MS000448 (gene) Bitter gourd (TR) v1

Overview
NameMS000448
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionPentatricopeptide repeat-containing protein
Locationscaffold44: 1725837 .. 1727759 (+)
RNA-Seq ExpressionMS000448
SyntenyMS000448
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCTAAAGATGGAGATTTGGAATTCAATTTATCGAAAACTATTGATATTTGTTTTCCTTTGCTGGCTCAGGATGCTTACTTTGATTTACTCTTTTCCAGTCATATCCAAAAGGATAGAATCTGTTAAATTTTCCTGGAGTGCAAGCAGTACAGTGGTAAGCCTTATCGTCAATTGTTTTGCTGCTAGCTAGAACTGAACTCGTGAAGAGTTATACTTATCGTCGTTTATTTTACAGTGTCCTGTTTTCTGATAAATGGAAATTGGATATCTGGAGTCACAAGTTGAGAGGCAAATTCTGACCTTCCCTTTCCCTTTCTTACACAATACTTTCGACTTTTTTGCAGGTATGCGCTGCAAAAGGTCCACGGCCGAGATATCCTCGGGTTTGGAAAACCCGAAAGAGAATTGGAACCATATCCAAGGCAGCAAAGCTTGTTGATTGTGTAAGCTCTCTTGCCTTTCTCTTACCAATCCCATTCCCACCATCCACAAAAAGAAGGGAGCCCCTATGCACATTCAAATTGGAATTCAATGGCAGAAAATCTTTCATTTCATCTTGATTCGATGCGTTTAGAGACTTCCTGTAGAAGTATATCGAGTGGTTTTAGGTTTTTCAGAATTACTTTCTAACGACATAACCAGACATTTAGTGCTTTTAATTGACGAAAATAGTTTTTCACTGTTTCAAAAATCAACACTAAACTCACCTTCAATAATGCTATCTATTTTTTTGTTTCTTGGGATCGCTTGGTAGGTATGGTGCTATTTTTGAACTTAAGTCATTTGCAGGATCGAGCAGTTTATTCGTTATCATTTTAAAAGTATTTACTTTTCCATTTAGGTCAAGGGACTGTCTAACGTCAAAGAGGAAGTTTATGGGGCTCTTGATTCCTTCATAGCCTGGGAACTAGAGTTTCCTCTTATTACTGTAAAGAAGGCCCTGAAGACCTTAGAGATTCAAAGAGAATGGAAGAGGATAATCCAGGTACTTTTTTCCTATATGGGATTTCTTACATGCTTTTTGTTCTTTCTATTATGCCCTTTTGTCCTGAGAATGTCTCCGATCAAATTAATTGAGCAAATTGGGTGCATTGTTGTAGTTGACAAAATGGATGTTAAGTAAAGGCCAAGGAAGAACAATGGGAAGCTATTTCACATTACTAAATGCCTTAGCTGAAGATGGAAGACTCGATGAAGCCGAAGAGCTTTGGAACAAATTGTTTTCTCAGTATCTGGAGAGCATGCCTAGGATGTTCTTTCATAAAATGATATCCCTCTATTATGACCAGGGCATGCACGACAAGTTATTCGAGGTATTCAGTTCTCCGCTTATTGTTACTGAAGCAGTTTCAACTGAATCAGCAATAAAGCTCTAAGTTTTCCTTCCCACTCCTAATCGAGAAAAAAAAAGGGGTTAAAGGCTATGATACATGGTGCATAGCATATTTTTAGTTACGATGGGTTATTTTAAGTTGACTTATTTTGGATTCCATAGATACTGAAATTCAAATGGGACTGTCCATAGGTATTTGCTGACATGGAAGAACTTGGAGTTCAACCAAATACGGAAATTGTCACTATGATAGGAAATGTCTTCCAAGAGTTGGGTATGTTTGATAAATATGAAAAGCTGAAGAAGAAATATCCTCCACTAAAATGGGAGTATCGATACGTTAAAGGAAAGCGTGTAAGAATACGAGCGAAGTACCTGAACGAATATGGTAATTCCAACAATGGTTCAAGTGAGCTGGACCAAAAAGATGACAGTTCAATCAAACTACTGGAGGAAGCTGAAACAGTTTCTAAGGATTCTAGTCTTGAAGATGAGGAAATGAGGGAAGACCCAGATGAAATATTGGAAGATGAAAGTAAGTTGGAGGCACCCGATTTTGAATACAATTTCATGGGGTATGGGCGATTG

mRNA sequence

CTTCTAAAGATGGAGATTTGGAATTCAATTTATCGAAAACTATTGATATTTGTTTTCCTTTGCTGGCTCAGGATGCTTACTTTGATTTACTCTTTTCCAGTCATATCCAAAAGGATAGAATCTGTTAAATTTTCCTGGAGTGCAAGCAGTACAGTGGTATGCGCTGCAAAAGGTCCACGGCCGAGATATCCTCGGGTTTGGAAAACCCGAAAGAGAATTGGAACCATATCCAAGGCAGCAAAGCTTGTTGATTGTGTCAAGGGACTGTCTAACGTCAAAGAGGAAGTTTATGGGGCTCTTGATTCCTTCATAGCCTGGGAACTAGAGTTTCCTCTTATTACTGTAAAGAAGGCCCTGAAGACCTTAGAGATTCAAAGAGAATGGAAGAGGATAATCCAGTTGACAAAATGGATGTTAAGTAAAGGCCAAGGAAGAACAATGGGAAGCTATTTCACATTACTAAATGCCTTAGCTGAAGATGGAAGACTCGATGAAGCCGAAGAGCTTTGGAACAAATTGTTTTCTCAGTATCTGGAGAGCATGCCTAGGATGTTCTTTCATAAAATGATATCCCTCTATTATGACCAGGGCATGCACGACAAGTTATTCGAGGTATTTGCTGACATGGAAGAACTTGGAGTTCAACCAAATACGGAAATTGTCACTATGATAGGAAATGTCTTCCAAGAGTTGGGTATGTTTGATAAATATGAAAAGCTGAAGAAGAAATATCCTCCACTAAAATGGGAGTATCGATACGTTAAAGGAAAGCGTGTAAGAATACGAGCGAAGTACCTGAACGAATATGGTAATTCCAACAATGGTTCAAGTGAGCTGGACCAAAAAGATGACAGTTCAATCAAACTACTGGAGGAAGCTGAAACAGTTTCTAAGGATTCTAGTCTTGAAGATGAGGAAATGAGGGAAGACCCAGATGAAATATTGGAAGATGAAAGTAAGTTGGAGGCACCCGATTTTGAATACAATTTCATGGGGTATGGGCGATTG

Coding sequence (CDS)

CTTCTAAAGATGGAGATTTGGAATTCAATTTATCGAAAACTATTGATATTTGTTTTCCTTTGCTGGCTCAGGATGCTTACTTTGATTTACTCTTTTCCAGTCATATCCAAAAGGATAGAATCTGTTAAATTTTCCTGGAGTGCAAGCAGTACAGTGGTATGCGCTGCAAAAGGTCCACGGCCGAGATATCCTCGGGTTTGGAAAACCCGAAAGAGAATTGGAACCATATCCAAGGCAGCAAAGCTTGTTGATTGTGTCAAGGGACTGTCTAACGTCAAAGAGGAAGTTTATGGGGCTCTTGATTCCTTCATAGCCTGGGAACTAGAGTTTCCTCTTATTACTGTAAAGAAGGCCCTGAAGACCTTAGAGATTCAAAGAGAATGGAAGAGGATAATCCAGTTGACAAAATGGATGTTAAGTAAAGGCCAAGGAAGAACAATGGGAAGCTATTTCACATTACTAAATGCCTTAGCTGAAGATGGAAGACTCGATGAAGCCGAAGAGCTTTGGAACAAATTGTTTTCTCAGTATCTGGAGAGCATGCCTAGGATGTTCTTTCATAAAATGATATCCCTCTATTATGACCAGGGCATGCACGACAAGTTATTCGAGGTATTTGCTGACATGGAAGAACTTGGAGTTCAACCAAATACGGAAATTGTCACTATGATAGGAAATGTCTTCCAAGAGTTGGGTATGTTTGATAAATATGAAAAGCTGAAGAAGAAATATCCTCCACTAAAATGGGAGTATCGATACGTTAAAGGAAAGCGTGTAAGAATACGAGCGAAGTACCTGAACGAATATGGTAATTCCAACAATGGTTCAAGTGAGCTGGACCAAAAAGATGACAGTTCAATCAAACTACTGGAGGAAGCTGAAACAGTTTCTAAGGATTCTAGTCTTGAAGATGAGGAAATGAGGGAAGACCCAGATGAAATATTGGAAGATGAAAGTAAGTTGGAGGCACCCGATTTTGAATACAATTTCATGGGGTATGGGCGATTG

Protein sequence

LLKMEIWNSIYRKLLIFVFLCWLRMLTLIYSFPVISKRIESVKFSWSASSTVVCAAKGPRPRYPRVWKTRKRIGTISKAAKLVDCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALKTLEIQREWKRIIQLTKWMLSKGQGRTMGSYFTLLNALAEDGRLDEAEELWNKLFSQYLESMPRMFFHKMISLYYDQGMHDKLFEVFADMEELGVQPNTEIVTMIGNVFQELGMFDKYEKLKKKYPPLKWEYRYVKGKRVRIRAKYLNEYGNSNNGSSELDQKDDSSIKLLEEAETVSKDSSLEDEEMREDPDEILEDESKLEAPDFEYNFMGYGRL
Homology
BLAST of MS000448 vs. NCBI nr
Match: XP_022140817.1 (pentatricopeptide repeat-containing protein At4g21190 [Momordica charantia])

HSP 1 Score: 613.6 bits (1581), Expect = 1.0e-171
Identity = 312/313 (99.68%), Postives = 312/313 (99.68%), Query Frame = 0

Query: 24  RMLTLIYSFPVISKRIESVKFSWSASSTVVCAAKGPRPRYPRVWKTRKRIGTISKAAKLV 83
           RM TLIYSFPVISKRIESVKFSWSASSTVVCAAKGPRPRYPRVWKTRKRIGTISKAAKLV
Sbjct: 30  RMRTLIYSFPVISKRIESVKFSWSASSTVVCAAKGPRPRYPRVWKTRKRIGTISKAAKLV 89

Query: 84  DCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALKTLEIQREWKRIIQLTKWMLSKGQ 143
           DCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALKTLEIQREWKRIIQLTKWMLSKGQ
Sbjct: 90  DCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALKTLEIQREWKRIIQLTKWMLSKGQ 149

Query: 144 GRTMGSYFTLLNALAEDGRLDEAEELWNKLFSQYLESMPRMFFHKMISLYYDQGMHDKLF 203
           GRTMGSYFTLLNALAEDGRLDEAEELWNKLFSQYLESMPRMFFHKMISLYYDQGMHDKLF
Sbjct: 150 GRTMGSYFTLLNALAEDGRLDEAEELWNKLFSQYLESMPRMFFHKMISLYYDQGMHDKLF 209

Query: 204 EVFADMEELGVQPNTEIVTMIGNVFQELGMFDKYEKLKKKYPPLKWEYRYVKGKRVRIRA 263
           EVFADMEELGVQPNTEIVTMIGNVFQELGMFDKYEKLKKKYPPLKWEYRYVKGKRVRIRA
Sbjct: 210 EVFADMEELGVQPNTEIVTMIGNVFQELGMFDKYEKLKKKYPPLKWEYRYVKGKRVRIRA 269

Query: 264 KYLNEYGNSNNGSSELDQKDDSSIKLLEEAETVSKDSSLEDEEMREDPDEILEDESKLEA 323
           KYLNEYGNSNNGSSELDQKDDSSIKLLEEAETVSKDSSLEDEEMREDPDEILEDESKLEA
Sbjct: 270 KYLNEYGNSNNGSSELDQKDDSSIKLLEEAETVSKDSSLEDEEMREDPDEILEDESKLEA 329

Query: 324 PDFEYNFMGYGRL 337
           PDFEYNFMGYGRL
Sbjct: 330 PDFEYNFMGYGRL 342

BLAST of MS000448 vs. NCBI nr
Match: XP_016899030.1 (PREDICTED: pentatricopeptide repeat-containing protein At4g21190 isoform X1 [Cucumis melo])

HSP 1 Score: 528.5 bits (1360), Expect = 4.3e-146
Identity = 272/337 (80.71%), Postives = 297/337 (88.13%), Query Frame = 0

Query: 1   LLKMEIWNSIYRKLLIFVFLCWLRMLTLIYSFPVISKRIESVKFSWSASSTVVCAAKGPR 60
           LL++EI  SI+R LLIF  +CW RMLTL+Y+FPV SKRIESV FSW  SS+VVCAAKGPR
Sbjct: 18  LLELEILISIHRNLLIFDLICWFRMLTLVYTFPVTSKRIESVNFSWCPSSSVVCAAKGPR 77

Query: 61  PRYPRVWKTRKRIGTISKAAKLVDCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALK 120
           PRYPRVWKTRKRIGTISKAAKLVDCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALK
Sbjct: 78  PRYPRVWKTRKRIGTISKAAKLVDCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALK 137

Query: 121 TLEIQREWKRIIQLTKWMLSKGQGRTMGSYFTLLNALAEDGRLDEAEELWNKLFSQYLES 180
           TLE QREWKRIIQLTKWMLSKGQGRTMGSYFTLLNAL EDGRLDEAEELWNKLFSQYLES
Sbjct: 138 TLENQREWKRIIQLTKWMLSKGQGRTMGSYFTLLNALVEDGRLDEAEELWNKLFSQYLES 197

Query: 181 MPRMFFHKMISLYYDQGMHDKLFEVFADMEELGVQPNTEIVTMIGNVFQELGMFDKYEKL 240
           MPR+FFHKMISLYYD+ MHDKLFEVFADMEELGVQPN  IVT +GN+FQELGM DKYEKL
Sbjct: 198 MPRIFFHKMISLYYDRAMHDKLFEVFADMEELGVQPNMAIVTKVGNIFQELGMLDKYEKL 257

Query: 241 KKKYPPLKWEYRYVKGKRVRIRAKYLNEYGNSNNGSSELDQKDDSSIKLLEEAETVSKDS 300
            KKYPP KWEYRY+KGKRV+IR KYL+E GNS NG SE ++ + SS   L+EAE  S+DS
Sbjct: 258 MKKYPPPKWEYRYIKGKRVKIRTKYLSENGNSMNGLSEQNKMEHSSTNSLDEAEITSEDS 317

Query: 301 SLE-DEEMREDPDEILEDESKLEAPDFEYNFMGYGRL 337
           SLE DEE+ +DPDEILEDE      +FE++FMG G+L
Sbjct: 318 SLEDDEEIGKDPDEILEDEHMWSKSNFEHDFMGLGQL 354

BLAST of MS000448 vs. NCBI nr
Match: XP_038888232.1 (pentatricopeptide repeat-containing protein At4g21190 isoform X1 [Benincasa hispida])

HSP 1 Score: 512.3 bits (1318), Expect = 3.2e-141
Identity = 261/314 (83.12%), Postives = 286/314 (91.08%), Query Frame = 0

Query: 24  RMLTLIYSFPVISKRIESVKFSWSASSTVVCAAKGPRPRYPRVWKTRKRIGTISKAAKLV 83
           RMLTLIYSFPVISKRIESV FSW ASS+VVCAAKGPRPRYPRVWKT+KRIGT+SKAAKLV
Sbjct: 18  RMLTLIYSFPVISKRIESVNFSWCASSSVVCAAKGPRPRYPRVWKTKKRIGTVSKAAKLV 77

Query: 84  DCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALKTLEIQREWKRIIQLTKWMLSKGQ 143
           DCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKAL+TLE QREWKRIIQLTKWMLSKGQ
Sbjct: 78  DCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALQTLENQREWKRIIQLTKWMLSKGQ 137

Query: 144 GRTMGSYFTLLNALAEDGRLDEAEELWNKLFSQYLESMPRMFFHKMISLYYDQGMHDKLF 203
           GRTMGSYFTLLNALAEDGRLDEAEELWNKLFSQ+LESMPR+FFHKMISLYYD+ MHDKLF
Sbjct: 138 GRTMGSYFTLLNALAEDGRLDEAEELWNKLFSQHLESMPRIFFHKMISLYYDRAMHDKLF 197

Query: 204 EVFADMEELGVQPNTEIVTMIGNVFQELGMFDKYEKLKKKYPPLKWEYRYVKGKRVRIRA 263
           EVFADMEELGVQPN EIVTM+GNVF ELGM DKYEKL KKYPP KWEYRY+KGKRVRIR+
Sbjct: 198 EVFADMEELGVQPNMEIVTMVGNVFHELGMLDKYEKLMKKYPPPKWEYRYIKGKRVRIRS 257

Query: 264 KYLNEYGNSNNGSSELDQKDDSSIKLLEEAETVSKDSSLEDE-EMREDPDEILEDESKLE 323
           KYL E GN NN  S+ D+ + SS KLLEEAE  S+D++LED+ EM EDP+EI +DE   +
Sbjct: 258 KYLYENGNPNNSLSKHDKMEHSSTKLLEEAEITSEDTNLEDDVEMSEDPNEIRKDECMSK 317

Query: 324 APDFEYNFMGYGRL 337
             +FE++FMG+G+L
Sbjct: 318 EYNFEHDFMGFGQL 331

BLAST of MS000448 vs. NCBI nr
Match: XP_038888233.1 (pentatricopeptide repeat-containing protein At4g21190 isoform X2 [Benincasa hispida])

HSP 1 Score: 510.0 bits (1312), Expect = 1.6e-140
Identity = 260/313 (83.07%), Postives = 285/313 (91.05%), Query Frame = 0

Query: 25  MLTLIYSFPVISKRIESVKFSWSASSTVVCAAKGPRPRYPRVWKTRKRIGTISKAAKLVD 84
           MLTLIYSFPVISKRIESV FSW ASS+VVCAAKGPRPRYPRVWKT+KRIGT+SKAAKLVD
Sbjct: 1   MLTLIYSFPVISKRIESVNFSWCASSSVVCAAKGPRPRYPRVWKTKKRIGTVSKAAKLVD 60

Query: 85  CVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALKTLEIQREWKRIIQLTKWMLSKGQG 144
           CVKGLSNVKEEVYGALDSFIAWELEFPLITVKKAL+TLE QREWKRIIQLTKWMLSKGQG
Sbjct: 61  CVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALQTLENQREWKRIIQLTKWMLSKGQG 120

Query: 145 RTMGSYFTLLNALAEDGRLDEAEELWNKLFSQYLESMPRMFFHKMISLYYDQGMHDKLFE 204
           RTMGSYFTLLNALAEDGRLDEAEELWNKLFSQ+LESMPR+FFHKMISLYYD+ MHDKLFE
Sbjct: 121 RTMGSYFTLLNALAEDGRLDEAEELWNKLFSQHLESMPRIFFHKMISLYYDRAMHDKLFE 180

Query: 205 VFADMEELGVQPNTEIVTMIGNVFQELGMFDKYEKLKKKYPPLKWEYRYVKGKRVRIRAK 264
           VFADMEELGVQPN EIVTM+GNVF ELGM DKYEKL KKYPP KWEYRY+KGKRVRIR+K
Sbjct: 181 VFADMEELGVQPNMEIVTMVGNVFHELGMLDKYEKLMKKYPPPKWEYRYIKGKRVRIRSK 240

Query: 265 YLNEYGNSNNGSSELDQKDDSSIKLLEEAETVSKDSSLEDE-EMREDPDEILEDESKLEA 324
           YL E GN NN  S+ D+ + SS KLLEEAE  S+D++LED+ EM EDP+EI +DE   + 
Sbjct: 241 YLYENGNPNNSLSKHDKMEHSSTKLLEEAEITSEDTNLEDDVEMSEDPNEIRKDECMSKE 300

Query: 325 PDFEYNFMGYGRL 337
            +FE++FMG+G+L
Sbjct: 301 YNFEHDFMGFGQL 313

BLAST of MS000448 vs. NCBI nr
Match: XP_004140747.2 (pentatricopeptide repeat-containing protein At4g21190 [Cucumis sativus] >KGN57423.1 hypothetical protein Csa_010957 [Cucumis sativus])

HSP 1 Score: 510.0 bits (1312), Expect = 1.6e-140
Identity = 262/319 (82.13%), Postives = 284/319 (89.03%), Query Frame = 0

Query: 19  FLCWLRMLTLIYSFPVISKRIESVKFSWSASSTVVCAAKGPRPRYPRVWKTRKRIGTISK 78
           FL   RMLTL+Y+FPV SKRIESV FSW  SS+VVCAAKGPRPRYPRVWKT+KRIGTISK
Sbjct: 20  FLASYRMLTLVYTFPVTSKRIESVNFSWCPSSSVVCAAKGPRPRYPRVWKTKKRIGTISK 79

Query: 79  AAKLVDCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALKTLEIQREWKRIIQLTKWM 138
           AAKLVDCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALKTLE QREWKRIIQLTKWM
Sbjct: 80  AAKLVDCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALKTLENQREWKRIIQLTKWM 139

Query: 139 LSKGQGRTMGSYFTLLNALAEDGRLDEAEELWNKLFSQYLESMPRMFFHKMISLYYDQGM 198
           LSKGQGRTMGSYFTLLNALAEDGRLDEAEELWNKLFSQ+LES+PR+FFHKMISLYYDQ M
Sbjct: 140 LSKGQGRTMGSYFTLLNALAEDGRLDEAEELWNKLFSQHLESIPRIFFHKMISLYYDQAM 199

Query: 199 HDKLFEVFADMEELGVQPNTEIVTMIGNVFQELGMFDKYEKLKKKYPPLKWEYRYVKGKR 258
           HDKLFEVFADMEELGVQPN  IVT +GNVFQELGM DKY+KL KKYPP KWEYRY+KGKR
Sbjct: 200 HDKLFEVFADMEELGVQPNMAIVTKVGNVFQELGMLDKYKKLMKKYPPPKWEYRYIKGKR 259

Query: 259 VRIRAKYLNEYGNSNNGSSELDQKDDSSIKLLEEAETVSKDSSLE-DEEMREDPDEILED 318
           V+IRAKYL+E GNSNNG SE  + + SS   ++EAE  S+DSSLE DE+M EDPDEILED
Sbjct: 260 VKIRAKYLSENGNSNNGLSEHAKMEHSSTNSIDEAEITSEDSSLEDDEDMSEDPDEILED 319

Query: 319 ESKLEAPDFEYNFMGYGRL 337
           E      +FE++FMG G+L
Sbjct: 320 EHMWSKSNFEHDFMGLGQL 338

BLAST of MS000448 vs. ExPASy Swiss-Prot
Match: Q8LG95 (Pentatricopeptide repeat-containing protein At4g21190 OS=Arabidopsis thaliana OX=3702 GN=EMB1417 PE=2 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 1.5e-98
Identity = 192/305 (62.95%), Postives = 234/305 (76.72%), Query Frame = 0

Query: 25  MLTLIYSFP--VISKRIESVK-FSWSASSTVVCAAKGPRPRYPRVWKTRKRIGTISKAAK 84
           ML+L YS P  ++  R  S K F+   ++ VVCAA+GPRPR PRVWKTRKRIGTISKAAK
Sbjct: 1   MLSLRYSLPYLLLQTRESSTKLFTKKPNNVVVCAARGPRPRSPRVWKTRKRIGTISKAAK 60

Query: 85  LVDCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALKTLEIQREWKRIIQLTKWMLSK 144
           ++ C+KGLSNVKEEVYGALDSFIAWELEFPL+ VKKAL  LE ++EWK+IIQ+TKWMLSK
Sbjct: 61  MIACIKGLSNVKEEVYGALDSFIAWELEFPLVIVKKALVILEDEKEWKKIIQVTKWMLSK 120

Query: 145 GQGRTMGSYFTLLNALAEDGRLDEAEELWNKLFSQYLESMPRMFFHKMISLYYDQGMHDK 204
           GQGRTMG+YF+LLNALAED RLDEAEELWNKLF ++LE  PR FF+KMIS+YY + MH K
Sbjct: 121 GQGRTMGTYFSLLNALAEDNRLDEAEELWNKLFMEHLEGTPRKFFNKMISIYYKRDMHQK 180

Query: 205 LFEVFADMEELGVQPNTEIVTMIGNVFQELGMFDKYEKLKKKYPPLKWEYRYVKGKRVRI 264
           LFEVFADMEELGV+PN  IV+M+G VF +L M DKYEKL KKYPP +WE+RY+KG+RV++
Sbjct: 181 LFEVFADMEELGVKPNVAIVSMVGKVFVKLEMKDKYEKLMKKYPPPQWEFRYIKGRRVKV 240

Query: 265 RAKYLNEYGNSNNGSSELDQKDDSSIKLLEE-AETVSKDSSLEDEEMREDPDEILEDESK 324
           +AK LNE      G S  + K D+ I+  EE  E +S++   E E +     +I   E  
Sbjct: 241 KAKQLNELSEGEGGLSSDEDKIDNEIESEEEDGEDLSEEEEDEKELLGGSQGQITSREPS 300

Query: 325 LEAPD 326
           L+  D
Sbjct: 301 LDHLD 305

BLAST of MS000448 vs. ExPASy Swiss-Prot
Match: Q2V3H0 (Pentatricopeptide repeat-containing protein At4g18975, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g18975 PE=2 SV=2)

HSP 1 Score: 177.6 bits (449), Expect = 2.4e-43
Identity = 92/203 (45.32%), Postives = 129/203 (63.55%), Query Frame = 0

Query: 66  VWKTRKRIGTISKAAKLVDCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALKTLEIQ 125
           +WK     G+  KA  LV  + GL N KE VYGAL+ ++AWE+EFP+I   KAL+ L  +
Sbjct: 85  LWKKNDSAGSGQKALNLVRMLSGLPNEKEAVYGALNKWVAWEVEFPIIAAAKALQILRKR 144

Query: 126 REWKRIIQLTKWMLSKGQGRTMGSYFTLLNALAEDGRLDEAEELWNKLFSQYLESMPRMF 185
            +W R+IQL KWMLSKGQG TMG+Y  LL A   D R DEAE LWN +   +  S+PR  
Sbjct: 145 SQWHRVIQLAKWMLSKGQGATMGTYDILLLAFDMDERADEAESLWNMILHTHTRSIPRRL 204

Query: 186 FHKMISLYYDQGMHDKLFEVFADMEELGVQPNTEIVTMIGNVFQELGMFDKYEKLKKKYP 245
           F +MI+LY    +HDK+ EVFADMEEL V P+ +    +   F+EL   +  + + ++Y 
Sbjct: 205 FARMIALYAHHDLHDKVIEVFADMEELKVSPDEDSARRVARAFRELNQEENRKLILRRYL 264

Query: 246 PLKWEYRYVKGKRVRIRAKYLNE 269
             +++Y Y  G+RVR++ +Y +E
Sbjct: 265 S-EYKYIYFNGERVRVK-RYFSE 285

BLAST of MS000448 vs. ExPASy TrEMBL
Match: A0A6J1CI57 (pentatricopeptide repeat-containing protein At4g21190 OS=Momordica charantia OX=3673 GN=LOC111011395 PE=4 SV=1)

HSP 1 Score: 613.6 bits (1581), Expect = 4.9e-172
Identity = 312/313 (99.68%), Postives = 312/313 (99.68%), Query Frame = 0

Query: 24  RMLTLIYSFPVISKRIESVKFSWSASSTVVCAAKGPRPRYPRVWKTRKRIGTISKAAKLV 83
           RM TLIYSFPVISKRIESVKFSWSASSTVVCAAKGPRPRYPRVWKTRKRIGTISKAAKLV
Sbjct: 30  RMRTLIYSFPVISKRIESVKFSWSASSTVVCAAKGPRPRYPRVWKTRKRIGTISKAAKLV 89

Query: 84  DCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALKTLEIQREWKRIIQLTKWMLSKGQ 143
           DCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALKTLEIQREWKRIIQLTKWMLSKGQ
Sbjct: 90  DCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALKTLEIQREWKRIIQLTKWMLSKGQ 149

Query: 144 GRTMGSYFTLLNALAEDGRLDEAEELWNKLFSQYLESMPRMFFHKMISLYYDQGMHDKLF 203
           GRTMGSYFTLLNALAEDGRLDEAEELWNKLFSQYLESMPRMFFHKMISLYYDQGMHDKLF
Sbjct: 150 GRTMGSYFTLLNALAEDGRLDEAEELWNKLFSQYLESMPRMFFHKMISLYYDQGMHDKLF 209

Query: 204 EVFADMEELGVQPNTEIVTMIGNVFQELGMFDKYEKLKKKYPPLKWEYRYVKGKRVRIRA 263
           EVFADMEELGVQPNTEIVTMIGNVFQELGMFDKYEKLKKKYPPLKWEYRYVKGKRVRIRA
Sbjct: 210 EVFADMEELGVQPNTEIVTMIGNVFQELGMFDKYEKLKKKYPPLKWEYRYVKGKRVRIRA 269

Query: 264 KYLNEYGNSNNGSSELDQKDDSSIKLLEEAETVSKDSSLEDEEMREDPDEILEDESKLEA 323
           KYLNEYGNSNNGSSELDQKDDSSIKLLEEAETVSKDSSLEDEEMREDPDEILEDESKLEA
Sbjct: 270 KYLNEYGNSNNGSSELDQKDDSSIKLLEEAETVSKDSSLEDEEMREDPDEILEDESKLEA 329

Query: 324 PDFEYNFMGYGRL 337
           PDFEYNFMGYGRL
Sbjct: 330 PDFEYNFMGYGRL 342

BLAST of MS000448 vs. ExPASy TrEMBL
Match: A0A1S4DTK8 (pentatricopeptide repeat-containing protein At4g21190 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103484127 PE=4 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 2.1e-146
Identity = 272/337 (80.71%), Postives = 297/337 (88.13%), Query Frame = 0

Query: 1   LLKMEIWNSIYRKLLIFVFLCWLRMLTLIYSFPVISKRIESVKFSWSASSTVVCAAKGPR 60
           LL++EI  SI+R LLIF  +CW RMLTL+Y+FPV SKRIESV FSW  SS+VVCAAKGPR
Sbjct: 18  LLELEILISIHRNLLIFDLICWFRMLTLVYTFPVTSKRIESVNFSWCPSSSVVCAAKGPR 77

Query: 61  PRYPRVWKTRKRIGTISKAAKLVDCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALK 120
           PRYPRVWKTRKRIGTISKAAKLVDCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALK
Sbjct: 78  PRYPRVWKTRKRIGTISKAAKLVDCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALK 137

Query: 121 TLEIQREWKRIIQLTKWMLSKGQGRTMGSYFTLLNALAEDGRLDEAEELWNKLFSQYLES 180
           TLE QREWKRIIQLTKWMLSKGQGRTMGSYFTLLNAL EDGRLDEAEELWNKLFSQYLES
Sbjct: 138 TLENQREWKRIIQLTKWMLSKGQGRTMGSYFTLLNALVEDGRLDEAEELWNKLFSQYLES 197

Query: 181 MPRMFFHKMISLYYDQGMHDKLFEVFADMEELGVQPNTEIVTMIGNVFQELGMFDKYEKL 240
           MPR+FFHKMISLYYD+ MHDKLFEVFADMEELGVQPN  IVT +GN+FQELGM DKYEKL
Sbjct: 198 MPRIFFHKMISLYYDRAMHDKLFEVFADMEELGVQPNMAIVTKVGNIFQELGMLDKYEKL 257

Query: 241 KKKYPPLKWEYRYVKGKRVRIRAKYLNEYGNSNNGSSELDQKDDSSIKLLEEAETVSKDS 300
            KKYPP KWEYRY+KGKRV+IR KYL+E GNS NG SE ++ + SS   L+EAE  S+DS
Sbjct: 258 MKKYPPPKWEYRYIKGKRVKIRTKYLSENGNSMNGLSEQNKMEHSSTNSLDEAEITSEDS 317

Query: 301 SLE-DEEMREDPDEILEDESKLEAPDFEYNFMGYGRL 337
           SLE DEE+ +DPDEILEDE      +FE++FMG G+L
Sbjct: 318 SLEDDEEIGKDPDEILEDEHMWSKSNFEHDFMGLGQL 354

BLAST of MS000448 vs. ExPASy TrEMBL
Match: A0A0A0L6Q9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G184050 PE=4 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 7.6e-141
Identity = 262/319 (82.13%), Postives = 284/319 (89.03%), Query Frame = 0

Query: 19  FLCWLRMLTLIYSFPVISKRIESVKFSWSASSTVVCAAKGPRPRYPRVWKTRKRIGTISK 78
           FL   RMLTL+Y+FPV SKRIESV FSW  SS+VVCAAKGPRPRYPRVWKT+KRIGTISK
Sbjct: 20  FLASYRMLTLVYTFPVTSKRIESVNFSWCPSSSVVCAAKGPRPRYPRVWKTKKRIGTISK 79

Query: 79  AAKLVDCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALKTLEIQREWKRIIQLTKWM 138
           AAKLVDCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALKTLE QREWKRIIQLTKWM
Sbjct: 80  AAKLVDCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALKTLENQREWKRIIQLTKWM 139

Query: 139 LSKGQGRTMGSYFTLLNALAEDGRLDEAEELWNKLFSQYLESMPRMFFHKMISLYYDQGM 198
           LSKGQGRTMGSYFTLLNALAEDGRLDEAEELWNKLFSQ+LES+PR+FFHKMISLYYDQ M
Sbjct: 140 LSKGQGRTMGSYFTLLNALAEDGRLDEAEELWNKLFSQHLESIPRIFFHKMISLYYDQAM 199

Query: 199 HDKLFEVFADMEELGVQPNTEIVTMIGNVFQELGMFDKYEKLKKKYPPLKWEYRYVKGKR 258
           HDKLFEVFADMEELGVQPN  IVT +GNVFQELGM DKY+KL KKYPP KWEYRY+KGKR
Sbjct: 200 HDKLFEVFADMEELGVQPNMAIVTKVGNVFQELGMLDKYKKLMKKYPPPKWEYRYIKGKR 259

Query: 259 VRIRAKYLNEYGNSNNGSSELDQKDDSSIKLLEEAETVSKDSSLE-DEEMREDPDEILED 318
           V+IRAKYL+E GNSNNG SE  + + SS   ++EAE  S+DSSLE DE+M EDPDEILED
Sbjct: 260 VKIRAKYLSENGNSNNGLSEHAKMEHSSTNSIDEAEITSEDSSLEDDEDMSEDPDEILED 319

Query: 319 ESKLEAPDFEYNFMGYGRL 337
           E      +FE++FMG G+L
Sbjct: 320 EHMWSKSNFEHDFMGLGQL 338

BLAST of MS000448 vs. ExPASy TrEMBL
Match: A0A6J1G1Y3 (pentatricopeptide repeat-containing protein At4g21190 OS=Cucurbita moschata OX=3662 GN=LOC111449934 PE=4 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 2.2e-140
Identity = 264/315 (83.81%), Postives = 288/315 (91.43%), Query Frame = 0

Query: 24  RMLTLIYSFPVISKRIESVKFSWSASSTVVCAAKGPRPRYPRVWKTRKRIGTISKAAKLV 83
           RM TLIYSFPVISK IESVKFS  ASS+VVCAAKGPRPRYPRVWKTRKRIGTISKAAKLV
Sbjct: 18  RMHTLIYSFPVISKGIESVKFSLIASSSVVCAAKGPRPRYPRVWKTRKRIGTISKAAKLV 77

Query: 84  DCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALKTLEIQREWKRIIQLTKWMLSKGQ 143
           DCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALKTLEIQREWKRIIQLTKWMLSKGQ
Sbjct: 78  DCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALKTLEIQREWKRIIQLTKWMLSKGQ 137

Query: 144 GRTMGSYFTLLNALAEDGRLDEAEELWNKLFSQYLESMPRMFFHKMISLYYDQGMHDKLF 203
           GRTMGSYFTLLNALA DGRLDEAEELWNKLFSQ+LESMPR+FFHKMISLYY++ MHDKLF
Sbjct: 138 GRTMGSYFTLLNALAADGRLDEAEELWNKLFSQHLESMPRIFFHKMISLYYERDMHDKLF 197

Query: 204 EVFADMEELGVQPNTEIVTMIGNVFQELGMFDKYEKLKKKYPPLKWEYRYVKGKRVRIRA 263
           E+FADMEELGVQP+  IVT +GNVFQ+LGM DKYEKLKKKYPP KWEYRY++GKRV+IRA
Sbjct: 198 EIFADMEELGVQPSMAIVTKLGNVFQKLGMLDKYEKLKKKYPPPKWEYRYIRGKRVKIRA 257

Query: 264 KYLNEYGNSNNGSSELDQKDDSSI-KLLEEAETVSKDSSLEDEEMREDPDEILEDES-KL 323
           K L+E G+SNNGS ELD+K+ SS  +LLEE E  S+DSSLED+EM EDPDE LEDES  L
Sbjct: 258 KNLHENGSSNNGSGELDKKEHSSTEELLEEDEITSQDSSLEDDEMSEDPDEALEDESMML 317

Query: 324 EAPDFEYNFMGYGRL 337
           +   FE++FMG+G+L
Sbjct: 318 KESTFEHDFMGFGQL 332

BLAST of MS000448 vs. ExPASy TrEMBL
Match: A0A5D3DHA7 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold386G001120 PE=4 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 2.9e-140
Identity = 259/314 (82.48%), Postives = 280/314 (89.17%), Query Frame = 0

Query: 24  RMLTLIYSFPVISKRIESVKFSWSASSTVVCAAKGPRPRYPRVWKTRKRIGTISKAAKLV 83
           RMLTL+Y+FPV SKRIESV FSW  SS+VVCAAKGPRPRYPRVWKTRKRIGTISKAAKLV
Sbjct: 18  RMLTLVYTFPVTSKRIESVNFSWCPSSSVVCAAKGPRPRYPRVWKTRKRIGTISKAAKLV 77

Query: 84  DCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALKTLEIQREWKRIIQLTKWMLSKGQ 143
           DCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALKTLE QREWKRIIQLTKWMLSKGQ
Sbjct: 78  DCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALKTLENQREWKRIIQLTKWMLSKGQ 137

Query: 144 GRTMGSYFTLLNALAEDGRLDEAEELWNKLFSQYLESMPRMFFHKMISLYYDQGMHDKLF 203
           GRTMGSYFTLLNAL EDGRLDEAEELWNKLFSQYLESMPR+FFHKMISLYYD+ MHDKLF
Sbjct: 138 GRTMGSYFTLLNALVEDGRLDEAEELWNKLFSQYLESMPRIFFHKMISLYYDRAMHDKLF 197

Query: 204 EVFADMEELGVQPNTEIVTMIGNVFQELGMFDKYEKLKKKYPPLKWEYRYVKGKRVRIRA 263
           EVFADMEELGVQPN  IVT +GN+FQELGM DKYEKL KKYPP KWEYRY+KGKRV+IR 
Sbjct: 198 EVFADMEELGVQPNMAIVTKVGNIFQELGMLDKYEKLMKKYPPPKWEYRYIKGKRVKIRT 257

Query: 264 KYLNEYGNSNNGSSELDQKDDSSIKLLEEAETVSKDSSLE-DEEMREDPDEILEDESKLE 323
           KYL+E GNS NG SE ++ + SS   L+EAE  S+DSSLE DEE+ +DPDEILEDE    
Sbjct: 258 KYLSENGNSKNGLSEQNKMEHSSTNSLDEAEITSEDSSLEDDEEIGKDPDEILEDEHMWS 317

Query: 324 APDFEYNFMGYGRL 337
             +FE++FMG G+L
Sbjct: 318 KSNFEHDFMGLGQL 331

BLAST of MS000448 vs. TAIR 10
Match: AT4G21190.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 360.9 bits (925), Expect = 1.1e-99
Identity = 192/305 (62.95%), Postives = 234/305 (76.72%), Query Frame = 0

Query: 25  MLTLIYSFP--VISKRIESVK-FSWSASSTVVCAAKGPRPRYPRVWKTRKRIGTISKAAK 84
           ML+L YS P  ++  R  S K F+   ++ VVCAA+GPRPR PRVWKTRKRIGTISKAAK
Sbjct: 1   MLSLRYSLPYLLLQTRESSTKLFTKKPNNVVVCAARGPRPRSPRVWKTRKRIGTISKAAK 60

Query: 85  LVDCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALKTLEIQREWKRIIQLTKWMLSK 144
           ++ C+KGLSNVKEEVYGALDSFIAWELEFPL+ VKKAL  LE ++EWK+IIQ+TKWMLSK
Sbjct: 61  MIACIKGLSNVKEEVYGALDSFIAWELEFPLVIVKKALVILEDEKEWKKIIQVTKWMLSK 120

Query: 145 GQGRTMGSYFTLLNALAEDGRLDEAEELWNKLFSQYLESMPRMFFHKMISLYYDQGMHDK 204
           GQGRTMG+YF+LLNALAED RLDEAEELWNKLF ++LE  PR FF+KMIS+YY + MH K
Sbjct: 121 GQGRTMGTYFSLLNALAEDNRLDEAEELWNKLFMEHLEGTPRKFFNKMISIYYKRDMHQK 180

Query: 205 LFEVFADMEELGVQPNTEIVTMIGNVFQELGMFDKYEKLKKKYPPLKWEYRYVKGKRVRI 264
           LFEVFADMEELGV+PN  IV+M+G VF +L M DKYEKL KKYPP +WE+RY+KG+RV++
Sbjct: 181 LFEVFADMEELGVKPNVAIVSMVGKVFVKLEMKDKYEKLMKKYPPPQWEFRYIKGRRVKV 240

Query: 265 RAKYLNEYGNSNNGSSELDQKDDSSIKLLEE-AETVSKDSSLEDEEMREDPDEILEDESK 324
           +AK LNE      G S  + K D+ I+  EE  E +S++   E E +     +I   E  
Sbjct: 241 KAKQLNELSEGEGGLSSDEDKIDNEIESEEEDGEDLSEEEEDEKELLGGSQGQITSREPS 300

Query: 325 LEAPD 326
           L+  D
Sbjct: 301 LDHLD 305

BLAST of MS000448 vs. TAIR 10
Match: AT4G18975.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 177.6 bits (449), Expect = 1.7e-44
Identity = 92/203 (45.32%), Postives = 129/203 (63.55%), Query Frame = 0

Query: 66  VWKTRKRIGTISKAAKLVDCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALKTLEIQ 125
           +WK     G+  KA  LV  + GL N KE VYGAL+ ++AWE+EFP+I   KAL+ L  +
Sbjct: 85  LWKKNDSAGSGQKALNLVRMLSGLPNEKEAVYGALNKWVAWEVEFPIIAAAKALQILRKR 144

Query: 126 REWKRIIQLTKWMLSKGQGRTMGSYFTLLNALAEDGRLDEAEELWNKLFSQYLESMPRMF 185
            +W R+IQL KWMLSKGQG TMG+Y  LL A   D R DEAE LWN +   +  S+PR  
Sbjct: 145 SQWHRVIQLAKWMLSKGQGATMGTYDILLLAFDMDERADEAESLWNMILHTHTRSIPRRL 204

Query: 186 FHKMISLYYDQGMHDKLFEVFADMEELGVQPNTEIVTMIGNVFQELGMFDKYEKLKKKYP 245
           F +MI+LY    +HDK+ EVFADMEEL V P+ +    +   F+EL   +  + + ++Y 
Sbjct: 205 FARMIALYAHHDLHDKVIEVFADMEELKVSPDEDSARRVARAFRELNQEENRKLILRRYL 264

Query: 246 PLKWEYRYVKGKRVRIRAKYLNE 269
             +++Y Y  G+RVR++ +Y +E
Sbjct: 265 S-EYKYIYFNGERVRVK-RYFSE 285

BLAST of MS000448 vs. TAIR 10
Match: AT4G18975.2 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 177.6 bits (449), Expect = 1.7e-44
Identity = 92/203 (45.32%), Postives = 129/203 (63.55%), Query Frame = 0

Query: 66  VWKTRKRIGTISKAAKLVDCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALKTLEIQ 125
           +WK     G+  KA  LV  + GL N KE VYGAL+ ++AWE+EFP+I   KAL+ L  +
Sbjct: 58  LWKKNDSAGSGQKALNLVRMLSGLPNEKEAVYGALNKWVAWEVEFPIIAAAKALQILRKR 117

Query: 126 REWKRIIQLTKWMLSKGQGRTMGSYFTLLNALAEDGRLDEAEELWNKLFSQYLESMPRMF 185
            +W R+IQL KWMLSKGQG TMG+Y  LL A   D R DEAE LWN +   +  S+PR  
Sbjct: 118 SQWHRVIQLAKWMLSKGQGATMGTYDILLLAFDMDERADEAESLWNMILHTHTRSIPRRL 177

Query: 186 FHKMISLYYDQGMHDKLFEVFADMEELGVQPNTEIVTMIGNVFQELGMFDKYEKLKKKYP 245
           F +MI+LY    +HDK+ EVFADMEEL V P+ +    +   F+EL   +  + + ++Y 
Sbjct: 178 FARMIALYAHHDLHDKVIEVFADMEELKVSPDEDSARRVARAFRELNQEENRKLILRRYL 237

Query: 246 PLKWEYRYVKGKRVRIRAKYLNE 269
             +++Y Y  G+RVR++ +Y +E
Sbjct: 238 S-EYKYIYFNGERVRVK-RYFSE 258

BLAST of MS000448 vs. TAIR 10
Match: AT4G18975.3 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 177.6 bits (449), Expect = 1.7e-44
Identity = 92/203 (45.32%), Postives = 129/203 (63.55%), Query Frame = 0

Query: 66  VWKTRKRIGTISKAAKLVDCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALKTLEIQ 125
           +WK     G+  KA  LV  + GL N KE VYGAL+ ++AWE+EFP+I   KAL+ L  +
Sbjct: 85  LWKKNDSAGSGQKALNLVRMLSGLPNEKEAVYGALNKWVAWEVEFPIIAAAKALQILRKR 144

Query: 126 REWKRIIQLTKWMLSKGQGRTMGSYFTLLNALAEDGRLDEAEELWNKLFSQYLESMPRMF 185
            +W R+IQL KWMLSKGQG TMG+Y  LL A   D R DEAE LWN +   +  S+PR  
Sbjct: 145 SQWHRVIQLAKWMLSKGQGATMGTYDILLLAFDMDERADEAESLWNMILHTHTRSIPRRL 204

Query: 186 FHKMISLYYDQGMHDKLFEVFADMEELGVQPNTEIVTMIGNVFQELGMFDKYEKLKKKYP 245
           F +MI+LY    +HDK+ EVFADMEEL V P+ +    +   F+EL   +  + + ++Y 
Sbjct: 205 FARMIALYAHHDLHDKVIEVFADMEELKVSPDEDSARRVARAFRELNQEENRKLILRRYL 264

Query: 246 PLKWEYRYVKGKRVRIRAKYLNE 269
             +++Y Y  G+RVR++ +Y +E
Sbjct: 265 S-EYKYIYFNGERVRVK-RYFSE 285

BLAST of MS000448 vs. TAIR 10
Match: AT4G18975.4 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 177.6 bits (449), Expect = 1.7e-44
Identity = 92/203 (45.32%), Postives = 129/203 (63.55%), Query Frame = 0

Query: 66  VWKTRKRIGTISKAAKLVDCVKGLSNVKEEVYGALDSFIAWELEFPLITVKKALKTLEIQ 125
           +WK     G+  KA  LV  + GL N KE VYGAL+ ++AWE+EFP+I   KAL+ L  +
Sbjct: 85  LWKKNDSAGSGQKALNLVRMLSGLPNEKEAVYGALNKWVAWEVEFPIIAAAKALQILRKR 144

Query: 126 REWKRIIQLTKWMLSKGQGRTMGSYFTLLNALAEDGRLDEAEELWNKLFSQYLESMPRMF 185
            +W R+IQL KWMLSKGQG TMG+Y  LL A   D R DEAE LWN +   +  S+PR  
Sbjct: 145 SQWHRVIQLAKWMLSKGQGATMGTYDILLLAFDMDERADEAESLWNMILHTHTRSIPRRL 204

Query: 186 FHKMISLYYDQGMHDKLFEVFADMEELGVQPNTEIVTMIGNVFQELGMFDKYEKLKKKYP 245
           F +MI+LY    +HDK+ EVFADMEEL V P+ +    +   F+EL   +  + + ++Y 
Sbjct: 205 FARMIALYAHHDLHDKVIEVFADMEELKVSPDEDSARRVARAFRELNQEENRKLILRRYL 264

Query: 246 PLKWEYRYVKGKRVRIRAKYLNE 269
             +++Y Y  G+RVR++ +Y +E
Sbjct: 265 S-EYKYIYFNGERVRVK-RYFSE 285

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022140817.11.0e-17199.68pentatricopeptide repeat-containing protein At4g21190 [Momordica charantia][more]
XP_016899030.14.3e-14680.71PREDICTED: pentatricopeptide repeat-containing protein At4g21190 isoform X1 [Cuc... [more]
XP_038888232.13.2e-14183.12pentatricopeptide repeat-containing protein At4g21190 isoform X1 [Benincasa hisp... [more]
XP_038888233.11.6e-14083.07pentatricopeptide repeat-containing protein At4g21190 isoform X2 [Benincasa hisp... [more]
XP_004140747.21.6e-14082.13pentatricopeptide repeat-containing protein At4g21190 [Cucumis sativus] >KGN5742... [more]
Match NameE-valueIdentityDescription
Q8LG951.5e-9862.95Pentatricopeptide repeat-containing protein At4g21190 OS=Arabidopsis thaliana OX... [more]
Q2V3H02.4e-4345.32Pentatricopeptide repeat-containing protein At4g18975, chloroplastic OS=Arabidop... [more]
Match NameE-valueIdentityDescription
A0A6J1CI574.9e-17299.68pentatricopeptide repeat-containing protein At4g21190 OS=Momordica charantia OX=... [more]
A0A1S4DTK82.1e-14680.71pentatricopeptide repeat-containing protein At4g21190 isoform X1 OS=Cucumis melo... [more]
A0A0A0L6Q97.6e-14182.13Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G184050 PE=4 SV=1[more]
A0A6J1G1Y32.2e-14083.81pentatricopeptide repeat-containing protein At4g21190 OS=Cucurbita moschata OX=3... [more]
A0A5D3DHA72.9e-14082.48Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946... [more]
Match NameE-valueIdentityDescription
AT4G21190.11.1e-9962.95Pentatricopeptide repeat (PPR) superfamily protein [more]
AT4G18975.11.7e-4445.32Pentatricopeptide repeat (PPR) superfamily protein [more]
AT4G18975.21.7e-4445.32Pentatricopeptide repeat (PPR) superfamily protein [more]
AT4G18975.31.7e-4445.32Pentatricopeptide repeat (PPR) superfamily protein [more]
AT4G18975.41.7e-4445.32Pentatricopeptide repeat (PPR) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 152..173
e-value: 0.023
score: 14.9
coord: 189..214
e-value: 0.053
score: 13.8
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 186..218
e-value: 2.1E-4
score: 19.3
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 146..180
score: 8.549871
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 182..216
score: 8.867749
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 77..245
e-value: 9.6E-16
score: 59.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 305..319
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 293..319
NoneNo IPR availablePANTHERPTHR46782:SF1OS01G0757700 PROTEINcoord: 26..308
IPR044646Pentatricopeptide repeat-containing protein EMB1417-likePANTHERPTHR46782OS01G0757700 PROTEINcoord: 26..308

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS000448.1MS000448.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding