVMAKSA++
Sbjct: 130 ADTDGDGVISFNEFASVMAKSAAD 153

[Close]

BLAST of MS002084 vs. ExPASy TrEMBL
Match: A0A6J1JGZ7 (probable calcium-binding protein CML15 OS=Cucurbita maxima OX=3661 GN=LOC111485022 PE=4 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 3.0e-70
Identity = 141/144 (97.92%), Postives = 142/144 (98.61%), Query Frame = 0


Query: 1   NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELV 60
           NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVL+ANMDANGNGSVEFDELV
Sbjct: 10  NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDANGNGSVEFDELV 69

Query: 61  TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 120
           TAI PDFNEEV VNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE
Sbjct: 70  TAIRPDFNEEVTVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 129

Query: 121 ADTDGDGVISFNEFASVMAKSASE 145
           ADTDGDGVISFNEFASVMAKSASE
Sbjct: 130 ADTDGDGVISFNEFASVMAKSASE 153

[Close]

BLAST of MS002084 vs. ExPASy TrEMBL
Match: A0A5D3DXV7 (Putative calcium-binding protein CML15 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00110 PE=4 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 3.9e-70
Identity = 139/144 (96.53%), Postives = 144/144 (100.00%), Query Frame = 0


Query: 1   NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELV 60
           NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVL+ANMD+NGNGSVEFDELV
Sbjct: 10  NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELV 69

Query: 61  TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 120
           TAIMPDFNEEVMVNQT+LLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE
Sbjct: 70  TAIMPDFNEEVMVNQTRLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 129

Query: 121 ADTDGDGVISFNEFASVMAKSASE 145
           ADTDGDGVISFNEFASVMAKSA++
Sbjct: 130 ADTDGDGVISFNEFASVMAKSAAD 153

[Close]

BLAST of MS002084 vs. TAIR 10
Match: AT1G18530.1 (EF hand calcium-binding protein family )

HSP 1 Score: 236.5 bits (602), Expect = 1.3e-62
Identity = 116/141 (82.27%), Postives = 133/141 (94.33%), Query Frame = 0


Query: 2   QLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELVT 61
           QL+DIF RFDMD+DGSLTILELAALLRSLGLKPSGDQIHVL+A+MD+NGNG VEFDELV 
Sbjct: 7   QLKDIFDRFDMDADGSLTILELAALLRSLGLKPSGDQIHVLLASMDSNGNGFVEFDELVG 66

Query: 62  AIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEA 121
            I+PD NEEV++N  QLLE+F+SFDRDGNG+I+AAELAG+MAKMGQPLTY+ELTEM+KEA
Sbjct: 67  TILPDLNEEVLINSEQLLEIFKSFDRDGNGFISAAELAGAMAKMGQPLTYKELTEMIKEA 126

Query: 122 DTDGDGVISFNEFASVMAKSA 143
           DT+GDGVISF EFAS+MAKSA
Sbjct: 127 DTNGDGVISFGEFASIMAKSA 147

[Close]

BLAST of MS002084 vs. TAIR 10
Match: AT3G25600.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 228.8 bits (582), Expect = 2.8e-60
Identity = 114/143 (79.72%), Postives = 129/143 (90.21%), Query Frame = 0


Query: 2   QLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELVT 61
           QL+DIFARFDMD DGSLT LELAALLRSLG+KP GDQI +L+  +D NGNGSVEFDELV 
Sbjct: 12  QLKDIFARFDMDKDGSLTQLELAALLRSLGIKPRGDQISLLLNQIDRNGNGSVEFDELVV 71

Query: 62  AIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEA 121
           AI+PD NEEV++NQ QL+EVFRSFDRDGNG ITAAELAGSMAKMG PLTYRELTEMM EA
Sbjct: 72  AILPDINEEVLINQEQLMEVFRSFDRDGNGSITAAELAGSMAKMGHPLTYRELTEMMTEA 131

Query: 122 DTDGDGVISFNEFASVMAKSASE 145
           D++GDGVISFNEF+ +MAKSA++
Sbjct: 132 DSNGDGVISFNEFSHIMAKSAAD 154

[Close]

BLAST of MS002084 vs. TAIR 10
Match: AT3G03000.1 (EF hand calcium-binding protein family )

HSP 1 Score: 152.5 bits (384), Expect = 2.5e-37
Identity = 81/141 (57.45%), Postives = 94/141 (66.67%), Query Frame = 0


Query: 2   QLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELVT 61
           +LR+IF  FD + DGSLT LEL +LLRSLGLKPS DQ+  L+   D N NG VEF E V 
Sbjct: 20  ELREIFRSFDQNKDGSLTELELGSLLRSLGLKPSQDQLDTLIQKADRNNNGLVEFSEFVA 79

Query: 62  AIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEA 121
            + PD   +      QL  +FR FDRDGNGYITAAELA SMAK+G  LT  ELT M+KEA
Sbjct: 80  LVEPDL-VKCPYTDDQLKAIFRMFDRDGNGYITAAELAHSMAKLGHALTAEELTGMIKEA 139

Query: 122 DTDGDGVISFNEFASVMAKSA 143
           D DGDG I F EF   +  +A
Sbjct: 140 DRDGDGCIDFQEFVQAITSAA 159

[Close]

BLAST of MS002084 vs. TAIR 10
Match: AT1G32250.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 146.7 bits (369), Expect = 1.4e-35
Identity = 78/146 (53.42%), Postives = 96/146 (65.75%), Query Frame = 0


Query: 1   NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELV 60
           N+LR+IF  FD + DGSLT LEL +LLR+LG+KPS DQ   L+   D   NG VEF E V
Sbjct: 15  NELREIFRSFDRNKDGSLTQLELGSLLRALGVKPSPDQFETLIDKADTKSNGLVEFPEFV 74

Query: 61  TAIMPDF----NEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTE 120
             + P+            + QLL +FR FD DGNG+ITAAELA SMAK+G  LT  ELT 
Sbjct: 75  ALVSPELLSPAKRTTPYTEEQLLRLFRIFDTDGNGFITAAELAHSMAKLGHALTVAELTG 134

Query: 121 MMKEADTDGDGVISFNEFASVMAKSA 143
           M+KEAD+DGDG I+F EFA  +  +A
Sbjct: 135 MIKEADSDGDGRINFQEFAKAINSAA 160

[Close]

BLAST of MS002084 vs. TAIR 10
Match: AT2G41110.1 (calmodulin 2 )

HSP 1 Score: 113.6 bits (283), Expect = 1.3e-25
Identity = 55/138 (39.86%), Postives = 88/138 (63.77%), Query Frame = 0


Query: 1   NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELV 60
           ++ ++ F+ FD D DG +T  EL  ++RSLG  P+  ++  ++  +DA+GNG+++F E +
Sbjct: 11  SEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFL 70

Query: 61  TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 120
             +     +    ++ +L E FR FD+D NG+I+AAEL   M  +G+ LT  E+ EM+KE
Sbjct: 71  NLMARKMKD--TDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDEMIKE 130

Query: 121 ADTDGDGVISFNEFASVM 139
           AD DGDG I++ EF  VM
Sbjct: 131 ADVDGDGQINYEEFVKVM 146

The following BLAST results are available for this feature:

BLAST of MS002084 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135250.1	1.1e-71	100.00	probable calcium-binding protein CML15 [Momordica charantia]	[more]
KAG6589571.1	1.3e-70	98.61	putative calcium-binding protein CML15, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_022921807.1	1.3e-70	98.61	probable calcium-binding protein CML15 [Cucurbita moschata] >XP_023516651.1 prob...	[more]
XP_004138337.2	2.8e-70	97.22	probable calcium-binding protein CML15 [Cucumis sativus] >XP_031741380.1 probabl...	[more]
XP_038880166.1	2.8e-70	97.22	probable calcium-binding protein CML15 [Benincasa hispida]	[more]

BLAST of MS002084 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FZ75	1.9e-61	82.27	Probable calcium-binding protein CML15 OS=Arabidopsis thaliana OX=3702 GN=CML15 ...	[more]
Q9LI84	3.9e-59	79.72	Probable calcium-binding protein CML16 OS=Arabidopsis thaliana OX=3702 GN=CML16 ...	[more]
Q6L5F4	3.6e-52	72.54	Probable calcium-binding protein CML14 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q5ZD81	1.9e-45	64.38	Probable calcium-binding protein CML12 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9M8U1	3.6e-36	57.45	Probable calcium-binding protein CML18 OS=Arabidopsis thaliana OX=3702 GN=CML18 ...	[more]

BLAST of MS002084 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C0M6	5.5e-72	100.00	probable calcium-binding protein CML15 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1E1G5	6.1e-71	98.61	probable calcium-binding protein CML15 OS=Cucurbita moschata OX=3662 GN=LOC11142...	[more]
A0A0A0LUS7	1.3e-70	97.22	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G015780 PE=4 SV=1	[more]
A0A6J1JGZ7	3.0e-70	97.92	probable calcium-binding protein CML15 OS=Cucurbita maxima OX=3661 GN=LOC1114850...	[more]
A0A5D3DXV7	3.9e-70	96.53	Putative calcium-binding protein CML15 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]

BLAST of MS002084 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G18530.1	1.3e-62	82.27	EF hand calcium-binding protein family	[more]
AT3G25600.1	2.8e-60	79.72	Calcium-binding EF-hand family protein	[more]
AT3G03000.1	2.5e-37	57.45	EF hand calcium-binding protein family	[more]
AT1G32250.1	1.4e-35	53.42	Calcium-binding EF-hand family protein	[more]
AT2G41110.1	1.3e-25	39.86	calmodulin 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 2..30 e-value: 0.046 score: 22.9 coord: 77..105 e-value: 5.7E-4 score: 29.2 coord: 38..66 e-value: 3.1 score: 13.8 coord: 113..141 e-value: 8.4E-8 score: 41.9
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 2..63 e-value: 8.3E-11 score: 42.2 coord: 76..138 e-value: 2.8E-15 score: 56.5
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 109..144 score: 14.318138
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 34..69 score: 9.018011
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 1..33 score: 12.951263
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 73..108 score: 14.039183
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 2..64 e-value: 1.18373E-12 score: 57.1725
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 77..139 e-value: 8.35533E-19 score: 72.9657
None	No IPR available	GENE3D	1.10.238.10		coord: 1..69 e-value: 4.9E-17 score: 64.5
None	No IPR available	GENE3D	1.10.238.10		coord: 70..144 e-value: 6.9E-24 score: 85.9
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 1..144
None	No IPR available	PANTHER	PTHR23050:SF351	CALCIUM-BINDING PROTEIN CML15-RELATED	coord: 1..144
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 47..59
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 86..98
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 11..23
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 122..134
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 2..139

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS002084.1	MS002084.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding