MS000611 (gene) Bitter gourd (TR) v1

BLAST of MS000611 vs. NCBI nr
Match: XP_022150430.1 (MACPF domain-containing protein At1g14780-like [Momordica charantia])

HSP 1 Score: 1236.5 bits (3198), Expect = 0.0e+00
Identity = 605/606 (99.83%), Postives = 605/606 (99.83%), Query Frame = 0

Query: 53  MPASEKPVEIRALECLGRGFDLTNDFRLKFVKGYGGGGRRLVVVDDSNRRDMVFPDGAVV 112
           MPASEKPVEIRALECLGRGFDLTNDFRLKFVKGYGGGGRRLVVVDDSNRRDMVFPDGAVV
Sbjct: 1   MPASEKPVEIRALECLGRGFDLTNDFRLKFVKGYGGGGRRLVVVDDSNRRDMVFPDGAVV 60

Query: 113 RGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQKSSIQGKVPSGYFNSMFGLSGDWFHDA 172
           RGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQKSSIQGKVPSGYFNSMFGLSGDWFHDA
Sbjct: 61  RGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQKSSIQGKVPSGYFNSMFGLSGDWFHDA 120

Query: 173 ADANHLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSSWDPASLSRFIQTYGTHIIVGMA 232
           ADANHLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSSWDPASLSRFIQTYGTHIIVGMA
Sbjct: 121 ADANHLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSSWDPASLSRFIQTYGTHIIVGMA 180

Query: 233 IGGQDLISVRQRPSSPIPPAELRRNLEDLGDVLFSDRRSPSALLKRDVKVPEIFTRILQS 292
           IGGQDLISVRQRPSSPIPPAELRRNLEDLGDVLFSDRRSPSALLKRDVKVPEIFTRILQS
Sbjct: 181 IGGQDLISVRQRPSSPIPPAELRRNLEDLGDVLFSDRRSPSALLKRDVKVPEIFTRILQS 240

Query: 293 SSIQLASISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSSKPEVILFKFVPITSLLTG 352
           SSIQLASISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSSKPEVILFKFVPITSLLTG
Sbjct: 241 SSIQLASISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSSKPEVILFKFVPITSLLTG 300

Query: 353 VAGSGYLSHAINLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQRKKATCPTLQFA 412
           VAGSGYLSHAINLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQRKKATCPTLQFA
Sbjct: 301 VAGSGYLSHAINLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQRKKATCPTLQFA 360

Query: 413 FMSPRMYIASTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMASLMSSSDVAS 472
           FMSPRMYIASTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLP VMASLMSSSDVAS
Sbjct: 361 FMSPRMYIASTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPTVMASLMSSSDVAS 420

Query: 473 RCQWRGSDDGDCNGQFLEPVKWKRYSNVCTSAVRHDPNWLQQDGNGVYIVTGAQIISKGK 532
           RCQWRGSDDGDCNGQFLEPVKWKRYSNVCTSAVRHDPNWLQQDGNGVYIVTGAQIISKGK
Sbjct: 421 RCQWRGSDDGDCNGQFLEPVKWKRYSNVCTSAVRHDPNWLQQDGNGVYIVTGAQIISKGK 480

Query: 533 WPKTILHLRLQYTHMPNCTIRRTEWAAAPGASRKSTFLTNLSTTFSFTQRPAADKNKQVP 592
           WPKTILHLRLQYTHMPNCTIRRTEWAAAPGASRKSTFLTNLSTTFSFTQRPAADKNKQVP
Sbjct: 481 WPKTILHLRLQYTHMPNCTIRRTEWAAAPGASRKSTFLTNLSTTFSFTQRPAADKNKQVP 540

Query: 593 GALNSGVYGEAPPVPVRSKKLLKYVDTTEVVRGPHDSPGHWLVTAAKLVTESGKIGLNVK 652
           GALNSGVYGEAPPVPVRSKKLLKYVDTTEVVRGPHDSPGHWLVTAAKLVTESGKIGLNVK
Sbjct: 541 GALNSGVYGEAPPVPVRSKKLLKYVDTTEVVRGPHDSPGHWLVTAAKLVTESGKIGLNVK 600

Query: 653 FALLDY 659
           FALLDY
Sbjct: 601 FALLDY 606

BLAST of MS000611 vs. NCBI nr
Match: XP_038885599.1 (LOW QUALITY PROTEIN: MACPF domain-containing protein At4g24290-like [Benincasa hispida])

HSP 1 Score: 1160.6 bits (3001), Expect = 0.0e+00
Identity = 585/667 (87.71%), Postives = 607/667 (91.00%), Query Frame = 0

Query: 4   ILSGQIWGQVKFSDLYLLLLLHLL---LTHLNLIVLFVWLLRFLVDLISS--------SA 63
           ILSGQIW QVK  DLYLLLLL LL   LT LNLI  F W LRF VD  SS        SA
Sbjct: 4   ILSGQIWRQVKILDLYLLLLLLLLLPSLTFLNLIP-FAWFLRFFVDHXSSFADLAVGRSA 63

Query: 64  MPASEKPVEIRALECLGRGFDLTNDFRLKFVKGYGGGGRRLVVVDDSNRRDMVFPDGAVV 123
           M + EK VE+RAL+CLGRGFDLTNDFRLKFVKG G GGRRLVVVDD N+RDMVFPDGAVV
Sbjct: 64  MLSPEKSVEVRALDCLGRGFDLTNDFRLKFVKGNGVGGRRLVVVDDVNKRDMVFPDGAVV 123

Query: 124 RGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQKSSIQGKVPSGYFNSMFGLSGDWFHDA 183
           RGVSQDIRCDKGDRIRFKSDVLQFNQMSE LNQKSSIQGKVPSGYFNSMF LSGDWFHDA
Sbjct: 124 RGVSQDIRCDKGDRIRFKSDVLQFNQMSETLNQKSSIQGKVPSGYFNSMFDLSGDWFHDA 183

Query: 184 ADANHLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSSWDPASLSRFIQTYGTHIIVGMA 243
           ADAN+LAFDGYFIALYYLHLTASPLVLQDEVKKAVPS+WDPASLSRFIQTYGTHIIVG A
Sbjct: 184 ADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSNWDPASLSRFIQTYGTHIIVGTA 243

Query: 244 IGGQDLISVRQRPSSPIPPAELRRNLEDLGDVLFSDRRSPSAL-LKRDVKVPEIFTRILQ 303
           IGGQDLISVRQRPSSPIPPAELRR+LEDLGD+LFSD+RSPS L  KRD KVPE+FTRILQ
Sbjct: 244 IGGQDLISVRQRPSSPIPPAELRRHLEDLGDILFSDKRSPSTLQRKRDGKVPELFTRILQ 303

Query: 304 SSSIQLASISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSSKPEVILFKFVPITSLLT 363
           SSSIQL SISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSSKPEVILF+FVPITSLLT
Sbjct: 304 SSSIQLTSISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSSKPEVILFRFVPITSLLT 363

Query: 364 GVAGSGYLSHAINLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQRKKATCPTLQF 423
           GVAGSGYLSHAINLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQRKKATCPTLQF
Sbjct: 364 GVAGSGYLSHAINLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQRKKATCPTLQF 423

Query: 424 AFMSPRMYIASTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMASLMSSSDVA 483
            FMSPR YI  TQVTSS KPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMA LM +S+V+
Sbjct: 424 TFMSPRTYITPTQVTSSTKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLMGTSNVS 483

Query: 484 SRCQWRGSDDGDCNGQFLEPVKWKRYSNVCTSAVRHDPNWLQQDGNGVYIVTGAQIISKG 543
           SR +W GSDD D N QFLEP+KWK YSNVCTS VRHDPNWLQQDG+GVYIVTGAQ+ISKG
Sbjct: 484 SRSRWLGSDDSDSNSQFLEPIKWKSYSNVCTSVVRHDPNWLQQDGDGVYIVTGAQLISKG 543

Query: 544 KWPKTILHLRLQYTHMPNCTIRRTEWAAAPGASRKSTFLTNLSTTFSFTQRPAADKNKQV 603
           KWPKTILHLRLQ+TH+PNCTIRRTEWAAA   SRKSTFL NLSTTF+FTQR A DK KQV
Sbjct: 544 KWPKTILHLRLQFTHLPNCTIRRTEWAAARETSRKSTFLMNLSTTFTFTQRAAGDKKKQV 603

Query: 604 PGALNSGVYGEAPPVPVRSKKLLKYVDTTEVVRGPHDSPGHWLVTAAKLVTESGKIGLNV 659
           PGALNSGVYGEAPPVPVRSKKLLKYVDT EVVRGPHD+PGHWLVTAAKLVTE GKIGLNV
Sbjct: 604 PGALNSGVYGEAPPVPVRSKKLLKYVDTAEVVRGPHDAPGHWLVTAAKLVTEGGKIGLNV 663

BLAST of MS000611 vs. NCBI nr
Match: XP_004138354.2 (MACPF domain-containing protein At4g24290 [Cucumis sativus] >KGN45926.1 hypothetical protein Csa_005300 [Cucumis sativus])

HSP 1 Score: 1160.6 bits (3001), Expect = 0.0e+00
Identity = 584/675 (86.52%), Postives = 610/675 (90.37%), Query Frame = 0

Query: 1   MNRILSGQIWGQVKFSDLYLLLLLHLL-----LTHLNLIVLFVWLLRFLV---------- 60
           M RILSGQIWGQVKFSDLYLLLLL  L     LT LNLI  F W L F V          
Sbjct: 1   MIRILSGQIWGQVKFSDLYLLLLLLFLLLLPPLTFLNLIP-FAWFLPFFVVDHQLTSSFD 60

Query: 61  DL-ISSSAMPASEKPVEIRALECLGRGFDLTNDFRLKFVKGYGGGGRRLVVVDDSNRRDM 120
           DL +  SAM + EK VE+RAL+CLGRGFDLTNDFRLKFVKG GGGGRRLVVVDD N+RDM
Sbjct: 61  DLAVGGSAMLSPEKSVEVRALDCLGRGFDLTNDFRLKFVKGNGGGGRRLVVVDDVNKRDM 120

Query: 121 VFPDGAVVRGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQKSSIQGKVPSGYFNSMFGL 180
           VFPDGAVVRGVSQDIRCDKGDRIRFKSDVLQFNQMSE LNQKSSIQGKVPSGYFNSMF L
Sbjct: 121 VFPDGAVVRGVSQDIRCDKGDRIRFKSDVLQFNQMSETLNQKSSIQGKVPSGYFNSMFDL 180

Query: 181 SGDWFHDAADANHLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSSWDPASLSRFIQTYG 240
           SGDWFHDAADAN+LAFDGYFIALYYLHLTASPLVLQDEVKKAVPS+WDPASL+RFIQTYG
Sbjct: 181 SGDWFHDAADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSNWDPASLARFIQTYG 240

Query: 241 THIIVGMAIGGQDLISVRQRPSSPIPPAELRRNLEDLGDVLFSDRRSPSAL-LKRDVKVP 300
           THIIVG AIGGQDLISVRQRPSSPIPPAE+RR+LEDLGD+LFSD+RSPS L  +RD KVP
Sbjct: 241 THIIVGTAIGGQDLISVRQRPSSPIPPAEVRRHLEDLGDILFSDKRSPSTLQRRRDGKVP 300

Query: 301 EIFTRILQSSSIQLASISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSSKPEVILFKF 360
           EIFTRILQSSSIQL SISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSSKPEVILFKF
Sbjct: 301 EIFTRILQSSSIQLTSISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSSKPEVILFKF 360

Query: 361 VPITSLLTGVAGSGYLSHAINLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQRKK 420
           VPITSLLTGVAGSGYLSHAINLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQRKK
Sbjct: 361 VPITSLLTGVAGSGYLSHAINLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQRKK 420

Query: 421 ATCPTLQFAFMSPRMYIASTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMAS 480
           A+CPTLQF FMSPR Y+ STQVTSS KPLVGLRLYLEGK+SNWLALHVQHLTSLPKVMA 
Sbjct: 421 ASCPTLQFTFMSPRTYVTSTQVTSSTKPLVGLRLYLEGKRSNWLALHVQHLTSLPKVMAR 480

Query: 481 LMSSSDVASRCQWRGSDDGDCNGQFLEPVKWKRYSNVCTSAVRHDPNWLQQDGNGVYIVT 540
           LM SSDV+SR +W GSDD   N QFLE VKWK YSNVCTS VRHDPNWLQQDG+GVYIVT
Sbjct: 481 LMGSSDVSSRSRWLGSDDSHSNSQFLEAVKWKSYSNVCTSVVRHDPNWLQQDGDGVYIVT 540

Query: 541 GAQIISKGKWPKTILHLRLQYTHMPNCTIRRTEWAAAPGASRKSTFLTNLSTTFSFTQRP 600
           GAQ+++KGKWPKTILHLRLQ+TH+PNC IRRTEWAAAP   RKSTFLTNLSTTF+FTQR 
Sbjct: 541 GAQLLTKGKWPKTILHLRLQFTHLPNCAIRRTEWAAAPETGRKSTFLTNLSTTFTFTQRA 600

Query: 601 AADKNKQVPGALNSGVYGEAPPVPVRSKKLLKYVDTTEVVRGPHDSPGHWLVTAAKLVTE 659
           A DK KQVPGALNSGVYGEAPPVPVRSKKLLKYVDT EVVRGPHD+PGHWLVTAAKLVTE
Sbjct: 601 AGDKKKQVPGALNSGVYGEAPPVPVRSKKLLKYVDTAEVVRGPHDAPGHWLVTAAKLVTE 660

BLAST of MS000611 vs. NCBI nr
Match: KAG6592885.1 (MACPF domain-containing protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1128.6 bits (2918), Expect = 0.0e+00
Identity = 548/607 (90.28%), Postives = 577/607 (95.06%), Query Frame = 0

Query: 53  MPASEKPVEIRALECLGRGFDLTNDFRLKFVKGYGGGGRRLVVVDDSNRRDMVFPDGAVV 112
           M ++EKPVE RALECLGRGFDLTNDFRLKFVKG G GGRRLVV+DD N+RD+VFPDG VV
Sbjct: 1   MISTEKPVEARALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVV 60

Query: 113 RGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQKSSIQGKVPSGYFNSMFGLSGDWFHDA 172
           +GVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQ+SSIQGKVPSGYFNSMF LSGDWFHDA
Sbjct: 61  QGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFSLSGDWFHDA 120

Query: 173 ADANHLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSSWDPASLSRFIQTYGTHIIVGMA 232
           ADAN+LAFDGYFIALYYLHLTASPLVLQDEVKKAVPS+WDPASLSRFIQTYGTHIIVG A
Sbjct: 121 ADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSNWDPASLSRFIQTYGTHIIVGTA 180

Query: 233 IGGQDLISVRQRPSSPIPPAELRRNLEDLGDVLFSDRRSPSAL-LKRDVKVPEIFTRILQ 292
           IGGQDLISVRQRPSS IPPAELRR+LEDLGD+LFSD+RSPS+L  KRDVKVPEIFT+ILQ
Sbjct: 181 IGGQDLISVRQRPSSSIPPAELRRHLEDLGDILFSDKRSPSSLHRKRDVKVPEIFTKILQ 240

Query: 293 SSSIQLASISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSSKPEVILFKFVPITSLLT 352
           SSSIQLA+ISETSSKDGITLICSKRGGDPFLHSHTSWLQTVS KPEVILFKFVPITSLLT
Sbjct: 241 SSSIQLANISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSFKPEVILFKFVPITSLLT 300

Query: 353 GVAGSGYLSHAINLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQRKKATCPTLQF 412
           GVAGSGYLSHAINLYLRYKPSL DLQYFLEFQIPTQWAPNFCELPLGHQR+KATCPTLQF
Sbjct: 301 GVAGSGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATCPTLQF 360

Query: 413 AFMSPRMYIASTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMASLMSSSDVA 472
            FMSPR YI STQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMA L+ +SD +
Sbjct: 361 TFMSPRTYITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTSDQS 420

Query: 473 SRCQWRGSDDGDCNGQFLEPVKWKRYSNVCTSAVRHDPNWLQQDGNGVYIVTGAQIISKG 532
            R QW G+DD D NG+FLEPVKWKRYSNVCTS VRHDPNWL+QDGNGVYIVTGAQ+ISKG
Sbjct: 421 RRSQWVGTDDSDSNGRFLEPVKWKRYSNVCTSVVRHDPNWLKQDGNGVYIVTGAQLISKG 480

Query: 533 KWPKTILHLRLQYTHMPNCTIRRTEWAAAPGASRKSTFLTNLSTTFSFTQRPAADKNKQV 592
           KWPKTILHLRLQ+TH+PNCTIRRTEWAAAP  SRKSTFLTNLSTTF+FTQR AADK KQV
Sbjct: 481 KWPKTILHLRLQFTHLPNCTIRRTEWAAAPEGSRKSTFLTNLSTTFTFTQRAAADKKKQV 540

Query: 593 PGALNSGVYGEAPPVPVRSKKLLKYVDTTEVVRGPHDSPGHWLVTAAKLVTESGKIGLNV 652
           PGALNSGVYGEAPPVPVRSKKLLKYVDT+EVV+GPHD+PGHWLVTAAKLVTE GKIGLNV
Sbjct: 541 PGALNSGVYGEAPPVPVRSKKLLKYVDTSEVVKGPHDTPGHWLVTAAKLVTEGGKIGLNV 600

Query: 653 KFALLDY 659
           KFALLDY
Sbjct: 601 KFALLDY 607

BLAST of MS000611 vs. NCBI nr
Match: XP_022960284.1 (MACPF domain-containing protein At1g14780-like [Cucurbita moschata])

HSP 1 Score: 1127.9 bits (2916), Expect = 0.0e+00
Identity = 548/607 (90.28%), Postives = 576/607 (94.89%), Query Frame = 0

Query: 53  MPASEKPVEIRALECLGRGFDLTNDFRLKFVKGYGGGGRRLVVVDDSNRRDMVFPDGAVV 112
           M ++EKPVE RALECLGRGFDLTNDFRLKFVKG G GGRRLVV+DD N+RD+VFPDG VV
Sbjct: 1   MISTEKPVEARALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVV 60

Query: 113 RGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQKSSIQGKVPSGYFNSMFGLSGDWFHDA 172
           +GVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQ+SSIQGKVPSGYFNSMF LSGDWFHDA
Sbjct: 61  QGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNLSGDWFHDA 120

Query: 173 ADANHLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSSWDPASLSRFIQTYGTHIIVGMA 232
           ADAN+LAFDGYFIALYYLHLTASPLVLQDEVKKAVPS WDPASLSRFIQTYGTHIIVG A
Sbjct: 121 ADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSHWDPASLSRFIQTYGTHIIVGTA 180

Query: 233 IGGQDLISVRQRPSSPIPPAELRRNLEDLGDVLFSDRRSPSAL-LKRDVKVPEIFTRILQ 292
           IGGQDLISVRQRPSS IPPAELRR+LEDLGD+LFSD+RSPS+L  KRDVKVPEIFT+ILQ
Sbjct: 181 IGGQDLISVRQRPSSSIPPAELRRHLEDLGDILFSDKRSPSSLHRKRDVKVPEIFTKILQ 240

Query: 293 SSSIQLASISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSSKPEVILFKFVPITSLLT 352
           SSSIQLA+ISETSSKDGITLICSKRGGDPFLHSHTSWLQTVS KPEVILFKFVPITSLLT
Sbjct: 241 SSSIQLANISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSFKPEVILFKFVPITSLLT 300

Query: 353 GVAGSGYLSHAINLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQRKKATCPTLQF 412
           GVAGSGYLSHAINLYLRYKPSL DLQYFLEFQIPTQWAPNFCELPLGHQR+KATCPTLQF
Sbjct: 301 GVAGSGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATCPTLQF 360

Query: 413 AFMSPRMYIASTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMASLMSSSDVA 472
            FMSPR YI STQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMA L+ +SD +
Sbjct: 361 TFMSPRTYITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTSDQS 420

Query: 473 SRCQWRGSDDGDCNGQFLEPVKWKRYSNVCTSAVRHDPNWLQQDGNGVYIVTGAQIISKG 532
            R QW G+DD D NG+FLEPVKWKRYSNVCTS VRHDPNWL+QDGNGVYIVTGAQ+ISKG
Sbjct: 421 RRSQWVGTDDSDSNGRFLEPVKWKRYSNVCTSVVRHDPNWLKQDGNGVYIVTGAQLISKG 480

Query: 533 KWPKTILHLRLQYTHMPNCTIRRTEWAAAPGASRKSTFLTNLSTTFSFTQRPAADKNKQV 592
           KWPKTILHLRLQ+TH+PNCTIRRTEWAAAP  SRKSTFLTNLSTTF+FTQR AADK KQV
Sbjct: 481 KWPKTILHLRLQFTHLPNCTIRRTEWAAAPEGSRKSTFLTNLSTTFTFTQRAAADKKKQV 540

Query: 593 PGALNSGVYGEAPPVPVRSKKLLKYVDTTEVVRGPHDSPGHWLVTAAKLVTESGKIGLNV 652
           PGALNSGVYGEAPPVPVRSKKLLKYVDT+EVV+GPHD+PGHWLVTAAKLVTE GKIGLNV
Sbjct: 541 PGALNSGVYGEAPPVPVRSKKLLKYVDTSEVVKGPHDTPGHWLVTAAKLVTEGGKIGLNV 600

Query: 653 KFALLDY 659
           KFALLDY
Sbjct: 601 KFALLDY 607

BLAST of MS000611 vs. ExPASy Swiss-Prot
Match: Q8L612 (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 7.6e-142
Identity = 278/617 (45.06%), Postives = 380/617 (61.59%), Query Frame = 0

Query: 64  ALECLGRGFDLTNDFRLKFVKGYGG--GGRRLVVVDDSNRRDMVFPDGAVVRGVSQDIRC 123
           A++ LG+GFDLT DFRLK+ K   G  G  RLVV+D +  R++  P   V + VS DI C
Sbjct: 12  AVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADINC 71

Query: 124 DKGDRIRFKSDVLQFNQMSELLNQKSSIQGKVPSGYFNSMFGL-SGDWFHDAADANHLAF 183
           DKG+R RF+SD+L FN+MSE  NQ+SS+ GK+PSG FN+ FG  SG W  DAA+   L  
Sbjct: 72  DKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSLGL 131

Query: 184 DGYFIALYYLHL-TASPLVLQDEVKKAVPSSWDPASLSRFIQTYGTHIIVGMAIGGQDLI 243
           D   + L+ LH+   + L L D V+ AVPSSWDP  L+RFI+ YGTH+I G+++GGQD++
Sbjct: 132 DASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQDVV 191

Query: 244 SVRQRPSSPIPPAELRRNLEDLGDVLF------SDRRSPSAL--LKRDVKVPEIFTRILQ 303
            VRQ  SS +    LR +L DLGD LF      S RR   A        K PE F     
Sbjct: 192 VVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAFNVFDD 251

Query: 304 SSSIQLASISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSSKPEVILFKFVPITSLLT 363
             ++   + S  +S++GIT+IC+KRGGD    SH+ WL TV  KP+ I F F+PITSLL 
Sbjct: 252 KQTVAFNNFS-INSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPITSLLK 311

Query: 364 GVAGSGYLSHAINLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQRKKATC-PTLQ 423
            V GSG LSHA++LYLRYKP L DLQYFL+F  P  WAP   +LP G     A+  P L 
Sbjct: 312 DVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASAYPALH 371

Query: 424 FAFMSPRMYIASTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMASLMSSSDV 483
             FM P++Y+ +T VTS + P+ G+R +LEGKK N LA+H+QHL +    +   ++   +
Sbjct: 372 INFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKITDEHI 431

Query: 484 ASRCQWRGSDDGDCNGQFLEPVKWKRYSNVCTSAVRHDPNWLQQDGNG------VYIVTG 543
                WRGSD    N ++ EP+  K++S+VCT  V++DPNW++   N        +IVTG
Sbjct: 432 -----WRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTG 491

Query: 544 AQIISKGKWPKTILHLRLQYTHMPNCTIRRTEWAAAP-GASRKSTFLTNLSTTFS----F 603
           AQ+  K    K++LHLRL+YT + +  + +  W   P G S+KS   +++S   +     
Sbjct: 492 AQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSVH 551

Query: 604 TQRPAADKNKQVPGALNSGVYGEAPPVPVRSKKLLKYVDTTEVVRGPHDSPGHWLVTAAK 657
                 DKN+ V   L+SGV+   PPVP  + K++K+VD +++ RGP  SPGHWLVT  +
Sbjct: 552 HNMIQKDKNEVV---LDSGVFPGGPPVPA-NNKIVKFVDLSQLCRGPQHSPGHWLVTGVR 611

BLAST of MS000611 vs. ExPASy Swiss-Prot
Match: Q9STW5 (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 495.7 bits (1275), Expect = 7.9e-139
Identity = 266/613 (43.39%), Postives = 390/613 (63.62%), Query Frame = 0

Query: 53  MPASEKPVEIRALECLGRGFDLTNDFRLKFVKGYGGGGRRLVVVDDSNRRDMVFPDGAVV 112
           +PAS K  E+ A+  +G G+DL  D RLK+ KG     R L + +  +  ++V P G  +
Sbjct: 5   LPAS-KAAEV-AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISI 64

Query: 113 RGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQKSSIQGKVPSGYFNSMFGLSGDWFHDA 172
             VS+ I+CDKG+R+RF+SD+L F QM+E  NQ+ S+ GK+PSG FN+MF  S  W  DA
Sbjct: 65  PNVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDA 124

Query: 173 ADANHLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSSWDPASLSRFIQTYGTHIIVGMA 232
           A   +LAFDG FI+LY + L  S ++L++ VK+AVPS+WDPA+L+RFI  YGTHIIV + 
Sbjct: 125 AYTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVK 184

Query: 233 IGGQDLISVRQRPSSPIPPAELRRNLEDLGDVLFSD----RRSPSALLKRDVKVPEIFTR 292
           +GG+D+I  +Q+ SS + P +L++ L+++ D  F +      + S  ++   KV    T+
Sbjct: 185 MGGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVE---TK 244

Query: 293 ILQSSSIQLASISETSSKDGITLICSKRGGDPFLH-SHTSWLQTVSSKPEVILFKFVPIT 352
             +      +S+   ++K+    +C +RGG+   +  H  WLQTV  +P+VI   F+PIT
Sbjct: 245 EQRLRFADTSSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPIT 304

Query: 353 SLLTGVAGSGYLSHAINLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQRKKATCP 412
           SLL GV GSG+LSHAINLYLRYKP + +L  FLEFQ+P QWAP F ELPLG QRK+ +C 
Sbjct: 305 SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCA 364

Query: 413 TLQFAFMSPRMYIASTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMASLMSS 472
           +LQF+F  P++Y+ +T V   ++P+ G+RLYLEG++SN LA+H+QHL+SLPK+       
Sbjct: 365 SLQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKI----YQL 424

Query: 473 SDVASRCQWRGSDDGDCNGQFLEPVKWKRYSNVCTSAVRHDPNWLQQDGNGVYIVTGAQI 532
            D  +R   + S D     ++ E V WK YS+VCT  V  D        + + +VTGAQ+
Sbjct: 425 EDDLNRSIRQESHD----RRYYEKVNWKNYSHVCTEPVESD--------DDLSVVTGAQL 484

Query: 533 ISKGKWPKTILHLRLQYTHMPNCT-IRRTEWAAAPGASRKSTFLTNL-STTFSFTQRP-- 592
             +    K +L LRL ++ +   T ++ +EW  A G + KS  ++ L S  F+  Q+P  
Sbjct: 485 HVESHGFKNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPP 544

Query: 593 -AADKNKQVPGALNSGVYGEAPPVPVRSKKLLKYVDTTEVVRGPHDSPGHWLVTAAKLVT 652
             AD N      +NS +Y   PPVP ++ KLLK+VDT+E+ RGP +SPG+W+V+ A+L+ 
Sbjct: 545 RPADVN------INSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLV 590

Query: 653 ESGKIGLNVKFAL 656
           E GKI L VK++L
Sbjct: 605 EKGKISLKVKYSL 590

BLAST of MS000611 vs. ExPASy Swiss-Prot
Match: Q9SGN6 (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=2 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 7.4e-129
Identity = 257/602 (42.69%), Postives = 365/602 (60.63%), Query Frame = 0

Query: 63  RALECLGRGFDLTNDFRLKFVKGYGGGGRRLVVVDDSNRRDMVFPDGAVVRGVSQDIRCD 122
           +A+  +G G+DL +D R    K    G  RLV +D +  RD++FP G VV  VS  I+CD
Sbjct: 17  KAVSVIGLGYDLCSDVRFSACKTTPDGS-RLVEIDPTRNRDLIFPGGIVVNNVSSSIKCD 76

Query: 123 KGDRIRFKSDVLQFNQMSELLNQKSSIQGKVPSGYFNSMFGLSGDWFHDAADANHLAFDG 182
           KG+R R +SD+L FNQMSE  NQ   + GK+PSG FN+MF  S  W  DA+    LA+DG
Sbjct: 77  KGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLAYDG 136

Query: 183 YFIALYYLHLTASPLVLQDEVKKAVPSSWDPASLSRFIQTYGTHIIVGMAIGGQDLISVR 242
           +FI+LY + +    L L+DEVK+ VPSSWD A+L+ FI+ YGTH++VG+ +GG+D+I V+
Sbjct: 137 WFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIHVK 196

Query: 243 QRPSSPIPPAELRRNLEDLGDVLFS----DRRSPSALLKRDVKVPEIFTRILQ--SSSIQ 302
           Q   S   P E+++ L+  GD  F     + +SP+++     K   +    LQ   +S+ 
Sbjct: 197 QMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQWGLQPFGTSVS 256

Query: 303 LASISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSSKPEVILFKFVPITSLLTGVAGS 362
            A +  T +++ I  +C +RGG     SH  WL TVS  P VI   FVPITSLL+G+ G+
Sbjct: 257 SAVVMHTKNEE-IMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLSGLPGT 316

Query: 363 GYLSHAINLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQRKKATCPTLQFAFMSP 422
           G+LSHA+NLYLRYKP + +L  FLEFQ+P QWAP + +LPLG +R K + P+LQF+ M P
Sbjct: 317 GFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQFSLMGP 376

Query: 423 RMYIASTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMASLMSSSDVASRCQW 482
           ++Y+ +++V S  +P+ GLR +LEGKK N LA+H+QHL++ P    SL  S D       
Sbjct: 377 KLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACP---PSLHLSHDDTYEPIE 436

Query: 483 RGSDDGDCNGQFLEPVKWKRYSNVCTSAVRHDPNWLQQDGNGVYIVTGAQIISKGKWPKT 542
              + G     +  PVKW  +S+VCT  V++  N  + D +   IVT A +  KG   + 
Sbjct: 437 EPVEKG-----YYVPVKWGIFSHVCTYPVQY--NGARSD-DTASIVTKAWLEVKGMGMRK 496

Query: 543 ILHLRLQYTHMPNCTIRRTEWAAAPGASRKSTFLTNLSTTFS--FTQRPAADKNKQVPGA 602
           +L LRL ++   +   R++ W      SRKS   + +ST  S   +  PA  K  Q    
Sbjct: 497 VLFLRLGFSLDASAVTRKSCWDNLSTNSRKSGVFSMISTRLSTGLSPNPATTK-PQSKID 556

Query: 603 LNSGVYGEAPPVPVRSKKLLKYVDTTEVVRGPHDSPGHWLVTAAKLVTESGKIGLNVKFA 657
           +NS VY   P  PV+  KLL  VDT EV+RGP + PG+W+VT AKL  E+GKI +  K++
Sbjct: 557 INSAVYPRGPSPPVK-PKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYS 603

BLAST of MS000611 vs. ExPASy Swiss-Prot
Match: Q9C7N2 (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=2 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 3.9e-122
Identity = 241/598 (40.30%), Postives = 343/598 (57.36%), Query Frame = 0

Query: 64  ALECLGRGFDLTNDFRLKFVKGYGGGGRRLVVVDDSNRRDMVFPDGAVVRGVSQDIRCDK 123
           A++ LGRGFD+T+D RL + K  G  G RLV +++   RD+    G ++  V  DI C +
Sbjct: 24  AIQALGRGFDVTSDVRLLYCK--GAPGSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSR 83

Query: 124 GDRIRFKSDVLQFNQMSELLNQKSSIQGKVPSGYFNSMFGLSGDWFHDAADANHLAFDGY 183
           G+    +  V  F++M+E  N +S ++G +P G FN+MF  +G W  DAA    LA  GY
Sbjct: 84  GNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGY 143

Query: 184 FIALYYLHLTASPLVLQDEVKKAVPSSWDPASLSRFIQTYGTHIIVGMAIGGQDLISVRQ 243
           FI LY + L    LVL +E+++AVPSSWDPASL+ FI+ YGTHI+  + IGG+D++ +RQ
Sbjct: 144 FIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQ 203

Query: 244 RPSSPIPPAELRRNLEDLGDVLFSDRRSPSALLKRDVKVPEIFTRILQSSSIQLASISET 303
             SSP+P +E+   + D+                       I  R  ++ S  +    + 
Sbjct: 204 HQSSPLPVSEIENYVNDM-----------------------IKHRFHEAESQSITGPLKY 263

Query: 304 SSKDGITLICSKRGGDPFLHSHTSWLQTVSSKPEVILFKFVPITSLLTGVAGSGYLSHAI 363
             KD IT+I  +RGGD    SH  W +TV + P++I   F PI SLL GV G  +L+ AI
Sbjct: 264 KDKD-ITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHLTRAI 323

Query: 364 NLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQRKKATCPTLQFAFMSPRMYIAST 423
            LYL YKP + DLQYFL++QI   WAP    L    QRK+  C +LQF+ M P+++I++ 
Sbjct: 324 ELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGPKLFISAD 383

Query: 424 QVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMASLMSSSDVASRCQWRGSDDGD 483
           QVT  RKP+ GLRL LEG K N L++H+QHL SLPK++     S       +W+G ++ D
Sbjct: 384 QVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWDSHVPIGAPKWQGPEEQD 443

Query: 484 CNGQFLEPVKWKRYSNVCTSAVRHDPNWLQQDGNGVYIVTGAQIISKGKW---PKTILHL 543
              ++ EP+KWK +S+V TS + H    +  D +GV+IVTGAQ+   G W    K +LHL
Sbjct: 444 --SRWFEPIKWKNFSHVSTSPIEHTETHI-GDLSGVHIVTGAQL---GVWDFGSKNVLHL 503

Query: 544 RLQYTHMPNCTIRRTEWAAAPGASRKSTFLTNLSTTFSFTQRPAADKNKQVPGALNSGVY 603
           +L ++ +P CTIRR+ W                         P A   +  PG  ++   
Sbjct: 504 KLLFSKVPGCTIRRSVW----------------------DHTPVASSGRLEPGGPSTS-- 561

Query: 604 GEAPPVPVRSKKLLKYVDTTEVVRGPHDSPGHWLVTAAKLVTESGKIGLNVKFALLDY 659
                V  +S KL K VD++E+++GP D PGHWLVT AKL  E GKI L VK++LL+Y
Sbjct: 564 SSTEEVSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 561

BLAST of MS000611 vs. ExPASy TrEMBL
Match: A0A6J1D8F9 (MACPF domain-containing protein At1g14780-like OS=Momordica charantia OX=3673 GN=LOC111018588 PE=4 SV=1)

HSP 1 Score: 1236.5 bits (3198), Expect = 0.0e+00
Identity = 605/606 (99.83%), Postives = 605/606 (99.83%), Query Frame = 0

Query: 53  MPASEKPVEIRALECLGRGFDLTNDFRLKFVKGYGGGGRRLVVVDDSNRRDMVFPDGAVV 112
           MPASEKPVEIRALECLGRGFDLTNDFRLKFVKGYGGGGRRLVVVDDSNRRDMVFPDGAVV
Sbjct: 1   MPASEKPVEIRALECLGRGFDLTNDFRLKFVKGYGGGGRRLVVVDDSNRRDMVFPDGAVV 60

Query: 113 RGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQKSSIQGKVPSGYFNSMFGLSGDWFHDA 172
           RGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQKSSIQGKVPSGYFNSMFGLSGDWFHDA
Sbjct: 61  RGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQKSSIQGKVPSGYFNSMFGLSGDWFHDA 120

Query: 173 ADANHLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSSWDPASLSRFIQTYGTHIIVGMA 232
           ADANHLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSSWDPASLSRFIQTYGTHIIVGMA
Sbjct: 121 ADANHLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSSWDPASLSRFIQTYGTHIIVGMA 180

Query: 233 IGGQDLISVRQRPSSPIPPAELRRNLEDLGDVLFSDRRSPSALLKRDVKVPEIFTRILQS 292
           IGGQDLISVRQRPSSPIPPAELRRNLEDLGDVLFSDRRSPSALLKRDVKVPEIFTRILQS
Sbjct: 181 IGGQDLISVRQRPSSPIPPAELRRNLEDLGDVLFSDRRSPSALLKRDVKVPEIFTRILQS 240

Query: 293 SSIQLASISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSSKPEVILFKFVPITSLLTG 352
           SSIQLASISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSSKPEVILFKFVPITSLLTG
Sbjct: 241 SSIQLASISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSSKPEVILFKFVPITSLLTG 300

Query: 353 VAGSGYLSHAINLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQRKKATCPTLQFA 412
           VAGSGYLSHAINLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQRKKATCPTLQFA
Sbjct: 301 VAGSGYLSHAINLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQRKKATCPTLQFA 360

Query: 413 FMSPRMYIASTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMASLMSSSDVAS 472
           FMSPRMYIASTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLP VMASLMSSSDVAS
Sbjct: 361 FMSPRMYIASTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPTVMASLMSSSDVAS 420

Query: 473 RCQWRGSDDGDCNGQFLEPVKWKRYSNVCTSAVRHDPNWLQQDGNGVYIVTGAQIISKGK 532
           RCQWRGSDDGDCNGQFLEPVKWKRYSNVCTSAVRHDPNWLQQDGNGVYIVTGAQIISKGK
Sbjct: 421 RCQWRGSDDGDCNGQFLEPVKWKRYSNVCTSAVRHDPNWLQQDGNGVYIVTGAQIISKGK 480

Query: 533 WPKTILHLRLQYTHMPNCTIRRTEWAAAPGASRKSTFLTNLSTTFSFTQRPAADKNKQVP 592
           WPKTILHLRLQYTHMPNCTIRRTEWAAAPGASRKSTFLTNLSTTFSFTQRPAADKNKQVP
Sbjct: 481 WPKTILHLRLQYTHMPNCTIRRTEWAAAPGASRKSTFLTNLSTTFSFTQRPAADKNKQVP 540

Query: 593 GALNSGVYGEAPPVPVRSKKLLKYVDTTEVVRGPHDSPGHWLVTAAKLVTESGKIGLNVK 652
           GALNSGVYGEAPPVPVRSKKLLKYVDTTEVVRGPHDSPGHWLVTAAKLVTESGKIGLNVK
Sbjct: 541 GALNSGVYGEAPPVPVRSKKLLKYVDTTEVVRGPHDSPGHWLVTAAKLVTESGKIGLNVK 600

Query: 653 FALLDY 659
           FALLDY
Sbjct: 601 FALLDY 606

BLAST of MS000611 vs. ExPASy TrEMBL
Match: A0A0A0KDR9 (MACPF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G022370 PE=4 SV=1)

HSP 1 Score: 1160.6 bits (3001), Expect = 0.0e+00
Identity = 584/675 (86.52%), Postives = 610/675 (90.37%), Query Frame = 0

Query: 1   MNRILSGQIWGQVKFSDLYLLLLLHLL-----LTHLNLIVLFVWLLRFLV---------- 60
           M RILSGQIWGQVKFSDLYLLLLL  L     LT LNLI  F W L F V          
Sbjct: 1   MIRILSGQIWGQVKFSDLYLLLLLLFLLLLPPLTFLNLIP-FAWFLPFFVVDHQLTSSFD 60

Query: 61  DL-ISSSAMPASEKPVEIRALECLGRGFDLTNDFRLKFVKGYGGGGRRLVVVDDSNRRDM 120
           DL +  SAM + EK VE+RAL+CLGRGFDLTNDFRLKFVKG GGGGRRLVVVDD N+RDM
Sbjct: 61  DLAVGGSAMLSPEKSVEVRALDCLGRGFDLTNDFRLKFVKGNGGGGRRLVVVDDVNKRDM 120

Query: 121 VFPDGAVVRGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQKSSIQGKVPSGYFNSMFGL 180
           VFPDGAVVRGVSQDIRCDKGDRIRFKSDVLQFNQMSE LNQKSSIQGKVPSGYFNSMF L
Sbjct: 121 VFPDGAVVRGVSQDIRCDKGDRIRFKSDVLQFNQMSETLNQKSSIQGKVPSGYFNSMFDL 180

Query: 181 SGDWFHDAADANHLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSSWDPASLSRFIQTYG 240
           SGDWFHDAADAN+LAFDGYFIALYYLHLTASPLVLQDEVKKAVPS+WDPASL+RFIQTYG
Sbjct: 181 SGDWFHDAADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSNWDPASLARFIQTYG 240

Query: 241 THIIVGMAIGGQDLISVRQRPSSPIPPAELRRNLEDLGDVLFSDRRSPSAL-LKRDVKVP 300
           THIIVG AIGGQDLISVRQRPSSPIPPAE+RR+LEDLGD+LFSD+RSPS L  +RD KVP
Sbjct: 241 THIIVGTAIGGQDLISVRQRPSSPIPPAEVRRHLEDLGDILFSDKRSPSTLQRRRDGKVP 300

Query: 301 EIFTRILQSSSIQLASISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSSKPEVILFKF 360
           EIFTRILQSSSIQL SISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSSKPEVILFKF
Sbjct: 301 EIFTRILQSSSIQLTSISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSSKPEVILFKF 360

Query: 361 VPITSLLTGVAGSGYLSHAINLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQRKK 420
           VPITSLLTGVAGSGYLSHAINLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQRKK
Sbjct: 361 VPITSLLTGVAGSGYLSHAINLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQRKK 420

Query: 421 ATCPTLQFAFMSPRMYIASTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMAS 480
           A+CPTLQF FMSPR Y+ STQVTSS KPLVGLRLYLEGK+SNWLALHVQHLTSLPKVMA 
Sbjct: 421 ASCPTLQFTFMSPRTYVTSTQVTSSTKPLVGLRLYLEGKRSNWLALHVQHLTSLPKVMAR 480

Query: 481 LMSSSDVASRCQWRGSDDGDCNGQFLEPVKWKRYSNVCTSAVRHDPNWLQQDGNGVYIVT 540
           LM SSDV+SR +W GSDD   N QFLE VKWK YSNVCTS VRHDPNWLQQDG+GVYIVT
Sbjct: 481 LMGSSDVSSRSRWLGSDDSHSNSQFLEAVKWKSYSNVCTSVVRHDPNWLQQDGDGVYIVT 540

Query: 541 GAQIISKGKWPKTILHLRLQYTHMPNCTIRRTEWAAAPGASRKSTFLTNLSTTFSFTQRP 600
           GAQ+++KGKWPKTILHLRLQ+TH+PNC IRRTEWAAAP   RKSTFLTNLSTTF+FTQR 
Sbjct: 541 GAQLLTKGKWPKTILHLRLQFTHLPNCAIRRTEWAAAPETGRKSTFLTNLSTTFTFTQRA 600

Query: 601 AADKNKQVPGALNSGVYGEAPPVPVRSKKLLKYVDTTEVVRGPHDSPGHWLVTAAKLVTE 659
           A DK KQVPGALNSGVYGEAPPVPVRSKKLLKYVDT EVVRGPHD+PGHWLVTAAKLVTE
Sbjct: 601 AGDKKKQVPGALNSGVYGEAPPVPVRSKKLLKYVDTAEVVRGPHDAPGHWLVTAAKLVTE 660

BLAST of MS000611 vs. ExPASy TrEMBL
Match: A0A6J1HAI4 (MACPF domain-containing protein At1g14780-like OS=Cucurbita moschata OX=3662 GN=LOC111461072 PE=4 SV=1)

HSP 1 Score: 1127.9 bits (2916), Expect = 0.0e+00
Identity = 548/607 (90.28%), Postives = 576/607 (94.89%), Query Frame = 0

Query: 53  MPASEKPVEIRALECLGRGFDLTNDFRLKFVKGYGGGGRRLVVVDDSNRRDMVFPDGAVV 112
           M ++EKPVE RALECLGRGFDLTNDFRLKFVKG G GGRRLVV+DD N+RD+VFPDG VV
Sbjct: 1   MISTEKPVEARALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVV 60

Query: 113 RGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQKSSIQGKVPSGYFNSMFGLSGDWFHDA 172
           +GVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQ+SSIQGKVPSGYFNSMF LSGDWFHDA
Sbjct: 61  QGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNLSGDWFHDA 120

Query: 173 ADANHLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSSWDPASLSRFIQTYGTHIIVGMA 232
           ADAN+LAFDGYFIALYYLHLTASPLVLQDEVKKAVPS WDPASLSRFIQTYGTHIIVG A
Sbjct: 121 ADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSHWDPASLSRFIQTYGTHIIVGTA 180

Query: 233 IGGQDLISVRQRPSSPIPPAELRRNLEDLGDVLFSDRRSPSAL-LKRDVKVPEIFTRILQ 292
           IGGQDLISVRQRPSS IPPAELRR+LEDLGD+LFSD+RSPS+L  KRDVKVPEIFT+ILQ
Sbjct: 181 IGGQDLISVRQRPSSSIPPAELRRHLEDLGDILFSDKRSPSSLHRKRDVKVPEIFTKILQ 240

Query: 293 SSSIQLASISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSSKPEVILFKFVPITSLLT 352
           SSSIQLA+ISETSSKDGITLICSKRGGDPFLHSHTSWLQTVS KPEVILFKFVPITSLLT
Sbjct: 241 SSSIQLANISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSFKPEVILFKFVPITSLLT 300

Query: 353 GVAGSGYLSHAINLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQRKKATCPTLQF 412
           GVAGSGYLSHAINLYLRYKPSL DLQYFLEFQIPTQWAPNFCELPLGHQR+KATCPTLQF
Sbjct: 301 GVAGSGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATCPTLQF 360

Query: 413 AFMSPRMYIASTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMASLMSSSDVA 472
            FMSPR YI STQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMA L+ +SD +
Sbjct: 361 TFMSPRTYITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTSDQS 420

Query: 473 SRCQWRGSDDGDCNGQFLEPVKWKRYSNVCTSAVRHDPNWLQQDGNGVYIVTGAQIISKG 532
            R QW G+DD D NG+FLEPVKWKRYSNVCTS VRHDPNWL+QDGNGVYIVTGAQ+ISKG
Sbjct: 421 RRSQWVGTDDSDSNGRFLEPVKWKRYSNVCTSVVRHDPNWLKQDGNGVYIVTGAQLISKG 480

Query: 533 KWPKTILHLRLQYTHMPNCTIRRTEWAAAPGASRKSTFLTNLSTTFSFTQRPAADKNKQV 592
           KWPKTILHLRLQ+TH+PNCTIRRTEWAAAP  SRKSTFLTNLSTTF+FTQR AADK KQV
Sbjct: 481 KWPKTILHLRLQFTHLPNCTIRRTEWAAAPEGSRKSTFLTNLSTTFTFTQRAAADKKKQV 540

Query: 593 PGALNSGVYGEAPPVPVRSKKLLKYVDTTEVVRGPHDSPGHWLVTAAKLVTESGKIGLNV 652
           PGALNSGVYGEAPPVPVRSKKLLKYVDT+EVV+GPHD+PGHWLVTAAKLVTE GKIGLNV
Sbjct: 541 PGALNSGVYGEAPPVPVRSKKLLKYVDTSEVVKGPHDTPGHWLVTAAKLVTEGGKIGLNV 600

Query: 653 KFALLDY 659
           KFALLDY
Sbjct: 601 KFALLDY 607

BLAST of MS000611 vs. ExPASy TrEMBL
Match: A0A6J1KTM0 (MACPF domain-containing protein At1g14780-like OS=Cucurbita maxima OX=3661 GN=LOC111498109 PE=4 SV=1)

HSP 1 Score: 1121.7 bits (2900), Expect = 0.0e+00
Identity = 546/607 (89.95%), Postives = 575/607 (94.73%), Query Frame = 0

Query: 53  MPASEKPVEIRALECLGRGFDLTNDFRLKFVKGYGGGGRRLVVVDDSNRRDMVFPDGAVV 112
           M ++EKPVE RALECLGRGFDLTNDFRLKFVKG G GGRRLVV+DD N+RD+VFPDG VV
Sbjct: 1   MISTEKPVEARALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVV 60

Query: 113 RGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQKSSIQGKVPSGYFNSMFGLSGDWFHDA 172
           +GVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQ+SSIQGKVPSGYFNSMF LSGDWFHDA
Sbjct: 61  QGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNLSGDWFHDA 120

Query: 173 ADANHLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSSWDPASLSRFIQTYGTHIIVGMA 232
           ADAN+LAFDGYFIAL YLHLTASPLVLQDEVKKAVPS+WDPASLSRFIQTYGTHIIVG A
Sbjct: 121 ADANYLAFDGYFIALCYLHLTASPLVLQDEVKKAVPSNWDPASLSRFIQTYGTHIIVGTA 180

Query: 233 IGGQDLISVRQRPSSPIPPAELRRNLEDLGDVLFSDRRSPSAL-LKRDVKVPEIFTRILQ 292
           IGGQDLISVRQRPSS IPPAELRR+LEDLGD+LFSD+RSPS+L  KRDVKVPEIFT+ILQ
Sbjct: 181 IGGQDLISVRQRPSSSIPPAELRRHLEDLGDILFSDKRSPSSLHRKRDVKVPEIFTKILQ 240

Query: 293 SSSIQLASISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSSKPEVILFKFVPITSLLT 352
           SSSIQLA+ISETSSKDGITLICSKRGGDPFLHSHTSWLQTVS KPEVILFKFVPITSLLT
Sbjct: 241 SSSIQLANISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSFKPEVILFKFVPITSLLT 300

Query: 353 GVAGSGYLSHAINLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQRKKATCPTLQF 412
           GVAGSGYLSHAINLYLRYKPSL DLQYFLEFQIPTQWAPNFCELPLGHQR+KATCPTLQF
Sbjct: 301 GVAGSGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATCPTLQF 360

Query: 413 AFMSPRMYIASTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMASLMSSSDVA 472
            FMSPR YI STQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMA L+ +SD +
Sbjct: 361 TFMSPRTYITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTSDES 420

Query: 473 SRCQWRGSDDGDCNGQFLEPVKWKRYSNVCTSAVRHDPNWLQQDGNGVYIVTGAQIISKG 532
            R QW G+DD D NG+FLEPVKWKRYSNVCTS VRHDPNWL+QD NGVYIVTGAQ+ISKG
Sbjct: 421 RRSQWIGTDDSDSNGRFLEPVKWKRYSNVCTSVVRHDPNWLKQDVNGVYIVTGAQLISKG 480

Query: 533 KWPKTILHLRLQYTHMPNCTIRRTEWAAAPGASRKSTFLTNLSTTFSFTQRPAADKNKQV 592
           KWPKTILHLRLQ+TH+PNCTIRRTEWAAAP  SRKSTFLTNLSTTF+FTQR AADK KQV
Sbjct: 481 KWPKTILHLRLQFTHLPNCTIRRTEWAAAPEGSRKSTFLTNLSTTFTFTQRAAADKKKQV 540

Query: 593 PGALNSGVYGEAPPVPVRSKKLLKYVDTTEVVRGPHDSPGHWLVTAAKLVTESGKIGLNV 652
           PGALNSGVYGEAPPVPVRSKKLLKYVDT+EVV+GPHD+PGHWLVTAAKLVTE GKIGLNV
Sbjct: 541 PGALNSGVYGEAPPVPVRSKKLLKYVDTSEVVKGPHDTPGHWLVTAAKLVTEGGKIGLNV 600

Query: 653 KFALLDY 659
           KFALLDY
Sbjct: 601 KFALLDY 607

BLAST of MS000611 vs. ExPASy TrEMBL
Match: A0A5D3BTB4 (MACPF domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold69G00060 PE=4 SV=1)

HSP 1 Score: 1119.0 bits (2893), Expect = 0.0e+00
Identity = 546/607 (89.95%), Postives = 569/607 (93.74%), Query Frame = 0

Query: 53  MPASEKPVEIRALECLGRGFDLTNDFRLKFVKGYGGGGRRLVVVDDSNRRDMVFPDGAVV 112
           M + EK VE+RAL+CLGRGFDLTNDFRLKFVKG G GGRRLVVVDD N+RDMVFPDGAVV
Sbjct: 1   MLSPEKSVEVRALDCLGRGFDLTNDFRLKFVKGNGAGGRRLVVVDDVNKRDMVFPDGAVV 60

Query: 113 RGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQKSSIQGKVPSGYFNSMFGLSGDWFHDA 172
           RGVSQDIRCDKGDRIRFKSDVLQFNQMSE LNQKSSIQGKVPSGYFNSMF LSGDWFHDA
Sbjct: 61  RGVSQDIRCDKGDRIRFKSDVLQFNQMSETLNQKSSIQGKVPSGYFNSMFDLSGDWFHDA 120

Query: 173 ADANHLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSSWDPASLSRFIQTYGTHIIVGMA 232
           ADAN+LAFDGYFIALYYLHLTASPLVLQDEVKKAVPS+WDPASL+RFIQTYGTHIIVGMA
Sbjct: 121 ADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSNWDPASLARFIQTYGTHIIVGMA 180

Query: 233 IGGQDLISVRQRPSSPIPPAELRRNLEDLGDVLFSDRRSPSAL-LKRDVKVPEIFTRILQ 292
           IGGQDLISVRQRPSSPIPPAELRR+LEDLGD+LFSD+RSPS L  KRD KVPEIFTRILQ
Sbjct: 181 IGGQDLISVRQRPSSPIPPAELRRHLEDLGDILFSDKRSPSTLQRKRDGKVPEIFTRILQ 240

Query: 293 SSSIQLASISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSSKPEVILFKFVPITSLLT 352
           SSSIQL SISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSSKPEVILFKFVPITSLLT
Sbjct: 241 SSSIQLTSISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSSKPEVILFKFVPITSLLT 300

Query: 353 GVAGSGYLSHAINLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQRKKATCPTLQF 412
           GVAGSGYLSHAINLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQRKKA+CPTLQF
Sbjct: 301 GVAGSGYLSHAINLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQRKKASCPTLQF 360

Query: 413 AFMSPRMYIASTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMASLMSSSDVA 472
            FMSPR Y+ STQVTSS KPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMA LM +SDV 
Sbjct: 361 TFMSPRTYVTSTQVTSSTKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLMGTSDVL 420

Query: 473 SRCQWRGSDDGDCNGQFLEPVKWKRYSNVCTSAVRHDPNWLQQDGNGVYIVTGAQIISKG 532
           SR +W GSDD D + QFLE VKWK YSNVCTS VRHD NWLQQDG+GVYIVTGAQ+I+KG
Sbjct: 421 SRSRWLGSDDSDSSSQFLEAVKWKSYSNVCTSVVRHDSNWLQQDGDGVYIVTGAQLITKG 480

Query: 533 KWPKTILHLRLQYTHMPNCTIRRTEWAAAPGASRKSTFLTNLSTTFSFTQRPAADKNKQV 592
           KWPKTILHLRLQ++H+PNC IRRTEWAAAP   RKSTFLTNLSTTF+FTQR A DK KQV
Sbjct: 481 KWPKTILHLRLQFSHLPNCAIRRTEWAAAPETGRKSTFLTNLSTTFTFTQRAAGDKKKQV 540

Query: 593 PGALNSGVYGEAPPVPVRSKKLLKYVDTTEVVRGPHDSPGHWLVTAAKLVTESGKIGLNV 652
           PGALNSGVYGEAPPVPVRSKKLLKYVDT EVVRGPHD+PGHWLVTAAKLVTE GKIGLNV
Sbjct: 541 PGALNSGVYGEAPPVPVRSKKLLKYVDTAEVVRGPHDAPGHWLVTAAKLVTEGGKIGLNV 600

Query: 653 KFALLDY 659
           KFALLDY
Sbjct: 601 KFALLDY 607

BLAST of MS000611 vs. TAIR 10
Match: AT1G14780.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 505.8 bits (1301), Expect = 5.4e-143
Identity = 278/617 (45.06%), Postives = 380/617 (61.59%), Query Frame = 0

Query: 64  ALECLGRGFDLTNDFRLKFVKGYGG--GGRRLVVVDDSNRRDMVFPDGAVVRGVSQDIRC 123
           A++ LG+GFDLT DFRLK+ K   G  G  RLVV+D +  R++  P   V + VS DI C
Sbjct: 12  AVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADINC 71

Query: 124 DKGDRIRFKSDVLQFNQMSELLNQKSSIQGKVPSGYFNSMFGL-SGDWFHDAADANHLAF 183
           DKG+R RF+SD+L FN+MSE  NQ+SS+ GK+PSG FN+ FG  SG W  DAA+   L  
Sbjct: 72  DKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSLGL 131

Query: 184 DGYFIALYYLHL-TASPLVLQDEVKKAVPSSWDPASLSRFIQTYGTHIIVGMAIGGQDLI 243
           D   + L+ LH+   + L L D V+ AVPSSWDP  L+RFI+ YGTH+I G+++GGQD++
Sbjct: 132 DASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQDVV 191

Query: 244 SVRQRPSSPIPPAELRRNLEDLGDVLF------SDRRSPSAL--LKRDVKVPEIFTRILQ 303
            VRQ  SS +    LR +L DLGD LF      S RR   A        K PE F     
Sbjct: 192 VVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAFNVFDD 251

Query: 304 SSSIQLASISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSSKPEVILFKFVPITSLLT 363
             ++   + S  +S++GIT+IC+KRGGD    SH+ WL TV  KP+ I F F+PITSLL 
Sbjct: 252 KQTVAFNNFS-INSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPITSLLK 311

Query: 364 GVAGSGYLSHAINLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQRKKATC-PTLQ 423
            V GSG LSHA++LYLRYKP L DLQYFL+F  P  WAP   +LP G     A+  P L 
Sbjct: 312 DVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASAYPALH 371

Query: 424 FAFMSPRMYIASTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMASLMSSSDV 483
             FM P++Y+ +T VTS + P+ G+R +LEGKK N LA+H+QHL +    +   ++   +
Sbjct: 372 INFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKITDEHI 431

Query: 484 ASRCQWRGSDDGDCNGQFLEPVKWKRYSNVCTSAVRHDPNWLQQDGNG------VYIVTG 543
                WRGSD    N ++ EP+  K++S+VCT  V++DPNW++   N        +IVTG
Sbjct: 432 -----WRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTG 491

Query: 544 AQIISKGKWPKTILHLRLQYTHMPNCTIRRTEWAAAP-GASRKSTFLTNLSTTFS----F 603
           AQ+  K    K++LHLRL+YT + +  + +  W   P G S+KS   +++S   +     
Sbjct: 492 AQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSVH 551

Query: 604 TQRPAADKNKQVPGALNSGVYGEAPPVPVRSKKLLKYVDTTEVVRGPHDSPGHWLVTAAK 657
                 DKN+ V   L+SGV+   PPVP  + K++K+VD +++ RGP  SPGHWLVT  +
Sbjct: 552 HNMIQKDKNEVV---LDSGVFPGGPPVPA-NNKIVKFVDLSQLCRGPQHSPGHWLVTGVR 611

BLAST of MS000611 vs. TAIR 10
Match: AT4G24290.2 (MAC/Perforin domain-containing protein )

HSP 1 Score: 495.7 bits (1275), Expect = 5.6e-140
Identity = 266/613 (43.39%), Postives = 390/613 (63.62%), Query Frame = 0

Query: 53  MPASEKPVEIRALECLGRGFDLTNDFRLKFVKGYGGGGRRLVVVDDSNRRDMVFPDGAVV 112
           +PAS K  E+ A+  +G G+DL  D RLK+ KG     R L + +  +  ++V P G  +
Sbjct: 5   LPAS-KAAEV-AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISI 64

Query: 113 RGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQKSSIQGKVPSGYFNSMFGLSGDWFHDA 172
             VS+ I+CDKG+R+RF+SD+L F QM+E  NQ+ S+ GK+PSG FN+MF  S  W  DA
Sbjct: 65  PNVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDA 124

Query: 173 ADANHLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSSWDPASLSRFIQTYGTHIIVGMA 232
           A   +LAFDG FI+LY + L  S ++L++ VK+AVPS+WDPA+L+RFI  YGTHIIV + 
Sbjct: 125 AYTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVK 184

Query: 233 IGGQDLISVRQRPSSPIPPAELRRNLEDLGDVLFSD----RRSPSALLKRDVKVPEIFTR 292
           +GG+D+I  +Q+ SS + P +L++ L+++ D  F +      + S  ++   KV    T+
Sbjct: 185 MGGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVE---TK 244

Query: 293 ILQSSSIQLASISETSSKDGITLICSKRGGDPFLH-SHTSWLQTVSSKPEVILFKFVPIT 352
             +      +S+   ++K+    +C +RGG+   +  H  WLQTV  +P+VI   F+PIT
Sbjct: 245 EQRLRFADTSSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPIT 304

Query: 353 SLLTGVAGSGYLSHAINLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQRKKATCP 412
           SLL GV GSG+LSHAINLYLRYKP + +L  FLEFQ+P QWAP F ELPLG QRK+ +C 
Sbjct: 305 SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCA 364

Query: 413 TLQFAFMSPRMYIASTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMASLMSS 472
           +LQF+F  P++Y+ +T V   ++P+ G+RLYLEG++SN LA+H+QHL+SLPK+       
Sbjct: 365 SLQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKI----YQL 424

Query: 473 SDVASRCQWRGSDDGDCNGQFLEPVKWKRYSNVCTSAVRHDPNWLQQDGNGVYIVTGAQI 532
            D  +R   + S D     ++ E V WK YS+VCT  V  D        + + +VTGAQ+
Sbjct: 425 EDDLNRSIRQESHD----RRYYEKVNWKNYSHVCTEPVESD--------DDLSVVTGAQL 484

Query: 533 ISKGKWPKTILHLRLQYTHMPNCT-IRRTEWAAAPGASRKSTFLTNL-STTFSFTQRP-- 592
             +    K +L LRL ++ +   T ++ +EW  A G + KS  ++ L S  F+  Q+P  
Sbjct: 485 HVESHGFKNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPP 544

Query: 593 -AADKNKQVPGALNSGVYGEAPPVPVRSKKLLKYVDTTEVVRGPHDSPGHWLVTAAKLVT 652
             AD N      +NS +Y   PPVP ++ KLLK+VDT+E+ RGP +SPG+W+V+ A+L+ 
Sbjct: 545 RPADVN------INSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLV 590

Query: 653 ESGKIGLNVKFAL 656
           E GKI L VK++L
Sbjct: 605 EKGKISLKVKYSL 590

BLAST of MS000611 vs. TAIR 10
Match: AT1G28380.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 462.6 bits (1189), Expect = 5.2e-130
Identity = 257/602 (42.69%), Postives = 365/602 (60.63%), Query Frame = 0

Query: 63  RALECLGRGFDLTNDFRLKFVKGYGGGGRRLVVVDDSNRRDMVFPDGAVVRGVSQDIRCD 122
           +A+  +G G+DL +D R    K    G  RLV +D +  RD++FP G VV  VS  I+CD
Sbjct: 17  KAVSVIGLGYDLCSDVRFSACKTTPDGS-RLVEIDPTRNRDLIFPGGIVVNNVSSSIKCD 76

Query: 123 KGDRIRFKSDVLQFNQMSELLNQKSSIQGKVPSGYFNSMFGLSGDWFHDAADANHLAFDG 182
           KG+R R +SD+L FNQMSE  NQ   + GK+PSG FN+MF  S  W  DA+    LA+DG
Sbjct: 77  KGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLAYDG 136

Query: 183 YFIALYYLHLTASPLVLQDEVKKAVPSSWDPASLSRFIQTYGTHIIVGMAIGGQDLISVR 242
           +FI+LY + +    L L+DEVK+ VPSSWD A+L+ FI+ YGTH++VG+ +GG+D+I V+
Sbjct: 137 WFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIHVK 196

Query: 243 QRPSSPIPPAELRRNLEDLGDVLFS----DRRSPSALLKRDVKVPEIFTRILQ--SSSIQ 302
           Q   S   P E+++ L+  GD  F     + +SP+++     K   +    LQ   +S+ 
Sbjct: 197 QMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQWGLQPFGTSVS 256

Query: 303 LASISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSSKPEVILFKFVPITSLLTGVAGS 362
            A +  T +++ I  +C +RGG     SH  WL TVS  P VI   FVPITSLL+G+ G+
Sbjct: 257 SAVVMHTKNEE-IMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLSGLPGT 316

Query: 363 GYLSHAINLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQRKKATCPTLQFAFMSP 422
           G+LSHA+NLYLRYKP + +L  FLEFQ+P QWAP + +LPLG +R K + P+LQF+ M P
Sbjct: 317 GFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQFSLMGP 376

Query: 423 RMYIASTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMASLMSSSDVASRCQW 482
           ++Y+ +++V S  +P+ GLR +LEGKK N LA+H+QHL++ P    SL  S D       
Sbjct: 377 KLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACP---PSLHLSHDDTYEPIE 436

Query: 483 RGSDDGDCNGQFLEPVKWKRYSNVCTSAVRHDPNWLQQDGNGVYIVTGAQIISKGKWPKT 542
              + G     +  PVKW  +S+VCT  V++  N  + D +   IVT A +  KG   + 
Sbjct: 437 EPVEKG-----YYVPVKWGIFSHVCTYPVQY--NGARSD-DTASIVTKAWLEVKGMGMRK 496

Query: 543 ILHLRLQYTHMPNCTIRRTEWAAAPGASRKSTFLTNLSTTFS--FTQRPAADKNKQVPGA 602
           +L LRL ++   +   R++ W      SRKS   + +ST  S   +  PA  K  Q    
Sbjct: 497 VLFLRLGFSLDASAVTRKSCWDNLSTNSRKSGVFSMISTRLSTGLSPNPATTK-PQSKID 556

Query: 603 LNSGVYGEAPPVPVRSKKLLKYVDTTEVVRGPHDSPGHWLVTAAKLVTESGKIGLNVKFA 657
           +NS VY   P  PV+  KLL  VDT EV+RGP + PG+W+VT AKL  E+GKI +  K++
Sbjct: 557 INSAVYPRGPSPPVK-PKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYS 603

BLAST of MS000611 vs. TAIR 10
Match: AT1G29690.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 440.3 bits (1131), Expect = 2.8e-123
Identity = 241/598 (40.30%), Postives = 343/598 (57.36%), Query Frame = 0

Query: 64  ALECLGRGFDLTNDFRLKFVKGYGGGGRRLVVVDDSNRRDMVFPDGAVVRGVSQDIRCDK 123
           A++ LGRGFD+T+D RL + K  G  G RLV +++   RD+    G ++  V  DI C +
Sbjct: 24  AIQALGRGFDVTSDVRLLYCK--GAPGSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSR 83

Query: 124 GDRIRFKSDVLQFNQMSELLNQKSSIQGKVPSGYFNSMFGLSGDWFHDAADANHLAFDGY 183
           G+    +  V  F++M+E  N +S ++G +P G FN+MF  +G W  DAA    LA  GY
Sbjct: 84  GNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGY 143

Query: 184 FIALYYLHLTASPLVLQDEVKKAVPSSWDPASLSRFIQTYGTHIIVGMAIGGQDLISVRQ 243
           FI LY + L    LVL +E+++AVPSSWDPASL+ FI+ YGTHI+  + IGG+D++ +RQ
Sbjct: 144 FIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQ 203

Query: 244 RPSSPIPPAELRRNLEDLGDVLFSDRRSPSALLKRDVKVPEIFTRILQSSSIQLASISET 303
             SSP+P +E+   + D+                       I  R  ++ S  +    + 
Sbjct: 204 HQSSPLPVSEIENYVNDM-----------------------IKHRFHEAESQSITGPLKY 263

Query: 304 SSKDGITLICSKRGGDPFLHSHTSWLQTVSSKPEVILFKFVPITSLLTGVAGSGYLSHAI 363
             KD IT+I  +RGGD    SH  W +TV + P++I   F PI SLL GV G  +L+ AI
Sbjct: 264 KDKD-ITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHLTRAI 323

Query: 364 NLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQRKKATCPTLQFAFMSPRMYIAST 423
            LYL YKP + DLQYFL++QI   WAP    L    QRK+  C +LQF+ M P+++I++ 
Sbjct: 324 ELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGPKLFISAD 383

Query: 424 QVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMASLMSSSDVASRCQWRGSDDGD 483
           QVT  RKP+ GLRL LEG K N L++H+QHL SLPK++     S       +W+G ++ D
Sbjct: 384 QVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWDSHVPIGAPKWQGPEEQD 443

Query: 484 CNGQFLEPVKWKRYSNVCTSAVRHDPNWLQQDGNGVYIVTGAQIISKGKW---PKTILHL 543
              ++ EP+KWK +S+V TS + H    +  D +GV+IVTGAQ+   G W    K +LHL
Sbjct: 444 --SRWFEPIKWKNFSHVSTSPIEHTETHI-GDLSGVHIVTGAQL---GVWDFGSKNVLHL 503

Query: 544 RLQYTHMPNCTIRRTEWAAAPGASRKSTFLTNLSTTFSFTQRPAADKNKQVPGALNSGVY 603
           +L ++ +P CTIRR+ W                         P A   +  PG  ++   
Sbjct: 504 KLLFSKVPGCTIRRSVW----------------------DHTPVASSGRLEPGGPSTS-- 561

Query: 604 GEAPPVPVRSKKLLKYVDTTEVVRGPHDSPGHWLVTAAKLVTESGKIGLNVKFALLDY 659
                V  +S KL K VD++E+++GP D PGHWLVT AKL  E GKI L VK++LL+Y
Sbjct: 564 SSTEEVSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 561

BLAST of MS000611 vs. TAIR 10
Match: AT4G24290.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 271.6 bits (693), Expect = 1.7e-72
Identity = 147/336 (43.75%), Postives = 215/336 (63.99%), Query Frame = 0

Query: 53  MPASEKPVEIRALECLGRGFDLTNDFRLKFVKGYGGGGRRLVVVDDSNRRDMVFPDGAVV 112
           +PAS K  E+ A+  +G G+DL  D RLK+ KG     R L + +  +  ++V P G  +
Sbjct: 5   LPAS-KAAEV-AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISI 64

Query: 113 RGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQKSSIQGKVPSGYFNSMFGLSGDWFHDA 172
             VS+ I+CDKG+R+RF+SD+L F QM+E  NQ+ S+ GK+PSG FN+MF  S  W  DA
Sbjct: 65  PNVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDA 124

Query: 173 ADANHLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSSWDPASLSRFIQTYGTHIIVGMA 232
           A   +LAFDG FI+LY + L  S ++L++ VK+AVPS+WDPA+L+RFI  YGTHIIV + 
Sbjct: 125 AYTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVK 184

Query: 233 IGGQDLISVRQRPSSPIPPAELRRNLEDLGDVLFSD----RRSPSALLKRDVKVPEIFTR 292
           +GG+D+I  +Q+ SS + P +L++ L+++ D  F +      + S  ++   KV    T+
Sbjct: 185 MGGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVE---TK 244

Query: 293 ILQSSSIQLASISETSSKDGITLICSKRGGDPFLH-SHTSWLQTVSSKPEVILFKFVPIT 352
             +      +S+   ++K+    +C +RGG+   +  H  WLQTV  +P+VI   F+PIT
Sbjct: 245 EQRLRFADTSSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPIT 304

Query: 353 SLLTGVAGSGYLSHAINLYLRYKP--SLGDLQYFLE 382
           SLL GV GSG+LSHAINLYLR KP   L  + +FLE
Sbjct: 305 SLLNGVPGSGFLSHAINLYLRCKPINHLRFVHFFLE 335