MS001635 (gene) Bitter gourd (TR) v1

Overview
NameMS001635
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptioncalcium-dependent protein kinase 24-like
Locationscaffold377: 125674 .. 130272 (-)
RNA-Seq ExpressionMS001635
SyntenyMS001635
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAAGCTGCGTTTCCGTGCAAGCCAGAGCAGCGTCCTTCGCCAAGAGGAGCAGAGACGTCAGGCCTCTTCCGGATCATGCGCTCATCGAATCGGTTCGCAAGTCCTCCGTCCGCCTCGCGGCTTCGATTCTCTCCGATGAGTCCACCGGCGACAACATTTTCGAGAAATACCGCTTCGGGAAGGAGCTCGGCCGCGGGGAGTTCGGGATTACGCACCAGTGCTTTGACATTGAGACCGGCGATACCTTCGCCTGCAAGACCATTTCCAAGAGCAAGTTGAGGTCGGAGATCAACGTCGAGGATGTGCGGAGAGAGGTGGCGATTATGCGAAATTTGCCCAAGCATCCCAACATTGTCGCCTTCAAGGAGGCCTTTGAGGATAATGAAGCGGTTTATTTCGTTATGGAGCTTTGCGAGGGCGGCGAGCTCTTCGATAGGATTGTCTCCAAGGGGCACTACACTGAGCGTGCGGCTGCCGACGTAACCAAGACCATTATCGAGATTTGCAAGGTAATATCAATTTTACCCTCCTCTTTAATCACAGGGAAATTACGGAATATGATATTTTCTGGAAGTAGACTGGAATTTGTTTGTGTAATTACTTGCTGATTTCTTCAAAATGGTGATGTTTACGCGTTATTTGCATTTGTGACAAGTCATTTCTGCCTCATGTTTGCCATGGAAATATCTGTTTAATGAGATAATAGCCTCTGAATTTGGTAGCAGGGCAATGGTGGCATGATTGAGGTTTAATGGAATGATATTGGGGAAGTTGTGTCCTTAGAAATGGCATTTTTCCAATGTATAAATTGTCCTTCATTCTCTATACAGCTCAATTTCATAGAGAAATTCTAGTCCTCTATATTGTTTTCTAATCTCTAAACTGGTTAAAGAATACTAATGTTTCAACTGAAGATTAAGGTTCTTTAGAACAACACATATGTGAAGGATTGATCGTATTAATGATGTTTTTGAAATATGCAAGGTTGCATAACTGTTTTTACTTTTTCAGCTTCAATGTTATTTTCTTTAAGCATTTTATTGTTCTGTCCCAGGTATGCCATGAAAATGGAGTAATCCATAGGGATTTGAAACCCGAGAACTTCTTGTTCGCAGATGAAAGTGAAAATTCTCGACTGAAGGCAATTGATTTTGGTCTTTCCATATTTTTTGAACCTGGTATACTTGCAGTTCTTAACTACTTGCCCGTAATTCTGATTGCATGAGCCAAACTTCTCCTCTCATCTTATTGGCTTGGGGAGCTACTCTATATTTGATTAAGAACTTGTTTCTGAACTTTCTCGATCTCTTTTGATAGATCAGAGATTCAGTGAAATTGTTGGAAGTCCGTATTACATGGCACCAGAGGTCCTGAGACGCAGTTATGGACCTGAAATTGATGTTTGGAGCGCTGGGGTCATTCTCTACATTTTACTTTGTGGAGTTCCTCCTTTCTGGGCAGGTGAGTCTCTCCTTGAGTGGCATCTTGTAGGTTGTTTATGAATTATGAGGTCCGTTCTTCCCAAAAAAGTACAAGTCCTGTTTCGTTCTTTGAACTTTTATACTTATTTATTTTGGTTTCAAACCTTCAAAATGTTTTATTTTTTTAGTACCTCAATTTTCAGATGTTTTGTTTTTGGTCCCTAAACTTTACAAAGATAAAATAAAAACTTTAATCTTTACCATTAACTTTTTATTGATCATTTAACAAGAATCTAAATATATTTCAATTTGACCTCTTACGTATATTTTCGAGCACCTGTTTATGTAGAATTTTACTCATTAGGTAAATGAACAAAAGATGTGCTTATGTTAGGTTGTTGAATAATTTATTTAAAAATCAAGGGCAATGACTAAAATAGTTTTCCATGTAAATTTCAAGGAGTAAAGCAAAATGTTTGAAAGTTTAAGAACCAAAATAAAATAAATATAAAAAATCAGGAAACAAAACTTGATTTATATATAAGAAAATCAAGTGGCTAAGCATTTTTCTAGGAATGTTAATGATGCATCGTGGTTTATAACTAGTGCAGAATCTGAGGAAGGCATTGCTCATGCTATTGTTCGGGGTGATATAGATTTTGAGAGGGACCCTTGGCCCAAGGTCTCCAAGGAAGCAAAAGAACTTGTGATGGGGATGCTTGATCCAAATCCTTATAGTCGGATGACTGTTGAAGAAGTACTTGGTATGGACTCTAATTCCCAATCCATTTCAATAATATTTTTCTTTGCTTTGTGTTTTCAATTCCTAAGCAGAGTACCTCTAGGTTTTAAATAAGATTCAATAGAAAGGATCACAAATGCAGGCATGAGCCGTTAAAGAGTTGTCAGATTTTGTAGCTAAGTTGAAGTATTTTTGTAGTTTGGATTTTAGCAATTAAGAAGCTTGTTTTTTGGTGTTTTGTTAGTTGAATTTTGTGTGGTTTATTGATTGGTTGGTAGGTATTTTTCTAATTAGATTTTGAGAAGGTATTAATTTCACCACTCATTCGAGTTGAAAATTAAAGGTTAAAATTACTCACTAAATCAAGTATAAAGGTAAAAATTATTTTTGCCCTCATTTATTATTATACAAGAATCAAATACCACCCATATGTTCTTGTGTAGGCAGAAATTAAAGAAATTGGTCTGTTTCAACTGAGATTAATGGTTTGCACAAGCTGGAGCTTATTCAAAATTTACTGCTTGCCTCATTCTTCTTGACGATTTTCACCATGTGTTTCTTTTTCTTTGAGTAAACATGGAGCTACATGCAAAACCTAAAAATGAAGAGAAGAATTAAAACATAAATCATAAATTAAATTATCTGATCATCTTGTTTTATTACATGAAGAACATCCTTGGATACAAAACAAAAACCAGGCTCGTAATGTCTCCCTGGGAGAAAATGTCGGCTTAAGGATTAAGCAATTCACTGTGATGAATAAGTTCAAGAAGAAAGTACTTAGAGTAAGTTTGTCACACACCCTCTTACTCGAACATACATTAGTCAATTAAATTCTATCTTCAGTACTTGCCAATTGATGAATATTCTTTTCTGGGTCCAGGTTGTGGCTGACCACTTGTCAGATGAGCAAATGGATGGGATCAGACGAATGTTCCATATGATGGACACTGATAAAAATGGAGACTTGACTTTTGAAGAGTTGAAGAATGGTCTGCATATGATTGGACATTCTCTTCCTGACCCTGATGTACGGATGTTGATGGATGCTGTAAGTTTCTTCACACCCAAAAGTTTGTTGGATGGATATTTTGAGAGAAGACGCTCTTATACACTTTTACCCTTTACTTTTTTCCTCACGTGCATTAAATGTAGGGACAGCCTTGTTTATAAACAAAAGTCTGTTGGAGTTGGAGTCTGAGTAAAAAAATATCTTTCTAACCAGTTTTTCAGTCCAAATATCTTAGATATTTTTTTTGGGAGGCTAACCAAATAGAAAGAAATCCTTGAGTTCTTCAAAGTCCAAAAAGTATATTGAAGTCTAACATATTTTTGGAGTAATTTAAGATATTTGTTTAGAACCTTCTTGAACAAATCATTTTCATTCACCATGCCTTCTATTTTCTCTATAAAATAGTCAGTTGGTTCTCCCACGTGGTTAGGATCCTCATTCATGAGAAATCCAAATAAGAGGATTGAACCATTTCTTCCCACAGTTTTTTCTATTTGATGAATGATGAACTGTTATTAAAAGATGAAGTATTATCATATAACGAATATTTGAGGTCAAAATTACGAGTCTAATATTCTAACGAGGCTCTTCCTCTCCATGAATAAGGCGGACATGGATGGAAATGGAACACTAAGCTGCGAGGAGTTCGCAACAATGTCCATCCACCTCAGAAAGCTGAGCACTGATGAGCTCCTGACTCAAGCTTTCAGTTACTTTGACAAAGACAAAAACGGGTACATTGAATATGATGAACTGAGAGAAGCCTTAATGGATGACAATGAGAAGGTGATCCAAGAAATCATTTCTGATGTTGACTCAGATAAGGTATCTCTCTAAAATGGCTATTTTTCACTTCATTTCATTTCCTTTATGAGTATTAGATTCTTTTGGGAAAAAGTGAAAAGTACTTTTAGCTCATTACTATTAGAAAAAATTTCATAATGTTTTAGTGTTGTATGAATAGGATCTATTCCATAGGTTATACTACTGTGTCATCGAGTTCTATAGTGAAACACTTGCTATATAGGACTTTTTATTTGTAGAACCTCTCAAACCATTTTTTTATCATAAAATTTTTTTAAGAGTTTTTAATTGATGAAAAGTACTCTTGACCGCTCTTTAAAATTATAACCAAATTCAATTTTTACAAAAATATTTTGTCATCTATAAGTAATTTAAAAATCGTGTCAAAACAACTGGTTACATAGTTAAAAGCACTTATAGAAAAAATGTTTCATCTAAAAACACTACTCTCGAAAGTCATTTCAAACTTACTCTTTGGTTGCTTGCTGGGTAGGATGGTCGAATCAGTTACAATGAGTTCAAAGCCATGCTAACCACAGGGTTGGATTGGAAAATGGCTTCGCGGCAGTACTCGAGAGCAATGCTTCATGCGCTTAGCCGCAAATTGTTCAAAGAC

mRNA sequence

ATGGGAAGCTGCGTTTCCGTGCAAGCCAGAGCAGCGTCCTTCGCCAAGAGGAGCAGAGACGTCAGGCCTCTTCCGGATCATGCGCTCATCGAATCGGTTCGCAAGTCCTCCGTCCGCCTCGCGGCTTCGATTCTCTCCGATGAGTCCACCGGCGACAACATTTTCGAGAAATACCGCTTCGGGAAGGAGCTCGGCCGCGGGGAGTTCGGGATTACGCACCAGTGCTTTGACATTGAGACCGGCGATACCTTCGCCTGCAAGACCATTTCCAAGAGCAAGTTGAGGTCGGAGATCAACGTCGAGGATGTGCGGAGAGAGGTGGCGATTATGCGAAATTTGCCCAAGCATCCCAACATTGTCGCCTTCAAGGAGGCCTTTGAGGATAATGAAGCGGTTTATTTCGTTATGGAGCTTTGCGAGGGCGGCGAGCTCTTCGATAGGATTGTCTCCAAGGGGCACTACACTGAGCGTGCGGCTGCCGACGTAACCAAGACCATTATCGAGATTTGCAAGGTATGCCATGAAAATGGAGTAATCCATAGGGATTTGAAACCCGAGAACTTCTTGTTCGCAGATGAAAGTGAAAATTCTCGACTGAAGGCAATTGATTTTGGTCTTTCCATATTTTTTGAACCTGATCAGAGATTCAGTGAAATTGTTGGAAGTCCGTATTACATGGCACCAGAGGTCCTGAGACGCAGTTATGGACCTGAAATTGATGTTTGGAGCGCTGGGGTCATTCTCTACATTTTACTTTGTGGAGTTCCTCCTTTCTGGGCAGAATCTGAGGAAGGCATTGCTCATGCTATTGTTCGGGGTGATATAGATTTTGAGAGGGACCCTTGGCCCAAGGTCTCCAAGGAAGCAAAAGAACTTGTGATGGGGATGCTTGATCCAAATCCTTATAGTCGGATGACTGTTGAAGAAGTACTTGAACATCCTTGGATACAAAACAAAAACCAGGCTCGTAATGTCTCCCTGGGAGAAAATGTCGGCTTAAGGATTAAGCAATTCACTGTGATGAATAAGTTCAAGAAGAAAGTACTTAGAGTTGTGGCTGACCACTTGTCAGATGAGCAAATGGATGGGATCAGACGAATGTTCCATATGATGGACACTGATAAAAATGGAGACTTGACTTTTGAAGAGTTGAAGAATGGTCTGCATATGATTGGACATTCTCTTCCTGACCCTGATGTACGGATGTTGATGGATGCTGCGGACATGGATGGAAATGGAACACTAAGCTGCGAGGAGTTCGCAACAATGTCCATCCACCTCAGAAAGCTGAGCACTGATGAGCTCCTGACTCAAGCTTTCAGTTACTTTGACAAAGACAAAAACGGGTACATTGAATATGATGAACTGAGAGAAGCCTTAATGGATGACAATGAGAAGGTGATCCAAGAAATCATTTCTGATGTTGACTCAGATAAGGATGGTCGAATCAGTTACAATGAGTTCAAAGCCATGCTAACCACAGGGTTGGATTGGAAAATGGCTTCGCGGCAGTACTCGAGAGCAATGCTTCATGCGCTTAGCCGCAAATTGTTCAAAGAC

Coding sequence (CDS)

ATGGGAAGCTGCGTTTCCGTGCAAGCCAGAGCAGCGTCCTTCGCCAAGAGGAGCAGAGACGTCAGGCCTCTTCCGGATCATGCGCTCATCGAATCGGTTCGCAAGTCCTCCGTCCGCCTCGCGGCTTCGATTCTCTCCGATGAGTCCACCGGCGACAACATTTTCGAGAAATACCGCTTCGGGAAGGAGCTCGGCCGCGGGGAGTTCGGGATTACGCACCAGTGCTTTGACATTGAGACCGGCGATACCTTCGCCTGCAAGACCATTTCCAAGAGCAAGTTGAGGTCGGAGATCAACGTCGAGGATGTGCGGAGAGAGGTGGCGATTATGCGAAATTTGCCCAAGCATCCCAACATTGTCGCCTTCAAGGAGGCCTTTGAGGATAATGAAGCGGTTTATTTCGTTATGGAGCTTTGCGAGGGCGGCGAGCTCTTCGATAGGATTGTCTCCAAGGGGCACTACACTGAGCGTGCGGCTGCCGACGTAACCAAGACCATTATCGAGATTTGCAAGGTATGCCATGAAAATGGAGTAATCCATAGGGATTTGAAACCCGAGAACTTCTTGTTCGCAGATGAAAGTGAAAATTCTCGACTGAAGGCAATTGATTTTGGTCTTTCCATATTTTTTGAACCTGATCAGAGATTCAGTGAAATTGTTGGAAGTCCGTATTACATGGCACCAGAGGTCCTGAGACGCAGTTATGGACCTGAAATTGATGTTTGGAGCGCTGGGGTCATTCTCTACATTTTACTTTGTGGAGTTCCTCCTTTCTGGGCAGAATCTGAGGAAGGCATTGCTCATGCTATTGTTCGGGGTGATATAGATTTTGAGAGGGACCCTTGGCCCAAGGTCTCCAAGGAAGCAAAAGAACTTGTGATGGGGATGCTTGATCCAAATCCTTATAGTCGGATGACTGTTGAAGAAGTACTTGAACATCCTTGGATACAAAACAAAAACCAGGCTCGTAATGTCTCCCTGGGAGAAAATGTCGGCTTAAGGATTAAGCAATTCACTGTGATGAATAAGTTCAAGAAGAAAGTACTTAGAGTTGTGGCTGACCACTTGTCAGATGAGCAAATGGATGGGATCAGACGAATGTTCCATATGATGGACACTGATAAAAATGGAGACTTGACTTTTGAAGAGTTGAAGAATGGTCTGCATATGATTGGACATTCTCTTCCTGACCCTGATGTACGGATGTTGATGGATGCTGCGGACATGGATGGAAATGGAACACTAAGCTGCGAGGAGTTCGCAACAATGTCCATCCACCTCAGAAAGCTGAGCACTGATGAGCTCCTGACTCAAGCTTTCAGTTACTTTGACAAAGACAAAAACGGGTACATTGAATATGATGAACTGAGAGAAGCCTTAATGGATGACAATGAGAAGGTGATCCAAGAAATCATTTCTGATGTTGACTCAGATAAGGATGGTCGAATCAGTTACAATGAGTTCAAAGCCATGCTAACCACAGGGTTGGATTGGAAAATGGCTTCGCGGCAGTACTCGAGAGCAATGCTTCATGCGCTTAGCCGCAAATTGTTCAAAGAC

Protein sequence

MGSCVSVQARAASFAKRSRDVRPLPDHALIESVRKSSVRLAASILSDESTGDNIFEKYRFGKELGRGEFGITHQCFDIETGDTFACKTISKSKLRSEINVEDVRREVAIMRNLPKHPNIVAFKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICKVCHENGVIHRDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVLRRSYGPEIDVWSAGVILYILLCGVPPFWAESEEGIAHAIVRGDIDFERDPWPKVSKEAKELVMGMLDPNPYSRMTVEEVLEHPWIQNKNQARNVSLGENVGLRIKQFTVMNKFKKKVLRVVADHLSDEQMDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHSLPDPDVRMLMDAADMDGNGTLSCEEFATMSIHLRKLSTDELLTQAFSYFDKDKNGYIEYDELREALMDDNEKVIQEIISDVDSDKDGRISYNEFKAMLTTGLDWKMASRQYSRAMLHALSRKLFKD
Homology
BLAST of MS001635 vs. NCBI nr
Match: XP_022145110.1 (calcium-dependent protein kinase 24-like [Momordica charantia])

HSP 1 Score: 1045.4 bits (2702), Expect = 1.6e-301
Identity = 519/520 (99.81%), Postives = 520/520 (100.00%), Query Frame = 0

Query: 1   MGSCVSVQARAASFAKRSRDVRPLPDHALIESVRKSSVRLAASILSDESTGDNIFEKYRF 60
           MGSCVSVQARAASFAKRSRDVRPLPDHALIESVRKSSVRLAASILSDESTGDNIFEKYRF
Sbjct: 1   MGSCVSVQARAASFAKRSRDVRPLPDHALIESVRKSSVRLAASILSDESTGDNIFEKYRF 60

Query: 61  GKELGRGEFGITHQCFDIETGDTFACKTISKSKLRSEINVEDVRREVAIMRNLPKHPNIV 120
           GKELGRGEFGITHQCFDIETGDTFACKTISKSKLRSEINVEDVRREVAIMRNLPKHPNIV
Sbjct: 61  GKELGRGEFGITHQCFDIETGDTFACKTISKSKLRSEINVEDVRREVAIMRNLPKHPNIV 120

Query: 121 AFKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICKVCHENGVIH 180
           AFKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICKVCHENGVIH
Sbjct: 121 AFKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICKVCHENGVIH 180

Query: 181 RDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVLRRSYGPEID 240
           RDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVLRRSYGPEID
Sbjct: 181 RDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVLRRSYGPEID 240

Query: 241 VWSAGVILYILLCGVPPFWAESEEGIAHAIVRGDIDFERDPWPKVSKEAKELVMGMLDPN 300
           VWSAGVILYILLCGVPPFWAESEEGIAHAIVRGDIDFERDPWPKVSKEAKELVMGMLDPN
Sbjct: 241 VWSAGVILYILLCGVPPFWAESEEGIAHAIVRGDIDFERDPWPKVSKEAKELVMGMLDPN 300

Query: 301 PYSRMTVEEVLEHPWIQNKNQARNVSLGENVGLRIKQFTVMNKFKKKVLRVVADHLSDEQ 360
           PYSRM+VEEVLEHPWIQNKNQARNVSLGENVGLRIKQFTVMNKFKKKVLRVVADHLSDEQ
Sbjct: 301 PYSRMSVEEVLEHPWIQNKNQARNVSLGENVGLRIKQFTVMNKFKKKVLRVVADHLSDEQ 360

Query: 361 MDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHSLPDPDVRMLMDAADMDGNGTLSCEEF 420
           MDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHSLPDPDVRMLMDAADMDGNGTLSCEEF
Sbjct: 361 MDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHSLPDPDVRMLMDAADMDGNGTLSCEEF 420

Query: 421 ATMSIHLRKLSTDELLTQAFSYFDKDKNGYIEYDELREALMDDNEKVIQEIISDVDSDKD 480
           ATMSIHLRKLSTDELLTQAFSYFDKDKNGYIEYDELREALMDDNEKVIQEIISDVDSDKD
Sbjct: 421 ATMSIHLRKLSTDELLTQAFSYFDKDKNGYIEYDELREALMDDNEKVIQEIISDVDSDKD 480

Query: 481 GRISYNEFKAMLTTGLDWKMASRQYSRAMLHALSRKLFKD 521
           GRISYNEFKAMLTTGLDWKMASRQYSRAMLHALSRKLFKD
Sbjct: 481 GRISYNEFKAMLTTGLDWKMASRQYSRAMLHALSRKLFKD 520

BLAST of MS001635 vs. NCBI nr
Match: XP_038877045.1 (calcium-dependent protein kinase 24 [Benincasa hispida])

HSP 1 Score: 1009.2 bits (2608), Expect = 1.3e-290
Identity = 497/521 (95.39%), Postives = 516/521 (99.04%), Query Frame = 0

Query: 1   MGSCVSVQARAA-SFAKRSRDVRPLPDHALIESVRKSSVRLAASILSDESTGDNIFEKYR 60
           MGSCVSVQAR+A SF+KR+RDVRPLPDHALIESVRKSSVR AASILS+ESTGDNIFEKYR
Sbjct: 1   MGSCVSVQARSAGSFSKRTRDVRPLPDHALIESVRKSSVRHAASILSNESTGDNIFEKYR 60

Query: 61  FGKELGRGEFGITHQCFDIETGDTFACKTISKSKLRSEINVEDVRREVAIMRNLPKHPNI 120
           FGKELGRGEFGITHQCF+IETG+TFACKTISKSKLRSEINVEDVRREVAIMRNLPKHPNI
Sbjct: 61  FGKELGRGEFGITHQCFEIETGETFACKTISKSKLRSEINVEDVRREVAIMRNLPKHPNI 120

Query: 121 VAFKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICKVCHENGVI 180
           V FKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICKVCHENGVI
Sbjct: 121 VTFKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICKVCHENGVI 180

Query: 181 HRDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVLRRSYGPEI 240
           HRDLKPENFLFADESENS+LKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVLRRSYGPEI
Sbjct: 181 HRDLKPENFLFADESENSQLKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVLRRSYGPEI 240

Query: 241 DVWSAGVILYILLCGVPPFWAESEEGIAHAIVRGDIDFERDPWPKVSKEAKELVMGMLDP 300
           DVWSAGVILYILLCGVPPFWAESEEGIAHAIVRGDIDFERDPWPKVSKEAKELV+GMLDP
Sbjct: 241 DVWSAGVILYILLCGVPPFWAESEEGIAHAIVRGDIDFERDPWPKVSKEAKELVIGMLDP 300

Query: 301 NPYSRMTVEEVLEHPWIQNKNQARNVSLGENVGLRIKQFTVMNKFKKKVLRVVADHLSDE 360
           NPYSRMTVEEVLEHPWIQNKNQARNVSLGENVG+RIKQFT+MNKFKKKVLRVVADHLSDE
Sbjct: 301 NPYSRMTVEEVLEHPWIQNKNQARNVSLGENVGIRIKQFTLMNKFKKKVLRVVADHLSDE 360

Query: 361 QMDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHSLPDPDVRMLMDAADMDGNGTLSCEE 420
           QMDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGH+LPDPDVRMLMDAADMDGNGTLSCEE
Sbjct: 361 QMDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHALPDPDVRMLMDAADMDGNGTLSCEE 420

Query: 421 FATMSIHLRKLSTDELLTQAFSYFDKDKNGYIEYDELREALMDDNEKVIQEIISDVDSDK 480
           FATMSIHLRK+STDELL QAFS+FDKD+NGYIEYDELREALMDD+EKVIQ+I+SDVDSDK
Sbjct: 421 FATMSIHLRKMSTDELLAQAFSFFDKDQNGYIEYDELREALMDDSEKVIQDILSDVDSDK 480

Query: 481 DGRISYNEFKAMLTTGLDWKMASRQYSRAMLHALSRKLFKD 521
           DGRISYNEFKAMLTTG+DWKM+SRQYSRAMLHALS KLFKD
Sbjct: 481 DGRISYNEFKAMLTTGMDWKMSSRQYSRAMLHALSLKLFKD 521

BLAST of MS001635 vs. NCBI nr
Match: XP_023540298.1 (calcium-dependent protein kinase 24 [Cucurbita pepo subsp. pepo] >KAG7029111.1 Calcium-dependent protein kinase 24 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1005.0 bits (2597), Expect = 2.4e-289
Identity = 493/520 (94.81%), Postives = 511/520 (98.27%), Query Frame = 0

Query: 1   MGSCVSVQARAASFAKRSRDVRPLPDHALIESVRKSSVRLAASILSDESTGDNIFEKYRF 60
           MGSCVSVQ+RA SFAKRSRDVRPLPDHA+IESVRKSSVRLAASILSDESTGDNIFEKYRF
Sbjct: 1   MGSCVSVQSRAGSFAKRSRDVRPLPDHAVIESVRKSSVRLAASILSDESTGDNIFEKYRF 60

Query: 61  GKELGRGEFGITHQCFDIETGDTFACKTISKSKLRSEINVEDVRREVAIMRNLPKHPNIV 120
           GKELGRGEFGITHQCF IETG+TFACKTISKSKLRSEINVEDVRREVAIMR+LPKHPNIV
Sbjct: 61  GKELGRGEFGITHQCFQIETGETFACKTISKSKLRSEINVEDVRREVAIMRSLPKHPNIV 120

Query: 121 AFKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICKVCHENGVIH 180
            FKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICKVCHENGVIH
Sbjct: 121 TFKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICKVCHENGVIH 180

Query: 181 RDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVLRRSYGPEID 240
           RDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVLRRSYGPEID
Sbjct: 181 RDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVLRRSYGPEID 240

Query: 241 VWSAGVILYILLCGVPPFWAESEEGIAHAIVRGDIDFERDPWPKVSKEAKELVMGMLDPN 300
           VWSAGVILYILLCGVPPFWAESEEGIAHAIVRG+IDFERDPWPKVS+EAKELVMGMLDPN
Sbjct: 241 VWSAGVILYILLCGVPPFWAESEEGIAHAIVRGNIDFERDPWPKVSQEAKELVMGMLDPN 300

Query: 301 PYSRMTVEEVLEHPWIQNKNQARNVSLGENVGLRIKQFTVMNKFKKKVLRVVADHLSDEQ 360
           PYSRMTVEEVLEHPWIQNKNQARN+ LGENVG+ IKQFT+MNKFKKKVLRVVADHLSDEQ
Sbjct: 301 PYSRMTVEEVLEHPWIQNKNQARNIPLGENVGIGIKQFTLMNKFKKKVLRVVADHLSDEQ 360

Query: 361 MDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHSLPDPDVRMLMDAADMDGNGTLSCEEF 420
           MDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHSL DPDV+MLM+AADMDGNGTLSCEEF
Sbjct: 361 MDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHSLSDPDVQMLMEAADMDGNGTLSCEEF 420

Query: 421 ATMSIHLRKLSTDELLTQAFSYFDKDKNGYIEYDELREALMDDNEKVIQEIISDVDSDKD 480
           ATMSIHLRK+STDELLTQAF +FDKD+NGYIEYDELREALMDDNEKVIQ+IISD D DKD
Sbjct: 421 ATMSIHLRKMSTDELLTQAFRFFDKDQNGYIEYDELREALMDDNEKVIQDIISDADIDKD 480

Query: 481 GRISYNEFKAMLTTGLDWKMASRQYSRAMLHALSRKLFKD 521
           GRISYNEFKAMLTTG+DWKMASRQYSRAMLHALS+KLFK+
Sbjct: 481 GRISYNEFKAMLTTGMDWKMASRQYSRAMLHALSKKLFKE 520

BLAST of MS001635 vs. NCBI nr
Match: KAG6597668.1 (Calcium-dependent protein kinase 24, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1005.0 bits (2597), Expect = 2.4e-289
Identity = 493/520 (94.81%), Postives = 511/520 (98.27%), Query Frame = 0

Query: 1   MGSCVSVQARAASFAKRSRDVRPLPDHALIESVRKSSVRLAASILSDESTGDNIFEKYRF 60
           MGSCVSVQ+RA SFAKRSRDVRPLPDHA+IESVRKSSVRLAASILSDESTGDNIFEKYRF
Sbjct: 1   MGSCVSVQSRAGSFAKRSRDVRPLPDHAVIESVRKSSVRLAASILSDESTGDNIFEKYRF 60

Query: 61  GKELGRGEFGITHQCFDIETGDTFACKTISKSKLRSEINVEDVRREVAIMRNLPKHPNIV 120
           GKELGRGEFGITHQCF IETG+TFACKTISKSKLRSEINVEDVRREVAIMR+LPKHPNIV
Sbjct: 61  GKELGRGEFGITHQCFQIETGETFACKTISKSKLRSEINVEDVRREVAIMRSLPKHPNIV 120

Query: 121 AFKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICKVCHENGVIH 180
            FKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICKVCHENGVIH
Sbjct: 121 TFKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICKVCHENGVIH 180

Query: 181 RDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVLRRSYGPEID 240
           RDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVLRRSYGPEID
Sbjct: 181 RDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVLRRSYGPEID 240

Query: 241 VWSAGVILYILLCGVPPFWAESEEGIAHAIVRGDIDFERDPWPKVSKEAKELVMGMLDPN 300
           VWSAGVILYILLCGVPPFWAESEEGIAHAIVRG+IDFERDPWPKVS+EAKELVMGMLDPN
Sbjct: 241 VWSAGVILYILLCGVPPFWAESEEGIAHAIVRGNIDFERDPWPKVSQEAKELVMGMLDPN 300

Query: 301 PYSRMTVEEVLEHPWIQNKNQARNVSLGENVGLRIKQFTVMNKFKKKVLRVVADHLSDEQ 360
           PYSRMTVEEVLEHPWIQNKNQARN+ LGENVG+ IKQFT+MNKFKKKVLRVVADHLSDEQ
Sbjct: 301 PYSRMTVEEVLEHPWIQNKNQARNIPLGENVGIGIKQFTLMNKFKKKVLRVVADHLSDEQ 360

Query: 361 MDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHSLPDPDVRMLMDAADMDGNGTLSCEEF 420
           MDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHSL DPDV+MLM+AADMDGNGTLSCEEF
Sbjct: 361 MDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHSLSDPDVQMLMEAADMDGNGTLSCEEF 420

Query: 421 ATMSIHLRKLSTDELLTQAFSYFDKDKNGYIEYDELREALMDDNEKVIQEIISDVDSDKD 480
           ATMSIHLRK+STDELLTQAF +FDKD+NGYIEYDELREALMDDNEKVIQ+IISD D DKD
Sbjct: 421 ATMSIHLRKMSTDELLTQAFRFFDKDQNGYIEYDELREALMDDNEKVIQDIISDADIDKD 480

Query: 481 GRISYNEFKAMLTTGLDWKMASRQYSRAMLHALSRKLFKD 521
           GRISYNEFKAMLTTG+DWKMASRQYSRAMLHALS+KLFK+
Sbjct: 481 GRISYNEFKAMLTTGMDWKMASRQYSRAMLHALSKKLFKE 520

BLAST of MS001635 vs. NCBI nr
Match: XP_022932619.1 (calcium-dependent protein kinase 24 [Cucurbita moschata])

HSP 1 Score: 1003.8 bits (2594), Expect = 5.4e-289
Identity = 492/520 (94.62%), Postives = 511/520 (98.27%), Query Frame = 0

Query: 1   MGSCVSVQARAASFAKRSRDVRPLPDHALIESVRKSSVRLAASILSDESTGDNIFEKYRF 60
           MGSCVSVQ+RA SFAKRSRDVRPLPDHA+IESVRKSSVRLAASILSDESTGDNIFEKYRF
Sbjct: 1   MGSCVSVQSRAGSFAKRSRDVRPLPDHAVIESVRKSSVRLAASILSDESTGDNIFEKYRF 60

Query: 61  GKELGRGEFGITHQCFDIETGDTFACKTISKSKLRSEINVEDVRREVAIMRNLPKHPNIV 120
           GKELGRGEFGITHQCF IETG+TFACKTISKSKLRSEINVEDVRREVAIMR+LPKHPNIV
Sbjct: 61  GKELGRGEFGITHQCFQIETGETFACKTISKSKLRSEINVEDVRREVAIMRSLPKHPNIV 120

Query: 121 AFKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICKVCHENGVIH 180
            FKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICKVCHENGVIH
Sbjct: 121 TFKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICKVCHENGVIH 180

Query: 181 RDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVLRRSYGPEID 240
           RDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVLRRSYGPEID
Sbjct: 181 RDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVLRRSYGPEID 240

Query: 241 VWSAGVILYILLCGVPPFWAESEEGIAHAIVRGDIDFERDPWPKVSKEAKELVMGMLDPN 300
           VWSAGVILYILLCGVPPFWAESEEGIAHAIVRG+IDFERDPWPKVS+EAKELVMGMLDPN
Sbjct: 241 VWSAGVILYILLCGVPPFWAESEEGIAHAIVRGNIDFERDPWPKVSQEAKELVMGMLDPN 300

Query: 301 PYSRMTVEEVLEHPWIQNKNQARNVSLGENVGLRIKQFTVMNKFKKKVLRVVADHLSDEQ 360
           PYSRMTVEEVLEHPWIQNKNQARN+ LGENVG+ IKQFT+MNKFKKKVLRVVADHLSDEQ
Sbjct: 301 PYSRMTVEEVLEHPWIQNKNQARNIPLGENVGIGIKQFTLMNKFKKKVLRVVADHLSDEQ 360

Query: 361 MDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHSLPDPDVRMLMDAADMDGNGTLSCEEF 420
           MDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHSL DPDV+MLM+AADMDGNGTLSCEEF
Sbjct: 361 MDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHSLSDPDVQMLMEAADMDGNGTLSCEEF 420

Query: 421 ATMSIHLRKLSTDELLTQAFSYFDKDKNGYIEYDELREALMDDNEKVIQEIISDVDSDKD 480
           ATMSIHLRK+STDELLTQAF +FDKD+NGYIEYDEL+EALMDDNEKVIQ+IISD D DKD
Sbjct: 421 ATMSIHLRKMSTDELLTQAFRFFDKDQNGYIEYDELKEALMDDNEKVIQDIISDADIDKD 480

Query: 481 GRISYNEFKAMLTTGLDWKMASRQYSRAMLHALSRKLFKD 521
           GRISYNEFKAMLTTG+DWKMASRQYSRAMLHALS+KLFK+
Sbjct: 481 GRISYNEFKAMLTTGMDWKMASRQYSRAMLHALSKKLFKE 520

BLAST of MS001635 vs. ExPASy Swiss-Prot
Match: Q9SIQ7 (Calcium-dependent protein kinase 24 OS=Arabidopsis thaliana OX=3702 GN=CPK24 PE=2 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 6.5e-205
Identity = 355/535 (66.36%), Postives = 429/535 (80.19%), Query Frame = 0

Query: 1   MGSCVSVQARAASFAKR--------SRDVRPLPDHALIESVRKSSVRLAASILSDESTGD 60
           MGSCVS   + + F KR        +     +P      ++ +  +    S +  E  GD
Sbjct: 1   MGSCVSSPLKGSPFGKRPVRRRHSSNSRTSSVPRFDSSTNLSRRLIFQPPSRVLPEPIGD 60

Query: 61  NIFEKYRFGKELGRGEFGITHQCFDIETGDTFACKTISKSKLRSEINVEDVRREVAIMRN 120
            I  KY  GKELGRGEFG+TH+C +I T + FACK ISK KLR+EI+VEDVRREV IMR 
Sbjct: 61  GIHLKYDLGKELGRGEFGVTHECIEISTRERFACKRISKEKLRTEIDVEDVRREVEIMRC 120

Query: 121 LPKHPNIVAFKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICKV 180
           LPKHPNIV+FKEAFED +AVY VME+CEGGELFDRIVS+GHYTERAAA V KTI+E+ KV
Sbjct: 121 LPKHPNIVSFKEAFEDKDAVYLVMEICEGGELFDRIVSRGHYTERAAASVAKTILEVVKV 180

Query: 181 CHENGVIHRDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVLR 240
           CHE+GVIHRDLKPENFLF++ +E ++LKAIDFGLSIFF+P QRF+EIVGSPYYMAPEVLR
Sbjct: 181 CHEHGVIHRDLKPENFLFSNGTETAQLKAIDFGLSIFFKPAQRFNEIVGSPYYMAPEVLR 240

Query: 241 RSYGPEIDVWSAGVILYILLCGVPPFWAESEEGIAHAIVRGDIDFERDPWPKVSKEAKEL 300
           R+YGPEIDVWSAGVILYILLCGVPPFWAE+EEGIAHAIVRG+IDFERDPWPKVS EAKEL
Sbjct: 241 RNYGPEIDVWSAGVILYILLCGVPPFWAETEEGIAHAIVRGNIDFERDPWPKVSHEAKEL 300

Query: 301 VMGMLDPNPYSRMTVEEVLEHPWIQNKNQARNVSLGENVGLRIKQFTVMNKFKKKVLRVV 360
           V  MLD NPYSR+TV+EVLEHPWI+N  +A NV+LG+NV  +I+QF +MN+FKKKVLR+V
Sbjct: 301 VKNMLDANPYSRLTVQEVLEHPWIRNAERAPNVNLGDNVRTKIQQFLLMNRFKKKVLRIV 360

Query: 361 ADHLSDEQMDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHSLPDPDVRMLMDAADMDGN 420
           AD+L +E++  I +MF  MDTDKNG LTFEEL++GL  IG  +PD DV+MLMDAAD DGN
Sbjct: 361 ADNLPNEEIAAIVQMFQTMDTDKNGHLTFEELRDGLKKIGQVVPDGDVKMLMDAADTDGN 420

Query: 421 GTLSCEEFATMSIHLRKLSTDELLTQAFSYFDKDKNGYIEYDELREALMDD-------NE 480
           G LSC+EF T+SIHL+++  DE L +AF YFDK+ NG+IE DEL+ AL DD       N+
Sbjct: 421 GMLSCDEFVTLSIHLKRMGCDEHLQEAFKYFDKNGNGFIELDELKVALCDDKLGHANGND 480

Query: 481 KVIQEIISDVDSDKDGRISYNEFKAMLTTGLDWKMASRQYSRAMLHALSRKLFKD 521
           + I++I  DVD +KDGRIS++EFKAM+ +G DWKMASRQYSRA+L+ALS K+FK+
Sbjct: 481 QWIKDIFFDVDLNKDGRISFDEFKAMMKSGTDWKMASRQYSRALLNALSIKMFKE 535

BLAST of MS001635 vs. ExPASy Swiss-Prot
Match: Q84SL0 (Calcium-dependent protein kinase 20 OS=Oryza sativa subsp. japonica OX=39947 GN=CPK20 PE=2 SV=2)

HSP 1 Score: 644.4 bits (1661), Expect = 1.1e-183
Identity = 307/500 (61.40%), Postives = 399/500 (79.80%), Query Frame = 0

Query: 25  PDHALIESVRKSSVRLAASILSDESTGDNIFEKYRFGKELGRGEFGITHQCFDIETGDTF 84
           P+   IE  R S+      ++  E TG +I  +Y  G ELGRGEFG+T+ C + ETGD +
Sbjct: 42  PNPFSIEYNRSSAPSGHRLVVLREPTGRDIAARYELGGELGRGEFGVTYLCTERETGDAY 101

Query: 85  ACKTISKSKLRSEINVEDVRREVAIMRNLPKHPNIVAFKEAFEDNEAVYFVMELCEGGEL 144
           ACK+ISK KLR+ +++EDVRREV IMR+LPKHPNIV  ++ +ED+ AV+ VMELCEGGEL
Sbjct: 102 ACKSISKKKLRTAVDIEDVRREVDIMRHLPKHPNIVTLRDTYEDDNAVHLVMELCEGGEL 161

Query: 145 FDRIVSKGHYTERAAADVTKTIIEICKVCHENGVIHRDLKPENFLFADESENSRLKAIDF 204
           FDRIV++GHYTERAAA VT+TI+E+ ++CH++GV+HRDLKPENFLFA++ E + LKAIDF
Sbjct: 162 FDRIVARGHYTERAAALVTRTIVEVVQMCHKHGVMHRDLKPENFLFANKKETAALKAIDF 221

Query: 205 GLSIFFEPDQRFSEIVGSPYYMAPEVLRRSYGPEIDVWSAGVILYILLCGVPPFWAESEE 264
           GLS+FF P +RF+EIVGSPYYMAPEVL+R+YGPE+DVWSAGVILYILLCGVPPFWAE+E+
Sbjct: 222 GLSVFFTPGERFTEIVGSPYYMAPEVLKRNYGPEVDVWSAGVILYILLCGVPPFWAETEQ 281

Query: 265 GIAHAIVRGDIDFERDPWPKVSKEAKELVMGMLDPNPYSRMTVEEVLEHPWIQNKNQARN 324
           G+A AI+R  IDF+RDPWP+VS  AK+LV GML+P+P  R+  ++VL+HPW+QN  +A N
Sbjct: 282 GVAQAIIRSVIDFKRDPWPRVSDNAKDLVKGMLNPDPRRRLNAQQVLDHPWLQNIKKAPN 341

Query: 325 VSLGENVGLRIKQFTVMNKFKKKVLRVVADHLSDEQMDGIRRMFHMMDTDKNGDLTFEEL 384
           V+LGE V  R++QF+VMNKFKK  LRV+A+HLS E++ GI+ MF  MD +K+  + F+EL
Sbjct: 342 VNLGETVKARLQQFSVMNKFKKHALRVIAEHLSVEEVAGIKDMFEKMDLNKDNMINFDEL 401

Query: 385 KNGLHMIGHSLPDPDVRMLMDAADMDGNGTLSCEEFATMSIHLRKLSTDELLTQAFSYFD 444
           K GLH +GH + D DV++LMDAAD+DGNG+L   EF  +S+HLRK+  DE L +AF+YFD
Sbjct: 402 KLGLHKLGHQMADADVQILMDAADVDGNGSLDYGEFVALSVHLRKIGNDEHLHKAFAYFD 461

Query: 445 KDKNGYIEYDELREALMDD----NEKVIQEIISDVDSDKDGRISYNEFKAMLTTGLDWKM 504
           ++++GYIE DELRE+L DD    +E+VI  II DVD+DKDG+ISY+EF AM+  G DW+ 
Sbjct: 462 RNQSGYIEIDELRESLADDLGANHEEVINAIIRDVDTDKDGKISYDEFAAMMKAGTDWRK 521

Query: 505 ASRQYSRAMLHALSRKLFKD 521
           ASRQYSR    +LS KL KD
Sbjct: 522 ASRQYSRERFTSLSLKLQKD 541

BLAST of MS001635 vs. ExPASy Swiss-Prot
Match: Q2QVG8 (Calcium-dependent protein kinase 29 OS=Oryza sativa subsp. japonica OX=39947 GN=CPK29 PE=2 SV=1)

HSP 1 Score: 642.9 bits (1657), Expect = 3.1e-183
Identity = 303/470 (64.47%), Postives = 386/470 (82.13%), Query Frame = 0

Query: 52  DNIFEKYRFGKELGRGEFGITHQCFDIETGDTFACKTISKSKLRSEINVEDVRREVAIMR 111
           + +  +Y  G+ELGRGEFG+T +C D  TG+  ACK+ISK KLRS ++VEDVRREVAIMR
Sbjct: 78  EEMLRRYEIGEELGRGEFGVTRRCRDAVTGERLACKSISKRKLRSSVDVEDVRREVAIMR 137

Query: 112 NLPKHPNIVAFKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICK 171
           +LP H N+V  +EAFED +AV+ VME+CEGGELFDRIV++GHYTERAAA V +TI+++ +
Sbjct: 138 SLPAHANVVRLREAFEDADAVHLVMEVCEGGELFDRIVARGHYTERAAAAVMRTIMDVVQ 197

Query: 172 VCHENGVIHRDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVL 231
            CH+NGV+HRDLKPENFL+A+ SENS LK IDFGLS+ F+P  RF+EIVGSPYYMAPEVL
Sbjct: 198 HCHKNGVMHRDLKPENFLYANASENSPLKVIDFGLSVCFKPGARFNEIVGSPYYMAPEVL 257

Query: 232 RRSYGPEIDVWSAGVILYILLCGVPPFWAESEEGIAHAIVRGDIDFERDPWPKVSKEAKE 291
           +R+YG EID+WSAGVILYILLCGVPPFWAE++EGIA AI+R  IDF+R+PWPKVS  AK+
Sbjct: 258 KRNYGQEIDIWSAGVILYILLCGVPPFWAETDEGIAQAIIRSHIDFQREPWPKVSDNAKD 317

Query: 292 LVMGMLDPNPYSRMTVEEVLEHPWIQNKNQARNVSLGENVGLRIKQFTVMNKFKKKVLRV 351
           LV  MLDPNPY+R+T ++VLEHPWIQN + A N+ LGE V  R+KQFTVMNKFKKK L V
Sbjct: 318 LVRRMLDPNPYTRLTAQQVLEHPWIQNASAAPNIPLGEAVRSRLKQFTVMNKFKKKALLV 377

Query: 352 VADHLSDEQMDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHSLPDPDVRMLMDAADMDG 411
           VA++L  E++D IR +F+M+DT K G LT EEL+ GL +IGH++ D DV MLM+AAD+DG
Sbjct: 378 VAEYLPTEELDAIRELFNMLDTKKKGHLTLEELRKGLQVIGHNIHDTDVDMLMEAADIDG 437

Query: 412 NGTLSCEEFATMSIHLRKLSTDELLTQAFSYFDKDKNGYIEYDELREALMD-DNEKVIQE 471
           NG L C+EF T+SIHL+K+ +DE L + FS+FDK+ +GYIE +EL+EAL    ++K I +
Sbjct: 438 NGILDCKEFVTVSIHLKKIRSDEHLPKVFSFFDKNGSGYIEIEELKEALSPRGDQKSIDD 497

Query: 472 IISDVDSDKDGRISYNEFKAMLTTGLDWKMASRQYSRAMLHALSRKLFKD 521
           I  DVD DKDG+ISY EF+ M++ G+DW+ ASRQYSRA+ + LSRK+FK+
Sbjct: 498 IFLDVDIDKDGKISYEEFELMMSAGMDWRNASRQYSRAVYNTLSRKIFKE 547

BLAST of MS001635 vs. ExPASy Swiss-Prot
Match: Q6NLQ6 (Calcium-dependent protein kinase 32 OS=Arabidopsis thaliana OX=3702 GN=CPK32 PE=1 SV=1)

HSP 1 Score: 641.3 bits (1653), Expect = 9.2e-183
Identity = 297/480 (61.88%), Postives = 385/480 (80.21%), Query Frame = 0

Query: 44  ILSDESTGDNIFEKYRFGKELGRGEFGITHQCFDIETGDTFACKTISKSKLRSEINVEDV 103
           I+ ++ TG  I  KY  G+ELGRGEFG+T+ C D ET D FACK+I K KLR+ +++EDV
Sbjct: 49  IVLNDPTGREIESKYTLGRELGRGEFGVTYLCTDKETDDVFACKSILKKKLRTAVDIEDV 108

Query: 104 RREVAIMRNLPKHPNIVAFKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVT 163
           RREV IMR++P+HPN+V  KE +ED  AV+ VMELCEGGELFDRIV++GHYTERAAA VT
Sbjct: 109 RREVEIMRHMPEHPNVVTLKETYEDEHAVHLVMELCEGGELFDRIVARGHYTERAAAAVT 168

Query: 164 KTIIEICKVCHENGVIHRDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSP 223
           KTI+E+ +VCH++GV+HRDLKPENFLF ++ E + LKAIDFGLS+FF+P +RF+EIVGSP
Sbjct: 169 KTIMEVVQVCHKHGVMHRDLKPENFLFGNKKETAPLKAIDFGLSVFFKPGERFNEIVGSP 228

Query: 224 YYMAPEVLRRSYGPEIDVWSAGVILYILLCGVPPFWAESEEGIAHAIVRGDIDFERDPWP 283
           YYMAPEVL+R+YGPE+D+WSAGVILYILLCGVPPFWAE+E+G+A AI+R  +DF RDPWP
Sbjct: 229 YYMAPEVLKRNYGPEVDIWSAGVILYILLCGVPPFWAETEQGVAQAIIRSVLDFRRDPWP 288

Query: 284 KVSKEAKELVMGMLDPNPYSRMTVEEVLEHPWIQNKNQARNVSLGENVGLRIKQFTVMNK 343
           KVS+ AK+L+  MLDP+   R+T ++VL+HPW+QN   A NVSLGE V  R+KQFTVMNK
Sbjct: 289 KVSENAKDLIRKMLDPDQKRRLTAQQVLDHPWLQNAKTAPNVSLGETVRARLKQFTVMNK 348

Query: 344 FKKKVLRVVADHLSDEQMDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHSLPDPDVRML 403
            KK+ LRV+A+HLSDE+  GIR  F +MDT + G +  +ELK GL  +GH++P  D+++L
Sbjct: 349 LKKRALRVIAEHLSDEEASGIREGFQIMDTSQRGKINIDELKIGLQKLGHAIPQDDLQIL 408

Query: 404 MDAADMDGNGTLSCEEFATMSIHLRKLSTDELLTQAFSYFDKDKNGYIEYDELREALMDD 463
           MDA D+D +G L C+EF  +S+HLRK+  DE L +AF++FD++ NGYIE +ELREAL D+
Sbjct: 409 MDAGDIDRDGYLDCDEFIAISVHLRKMGNDEHLKKAFAFFDQNNNGYIEIEELREALSDE 468

Query: 464 ---NEKVIQEIISDVDSDKDGRISYNEFKAMLTTGLDWKMASRQYSRAMLHALSRKLFKD 521
              +E+V+  II DVD+DKDGRISY EF  M+ TG DW+ ASRQYSR   +++S KL +D
Sbjct: 469 LGTSEEVVDAIIRDVDTDKDGRISYEEFVTMMKTGTDWRKASRQYSRERFNSISLKLMQD 528

BLAST of MS001635 vs. ExPASy Swiss-Prot
Match: Q42438 (Calcium-dependent protein kinase 8 OS=Arabidopsis thaliana OX=3702 GN=CPK8 PE=1 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 1.5e-180
Identity = 304/525 (57.90%), Postives = 408/525 (77.71%), Query Frame = 0

Query: 1   MGSCVSVQARAASFAKRSRDVRPLPDHALIESVRKSSVRLAASILSDESTGDNIFEKYRF 60
           MG+C +         K    ++  P ++   +   S      S+L D  TG +I   Y  
Sbjct: 1   MGNCCASPGSETGSKKGKPKIKSNPFYSEAYTTNGSGTGFKLSVLKD-PTGHDISLMYDL 60

Query: 61  GKELGRGEFGITHQCFDIETGDTFACKTISKSKLRSEINVEDVRREVAIMRNLPKHPNIV 120
           G+E+GRGEFGIT+ C DI+TG+ +ACK+ISK KLR+ +++EDVRREV IM+++P+HPNIV
Sbjct: 61  GREVGRGEFGITYLCTDIKTGEKYACKSISKKKLRTAVDIEDVRREVEIMKHMPRHPNIV 120

Query: 121 AFKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICKVCHENGVIH 180
           + K+AFED++AV+ VMELCEGGELFDRIV++GHYTERAAA V KTI+E+ ++CH++GV+H
Sbjct: 121 SLKDAFEDDDAVHIVMELCEGGELFDRIVARGHYTERAAAAVMKTILEVVQICHKHGVMH 180

Query: 181 RDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVLRRSYGPEID 240
           RDLKPENFLFA++ E S LKAIDFGLS+FF+P + F+EIVGSPYYMAPEVLRR+YGPE+D
Sbjct: 181 RDLKPENFLFANKKETSALKAIDFGLSVFFKPGEGFNEIVGSPYYMAPEVLRRNYGPEVD 240

Query: 241 VWSAGVILYILLCGVPPFWAESEEGIAHAIVRGDIDFERDPWPKVSKEAKELVMGMLDPN 300
           +WSAGVILYILLCGVPPFWAE+E+G+A AI+R  IDF+RDPWP+VS+ AK+LV  ML+P+
Sbjct: 241 IWSAGVILYILLCGVPPFWAETEQGVAQAIIRSVIDFKRDPWPRVSETAKDLVRKMLEPD 300

Query: 301 PYSRMTVEEVLEHPWIQNKNQARNVSLGENVGLRIKQFTVMNKFKKKVLRVVADHLSDEQ 360
           P  R++  +VLEH WIQN  +A NVSLGE V  R+KQF+VMNK KK+ LRV+A+HLS E+
Sbjct: 301 PKKRLSAAQVLEHSWIQNAKKAPNVSLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEE 360

Query: 361 MDGIRRMFHMMDTDKNGDLTFEELKNGLHMIG-HSLPDPDVRMLMDAADMDGNGTLSCEE 420
           + GI+  F MMD+ K G +  EELK GLH +G   +PD D+++LM+AAD+DG+GTL+  E
Sbjct: 361 VAGIKEAFEMMDSKKTGKINLEELKFGLHKLGQQQIPDTDLQILMEAADVDGDGTLNYGE 420

Query: 421 FATMSIHLRKLSTDELLTQAFSYFDKDKNGYIEYDELREALMDD----NEKVIQEIISDV 480
           F  +S+HL+K++ DE L +AFS+FD++++ YIE +ELREAL D+    +E+V+  I+ DV
Sbjct: 421 FVAVSVHLKKMANDEHLHKAFSFFDQNQSDYIEIEELREALNDEVDTNSEEVVAAIMQDV 480

Query: 481 DSDKDGRISYNEFKAMLTTGLDWKMASRQYSRAMLHALSRKLFKD 521
           D+DKDGRISY EF AM+  G DW+ ASRQYSR   ++LS KL ++
Sbjct: 481 DTDKDGRISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLMRE 524

BLAST of MS001635 vs. ExPASy TrEMBL
Match: A0A6J1CTJ3 (calcium-dependent protein kinase 24-like OS=Momordica charantia OX=3673 GN=LOC111014627 PE=4 SV=1)

HSP 1 Score: 1045.4 bits (2702), Expect = 7.8e-302
Identity = 519/520 (99.81%), Postives = 520/520 (100.00%), Query Frame = 0

Query: 1   MGSCVSVQARAASFAKRSRDVRPLPDHALIESVRKSSVRLAASILSDESTGDNIFEKYRF 60
           MGSCVSVQARAASFAKRSRDVRPLPDHALIESVRKSSVRLAASILSDESTGDNIFEKYRF
Sbjct: 1   MGSCVSVQARAASFAKRSRDVRPLPDHALIESVRKSSVRLAASILSDESTGDNIFEKYRF 60

Query: 61  GKELGRGEFGITHQCFDIETGDTFACKTISKSKLRSEINVEDVRREVAIMRNLPKHPNIV 120
           GKELGRGEFGITHQCFDIETGDTFACKTISKSKLRSEINVEDVRREVAIMRNLPKHPNIV
Sbjct: 61  GKELGRGEFGITHQCFDIETGDTFACKTISKSKLRSEINVEDVRREVAIMRNLPKHPNIV 120

Query: 121 AFKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICKVCHENGVIH 180
           AFKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICKVCHENGVIH
Sbjct: 121 AFKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICKVCHENGVIH 180

Query: 181 RDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVLRRSYGPEID 240
           RDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVLRRSYGPEID
Sbjct: 181 RDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVLRRSYGPEID 240

Query: 241 VWSAGVILYILLCGVPPFWAESEEGIAHAIVRGDIDFERDPWPKVSKEAKELVMGMLDPN 300
           VWSAGVILYILLCGVPPFWAESEEGIAHAIVRGDIDFERDPWPKVSKEAKELVMGMLDPN
Sbjct: 241 VWSAGVILYILLCGVPPFWAESEEGIAHAIVRGDIDFERDPWPKVSKEAKELVMGMLDPN 300

Query: 301 PYSRMTVEEVLEHPWIQNKNQARNVSLGENVGLRIKQFTVMNKFKKKVLRVVADHLSDEQ 360
           PYSRM+VEEVLEHPWIQNKNQARNVSLGENVGLRIKQFTVMNKFKKKVLRVVADHLSDEQ
Sbjct: 301 PYSRMSVEEVLEHPWIQNKNQARNVSLGENVGLRIKQFTVMNKFKKKVLRVVADHLSDEQ 360

Query: 361 MDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHSLPDPDVRMLMDAADMDGNGTLSCEEF 420
           MDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHSLPDPDVRMLMDAADMDGNGTLSCEEF
Sbjct: 361 MDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHSLPDPDVRMLMDAADMDGNGTLSCEEF 420

Query: 421 ATMSIHLRKLSTDELLTQAFSYFDKDKNGYIEYDELREALMDDNEKVIQEIISDVDSDKD 480
           ATMSIHLRKLSTDELLTQAFSYFDKDKNGYIEYDELREALMDDNEKVIQEIISDVDSDKD
Sbjct: 421 ATMSIHLRKLSTDELLTQAFSYFDKDKNGYIEYDELREALMDDNEKVIQEIISDVDSDKD 480

Query: 481 GRISYNEFKAMLTTGLDWKMASRQYSRAMLHALSRKLFKD 521
           GRISYNEFKAMLTTGLDWKMASRQYSRAMLHALSRKLFKD
Sbjct: 481 GRISYNEFKAMLTTGLDWKMASRQYSRAMLHALSRKLFKD 520

BLAST of MS001635 vs. ExPASy TrEMBL
Match: A0A6J1EXH7 (calcium-dependent protein kinase 24 OS=Cucurbita moschata OX=3662 GN=LOC111439123 PE=4 SV=1)

HSP 1 Score: 1003.8 bits (2594), Expect = 2.6e-289
Identity = 492/520 (94.62%), Postives = 511/520 (98.27%), Query Frame = 0

Query: 1   MGSCVSVQARAASFAKRSRDVRPLPDHALIESVRKSSVRLAASILSDESTGDNIFEKYRF 60
           MGSCVSVQ+RA SFAKRSRDVRPLPDHA+IESVRKSSVRLAASILSDESTGDNIFEKYRF
Sbjct: 1   MGSCVSVQSRAGSFAKRSRDVRPLPDHAVIESVRKSSVRLAASILSDESTGDNIFEKYRF 60

Query: 61  GKELGRGEFGITHQCFDIETGDTFACKTISKSKLRSEINVEDVRREVAIMRNLPKHPNIV 120
           GKELGRGEFGITHQCF IETG+TFACKTISKSKLRSEINVEDVRREVAIMR+LPKHPNIV
Sbjct: 61  GKELGRGEFGITHQCFQIETGETFACKTISKSKLRSEINVEDVRREVAIMRSLPKHPNIV 120

Query: 121 AFKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICKVCHENGVIH 180
            FKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICKVCHENGVIH
Sbjct: 121 TFKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICKVCHENGVIH 180

Query: 181 RDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVLRRSYGPEID 240
           RDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVLRRSYGPEID
Sbjct: 181 RDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVLRRSYGPEID 240

Query: 241 VWSAGVILYILLCGVPPFWAESEEGIAHAIVRGDIDFERDPWPKVSKEAKELVMGMLDPN 300
           VWSAGVILYILLCGVPPFWAESEEGIAHAIVRG+IDFERDPWPKVS+EAKELVMGMLDPN
Sbjct: 241 VWSAGVILYILLCGVPPFWAESEEGIAHAIVRGNIDFERDPWPKVSQEAKELVMGMLDPN 300

Query: 301 PYSRMTVEEVLEHPWIQNKNQARNVSLGENVGLRIKQFTVMNKFKKKVLRVVADHLSDEQ 360
           PYSRMTVEEVLEHPWIQNKNQARN+ LGENVG+ IKQFT+MNKFKKKVLRVVADHLSDEQ
Sbjct: 301 PYSRMTVEEVLEHPWIQNKNQARNIPLGENVGIGIKQFTLMNKFKKKVLRVVADHLSDEQ 360

Query: 361 MDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHSLPDPDVRMLMDAADMDGNGTLSCEEF 420
           MDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHSL DPDV+MLM+AADMDGNGTLSCEEF
Sbjct: 361 MDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHSLSDPDVQMLMEAADMDGNGTLSCEEF 420

Query: 421 ATMSIHLRKLSTDELLTQAFSYFDKDKNGYIEYDELREALMDDNEKVIQEIISDVDSDKD 480
           ATMSIHLRK+STDELLTQAF +FDKD+NGYIEYDEL+EALMDDNEKVIQ+IISD D DKD
Sbjct: 421 ATMSIHLRKMSTDELLTQAFRFFDKDQNGYIEYDELKEALMDDNEKVIQDIISDADIDKD 480

Query: 481 GRISYNEFKAMLTTGLDWKMASRQYSRAMLHALSRKLFKD 521
           GRISYNEFKAMLTTG+DWKMASRQYSRAMLHALS+KLFK+
Sbjct: 481 GRISYNEFKAMLTTGMDWKMASRQYSRAMLHALSKKLFKE 520

BLAST of MS001635 vs. ExPASy TrEMBL
Match: A0A6J1I429 (calcium-dependent protein kinase 24 OS=Cucurbita maxima OX=3661 GN=LOC111470785 PE=4 SV=1)

HSP 1 Score: 1002.3 bits (2590), Expect = 7.5e-289
Identity = 491/520 (94.42%), Postives = 510/520 (98.08%), Query Frame = 0

Query: 1   MGSCVSVQARAASFAKRSRDVRPLPDHALIESVRKSSVRLAASILSDESTGDNIFEKYRF 60
           MGSCVSVQ+RA S AKRSRDVRPLPDHA+IESVRKSSVRLAASILSD+STGDNIFEKYRF
Sbjct: 1   MGSCVSVQSRAGSLAKRSRDVRPLPDHAIIESVRKSSVRLAASILSDDSTGDNIFEKYRF 60

Query: 61  GKELGRGEFGITHQCFDIETGDTFACKTISKSKLRSEINVEDVRREVAIMRNLPKHPNIV 120
           GKELGRGEFGITHQCF IETG+TFACKTISKSKLRSEINVEDVRREVAIMR+LPKHPNIV
Sbjct: 61  GKELGRGEFGITHQCFQIETGETFACKTISKSKLRSEINVEDVRREVAIMRSLPKHPNIV 120

Query: 121 AFKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICKVCHENGVIH 180
            FKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICKVCHENGVIH
Sbjct: 121 TFKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICKVCHENGVIH 180

Query: 181 RDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVLRRSYGPEID 240
           RDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVLRRSYGPEID
Sbjct: 181 RDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVLRRSYGPEID 240

Query: 241 VWSAGVILYILLCGVPPFWAESEEGIAHAIVRGDIDFERDPWPKVSKEAKELVMGMLDPN 300
           VWSAGVILYILLCGVPPFWAESEEGIAHAIVRG+IDFERDPWPKVS+EAKELVMGMLDPN
Sbjct: 241 VWSAGVILYILLCGVPPFWAESEEGIAHAIVRGNIDFERDPWPKVSQEAKELVMGMLDPN 300

Query: 301 PYSRMTVEEVLEHPWIQNKNQARNVSLGENVGLRIKQFTVMNKFKKKVLRVVADHLSDEQ 360
           PYSRMTVEEVLEHPWIQNKNQARN+ LGENVG+ IKQFT+MNKFKKKVLRVVADHLSDEQ
Sbjct: 301 PYSRMTVEEVLEHPWIQNKNQARNIPLGENVGIGIKQFTLMNKFKKKVLRVVADHLSDEQ 360

Query: 361 MDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHSLPDPDVRMLMDAADMDGNGTLSCEEF 420
           MDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHSL DPDV+MLM+AADMDGNGTLSCEEF
Sbjct: 361 MDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHSLSDPDVQMLMEAADMDGNGTLSCEEF 420

Query: 421 ATMSIHLRKLSTDELLTQAFSYFDKDKNGYIEYDELREALMDDNEKVIQEIISDVDSDKD 480
           ATMSIHLRK+STDELLTQAF +FDKD+NGYIEYDELREALMDDNEKVIQ+IISD D DKD
Sbjct: 421 ATMSIHLRKMSTDELLTQAFRFFDKDQNGYIEYDELREALMDDNEKVIQDIISDADMDKD 480

Query: 481 GRISYNEFKAMLTTGLDWKMASRQYSRAMLHALSRKLFKD 521
           GRISYNEFKAMLTTG+DWKMASRQYSRAMLHALS+KLFK+
Sbjct: 481 GRISYNEFKAMLTTGMDWKMASRQYSRAMLHALSKKLFKE 520

BLAST of MS001635 vs. ExPASy TrEMBL
Match: A0A0A0KXB5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G011740 PE=4 SV=1)

HSP 1 Score: 995.0 bits (2571), Expect = 1.2e-286
Identity = 486/520 (93.46%), Postives = 509/520 (97.88%), Query Frame = 0

Query: 1   MGSCVSVQARAASFAKRSRDVRPLPDHALIESVRKSSVRLAASILSDESTGDNIFEKYRF 60
           MGSCVS+QAR  SF+KR++D+  LPDHA IESVRKSSVR AASILS +STGDNIFEKYRF
Sbjct: 1   MGSCVSIQARPGSFSKRTKDINTLPDHAFIESVRKSSVRHAASILSHDSTGDNIFEKYRF 60

Query: 61  GKELGRGEFGITHQCFDIETGDTFACKTISKSKLRSEINVEDVRREVAIMRNLPKHPNIV 120
           GKELGRGEFGITHQCFDIETGDTFACKTISKSKLRSEINVEDVRREVAIMR+LPKHPNIV
Sbjct: 61  GKELGRGEFGITHQCFDIETGDTFACKTISKSKLRSEINVEDVRREVAIMRSLPKHPNIV 120

Query: 121 AFKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICKVCHENGVIH 180
            FKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAA+VTKTIIEICK+CHENGVIH
Sbjct: 121 TFKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAANVTKTIIEICKLCHENGVIH 180

Query: 181 RDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVLRRSYGPEID 240
           RDLKPENFLFADESENS+LKAIDFGLSIFFEPDQRF EIVGSPYYMAPEVLRRSYGPEID
Sbjct: 181 RDLKPENFLFADESENSQLKAIDFGLSIFFEPDQRFGEIVGSPYYMAPEVLRRSYGPEID 240

Query: 241 VWSAGVILYILLCGVPPFWAESEEGIAHAIVRGDIDFERDPWPKVSKEAKELVMGMLDPN 300
           VWSAGVILYILLCGVPPFWAESEEGIAHAIVRG+IDFERDPWPKVSKEAKELV+GMLDPN
Sbjct: 241 VWSAGVILYILLCGVPPFWAESEEGIAHAIVRGNIDFERDPWPKVSKEAKELVVGMLDPN 300

Query: 301 PYSRMTVEEVLEHPWIQNKNQARNVSLGENVGLRIKQFTVMNKFKKKVLRVVADHLSDEQ 360
           PY+RMTVEEVL HPWIQNKNQARNVSLGENVG+RIKQFT+MNKFKKKVLRVVADHLSDEQ
Sbjct: 301 PYNRMTVEEVLGHPWIQNKNQARNVSLGENVGIRIKQFTLMNKFKKKVLRVVADHLSDEQ 360

Query: 361 MDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHSLPDPDVRMLMDAADMDGNGTLSCEEF 420
           M+GIRRMFHMMDTDKNGDLTFEELKNGLHMIGHSLPDPDVRMLMDAAD+DGNGTLSCEEF
Sbjct: 361 MEGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHSLPDPDVRMLMDAADLDGNGTLSCEEF 420

Query: 421 ATMSIHLRKLSTDELLTQAFSYFDKDKNGYIEYDELREALMDDNEKVIQEIISDVDSDKD 480
           ATMSIHLRK+STDELLTQAFS+FDKD+NGYIEYDELREALMDDNEKVIQ+IISDVDSDKD
Sbjct: 421 ATMSIHLRKMSTDELLTQAFSFFDKDQNGYIEYDELREALMDDNEKVIQDIISDVDSDKD 480

Query: 481 GRISYNEFKAMLTTGLDWKMASRQYSRAMLHALSRKLFKD 521
           GRISYNEFKAMLTTG+DWKM+SRQYSRAMLHALS KLFKD
Sbjct: 481 GRISYNEFKAMLTTGMDWKMSSRQYSRAMLHALSLKLFKD 520

BLAST of MS001635 vs. ExPASy TrEMBL
Match: A0A5A7TN72 (Calcium-dependent protein kinase 24 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001730 PE=4 SV=1)

HSP 1 Score: 989.9 bits (2558), Expect = 3.9e-285
Identity = 483/520 (92.88%), Postives = 507/520 (97.50%), Query Frame = 0

Query: 1   MGSCVSVQARAASFAKRSRDVRPLPDHALIESVRKSSVRLAASILSDESTGDNIFEKYRF 60
           MGSCVSVQ R  SF+KR +D+ PLPDH LIESVRKSSVR AASILS +STGDNIFEKYRF
Sbjct: 1   MGSCVSVQPRPGSFSKRPKDINPLPDHVLIESVRKSSVRHAASILSHDSTGDNIFEKYRF 60

Query: 61  GKELGRGEFGITHQCFDIETGDTFACKTISKSKLRSEINVEDVRREVAIMRNLPKHPNIV 120
           GKELGRGEFGITHQCFDIETGDTFACKTISKSKLRSEINVEDVRREVAIMR+LPKHPNIV
Sbjct: 61  GKELGRGEFGITHQCFDIETGDTFACKTISKSKLRSEINVEDVRREVAIMRSLPKHPNIV 120

Query: 121 AFKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICKVCHENGVIH 180
            FKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAA+VTKTI+EICKVCHENGVIH
Sbjct: 121 TFKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAANVTKTIVEICKVCHENGVIH 180

Query: 181 RDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVLRRSYGPEID 240
           RDLKPENFLFADESENS+LKAIDFGLSIFFEPDQRF EIVGSPYYMAPEVLRRSYGPEID
Sbjct: 181 RDLKPENFLFADESENSQLKAIDFGLSIFFEPDQRFGEIVGSPYYMAPEVLRRSYGPEID 240

Query: 241 VWSAGVILYILLCGVPPFWAESEEGIAHAIVRGDIDFERDPWPKVSKEAKELVMGMLDPN 300
           VWSAGVILYILLCGVPPFWAESEEGIAHAIVRG+IDFERDPWPKVSKEAKELV+GMLDPN
Sbjct: 241 VWSAGVILYILLCGVPPFWAESEEGIAHAIVRGNIDFERDPWPKVSKEAKELVVGMLDPN 300

Query: 301 PYSRMTVEEVLEHPWIQNKNQARNVSLGENVGLRIKQFTVMNKFKKKVLRVVADHLSDEQ 360
           PY+RMTVEEVL HPWIQNKNQARNVSLGENVG+RIKQFT+MNKFKKKVLRVVADHLSDEQ
Sbjct: 301 PYNRMTVEEVLAHPWIQNKNQARNVSLGENVGIRIKQFTLMNKFKKKVLRVVADHLSDEQ 360

Query: 361 MDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHSLPDPDVRMLMDAADMDGNGTLSCEEF 420
           M+GIRRMFHMMDTD+NGDLTFEELKNGLHMIGHSLPDPDVRMLMDAAD+DGNGTLSCEEF
Sbjct: 361 MEGIRRMFHMMDTDQNGDLTFEELKNGLHMIGHSLPDPDVRMLMDAADLDGNGTLSCEEF 420

Query: 421 ATMSIHLRKLSTDELLTQAFSYFDKDKNGYIEYDELREALMDDNEKVIQEIISDVDSDKD 480
           ATMSIHLRK+STDELLTQAF++FDK++NGYIEYDELREALMDDNEKVIQ+IISDVDSDKD
Sbjct: 421 ATMSIHLRKMSTDELLTQAFNFFDKNQNGYIEYDELREALMDDNEKVIQDIISDVDSDKD 480

Query: 481 GRISYNEFKAMLTTGLDWKMASRQYSRAMLHALSRKLFKD 521
           GRISY EFKAMLTTG+DWKM+SRQYSRAMLHALS KLFKD
Sbjct: 481 GRISYEEFKAMLTTGMDWKMSSRQYSRAMLHALSLKLFKD 520

BLAST of MS001635 vs. TAIR 10
Match: AT2G31500.1 (calcium-dependent protein kinase 24 )

HSP 1 Score: 714.9 bits (1844), Expect = 4.6e-206
Identity = 355/535 (66.36%), Postives = 429/535 (80.19%), Query Frame = 0

Query: 1   MGSCVSVQARAASFAKR--------SRDVRPLPDHALIESVRKSSVRLAASILSDESTGD 60
           MGSCVS   + + F KR        +     +P      ++ +  +    S +  E  GD
Sbjct: 1   MGSCVSSPLKGSPFGKRPVRRRHSSNSRTSSVPRFDSSTNLSRRLIFQPPSRVLPEPIGD 60

Query: 61  NIFEKYRFGKELGRGEFGITHQCFDIETGDTFACKTISKSKLRSEINVEDVRREVAIMRN 120
            I  KY  GKELGRGEFG+TH+C +I T + FACK ISK KLR+EI+VEDVRREV IMR 
Sbjct: 61  GIHLKYDLGKELGRGEFGVTHECIEISTRERFACKRISKEKLRTEIDVEDVRREVEIMRC 120

Query: 121 LPKHPNIVAFKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICKV 180
           LPKHPNIV+FKEAFED +AVY VME+CEGGELFDRIVS+GHYTERAAA V KTI+E+ KV
Sbjct: 121 LPKHPNIVSFKEAFEDKDAVYLVMEICEGGELFDRIVSRGHYTERAAASVAKTILEVVKV 180

Query: 181 CHENGVIHRDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVLR 240
           CHE+GVIHRDLKPENFLF++ +E ++LKAIDFGLSIFF+P QRF+EIVGSPYYMAPEVLR
Sbjct: 181 CHEHGVIHRDLKPENFLFSNGTETAQLKAIDFGLSIFFKPAQRFNEIVGSPYYMAPEVLR 240

Query: 241 RSYGPEIDVWSAGVILYILLCGVPPFWAESEEGIAHAIVRGDIDFERDPWPKVSKEAKEL 300
           R+YGPEIDVWSAGVILYILLCGVPPFWAE+EEGIAHAIVRG+IDFERDPWPKVS EAKEL
Sbjct: 241 RNYGPEIDVWSAGVILYILLCGVPPFWAETEEGIAHAIVRGNIDFERDPWPKVSHEAKEL 300

Query: 301 VMGMLDPNPYSRMTVEEVLEHPWIQNKNQARNVSLGENVGLRIKQFTVMNKFKKKVLRVV 360
           V  MLD NPYSR+TV+EVLEHPWI+N  +A NV+LG+NV  +I+QF +MN+FKKKVLR+V
Sbjct: 301 VKNMLDANPYSRLTVQEVLEHPWIRNAERAPNVNLGDNVRTKIQQFLLMNRFKKKVLRIV 360

Query: 361 ADHLSDEQMDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHSLPDPDVRMLMDAADMDGN 420
           AD+L +E++  I +MF  MDTDKNG LTFEEL++GL  IG  +PD DV+MLMDAAD DGN
Sbjct: 361 ADNLPNEEIAAIVQMFQTMDTDKNGHLTFEELRDGLKKIGQVVPDGDVKMLMDAADTDGN 420

Query: 421 GTLSCEEFATMSIHLRKLSTDELLTQAFSYFDKDKNGYIEYDELREALMDD-------NE 480
           G LSC+EF T+SIHL+++  DE L +AF YFDK+ NG+IE DEL+ AL DD       N+
Sbjct: 421 GMLSCDEFVTLSIHLKRMGCDEHLQEAFKYFDKNGNGFIELDELKVALCDDKLGHANGND 480

Query: 481 KVIQEIISDVDSDKDGRISYNEFKAMLTTGLDWKMASRQYSRAMLHALSRKLFKD 521
           + I++I  DVD +KDGRIS++EFKAM+ +G DWKMASRQYSRA+L+ALS K+FK+
Sbjct: 481 QWIKDIFFDVDLNKDGRISFDEFKAMMKSGTDWKMASRQYSRALLNALSIKMFKE 535

BLAST of MS001635 vs. TAIR 10
Match: AT3G57530.1 (calcium-dependent protein kinase 32 )

HSP 1 Score: 641.3 bits (1653), Expect = 6.5e-184
Identity = 297/480 (61.88%), Postives = 385/480 (80.21%), Query Frame = 0

Query: 44  ILSDESTGDNIFEKYRFGKELGRGEFGITHQCFDIETGDTFACKTISKSKLRSEINVEDV 103
           I+ ++ TG  I  KY  G+ELGRGEFG+T+ C D ET D FACK+I K KLR+ +++EDV
Sbjct: 49  IVLNDPTGREIESKYTLGRELGRGEFGVTYLCTDKETDDVFACKSILKKKLRTAVDIEDV 108

Query: 104 RREVAIMRNLPKHPNIVAFKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVT 163
           RREV IMR++P+HPN+V  KE +ED  AV+ VMELCEGGELFDRIV++GHYTERAAA VT
Sbjct: 109 RREVEIMRHMPEHPNVVTLKETYEDEHAVHLVMELCEGGELFDRIVARGHYTERAAAAVT 168

Query: 164 KTIIEICKVCHENGVIHRDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSP 223
           KTI+E+ +VCH++GV+HRDLKPENFLF ++ E + LKAIDFGLS+FF+P +RF+EIVGSP
Sbjct: 169 KTIMEVVQVCHKHGVMHRDLKPENFLFGNKKETAPLKAIDFGLSVFFKPGERFNEIVGSP 228

Query: 224 YYMAPEVLRRSYGPEIDVWSAGVILYILLCGVPPFWAESEEGIAHAIVRGDIDFERDPWP 283
           YYMAPEVL+R+YGPE+D+WSAGVILYILLCGVPPFWAE+E+G+A AI+R  +DF RDPWP
Sbjct: 229 YYMAPEVLKRNYGPEVDIWSAGVILYILLCGVPPFWAETEQGVAQAIIRSVLDFRRDPWP 288

Query: 284 KVSKEAKELVMGMLDPNPYSRMTVEEVLEHPWIQNKNQARNVSLGENVGLRIKQFTVMNK 343
           KVS+ AK+L+  MLDP+   R+T ++VL+HPW+QN   A NVSLGE V  R+KQFTVMNK
Sbjct: 289 KVSENAKDLIRKMLDPDQKRRLTAQQVLDHPWLQNAKTAPNVSLGETVRARLKQFTVMNK 348

Query: 344 FKKKVLRVVADHLSDEQMDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHSLPDPDVRML 403
            KK+ LRV+A+HLSDE+  GIR  F +MDT + G +  +ELK GL  +GH++P  D+++L
Sbjct: 349 LKKRALRVIAEHLSDEEASGIREGFQIMDTSQRGKINIDELKIGLQKLGHAIPQDDLQIL 408

Query: 404 MDAADMDGNGTLSCEEFATMSIHLRKLSTDELLTQAFSYFDKDKNGYIEYDELREALMDD 463
           MDA D+D +G L C+EF  +S+HLRK+  DE L +AF++FD++ NGYIE +ELREAL D+
Sbjct: 409 MDAGDIDRDGYLDCDEFIAISVHLRKMGNDEHLKKAFAFFDQNNNGYIEIEELREALSDE 468

Query: 464 ---NEKVIQEIISDVDSDKDGRISYNEFKAMLTTGLDWKMASRQYSRAMLHALSRKLFKD 521
              +E+V+  II DVD+DKDGRISY EF  M+ TG DW+ ASRQYSR   +++S KL +D
Sbjct: 469 LGTSEEVVDAIIRDVDTDKDGRISYEEFVTMMKTGTDWRKASRQYSRERFNSISLKLMQD 528

BLAST of MS001635 vs. TAIR 10
Match: AT5G19450.1 (calcium-dependent protein kinase 19 )

HSP 1 Score: 634.0 bits (1634), Expect = 1.0e-181
Identity = 304/525 (57.90%), Postives = 408/525 (77.71%), Query Frame = 0

Query: 1   MGSCVSVQARAASFAKRSRDVRPLPDHALIESVRKSSVRLAASILSDESTGDNIFEKYRF 60
           MG+C +         K    ++  P ++   +   S      S+L D  TG +I   Y  
Sbjct: 1   MGNCCASPGSETGSKKGKPKIKSNPFYSEAYTTNGSGTGFKLSVLKD-PTGHDISLMYDL 60

Query: 61  GKELGRGEFGITHQCFDIETGDTFACKTISKSKLRSEINVEDVRREVAIMRNLPKHPNIV 120
           G+E+GRGEFGIT+ C DI+TG+ +ACK+ISK KLR+ +++EDVRREV IM+++P+HPNIV
Sbjct: 61  GREVGRGEFGITYLCTDIKTGEKYACKSISKKKLRTAVDIEDVRREVEIMKHMPRHPNIV 120

Query: 121 AFKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICKVCHENGVIH 180
           + K+AFED++AV+ VMELCEGGELFDRIV++GHYTERAAA V KTI+E+ ++CH++GV+H
Sbjct: 121 SLKDAFEDDDAVHIVMELCEGGELFDRIVARGHYTERAAAAVMKTILEVVQICHKHGVMH 180

Query: 181 RDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVLRRSYGPEID 240
           RDLKPENFLFA++ E S LKAIDFGLS+FF+P + F+EIVGSPYYMAPEVLRR+YGPE+D
Sbjct: 181 RDLKPENFLFANKKETSALKAIDFGLSVFFKPGEGFNEIVGSPYYMAPEVLRRNYGPEVD 240

Query: 241 VWSAGVILYILLCGVPPFWAESEEGIAHAIVRGDIDFERDPWPKVSKEAKELVMGMLDPN 300
           +WSAGVILYILLCGVPPFWAE+E+G+A AI+R  IDF+RDPWP+VS+ AK+LV  ML+P+
Sbjct: 241 IWSAGVILYILLCGVPPFWAETEQGVAQAIIRSVIDFKRDPWPRVSETAKDLVRKMLEPD 300

Query: 301 PYSRMTVEEVLEHPWIQNKNQARNVSLGENVGLRIKQFTVMNKFKKKVLRVVADHLSDEQ 360
           P  R++  +VLEH WIQN  +A NVSLGE V  R+KQF+VMNK KK+ LRV+A+HLS E+
Sbjct: 301 PKKRLSAAQVLEHSWIQNAKKAPNVSLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEE 360

Query: 361 MDGIRRMFHMMDTDKNGDLTFEELKNGLHMIG-HSLPDPDVRMLMDAADMDGNGTLSCEE 420
           + GI+  F MMD+ K G +  EELK GLH +G   +PD D+++LM+AAD+DG+GTL+  E
Sbjct: 361 VAGIKEAFEMMDSKKTGKINLEELKFGLHKLGQQQIPDTDLQILMEAADVDGDGTLNYGE 420

Query: 421 FATMSIHLRKLSTDELLTQAFSYFDKDKNGYIEYDELREALMDD----NEKVIQEIISDV 480
           F  +S+HL+K++ DE L +AFS+FD++++ YIE +ELREAL D+    +E+V+  I+ DV
Sbjct: 421 FVAVSVHLKKMANDEHLHKAFSFFDQNQSDYIEIEELREALNDEVDTNSEEVVAAIMQDV 480

Query: 481 DSDKDGRISYNEFKAMLTTGLDWKMASRQYSRAMLHALSRKLFKD 521
           D+DKDGRISY EF AM+  G DW+ ASRQYSR   ++LS KL ++
Sbjct: 481 DTDKDGRISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLMRE 524

BLAST of MS001635 vs. TAIR 10
Match: AT5G19450.2 (calcium-dependent protein kinase 19 )

HSP 1 Score: 634.0 bits (1634), Expect = 1.0e-181
Identity = 304/525 (57.90%), Postives = 408/525 (77.71%), Query Frame = 0

Query: 1   MGSCVSVQARAASFAKRSRDVRPLPDHALIESVRKSSVRLAASILSDESTGDNIFEKYRF 60
           MG+C +         K    ++  P ++   +   S      S+L D  TG +I   Y  
Sbjct: 1   MGNCCASPGSETGSKKGKPKIKSNPFYSEAYTTNGSGTGFKLSVLKD-PTGHDISLMYDL 60

Query: 61  GKELGRGEFGITHQCFDIETGDTFACKTISKSKLRSEINVEDVRREVAIMRNLPKHPNIV 120
           G+E+GRGEFGIT+ C DI+TG+ +ACK+ISK KLR+ +++EDVRREV IM+++P+HPNIV
Sbjct: 61  GREVGRGEFGITYLCTDIKTGEKYACKSISKKKLRTAVDIEDVRREVEIMKHMPRHPNIV 120

Query: 121 AFKEAFEDNEAVYFVMELCEGGELFDRIVSKGHYTERAAADVTKTIIEICKVCHENGVIH 180
           + K+AFED++AV+ VMELCEGGELFDRIV++GHYTERAAA V KTI+E+ ++CH++GV+H
Sbjct: 121 SLKDAFEDDDAVHIVMELCEGGELFDRIVARGHYTERAAAAVMKTILEVVQICHKHGVMH 180

Query: 181 RDLKPENFLFADESENSRLKAIDFGLSIFFEPDQRFSEIVGSPYYMAPEVLRRSYGPEID 240
           RDLKPENFLFA++ E S LKAIDFGLS+FF+P + F+EIVGSPYYMAPEVLRR+YGPE+D
Sbjct: 181 RDLKPENFLFANKKETSALKAIDFGLSVFFKPGEGFNEIVGSPYYMAPEVLRRNYGPEVD 240

Query: 241 VWSAGVILYILLCGVPPFWAESEEGIAHAIVRGDIDFERDPWPKVSKEAKELVMGMLDPN 300
           +WSAGVILYILLCGVPPFWAE+E+G+A AI+R  IDF+RDPWP+VS+ AK+LV  ML+P+
Sbjct: 241 IWSAGVILYILLCGVPPFWAETEQGVAQAIIRSVIDFKRDPWPRVSETAKDLVRKMLEPD 300

Query: 301 PYSRMTVEEVLEHPWIQNKNQARNVSLGENVGLRIKQFTVMNKFKKKVLRVVADHLSDEQ 360
           P  R++  +VLEH WIQN  +A NVSLGE V  R+KQF+VMNK KK+ LRV+A+HLS E+
Sbjct: 301 PKKRLSAAQVLEHSWIQNAKKAPNVSLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEE 360

Query: 361 MDGIRRMFHMMDTDKNGDLTFEELKNGLHMIG-HSLPDPDVRMLMDAADMDGNGTLSCEE 420
           + GI+  F MMD+ K G +  EELK GLH +G   +PD D+++LM+AAD+DG+GTL+  E
Sbjct: 361 VAGIKEAFEMMDSKKTGKINLEELKFGLHKLGQQQIPDTDLQILMEAADVDGDGTLNYGE 420

Query: 421 FATMSIHLRKLSTDELLTQAFSYFDKDKNGYIEYDELREALMDD----NEKVIQEIISDV 480
           F  +S+HL+K++ DE L +AFS+FD++++ YIE +ELREAL D+    +E+V+  I+ DV
Sbjct: 421 FVAVSVHLKKMANDEHLHKAFSFFDQNQSDYIEIEELREALNDEVDTNSEEVVAAIMQDV 480

Query: 481 DSDKDGRISYNEFKAMLTTGLDWKMASRQYSRAMLHALSRKLFKD 521
           D+DKDGRISY EF AM+  G DW+ ASRQYSR   ++LS KL ++
Sbjct: 481 DTDKDGRISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLMRE 524

BLAST of MS001635 vs. TAIR 10
Match: AT5G12480.1 (calmodulin-domain protein kinase 7 )

HSP 1 Score: 630.9 bits (1626), Expect = 8.8e-181
Identity = 300/491 (61.10%), Postives = 391/491 (79.63%), Query Frame = 0

Query: 35  KSSVRLAASILSDESTGDNIFEKYRFGKELGRGEFGITHQCFDIETGDTFACKTISKSKL 94
           +S      S+L D  TG +I  +Y  G+E+GRGEFGIT+ C D ETG+ +ACK+ISK KL
Sbjct: 37  RSGAGFKLSVLKD-PTGHDISLQYDLGREVGRGEFGITYLCTDKETGEKYACKSISKKKL 96

Query: 95  RSEINVEDVRREVAIMRNLPKHPNIVAFKEAFEDNEAVYFVMELCEGGELFDRIVSKGHY 154
           R+ +++EDVRREV IM+++PKHPN+V+ K++FED++AV+ VMELCEGGELFDRIV++GHY
Sbjct: 97  RTAVDIEDVRREVEIMKHMPKHPNVVSLKDSFEDDDAVHIVMELCEGGELFDRIVARGHY 156

Query: 155 TERAAADVTKTIIEICKVCHENGVIHRDLKPENFLFADESENSRLKAIDFGLSIFFEPDQ 214
           TERAAA V KTI+E+ ++CH+ GV+HRDLKPENFLFA++ E S LKAIDFGLS+FF+P +
Sbjct: 157 TERAAAAVMKTIVEVVQICHKQGVMHRDLKPENFLFANKKETSALKAIDFGLSVFFKPGE 216

Query: 215 RFSEIVGSPYYMAPEVLRRSYGPEIDVWSAGVILYILLCGVPPFWAESEEGIAHAIVRGD 274
           +F+EIVGSPYYMAPEVLRR+YGPEIDVWSAGVILYILLCGVPPFWAE+E+G+A AI+R  
Sbjct: 217 QFNEIVGSPYYMAPEVLRRNYGPEIDVWSAGVILYILLCGVPPFWAETEQGVAQAIIRSV 276

Query: 275 IDFERDPWPKVSKEAKELVMGMLDPNPYSRMTVEEVLEHPWIQNKNQARNVSLGENVGLR 334
           IDF+RDPWP+VS  AK+LV  ML+P+P  R+T  +VLEH WI N  +A NVSLGE V  R
Sbjct: 277 IDFKRDPWPRVSDSAKDLVRKMLEPDPKKRLTAAQVLEHTWILNAKKAPNVSLGETVKAR 336

Query: 335 IKQFTVMNKFKKKVLRVVADHLSDEQMDGIRRMFHMMDTDKNGDLTFEELKNGLHMIGHS 394
           +KQF+VMNK KK+ LRV+A+HLS E+  GI+  F MMD +K G +  EELK GL   G  
Sbjct: 337 LKQFSVMNKLKKRALRVIAEHLSVEEAAGIKEAFEMMDVNKRGKINLEELKYGLQKAGQQ 396

Query: 395 LPDPDVRMLMDAADMDGNGTLSCEEFATMSIHLRKLSTDELLTQAFSYFDKDKNGYIEYD 454
           + D D+++LM+A D+DG+GTL+  EF  +S+HL+K++ DE L +AF++FD++++GYIE D
Sbjct: 397 IADTDLQILMEATDVDGDGTLNYSEFVAVSVHLKKMANDEHLHKAFNFFDQNQSGYIEID 456

Query: 455 ELREALMDD-----NEKVIQEIISDVDSDKDGRISYNEFKAMLTTGLDWKMASRQYSRAM 514
           ELREAL D+     +E+VI  I+ DVD+DKDGRISY EF AM+  G DW+ ASRQYSR  
Sbjct: 457 ELREALNDELDNTSSEEVIAAIMQDVDTDKDGRISYEEFVAMMKAGTDWRKASRQYSRER 516

Query: 515 LHALSRKLFKD 521
            ++LS KL +D
Sbjct: 517 FNSLSLKLMRD 526

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022145110.11.6e-30199.81calcium-dependent protein kinase 24-like [Momordica charantia][more]
XP_038877045.11.3e-29095.39calcium-dependent protein kinase 24 [Benincasa hispida][more]
XP_023540298.12.4e-28994.81calcium-dependent protein kinase 24 [Cucurbita pepo subsp. pepo] >KAG7029111.1 C... [more]
KAG6597668.12.4e-28994.81Calcium-dependent protein kinase 24, partial [Cucurbita argyrosperma subsp. soro... [more]
XP_022932619.15.4e-28994.62calcium-dependent protein kinase 24 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9SIQ76.5e-20566.36Calcium-dependent protein kinase 24 OS=Arabidopsis thaliana OX=3702 GN=CPK24 PE=... [more]
Q84SL01.1e-18361.40Calcium-dependent protein kinase 20 OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Q2QVG83.1e-18364.47Calcium-dependent protein kinase 29 OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Q6NLQ69.2e-18361.88Calcium-dependent protein kinase 32 OS=Arabidopsis thaliana OX=3702 GN=CPK32 PE=... [more]
Q424381.5e-18057.90Calcium-dependent protein kinase 8 OS=Arabidopsis thaliana OX=3702 GN=CPK8 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1CTJ37.8e-30299.81calcium-dependent protein kinase 24-like OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A6J1EXH72.6e-28994.62calcium-dependent protein kinase 24 OS=Cucurbita moschata OX=3662 GN=LOC11143912... [more]
A0A6J1I4297.5e-28994.42calcium-dependent protein kinase 24 OS=Cucurbita maxima OX=3661 GN=LOC111470785 ... [more]
A0A0A0KXB51.2e-28693.46Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G011740 PE=4 SV=1[more]
A0A5A7TN723.9e-28592.88Calcium-dependent protein kinase 24 OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
Match NameE-valueIdentityDescription
AT2G31500.14.6e-20666.36calcium-dependent protein kinase 24 [more]
AT3G57530.16.5e-18461.88calcium-dependent protein kinase 32 [more]
AT5G19450.11.0e-18157.90calcium-dependent protein kinase 19 [more]
AT5G19450.21.0e-18157.90calcium-dependent protein kinase 19 [more]
AT5G12480.18.8e-18161.10calmodulin-domain protein kinase 7 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 363..391
e-value: 6.2E-5
score: 32.4
coord: 435..463
e-value: 3.2E-4
score: 30.0
coord: 467..495
e-value: 9.2E-6
score: 35.1
coord: 399..427
e-value: 0.55
score: 19.3
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 435..492
e-value: 1.2E-11
score: 44.8
IPR002048EF-hand domainPFAMPF13833EF-hand_8coord: 376..423
e-value: 8.5E-9
score: 35.1
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 463..498
score: 14.039183
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 431..460
score: 10.273304
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 395..430
score: 8.794848
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 359..394
score: 14.708673
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 58..316
e-value: 6.0E-98
score: 341.4
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 58..316
e-value: 8.8E-74
score: 248.2
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 58..316
score: 48.654186
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 144..325
e-value: 4.9E-60
score: 204.1
NoneNo IPR availableGENE3D1.10.238.10coord: 326..494
e-value: 1.1E-49
score: 170.5
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 60..143
e-value: 7.8E-27
score: 95.5
NoneNo IPR availablePIRSRPIRSR037568-2PIRSR037568-2coord: 56..320
e-value: 3.3E-30
score: 102.1
NoneNo IPR availablePIRSRPIRSR038172-1PIRSR038172-1coord: 56..258
e-value: 3.4E-23
score: 79.7
NoneNo IPR availablePIRSRPIRSR633573-1PIRSR633573-1coord: 6..262
e-value: 3.9E-16
score: 57.0
NoneNo IPR availablePANTHERPTHR24349:SF160CALCIUM-DEPENDENT PROTEIN KINASE 24coord: 44..507
NoneNo IPR availablePANTHERPTHR24349SERINE/THREONINE-PROTEIN KINASEcoord: 44..507
NoneNo IPR availableCDDcd05117STKc_CAMKcoord: 57..315
e-value: 8.56213E-133
score: 383.75
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 476..488
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 408..420
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 372..384
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 64..87
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 178..190
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 46..324
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 352..494

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS001635.1MS001635.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0004672 protein kinase activity