MS000492 (gene) Bitter gourd (TR) v1

Overview


Name	MS000492
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Pectinesterase
Location	scaffold44: 2078616 .. 2081115 (+)
RNA-Seq Expression	MS000492
Synteny	MS000492

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCTACGGAAGGCTCAACGAGACCGACCCGGGCGGCACCTCCGGGCCCGGACCCGCCCACGATCACCTCCCCACCCCCAATAATCCAAGGAAGCGACGGTTTCTCCTTCTATCTCTCCTCTCCATCCTTCTAATCGTCGCCGCCGCCGTCTCCGCCACCTTCCTCGTCGGAATCCGAACCGCCTCCCACGGCCGCTCCGACCCGAATCTCCCTCTGAAGCCCACTCAGGCCATTTCCAAGGCCTGCAGCCGAACTCGCTACCCGACTCTCTGTGTCAACTCGCTCCTCGATTTCCCCGATTCACTCGACGCCGACGAGCACGATCTGGTCCACATTTCCTTCAACATCACCCTCCAGCGTCTCAACAAGGCCCTCTACTCCTCCTCCGAGATCTCCCCCCTTCAAATGGATCTCCGCGTCCGCTCCGCCTACGACGCCTGCCTCGAACTCCTCGATGACTCCGTCGACGCTCTCTCCCGCTCCCTGCAGTCCATCGCGCCCTCCTCCTCCTCCTCCGACGGCCGCCCTCAGCGCATCGGCTCCTCTCAGGACGTCATTACCTGGCTCAGCGCCGCCCTCACCAACCAAGACACCTGTACCGACGGATTCGCCGAACTCACCGGCGCCGTCAAGGATCAAATGACGGATAAACTCCACAACTTGTCGGAACTCGTCAGCAACTGCTTGGCGCTGTTCTCCGGCAGCGAAACCAGCGATTTCGACGGAGTGCCGATCCAGAACAAGCGGCGGCTGATGGAGGCCGCCGACGAGGAGTCCGGGAACTTCCCCCGGTGGATGAGTCGGAGGGAGAGGAGGTTGCTGACGATGCCGGTATCGGCGCTTCAGGCGGATATTGTGGTGTCTCAGAACGGGAGCGGAACGGTGAAGACGATCGCGGAAGCGATCAAAAAGGCGCCGCAGTACAGCAGCCGGCGGACGATCATTTACGTGATGGCAGGAAGGTAATTTTGTTTGCTCCTTCGTTTACTAACTACACTTCACACCCTTTTGACTTGCGCGTCACCGTCGTGAACTGTTTTCACGTGATTACATTGCAACCCACGCGCCATACACGTACTAATCGATTCTTGCTTGGCATTGGCGTCGCCCATTTACGAAAATATCCTCCTTACAGGTACGAAGAGAAGAATCTCAAGGTGGGGAGGAAGAAAACCAACTTGATGTTCGTCGGGGACGGGAAGGGTAAAACGGTCATTTCGGGTGATAAGAGCATCTTCGACAACGTGACGACTTTCCACACCGCGTCGTTTGGTAATAATTCTCCAAATTCAATTATTTATTAGTTATTTACTTCCATTCTTTTTTTTTTTTCTATTTCTTTATTTTTTGACCAGAATAATTTTCGTGGTTCTTAAAAATGACTTTTGCGTTCTTGAGAAAAAGAAAAAGAAAAATAAAAAAAGACAGCCCACGTGTCAATGCTCTACCGGGAGAGCCCTGATTGCATTCCCACGTGCCGCCTCATCATCTCCTTGATCTCCATGTAGACCAAATTACCCTTCTATTATTGCCCCAATTACCCTCTCTCCTTTTTAAAAATTAGCCTTTGTGGGCAAGAAGTGGAGGTCAGAACACATGCCACAATTTCAGATTCATTTCTCTAATTAAATTAAGCAATTAATCATATATATCAATTATATATTTATTCATTTATTAAACCCCACCATTTGGATTGGATATACACTGTTTAAATTAATCAATCTTCATGTCCACTGGTCAATTGCATGAGAATATCATTAAATTTTCGAGACCCGTCTGAGATCTTTTAATTTTCGTCACAGCTGCTACCGGCGCTGGGTTAATCTTGAGAGACATGACATTTGAGAACTGGGCCGGGCCGGGCCGGCACCAGGCAGTGGCGCTCCGGGTTGGCGCCGACCATGCCGTCGTGTACCGCTGCAACATAATCGGCTATCAGGACACTCTCTACGTCCATTCGAACCGCCAATTCTACCGGGAATGTGACATTTACGGGACCGTCGATTTTATCTTCGGCAATGCGGCGGTGGTGTTCCAAAACTGTAGCATTTACGCCCGAAAGGCCATGGATCTTCAGAAGAACACCATCACGGCCCAAAACCGGAAGGATCCGAATCAGAACACCGGCATCTCCATCCACGCCTGCCGGATTCTGGCCACCTCCGATCTCACGGCGTCCAATAGTAGCAACCCGACATATCTGGGCCGTCCGTGGAAATTATACTCTAGAGTCGTTTACATGCTATCATACATCGGCGGCCACGTGCACCCACACGGTTGGCTAGAGTGGAATGCCACGTTCGCTTTGGACACGCTGTATTACGGTGAGTATATGAATTATGGGCCGGGTGGGGCGGTGGGCCAGCGGGTCACGTGGCCCGGGTATCGGGTCATAACGTCCACGGTGGAAGCGAACAAGTTCACGGTGGCGCAGTTCATTTACGGCTCGTCATGGCTGCCGTCCACCGGAGTCGCGTTTCTCGGAGGGCTAAATGTG

mRNA sequence

ATGGGCTACGGAAGGCTCAACGAGACCGACCCGGGCGGCACCTCCGGGCCCGGACCCGCCCACGATCACCTCCCCACCCCCAATAATCCAAGGAAGCGACGGTTTCTCCTTCTATCTCTCCTCTCCATCCTTCTAATCGTCGCCGCCGCCGTCTCCGCCACCTTCCTCGTCGGAATCCGAACCGCCTCCCACGGCCGCTCCGACCCGAATCTCCCTCTGAAGCCCACTCAGGCCATTTCCAAGGCCTGCAGCCGAACTCGCTACCCGACTCTCTGTGTCAACTCGCTCCTCGATTTCCCCGATTCACTCGACGCCGACGAGCACGATCTGGTCCACATTTCCTTCAACATCACCCTCCAGCGTCTCAACAAGGCCCTCTACTCCTCCTCCGAGATCTCCCCCCTTCAAATGGATCTCCGCGTCCGCTCCGCCTACGACGCCTGCCTCGAACTCCTCGATGACTCCGTCGACGCTCTCTCCCGCTCCCTGCAGTCCATCGCGCCCTCCTCCTCCTCCTCCGACGGCCGCCCTCAGCGCATCGGCTCCTCTCAGGACGTCATTACCTGGCTCAGCGCCGCCCTCACCAACCAAGACACCTGTACCGACGGATTCGCCGAACTCACCGGCGCCGTCAAGGATCAAATGACGGATAAACTCCACAACTTGTCGGAACTCGTCAGCAACTGCTTGGCGCTGTTCTCCGGCAGCGAAACCAGCGATTTCGACGGAGTGCCGATCCAGAACAAGCGGCGGCTGATGGAGGCCGCCGACGAGGAGTCCGGGAACTTCCCCCGGTGGATGAGTCGGAGGGAGAGGAGGTTGCTGACGATGCCGGTATCGGCGCTTCAGGCGGATATTGTGGTGTCTCAGAACGGGAGCGGAACGGTGAAGACGATCGCGGAAGCGATCAAAAAGGCGCCGCAGTACAGCAGCCGGCGGACGATCATTTACGTGATGGCAGGAAGGTACGAAGAGAAGAATCTCAAGGTGGGGAGGAAGAAAACCAACTTGATGTTCGTCGGGGACGGGAAGGGTAAAACGGTCATTTCGGGTGATAAGAGCATCTTCGACAACGTGACGACTTTCCACACCGCGTCGTTTGCTGCTACCGGCGCTGGGTTAATCTTGAGAGACATGACATTTGAGAACTGGGCCGGGCCGGGCCGGCACCAGGCAGTGGCGCTCCGGGTTGGCGCCGACCATGCCGTCGTGTACCGCTGCAACATAATCGGCTATCAGGACACTCTCTACGTCCATTCGAACCGCCAATTCTACCGGGAATGTGACATTTACGGGACCGTCGATTTTATCTTCGGCAATGCGGCGGTGGTGTTCCAAAACTGTAGCATTTACGCCCGAAAGGCCATGGATCTTCAGAAGAACACCATCACGGCCCAAAACCGGAAGGATCCGAATCAGAACACCGGCATCTCCATCCACGCCTGCCGGATTCTGGCCACCTCCGATCTCACGGCGTCCAATAGTAGCAACCCGACATATCTGGGCCGTCCGTGGAAATTATACTCTAGAGTCGTTTACATGCTATCATACATCGGCGGCCACGTGCACCCACACGGTTGGCTAGAGTGGAATGCCACGTTCGCTTTGGACACGCTGTATTACGGTGAGTATATGAATTATGGGCCGGGTGGGGCGGTGGGCCAGCGGGTCACGTGGCCCGGGTATCGGGTCATAACGTCCACGGTGGAAGCGAACAAGTTCACGGTGGCGCAGTTCATTTACGGCTCGTCATGGCTGCCGTCCACCGGAGTCGCGTTTCTCGGAGGGCTAAATGTG

Coding sequence (CDS)

ATGGGCTACGGAAGGCTCAACGAGACCGACCCGGGCGGCACCTCCGGGCCCGGACCCGCCCACGATCACCTCCCCACCCCCAATAATCCAAGGAAGCGACGGTTTCTCCTTCTATCTCTCCTCTCCATCCTTCTAATCGTCGCCGCCGCCGTCTCCGCCACCTTCCTCGTCGGAATCCGAACCGCCTCCCACGGCCGCTCCGACCCGAATCTCCCTCTGAAGCCCACTCAGGCCATTTCCAAGGCCTGCAGCCGAACTCGCTACCCGACTCTCTGTGTCAACTCGCTCCTCGATTTCCCCGATTCACTCGACGCCGACGAGCACGATCTGGTCCACATTTCCTTCAACATCACCCTCCAGCGTCTCAACAAGGCCCTCTACTCCTCCTCCGAGATCTCCCCCCTTCAAATGGATCTCCGCGTCCGCTCCGCCTACGACGCCTGCCTCGAACTCCTCGATGACTCCGTCGACGCTCTCTCCCGCTCCCTGCAGTCCATCGCGCCCTCCTCCTCCTCCTCCGACGGCCGCCCTCAGCGCATCGGCTCCTCTCAGGACGTCATTACCTGGCTCAGCGCCGCCCTCACCAACCAAGACACCTGTACCGACGGATTCGCCGAACTCACCGGCGCCGTCAAGGATCAAATGACGGATAAACTCCACAACTTGTCGGAACTCGTCAGCAACTGCTTGGCGCTGTTCTCCGGCAGCGAAACCAGCGATTTCGACGGAGTGCCGATCCAGAACAAGCGGCGGCTGATGGAGGCCGCCGACGAGGAGTCCGGGAACTTCCCCCGGTGGATGAGTCGGAGGGAGAGGAGGTTGCTGACGATGCCGGTATCGGCGCTTCAGGCGGATATTGTGGTGTCTCAGAACGGGAGCGGAACGGTGAAGACGATCGCGGAAGCGATCAAAAAGGCGCCGCAGTACAGCAGCCGGCGGACGATCATTTACGTGATGGCAGGAAGGTACGAAGAGAAGAATCTCAAGGTGGGGAGGAAGAAAACCAACTTGATGTTCGTCGGGGACGGGAAGGGTAAAACGGTCATTTCGGGTGATAAGAGCATCTTCGACAACGTGACGACTTTCCACACCGCGTCGTTTGCTGCTACCGGCGCTGGGTTAATCTTGAGAGACATGACATTTGAGAACTGGGCCGGGCCGGGCCGGCACCAGGCAGTGGCGCTCCGGGTTGGCGCCGACCATGCCGTCGTGTACCGCTGCAACATAATCGGCTATCAGGACACTCTCTACGTCCATTCGAACCGCCAATTCTACCGGGAATGTGACATTTACGGGACCGTCGATTTTATCTTCGGCAATGCGGCGGTGGTGTTCCAAAACTGTAGCATTTACGCCCGAAAGGCCATGGATCTTCAGAAGAACACCATCACGGCCCAAAACCGGAAGGATCCGAATCAGAACACCGGCATCTCCATCCACGCCTGCCGGATTCTGGCCACCTCCGATCTCACGGCGTCCAATAGTAGCAACCCGACATATCTGGGCCGTCCGTGGAAATTATACTCTAGAGTCGTTTACATGCTATCATACATCGGCGGCCACGTGCACCCACACGGTTGGCTAGAGTGGAATGCCACGTTCGCTTTGGACACGCTGTATTACGGTGAGTATATGAATTATGGGCCGGGTGGGGCGGTGGGCCAGCGGGTCACGTGGCCCGGGTATCGGGTCATAACGTCCACGGTGGAAGCGAACAAGTTCACGGTGGCGCAGTTCATTTACGGCTCGTCATGGCTGCCGTCCACCGGAGTCGCGTTTCTCGGAGGGCTAAATGTG

Protein sequence

MGYGRLNETDPGGTSGPGPAHDHLPTPNNPRKRRFLLLSLLSILLIVAAAVSATFLVGIRTASHGRSDPNLPLKPTQAISKACSRTRYPTLCVNSLLDFPDSLDADEHDLVHISFNITLQRLNKALYSSSEISPLQMDLRVRSAYDACLELLDDSVDALSRSLQSIAPSSSSSDGRPQRIGSSQDVITWLSAALTNQDTCTDGFAELTGAVKDQMTDKLHNLSELVSNCLALFSGSETSDFDGVPIQNKRRLMEAADEESGNFPRWMSRRERRLLTMPVSALQADIVVSQNGSGTVKTIAEAIKKAPQYSSRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGDKSIFDNVTTFHTASFAATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGISIHACRILATSDLTASNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPHGWLEWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEANKFTVAQFIYGSSWLPSTGVAFLGGLNV

Homology

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BLAST of MS000492 vs. NCBI nr
Match: XP_023553011.1 (probable pectinesterase/pectinesterase inhibitor 34 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1065.1 bits (2753), Expect = 2.3e-307
Identity = 537/602 (89.20%), Postives = 562/602 (93.36%), Query Frame = 0


Query: 1   MGYGRLNETDPGGTSGPGPAH-DHLPTPNNPRKRRFLLLSLLSILLIVAAAVSATFLVGI 60
           MGYGRLNETDPGGTS   P+H  H     NPR+RR LL+SLLS++LIV AAVSATFLVG+
Sbjct: 1   MGYGRLNETDPGGTSSDAPSHLSHHAASPNPRRRRLLLISLLSLILIVTAAVSATFLVGL 60

Query: 61  RTASHGRSDPNLPLKPTQAISKACSRTRYPTLCVNSLLDFPDSLDADEHDLVHISFNITL 120
           RTAS  RSDPNLP KPTQAISKACSRTR+PTLCVNSLLDFP SL+A+E DLVHISFNITL
Sbjct: 61  RTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNITL 120

Query: 121 QRLNKALYSSSEISPLQMDLRVRSAYDACLELLDDSVDALSRSLQSIAPSSSSSDGRPQR 180
           Q LNKALY SSEIS LQMDLRVRSAYDAC+ELLDDSVDAL+RSLQS+AP  SSS+G PQR
Sbjct: 121 QHLNKALYFSSEISSLQMDLRVRSAYDACVELLDDSVDALTRSLQSVAP--SSSNGGPQR 180

Query: 181 IGSSQDVITWLSAALTNQDTCTDGFAELTGAVKDQMTDKLHNLSELVSNCLALFSGSETS 240
           +GSS DVITWLSAALTNQDTCTDGF++L+G+VKDQM DKLHNLSELVSNCLALFSGS TS
Sbjct: 181 LGSSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVTS 240

Query: 241 DFDGVPIQNKRRLMEAADEE--SGNFPRWMSRRERRLLTMPVSALQADIVVSQNGSGTVK 300
           DF GVPIQNKRRLM A D+E  SGNFP WMSRRERRLLT+PV ALQADIVVSQNGSGTVK
Sbjct: 241 DFSGVPIQNKRRLMNAGDDEDTSGNFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTVK 300

Query: 301 TIAEAIKKAPQYSSRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGDKSIFD 360
           TIAEAIKKAPQYS+RRTIIYVMAGRYEEKNLKVGRKK NLMFVGDGKGKTVISG KSIFD
Sbjct: 301 TIAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGGKSIFD 360

Query: 361 NVTTFHTASFAATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDTLY 420
           NVTTFHTASFAATGAG+ILRDMTFENWAGPGRHQAVALRVG DHAVVYRCNIIGYQDTLY
Sbjct: 361 NVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTLY 420

Query: 421 VHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGI 480
           VHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGI
Sbjct: 421 VHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGI 480

Query: 481 SIHACRILATSDLTASNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPHGWLEWNATFALD 540
           SIHACRILA SDL +SNSSNPTYLGRPWKLYSRVV+MLSYIG HVHP GWLEWNATFALD
Sbjct: 481 SIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFALD 540

Query: 541 TLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEANKFTVAQFIYGSSWLPSTGVAFLGGL 600
           TLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEA+KFTVAQFIYGSSWLPSTGVAFLGGL
Sbjct: 541 TLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGL 600

[Close]

BLAST of MS000492 vs. NCBI nr
Match: XP_022922767.1 (probable pectinesterase/pectinesterase inhibitor 34 [Cucurbita moschata])

HSP 1 Score: 1058.5 bits (2736), Expect = 2.1e-305
Identity = 534/602 (88.70%), Postives = 560/602 (93.02%), Query Frame = 0


Query: 1   MGYGRLNETDPGGTSGPGPAH-DHLPTPNNPRKRRFLLLSLLSILLIVAAAVSATFLVGI 60
           MGYGRLNETDPGGTS   P+H  H     NPR+ R LL+SLLS++LIVAAAVSATFLVG+
Sbjct: 1   MGYGRLNETDPGGTSSDAPSHLSHHAASPNPRRSRLLLISLLSLILIVAAAVSATFLVGL 60

Query: 61  RTASHGRSDPNLPLKPTQAISKACSRTRYPTLCVNSLLDFPDSLDADEHDLVHISFNITL 120
           RTAS  RSDPNLP KPTQAISKACSRTR+PTLCVNSLLDFP SL+A+E DLVHISFN+TL
Sbjct: 61  RTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNMTL 120

Query: 121 QRLNKALYSSSEISPLQMDLRVRSAYDACLELLDDSVDALSRSLQSIAPSSSSSDGRPQR 180
           Q LNKALY SS IS LQMDLRVRSAYDACLELLDDSVDAL+RSLQS+AP  SSS+G PQR
Sbjct: 121 QHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAP--SSSNGGPQR 180

Query: 181 IGSSQDVITWLSAALTNQDTCTDGFAELTGAVKDQMTDKLHNLSELVSNCLALFSGSETS 240
           +GSS DVITWLSAALTNQDTCTDGF++L+G+VKDQM DKLHNLSELVSNCLALFSGS TS
Sbjct: 181 LGSSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVTS 240

Query: 241 DFDGVPIQNKRRLMEAADEE--SGNFPRWMSRRERRLLTMPVSALQADIVVSQNGSGTVK 300
           DF GVPIQNKRRLM A + E  SGNFP WMSRRERRLLT+PV ALQADIVVSQNGSGTVK
Sbjct: 241 DFSGVPIQNKRRLMNAGENEDASGNFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTVK 300

Query: 301 TIAEAIKKAPQYSSRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGDKSIFD 360
           TIAEAIKKAPQYS+RRTIIYVMAGRYEEKNLKVGRKK NLMFVGDGKGKTVISG KSIFD
Sbjct: 301 TIAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGGKSIFD 360

Query: 361 NVTTFHTASFAATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDTLY 420
           NVTTFHTASFAATGAG+ILRDMTFENWAGPGRHQAVALRVG DHAVVYRCNIIGYQDTLY
Sbjct: 361 NVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTLY 420

Query: 421 VHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGI 480
           VHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNT+TAQNRKDPNQNTGI
Sbjct: 421 VHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTLTAQNRKDPNQNTGI 480

Query: 481 SIHACRILATSDLTASNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPHGWLEWNATFALD 540
           SIHACRILA SDL +SNSSNPTYLGRPWKLYSRVV+MLSYIG HVHP GWLEWNATFALD
Sbjct: 481 SIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFALD 540

Query: 541 TLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEANKFTVAQFIYGSSWLPSTGVAFLGGL 600
           TLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEA+KFTVAQFIYGSSWLPSTGVAFLGGL
Sbjct: 541 TLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGL 600

[Close]

BLAST of MS000492 vs. NCBI nr
Match: XP_038881267.1 (probable pectinesterase/pectinesterase inhibitor 34 [Benincasa hispida])

HSP 1 Score: 1056.2 bits (2730), Expect = 1.0e-304
Identity = 540/605 (89.26%), Postives = 562/605 (92.89%), Query Frame = 0


Query: 1   MGYGRLNETDPGGTSGPG-PAHDH---LPTPNNPRKRRFLLLSLLSILLIVAAAVSATFL 60
           MGYGRLNE+DPGGTS       DH    PTP+  R + F L+SLLS+LLIVAAAVSATFL
Sbjct: 1   MGYGRLNESDPGGTSSAAFQLSDHPISFPTPSRSRAQLF-LISLLSLLLIVAAAVSATFL 60

Query: 61  VGIRTASHGRSDPNLPLKPTQAISKACSRTRYPTLCVNSLLDFPDSLDADEHDLVHISFN 120
           +GIRTAS  RSD NLP KPTQAISKACSRTR+P+LCVNSLLDFP SL+A+E DLVHISFN
Sbjct: 61  IGIRTASPARSDRNLPRKPTQAISKACSRTRFPSLCVNSLLDFPGSLNANEQDLVHISFN 120

Query: 121 ITLQRLNKALYSSSEISPLQMDLRVRSAYDACLELLDDSVDALSRSLQSIAPSSSSSDGR 180
           ITLQ LNKALY SSEIS LQMDLRVRSAYDACLELLDDSVDAL+RSLQS+APSSSS  G 
Sbjct: 121 ITLQHLNKALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSS--GT 180

Query: 181 PQRIGSSQDVITWLSAALTNQDTCTDGFAELTGAVKDQMTDKLHNLSELVSNCLALFSGS 240
           PQR+GSS+DVITWLSAALTNQDTCTDGF+EL+G VK+QM DKLHNLSELVSNCLALFSGS
Sbjct: 181 PQRLGSSEDVITWLSAALTNQDTCTDGFSELSGTVKNQMADKLHNLSELVSNCLALFSGS 240

Query: 241 ETSDFDGVPIQNKRRLMEAADEE--SGNFPRWMSRRERRLLTMPVSALQADIVVSQNGSG 300
           ETSDF GVPIQNKRRLM A D E  SGNFP WMSRRERRLLT+PV ALQADIVVSQNG+G
Sbjct: 241 ETSDFSGVPIQNKRRLMNAGDNEDTSGNFPGWMSRRERRLLTLPVGALQADIVVSQNGNG 300

Query: 301 TVKTIAEAIKKAPQYSSRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGDKS 360
           TVKTIAEAIKKAPQYSSRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISG KS
Sbjct: 301 TVKTIAEAIKKAPQYSSRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGAKS 360

Query: 361 IFDNVTTFHTASFAATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQD 420
           IFDNVTTFHTASFAATGAG+ILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQD
Sbjct: 361 IFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQD 420

Query: 421 TLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQN 480
           TLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAM LQKNTITAQNRKDPNQN
Sbjct: 421 TLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMALQKNTITAQNRKDPNQN 480

Query: 481 TGISIHACRILATSDLTASNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPHGWLEWNATF 540
           TGISIHACRILATSDL +SNSSNPTYLGRPWKLYSRVVYMLSYIG HVHP GWLEWNATF
Sbjct: 481 TGISIHACRILATSDLASSNSSNPTYLGRPWKLYSRVVYMLSYIGSHVHPRGWLEWNATF 540

Query: 541 ALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEANKFTVAQFIYGSSWLPSTGVAFL 600
           ALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEA+KFTVAQFIYGSSWLPSTGVAFL
Sbjct: 541 ALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFL 600

[Close]

BLAST of MS000492 vs. NCBI nr
Match: XP_004146219.1 (probable pectinesterase/pectinesterase inhibitor 34 [Cucumis sativus] >KGN57675.1 hypothetical protein Csa_009694 [Cucumis sativus])

HSP 1 Score: 1053.9 bits (2724), Expect = 5.2e-304
Identity = 534/608 (87.83%), Postives = 560/608 (92.11%), Query Frame = 0


Query: 1   MGYGRLNETDPGGTSGPGPA------HDHLPTPNNPRKRRFLLLSLLSILLIVAAAVSAT 60
           MGYGRLNETDPGG S   PA        H PTPN  R  R LL+S LS+LLIVAAAVSAT
Sbjct: 1   MGYGRLNETDPGGPSHSSPASPPFQLSSHHPTPNR-RTTRLLLISFLSLLLIVAAAVSAT 60

Query: 61  FLVGIRTASHGRSDPNLPLKPTQAISKACSRTRYPTLCVNSLLDFPDSLDADEHDLVHIS 120
           FL+G+RTAS  RSDPNLP KPTQAISKACSRTR+PTLCVNSLLDFP SL+A+E DLVHIS
Sbjct: 61  FLIGLRTASPARSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHIS 120

Query: 121 FNITLQRLNKALYSSSEISPLQMDLRVRSAYDACLELLDDSVDALSRSLQSIAPSSSSSD 180
           FN+TLQ LNKALYSSSEIS LQMDLRVRSAYDACLELLDDS+DA+SRSLQS+APSSSSS 
Sbjct: 121 FNVTLQHLNKALYSSSEISSLQMDLRVRSAYDACLELLDDSIDAVSRSLQSVAPSSSSS- 180

Query: 181 GRPQRIGSSQDVITWLSAALTNQDTCTDGFAELTGAVKDQMTDKLHNLSELVSNCLALFS 240
             PQR+GSS+D+ITWLSAALTNQDTCTDGF+EL+G VK+QM DKLHNLSELVSNCLALFS
Sbjct: 181 -TPQRLGSSEDIITWLSAALTNQDTCTDGFSELSGTVKNQMADKLHNLSELVSNCLALFS 240

Query: 241 GSETSDFDGVPIQNKRRLMEAA---DEESGNFPRWMSRRERRLLTMPVSALQADIVVSQN 300
           GSETSDF GVPIQNKRRLME     ++ SG FPRWM+RRERRLLT+PV  LQADIVVSQN
Sbjct: 241 GSETSDFAGVPIQNKRRLMEEEGENEDSSGKFPRWMNRRERRLLTLPVGVLQADIVVSQN 300

Query: 301 GSGTVKTIAEAIKKAPQYSSRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISG 360
           G+GTVKTIAEAIKKAPQYSSRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISG
Sbjct: 301 GNGTVKTIAEAIKKAPQYSSRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISG 360

Query: 361 DKSIFDNVTTFHTASFAATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIG 420
            KSIFDNVTTFHTASFAATGAG+ILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIG
Sbjct: 361 SKSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIG 420

Query: 421 YQDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDP 480
           YQDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAM LQKNTITAQNRKDP
Sbjct: 421 YQDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMALQKNTITAQNRKDP 480

Query: 481 NQNTGISIHACRILATSDLTASNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPHGWLEWN 540
           NQNTGISIHACRILATSDL +SN+SNPTYLGRPWKLYSR V MLSYIG HVHP GWLEWN
Sbjct: 481 NQNTGISIHACRILATSDLESSNTSNPTYLGRPWKLYSRTVVMLSYIGNHVHPRGWLEWN 540

Query: 541 ATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEANKFTVAQFIYGSSWLPSTGV 600
           ATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEA+KFTVAQFIYGSSWLPSTGV
Sbjct: 541 ATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGV 600

[Close]

BLAST of MS000492 vs. NCBI nr
Match: XP_022984620.1 (probable pectinesterase/pectinesterase inhibitor 34 [Cucurbita maxima])

HSP 1 Score: 1052.7 bits (2721), Expect = 1.2e-303
Identity = 531/602 (88.21%), Postives = 559/602 (92.86%), Query Frame = 0


Query: 1   MGYGRLNETDPGGTSGPGPAH-DHLPTPNNPRKRRFLLLSLLSILLIVAAAVSATFLVGI 60
           M YGRLN+TDPGGTS   P+   H     NPR+RR +L+SLLS++LIVAAAVSATFLVG+
Sbjct: 1   MAYGRLNQTDPGGTSSDAPSQLSHHAVSPNPRRRRLILISLLSLILIVAAAVSATFLVGL 60

Query: 61  RTASHGRSDPNLPLKPTQAISKACSRTRYPTLCVNSLLDFPDSLDADEHDLVHISFNITL 120
           RTAS  RSDPNLP KPTQAISKACSRTR+PTLCVNSLLDFP SL+A+E DLVHISFN+TL
Sbjct: 61  RTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNMTL 120

Query: 121 QRLNKALYSSSEISPLQMDLRVRSAYDACLELLDDSVDALSRSLQSIAPSSSSSDGRPQR 180
           Q LNKALY SS IS LQMDLRVRSAYDACLELLDDSVDAL+RSLQS+AP  SSS+G PQR
Sbjct: 121 QHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAP--SSSNGGPQR 180

Query: 181 IGSSQDVITWLSAALTNQDTCTDGFAELTGAVKDQMTDKLHNLSELVSNCLALFSGSETS 240
           +GSS DVITWLSAALTNQDTCTDGF++L+G+VKDQM DKLHNLSELVSNCLALFSGS TS
Sbjct: 181 LGSSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVTS 240

Query: 241 DFDGVPIQNKRRLMEAADEE--SGNFPRWMSRRERRLLTMPVSALQADIVVSQNGSGTVK 300
           DF GVPIQNKRRLM A + E  SG+FP WMSRRERRLLT+PV ALQADIVVSQNGSGTVK
Sbjct: 241 DFSGVPIQNKRRLMNAGENEDTSGSFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTVK 300

Query: 301 TIAEAIKKAPQYSSRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGDKSIFD 360
           TIAEAIKKAPQYS+RRTIIYVMAGRYEEKNLKVGRKK NLMFVGDGKGKTVISG KSIFD
Sbjct: 301 TIAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKCNLMFVGDGKGKTVISGGKSIFD 360

Query: 361 NVTTFHTASFAATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDTLY 420
           NVTTFHTASFAATGAG+ILRDMTFENWAGPGRHQAVALRVG DHAVVYRCNIIGYQDTLY
Sbjct: 361 NVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTLY 420

Query: 421 VHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGI 480
           VHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGI
Sbjct: 421 VHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGI 480

Query: 481 SIHACRILATSDLTASNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPHGWLEWNATFALD 540
           SIHACRILA SDL +SNSSNPTYLGRPWKLYSRVV+MLSYIG HVHP GWLEWNATFALD
Sbjct: 481 SIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFALD 540

Query: 541 TLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEANKFTVAQFIYGSSWLPSTGVAFLGGL 600
           TLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEA+KFTVAQFIYGSSWLPSTGVAFLGGL
Sbjct: 541 TLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGL 600

[Close]

BLAST of MS000492 vs. ExPASy Swiss-Prot
Match: Q9M3B0 (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3702 GN=PME34 PE=2 SV=1)

HSP 1 Score: 786.6 bits (2030), Expect = 2.0e-226
Identity = 408/612 (66.67%), Postives = 473/612 (77.29%), Query Frame = 0


Query: 1   MGYGRLNETDPGG------TSGP---GPAHDHLPTPNNPRKRRFLLLSLLSILLIVAAAV 60
           MGY RL  +   G      T+ P     +    P  NN R ++ L++S  SI+L ++  +
Sbjct: 1   MGYERLGPSGATGSVTTSTTTAPILNQVSTSEQPENNNRRSKKKLVVS--SIVLAISLIL 60

Query: 61  SATFLVGIRT-ASHGRSDPNLPLKPTQAISKACSRTRYPTLCVNSLLDFPDSLDA-DEHD 120
           +A    G+R+     +S P L  KP+QAISKAC  TR+P LCV+SL+DFP SL A    D
Sbjct: 61  AAAIFAGVRSRLKLNQSVPGLARKPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKD 120

Query: 121 LVHISFNITLQRLNKALYSSSEISPLQMDLRVRSAYDACLELLDDSVDALSRSLQSIAPS 180
           L+H++ N+TL   + ALYSS+ +S + M  R RSAYD+C+ELLDDSVDALSR+L S+  S
Sbjct: 121 LIHVTVNMTLHHFSHALYSSASLSFVDMPPRARSAYDSCVELLDDSVDALSRALSSVVSS 180

Query: 181 SSSSDGRPQRIGSSQDVITWLSAALTNQDTCTDGFAEL-TGAVKDQMTDKLHNLSELVSN 240
           S+            QDV TWLSAALTN DTCT+GF  +  G VKD MT  L NLSELVSN
Sbjct: 181 SA----------KPQDVTTWLSAALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSN 240

Query: 241 CLALFSGS-ETSDFDGVPIQNKRRLMEAADEESGNFPRWMSRRERRLLTMPVSALQADIV 300
           CLA+FS S +  DF GVPIQN+R L    +E    FPRWM  +ER +L MPVS +QADI+
Sbjct: 241 CLAIFSASHDGDDFAGVPIQNRRLL--GVEEREEKFPRWMRPKEREILEMPVSQIQADII 300

Query: 301 VSQNGSGTVKTIAEAIKKAPQYSSRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKT 360
           VS++G+GT KTI+EAIKKAPQ S+RR IIYV AGRYEE NLKVGRKK NLMFVGDGKGKT
Sbjct: 301 VSKDGNGTCKTISEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKT 360

Query: 361 VISGDKSIFDNVTTFHTASFAATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRC 420
           VISG KSIFDN+TTFHTASFAATGAG I RD+TFENWAGP +HQAVALR+GADHAV+YRC
Sbjct: 361 VISGGKSIFDNITTFHTASFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRC 420

Query: 421 NIIGYQDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQN 480
           NIIGYQDTLYVHSNRQF+RECDIYGTVDFIFGNAAVV QNCSIYARK MD QKNTITAQN
Sbjct: 421 NIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQN 480

Query: 481 RKDPNQNTGISIHACRILATSDLTASNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPHGW 540
           RKDPNQNTGISIHA R+LA SDL A+N S  TYLGRPWKL+SR VYM+SYIGGHVH  GW
Sbjct: 481 RKDPNQNTGISIHASRVLAASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGW 540

Query: 541 LEWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEANKFTVAQFIYGSSWLP 600
           LEWN TFALDTLYYGEY+N GPG  +GQRV+WPGYRVI ST EAN+FTVA+FIYGSSWLP
Sbjct: 541 LEWNTTFALDTLYYGEYLNSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLP 598

[Close]

BLAST of MS000492 vs. ExPASy Swiss-Prot
Match: Q9FK05 (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3702 GN=PME61 PE=2 SV=1)

HSP 1 Score: 765.8 bits (1976), Expect = 3.7e-220
Identity = 389/601 (64.73%), Postives = 463/601 (77.04%), Query Frame = 0


Query: 1   MGYGRLNETDPGGTSGPGPAHDHLPTPNNPRKRRFLLLSLLSILLIVAAAVSATFLVGIR 60
           MGY RL    P G S P         P   +K +  L+ L ++ ++V   V      GIR
Sbjct: 1   MGYDRLG---PSGPSNPNQKDPATSLPELQKKTKTKLI-LFTLAVLVVGVVCFGIFAGIR 60

Query: 61  TASHGRSDPNLPLKPTQAISKACSRTRYPTLCVNSLLDFPDSLDADEHDLVHISFNITLQ 120
               G+++P L  KPTQAIS+ CS++ YP LC+++LLDFP SL ADE++L+HISFN TLQ
Sbjct: 61  AVDSGKTEPKLTRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQ 120

Query: 121 RLNKALYSSSEISPLQMDLRVRSAYDACLELLDDSVDALSRSLQSIAPSSSSSDGRPQRI 180
           + +KALY+SS I+  QM  RVRSAYD+CLELLDDSVDAL+R+L S+   S          
Sbjct: 121 KFSKALYTSSTITYTQMPPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDE------- 180

Query: 181 GSSQDVITWLSAALTNQDTCTDGFAELT---GAVKDQMTDKLHNLSELVSNCLALFSGSE 240
            S  DV+TWLS+A+TN DTCTDGF E+    G VKDQ+   + +LSE+VSNCLA+F+G +
Sbjct: 181 -SHSDVMTWLSSAMTNHDTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAG-K 240

Query: 241 TSDFDGVPIQNKRRLMEAADEESGNFPRWMSRRERRLLTMPVSALQADIVVSQNGSGTVK 300
             D  GVP+ N R+L+    EE+   P W+ R +R LL  P SA+QADI VS++GSGT K
Sbjct: 241 VKDLSGVPVVNNRKLL--GTEETEELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFK 300

Query: 301 TIAEAIKKAPQYSSRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGDKSIFD 360
           TIAEAIKKAP++SSRR +IYV AGRYEE+NLKVGRKKTNLMF+GDGKGKTVI+G KSI D
Sbjct: 301 TIAEAIKKAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIAD 360

Query: 361 NVTTFHTASFAATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDTLY 420
           ++TTFHTA+FAATGAG I+RDMTFEN+AGP +HQAVALRVG DHAVVYRCNIIGYQD LY
Sbjct: 361 DLTTFHTATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALY 420

Query: 421 VHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGI 480
           VHSNRQF+REC+IYGTVDFIFGNAAV+ Q+C+IYARK M  QK TITAQNRKDPNQNTGI
Sbjct: 421 VHSNRQFFRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGI 480

Query: 481 SIHACRILATSDLTASNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPHGWLEWNATFALD 540
           SIHAC++LAT DL AS  S PTYLGRPWKLYSRVVYM+S +G H+ P GWLEWN  FALD
Sbjct: 481 SIHACKLLATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALD 540

Query: 541 TLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEANKFTVAQFIYGSSWLPSTGVAFLGGL 599
           +LYYGEYMN G G  +GQRV WPGY VITSTVEA+KFTVAQFI GSSWLPSTGV+F  GL
Sbjct: 541 SLYYGEYMNKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGL 586

[Close]

BLAST of MS000492 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 1.3e-124
Identity = 252/579 (43.52%), Postives = 355/579 (61.31%), Query Frame = 0


Query: 28  NNPRKRRFLLLSLLSILLIVAAAVSATFLVGIRTASHGRSDPNLPLKPTQAISK-ACSRT 87
           N P+K++ L L+L + LL+VAA +    + G+ +  +   + N   +P  AI K +CS T
Sbjct: 22  NIPKKKKKLFLALFATLLVVAAVIG--IVAGVNSRKNSGDNGN---EPHHAILKSSCSST 81

Query: 88  RYPTLCVNSLLDFPDSLD--ADEHDLVHISFNITLQRLNKALYSSSE-ISPLQMDLRVRS 147
           RYP LC +++   P++      + D++ +S NIT   +    +   + +    +  R + 
Sbjct: 82  RYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREKV 141

Query: 148 AYDACLELLDDSVDALSRSLQSIAPSSSSSDGRPQRIGSSQ---DVITWLSAALTNQDTC 207
           A   CLE +D+++D L ++++ +       +  P +   SQ   D+ T +SAA+TNQ TC
Sbjct: 142 ALHDCLETIDETLDELHKAVEDL-------EEYPNKKSLSQHADDLKTLMSAAMTNQGTC 201

Query: 208 TDGFA--ELTGAVKDQMTDKLHNLSELVSNCLALFSGSETSDFDGVPIQNKRRLMEAADE 267
            DGF+  +    V+D ++D   ++ ++ SN LA+      +D   +   N R+L+E    
Sbjct: 202 LDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKLIEETST 261

Query: 268 ESGNFPRWMSRRERRLLTMPVSALQADIVVSQNGSGTVKTIAEAIKKAPQYSSRRTIIYV 327
             G +P W+S  +RRLL    S++  ++VV+ +GSG  KT+A ++  APQ  ++R II +
Sbjct: 262 VDG-WPAWLSTGDRRLLQS--SSVTPNVVVAADGSGNFKTVAASVAAAPQGGTKRYIIRI 321

Query: 328 MAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGDKSIFDNVTTFHTASFAATGAGLILRD 387
            AG Y E N++V +K  N+MF+GDG+ +T+I+G +++ D  TTF +A+ A  G G + RD
Sbjct: 322 KAGVYRE-NVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVAVVGEGFLARD 381

Query: 388 MTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDTLYVHSNRQFYRECDIYGTVDFIF 447
           +TF+N AGP +HQAVALRVGAD +  Y C+++ YQDTLYVHSNRQF+  C I GTVDFIF
Sbjct: 382 ITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIF 441

Query: 448 GNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGISIHACRILATSDLTASNSSNP 507
           GNAA V QNC I+ARK    QKN +TAQ R DPNQNTGI I   RI ATSDL     S P
Sbjct: 442 GNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFP 501

Query: 508 TYLGRPWKLYSRVVYMLSYIGGHVHPHGWLEWNATFALDTLYYGEYMNYGPGGAVGQRVT 567
           TYLGRPWK YSR V M S I   +HP GW EW+  FAL+TL+YGE+ N G G     RV 
Sbjct: 502 TYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVK 561

Query: 568 WPGYRVITSTVEANKFTVAQFIYGSSWLPSTGVAFLGGL 598
           W G+RVITS  EA  FT   FI GSSWL STG  F  GL
Sbjct: 562 WKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

[Close]

BLAST of MS000492 vs. ExPASy Swiss-Prot
Match: O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 5.0e-124
Identity = 253/579 (43.70%), Postives = 353/579 (60.97%), Query Frame = 0


Query: 28  NNPRKRRFLLLSLLSILLIVAAAVSATFLVGIRTASHGRSDPNLPLKPTQAISK-ACSRT 87
           N P+K++ L L+L + LL+VAA +    + G+ +  +   + N   +P  AI K +CS T
Sbjct: 22  NIPKKKKKLFLALFATLLVVAAVIG--IVAGVNSRKNSGDNGN---EPHHAILKSSCSST 81

Query: 88  RYPTLCVNSLLDFPDSLD--ADEHDLVHISFNITLQRLNKALYSSSE-ISPLQMDLRVRS 147
           RYP LC +++   P++      + D++ +S NIT   +    +   + +    +  R + 
Sbjct: 82  RYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREKV 141

Query: 148 AYDACLELLDDSVDALSRSLQSIAPSSSSSDGRPQRIGSSQ---DVITWLSAALTNQDTC 207
           A   CLE +D+++D L ++++ +       +  P +   SQ   D+ T +SAA+TNQ TC
Sbjct: 142 ALHDCLETIDETLDELHKAVEDL-------EEYPNKKSLSQHADDLKTLMSAAMTNQGTC 201

Query: 208 TDGFA--ELTGAVKDQMTDKLHNLSELVSNCLALFSGSETSDFDGVPIQNKRRLMEAADE 267
            DGF+  +    V+D ++D   ++ ++ SN LA+      +D   +   N R+L E    
Sbjct: 202 LDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKLTEETST 261

Query: 268 ESGNFPRWMSRRERRLLTMPVSALQADIVVSQNGSGTVKTIAEAIKKAPQYSSRRTIIYV 327
             G +P W+S  +RRLL    S++  + VV+ +GSG  KT+A A+  APQ  ++R II +
Sbjct: 262 VDG-WPAWLSPGDRRLLQS--SSVTPNAVVAADGSGNFKTVAAAVAAAPQGGTKRYIIRI 321

Query: 328 MAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGDKSIFDNVTTFHTASFAATGAGLILRD 387
            AG Y E N++V +K  N+MF+GDG+ +T+I+G +++ D  TTF +A+ A  G G + RD
Sbjct: 322 KAGVYRE-NVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAAVVGEGFLARD 381

Query: 388 MTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDTLYVHSNRQFYRECDIYGTVDFIF 447
           +TF+N AGP +HQAVALRVGAD +  Y C+++ YQDTLYVHSNRQF+  C I GTVDFIF
Sbjct: 382 ITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIF 441

Query: 448 GNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGISIHACRILATSDLTASNSSNP 507
           GNAA V QNC I+ARK    QKN +TAQ R DPNQNTGI I   RI ATSDL     S P
Sbjct: 442 GNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFP 501

Query: 508 TYLGRPWKLYSRVVYMLSYIGGHVHPHGWLEWNATFALDTLYYGEYMNYGPGGAVGQRVT 567
           TYLGRPWK YSR V M S I   +HP GW EW+  FAL+TL+YGE+ N G G     RV 
Sbjct: 502 TYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVK 561

Query: 568 WPGYRVITSTVEANKFTVAQFIYGSSWLPSTGVAFLGGL 598
           W G+RVITS  EA  FT   FI GSSWL STG  F  GL
Sbjct: 562 WKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

[Close]

BLAST of MS000492 vs. ExPASy Swiss-Prot
Match: Q43062 (Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 436.8 bits (1122), Expect = 3.9e-121
Identity = 229/464 (49.35%), Postives = 298/464 (64.22%), Query Frame = 0


Query: 138 DLRVRSAYDACLELLDDSVDALSRSLQSIAPSSSSSDGRPQRIGS-SQDVITWLSAALTN 197
           D R+ +A   CL+LLD S D L+ SL     +S +  G+    G  S D+ TWLSAAL N
Sbjct: 83  DFRLANAISDCLDLLDFSADELNWSLS----ASQNQKGKNNSTGKLSSDLRTWLSAALVN 142

Query: 198 QDTCTDGFAELTGAVKDQMTDKLHNLSELVSNCLALFSGSETSDFDGVPIQNKRRLMEAA 257
           QDTC++GF      V+  ++  L  ++ LV   L              P  N++      
Sbjct: 143 QDTCSNGFEGTNSIVQGLISAGLGQVTSLVQELLTQVH----------PNSNQQ------ 202

Query: 258 DEESGNFPRWMSRRERRLLTMPVSALQADIVVSQNGSGTVKTIAEAIKKAPQYSSRRTII 317
              +G  P W+  ++R+LL      +  D +V+Q+G+G    + +A+  AP YS RR +I
Sbjct: 203 -GPNGQIPSWVKTKDRKLL--QADGVSVDAIVAQDGTGNFTNVTDAVLAAPDYSMRRYVI 262

Query: 318 YVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGDKSIFDNVTTFHTASFAATGAGLIL 377
           Y+  G Y+E N+++ +KK NLM +GDG   T+ISG++S  D  TTF +A+FA +G G I 
Sbjct: 263 YIKRGTYKE-NVEIKKKKWNLMMIGDGMDATIISGNRSFVDGWTTFRSATFAVSGRGFIA 322

Query: 378 RDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDTLYVHSNRQFYRECDIYGTVDF 437
           RD+TFEN AGP +HQAVALR  +D +V YRCNI GYQDTLY H+ RQFYR+C I GTVDF
Sbjct: 323 RDITFENTAGPEKHQAVALRSDSDLSVFYRCNIRGYQDTLYTHTMRQFYRDCKISGTVDF 382

Query: 438 IFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGISIHACRILATSDL-TASNS 497
           IFG+A VVFQNC I A+K +  QKN+ITAQ RKDPN+ TGISI  C I A SDL  AS +
Sbjct: 383 IFGDATVVFQNCQILAKKGLPNQKNSITAQGRKDPNEPTGISIQFCNITADSDLEAASVN 442

Query: 498 SNPTYLGRPWKLYSRVVYMLSYIGGHVHPHGWLEWNATFALDTLYYGEYMNYGPGGAVGQ 557
           S PTYLGRPWKLYSR V M S++   + P GWLEWN  FAL++L+YGEYMNYGPG  +G 
Sbjct: 443 STPTYLGRPWKLYSRTVIMQSFLSNVIRPEGWLEWNGDFALNSLFYGEYMNYGPGAGLGS 502

Query: 558 RVTWPGYRVITSTVEANKFTVAQFIYGSSWLPSTGVAFLGGLNV 600
           RV WPGY+V   + +A  +TVAQFI G+ WLPSTGV +     V
Sbjct: 503 RVKWPGYQVFNESTQAKNYTVAQFIEGNLWLPSTGVKYTAEFGV 522

[Close]

BLAST of MS000492 vs. ExPASy TrEMBL
Match: A0A6J1E487 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430666 PE=3 SV=1)

HSP 1 Score: 1058.5 bits (2736), Expect = 1.0e-305
Identity = 534/602 (88.70%), Postives = 560/602 (93.02%), Query Frame = 0


Query: 1   MGYGRLNETDPGGTSGPGPAH-DHLPTPNNPRKRRFLLLSLLSILLIVAAAVSATFLVGI 60
           MGYGRLNETDPGGTS   P+H  H     NPR+ R LL+SLLS++LIVAAAVSATFLVG+
Sbjct: 1   MGYGRLNETDPGGTSSDAPSHLSHHAASPNPRRSRLLLISLLSLILIVAAAVSATFLVGL 60

Query: 61  RTASHGRSDPNLPLKPTQAISKACSRTRYPTLCVNSLLDFPDSLDADEHDLVHISFNITL 120
           RTAS  RSDPNLP KPTQAISKACSRTR+PTLCVNSLLDFP SL+A+E DLVHISFN+TL
Sbjct: 61  RTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNMTL 120

Query: 121 QRLNKALYSSSEISPLQMDLRVRSAYDACLELLDDSVDALSRSLQSIAPSSSSSDGRPQR 180
           Q LNKALY SS IS LQMDLRVRSAYDACLELLDDSVDAL+RSLQS+AP  SSS+G PQR
Sbjct: 121 QHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAP--SSSNGGPQR 180

Query: 181 IGSSQDVITWLSAALTNQDTCTDGFAELTGAVKDQMTDKLHNLSELVSNCLALFSGSETS 240
           +GSS DVITWLSAALTNQDTCTDGF++L+G+VKDQM DKLHNLSELVSNCLALFSGS TS
Sbjct: 181 LGSSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVTS 240

Query: 241 DFDGVPIQNKRRLMEAADEE--SGNFPRWMSRRERRLLTMPVSALQADIVVSQNGSGTVK 300
           DF GVPIQNKRRLM A + E  SGNFP WMSRRERRLLT+PV ALQADIVVSQNGSGTVK
Sbjct: 241 DFSGVPIQNKRRLMNAGENEDASGNFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTVK 300

Query: 301 TIAEAIKKAPQYSSRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGDKSIFD 360
           TIAEAIKKAPQYS+RRTIIYVMAGRYEEKNLKVGRKK NLMFVGDGKGKTVISG KSIFD
Sbjct: 301 TIAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGGKSIFD 360

Query: 361 NVTTFHTASFAATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDTLY 420
           NVTTFHTASFAATGAG+ILRDMTFENWAGPGRHQAVALRVG DHAVVYRCNIIGYQDTLY
Sbjct: 361 NVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTLY 420

Query: 421 VHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGI 480
           VHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNT+TAQNRKDPNQNTGI
Sbjct: 421 VHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTLTAQNRKDPNQNTGI 480

Query: 481 SIHACRILATSDLTASNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPHGWLEWNATFALD 540
           SIHACRILA SDL +SNSSNPTYLGRPWKLYSRVV+MLSYIG HVHP GWLEWNATFALD
Sbjct: 481 SIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFALD 540

Query: 541 TLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEANKFTVAQFIYGSSWLPSTGVAFLGGL 600
           TLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEA+KFTVAQFIYGSSWLPSTGVAFLGGL
Sbjct: 541 TLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGL 600

[Close]

BLAST of MS000492 vs. ExPASy TrEMBL
Match: A0A0A0L6S0 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G239880 PE=3 SV=1)

HSP 1 Score: 1053.9 bits (2724), Expect = 2.5e-304
Identity = 534/608 (87.83%), Postives = 560/608 (92.11%), Query Frame = 0


Query: 1   MGYGRLNETDPGGTSGPGPA------HDHLPTPNNPRKRRFLLLSLLSILLIVAAAVSAT 60
           MGYGRLNETDPGG S   PA        H PTPN  R  R LL+S LS+LLIVAAAVSAT
Sbjct: 1   MGYGRLNETDPGGPSHSSPASPPFQLSSHHPTPNR-RTTRLLLISFLSLLLIVAAAVSAT 60

Query: 61  FLVGIRTASHGRSDPNLPLKPTQAISKACSRTRYPTLCVNSLLDFPDSLDADEHDLVHIS 120
           FL+G+RTAS  RSDPNLP KPTQAISKACSRTR+PTLCVNSLLDFP SL+A+E DLVHIS
Sbjct: 61  FLIGLRTASPARSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHIS 120

Query: 121 FNITLQRLNKALYSSSEISPLQMDLRVRSAYDACLELLDDSVDALSRSLQSIAPSSSSSD 180
           FN+TLQ LNKALYSSSEIS LQMDLRVRSAYDACLELLDDS+DA+SRSLQS+APSSSSS 
Sbjct: 121 FNVTLQHLNKALYSSSEISSLQMDLRVRSAYDACLELLDDSIDAVSRSLQSVAPSSSSS- 180

Query: 181 GRPQRIGSSQDVITWLSAALTNQDTCTDGFAELTGAVKDQMTDKLHNLSELVSNCLALFS 240
             PQR+GSS+D+ITWLSAALTNQDTCTDGF+EL+G VK+QM DKLHNLSELVSNCLALFS
Sbjct: 181 -TPQRLGSSEDIITWLSAALTNQDTCTDGFSELSGTVKNQMADKLHNLSELVSNCLALFS 240

Query: 241 GSETSDFDGVPIQNKRRLMEAA---DEESGNFPRWMSRRERRLLTMPVSALQADIVVSQN 300
           GSETSDF GVPIQNKRRLME     ++ SG FPRWM+RRERRLLT+PV  LQADIVVSQN
Sbjct: 241 GSETSDFAGVPIQNKRRLMEEEGENEDSSGKFPRWMNRRERRLLTLPVGVLQADIVVSQN 300

Query: 301 GSGTVKTIAEAIKKAPQYSSRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISG 360
           G+GTVKTIAEAIKKAPQYSSRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISG
Sbjct: 301 GNGTVKTIAEAIKKAPQYSSRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISG 360

Query: 361 DKSIFDNVTTFHTASFAATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIG 420
            KSIFDNVTTFHTASFAATGAG+ILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIG
Sbjct: 361 SKSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIG 420

Query: 421 YQDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDP 480
           YQDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAM LQKNTITAQNRKDP
Sbjct: 421 YQDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMALQKNTITAQNRKDP 480

Query: 481 NQNTGISIHACRILATSDLTASNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPHGWLEWN 540
           NQNTGISIHACRILATSDL +SN+SNPTYLGRPWKLYSR V MLSYIG HVHP GWLEWN
Sbjct: 481 NQNTGISIHACRILATSDLESSNTSNPTYLGRPWKLYSRTVVMLSYIGNHVHPRGWLEWN 540

Query: 541 ATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEANKFTVAQFIYGSSWLPSTGV 600
           ATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEA+KFTVAQFIYGSSWLPSTGV
Sbjct: 541 ATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGV 600

[Close]

BLAST of MS000492 vs. ExPASy TrEMBL
Match: A0A6J1J930 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111482853 PE=3 SV=1)

HSP 1 Score: 1052.7 bits (2721), Expect = 5.6e-304
Identity = 531/602 (88.21%), Postives = 559/602 (92.86%), Query Frame = 0


Query: 1   MGYGRLNETDPGGTSGPGPAH-DHLPTPNNPRKRRFLLLSLLSILLIVAAAVSATFLVGI 60
           M YGRLN+TDPGGTS   P+   H     NPR+RR +L+SLLS++LIVAAAVSATFLVG+
Sbjct: 1   MAYGRLNQTDPGGTSSDAPSQLSHHAVSPNPRRRRLILISLLSLILIVAAAVSATFLVGL 60

Query: 61  RTASHGRSDPNLPLKPTQAISKACSRTRYPTLCVNSLLDFPDSLDADEHDLVHISFNITL 120
           RTAS  RSDPNLP KPTQAISKACSRTR+PTLCVNSLLDFP SL+A+E DLVHISFN+TL
Sbjct: 61  RTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNMTL 120

Query: 121 QRLNKALYSSSEISPLQMDLRVRSAYDACLELLDDSVDALSRSLQSIAPSSSSSDGRPQR 180
           Q LNKALY SS IS LQMDLRVRSAYDACLELLDDSVDAL+RSLQS+AP  SSS+G PQR
Sbjct: 121 QHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAP--SSSNGGPQR 180

Query: 181 IGSSQDVITWLSAALTNQDTCTDGFAELTGAVKDQMTDKLHNLSELVSNCLALFSGSETS 240
           +GSS DVITWLSAALTNQDTCTDGF++L+G+VKDQM DKLHNLSELVSNCLALFSGS TS
Sbjct: 181 LGSSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVTS 240

Query: 241 DFDGVPIQNKRRLMEAADEE--SGNFPRWMSRRERRLLTMPVSALQADIVVSQNGSGTVK 300
           DF GVPIQNKRRLM A + E  SG+FP WMSRRERRLLT+PV ALQADIVVSQNGSGTVK
Sbjct: 241 DFSGVPIQNKRRLMNAGENEDTSGSFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTVK 300

Query: 301 TIAEAIKKAPQYSSRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGDKSIFD 360
           TIAEAIKKAPQYS+RRTIIYVMAGRYEEKNLKVGRKK NLMFVGDGKGKTVISG KSIFD
Sbjct: 301 TIAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKCNLMFVGDGKGKTVISGGKSIFD 360

Query: 361 NVTTFHTASFAATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDTLY 420
           NVTTFHTASFAATGAG+ILRDMTFENWAGPGRHQAVALRVG DHAVVYRCNIIGYQDTLY
Sbjct: 361 NVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTLY 420

Query: 421 VHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGI 480
           VHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGI
Sbjct: 421 VHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGI 480

Query: 481 SIHACRILATSDLTASNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPHGWLEWNATFALD 540
           SIHACRILA SDL +SNSSNPTYLGRPWKLYSRVV+MLSYIG HVHP GWLEWNATFALD
Sbjct: 481 SIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFALD 540

Query: 541 TLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEANKFTVAQFIYGSSWLPSTGVAFLGGL 600
           TLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEA+KFTVAQFIYGSSWLPSTGVAFLGGL
Sbjct: 541 TLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGL 600

[Close]

BLAST of MS000492 vs. ExPASy TrEMBL
Match: A0A5A7U3N7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold175G001450 PE=3 SV=1)

HSP 1 Score: 1048.9 bits (2711), Expect = 8.1e-303
Identity = 532/608 (87.50%), Postives = 559/608 (91.94%), Query Frame = 0


Query: 1   MGYGRLNETDPGGTSGPGPAHD------HLPTPNNPRKRRFLLLSLLSILLIVAAAVSAT 60
           MGYGRLNETDPGG S             H PTPN     R LL+S LS+LLIVAAAVSAT
Sbjct: 1   MGYGRLNETDPGGPSDSSSVASAFQLSAHHPTPNR-STTRLLLISFLSLLLIVAAAVSAT 60

Query: 61  FLVGIRTASHGRSDPNLPLKPTQAISKACSRTRYPTLCVNSLLDFPDSLDADEHDLVHIS 120
           FL+G+RTAS  RSDPNLP KPTQAISKACSRTR+PTLCVNSLLDFP SL+A+E DLVHIS
Sbjct: 61  FLIGLRTASPARSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHIS 120

Query: 121 FNITLQRLNKALYSSSEISPLQMDLRVRSAYDACLELLDDSVDALSRSLQSIAPSSSSSD 180
           FNITLQ LNKALYSSSEIS LQMDLRVRSAYDACLELLDDS+DA+SRSLQS+APSSSSS 
Sbjct: 121 FNITLQHLNKALYSSSEISSLQMDLRVRSAYDACLELLDDSIDAVSRSLQSVAPSSSSS- 180

Query: 181 GRPQRIGSSQDVITWLSAALTNQDTCTDGFAELTGAVKDQMTDKLHNLSELVSNCLALFS 240
             PQR+GSS+D+ITWLSAALTNQDTCTDGF+EL+G VK+QM DKLHNLSELVSNCLALFS
Sbjct: 181 -TPQRLGSSEDIITWLSAALTNQDTCTDGFSELSGTVKNQMADKLHNLSELVSNCLALFS 240

Query: 241 GSETSDFDGVPIQNKRRLMEAA---DEESGNFPRWMSRRERRLLTMPVSALQADIVVSQN 300
           GSETSDF GVPIQNKRRLM+     ++ SGNFPRWM  RERRLLT+PV  LQADIVVSQN
Sbjct: 241 GSETSDFAGVPIQNKRRLMKEEGENEDSSGNFPRWMRGRERRLLTLPVGVLQADIVVSQN 300

Query: 301 GSGTVKTIAEAIKKAPQYSSRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISG 360
           G+GTVKTIAEAIKKAPQYS+RRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISG
Sbjct: 301 GNGTVKTIAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISG 360

Query: 361 DKSIFDNVTTFHTASFAATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIG 420
            KSIFDNVTTFHTASFAATGAG+ILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIG
Sbjct: 361 SKSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIG 420

Query: 421 YQDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDP 480
           YQDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAM LQKNTITAQNRKDP
Sbjct: 421 YQDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMALQKNTITAQNRKDP 480

Query: 481 NQNTGISIHACRILATSDLTASNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPHGWLEWN 540
           NQNTGISIHACRILATSDL +SN+SNPTYLGRPWKLYSRVV+MLSYIGGHVHP GWLEWN
Sbjct: 481 NQNTGISIHACRILATSDLQSSNTSNPTYLGRPWKLYSRVVFMLSYIGGHVHPRGWLEWN 540

Query: 541 ATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEANKFTVAQFIYGSSWLPSTGV 600
           ATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEA+KFTVAQFIYGSSWLPSTGV
Sbjct: 541 ATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGV 600

[Close]

BLAST of MS000492 vs. ExPASy TrEMBL
Match: A0A6J1G0F6 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111449561 PE=3 SV=1)

HSP 1 Score: 931.8 bits (2407), Expect = 1.4e-267
Identity = 479/603 (79.44%), Postives = 526/603 (87.23%), Query Frame = 0


Query: 1   MGYGRLNETDPGGTSGPGPAHDHLPTPNNPRKRRFLLLSLLSILLIVAAAVSATFLVGIR 60
           MG  RLNET P                 NPR+    +LSLLS++LI+  AVS T L+G+R
Sbjct: 1   MGCRRLNETHPAA---------------NPRRGPLSVLSLLSLILIL-PAVSPTSLLGLR 60

Query: 61  TASHGRSDPNLPLKPTQAISKACSRTRYPTLCVNSLLDFPDSLDADEHDLVHISFNITLQ 120
           T+    SDP LP KPT+AISKAC+R+R P LCV+SLLDFP SL A+E DLVHISFN+TL 
Sbjct: 61  TS----SDPKLPRKPTRAISKACARSRLPALCVDSLLDFPGSLSANERDLVHISFNLTLH 120

Query: 121 RLNKALYSSSEISPLQMDLRVRSAYDACLELLDDSVDALSRSLQSIAPSSSSSDGRPQRI 180
            LN+ALYSSS +S L MDL +RSAYDACLELLDDSV AL+RS  S+AP   SSD RP  +
Sbjct: 121 HLNEALYSSSHLSSLHMDLPLRSAYDACLELLDDSVHALTRSFHSLAP--PSSDSRPTPL 180

Query: 181 -GSSQDVITWLSAALTNQDTCTDGFAELTGAVKDQMTDKLHNLSELVSNCLALFSGSETS 240
             SS DVITWLSAALTNQDTCTDGF++L+G VKDQMTDKLHNLSELVSNCLALFSGS  +
Sbjct: 181 ASSSDDVITWLSAALTNQDTCTDGFSQLSGNVKDQMTDKLHNLSELVSNCLALFSGSVRN 240

Query: 241 DFDGVPIQNKRRLMEAADEE--SGNFPRWMSRRERRLLTMPVSALQADIVVSQNGSGTVK 300
           DF GVPIQNKRRLM AA++E  SGNFP WMSRRERRLLT+PV ALQADIVVS++G+GTVK
Sbjct: 241 DFSGVPIQNKRRLMNAAEKEDDSGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVK 300

Query: 301 TIAEAIKKAPQYSSRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGDKSIFD 360
           TIAEAIKKAP++S+R+TIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISG KSIFD
Sbjct: 301 TIAEAIKKAPEFSTRKTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGGKSIFD 360

Query: 361 NVTTFHTASFAATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDTLY 420
           NVTTFHTASFA +GAG+ILRDMTF NWAGP RHQAVALRV ADHAVVYRCN+IGYQDTLY
Sbjct: 361 NVTTFHTASFAVSGAGIILRDMTFVNWAGPARHQAVALRVSADHAVVYRCNVIGYQDTLY 420

Query: 421 VHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGI 480
           VHSNRQF+RECDIYGTVDFIFGNAAVVFQNCSIYARKAM+LQKNTITAQNRKDPNQNTGI
Sbjct: 421 VHSNRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAMELQKNTITAQNRKDPNQNTGI 480

Query: 481 SIHACRILATSDL-TASNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPHGWLEWNATFAL 540
           SIHACRILAT DL ++S ++NPTYLGRPWKLYSRVVYM SY+GGHVHP GWLEWNATFAL
Sbjct: 481 SIHACRILATPDLASSSTATNPTYLGRPWKLYSRVVYMQSYMGGHVHPRGWLEWNATFAL 540

Query: 541 DTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEANKFTVAQFIYGSSWLPSTGVAFLGG 600
           DTLYYGEYMNYGPGGAVGQRVTWPGYRVITS VEA++FTVAQFIYGSSWLPSTGVAFLGG
Sbjct: 541 DTLYYGEYMNYGPGGAVGQRVTWPGYRVITSRVEASQFTVAQFIYGSSWLPSTGVAFLGG 581

[Close]

BLAST of MS000492 vs. TAIR 10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 786.6 bits (2030), Expect = 1.4e-227
Identity = 408/612 (66.67%), Postives = 473/612 (77.29%), Query Frame = 0


Query: 1   MGYGRLNETDPGG------TSGP---GPAHDHLPTPNNPRKRRFLLLSLLSILLIVAAAV 60
           MGY RL  +   G      T+ P     +    P  NN R ++ L++S  SI+L ++  +
Sbjct: 1   MGYERLGPSGATGSVTTSTTTAPILNQVSTSEQPENNNRRSKKKLVVS--SIVLAISLIL 60

Query: 61  SATFLVGIRT-ASHGRSDPNLPLKPTQAISKACSRTRYPTLCVNSLLDFPDSLDA-DEHD 120
           +A    G+R+     +S P L  KP+QAISKAC  TR+P LCV+SL+DFP SL A    D
Sbjct: 61  AAAIFAGVRSRLKLNQSVPGLARKPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKD 120

Query: 121 LVHISFNITLQRLNKALYSSSEISPLQMDLRVRSAYDACLELLDDSVDALSRSLQSIAPS 180
           L+H++ N+TL   + ALYSS+ +S + M  R RSAYD+C+ELLDDSVDALSR+L S+  S
Sbjct: 121 LIHVTVNMTLHHFSHALYSSASLSFVDMPPRARSAYDSCVELLDDSVDALSRALSSVVSS 180

Query: 181 SSSSDGRPQRIGSSQDVITWLSAALTNQDTCTDGFAEL-TGAVKDQMTDKLHNLSELVSN 240
           S+            QDV TWLSAALTN DTCT+GF  +  G VKD MT  L NLSELVSN
Sbjct: 181 SA----------KPQDVTTWLSAALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSN 240

Query: 241 CLALFSGS-ETSDFDGVPIQNKRRLMEAADEESGNFPRWMSRRERRLLTMPVSALQADIV 300
           CLA+FS S +  DF GVPIQN+R L    +E    FPRWM  +ER +L MPVS +QADI+
Sbjct: 241 CLAIFSASHDGDDFAGVPIQNRRLL--GVEEREEKFPRWMRPKEREILEMPVSQIQADII 300

Query: 301 VSQNGSGTVKTIAEAIKKAPQYSSRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKT 360
           VS++G+GT KTI+EAIKKAPQ S+RR IIYV AGRYEE NLKVGRKK NLMFVGDGKGKT
Sbjct: 301 VSKDGNGTCKTISEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKT 360

Query: 361 VISGDKSIFDNVTTFHTASFAATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRC 420
           VISG KSIFDN+TTFHTASFAATGAG I RD+TFENWAGP +HQAVALR+GADHAV+YRC
Sbjct: 361 VISGGKSIFDNITTFHTASFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRC 420

Query: 421 NIIGYQDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQN 480
           NIIGYQDTLYVHSNRQF+RECDIYGTVDFIFGNAAVV QNCSIYARK MD QKNTITAQN
Sbjct: 421 NIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQN 480

Query: 481 RKDPNQNTGISIHACRILATSDLTASNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPHGW 540
           RKDPNQNTGISIHA R+LA SDL A+N S  TYLGRPWKL+SR VYM+SYIGGHVH  GW
Sbjct: 481 RKDPNQNTGISIHASRVLAASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGW 540

Query: 541 LEWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEANKFTVAQFIYGSSWLP 600
           LEWN TFALDTLYYGEY+N GPG  +GQRV+WPGYRVI ST EAN+FTVA+FIYGSSWLP
Sbjct: 541 LEWNTTFALDTLYYGEYLNSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLP 598

[Close]

BLAST of MS000492 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 765.8 bits (1976), Expect = 2.6e-221
Identity = 389/601 (64.73%), Postives = 463/601 (77.04%), Query Frame = 0


Query: 1   MGYGRLNETDPGGTSGPGPAHDHLPTPNNPRKRRFLLLSLLSILLIVAAAVSATFLVGIR 60
           MGY RL    P G S P         P   +K +  L+ L ++ ++V   V      GIR
Sbjct: 1   MGYDRLG---PSGPSNPNQKDPATSLPELQKKTKTKLI-LFTLAVLVVGVVCFGIFAGIR 60

Query: 61  TASHGRSDPNLPLKPTQAISKACSRTRYPTLCVNSLLDFPDSLDADEHDLVHISFNITLQ 120
               G+++P L  KPTQAIS+ CS++ YP LC+++LLDFP SL ADE++L+HISFN TLQ
Sbjct: 61  AVDSGKTEPKLTRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQ 120

Query: 121 RLNKALYSSSEISPLQMDLRVRSAYDACLELLDDSVDALSRSLQSIAPSSSSSDGRPQRI 180
           + +KALY+SS I+  QM  RVRSAYD+CLELLDDSVDAL+R+L S+   S          
Sbjct: 121 KFSKALYTSSTITYTQMPPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDE------- 180

Query: 181 GSSQDVITWLSAALTNQDTCTDGFAELT---GAVKDQMTDKLHNLSELVSNCLALFSGSE 240
            S  DV+TWLS+A+TN DTCTDGF E+    G VKDQ+   + +LSE+VSNCLA+F+G +
Sbjct: 181 -SHSDVMTWLSSAMTNHDTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAG-K 240

Query: 241 TSDFDGVPIQNKRRLMEAADEESGNFPRWMSRRERRLLTMPVSALQADIVVSQNGSGTVK 300
             D  GVP+ N R+L+    EE+   P W+ R +R LL  P SA+QADI VS++GSGT K
Sbjct: 241 VKDLSGVPVVNNRKLL--GTEETEELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFK 300

Query: 301 TIAEAIKKAPQYSSRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGDKSIFD 360
           TIAEAIKKAP++SSRR +IYV AGRYEE+NLKVGRKKTNLMF+GDGKGKTVI+G KSI D
Sbjct: 301 TIAEAIKKAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIAD 360

Query: 361 NVTTFHTASFAATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDTLY 420
           ++TTFHTA+FAATGAG I+RDMTFEN+AGP +HQAVALRVG DHAVVYRCNIIGYQD LY
Sbjct: 361 DLTTFHTATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALY 420

Query: 421 VHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGI 480
           VHSNRQF+REC+IYGTVDFIFGNAAV+ Q+C+IYARK M  QK TITAQNRKDPNQNTGI
Sbjct: 421 VHSNRQFFRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGI 480

Query: 481 SIHACRILATSDLTASNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPHGWLEWNATFALD 540
           SIHAC++LAT DL AS  S PTYLGRPWKLYSRVVYM+S +G H+ P GWLEWN  FALD
Sbjct: 481 SIHACKLLATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALD 540

Query: 541 TLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEANKFTVAQFIYGSSWLPSTGVAFLGGL 599
           +LYYGEYMN G G  +GQRV WPGY VITSTVEA+KFTVAQFI GSSWLPSTGV+F  GL
Sbjct: 541 SLYYGEYMNKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGL 586

[Close]

BLAST of MS000492 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 429.9 bits (1104), Expect = 3.4e-120
Identity = 240/580 (41.38%), Postives = 347/580 (59.83%), Query Frame = 0


Query: 44  LLIVAAAVSATFLVGIRTASHGRSDPN--LPLKPTQ--AISKACSRTRYPTLCVNSLLDF 103
           L++++AAV+  F+  +   S G S  N    L P+    +  +CS TRYP LC+++++  
Sbjct: 20  LVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCISAVVTA 79

Query: 104 PDSLDADEHDLVHISFNITLQRLNKALYSSSEISPLQMDL--RVRSAYDACLELLDDSVD 163
                  + D++  S N+T+  +    ++  ++   +  L  R ++A   CLE +D+++D
Sbjct: 80  GGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLETIDETLD 139

Query: 164 ALSRSLQSIAPSSSSSDGRPQRIGSSQDVITWLSAALTNQDTCTDGFA--ELTGAVKDQM 223
            L  +++ +    +    R      + D+ T +S+A+TNQ+TC DGF+  +    V+  +
Sbjct: 140 ELHETVEDLHLYPTKKTLREH----AGDLKTLISSAITNQETCLDGFSHDDADKQVRKAL 199

Query: 224 TDKLHNLSELVSNCLALFSGSETSDFDGVP-----IQNKRRLME-------------AAD 283
                ++  + SN LA+      +D            N R+L E             A +
Sbjct: 200 LKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAVDIAGAGE 259

Query: 284 EESGNFPRWMSRRERRLLTMPVSALQADIVVSQNGSGTVKTIAEAIKKAPQYSSRRTIIY 343
            +S  +P W+S  +RRLL    S ++AD  V+ +GSGT KT+A A+  AP+ S++R +I+
Sbjct: 260 LDSEGWPTWLSAGDRRLL--QGSGVKADATVAADGSGTFKTVAAAVAAAPENSNKRYVIH 319

Query: 344 VMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGDKSIFDNVTTFHTASFAATGAGLILR 403
           + AG Y E N++V +KK N+MF+GDG+ +T+I+G +++ D  TTFH+A+ AA G   + R
Sbjct: 320 IKAGVYRE-NVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAVGERFLAR 379

Query: 404 DMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDTLYVHSNRQFYRECDIYGTVDFI 463
           D+TF+N AGP +HQAVALRVG+D +  Y C+++ YQDTLYVHSNRQF+ +C I GTVDFI
Sbjct: 380 DITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLIAGTVDFI 439

Query: 464 FGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGISIHACRILATSDLTASNSSN 523
           FGNAAVV Q+C I+AR+    QKN +TAQ R DPNQNTGI I  CRI ATSDL +   S 
Sbjct: 440 FGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQSVKGSF 499

Query: 524 PTYLGRPWKLYSRVVYMLSYIGGHVHPHGWLEWNATFALDTLYYGEYMNYGPGGAVGQRV 583
           PTYLGRPWK YS+ V M S I   + P GW EW  TFAL+TL Y EY N G G     RV
Sbjct: 500 PTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAGAGTANRV 559

Query: 584 TWPGYRVITSTVEANKFTVAQFIYGSSWLPSTGVAFLGGL 598
            W G++VIT+  EA K+T  QFI G  WL STG  F  GL
Sbjct: 560 KWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

[Close]

BLAST of MS000492 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 422.9 bits (1086), Expect = 4.2e-118
Identity = 236/579 (40.76%), Postives = 340/579 (58.72%), Query Frame = 0


Query: 31  RKRRFLLLSLLSILLIVAAAVSATFLVGIRTASHGRSDPNLPLKPTQ--AISKACSRTRY 90
           +  + L+LS  +I L++ A++     +   T +  ++     L  T    +   CS T Y
Sbjct: 15  KNNKKLILSSAAIALLLLASIVG---IAATTTNQNKNQKITTLSSTSHAILKSVCSSTLY 74

Query: 91  PTLCVNSLLDFPDSLDADEHDLVHISFNITLQRLNKALYSSSEISPLQMDLRVR--SAYD 150
           P LC +++          + +++  S N+T + +    ++  ++   +  L  R  +A  
Sbjct: 75  PELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALH 134

Query: 151 ACLELLDDSVDALSRSLQSIAPSSSSSDGRPQRIGSSQDVITWLSAALTNQDTCTDGFA- 210
            CLE +D+++D L  +++ +         R      + D+ T +S+A+TNQ TC DGF+ 
Sbjct: 135 DCLETIDETLDELHVAVEDLHQYPKQKSLRKH----ADDLKTLISSAITNQGTCLDGFSY 194

Query: 211 -ELTGAVKDQMTDKLHNLSELVSNCLALFSGSETSDFDGVPIQ---------NKRRLME- 270
            +    V+  +     ++  + SN LA+      +D     ++         N R+L E 
Sbjct: 195 DDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEV 254

Query: 271 AADEESGNFPRWMSRRERRLLTMPVSALQADIVVSQNGSGTVKTIAEAIKKAPQYSSRRT 330
             D +S  +P+W+S  +RRLL    S ++AD  V+ +GSG   T+A A+  AP+ S++R 
Sbjct: 255 TGDLDSDGWPKWLSVGDRRLL--QGSTIKADATVADDGSGDFTTVAAAVAAAPEKSNKRF 314

Query: 331 IIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGDKSIFDNVTTFHTASFAATGAGL 390
           +I++ AG Y E N++V +KKTN+MF+GDG+GKT+I+G +++ D  TTFH+A+ AA G   
Sbjct: 315 VIHIKAGVYRE-NVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERF 374

Query: 391 ILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDTLYVHSNRQFYRECDIYGTV 450
           + RD+TF+N AGP +HQAVALRVG+D +  Y+C++  YQDTLYVHSNRQF+ +C I GTV
Sbjct: 375 LARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTV 434

Query: 451 DFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGISIHACRILATSDLTASN 510
           DFIFGNAA V Q+C I AR+    QKN +TAQ R DPNQNTGI I  CRI  TSDL A  
Sbjct: 435 DFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVK 494

Query: 511 SSNPTYLGRPWKLYSRVVYMLSYIGGHVHPHGWLEWNATFALDTLYYGEYMNYGPGGAVG 570
            + PTYLGRPWK YSR V M S I   + P GW EW+ +FALDTL Y EY+N G G    
Sbjct: 495 GTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTA 554

Query: 571 QRVTWPGYRVITSTVEANKFTVAQFIYGSSWLPSTGVAF 594
            RV W GY+VITS  EA  FT  QFI G  WL STG  F
Sbjct: 555 NRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPF 583

[Close]

BLAST of MS000492 vs. TAIR 10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 421.0 bits (1081), Expect = 1.6e-117
Identity = 223/477 (46.75%), Postives = 301/477 (63.10%), Query Frame = 0


Query: 122 LNKALYSSSEISPLQMDLRVRSAYDACLELLDDSVDALSRSLQSIAPSSSSSDGRPQRIG 181
           + KA+   S+        RV +A   C++LLD + + LS     I  +S S +G+    G
Sbjct: 58  ITKAVAIVSKFDKKAGKSRVSNAIVDCVDLLDSAAEELS----WIISASQSPNGKDNSTG 117

Query: 182 S-SQDVITWLSAALTNQDTCTDGFAELTGAVKDQMTDKLHNLSELVSNCLALFSGSETSD 241
               D+ TW+SAAL+NQDTC DGF    G +K  +   L  +   V N L +     +  
Sbjct: 118 DVGSDLRTWISAALSNQDTCLDGFEGTNGIIKKIVAGGLSKVGTTVRNLLTMVHSPPSKP 177

Query: 242 FDGVPIQNKRRLMEAADEESGNFPRWMSRRERRLLTMPVSALQADIVVSQNGSGTVKTIA 301
               P   K + M  A      FP W+   +R+LL    +   AD VV+ +G+G   TI+
Sbjct: 178 ---KPKPIKAQTMTKAHSGFSKFPSWVKPGDRKLLQTD-NITVADAVVAADGTGNFTTIS 237

Query: 302 EAIKKAPQYSSRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGDKSIFDNVT 361
           +A+  AP YS++R +I+V  G Y E N+++ +KK N+M VGDG   TVI+G++S  D  T
Sbjct: 238 DAVLAAPDYSTKRYVIHVKRGVYVE-NVEIKKKKWNIMMVGDGIDATVITGNRSFIDGWT 297

Query: 362 TFHTASFAATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDTLYVHS 421
           TF +A+FA +G G I RD+TF+N AGP +HQAVA+R   D  V YRC + GYQDTLY HS
Sbjct: 298 TFRSATFAVSGRGFIARDITFQNTAGPEKHQAVAIRSDTDLGVFYRCAMRGYQDTLYAHS 357

Query: 422 NRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGISIH 481
            RQF+REC I GTVDFIFG+A  VFQ+C I A++ +  QKN+ITAQ RKDPN+ TG +I 
Sbjct: 358 MRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQGLPNQKNSITAQGRKDPNEPTGFTIQ 417

Query: 482 ACRILATSDLTASNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPHGWLEWNATFALDTLY 541
              I A +DL  + ++  TYLGRPWKLYSR V+M +Y+   ++P GWLEWN  FALDTLY
Sbjct: 418 FSNIAADTDLLLNLNTTATYLGRPWKLYSRTVFMQNYMSDAINPVGWLEWNGNFALDTLY 477

Query: 542 YGEYMNYGPGGAVGQRVTWPGYRVITSTVEANKFTVAQFIYGSSWLPSTGVAFLGGL 598
           YGEYMN GPG ++ +RV WPGY V+ ++ EAN FTV+Q I G+ WLPSTG+ F+ GL
Sbjct: 478 YGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQLIQGNLWLPSTGITFIAGL 525

The following BLAST results are available for this feature:

BLAST of MS000492 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023553011.1	2.3e-307	89.20	probable pectinesterase/pectinesterase inhibitor 34 [Cucurbita pepo subsp. pepo]	[more]
XP_022922767.1	2.1e-305	88.70	probable pectinesterase/pectinesterase inhibitor 34 [Cucurbita moschata]	[more]
XP_038881267.1	1.0e-304	89.26	probable pectinesterase/pectinesterase inhibitor 34 [Benincasa hispida]	[more]
XP_004146219.1	5.2e-304	87.83	probable pectinesterase/pectinesterase inhibitor 34 [Cucumis sativus] >KGN57675....	[more]
XP_022984620.1	1.2e-303	88.21	probable pectinesterase/pectinesterase inhibitor 34 [Cucurbita maxima]	[more]

BLAST of MS000492 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M3B0	2.0e-226	66.67	Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3...	[more]
Q9FK05	3.7e-220	64.73	Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3...	[more]
P83948	1.3e-124	43.52	Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1	[more]
O04886	5.0e-124	43.70	Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1	[more]
Q43062	3.9e-121	49.35	Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=...	[more]

BLAST of MS000492 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E487	1.0e-305	88.70	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430666 PE=3 SV=1	[more]
A0A0A0L6S0	2.5e-304	87.83	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G239880 PE=3 SV=1	[more]
A0A6J1J930	5.6e-304	88.21	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111482853 PE=3 SV=1	[more]
A0A5A7U3N7	8.1e-303	87.50	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold175G00145...	[more]
A0A6J1G0F6	1.4e-267	79.44	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111449561 PE=3 SV=1	[more]

BLAST of MS000492 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G49220.1	1.4e-227	66.67	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G53370.1	2.6e-221	64.73	pectin methylesterase PCR fragment F	[more]
AT3G14310.1	3.4e-120	41.38	pectin methylesterase 3	[more]
AT1G53830.1	4.2e-118	40.76	pectin methylesterase 2	[more]
AT3G43270.1	1.6e-117	46.75	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 74..232 e-value: 2.7E-45 score: 166.5
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 53..233 e-value: 2.9E-38 score: 129.0
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 78..232 e-value: 1.4E-32 score: 113.1
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 75..235 e-value: 4.8E-37 score: 129.3
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 77..234
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 285..582 e-value: 1.9E-141 score: 470.0
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 279..597 e-value: 4.0E-116 score: 389.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..27
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 25..597
None	No IPR available	PANTHER	PTHR31707:SF222	PECTINESTERASE	coord: 25..597
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 82..234 e-value: 7.72828E-46 score: 157.215
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 430..439
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 280..595

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS000492.1	MS000492.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity