MS000425 (gene) Bitter gourd (TR) v1

Overview


Name	MS000425
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	rRNA 2'-O-methyltransferase fibrillarin 2
Location	scaffold44: 1619255 .. 1619434 (+)
RNA-Seq Expression	MS000425
Synteny	MS000425

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTCAAGCTAACTGCATTGATTCAACAGTTCCAGCAGAGACAGTGTTTGCAAGTGAGGTGAATAAGTTAAAGGCAGATCAATTCAAACCCATCGAGCGAGTAACTCTTGAACCATTTGAACGAGATCATGCTTGTGTTGTCGGTATCTATCAGGCGCCAAAGAAACAGAAGCTCGCTGCA

mRNA sequence

TTTCAAGCTAACTGCATTGATTCAACAGTTCCAGCAGAGACAGTGTTTGCAAGTGAGGTGAATAAGTTAAAGGCAGATCAATTCAAACCCATCGAGCGAGTAACTCTTGAACCATTTGAACGAGATCATGCTTGTGTTGTCGGTATCTATCAGGCGCCAAAGAAACAGAAGCTCGCTGCA

Coding sequence (CDS)

TTTCAAGCTAACTGCATTGATTCAACAGTTCCAGCAGAGACAGTGTTTGCAAGTGAGGTGAATAAGTTAAAGGCAGATCAATTCAAACCCATCGAGCGAGTAACTCTTGAACCATTTGAACGAGATCATGCTTGTGTTGTCGGTATCTATCAGGCGCCAAAGAAACAGAAGCTCGCTGCA

Protein sequence

FQANCIDSTVPAETVFASEVNKLKADQFKPIERVTLEPFERDHACVVGIYQAPKKQKLAA

Homology

[Close]

BLAST of MS000425 vs. NCBI nr
Match: KGN57431.2 (hypothetical protein Csa_009607 [Cucumis sativus])

HSP 1 Score: 111.7 bits (278), Expect = 2.2e-21
Identity = 53/59 (89.83%), Postives = 56/59 (94.92%), Query Frame = 0


Query: 2   QANCIDSTVPAETVFASEVNKLKADQFKPIERVTLEPFERDHACVVGIYQAPKKQKLAA 61
           +ANCIDSTVPAE VFASEVNKLKADQFKP E+VTLEPFERDHACVVGIY+APKKQK AA
Sbjct: 226 KANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKAAA 284

[Close]

BLAST of MS000425 vs. NCBI nr
Match: XP_022922917.1 (probable mediator of RNA polymerase II transcription subunit 36b [Cucurbita moschata])

HSP 1 Score: 111.7 bits (278), Expect = 2.2e-21
Identity = 53/59 (89.83%), Postives = 56/59 (94.92%), Query Frame = 0


Query: 2   QANCIDSTVPAETVFASEVNKLKADQFKPIERVTLEPFERDHACVVGIYQAPKKQKLAA 61
           +ANCIDSTVPAE VFASEVNKLKADQFKP E+VTLEPFERDHACVVGIY+APKKQK AA
Sbjct: 246 KANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKAAA 304

[Close]

BLAST of MS000425 vs. NCBI nr
Match: XP_023552409.1 (mediator of RNA polymerase II transcription subunit 36a-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 111.7 bits (278), Expect = 2.2e-21
Identity = 53/59 (89.83%), Postives = 56/59 (94.92%), Query Frame = 0


Query: 2   QANCIDSTVPAETVFASEVNKLKADQFKPIERVTLEPFERDHACVVGIYQAPKKQKLAA 61
           +ANCIDSTVPAE VFASEVNKLKADQFKP E+VTLEPFERDHACVVGIY+APKKQK AA
Sbjct: 247 KANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKAAA 305

[Close]

BLAST of MS000425 vs. NCBI nr
Match: XP_004143621.2 (probable mediator of RNA polymerase II transcription subunit 36b [Cucumis sativus] >KGN50438.1 hypothetical protein Csa_000027 [Cucumis sativus])

HSP 1 Score: 111.7 bits (278), Expect = 2.2e-21
Identity = 53/59 (89.83%), Postives = 56/59 (94.92%), Query Frame = 0


Query: 2   QANCIDSTVPAETVFASEVNKLKADQFKPIERVTLEPFERDHACVVGIYQAPKKQKLAA 61
           +ANCIDSTVPAE VFASEVNKLKADQFKP E+VTLEPFERDHACVVGIY+APKKQK AA
Sbjct: 249 KANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKAAA 307

[Close]

BLAST of MS000425 vs. NCBI nr
Match: XP_038875959.1 (rRNA 2'-O-methyltransferase fibrillarin 1-like [Benincasa hispida])

HSP 1 Score: 111.7 bits (278), Expect = 2.2e-21
Identity = 53/59 (89.83%), Postives = 56/59 (94.92%), Query Frame = 0


Query: 2   QANCIDSTVPAETVFASEVNKLKADQFKPIERVTLEPFERDHACVVGIYQAPKKQKLAA 61
           +ANCIDSTVPAE VFASEVNKLKADQFKP E+VTLEPFERDHACVVGIY+APKKQK AA
Sbjct: 247 KANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKAAA 305

[Close]

BLAST of MS000425 vs. ExPASy Swiss-Prot
Match: Q9FEF8 (rRNA 2'-O-methyltransferase fibrillarin 1 OS=Arabidopsis thaliana OX=3702 GN=FIB1 PE=1 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 1.8e-18
Identity = 44/59 (74.58%), Postives = 49/59 (83.05%), Query Frame = 0


Query: 2   QANCIDSTVPAETVFASEVNKLKADQFKPIERVTLEPFERDHACVVGIYQAPKKQKLAA 61
           +ANCIDSTV AE VF SEV KL+ +QFKP E+VTLEPFERDHACVVG Y+ PKKQK  A
Sbjct: 249 KANCIDSTVAAEAVFQSEVKKLQQEQFKPAEQVTLEPFERDHACVVGGYRMPKKQKTPA 307

[Close]

BLAST of MS000425 vs. ExPASy Swiss-Prot
Match: Q94AH9 (rRNA 2'-O-methyltransferase fibrillarin 2 OS=Arabidopsis thaliana OX=3702 GN=FIB2 PE=1 SV=2)

HSP 1 Score: 92.4 bits (228), Expect = 1.8e-18
Identity = 43/58 (74.14%), Postives = 49/58 (84.48%), Query Frame = 0


Query: 2   QANCIDSTVPAETVFASEVNKLKADQFKPIERVTLEPFERDHACVVGIYQAPKKQKLA 60
           +ANCIDSTVPAE VF +EV KL+ +QFKP E+VTLEPFERDHACVVG Y+ PKK K A
Sbjct: 260 KANCIDSTVPAEAVFQTEVKKLQQEQFKPAEQVTLEPFERDHACVVGGYRMPKKPKAA 317

[Close]

BLAST of MS000425 vs. ExPASy Swiss-Prot
Match: P35549 (rRNA 2'-O-methyltransferase fibrillarin OS=Leishmania major OX=5664 PE=3 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 5.5e-15
Identity = 37/56 (66.07%), Postives = 46/56 (82.14%), Query Frame = 0


Query: 2   QANCIDSTVPAETVFASEVNKLKADQFKPIERVTLEPFERDHACVVGIYQAPKKQK 58
           +ANCIDST+ A  V ASE+NKLK D+ KP+E+V+LEPFERDHA VVG+Y+  KK K
Sbjct: 241 KANCIDSTLEAPVVIASELNKLKKDKLKPLEQVSLEPFERDHAVVVGVYRPVKKSK 296

[Close]

BLAST of MS000425 vs. ExPASy Swiss-Prot
Match: Q22053 (rRNA 2'-O-methyltransferase fibrillarin OS=Caenorhabditis elegans OX=6239 GN=fib-1 PE=2 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 9.4e-15
Identity = 36/57 (63.16%), Postives = 46/57 (80.70%), Query Frame = 0


Query: 2   QANCIDSTVPAETVFASEVNKLKADQFKPIERVTLEPFERDHACVVGIYQAPKKQKL 59
           +ANCIDST   E VFA EVNKLK ++FKP+E+VTLEP+ERDHA VV +Y+  K +K+
Sbjct: 296 KANCIDSTAEPEAVFAGEVNKLKEEKFKPLEQVTLEPYERDHAVVVAVYRPVKGKKV 352

[Close]

BLAST of MS000425 vs. ExPASy Swiss-Prot
Match: Q80WS3 (rRNA/tRNA 2'-O-methyltransferase fibrillarin-like protein 1 OS=Mus musculus OX=10090 GN=Fbll1 PE=1 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 2.1e-14
Identity = 36/56 (64.29%), Postives = 44/56 (78.57%), Query Frame = 0


Query: 2   QANCIDSTVPAETVFASEVNKLKADQFKPIERVTLEPFERDHACVVGIYQAPKKQK 58
           +ANCIDST  AE VFASEV KL+ +  KP E++TLEP+ERDHA VVG+Y+ P K K
Sbjct: 259 KANCIDSTASAEAVFASEVRKLQQENLKPQEQLTLEPYERDHAVVVGVYRPPPKSK 314

[Close]

BLAST of MS000425 vs. ExPASy TrEMBL
Match: A0A6J1JA27 (probable mediator of RNA polymerase II transcription subunit 36b OS=Cucurbita maxima OX=3661 GN=LOC111483086 PE=3 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.1e-21
Identity = 53/59 (89.83%), Postives = 56/59 (94.92%), Query Frame = 0


Query: 2   QANCIDSTVPAETVFASEVNKLKADQFKPIERVTLEPFERDHACVVGIYQAPKKQKLAA 61
           +ANCIDSTVPAE VFASEVNKLKADQFKP E+VTLEPFERDHACVVGIY+APKKQK AA
Sbjct: 246 KANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKAAA 304

[Close]

BLAST of MS000425 vs. ExPASy TrEMBL
Match: A0A5D3CYG2 (Mediator of RNA polymerase II transcription subunit 36a-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold477G00260 PE=3 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.1e-21
Identity = 53/59 (89.83%), Postives = 56/59 (94.92%), Query Frame = 0


Query: 2  QANCIDSTVPAETVFASEVNKLKADQFKPIERVTLEPFERDHACVVGIYQAPKKQKLAA 61
          +ANCIDSTVPAE VFASEVNKLKADQFKP E+VTLEPFERDHACVVGIY+APKKQK AA
Sbjct: 25 KANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKAAA 83

[Close]

BLAST of MS000425 vs. ExPASy TrEMBL
Match: A0A0A0L9V7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G183360 PE=3 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.1e-21
Identity = 53/59 (89.83%), Postives = 56/59 (94.92%), Query Frame = 0


Query: 2   QANCIDSTVPAETVFASEVNKLKADQFKPIERVTLEPFERDHACVVGIYQAPKKQKLAA 61
           +ANCIDSTVPAE VFASEVNKLKADQFKP E+VTLEPFERDHACVVGIY+APKKQK AA
Sbjct: 249 KANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKAAA 307

[Close]

BLAST of MS000425 vs. ExPASy TrEMBL
Match: A0A0A0KLF9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G174640 PE=3 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.1e-21
Identity = 53/59 (89.83%), Postives = 56/59 (94.92%), Query Frame = 0


Query: 2   QANCIDSTVPAETVFASEVNKLKADQFKPIERVTLEPFERDHACVVGIYQAPKKQKLAA 61
           +ANCIDSTVPAE VFASEVNKLKADQFKP E+VTLEPFERDHACVVGIY+APKKQK AA
Sbjct: 249 KANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKAAA 307

[Close]

BLAST of MS000425 vs. ExPASy TrEMBL
Match: A0A6J1E4P7 (probable mediator of RNA polymerase II transcription subunit 36b OS=Cucurbita moschata OX=3662 GN=LOC111430753 PE=3 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.1e-21
Identity = 53/59 (89.83%), Postives = 56/59 (94.92%), Query Frame = 0


Query: 2   QANCIDSTVPAETVFASEVNKLKADQFKPIERVTLEPFERDHACVVGIYQAPKKQKLAA 61
           +ANCIDSTVPAE VFASEVNKLKADQFKP E+VTLEPFERDHACVVGIY+APKKQK AA
Sbjct: 246 KANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKAAA 304

[Close]

BLAST of MS000425 vs. TAIR 10
Match: AT4G25630.1 (fibrillarin 2 )

HSP 1 Score: 92.4 bits (228), Expect = 1.3e-19
Identity = 43/58 (74.14%), Postives = 49/58 (84.48%), Query Frame = 0


Query: 2   QANCIDSTVPAETVFASEVNKLKADQFKPIERVTLEPFERDHACVVGIYQAPKKQKLA 60
           +ANCIDSTVPAE VF +EV KL+ +QFKP E+VTLEPFERDHACVVG Y+ PKK K A
Sbjct: 260 KANCIDSTVPAEAVFQTEVKKLQQEQFKPAEQVTLEPFERDHACVVGGYRMPKKPKAA 317

[Close]

BLAST of MS000425 vs. TAIR 10
Match: AT5G52470.1 (fibrillarin 1 )

HSP 1 Score: 92.4 bits (228), Expect = 1.3e-19
Identity = 44/59 (74.58%), Postives = 49/59 (83.05%), Query Frame = 0


Query: 2   QANCIDSTVPAETVFASEVNKLKADQFKPIERVTLEPFERDHACVVGIYQAPKKQKLAA 61
           +ANCIDSTV AE VF SEV KL+ +QFKP E+VTLEPFERDHACVVG Y+ PKKQK  A
Sbjct: 249 KANCIDSTVAAEAVFQSEVKKLQQEQFKPAEQVTLEPFERDHACVVGGYRMPKKQKTPA 307

[Close]

BLAST of MS000425 vs. TAIR 10
Match: AT5G52490.1 (Fibrillarin family protein )

HSP 1 Score: 57.4 bits (137), Expect = 4.6e-09
Identity = 26/54 (48.15%), Postives = 37/54 (68.52%), Query Frame = 0


Query: 2   QANCIDSTVPAETVFASEVNKLKADQFKPIERVTLEPFERDHACVVGIYQAPKK 56
           +AN IDST+ AETV+  EV KL+ ++ +P E + L+  E  HACV G Y+ P+K
Sbjct: 239 KANSIDSTIAAETVYQMEVEKLQMEELRPTEILHLDSCEEKHACVFGGYRLPRK 292

The following BLAST results are available for this feature:

BLAST of MS000425 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN57431.2	2.2e-21	89.83	hypothetical protein Csa_009607 [Cucumis sativus]	[more]
XP_022922917.1	2.2e-21	89.83	probable mediator of RNA polymerase II transcription subunit 36b [Cucurbita mosc...	[more]
XP_023552409.1	2.2e-21	89.83	mediator of RNA polymerase II transcription subunit 36a-like [Cucurbita pepo sub...	[more]
XP_004143621.2	2.2e-21	89.83	probable mediator of RNA polymerase II transcription subunit 36b [Cucumis sativu...	[more]
XP_038875959.1	2.2e-21	89.83	rRNA 2'-O-methyltransferase fibrillarin 1-like [Benincasa hispida]	[more]

BLAST of MS000425 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FEF8	1.8e-18	74.58	rRNA 2'-O-methyltransferase fibrillarin 1 OS=Arabidopsis thaliana OX=3702 GN=FIB...	[more]
Q94AH9	1.8e-18	74.14	rRNA 2'-O-methyltransferase fibrillarin 2 OS=Arabidopsis thaliana OX=3702 GN=FIB...	[more]
P35549	5.5e-15	66.07	rRNA 2'-O-methyltransferase fibrillarin OS=Leishmania major OX=5664 PE=3 SV=1	[more]
Q22053	9.4e-15	63.16	rRNA 2'-O-methyltransferase fibrillarin OS=Caenorhabditis elegans OX=6239 GN=fib...	[more]
Q80WS3	2.1e-14	64.29	rRNA/tRNA 2'-O-methyltransferase fibrillarin-like protein 1 OS=Mus musculus OX=1...	[more]

BLAST of MS000425 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JA27	1.1e-21	89.83	probable mediator of RNA polymerase II transcription subunit 36b OS=Cucurbita ma...	[more]
A0A5D3CYG2	1.1e-21	89.83	Mediator of RNA polymerase II transcription subunit 36a-like OS=Cucumis melo var...	[more]
A0A0A0L9V7	1.1e-21	89.83	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G183360 PE=3 SV=1	[more]
A0A0A0KLF9	1.1e-21	89.83	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G174640 PE=3 SV=1	[more]
A0A6J1E4P7	1.1e-21	89.83	probable mediator of RNA polymerase II transcription subunit 36b OS=Cucurbita mo...	[more]

BLAST of MS000425 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25630.1	1.3e-19	74.14	fibrillarin 2	[more]
AT5G52470.1	1.3e-19	74.58	fibrillarin 1	[more]
AT5G52490.1	4.6e-09	48.15	Fibrillarin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000692	Fibrillarin	PFAM	PF01269	Fibrillarin	coord: 2..51 e-value: 2.8E-15 score: 56.0
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 1..52 e-value: 5.7E-13 score: 50.8
None	No IPR available	PANTHER	PTHR10335:SF21	BNAA03G47570D PROTEIN	coord: 2..59
None	No IPR available	PANTHER	PTHR10335	RRNA 2-O-METHYLTRANSFERASE FIBRILLARIN	coord: 2..59

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS000425.1	MS000425.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006364	rRNA processing
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0003723	RNA binding