MS001008 (gene) Bitter gourd (TR) v1

Overview


Name	MS001008
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	AB hydrolase-1 domain-containing protein
Location	scaffold36: 826552 .. 829434 (+)
RNA-Seq Expression	MS001008
Synteny	MS001008

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTCGCTCTAACAGTGGCTTCCAGTTTGGGCTCCGCTCCACTGCACACGAAATGGAAGAAACCGAAGCGTCTAGGGTTCACCATTAACGAATTTCCTTCATTCCTTCCCAAAGAAGTCGAGAATATCAAAGACCAGTTTGCTCGGAAGCTCGCTACAAGAATTGAGAGGCTACCCGTAAGTTCGAATTCTCTTGAAAATTCATACTATTTGGTTGTTGTCCAGTCGCGTGCTAATTAGTTGGGATAGACTGGGGAAAATGGAAACTGGACCTCGCCATGCGAGGGAGATAAAGCCATGTTTCTTTTGGCATTCTGCCATCCAATTTGATCAAAGGGTTCTTTACTTCTGAGGGATTTTCAGTGTTCCTTATCTGACTTATATTGTTCCCGCTCTGCATGCAGGTCAGCTTCTCTGACACTTGTATTATGAGCAGTTTCGTGAAGCCGTCAATACAGAGCAAAGCAAGTCCAGTGGTTCTTCTCCATGGATTTGACAGGTTCCTTCACTTACTAGTTACAATAATCCAACACAATTGTTTATTTTATTTTTTGTTTATCCTTTCATCGTTCATCACATCTTATATTCAGCTCCTGTTTAGAATGGAGATACACATATCCATTGCTCGAGGAAGCTGGTTTAGAAACCTGGGCTGTCGACATTCTTGGCTGGGGCTTTTCTGATTTAGGTCTGTCTAATGGCTGTATTCCTCTTTTCGTTGATCGTTATTCCCATCATTTCTGTCTCTAATTTTCGCAGTTGCATGTGATAGAAAGACTGCCACCATGTGACGTGACTTCTAAACGCGATCATTTGTATCAGGTGATGAAAACGTTCTATTAAGATCTGTGTTATTCTTATTAAAAATAAGATGATTTTCCTGTTATGTACTTGGGGAAATGTTCTCTTCAATTCCAGTTATGCTTCCTTCCAATATGATCATAATTGGACTTTCCAAGCACATACCCTGTTGTGCTCTTTTAATAATATGACACTTACAGCATTGCTATTAGCTTATTTCATTTTGCTGTCGTTTCTATAGACTTGTTCATAGTTATCCTGTTCTTTTCATTCTTTTTATCTTTTTTTGCATAATTTCTGATTGTTGATCCCAATTGTATTTTGCCCGGTTATGTTCAAACTAACTAAGTTTTTGCTCTGTCCTGACGATAGCTTTGGAAATCTTATATTAAAAGGCCAATGGTATTGGTTGGACCCAGCCTTGGCGCTGCTGTTGCTATTGATTTTGCAGTCAATCATCCAGAAGCTGTAAGTATCTCCAAATCAAATTTATGACTACAAAATTTAAGTTATATCCCAACTCAAAGTTATCCTTGAGTTTTCTGTTTAATGTAATGGGATGATGTCAGGTTGATAGGCTTGTGTTGATTGATGCAAGTGTATATGCAGAAGGTACAGGAAACTTAGCTACTCTACCAAGATTGATTGCCTATGCTGGGGTGAGTTTTTGCTCACTTCTTTGAACATTCCATCGAGTGTGCTAGCTAAGCATTTTCCTTCATAGTTTTAATTTATGCAATTACGAGACAGAACCACAAACTCTTGTTTGACAGGAAAACATGGAAGATATTGCGTAGCACCACCAAGAAACTTTCATATTAATTCTTCTGCAAAATCTTTATGGCGAGAGTTATCTAGGTCGATTGTTGTATGGCATTGTCATAACTGCAGTGGCTTATTTTGATCAGGTATTTTTATTGAAGAGCGTTCCCCTGCGCGTATATGTGAATCTTTTAGCCTTCACTGACATATCATTTAGTACCAACCTGGATTGGAGCAATGTAAGTTTTTTCCTGCATAAAGGAGAATAAATTATCATATCTTGTTGACTTGACGTGTATCTTGTTAGACTGAAGGCTTAATTGACATAAAGATTAAATGCTATGTGTTTATAACCAATCGACTCACCCCCTGATCCTACTATTTTTCTTCTATCCCTACTAATATTTTATTAGATCGGCCGCCTGCATTGTTTACTGCCTTGGTGGGAGGATGCGACTGTTAAATTTATGTTAAGTGGAGGATATGATGTCGGTGGCCAGATTGAAAAGGTTAGTACAATTTCTTTGATATTATATGTCTGATGAAGTCGCATCAGCCATATCAATGAAAAGTTGTATGTATGTTAGTGTGAGGTAGGGAAAGATCTCATTCCTCATCAAGAAAGGGTAGTTCAAATGACTTTTCTTTAATGTCGTTGAAGAAATAAGAATGAATGCCTTGTCTTATGGTTTATTTGCCAATTATCTTATTAACATTTTTATCTAACCAAAAAAAAAAAAAACAGTTATCAGAATTTGAGAATCTTTAGGTAATTCTGGTTTAATAGGTGAAGCAGAAGACGCTTATTATATGGGGCGAGGATGATCAAATCATCAGCAACAAACTTGCAGTGGTTAGTGTTTATTGCAATTCTAAAAAATGAGACAGGTAAAATTGTTTTAGGTTGCCAGAAAATATTGTGAGAAAGGCCGCGAAGGATATAGCAAACAAGAAAAATCTTTTATTTTTACCACCTGACCCTACTACGTTGATTACCAACAAATTGATAGGATTTTAAATCCTAGGTAGGTGGACATAAATCAATTCCTTCCCCAAGCTCCTTTATTTTTTAAGTGCCAAGTGGCTTTATCGTCTTTTGCCTTTTTGCATTGAATCATTATTGTTAGAATAACCTCACCACATGATTTTTCTAAAAATGCAATGCAGAGGTTGCACTGTGAACTGCCAAATGCAGTTATTCGTCAAATACCTGAATGCGGCCACCTTCCTCATGTCGAGAAGCCAAGTTTTGTTGCCAAATTGATTATGCAATTTGTTCACGAAGATTCCATAAAAGAGGTTCAGTATGTTAATAGTGGA

mRNA sequence

ATGCTCGCTCTAACAGTGGCTTCCAGTTTGGGCTCCGCTCCACTGCACACGAAATGGAAGAAACCGAAGCGTCTAGGGTTCACCATTAACGAATTTCCTTCATTCCTTCCCAAAGAAGTCGAGAATATCAAAGACCAGTTTGCTCGGAAGCTCGCTACAAGAATTGAGAGGCTACCCGTCAGCTTCTCTGACACTTGTATTATGAGCAGTTTCGTGAAGCCGTCAATACAGAGCAAAGCAAGTCCAGTGGTTCTTCTCCATGGATTTGACAGCTCCTGTTTAGAATGGAGATACACATATCCATTGCTCGAGGAAGCTGGTTTAGAAACCTGGGCTGTCGACATTCTTGGCTGGGGCTTTTCTGATTTAGAAAGACTGCCACCATGTGACGTGACTTCTAAACGCGATCATTTGTATCAGCTTTGGAAATCTTATATTAAAAGGCCAATGGTATTGGTTGGACCCAGCCTTGGCGCTGCTGTTGCTATTGATTTTGCAGTCAATCATCCAGAAGCTGTTGATAGGCTTGTGTTGATTGATGCAAGTGTATATGCAGAAGGTACAGGAAACTTAGCTACTCTACCAAGATTGATTGCCTATGCTGGGGTATTTTTATTGAAGAGCGTTCCCCTGCGCGTATATGTGAATCTTTTAGCCTTCACTGACATATCATTTAGTACCAACCTGGATTGGAGCAATATCGGCCGCCTGCATTGTTTACTGCCTTGGTGGGAGGATGCGACTGTTAAATTTATGTTAAGTGGAGGATATGATGTCGGTGGCCAGATTGAAAAGGTGAAGCAGAAGACGCTTATTATATGGGGCGAGGATGATCAAATCATCAGCAACAAACTTGCAGTGAGGTTGCACTGTGAACTGCCAAATGCAGTTATTCGTCAAATACCTGAATGCGGCCACCTTCCTCATGTCGAGAAGCCAAGTTTTGTTGCCAAATTGATTATGCAATTTGTTCACGAAGATTCCATAAAAGAGGTTCAGTATGTTAATAGTGGA

Coding sequence (CDS)

ATGCTCGCTCTAACAGTGGCTTCCAGTTTGGGCTCCGCTCCACTGCACACGAAATGGAAGAAACCGAAGCGTCTAGGGTTCACCATTAACGAATTTCCTTCATTCCTTCCCAAAGAAGTCGAGAATATCAAAGACCAGTTTGCTCGGAAGCTCGCTACAAGAATTGAGAGGCTACCCGTCAGCTTCTCTGACACTTGTATTATGAGCAGTTTCGTGAAGCCGTCAATACAGAGCAAAGCAAGTCCAGTGGTTCTTCTCCATGGATTTGACAGCTCCTGTTTAGAATGGAGATACACATATCCATTGCTCGAGGAAGCTGGTTTAGAAACCTGGGCTGTCGACATTCTTGGCTGGGGCTTTTCTGATTTAGAAAGACTGCCACCATGTGACGTGACTTCTAAACGCGATCATTTGTATCAGCTTTGGAAATCTTATATTAAAAGGCCAATGGTATTGGTTGGACCCAGCCTTGGCGCTGCTGTTGCTATTGATTTTGCAGTCAATCATCCAGAAGCTGTTGATAGGCTTGTGTTGATTGATGCAAGTGTATATGCAGAAGGTACAGGAAACTTAGCTACTCTACCAAGATTGATTGCCTATGCTGGGGTATTTTTATTGAAGAGCGTTCCCCTGCGCGTATATGTGAATCTTTTAGCCTTCACTGACATATCATTTAGTACCAACCTGGATTGGAGCAATATCGGCCGCCTGCATTGTTTACTGCCTTGGTGGGAGGATGCGACTGTTAAATTTATGTTAAGTGGAGGATATGATGTCGGTGGCCAGATTGAAAAGGTGAAGCAGAAGACGCTTATTATATGGGGCGAGGATGATCAAATCATCAGCAACAAACTTGCAGTGAGGTTGCACTGTGAACTGCCAAATGCAGTTATTCGTCAAATACCTGAATGCGGCCACCTTCCTCATGTCGAGAAGCCAAGTTTTGTTGCCAAATTGATTATGCAATTTGTTCACGAAGATTCCATAAAAGAGGTTCAGTATGTTAATAGTGGA

Protein sequence

MLALTVASSLGSAPLHTKWKKPKRLGFTINEFPSFLPKEVENIKDQFARKLATRIERLPVSFSDTCIMSSFVKPSIQSKASPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGFSDLERLPPCDVTSKRDHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNHPEAVDRLVLIDASVYAEGTGNLATLPRLIAYAGVFLLKSVPLRVYVNLLAFTDISFSTNLDWSNIGRLHCLLPWWEDATVKFMLSGGYDVGGQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAVIRQIPECGHLPHVEKPSFVAKLIMQFVHEDSIKEVQYVNSG

Homology

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BLAST of MS001008 vs. NCBI nr
Match: XP_022131473.1 (uncharacterized protein LOC111004671 isoform X1 [Momordica charantia])

HSP 1 Score: 677.2 bits (1746), Expect = 7.5e-191
Identity = 330/335 (98.51%), Postives = 331/335 (98.81%), Query Frame = 0


Query: 1   MLALTVASSLGSAPLHTKWKKPKRLGFTINEFPSFLPKEVENIKDQFARKLATRIERLPV 60
           MLALTVASSLGSAPLHTKWKKPKRLGFTINEFPSFLPKEVENIKDQFARKLATRIERLPV
Sbjct: 1   MLALTVASSLGSAPLHTKWKKPKRLGFTINEFPSFLPKEVENIKDQFARKLATRIERLPV 60

Query: 61  SFSDTCIMSSFVKPSIQSKASPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGF 120
           SFSDTCIMSSFVKPSIQSKASPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGF
Sbjct: 61  SFSDTCIMSSFVKPSIQSKASPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGF 120

Query: 121 SDLERLPPCDVTSKRDHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNHPEAVDRLVLID 180
           SDLERLPPCDVTSKRDHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNHPEAVDRLVLID
Sbjct: 121 SDLERLPPCDVTSKRDHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNHPEAVDRLVLID 180

Query: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSVPLRVYVNLLAFTDISFSTNLDWSNIGRLHCL 240
           ASVYAEGTGNLATLPRLIAYAGVFLLKSVPLRVYVNLLAFTDISFSTNLDWSNIGRLHCL
Sbjct: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSVPLRVYVNLLAFTDISFSTNLDWSNIGRLHCL 240

Query: 241 LPWWEDATVKFMLSGGYDVGGQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAVIRQ 300
           LPWWEDATVKFM SGGYDVGGQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAVIRQ
Sbjct: 241 LPWWEDATVKFMSSGGYDVGGQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAVIRQ 300

Query: 301 IPECGHLPHVEKPSFVAKLIMQFVHEDSIKEVQYV 336
           IPECGHLPHVEKPSFVAKLIMQFVHEDS KEV  +
Sbjct: 301 IPECGHLPHVEKPSFVAKLIMQFVHEDSRKEVDSI 335

[Close]

BLAST of MS001008 vs. NCBI nr
Match: XP_022131474.1 (uncharacterized protein LOC111004671 isoform X2 [Momordica charantia])

HSP 1 Score: 675.2 bits (1741), Expect = 2.8e-190
Identity = 329/331 (99.40%), Postives = 329/331 (99.40%), Query Frame = 0


Query: 1   MLALTVASSLGSAPLHTKWKKPKRLGFTINEFPSFLPKEVENIKDQFARKLATRIERLPV 60
           MLALTVASSLGSAPLHTKWKKPKRLGFTINEFPSFLPKEVENIKDQFARKLATRIERLPV
Sbjct: 1   MLALTVASSLGSAPLHTKWKKPKRLGFTINEFPSFLPKEVENIKDQFARKLATRIERLPV 60

Query: 61  SFSDTCIMSSFVKPSIQSKASPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGF 120
           SFSDTCIMSSFVKPSIQSKASPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGF
Sbjct: 61  SFSDTCIMSSFVKPSIQSKASPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGF 120

Query: 121 SDLERLPPCDVTSKRDHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNHPEAVDRLVLID 180
           SDLERLPPCDVTSKRDHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNHPEAVDRLVLID
Sbjct: 121 SDLERLPPCDVTSKRDHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNHPEAVDRLVLID 180

Query: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSVPLRVYVNLLAFTDISFSTNLDWSNIGRLHCL 240
           ASVYAEGTGNLATLPRLIAYAGVFLLKSVPLRVYVNLLAFTDISFSTNLDWSNIGRLHCL
Sbjct: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSVPLRVYVNLLAFTDISFSTNLDWSNIGRLHCL 240

Query: 241 LPWWEDATVKFMLSGGYDVGGQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAVIRQ 300
           LPWWEDATVKFM SGGYDVGGQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAVIRQ
Sbjct: 241 LPWWEDATVKFMSSGGYDVGGQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAVIRQ 300

Query: 301 IPECGHLPHVEKPSFVAKLIMQFVHEDSIKE 332
           IPECGHLPHVEKPSFVAKLIMQFVHEDS KE
Sbjct: 301 IPECGHLPHVEKPSFVAKLIMQFVHEDSRKE 331

[Close]

BLAST of MS001008 vs. NCBI nr
Match: XP_038884275.1 (uncharacterized hydrolase YugF isoform X2 [Benincasa hispida])

HSP 1 Score: 614.4 bits (1583), Expect = 5.9e-172
Identity = 297/337 (88.13%), Postives = 314/337 (93.18%), Query Frame = 0


Query: 1   MLALTVASSLGSAPLHTKWKKPKRLGFTINEFPSFLPKEVENIKDQFARKLATRIERLPV 60
           ML+LT+A+S GSAPLH +WKK K L  TINEFPSFLPKEV NIKDQFAR LATRIERLPV
Sbjct: 1   MLSLTMAASWGSAPLHIQWKKAKHLCVTINEFPSFLPKEVHNIKDQFARNLATRIERLPV 60

Query: 61  SFSDTCIMSSFVKPSIQSKASPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGF 120
           SFSD+CIMSS VKPSIQSK  PVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGF
Sbjct: 61  SFSDSCIMSSCVKPSIQSKERPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGF 120

Query: 121 SDLERLPPCDVTSKRDHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNHPEAVDRLVLID 180
           SDLERLPPCDVTSKR HLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVN+P+AVDRLVLID
Sbjct: 121 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPKAVDRLVLID 180

Query: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSVPLRVYVNLLAFTDISFSTNLDWSNIGRLHCL 240
           ASVYAEGTGNLATLPRLIAYAGVFLLKS+PLR+YVN+L F  ISFST+LDW+NIGRLHCL
Sbjct: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLTFAGISFSTSLDWANIGRLHCL 240

Query: 241 LPWWEDATVKFMLSGGYDVGGQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAVIRQ 300
           LPWWEDATV FMLSGGY+V  QIEKVKQK+LIIWGEDDQIIS+KL VRLHCELPNA+IRQ
Sbjct: 241 LPWWEDATVSFMLSGGYNVSSQIEKVKQKSLIIWGEDDQIISSKLGVRLHCELPNAIIRQ 300

Query: 301 IPECGHLPHVEKPSFVAKLIMQFVHEDSIKEVQYVNS 338
           IP CGHLPHVEKP+ VAKLI QFVHEDS KEVQYVNS
Sbjct: 301 IPGCGHLPHVEKPNLVAKLITQFVHEDSRKEVQYVNS 337

[Close]

BLAST of MS001008 vs. NCBI nr
Match: KAG7029571.1 (yugF, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 611.3 bits (1575), Expect = 5.0e-171
Identity = 297/337 (88.13%), Postives = 313/337 (92.88%), Query Frame = 0


Query: 1   MLALTVASSLGSAPLHTKWKKPKRLGFTINEFPSFLPKEVENIKDQFARKLATRIERLPV 60
           ML+L VA+S  SAP   +WKKPKRLG  +N+FPSFLPKEV+NIKDQFAR+LATRIERLPV
Sbjct: 27  MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV 86

Query: 61  SFSDTCIMSSFVKPSIQSKASPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGF 120
           SFSD+C MSS VKPSIQSK  PVVLLHGFDSSCLEWRYTYPLLEEAG ETWAVDILGWGF
Sbjct: 87  SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 146

Query: 121 SDLERLPPCDVTSKRDHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNHPEAVDRLVLID 180
           SDLERLPPCDVTSKR HLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVN+PEAVDRLVLID
Sbjct: 147 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID 206

Query: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSVPLRVYVNLLAFTDISFSTNLDWSNIGRLHCL 240
           ASVYAEGTGNLATLPRLIAYAGVFLLKS+PLR+YVN+LAFT IS ST+LDW NIGRLHCL
Sbjct: 207 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 266

Query: 241 LPWWEDATVKFMLSGGYDVGGQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAVIRQ 300
           LPWWEDATV FMLSGGY+V  QIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNA IRQ
Sbjct: 267 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 326

Query: 301 IPECGHLPHVEKPSFVAKLIMQFVHEDSIKEVQYVNS 338
           IPECGHLPHVEKP+FVAKLI QFVHEDS KEV YVN+
Sbjct: 327 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEVPYVNN 363

[Close]

BLAST of MS001008 vs. NCBI nr
Match: XP_022962117.1 (uncharacterized protein LOC111462669 isoform X1 [Cucurbita moschata])

HSP 1 Score: 611.3 bits (1575), Expect = 5.0e-171
Identity = 297/337 (88.13%), Postives = 313/337 (92.88%), Query Frame = 0


Query: 1   MLALTVASSLGSAPLHTKWKKPKRLGFTINEFPSFLPKEVENIKDQFARKLATRIERLPV 60
           ML+L VA+S  SAP   +WKKPKRLG  +N+FPSFLPKEV+NIKDQFAR+LATRIERLPV
Sbjct: 1   MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV 60

Query: 61  SFSDTCIMSSFVKPSIQSKASPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGF 120
           SFSD+C MSS VKPSIQSK  PVVLLHGFDSSCLEWRYTYPLLEEAG ETWAVDILGWGF
Sbjct: 61  SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 120

Query: 121 SDLERLPPCDVTSKRDHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNHPEAVDRLVLID 180
           SDLERLPPCDVTSKR HLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVN+PEAVDRLVLID
Sbjct: 121 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID 180

Query: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSVPLRVYVNLLAFTDISFSTNLDWSNIGRLHCL 240
           ASVYAEGTGNLATLPRLIAYAGVFLLKS+PLR+YVN+LAFT IS ST+LDW NIGRLHCL
Sbjct: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 240

Query: 241 LPWWEDATVKFMLSGGYDVGGQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAVIRQ 300
           LPWWEDATV FMLSGGY+V  QIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNA IRQ
Sbjct: 241 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 300

Query: 301 IPECGHLPHVEKPSFVAKLIMQFVHEDSIKEVQYVNS 338
           IPECGHLPHVEKP+FVAKLI QFVHEDS KEV YVN+
Sbjct: 301 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEVPYVNN 337

[Close]

BLAST of MS001008 vs. ExPASy Swiss-Prot
Match: O05235 (Uncharacterized hydrolase YugF OS=Bacillus subtilis (strain 168) OX=224308 GN=yugF PE=3 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 2.1e-15
Identity = 65/249 (26.10%), Postives = 118/249 (47.39%), Query Frame = 0


Query: 83  VVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGFSDLERLPPCDVTSKRDHLYQLW 142
           +V +HGF SS   +R   PLL +   +  A+D+  +G S+  R       +    +  + 
Sbjct: 30  LVCVHGFLSSAFSFRKVIPLLRDK-YDIIALDLPPFGQSEKSRTFIYTYQNLAKLVIGIL 89

Query: 143 KSYIKRPMVLVGPSLGAAVAIDFAVNHPEAVDRLVLIDASVYAEGTGNLATLPRLIAYAG 202
           +    +  VLVG S+G  +++  A+  PE   ++VL+ +S Y + +         I Y  
Sbjct: 90  EHLQVKQAVLVGHSMGGQISLSAALQKPELFSKVVLLCSSGYLKRSHPTIIFGTHIPYFH 149

Query: 203 VFLLKSVPLR-VYVNLL-AFTDISFSTNLDWSNIGRLHCLLPWWEDATVKFML-----SG 262
           +++ + +    V  NLL    D S          GR     P+ ++   K M        
Sbjct: 150 LYIKRWLSKEGVMKNLLNVVHDKSLIDEEMIDGYGR-----PFQDEQIFKAMTRFIRHRE 209

Query: 263 GYDVGGQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAVIRQIPECGHLPHVEKPSF 322
           G     Q++K+ +  L+IWGE+D+I+  ++  RLH +LPN+V+  + + GHL   E+P  
Sbjct: 210 GDLEPEQLKKMNKPALLIWGEEDRIVPMEIGKRLHADLPNSVLYSLGQTGHLVPEERPEL 269

Query: 323 VAKLIMQFV 325
           +++ I  F+
Sbjct: 270 ISEHIADFI 272

[Close]

BLAST of MS001008 vs. ExPASy Swiss-Prot
Match: Q476M7 (2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) OX=264198 GN=mhpC PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 5.0e-12
Identity = 68/262 (25.95%), Postives = 111/262 (42.37%), Query Frame = 0


Query: 80  ASPVVLLHGFDSSCLEW----RYTYPLLEEAGLETWAVDILGWGFSDLERLPPCDVTSKR 139
           A  VV+LHG       W    R   PL+  AG     VD  GWG SD    P  +  S+ 
Sbjct: 37  AETVVMLHGSGPGATGWANFNRNVEPLV-AAGYRVLLVDCPGWGKSD----PVVNAGSRS 96

Query: 140 DHLYQLWKSYIKRPMV----LVGPSLGAAVAIDFAVNHPEAVDRLVLIDA-----SVYAE 199
           +   ++ K  +    +    ++G S+G   A+ FA+ +P+ V +LVL+       S+YA 
Sbjct: 97  ELNGRVLKGVLDELDIERVHILGNSMGGHSAVAFALANPQRVGKLVLMGGGTGGPSLYAP 156

Query: 200 -GTGNLATLPRLIAYAGVFLLKSVPLRVYVNLLAFTDISFSTNLDWSNIGRLHCLLPWWE 259
             T  +  L  L     +  LK +      +  + TD      LD     R H       
Sbjct: 157 MPTEGIKLLNGLYREPSIENLKRMMNVFVYDASSLTDDLMQARLDNMLARRDHL------ 216

Query: 260 DATVKFMLSGG---YDVGGQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAVIRQIP 319
           +  VK + +      D G ++ ++   TL+IWG DD+ +   + +RL   +PNA +    
Sbjct: 217 ENFVKSLAANPKQFTDYGSRLGEITAPTLVIWGRDDRFVPMDVGLRLIAGIPNAQMHIFN 276

Query: 320 ECGHLPHVEKPSFVAKLIMQFV 325
            CGH    E      ++++ F+
Sbjct: 277 RCGHWAQWEHAKAFNRMVVDFL 287

[Close]

BLAST of MS001008 vs. ExPASy Swiss-Prot
Match: O31581 (AB hydrolase superfamily protein YfhM OS=Bacillus subtilis (strain 168) OX=224308 GN=yfhM PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 5.0e-12
Identity = 67/265 (25.28%), Postives = 108/265 (40.75%), Query Frame = 0


Query: 83  VVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGFSDL-ERLPPCDVTSKRDHLYQL 142
           +VLLHGF      W+     L +AG    A D  G+  SD  E +    + + RD +  L
Sbjct: 29  IVLLHGFPEFWYGWKNQIKPLVDAGYRVIAPDQRGYNLSDKPEGIDSYRIDTLRDDIIGL 88

Query: 143 WKSYIKRPMVLVGPSLGAAVAIDFAVNHPEAVDRLVLID-ASVYAEGTGNLATLPRLI-- 202
              +     +++G   G AVA   A   PE +++L+ I+    +   T      P+ +  
Sbjct: 89  ITQFTDEKAIVIGHDWGGAVAWHLASTRPEYLEKLIAINIPHPHVMKTVTPLYPPQWLKS 148

Query: 203 AYAGVFLLKSVP-----------LRVYVNL----LAFTDISFSTNLD-WSNIGRLHCLLP 262
           +Y   F L  +P           L   + L      FT    S   + W   G L  +L 
Sbjct: 149 SYIAYFQLPDIPEASLRENDYDTLDKAIGLSDRPALFTSEDVSRYKEAWKQPGALTAMLN 208

Query: 263 WWEDATVKFMLSGGYDVGGQIEKVKQKTL---IIWGEDDQIISNKLAVRLHCELPNAVIR 322
           W+               G   EK   +T+   +IWG +D+ +S KLA       PN  + 
Sbjct: 209 WYR----------ALRKGSLAEKPSYETVPYRMIWGMEDRFLSRKLAKETERHCPNGHLI 268

Query: 323 QIPECGHLPHVEKPSFVAKLIMQFV 325
            + E  H  + EKP+ V +LI++++
Sbjct: 269 FVDEASHWINHEKPAIVNQLILEYL 283

[Close]

BLAST of MS001008 vs. ExPASy Swiss-Prot
Match: B2JQW2 (2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Paraburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815) OX=391038 GN=mhpC PE=3 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 2.5e-11
Identity = 62/258 (24.03%), Postives = 106/258 (41.09%), Query Frame = 0


Query: 83  VVLLHGFDSSCLEWRYTYPLLE---EAGLETWAVDILGWGFSDLERLPPCDVTSKRDHLY 142
           +VLLHG       W   Y  ++    AG     VD  GWG SD          S+ D   
Sbjct: 39  LVLLHGSGPGASGWANFYRNVDAFANAGYRVILVDCPGWGKSD----SIVCTGSRSDLNA 98

Query: 143 QLWKSYIKRPMV----LVGPSLGAAVAIDFAVNHPEAVDRLVLIDASVYAEGTGNLATLP 202
           ++ K  +    +    LVG S+G   A+ FA+++PE V +LVL+       G      +P
Sbjct: 99  RVLKGVLDTLGIERAHLVGNSMGGHSAVAFALSYPERVGKLVLMGGG--TGGPSQFVPMP 158

Query: 203 RLIAYAGVFLLKSV-------PLRVYVNLLAFTDISFSTNLDWSNIGRLHCLLPWWEDAT 262
                 G+ LL+ +        L+  +N+  +   + +  L  + +  +       E+  
Sbjct: 159 T----EGIKLLQGLYRDPTLENLKKMLNVFVYDASTMTEELMQTRLDNMLARRDHLENFV 218

Query: 263 VKFMLSGGY--DVGGQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAVIRQIPECGH 322
                +     D G ++ ++K   L+IWG DD+ +   + +RL   LPNA       CGH
Sbjct: 219 KSLTANPKQFPDYGHRLNEIKAPALVIWGRDDRFVPLDVGLRLVWGLPNAEFHVFGRCGH 278

Query: 323 LPHVEKPSFVAKLIMQFV 325
               E      ++++ F+
Sbjct: 279 WAQWEHAERFNQMLLDFL 286

[Close]

BLAST of MS001008 vs. ExPASy Swiss-Prot
Match: A4JPX5 (2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Burkholderia vietnamiensis (strain G4 / LMG 22486) OX=269482 GN=mhpC PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 3.2e-11
Identity = 65/262 (24.81%), Postives = 111/262 (42.37%), Query Frame = 0


Query: 83  VVLLHGFDSSCLEWRYTYPLLE---EAGLETWAVDILGWGFSDLERLPPCDVTSKRDHLY 142
           +VLLHG       W   +  ++    AG     VD  GWG SD         T  R  L 
Sbjct: 39  LVLLHGSGPGATGWANFHRNVDAFANAGYRVILVDCPGWGKSD-----SIVCTGSRSDLN 98

Query: 143 QLWKSYIKRPM-----VLVGPSLGAAVAIDFAVNHPEAVDRLVLIDASVYAEGTGNLATL 202
               + +   +      LVG S+G   A+ FA+++PE V +LVL+       G      +
Sbjct: 99  ARVLAGVLDTLGIGRAHLVGNSMGGHSAVAFALSYPERVGKLVLMGGG--TGGPSQFVPM 158

Query: 203 PRLIAYAGVFLLKSV-------PLRVYVNLLAF-----TDISFSTNLDWSNIGRLHCLLP 262
           P      G+ LL+++        L+  +N+  +     T+    T L+ + +GR   L  
Sbjct: 159 PT----EGIKLLQALYRDPTLENLKKMLNVFVYDASTMTEELMQTRLE-NMLGRRDHLEN 218

Query: 263 WWEDATVKFMLSGGYDVGGQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAVIRQIP 322
           + +  T        Y  G ++ ++K   L+IWG DD+ +   + +RL   +PNA +    
Sbjct: 219 FVKSLTANPKQFPDY--GHRLSEIKAPALVIWGRDDRFVPMDVGLRLVWNMPNADLHVFG 278

Query: 323 ECGHLPHVEKPSFVAKLIMQFV 325
            CGH    E      +++++F+
Sbjct: 279 RCGHWAQWEHAERFNRMVLEFL 286

[Close]

BLAST of MS001008 vs. ExPASy TrEMBL
Match: A0A6J1BTG8 (uncharacterized protein LOC111004671 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111004671 PE=4 SV=1)

HSP 1 Score: 677.2 bits (1746), Expect = 3.6e-191
Identity = 330/335 (98.51%), Postives = 331/335 (98.81%), Query Frame = 0


Query: 1   MLALTVASSLGSAPLHTKWKKPKRLGFTINEFPSFLPKEVENIKDQFARKLATRIERLPV 60
           MLALTVASSLGSAPLHTKWKKPKRLGFTINEFPSFLPKEVENIKDQFARKLATRIERLPV
Sbjct: 1   MLALTVASSLGSAPLHTKWKKPKRLGFTINEFPSFLPKEVENIKDQFARKLATRIERLPV 60

Query: 61  SFSDTCIMSSFVKPSIQSKASPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGF 120
           SFSDTCIMSSFVKPSIQSKASPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGF
Sbjct: 61  SFSDTCIMSSFVKPSIQSKASPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGF 120

Query: 121 SDLERLPPCDVTSKRDHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNHPEAVDRLVLID 180
           SDLERLPPCDVTSKRDHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNHPEAVDRLVLID
Sbjct: 121 SDLERLPPCDVTSKRDHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNHPEAVDRLVLID 180

Query: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSVPLRVYVNLLAFTDISFSTNLDWSNIGRLHCL 240
           ASVYAEGTGNLATLPRLIAYAGVFLLKSVPLRVYVNLLAFTDISFSTNLDWSNIGRLHCL
Sbjct: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSVPLRVYVNLLAFTDISFSTNLDWSNIGRLHCL 240

Query: 241 LPWWEDATVKFMLSGGYDVGGQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAVIRQ 300
           LPWWEDATVKFM SGGYDVGGQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAVIRQ
Sbjct: 241 LPWWEDATVKFMSSGGYDVGGQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAVIRQ 300

Query: 301 IPECGHLPHVEKPSFVAKLIMQFVHEDSIKEVQYV 336
           IPECGHLPHVEKPSFVAKLIMQFVHEDS KEV  +
Sbjct: 301 IPECGHLPHVEKPSFVAKLIMQFVHEDSRKEVDSI 335

[Close]

BLAST of MS001008 vs. ExPASy TrEMBL
Match: A0A6J1BQC3 (uncharacterized protein LOC111004671 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111004671 PE=4 SV=1)

HSP 1 Score: 675.2 bits (1741), Expect = 1.4e-190
Identity = 329/331 (99.40%), Postives = 329/331 (99.40%), Query Frame = 0


Query: 1   MLALTVASSLGSAPLHTKWKKPKRLGFTINEFPSFLPKEVENIKDQFARKLATRIERLPV 60
           MLALTVASSLGSAPLHTKWKKPKRLGFTINEFPSFLPKEVENIKDQFARKLATRIERLPV
Sbjct: 1   MLALTVASSLGSAPLHTKWKKPKRLGFTINEFPSFLPKEVENIKDQFARKLATRIERLPV 60

Query: 61  SFSDTCIMSSFVKPSIQSKASPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGF 120
           SFSDTCIMSSFVKPSIQSKASPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGF
Sbjct: 61  SFSDTCIMSSFVKPSIQSKASPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGF 120

Query: 121 SDLERLPPCDVTSKRDHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNHPEAVDRLVLID 180
           SDLERLPPCDVTSKRDHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNHPEAVDRLVLID
Sbjct: 121 SDLERLPPCDVTSKRDHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNHPEAVDRLVLID 180

Query: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSVPLRVYVNLLAFTDISFSTNLDWSNIGRLHCL 240
           ASVYAEGTGNLATLPRLIAYAGVFLLKSVPLRVYVNLLAFTDISFSTNLDWSNIGRLHCL
Sbjct: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSVPLRVYVNLLAFTDISFSTNLDWSNIGRLHCL 240

Query: 241 LPWWEDATVKFMLSGGYDVGGQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAVIRQ 300
           LPWWEDATVKFM SGGYDVGGQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAVIRQ
Sbjct: 241 LPWWEDATVKFMSSGGYDVGGQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAVIRQ 300

Query: 301 IPECGHLPHVEKPSFVAKLIMQFVHEDSIKE 332
           IPECGHLPHVEKPSFVAKLIMQFVHEDS KE
Sbjct: 301 IPECGHLPHVEKPSFVAKLIMQFVHEDSRKE 331

[Close]

BLAST of MS001008 vs. ExPASy TrEMBL
Match: A0A6J1HBV7 (uncharacterized protein LOC111462669 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462669 PE=4 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 2.4e-171
Identity = 297/337 (88.13%), Postives = 313/337 (92.88%), Query Frame = 0


Query: 1   MLALTVASSLGSAPLHTKWKKPKRLGFTINEFPSFLPKEVENIKDQFARKLATRIERLPV 60
           ML+L VA+S  SAP   +WKKPKRLG  +N+FPSFLPKEV+NIKDQFAR+LATRIERLPV
Sbjct: 1   MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV 60

Query: 61  SFSDTCIMSSFVKPSIQSKASPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGF 120
           SFSD+C MSS VKPSIQSK  PVVLLHGFDSSCLEWRYTYPLLEEAG ETWAVDILGWGF
Sbjct: 61  SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 120

Query: 121 SDLERLPPCDVTSKRDHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNHPEAVDRLVLID 180
           SDLERLPPCDVTSKR HLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVN+PEAVDRLVLID
Sbjct: 121 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID 180

Query: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSVPLRVYVNLLAFTDISFSTNLDWSNIGRLHCL 240
           ASVYAEGTGNLATLPRLIAYAGVFLLKS+PLR+YVN+LAFT IS ST+LDW NIGRLHCL
Sbjct: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 240

Query: 241 LPWWEDATVKFMLSGGYDVGGQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAVIRQ 300
           LPWWEDATV FMLSGGY+V  QIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNA IRQ
Sbjct: 241 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 300

Query: 301 IPECGHLPHVEKPSFVAKLIMQFVHEDSIKEVQYVNS 338
           IPECGHLPHVEKP+FVAKLI QFVHEDS KEV YVN+
Sbjct: 301 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEVPYVNN 337

[Close]

BLAST of MS001008 vs. ExPASy TrEMBL
Match: A0A6J1KAS9 (uncharacterized protein LOC111492171 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492171 PE=4 SV=1)

HSP 1 Score: 606.7 bits (1563), Expect = 6.0e-170
Identity = 295/332 (88.86%), Postives = 310/332 (93.37%), Query Frame = 0


Query: 1   MLALTVASSLGSAPLHTKWKKPKRLGFTINEFPSFLPKEVENIKDQFARKLATRIERLPV 60
           ML+L VA+S  SAP   +WKKPKRLG  IN+FPSFLPKEV+NIKDQFAR+LATRIERLPV
Sbjct: 1   MLSLAVAASWCSAPPLMQWKKPKRLGVIINKFPSFLPKEVDNIKDQFARELATRIERLPV 60

Query: 61  SFSDTCIMSSFVKPSIQSKASPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGF 120
           SFSD+CIMSS VKPSIQSK  PVVLLHGFDSSCLEWRYTYPLLEEAG ETWAVDILGWGF
Sbjct: 61  SFSDSCIMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 120

Query: 121 SDLERLPPCDVTSKRDHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNHPEAVDRLVLID 180
           SDLERLPPCDVTSKR HLYQLWKSYIKRPMV+VGPSLGAAVAIDFAVN+PEAVDRLVLID
Sbjct: 121 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVMVGPSLGAAVAIDFAVNYPEAVDRLVLID 180

Query: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSVPLRVYVNLLAFTDISFSTNLDWSNIGRLHCL 240
           ASVYAEGTGNLATLPRLIAYAGVFLLKS+PLR+YVN+LAFT IS ST+LDW NIGRLHCL
Sbjct: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 240

Query: 241 LPWWEDATVKFMLSGGYDVGGQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAVIRQ 300
           LPWWEDATV FMLSGGY+V  QIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNA IRQ
Sbjct: 241 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 300

Query: 301 IPECGHLPHVEKPSFVAKLIMQFVHEDSIKEV 333
           IPECGHLPHVEKP+FVAKLI QFVHEDS KEV
Sbjct: 301 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEV 332

[Close]

BLAST of MS001008 vs. ExPASy TrEMBL
Match: A0A6J1KD64 (uncharacterized protein LOC111492171 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492171 PE=4 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 1.3e-169
Identity = 294/332 (88.55%), Postives = 310/332 (93.37%), Query Frame = 0


Query: 1   MLALTVASSLGSAPLHTKWKKPKRLGFTINEFPSFLPKEVENIKDQFARKLATRIERLPV 60
           ML+L VA+S  SAP   +WKKPKRLG  IN+FPSFLPKEV+NIKDQFAR+LATRIERLPV
Sbjct: 1   MLSLAVAASWCSAPPLMQWKKPKRLGVIINKFPSFLPKEVDNIKDQFARELATRIERLPV 60

Query: 61  SFSDTCIMSSFVKPSIQSKASPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGF 120
           SFSD+CIMSS VKPSIQSK  PVVLLHGFDSSCLEWRYTYPLLEEAG ETWAVDILGWGF
Sbjct: 61  SFSDSCIMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 120

Query: 121 SDLERLPPCDVTSKRDHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNHPEAVDRLVLID 180
           SDLERLPPCDVTSKR HLYQLWKSYIKRPMV+VGPSLGAAVAIDFAVN+PEAVDRLVLID
Sbjct: 121 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVMVGPSLGAAVAIDFAVNYPEAVDRLVLID 180

Query: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSVPLRVYVNLLAFTDISFSTNLDWSNIGRLHCL 240
           ASVYAEGTGNLATLPRLIAYAGVFLLKS+PLR+YVN+LAFT IS ST+LDW NIGRLHCL
Sbjct: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 240

Query: 241 LPWWEDATVKFMLSGGYDVGGQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAVIRQ 300
           LPWWEDATV FMLSGGY+V  QIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNA IRQ
Sbjct: 241 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 300

Query: 301 IPECGHLPHVEKPSFVAKLIMQFVHEDSIKEV 333
           IPECGHLPHVEKP+FVAKLI QFVHEDS KE+
Sbjct: 301 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEL 332

[Close]

BLAST of MS001008 vs. TAIR 10
Match: AT1G13820.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 468.0 bits (1203), Expect = 6.4e-132
Identity = 228/339 (67.26%), Postives = 268/339 (79.06%), Query Frame = 0


Query: 1   MLALTVASSLGSAPLHTKWKKPKRLGFTINEFPSFLPKEVENIKDQFARKLATRIERLP- 60
           ML+   A+++    L  +    +    T  +FP+FLP +VE IKD FA KLA RIERLP 
Sbjct: 1   MLSTVTATAVSRPILKVECTIKRTFKVTAEKFPTFLPTDVEKIKDTFALKLAARIERLPV 60

Query: 61  -VSFSDTCIMSSFVKPSIQSKASPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGW 120
            VSF +  IMSS V P ++++ +PVVLLHGFDSSCLEWRYTYPLLEEAGLETWA DILGW
Sbjct: 61  SVSFREDSIMSSCVTPLMRNETTPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAFDILGW 120

Query: 121 GFSDLERLPPCDVTSKRDHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNHPEAVDRLVL 180
           GFSDL++LPPCDV SKR+H Y+ WKS+IKRP+VLVGPSLGAAVAID AVNHPEAV+ LVL
Sbjct: 121 GFSDLDKLPPCDVASKREHFYKFWKSHIKRPVVLVGPSLGAAVAIDIAVNHPEAVESLVL 180

Query: 181 IDASVYAEGTGNLATLPRLIAYAGVFLLKSVPLRVYVNLLAFTDISFSTNLDWSNIGRLH 240
           +DASVYAEGTGNLATLP+  AYAGV+LLKS+PLR+YVN + F  IS  T+ DW+ IGRLH
Sbjct: 181 MDASVYAEGTGNLATLPKAAAYAGVYLLKSIPLRLYVNFICFNGISLETSWDWTKIGRLH 240

Query: 241 CLLPWWEDATVKFMLSGGYDVGGQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAVI 300
           CL PWWEDATV FM SGGY+V   I+KV QKTLI+WGEDDQIISNKLA RLH EL NA +
Sbjct: 241 CLYPWWEDATVSFMTSGGYNVTSLIKKVSQKTLILWGEDDQIISNKLAWRLHGELSNARV 300

Query: 301 RQIPECGHLPHVEKPSFVAKLIMQFVHED-SIKEVQYVN 337
           +QI  CGHLPHVEKP+ V KLI +FV E    KEV+ ++
Sbjct: 301 KQISNCGHLPHVEKPAAVTKLIAEFVRETCRCKEVESIS 339

[Close]

BLAST of MS001008 vs. TAIR 10
Match: AT5G39220.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 406.8 bits (1044), Expect = 1.8e-113
Identity = 189/297 (63.64%), Postives = 232/297 (78.11%), Query Frame = 0


Query: 32  FPSFLPKEVENIKDQFARKLATRIERLPVSFS----DTCIMSSFVKPSIQ-SKASPVVLL 91
           FP+FLPKE++NIKD FAR LA RI R+PV          +MSS +KP +Q    SPVVLL
Sbjct: 28  FPTFLPKEIQNIKDPFARALAQRIVRIPVPLQMGNFRGSVMSSCIKPLVQLHDKSPVVLL 87

Query: 92  HGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGFSDLERLPPCDVTSKRDHLYQLWKSYI 151
           H FDSSCLEWR TYPLLE+A LETWA+D+LGWGFSDLE+LPPCD  SKR HL++LWK+YI
Sbjct: 88  HCFDSSCLEWRRTYPLLEQACLETWAIDVLGWGFSDLEKLPPCDAASKRHHLFELWKTYI 147

Query: 152 KRPMVLVGPSLGAAVAIDFAVNHPEAVDRLVLIDASVYAEGTGNLATLPRLIAYAGVFLL 211
           KRPM+LVGPSLGA VA+DF   +PEAVD+LVLI+A+ Y+EGTG L  LP+ IAYAGV LL
Sbjct: 148 KRPMILVGPSLGATVAVDFTATYPEAVDKLVLINANAYSEGTGRLKELPKSIAYAGVKLL 207

Query: 212 KSVPLRVYVNLLAFTDISFSTNLDWSNIGRLHCLLPWWEDATVKFMLSGGYDVGGQIEKV 271
           KS PLR+  N+LAF     S N+DW+NIGRLHC +PWWEDA V FM+SGGY+V   I+ +
Sbjct: 208 KSFPLRLLANVLAFCSSPLSENIDWTNIGRLHCQMPWWEDAMVDFMISGGYNVASHIKHI 267

Query: 272 KQKTLIIWGEDDQIISNKLAVRLHCELPNAVIRQIPECGHLPHVEKPSFVAKLIMQF 324
             KTL++  E+DQI+SN+L+V+L CEL NAV+R++P+ GHLPHVE P  + KLI  F
Sbjct: 268 DHKTLVVCSENDQIVSNQLSVKLLCELANAVLREVPDSGHLPHVENPKHIVKLISDF 324

[Close]

BLAST of MS001008 vs. TAIR 10
Match: AT4G36530.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 87.0 bits (214), Expect = 3.1e-17
Identity = 73/277 (26.35%), Postives = 123/277 (44.40%), Query Frame = 0


Query: 76  IQSKASPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGFSDLERLPPCDVTSKR 135
           +Q + SP+VL+HGF +S   WRY  P L +   + +A+D+LG+G+SD + L   D     
Sbjct: 38  VQGEGSPLVLIHGFGASVFHWRYNIPELAKK-YKVYALDLLGFGWSD-KALIEYDAMVWT 97

Query: 136 DHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNHPEAVDRLVLIDAS--VYAEGTGNLAT 195
           D +    K  +K P V+VG SLG   A+  AV  PE V  + L++++    AE       
Sbjct: 98  DQVIDFMKEVVKEPAVVVGNSLGGFTALSVAVGLPEQVTGVALLNSAGQFAAESRKREEA 157

Query: 196 LPRLIAYAGVFLLKSVPLRVYVNLLAF-------------TDISFSTNLD---------- 255
              +I    V  LK +  RV +  L +             +    STN+D          
Sbjct: 158 DETVITKFIVKPLKEIFQRVVLGFLFWQAKQPSRIESVLKSVYIDSTNVDDYLVESISKP 217

Query: 256 --WSNIGRLHCLLPWWEDATVKFMLS-GGYDVGGQIEKVKQKTLIIWGEDDQIISNKLAV 315
               N G ++  L        +F+ +   Y +   + K+    L++WG+ D  +    A 
Sbjct: 218 ATDPNAGEVYYRL------MTRFLTNQSRYTLDSVLSKMTCPLLLVWGDLDPWVGPAKAE 277

Query: 316 RLHCELPNAVIRQIPECGHLPHVEKPSFVAKLIMQFV 325
           ++     N+ +  + + GH PH E P  V K ++ ++
Sbjct: 278 KIKAFYSNSSLVHL-QAGHCPHDEVPEAVNKALLDWL 305

[Close]

BLAST of MS001008 vs. TAIR 10
Match: AT4G36530.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 87.0 bits (214), Expect = 3.1e-17
Identity = 73/277 (26.35%), Postives = 123/277 (44.40%), Query Frame = 0


Query: 76  IQSKASPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGFSDLERLPPCDVTSKR 135
           +Q + SP+VL+HGF +S   WRY  P L +   + +A+D+LG+G+SD + L   D     
Sbjct: 95  VQGEGSPLVLIHGFGASVFHWRYNIPELAKK-YKVYALDLLGFGWSD-KALIEYDAMVWT 154

Query: 136 DHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNHPEAVDRLVLIDAS--VYAEGTGNLAT 195
           D +    K  +K P V+VG SLG   A+  AV  PE V  + L++++    AE       
Sbjct: 155 DQVIDFMKEVVKEPAVVVGNSLGGFTALSVAVGLPEQVTGVALLNSAGQFAAESRKREEA 214

Query: 196 LPRLIAYAGVFLLKSVPLRVYVNLLAF-------------TDISFSTNLD---------- 255
              +I    V  LK +  RV +  L +             +    STN+D          
Sbjct: 215 DETVITKFIVKPLKEIFQRVVLGFLFWQAKQPSRIESVLKSVYIDSTNVDDYLVESISKP 274

Query: 256 --WSNIGRLHCLLPWWEDATVKFMLS-GGYDVGGQIEKVKQKTLIIWGEDDQIISNKLAV 315
               N G ++  L        +F+ +   Y +   + K+    L++WG+ D  +    A 
Sbjct: 275 ATDPNAGEVYYRL------MTRFLTNQSRYTLDSVLSKMTCPLLLVWGDLDPWVGPAKAE 334

Query: 316 RLHCELPNAVIRQIPECGHLPHVEKPSFVAKLIMQFV 325
           ++     N+ +  + + GH PH E P  V K ++ ++
Sbjct: 335 KIKAFYSNSSLVHL-QAGHCPHDEVPEAVNKALLDWL 362

[Close]

BLAST of MS001008 vs. TAIR 10
Match: AT5G09430.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 68.6 bits (166), Expect = 1.1e-11
Identity = 70/261 (26.82%), Postives = 118/261 (45.21%), Query Frame = 0


Query: 74  PSIQSKASP-VVLLHGFDSSCLEWRYTYPLLEEAG-LETWAVDILGWGFSDLERLPPCDV 133
           P   +++ P ++LLHGF ++ + W+Y   L    G    +  D+L +G S     P    
Sbjct: 53  PKSPNRSKPNLLLLHGFGANAM-WQYGEHLRAFTGRFNVYVPDLLFFGLSSTSE-PNRTE 112

Query: 134 TSKRDHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNHPEAVDRLVLIDASVYAEG---T 193
           + +   L +L +++  + M +VG S G  V    A   PE V++LVL  A V  E     
Sbjct: 113 SFQARCLMRLMEAHGVQRMNIVGISYGGFVGYSLAAQFPENVEKLVLCCAGVCLEEKDME 172

Query: 194 GNLATLPRLIAYAGVFLLKSVPLRVYVNLLAFTDISFSTNLD----WSNIGRLHCLLPWW 253
             L  +P L    G+ L+   P ++   L+ F+ +     +     W  I  +       
Sbjct: 173 DGLFKVPNLEEATGI-LIPQTPEKL-KELIRFSFVKPIKGVPSFFLWDFIDVMCTEFVEE 232

Query: 254 EDATVKFMLSGGYDVGGQIEKVKQKTLIIWGEDDQIISNKLAVRLHCEL-PNAVIRQIPE 313
           +   +K +L         + ++KQK+LIIWGE+DQI   +L  RL   +  +A I  I +
Sbjct: 233 KRDLIKSILKDRR--LSDLPRIKQKSLIIWGEEDQIFPLELGYRLKRHIGESAEIVVIKK 292

Query: 314 CGHLPHVEKPSFVAKLIMQFV 325
            GH  ++EK     K +  F+
Sbjct: 293 AGHAVNLEKSKEFVKHLKSFL 307

The following BLAST results are available for this feature:

BLAST of MS001008 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022131473.1	7.5e-191	98.51	uncharacterized protein LOC111004671 isoform X1 [Momordica charantia]	[more]
XP_022131474.1	2.8e-190	99.40	uncharacterized protein LOC111004671 isoform X2 [Momordica charantia]	[more]
XP_038884275.1	5.9e-172	88.13	uncharacterized hydrolase YugF isoform X2 [Benincasa hispida]	[more]
KAG7029571.1	5.0e-171	88.13	yugF, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022962117.1	5.0e-171	88.13	uncharacterized protein LOC111462669 isoform X1 [Cucurbita moschata]	[more]

BLAST of MS001008 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O05235	2.1e-15	26.10	Uncharacterized hydrolase YugF OS=Bacillus subtilis (strain 168) OX=224308 GN=yu...	[more]
Q476M7	5.0e-12	25.95	2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Cupr...	[more]
O31581	5.0e-12	25.28	AB hydrolase superfamily protein YfhM OS=Bacillus subtilis (strain 168) OX=22430...	[more]
B2JQW2	2.5e-11	24.03	2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Para...	[more]
A4JPX5	3.2e-11	24.81	2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Burk...	[more]

BLAST of MS001008 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BTG8	3.6e-191	98.51	uncharacterized protein LOC111004671 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1BQC3	1.4e-190	99.40	uncharacterized protein LOC111004671 isoform X2 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1HBV7	2.4e-171	88.13	uncharacterized protein LOC111462669 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1KAS9	6.0e-170	88.86	uncharacterized protein LOC111492171 isoform X2 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1KD64	1.3e-169	88.55	uncharacterized protein LOC111492171 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]

BLAST of MS001008 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G13820.1	6.4e-132	67.26	alpha/beta-Hydrolases superfamily protein	[more]
AT5G39220.1	1.8e-113	63.64	alpha/beta-Hydrolases superfamily protein	[more]
AT4G36530.1	3.1e-17	26.35	alpha/beta-Hydrolases superfamily protein	[more]
AT4G36530.2	3.1e-17	26.35	alpha/beta-Hydrolases superfamily protein	[more]
AT5G09430.1	1.1e-11	26.82	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000639	Epoxide hydrolase-like	PRINTS	PR00412	EPOXHYDRLASE	coord: 166..179 score: 28.9 coord: 301..323 score: 27.67 coord: 87..105 score: 36.84 coord: 107..122 score: 37.22
IPR000073	Alpha/beta hydrolase fold-1	PRINTS	PR00111	ABHYDROLASE	coord: 107..122 score: 33.84 coord: 166..179 score: 42.61 coord: 152..165 score: 42.86 coord: 270..284 score: 41.84
IPR000073	Alpha/beta hydrolase fold-1	PFAM	PF12697	Abhydrolase_6	coord: 83..318 e-value: 2.2E-16 score: 61.1
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 72..331 e-value: 2.9E-43 score: 150.7
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 81..324
None	No IPR available	PANTHER	PTHR43689	HYDROLASE	coord: 25..331
None	No IPR available	PANTHER	PTHR43689:SF22	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 25..331

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS001008.1	MS001008.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003824	catalytic activity