MS000291 (gene) Bitter gourd (TR) v1

Overview
NameMS000291
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionProtein RFT1 homolog
Locationscaffold44: 643854 .. 650906 (+)
RNA-Seq ExpressionMS000291
SyntenyMS000291
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGCATTATGATCTAGCTACTCAATTCCTGTCAAGAGGCATTCCATTCATCTTCAATTTGTGGATCGTGAGGCATCTTACAGAAGAAGATTATGCGGTACTTGTTCTTGTTCATCTCTCTTCTATTTGTTGGAATTTAGATGCATTGTCACTGAGCTCTTGTTGTCTTCCTCTTTTTTTCCCTCCATTTAAAATTTATTTATTTCAGAAATTTGGATTAGAAGCTCTGATCGGGTGCATTTTCTGTTATGTGAGCTTCATTTGTTTGTTTGGTTTGAAGATTTGACAATAGACCCAATGGTGAATTTTGAGCGGGTTATCTGTTATATTGTGTTTACAATTTGTTGATATTTCTTAAGCAGTGCTACATAACGTTGAAAAATTGGATCTTAATATTACTCACGACCATGTAAGTTAGCACCTCAGGAAGTCAATTGTGGAGACACAGCTAAAACGAAAAAGAAAAAGTGGATTTATCAAATATTGTTTAACGCTTGAAATTTTCTTACGGGAAAACTCTCTCCCCAAAAAGGTGGTTGGAATTTAGAACTATTATCTTCAAATTTGCGGTCTTGATAAATGTTTTCTGTAAATGCAGGTTTATGCAGTACAATTTCATCTTTTTGTTACATGTGTTCTGTTTCTCAGCCGGGAGGGATTCCGGCGAGCATGCATGCGGGCAGAGTTAAGGTACATCCTCACAATAATAGGTGCTGCCTTATGCACCCCCCCAACCGCCATATTTAGCACTTTGCAGTTAGCGTTTGTTATATTTGGCTCAATAAAATATTCTCAATGAAATTACCACATATTTGTGTATATTCATTCCTATTACTGGAGCTTTGTACTGAAATTGCTTTTGCTCTCCATTACACAGTTCTTTGCCATTATATACTTCTTAAAAAATGTTGATTTCATGCCTGCTTTCCATTTGAATGGCTTATACTGTGGTTTTTTTCTCCCCCAACAATTACATAGGTCTATTGACTAGAACACCAGTAATTATGATTTTGGATTGTTTTTATACTTGCTTTTATTTCTCTTTTTAAACATATTATGCCCCTTTAAGTATGAGAATGGGAAAGTTGCTGAATTTTATATGTGTGTCTACTACATTTATTAAATTTCTTTTGTAATGTTGGTTTATAATTCTTTGTTGCTGTATTCCAACTACAAATTTAATGCTCTTGAGATTAATTTTATATTATGCAGTGACAGCACTTCAGTGGAGGAAAATAGTAGGAGGCTACTTAAAGTAGCCTGGTTGAGTTTCCCACTTGGATTGGTTATTACTCTTGCTGCATTTTATCTTGTTCTTTGGTGGCAAGATATTGTCTTTTCTAATCCATATGCACAAGCCATCTTAATTAATGGTAATCCTATTCCTTTTTGCTTCCAACCTTTATATACCGTGTTTCGGCACCTTCTAATTTTCTTTTATTTTCTCGTGGGTATCAGTATTTTTAAATACATCAAACAATTCATGGCGTCAATAGAAGCAAAATACTTAAATTGGGATAGAATTTTGGGCAGAGTGGAGTATCTCCGAGTGATAATAGAAAATTAGAACTATTACCGAGTCACCAACTTGGTCTAGTGTCAAATTTTGACATATGTTACTACATCGTTGTTAAATCGTTTGGAATTGCTACAAGTCTAGTTTGACTTTATAAGTTAACCTGTGTTATTCATTTTAGAATCATATTTTGCAAAATGTATATGTGAACATCCATAATAGAAGTGAAAGGTTGCAGCAACTGATGATATATTATTATGGATAATTACATATTGGAGACCTTTTTTCTTTAAGTAAACTCTCGAACATTAATTACATATTGGAGACCTTTTTTCTTTAAGTAAACTCTCGAACATTAATTACATATTGGAGACCTTTTTTCTTTAAGTAAACTCTCGAACATTAACTAAATCTTACAACGTTATTTTTCCACCTTTGTAATTTTGTCCATCTTTTATGGCAGGATGTGCGTGTATTTTAGAGCTGTTGGCAGAGCCCCTATACATTCTTTCTCAGACTTTGCTGCTTCTTAAATTGCGGCTTCTAGTTGAAACTGTGGCTACATTTTCACGGTGCATGACAATGTATATCCTCCTGGTGAAGCGAGGTAACATGGTAAGATCTGCTAGACTGTCAATAATAATATTCAACCTGGAAAGCCTAAGACATCCTCCCTCTTGGCTATCGTGTTTTCCATATCTCCCATCCCCCAAGAGTCGGTCAGTTTCTTATTGTATATGGCGCTGCACTTTTGTATGGAATATTGTGCATTATTAAGACTGTGATGCATTCGCTGATAAATGTCCTAGAGCTGCAACATGGTGCATGCTTCTTAGTCAATTACAGCCGAAGTCAATGAGTCTCGTAGTTTATTCATGTGTTATACTCTCATCTCAGACCTCATAACAATTATATATTAGGTGCTGCAACTAATTAGAGTTCTTTGGCTTCTGGCAGGAGAAAGGAATCATATTTGCTTTGTCACAATCTGCATATGGTGCTTGCTTGTTCCTAGGATACTGGGTCTACTTTCTTTCTTTCCGTGAATTTAAATGGTCAGATCTTTTTCCTTTCAGGTACTTATCATTATGTTGACATATTATGCACTACGTTTCCTTACGGGTTGTTAACTATTTTGGTTGGCATTGAACAAAAATTCTAATTCTCTCCCTGAAATTTTTTTATGTATCAGCCATAAGCATGTTTTGCAAGAATCTACACAAACATGCATTTTTTGCACCTCAGTTTTGTTTAATATATATTTGATTCAGAATGCTTTCGCTGGAGAAAACCATTTTGAAATTACATGCAATTTGTTTTGCAGAGAAGGACATGTAGTTTGCATCGTTTTTACTAAACTTGAAATGAGATGATCAGTGCAATGAGGCTGTATCTCTTGAACAAAGTTAGTTCAAGTTAAATCTTTGTCGGCATTAATTTTTTTAATTGCCACTTTGCAATTCTATTTTCATTTAAGGATAGCATACACCCCGCTTATTCTTTACTTTGTTGGAAATATGTGGTTTATATGGAAATATTTTTGCAGAATGGGAAACGTGAAGGATTTTGATAAGAAGCTTTCAAACATGTGCCTATTATTTACTTTTCAATCCTTCAGAAAGTTGGTTCTTCAAGAAGGTGAAAAGATGGTTCTTGTGTGGTTAGATACACCATATAACCAAGCTGTTTATGGATTGGTAGATAAATTGGGTAAGTCCTGGGAGTGGTTTCGAAGTTTTTTAACTTCAGCTGACAAGATCGTGAAGATGTTGATATTCTACTACCTTCAACTTGATAAGCATGCTAATAGTAGTTATTTACTTAATTTTCAGGGAGTTTAGTGGTGCGACTGGTTTTTCTTCCATTTGAAGAAAGCTCATACACTACATTTGCAAGGTCTGCATCAGGTTTATATATTAAACTTTCTTCCTGTTTTTTATTTTAATTAGAAACTGAGCTTTCATTAAGACAAATGAAAGAATGTACACGAGCATAGAAAAAACCAACCTAACCAAAGGAAGCAAAAAACAAATTACAAAAGAAATGGGTTTTTGTTATATGTCTTTGTTCTCTAACCAGATCCCCTTGGCTTGTTCAAGTCTTTGATCCAAGAATAGTGCATTGATTCAAACCAGAGATAAAGTTCGAAATAAGAAACGTCTTTATGATTTTTTGTCTAGTATCACTGTTCAACCTATTTGGATTACCATTGAGGTCTAGTTTATTTGCTCATCATTCTTCCTTCTACTTTTGATATCCTTTATCCTCCTCCCACTCTCTTGTTTCTTTTCCCCTATTATCCCTTGCTCTCTCCTTTACTTATGTCGTCAATGAGGAAGGTAAAATATTGATTTTATTCAAGATACCAAGTGGGAAAAAGGATTCTCTTAGTATTCACTATTCAGCACCTCCATTAGAACACAACAACGGGAGACAAGGTTGGTTTTCTATCCTGGTGTGCCTAGAAAAAAGGTTGGGCAGAACTATGCAATCTAGTAGAGATGATGATCTTGCCTAACTAGCAGTCTAGTAGAGAAGATGGTTTTGCCTTCTTATTCTTTATCAACACTATTCGCTTATTCAGAACAATTTATGGTTATTTTACATTTCTGAAAATTGTTTGTGCTTTCCTGAACTATTATCACGCTAACCCCAGAAAGAAAAGAAAAAAATCCGTCTCACGACTTCAAAATTTTCATTTACTGTTTTGACTCAATTTGATAGGAGAATACCCAGATAAGAGCAGAAAACTTGGTATCTGCCTTTCAGAAGCGCTCAAGTTAGTTTTGCTGATTGGTATGCAACTTCAATTAATTATACACTCATAGTCTATATATAATTATTTCTGATTTGTCCTCATTTTCATGATCTGTTTTTTTTCTTTGCACAATGCACACAATAAAAATTTGATGATATTTTTGCCATTGAATAATTAATTTGAGCACAATTTCATTAAAGACATTAAGGCAGATAGTGTCTGCTTCACCAAAGATCTTCTAATATTCTTCATCTTGTTGACTAACTTAGGTACTTCAACGACAATAATCTTTGCCCTATTACTCTCTAAATTTGGCTGGTATTATTAGCATATGAGACTCAGTTGAATGGTTATGGAATTCATTTTATTATGTTAAAATTGTATTGAGAATATGGTCAATGCTTAGAATATTAAATATCCTATGAGTTTTTCAATATCAAATTTAGTAGGTTCTATTGGTTATTTTGTGGGATTAATCGATGTACATATGAAATCGTTGATATGGTCTGGATACCTACGATAATAAAAGAATTTAGAATTCTCTTTCCAGTTTGTTTATCTTTTGAAATGAAATATCTTCAATGTATATGCCTAATTTTTAAAATGGTGTTCAGCCTGCATGGAACATTTTCTAATCTCCCACCTATTACCCTTTGACTTCTTGGAAGTTATCTAGCAAAGCATTAAACCTCTGAGCTGGACCATTCTCTCACTTTGAGGTTGATCTTTAAAATGAATGTTATGATTTCATGTAGGCCTAGTATTTATGGCATTTGGTCCAAGCTATTCGTATGCTCTCATTAGGTTGTTGTATGGCCAGAAATGGAGTGATGGAGAAGCACCAACAGCTCTCCGGTACTATTGCCTGTATATCATTGTTCTGGCTATGAATGGTTAGTAATTTCATGTTTATTATTTTTAACTCTTATCTTTCTCTCTGCATATATATGAACAAGAAACTGCTAAATAGCTTTTGAATTTCTCATAGAAAAGTCAACTTTAGACTATGGCTCAAAATGAAGTTTCTGTCCATTTTTAGTTGCTTCATTTGAATTGGATTTGCCTGTATTTTAGATTATAAATAATAATTACTTCATAAATAGCTTTATCTGGAGAATACTAGAAGATGATACTCGAAGGAAATCTTAATTACTAAAACAAGTATATTATAGAGGATCTACACGCACAATGCACATTGAAACCTTTGGTATTGCAATGATTGATTTACCAAATTTTAAAAAAATGGTCAAGTTCACACTAGAAAAGTCTTGAAATTTGACAATGGATGCTGTTGAACGTAGACTGAACTCAGGACCAGATAATCCTTGTCCATGAGTAGCAGGCTGTGAGGCCTTAATTCGAATTATTGCTTATAATATGCTGGTCTAAAATAAATTCTAGAGTGAGCAAAATATCTTATTGTGAAAATATATATTGAAAGGAACGAATTTATGGAAGGAGGTGATAGGATTAGGCTACCACTTCACAAACTCTACAGCTAAATAGAAATCCCTCCAAGTTCTAACCAGCCATGATTAATAACGGAAAAAGATAAAAGAAGAAGGAAGTTACAAAAAAGTTTTGGTGGCAGAAATTCAACTAGAAGCATCTATAAATATACATGCACCTAATATTTCCTAATCCTGACTACTAGACTACTCGAATTTTACATCAGGGACTTCAGAAGCATTTCTACATGCTGTTGCAAATGAGAGCCAACTCAAGAAGTCAAATGACTCATTGCTTCTATTCTCATTGATCTATGTTATGCTAAATTTTCTGCTGATCCGGTCATCGGGAGTAGTTGGCTTGATTTTTGCAAATTCTATAAGTATCCTTTTCCAATGATTGTGAGATGGTTCATTTAGCCTTCTGGTTTTTACTTTCAACTTTGTTATTTTGTTGGTAACATTTGTTTTTGTTTTGTGTTTTGAGCTCTTGGCTGTGAGGACACTTCTTCACATGTTAGTATGATCATTTTAGATTGTACTTCCATAATCCAATTCAAGTGTACTGTTCAACTCAGTTTATTTCGTGCAAACCATACTATGCTAGCTACACACCTTTGAAACCATGTTCTCTCTCAATATACTCAAGGGAACTGTTTCTGTACTCTTTGATCATAATGTCTTTGCTAAACATCTAATTTATATTTCATGTCAGTATGGATAAATTCAGGTATATGATTATTAGAAGTCTACGCCTGGTCTCATCTTAAGATATTCTAATAAACTAACACCCTCAACATTGAGGAAAAGATGGTTTATTGTGGAATATAGATATGTTTAATAAATGGTTAGTTCCTCATGCAATTCACTTTTTAAGTTACAACTTCCTGATATTTCTTTAGAAGATGGTACCATTTTACATTCCATCATGACATAGGACATAGAGAACAAAGTTTATGAATACTCAAATGATAAAGATACTCATTGAAGAAATGAAACTTTTGTAGTATATAATTTTCTTGACTGTTATAAAAGATATGATTTTGAGGATAACGTACTCTGCCATATTCATCAAACGCTACTTCAAGGTATGCTTTGATTAGAAGTGTAACATTTTTTGTTTTCAATTCTTTATCATTAACAAATGTTAGTTTGACCCTACAGAATCCTCCATTTTCCTTCAGCGGCTGCTTGCCTTCAGGATGGATATTTTTATTATTCTCAGGGGTTCTGACCCTTATTTCAGAGAGACTGTTTCAAGACGAGCAGAACTTTTGGTCAACTTTTCCACTTCATTTTTCGATTGGTTTGGTTTGCTTCCTTGTGTCATTA

mRNA sequence

TTGCATTATGATCTAGCTACTCAATTCCTGTCAAGAGGCATTCCATTCATCTTCAATTTGTGGATCGTGAGGCATCTTACAGAAGAAGATTATGCGGTTTATGCAGTACAATTTCATCTTTTTGTTACATGTGTTCTGTTTCTCAGCCGGGAGGGATTCCGGCGAGCATGCATGCGGGCAGAGTTAAGTGACAGCACTTCAGTGGAGGAAAATAGTAGGAGGCTACTTAAAGTAGCCTGGTTGAGTTTCCCACTTGGATTGGTTATTACTCTTGCTGCATTTTATCTTGTTCTTTGGTGGCAAGATATTGTCTTTTCTAATCCATATGCACAAGCCATCTTAATTAATGGATGTGCGTGTATTTTAGAGCTGTTGGCAGAGCCCCTATACATTCTTTCTCAGACTTTGCTGCTTCTTAAATTGCGGCTTCTAGTTGAAACTGTGGCTACATTTTCACGGTGCATGACAATGTATATCCTCCTGGTGAAGCGAGGTAACATGGAGAAAGGAATCATATTTGCTTTGTCACAATCTGCATATGGTGCTTGCTTGTTCCTAGGATACTGGGTCTACTTTCTTTCTTTCCGTGAATTTAAATGGTCAGATCTTTTTCCTTTCAGAATGGGAAACGTGAAGGATTTTGATAAGAAGCTTTCAAACATGTGCCTATTATTTACTTTTCAATCCTTCAGAAAGTTGGTTCTTCAAGAAGGTGAAAAGATGGTTCTTGTGTGGTTAGATACACCATATAACCAAGCTGTTTATGGATTGGTAGATAAATTGGGTAATTTAGTGGTGCGACTGGTTTTTCTTCCATTTGAAGAAAGCTCATACACTACATTTGCAAGGTCTGCATCAGGAGAATACCCAGATAAGAGCAGAAAACTTGGTATCTGCCTTTCAGAAGCGCTCAAGTTAGTTTTGCTGATTGGCCTAGTATTTATGGCATTTGGTCCAAGCTATTCGTATGCTCTCATTAGGTTGTTGTATGGCCAGAAATGGAGTGATGGAGAAGCACCAACAGCTCTCCGGTACTATTGCCTGTATATCATTGTTCTGGCTATGAATGGGACTTCAGAAGCATTTCTACATGCTGTTGCAAATGAGAGCCAACTCAAGAAGTCAAATGACTCATTGCTTCTATTCTCATTGATCTATGTTATGCTAAATTTTCTGCTGATCCGGTCATCGGGAGTAGTTGGCTTGATTTTTGCAAATTCTATAAATATGATTTTGAGGATAACGTACTCTGCCATATTCATCAAACGCTACTTCAAGAATCCTCCATTTTCCTTCAGCGGCTGCTTGCCTTCAGGATGGATATTTTTATTATTCTCAGGGGTTCTGACCCTTATTTCAGAGAGACTGTTTCAAGACGAGCAGAACTTTTGGTCAACTTTTCCACTTCATTTTTCGATTGGTTTGGTTTGCTTCCTTGTGTCATTA

Coding sequence (CDS)

TTGCATTATGATCTAGCTACTCAATTCCTGTCAAGAGGCATTCCATTCATCTTCAATTTGTGGATCGTGAGGCATCTTACAGAAGAAGATTATGCGGTTTATGCAGTACAATTTCATCTTTTTGTTACATGTGTTCTGTTTCTCAGCCGGGAGGGATTCCGGCGAGCATGCATGCGGGCAGAGTTAAGTGACAGCACTTCAGTGGAGGAAAATAGTAGGAGGCTACTTAAAGTAGCCTGGTTGAGTTTCCCACTTGGATTGGTTATTACTCTTGCTGCATTTTATCTTGTTCTTTGGTGGCAAGATATTGTCTTTTCTAATCCATATGCACAAGCCATCTTAATTAATGGATGTGCGTGTATTTTAGAGCTGTTGGCAGAGCCCCTATACATTCTTTCTCAGACTTTGCTGCTTCTTAAATTGCGGCTTCTAGTTGAAACTGTGGCTACATTTTCACGGTGCATGACAATGTATATCCTCCTGGTGAAGCGAGGTAACATGGAGAAAGGAATCATATTTGCTTTGTCACAATCTGCATATGGTGCTTGCTTGTTCCTAGGATACTGGGTCTACTTTCTTTCTTTCCGTGAATTTAAATGGTCAGATCTTTTTCCTTTCAGAATGGGAAACGTGAAGGATTTTGATAAGAAGCTTTCAAACATGTGCCTATTATTTACTTTTCAATCCTTCAGAAAGTTGGTTCTTCAAGAAGGTGAAAAGATGGTTCTTGTGTGGTTAGATACACCATATAACCAAGCTGTTTATGGATTGGTAGATAAATTGGGTAATTTAGTGGTGCGACTGGTTTTTCTTCCATTTGAAGAAAGCTCATACACTACATTTGCAAGGTCTGCATCAGGAGAATACCCAGATAAGAGCAGAAAACTTGGTATCTGCCTTTCAGAAGCGCTCAAGTTAGTTTTGCTGATTGGCCTAGTATTTATGGCATTTGGTCCAAGCTATTCGTATGCTCTCATTAGGTTGTTGTATGGCCAGAAATGGAGTGATGGAGAAGCACCAACAGCTCTCCGGTACTATTGCCTGTATATCATTGTTCTGGCTATGAATGGGACTTCAGAAGCATTTCTACATGCTGTTGCAAATGAGAGCCAACTCAAGAAGTCAAATGACTCATTGCTTCTATTCTCATTGATCTATGTTATGCTAAATTTTCTGCTGATCCGGTCATCGGGAGTAGTTGGCTTGATTTTTGCAAATTCTATAAATATGATTTTGAGGATAACGTACTCTGCCATATTCATCAAACGCTACTTCAAGAATCCTCCATTTTCCTTCAGCGGCTGCTTGCCTTCAGGATGGATATTTTTATTATTCTCAGGGGTTCTGACCCTTATTTCAGAGAGACTGTTTCAAGACGAGCAGAACTTTTGGTCAACTTTTCCACTTCATTTTTCGATTGGTTTGGTTTGCTTCCTTGTGTCATTA

Protein sequence

LHYDLATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAELSDSTSVEENSRRLLKVAWLSFPLGLVITLAAFYLVLWWQDIVFSNPYAQAILINGCACILELLAEPLYILSQTLLLLKLRLLVETVATFSRCMTMYILLVKRGNMEKGIIFALSQSAYGACLFLGYWVYFLSFREFKWSDLFPFRMGNVKDFDKKLSNMCLLFTFQSFRKLVLQEGEKMVLVWLDTPYNQAVYGLVDKLGNLVVRLVFLPFEESSYTTFARSASGEYPDKSRKLGICLSEALKLVLLIGLVFMAFGPSYSYALIRLLYGQKWSDGEAPTALRYYCLYIIVLAMNGTSEAFLHAVANESQLKKSNDSLLLFSLIYVMLNFLLIRSSGVVGLIFANSINMILRITYSAIFIKRYFKNPPFSFSGCLPSGWIFLLFSGVLTLISERLFQDEQNFWSTFPLHFSIGLVCFLVSL
Homology
BLAST of MS000291 vs. NCBI nr
Match: XP_022141197.1 (protein RFT1 homolog isoform X2 [Momordica charantia])

HSP 1 Score: 932.9 bits (2410), Expect = 1.1e-267
Identity = 475/480 (98.96%), Postives = 479/480 (99.79%), Query Frame = 0

Query: 3   YDLATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL 62
           Y +ATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL
Sbjct: 26  YLMATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL 85

Query: 63  SDSTSVEENSRRLLKVAWLSFPLGLVITLAAFYLVLWWQDIVFSNPYAQAILINGCACIL 122
           SDSTSVEENSRRLLKVAWLSFP+GLVITLAAFYLV+WWQDIVFSNPYAQAILINGCACIL
Sbjct: 86  SDSTSVEENSRRLLKVAWLSFPVGLVITLAAFYLVIWWQDIVFSNPYAQAILINGCACIL 145

Query: 123 ELLAEPLYILSQTLLLLKLRLLVETVATFSRCMTMYILLVKRGNMEKGIIFALSQSAYGA 182
           ELLAEPLYILSQTLLLLKLRLLVETVATFSRCMTMYILLVKRGNMEKGIIFALSQSAYGA
Sbjct: 146 ELLAEPLYILSQTLLLLKLRLLVETVATFSRCMTMYILLVKRGNMEKGIIFALSQSAYGA 205

Query: 183 CLFLGYWVYFLSFREFKWSDLFPFRMGNVKDFDKKLSNMCLLFTFQSFRKLVLQEGEKMV 242
           CLFLGYWVYFLSFREFKWSDLFPFRMGNVKDFDKKLSNMCLLFTFQSFRKLVLQEGEKMV
Sbjct: 206 CLFLGYWVYFLSFREFKWSDLFPFRMGNVKDFDKKLSNMCLLFTFQSFRKLVLQEGEKMV 265

Query: 243 LVWLDTPYNQAVYGLVDKLGNLVVRLVFLPFEESSYTTFARSASGEYPDKSRKLGICLSE 302
           LVWLDTPYNQAVYGLVDKLG+LVVRLVFLPFEESSYTTFARSASGEYPDKSRKLGICLSE
Sbjct: 266 LVWLDTPYNQAVYGLVDKLGSLVVRLVFLPFEESSYTTFARSASGEYPDKSRKLGICLSE 325

Query: 303 ALKLVLLIGLVFMAFGPSYSYALIRLLYGQKWSDGEAPTALRYYCLYIIVLAMNGTSEAF 362
           ALKLVLLIGLVFMAFGPSYSYALIRLLYGQKWSDGEAPTALRYYCLYIIVLAMNGTSEAF
Sbjct: 326 ALKLVLLIGLVFMAFGPSYSYALIRLLYGQKWSDGEAPTALRYYCLYIIVLAMNGTSEAF 385

Query: 363 LHAVANESQLKKSNDSLLLFSLIYVMLNFLLIRSSGVVGLIFANSINMILRITYSAIFIK 422
           LHAVANESQLKKSNDSLLLFSLIYVMLNFLLIRSSGVVGLIFANSINMILRITYSAIFIK
Sbjct: 386 LHAVANESQLKKSNDSLLLFSLIYVMLNFLLIRSSGVVGLIFANSINMILRITYSAIFIK 445

Query: 423 RYFKNPPFSFSGCLPSGWIFLLFSGVLTLISERLFQDEQNFWSTFPLHFSIGLVCFLVSL 482
           RYFKNPPFSFSGCLPSGWIFLLFSGVLTLISERLFQDEQNFWSTFPLHFSIGLVCFLVSL
Sbjct: 446 RYFKNPPFSFSGCLPSGWIFLLFSGVLTLISERLFQDEQNFWSTFPLHFSIGLVCFLVSL 505

BLAST of MS000291 vs. NCBI nr
Match: XP_023552765.1 (protein RFT1 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 889.8 bits (2298), Expect = 1.0e-254
Identity = 452/480 (94.17%), Postives = 467/480 (97.29%), Query Frame = 0

Query: 3   YDLATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL 62
           Y +ATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL
Sbjct: 27  YLMATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL 86

Query: 63  SDSTSVEENSRRLLKVAWLSFPLGLVITLAAFYLVLWWQDIVFSNPYAQAILINGCACIL 122
           SDSTSVEENSRRLLKVAWLSFPLGLVITLAA YLVLWWQDI FSNPYAQAILINGCACIL
Sbjct: 87  SDSTSVEENSRRLLKVAWLSFPLGLVITLAACYLVLWWQDIPFSNPYAQAILINGCACIL 146

Query: 123 ELLAEPLYILSQTLLLLKLRLLVETVATFSRCMTMYILLVKRGNMEKGIIFALSQSAYGA 182
           ELLAEPLYILSQTLLLLKLRLLVET+ATFSRCMTMYILLVK  NMEKGIIF+LSQSAYGA
Sbjct: 147 ELLAEPLYILSQTLLLLKLRLLVETMATFSRCMTMYILLVKSSNMEKGIIFSLSQSAYGA 206

Query: 183 CLFLGYWVYFLSFREFKWSDLFPFRMGNVKDFDKKLSNMCLLFTFQSFRKLVLQEGEKMV 242
           CLFLGYWVYF+ FR FKWSDLFPFR+GN+KDFDKKLSNMC+LFT QSFRKLVLQEGEKMV
Sbjct: 207 CLFLGYWVYFIYFRAFKWSDLFPFRIGNMKDFDKKLSNMCILFTLQSFRKLVLQEGEKMV 266

Query: 243 LVWLDTPYNQAVYGLVDKLGNLVVRLVFLPFEESSYTTFARSASGEYPDKSRKLGICLSE 302
           LVWLDTPYNQAVYGLVDKLG+LVVRLVFLPFEESSYTTFARSASGE+PDK+RKLGICLSE
Sbjct: 267 LVWLDTPYNQAVYGLVDKLGSLVVRLVFLPFEESSYTTFARSASGEHPDKTRKLGICLSE 326

Query: 303 ALKLVLLIGLVFMAFGPSYSYALIRLLYGQKWSDGEAPTALRYYCLYIIVLAMNGTSEAF 362
           ALKLVLLIGL+FMAFGPSYSYALIRLLYGQKWSDGEAPTALRYYCLYIIVLAMNGTSEAF
Sbjct: 327 ALKLVLLIGLIFMAFGPSYSYALIRLLYGQKWSDGEAPTALRYYCLYIIVLAMNGTSEAF 386

Query: 363 LHAVANESQLKKSNDSLLLFSLIYVMLNFLLIRSSGVVGLIFANSINMILRITYSAIFIK 422
           LHAVANESQLKKSNDSLLLFSLIYVMLNFLLIRSSGVVGLIFANSINMILRITYSA+FIK
Sbjct: 387 LHAVANESQLKKSNDSLLLFSLIYVMLNFLLIRSSGVVGLIFANSINMILRITYSAVFIK 446

Query: 423 RYFKNPPFSFSGCLPSGWIFLLFSGVLTLISERLFQDEQNFWSTFPLHFSIGLVCFLVSL 482
           RYFKN  FSF+ CLPSGWIFLLFSGVLTLISERLF D+QNFWSTF LHFSIGL+CFLVSL
Sbjct: 447 RYFKNSSFSFNSCLPSGWIFLLFSGVLTLISERLFLDQQNFWSTFLLHFSIGLICFLVSL 506

BLAST of MS000291 vs. NCBI nr
Match: XP_022922967.1 (protein RFT1 homolog [Cucurbita moschata] >KAG6576812.1 Protein RFT1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 885.2 bits (2286), Expect = 2.6e-253
Identity = 450/480 (93.75%), Postives = 465/480 (96.88%), Query Frame = 0

Query: 3   YDLATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL 62
           Y +ATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL
Sbjct: 27  YLMATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL 86

Query: 63  SDSTSVEENSRRLLKVAWLSFPLGLVITLAAFYLVLWWQDIVFSNPYAQAILINGCACIL 122
           SDSTSVEENSRRLLKVAWLSFPLGLVITLAA YLVLWWQDI FSNPYAQAILINGCACIL
Sbjct: 87  SDSTSVEENSRRLLKVAWLSFPLGLVITLAACYLVLWWQDIPFSNPYAQAILINGCACIL 146

Query: 123 ELLAEPLYILSQTLLLLKLRLLVETVATFSRCMTMYILLVKRGNMEKGIIFALSQSAYGA 182
           ELLAEPLYILSQTLLLLKLRLLVET+ATFSRCMTMYILLVK  NMEKGIIF+LSQSAYGA
Sbjct: 147 ELLAEPLYILSQTLLLLKLRLLVETMATFSRCMTMYILLVKSSNMEKGIIFSLSQSAYGA 206

Query: 183 CLFLGYWVYFLSFREFKWSDLFPFRMGNVKDFDKKLSNMCLLFTFQSFRKLVLQEGEKMV 242
           CLFLGYWVYF+ FR F+WSDLFPFR+GN+KDFDKKLSNMC+LFT QSFRKLVLQEGEKMV
Sbjct: 207 CLFLGYWVYFIYFRAFRWSDLFPFRIGNMKDFDKKLSNMCILFTLQSFRKLVLQEGEKMV 266

Query: 243 LVWLDTPYNQAVYGLVDKLGNLVVRLVFLPFEESSYTTFARSASGEYPDKSRKLGICLSE 302
           LVWLDTPYNQAVYGLVDKLG+LVVRLVFLPFEESSYTTFARSASGE+PDK+RKLGICLSE
Sbjct: 267 LVWLDTPYNQAVYGLVDKLGSLVVRLVFLPFEESSYTTFARSASGEHPDKTRKLGICLSE 326

Query: 303 ALKLVLLIGLVFMAFGPSYSYALIRLLYGQKWSDGEAPTALRYYCLYIIVLAMNGTSEAF 362
           ALKLVLLIGL+FMAFGPSYSYALIRLLYGQKWSDGEAPTALRYYCLYIIVLAMNGTSEAF
Sbjct: 327 ALKLVLLIGLIFMAFGPSYSYALIRLLYGQKWSDGEAPTALRYYCLYIIVLAMNGTSEAF 386

Query: 363 LHAVANESQLKKSNDSLLLFSLIYVMLNFLLIRSSGVVGLIFANSINMILRITYSAIFIK 422
           LHAVANESQLKKSNDSLLLFSLIYVMLNFLLIRSSGVVGLIFANSINMILRITYSA+FIK
Sbjct: 387 LHAVANESQLKKSNDSLLLFSLIYVMLNFLLIRSSGVVGLIFANSINMILRITYSAVFIK 446

Query: 423 RYFKNPPFSFSGCLPSGWIFLLFSGVLTLISERLFQDEQNFWSTFPLHFSIGLVCFLVSL 482
           RYFKN  FSF+ CLPSGWIFLLFSGVLTLISERLF D+QNFWSTF LHF IGL CFLVSL
Sbjct: 447 RYFKNSSFSFNSCLPSGWIFLLFSGVLTLISERLFLDQQNFWSTFLLHFLIGLTCFLVSL 506

BLAST of MS000291 vs. NCBI nr
Match: XP_038894185.1 (protein RFT1 homolog [Benincasa hispida])

HSP 1 Score: 884.8 bits (2285), Expect = 3.4e-253
Identity = 451/479 (94.15%), Postives = 464/479 (96.87%), Query Frame = 0

Query: 3   YDLATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL 62
           Y +ATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL
Sbjct: 27  YLMATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL 86

Query: 63  SDSTSVEENSRRLLKVAWLSFPLGLVITLAAFYLVLWWQDIVFSNPYAQAILINGCACIL 122
           SDSTSVEENSRRLLKVAWLSFPLGLVITLAA YLVLWWQDI FSNPYAQAILINGCACIL
Sbjct: 87  SDSTSVEENSRRLLKVAWLSFPLGLVITLAACYLVLWWQDIAFSNPYAQAILINGCACIL 146

Query: 123 ELLAEPLYILSQTLLLLKLRLLVETVATFSRCMTMYILLVKRGNMEKGIIFALSQSAYGA 182
           ELLAEPLYILSQTLL LKLRLLVET+ATFSRCMTMYILLVKR NMEKGIIFALSQSAYGA
Sbjct: 147 ELLAEPLYILSQTLLFLKLRLLVETMATFSRCMTMYILLVKRSNMEKGIIFALSQSAYGA 206

Query: 183 CLFLGYWVYFLSFREFKWSDLFPFRMGNVKDFDKKLSNMCLLFTFQSFRKLVLQEGEKMV 242
           CLFLGYWVYFL+F  FKWSDLFPFR+GN+KDFDKKLSNMC+LFT QSFRKLVLQEGEKMV
Sbjct: 207 CLFLGYWVYFLNFCAFKWSDLFPFRVGNMKDFDKKLSNMCILFTLQSFRKLVLQEGEKMV 266

Query: 243 LVWLDTPYNQAVYGLVDKLGNLVVRLVFLPFEESSYTTFARSASGEYPDKSRKLGICLSE 302
           LVWLDTPYNQAVYGLVDKLG+LVVRLVFLPFEESSYTTFARSASGEYPDK+RKL ICLSE
Sbjct: 267 LVWLDTPYNQAVYGLVDKLGSLVVRLVFLPFEESSYTTFARSASGEYPDKTRKLAICLSE 326

Query: 303 ALKLVLLIGLVFMAFGPSYSYALIRLLYGQKWSDGEAPTALRYYCLYIIVLAMNGTSEAF 362
           ALKLVLLIGL+FMAFGPSYSYALIRLLYGQKWSDG+APTALRYYCLYIIVLAMNGTSEAF
Sbjct: 327 ALKLVLLIGLIFMAFGPSYSYALIRLLYGQKWSDGDAPTALRYYCLYIIVLAMNGTSEAF 386

Query: 363 LHAVANESQLKKSNDSLLLFSLIYVMLNFLLIRSSGVVGLIFANSINMILRITYSAIFIK 422
           LHAVANESQLKKSNDSLLLFSLIYV+LNFLLIRSSGVVGLIFANSINMILRITYSAIFIK
Sbjct: 387 LHAVANESQLKKSNDSLLLFSLIYVVLNFLLIRSSGVVGLIFANSINMILRITYSAIFIK 446

Query: 423 RYFKNPPFSFSGCLPSGWIFLLFSGVLTLISERLFQDEQNFWSTFPLHFSIGLVCFLVS 482
            YFKN  FSF+ CLPSGWIFLLFSGVLTLISERLF D+QNFWSTF LHFSIGLVCFLVS
Sbjct: 447 GYFKNSSFSFNRCLPSGWIFLLFSGVLTLISERLFLDQQNFWSTFSLHFSIGLVCFLVS 505

BLAST of MS000291 vs. NCBI nr
Match: XP_022985478.1 (protein RFT1 homolog [Cucurbita maxima])

HSP 1 Score: 879.4 bits (2271), Expect = 1.4e-251
Identity = 446/480 (92.92%), Postives = 465/480 (96.88%), Query Frame = 0

Query: 3   YDLATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL 62
           Y +ATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL
Sbjct: 16  YLMATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL 75

Query: 63  SDSTSVEENSRRLLKVAWLSFPLGLVITLAAFYLVLWWQDIVFSNPYAQAILINGCACIL 122
           SDSTSVEENSRRLLKVAWLSFPLGLVITLAA YLVLWWQDI FSNPYAQAILINGCACIL
Sbjct: 76  SDSTSVEENSRRLLKVAWLSFPLGLVITLAACYLVLWWQDIPFSNPYAQAILINGCACIL 135

Query: 123 ELLAEPLYILSQTLLLLKLRLLVETVATFSRCMTMYILLVKRGNMEKGIIFALSQSAYGA 182
           ELLAEPLYILSQTLLLLKLRLLVET+ATFSRC+TMYILLVK  NMEKGIIF+LSQSAYGA
Sbjct: 136 ELLAEPLYILSQTLLLLKLRLLVETMATFSRCITMYILLVKSSNMEKGIIFSLSQSAYGA 195

Query: 183 CLFLGYWVYFLSFREFKWSDLFPFRMGNVKDFDKKLSNMCLLFTFQSFRKLVLQEGEKMV 242
           CLFLGYWVYF+ FR F+WSDLFPFR+GN+KDFDKKLSNMC+LFT QSFRKLVLQEGEKMV
Sbjct: 196 CLFLGYWVYFIYFRAFRWSDLFPFRIGNMKDFDKKLSNMCILFTLQSFRKLVLQEGEKMV 255

Query: 243 LVWLDTPYNQAVYGLVDKLGNLVVRLVFLPFEESSYTTFARSASGEYPDKSRKLGICLSE 302
           LVWLDTPYNQAVYGLVDKLG+LVVRLVFLPFEESSYTTFARSASGE+PDK+RKLGICLSE
Sbjct: 256 LVWLDTPYNQAVYGLVDKLGSLVVRLVFLPFEESSYTTFARSASGEHPDKTRKLGICLSE 315

Query: 303 ALKLVLLIGLVFMAFGPSYSYALIRLLYGQKWSDGEAPTALRYYCLYIIVLAMNGTSEAF 362
           ALKLVLLIGL+FMAFGPSYSYALIRLLYGQKWSDGEAPTALRYYCLYIIVLAMNGTSEAF
Sbjct: 316 ALKLVLLIGLIFMAFGPSYSYALIRLLYGQKWSDGEAPTALRYYCLYIIVLAMNGTSEAF 375

Query: 363 LHAVANESQLKKSNDSLLLFSLIYVMLNFLLIRSSGVVGLIFANSINMILRITYSAIFIK 422
           LHAVANESQLKKSNDSLLLFSLIYV+LNFLLIRSSGVVGLIFANSINMILRITYSA+FIK
Sbjct: 376 LHAVANESQLKKSNDSLLLFSLIYVVLNFLLIRSSGVVGLIFANSINMILRITYSAVFIK 435

Query: 423 RYFKNPPFSFSGCLPSGWIFLLFSGVLTLISERLFQDEQNFWSTFPLHFSIGLVCFLVSL 482
           RYFKN  FSF+ CLPSGWIFLLFSGVLTLISER+F D++NFWSTF LHF IGL CFLVSL
Sbjct: 436 RYFKNSSFSFNSCLPSGWIFLLFSGVLTLISERVFLDQKNFWSTFLLHFLIGLTCFLVSL 495

BLAST of MS000291 vs. ExPASy Swiss-Prot
Match: Q54IV7 (Protein RFT1 homolog OS=Dictyostelium discoideum OX=44689 GN=rft1 PE=3 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 7.0e-60
Identity = 164/522 (31.42%), Postives = 262/522 (50.19%), Query Frame = 0

Query: 3   YDLATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL 62
           Y +  Q +SR   FI N  ++  + +  + V A+Q+ L  + +LFLSRE  RRAC R  +
Sbjct: 20  YLIGLQIISRLFTFIINTLVIVGVDDSIFGVSAIQYQLLSSIILFLSREAIRRACTRVNI 79

Query: 63  SDSTSVEENSRRLLKVAWLSFP--LGLVITLAAFYLVLWWQDIVFSNPYAQAILINGCAC 122
           +D  + + N + ++ ++WL  P  +GL I    F+L    ++ +    Y   + +   + 
Sbjct: 80  TDKLNNDNNLKSVINLSWLVLPIGIGLSIIFENFFLYTSTKETLEILNYHYGLRLFTISS 139

Query: 123 ILELLAEPLYILSQTLLLLKLRLLVETVATFSRCMTMYILLVKRGNMEKGII-FALSQSA 182
           ILELL+EP+YIL+Q LLL K+R  VE  A F +  + Y  +V    +  G+I F  +Q  
Sbjct: 140 ILELLSEPMYILAQNLLLFKIRTTVEGFALFFKTFSTYYFIV---ILNMGLIGFGYAQIL 199

Query: 183 YGACLFLGYWVYFL----------SFREF-----KWSDLFPFRMGNVKDFDKKLSNMCLL 242
           Y   L +GY+ YFL            +EF         LFP     +   D+ L  + LL
Sbjct: 200 YSLTLVIGYFGYFLINIINNNKNKDNKEFSNCFKSIDQLFPKFSTRI---DRNLIKLSLL 259

Query: 243 FTFQSFRKLVLQEGEKMVLVWLDTPYNQAVYGLVDKLGNLVVRLVFLPFEESSYTTFAR- 302
           +T+QS  KL+LQEGEK VL + +T   QA++ +V  LG+L+VR +FLP EE+ +  F + 
Sbjct: 260 YTWQSIYKLLLQEGEKFVLFFSETNQGQAIFAIVSNLGSLIVRFLFLPIEETCFLMFPKL 319

Query: 303 ------------------------SASGEYPDKSRKLGICLSEALKLVLLIGLVFMAFGP 362
                                     +    D  +     L   +K ++L+ LVF  FGP
Sbjct: 320 FPTINNNNNNNNNNNNNNNNNNKNQENNNNNDDFKNGANVLIVIMKFLILVSLVFTCFGP 379

Query: 363 SYSYALIRLLYGQKWSDGEAPTALRYYCLYIIVLAMNGTSEAFLHAVANESQLKKSNDSL 422
            +S+ L+ LLY  K+ D  A   L +YC+Y+  LA+NG SE+F+H+VA E QLK  N  L
Sbjct: 380 GFSHLLLNLLYNNKFRDTNAGVLLGFYCIYVGFLAINGVSESFVHSVAKEDQLKTVNWVL 439

Query: 423 LLFSLIYVMLNFLLIRSSGVVGLIFANSINMILRITYSAIFIKRYFKNPPFSFSGCLPSG 480
           ++   IY++   +  +    +G+I AN +N+ L                    S  +P+ 
Sbjct: 440 IIIGFIYLLFTLIFCKLFQNIGIILANCLNIKL--------------------SNMIPNK 499

BLAST of MS000291 vs. ExPASy Swiss-Prot
Match: Q0D2E8 (Protein RFT1 homolog OS=Xenopus tropicalis OX=8364 GN=rft1 PE=2 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 1.2e-59
Identity = 172/507 (33.93%), Postives = 268/507 (52.86%), Query Frame = 0

Query: 3   YDLATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL 62
           Y +  Q L R + F  N + +R++++E   +  V+  LF T V+FL+RE FRRAC+    
Sbjct: 16  YSVILQILFRVLTFALNAFTLRYVSKEIIGIVNVRLTLFYTTVVFLAREAFRRACL---- 75

Query: 63  SDSTSVEENSRRLLKVAWLSFPLGLVITLAAFYLVLWWQDIVFSNP-----YAQAILING 122
             S S +++ R  + + WL+ PLG+  +    +  +W Q +    P     Y   +   G
Sbjct: 76  --SHSAQQSWRHTIHLTWLAVPLGICWSFILGW--IWLQILEVPEPEAIPYYNIGVWAFG 135

Query: 123 CACILELLAEPLYILSQTLLLLKLRLLVETVATFSRCMTMYILLVKRGNMEKGIIFALSQ 182
            + ++ELLAEP ++L+Q  L +KL+++ E++A   RC ++ ++LV        +IF+L+Q
Sbjct: 136 FSAVVELLAEPFWVLAQAHLFVKLKVVAESLAIIIRC-SVTVILVLLCPQWGLLIFSLAQ 195

Query: 183 SAYGACLFLGYWVYFLSFREFKWSDLFPFRMGNVKDF-----------DKKLSNMCLLFT 242
             Y + L L Y  YF  F     ++  PF +  +++F           D K + +   F 
Sbjct: 196 VLYTSALALCYIAYFARFLGSLEAEKKPFPLRRMREFLPRFSSSQAFLDWKQAWLAWSFF 255

Query: 243 FQSFRKLVLQEGEKMVLVWLDTPY--NQAVYGLVDKLGNLVVRLVFLPFEESSYTTFARS 302
            QSF K +L EGE+ V+ +L+     +Q VY +V+ LG+LV R +FLP EES Y  FA+ 
Sbjct: 256 KQSFLKQILTEGERYVMTFLNVLSFGDQGVYDIVNNLGSLVARFIFLPIEESFYVFFAKV 315

Query: 303 AS-GEYPDKSRKLGI-----CLSEALKLVLLIGLVFMAFGPSYSYALIRLLYGQKWSDGE 362
              G+     RK  I      L   LKLV LIGLV +AFG +YS+  + +  G   S G 
Sbjct: 316 LERGKKVQSQRKEEISMASEVLESLLKLVTLIGLVIIAFGYAYSHLALDIYGGSMLSGGS 375

Query: 363 APTALRYYCLYIIVLAMNGTSEAFLHAVANESQLKKSNDSLLLFSLIYVMLNFLLIRSSG 422
            P  LR YCLY+++LA+NG +E F  A   +  + + N  +L  SL ++ L++ +    G
Sbjct: 376 GPVLLRCYCLYVLLLAINGVTECFTFASMGKEDVDRYNYVMLGLSLSFLCLSYYMTLWLG 435

Query: 423 VVGLIFANSINMILRITYSAIFIKRYFKNPPFS-FSGCLPSGWI--FLLFSGVLTLISER 482
            VG I AN  NM LRIT+S ++I RY+K  P+    G LPS  +   L  S  +T  SE 
Sbjct: 436 SVGFILANCFNMGLRITHSLLYIMRYYKGSPYQPLIGLLPSPVVVCVLAVSAAVTGYSEV 495

BLAST of MS000291 vs. ExPASy Swiss-Prot
Match: Q96AA3 (Protein RFT1 homolog OS=Homo sapiens OX=9606 GN=RFT1 PE=1 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 6.3e-53
Identity = 162/506 (32.02%), Postives = 254/506 (50.20%), Query Frame = 0

Query: 5   LATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAELSD 64
           L  Q L R I F+ N +I+R L++E   V  V+  L  +  LFL+RE FRRAC+      
Sbjct: 18  LLLQVLFRLITFVLNAFILRFLSKEIVGVVNVRLTLLYSTTLFLAREAFRRACL------ 77

Query: 65  STSVEENSRRLLKVAWLSFPLGLVITLAAFYLVLWWQDIVFSNP-----YAQAILINGCA 124
           S   + +  + L + WL+ PLG+  +L  F   +W Q +   +P     YA  +++ G +
Sbjct: 78  SGGTQRDWSQTLNLLWLTVPLGVFWSL--FLGWIWLQLLEVPDPNVVPHYATGVVLFGLS 137

Query: 125 CILELLAEPLYILSQTLLLLKLRLLVETVATFSRCMTMYILLVKRGNMEKGIIFALSQSA 184
            ++ELL EP ++L+Q  + +KL+++ E+++   + +    L++   +     IF+L+Q  
Sbjct: 138 AVVELLGEPFWVLAQAHMFVKLKVIAESLSVILKSVLTAFLVLWLPHWGL-YIFSLAQLF 197

Query: 185 YGACLFLGYWVYFL------------SFREFKWSDLFPFRMGNVKDFDKKLSNMCLLFTF 244
           Y   L L Y +YF             +    + +DL P    N    + K + +   F  
Sbjct: 198 YTTVLVLCYVIYFTKLLGSPESTKLQTLPVSRITDLLPNITRNGAFINWKEAKLTWSFFK 257

Query: 245 QSFRKLVLQEGEKMVLVWLDTPY--NQAVYGLVDKLGNLVVRLVFLPFEESSYTTFA--- 304
           QSF K +L EGE+ V+ +L+     +Q VY +V+ LG+LV RL+F P EES Y  FA   
Sbjct: 258 QSFLKQILTEGERYVMTFLNVLNFGDQGVYDIVNNLGSLVARLIFQPIEESFYIFFAKVL 317

Query: 305 ---RSASGEYPDKSRKLGICLSEALKLVLLIGLVFMAFGPSYSYALIRLLYGQKWSDGEA 364
              + A+ +  +        L   LKL LL GL    FG +YS   + +  G   S G  
Sbjct: 318 ERGKDATLQKQEDVAVAAAVLESLLKLALLAGLTITVFGFAYSQLALDIYGGTMLSSGSG 377

Query: 365 PTALRYYCLYIIVLAMNGTSEAFLHAVANESQLKKSNDSLLLFSLIYVMLNFLLIRSSGV 424
           P  LR YCLY+++LA+NG +E F  A  ++ ++ + N  +L  S  +++L++LL R  G 
Sbjct: 378 PVLLRSYCLYVLLLAINGVTECFTFAAMSKEEVDRYNFVMLALSSSFLVLSYLLTRWCGS 437

Query: 425 VGLIFANSINMILRITYSAIFIKRYFKNPPFSFSGCLPSGWIFL---LFSGVLTLISERL 483
           VG I AN  NM +RIT S  FI RY++  P      L    + L     SG +T +SE  
Sbjct: 438 VGFILANCFNMGIRITQSLCFIHRYYRRSPHRPLAGLHLSPVLLGTFALSGGVTAVSEVF 497

BLAST of MS000291 vs. ExPASy Swiss-Prot
Match: Q8C3B8 (Protein RFT1 homolog OS=Mus musculus OX=10090 GN=Rft1 PE=2 SV=2)

HSP 1 Score: 205.7 bits (522), Expect = 1.2e-51
Identity = 159/507 (31.36%), Postives = 256/507 (50.49%), Query Frame = 0

Query: 5   LATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAELSD 64
           L  Q L R I F+ N +I+R L++E   +  V+  L  +   FL+RE FRRAC+      
Sbjct: 18  LLLQVLFRLITFVLNAFILRFLSKEIVGIVNVRLTLLYSTTTFLAREAFRRACL------ 77

Query: 65  STSVEENSRRLLKVAWLSFPLGLVITLAAFYLVLWWQDIVFSNP-----YAQAILINGCA 124
           S   + +  + L + WL+ PLG+  +    +  +W Q +   +P     Y   +L  G +
Sbjct: 78  SGGAQRDWSQTLNLLWLTVPLGIFWSSCLGW--VWLQLLEVPDPDVVPYYGTGVLFFGLS 137

Query: 125 CILELLAEPLYILSQTLLLLKLRLLVETVATFSRCMTMYILLVKRGNMEKGIIFALSQSA 184
            ++ELL EP ++L+Q  + +KL++L E+++   R +   +L++   +     IF+L+Q  
Sbjct: 138 AVVELLGEPFWVLAQAHMFVKLKVLAESMSVILRSVLTALLVLWLPHWGL-YIFSLAQLL 197

Query: 185 YGACLFLGYWVYF------------LSFREFKWSDLFPFRMGNVKDFDKKLSNMCLLFTF 244
           Y   L L Y +Y             L+    + + L P    +    + K + +   F  
Sbjct: 198 YTTVLVLCYAIYLIQLLRSPESAKQLTLPVSRVTQLLPSISRSRAFVNWKEAGLAWSFFK 257

Query: 245 QSFRKLVLQEGEKMVLVWLDTPY--NQAVYGLVDKLGNLVVRLVFLPFEESSYTTFA--- 304
           QSF K +L EGE+ V+ +L+     +Q VY +V+ LG+LV RL+F P EES Y  FA   
Sbjct: 258 QSFLKQILTEGERYVMTFLNVLNFGDQGVYDIVNNLGSLVARLIFQPVEESFYLFFAKVL 317

Query: 305 ---RSASGEYPDKSRKLGICLSEALKLVLLIGLVFMAFGPSYSYALIRLLYGQKWSDGEA 364
              + AS +  D        L   LKL LL GL    FG +YS   + +  G   S G  
Sbjct: 318 EREKDASLQKQDDVAVAAAVLESLLKLALLTGLTMTVFGFAYSQLALDIYGGAMLSSGSG 377

Query: 365 PTALRYYCLYIIVLAMNGTSEAFLHAVANESQLKKSNDSLLLFSLIYVMLNFLLIRSSGV 424
           P  +R YCLY+++LA+NG +E F+ A  ++ ++ + N ++L  S  +++L++LL    G 
Sbjct: 378 PVLMRCYCLYVLLLAINGVTECFMFAAMSKEEVDRYNFTMLALSSSFLVLSYLLTSWCGS 437

Query: 425 VGLIFANSINMILRITYSAIFIKRYFKNPPF-SFSGCLPSG---WIFLLFSGVLTLISER 483
           VG I AN  NM +RIT S  FI  YF+  P    +G   S     +F+L +G+ T +SE 
Sbjct: 438 VGFIMANCFNMGIRITQSLSFIHHYFRESPHRPLAGLRLSPVLLGVFILSAGI-TSVSEA 497

BLAST of MS000291 vs. ExPASy Swiss-Prot
Match: P38206 (Oligosaccharide translocation protein RFT1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RFT1 PE=1 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 2.5e-33
Identity = 131/478 (27.41%), Postives = 228/478 (47.70%), Query Frame = 0

Query: 8   QFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAELSDSTS 67
           Q  ++ + FI N  ++R L+   + + A         VLF SR+  R + +R   S +  
Sbjct: 30  QLFTKLVTFILNNLLIRFLSPRIFGITAF-LEFIQGTVLFFSRDAIRLSTLRISDSGNGI 89

Query: 68  VEENSR----------RLLKVA--------WLSFPL--GLVI----TLAAFYLVLWWQDI 127
           ++++            ++L+ A        W+ FPL  GL+      + A+++ L +   
Sbjct: 90  IDDDDEEEYQETHYKSKVLQTAVNFAYIPFWIGFPLSIGLIAWQYRNINAYFITLPF--- 149

Query: 128 VFSNPYAQAILINGCACILELLAEPLYILSQTLLLLKLRLLVETVATFSRCMTMYILL-- 187
                +  +I +   + I+ELL+EP +I++Q +L    R   E++A  + C+  +I++  
Sbjct: 150 -----FRWSIFLIWLSIIVELLSEPFFIVNQFMLNYAARSRFESIAVTTGCIVNFIVVYA 209

Query: 188 VKRGNMEKGII-------------FALSQSAYGACLFLGYWVYFLSFREFKWSDLFPFRM 247
           V++     G++             FAL + A+   L   Y+  +L  + FK   LF  R+
Sbjct: 210 VQQSRYPMGVVTSDIDKEGIAILAFALGKLAHSITLLACYYWDYL--KNFKPKKLFSTRL 269

Query: 248 GNV---------KDFDKKLSNMCLLFTFQSFRKL--------VLQEGEKMVLVWLDTPYN 307
             +         K + K  S        Q F+K+        +L EG+K+++  L T   
Sbjct: 270 TKIKTRENNELKKGYPKSTSYFFQNDILQHFKKVYFQLCFKHLLTEGDKLIINSLCTVEE 329

Query: 308 QAVYGLVDKLGNLVVRLVFLPFEESSYTTFARSASGEYPDKSRKLGI-CLSEALKLVLLI 367
           Q +Y L+   G+L+ RL+F P EES     AR  S   P K+ KL I  L    +  + +
Sbjct: 330 QGIYALLSNYGSLLTRLLFAPIEESLRLFLARLLSSHNP-KNLKLSIEVLVNLTRFYIYL 389

Query: 368 GLVFMAFGPSYSYALIRLLYGQKWSDGEAPTALRYYCLYIIVLAMNGTSEAFLHAVANES 427
            L+ + FGP+ S  L++ L G KWS       +R YC YI  L++NG  EAF  +VA   
Sbjct: 390 SLMIIVFGPANSSFLLQFLIGSKWSTTSVLDTIRVYCFYIPFLSLNGIFEAFFQSVATGD 449

BLAST of MS000291 vs. ExPASy TrEMBL
Match: A0A6J1CJ71 (Protein RFT1 homolog OS=Momordica charantia OX=3673 GN=LOC111011652 PE=3 SV=1)

HSP 1 Score: 932.9 bits (2410), Expect = 5.2e-268
Identity = 475/480 (98.96%), Postives = 479/480 (99.79%), Query Frame = 0

Query: 3   YDLATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL 62
           Y +ATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL
Sbjct: 26  YLMATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL 85

Query: 63  SDSTSVEENSRRLLKVAWLSFPLGLVITLAAFYLVLWWQDIVFSNPYAQAILINGCACIL 122
           SDSTSVEENSRRLLKVAWLSFP+GLVITLAAFYLV+WWQDIVFSNPYAQAILINGCACIL
Sbjct: 86  SDSTSVEENSRRLLKVAWLSFPVGLVITLAAFYLVIWWQDIVFSNPYAQAILINGCACIL 145

Query: 123 ELLAEPLYILSQTLLLLKLRLLVETVATFSRCMTMYILLVKRGNMEKGIIFALSQSAYGA 182
           ELLAEPLYILSQTLLLLKLRLLVETVATFSRCMTMYILLVKRGNMEKGIIFALSQSAYGA
Sbjct: 146 ELLAEPLYILSQTLLLLKLRLLVETVATFSRCMTMYILLVKRGNMEKGIIFALSQSAYGA 205

Query: 183 CLFLGYWVYFLSFREFKWSDLFPFRMGNVKDFDKKLSNMCLLFTFQSFRKLVLQEGEKMV 242
           CLFLGYWVYFLSFREFKWSDLFPFRMGNVKDFDKKLSNMCLLFTFQSFRKLVLQEGEKMV
Sbjct: 206 CLFLGYWVYFLSFREFKWSDLFPFRMGNVKDFDKKLSNMCLLFTFQSFRKLVLQEGEKMV 265

Query: 243 LVWLDTPYNQAVYGLVDKLGNLVVRLVFLPFEESSYTTFARSASGEYPDKSRKLGICLSE 302
           LVWLDTPYNQAVYGLVDKLG+LVVRLVFLPFEESSYTTFARSASGEYPDKSRKLGICLSE
Sbjct: 266 LVWLDTPYNQAVYGLVDKLGSLVVRLVFLPFEESSYTTFARSASGEYPDKSRKLGICLSE 325

Query: 303 ALKLVLLIGLVFMAFGPSYSYALIRLLYGQKWSDGEAPTALRYYCLYIIVLAMNGTSEAF 362
           ALKLVLLIGLVFMAFGPSYSYALIRLLYGQKWSDGEAPTALRYYCLYIIVLAMNGTSEAF
Sbjct: 326 ALKLVLLIGLVFMAFGPSYSYALIRLLYGQKWSDGEAPTALRYYCLYIIVLAMNGTSEAF 385

Query: 363 LHAVANESQLKKSNDSLLLFSLIYVMLNFLLIRSSGVVGLIFANSINMILRITYSAIFIK 422
           LHAVANESQLKKSNDSLLLFSLIYVMLNFLLIRSSGVVGLIFANSINMILRITYSAIFIK
Sbjct: 386 LHAVANESQLKKSNDSLLLFSLIYVMLNFLLIRSSGVVGLIFANSINMILRITYSAIFIK 445

Query: 423 RYFKNPPFSFSGCLPSGWIFLLFSGVLTLISERLFQDEQNFWSTFPLHFSIGLVCFLVSL 482
           RYFKNPPFSFSGCLPSGWIFLLFSGVLTLISERLFQDEQNFWSTFPLHFSIGLVCFLVSL
Sbjct: 446 RYFKNPPFSFSGCLPSGWIFLLFSGVLTLISERLFQDEQNFWSTFPLHFSIGLVCFLVSL 505

BLAST of MS000291 vs. ExPASy TrEMBL
Match: A0A6J1E4U9 (Protein RFT1 homolog OS=Cucurbita moschata OX=3662 GN=LOC111430792 PE=3 SV=1)

HSP 1 Score: 885.2 bits (2286), Expect = 1.2e-253
Identity = 450/480 (93.75%), Postives = 465/480 (96.88%), Query Frame = 0

Query: 3   YDLATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL 62
           Y +ATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL
Sbjct: 27  YLMATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL 86

Query: 63  SDSTSVEENSRRLLKVAWLSFPLGLVITLAAFYLVLWWQDIVFSNPYAQAILINGCACIL 122
           SDSTSVEENSRRLLKVAWLSFPLGLVITLAA YLVLWWQDI FSNPYAQAILINGCACIL
Sbjct: 87  SDSTSVEENSRRLLKVAWLSFPLGLVITLAACYLVLWWQDIPFSNPYAQAILINGCACIL 146

Query: 123 ELLAEPLYILSQTLLLLKLRLLVETVATFSRCMTMYILLVKRGNMEKGIIFALSQSAYGA 182
           ELLAEPLYILSQTLLLLKLRLLVET+ATFSRCMTMYILLVK  NMEKGIIF+LSQSAYGA
Sbjct: 147 ELLAEPLYILSQTLLLLKLRLLVETMATFSRCMTMYILLVKSSNMEKGIIFSLSQSAYGA 206

Query: 183 CLFLGYWVYFLSFREFKWSDLFPFRMGNVKDFDKKLSNMCLLFTFQSFRKLVLQEGEKMV 242
           CLFLGYWVYF+ FR F+WSDLFPFR+GN+KDFDKKLSNMC+LFT QSFRKLVLQEGEKMV
Sbjct: 207 CLFLGYWVYFIYFRAFRWSDLFPFRIGNMKDFDKKLSNMCILFTLQSFRKLVLQEGEKMV 266

Query: 243 LVWLDTPYNQAVYGLVDKLGNLVVRLVFLPFEESSYTTFARSASGEYPDKSRKLGICLSE 302
           LVWLDTPYNQAVYGLVDKLG+LVVRLVFLPFEESSYTTFARSASGE+PDK+RKLGICLSE
Sbjct: 267 LVWLDTPYNQAVYGLVDKLGSLVVRLVFLPFEESSYTTFARSASGEHPDKTRKLGICLSE 326

Query: 303 ALKLVLLIGLVFMAFGPSYSYALIRLLYGQKWSDGEAPTALRYYCLYIIVLAMNGTSEAF 362
           ALKLVLLIGL+FMAFGPSYSYALIRLLYGQKWSDGEAPTALRYYCLYIIVLAMNGTSEAF
Sbjct: 327 ALKLVLLIGLIFMAFGPSYSYALIRLLYGQKWSDGEAPTALRYYCLYIIVLAMNGTSEAF 386

Query: 363 LHAVANESQLKKSNDSLLLFSLIYVMLNFLLIRSSGVVGLIFANSINMILRITYSAIFIK 422
           LHAVANESQLKKSNDSLLLFSLIYVMLNFLLIRSSGVVGLIFANSINMILRITYSA+FIK
Sbjct: 387 LHAVANESQLKKSNDSLLLFSLIYVMLNFLLIRSSGVVGLIFANSINMILRITYSAVFIK 446

Query: 423 RYFKNPPFSFSGCLPSGWIFLLFSGVLTLISERLFQDEQNFWSTFPLHFSIGLVCFLVSL 482
           RYFKN  FSF+ CLPSGWIFLLFSGVLTLISERLF D+QNFWSTF LHF IGL CFLVSL
Sbjct: 447 RYFKNSSFSFNSCLPSGWIFLLFSGVLTLISERLFLDQQNFWSTFLLHFLIGLTCFLVSL 506

BLAST of MS000291 vs. ExPASy TrEMBL
Match: A0A6J1JDQ3 (Protein RFT1 homolog OS=Cucurbita maxima OX=3661 GN=LOC111483475 PE=3 SV=1)

HSP 1 Score: 879.4 bits (2271), Expect = 6.8e-252
Identity = 446/480 (92.92%), Postives = 465/480 (96.88%), Query Frame = 0

Query: 3   YDLATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL 62
           Y +ATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL
Sbjct: 16  YLMATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL 75

Query: 63  SDSTSVEENSRRLLKVAWLSFPLGLVITLAAFYLVLWWQDIVFSNPYAQAILINGCACIL 122
           SDSTSVEENSRRLLKVAWLSFPLGLVITLAA YLVLWWQDI FSNPYAQAILINGCACIL
Sbjct: 76  SDSTSVEENSRRLLKVAWLSFPLGLVITLAACYLVLWWQDIPFSNPYAQAILINGCACIL 135

Query: 123 ELLAEPLYILSQTLLLLKLRLLVETVATFSRCMTMYILLVKRGNMEKGIIFALSQSAYGA 182
           ELLAEPLYILSQTLLLLKLRLLVET+ATFSRC+TMYILLVK  NMEKGIIF+LSQSAYGA
Sbjct: 136 ELLAEPLYILSQTLLLLKLRLLVETMATFSRCITMYILLVKSSNMEKGIIFSLSQSAYGA 195

Query: 183 CLFLGYWVYFLSFREFKWSDLFPFRMGNVKDFDKKLSNMCLLFTFQSFRKLVLQEGEKMV 242
           CLFLGYWVYF+ FR F+WSDLFPFR+GN+KDFDKKLSNMC+LFT QSFRKLVLQEGEKMV
Sbjct: 196 CLFLGYWVYFIYFRAFRWSDLFPFRIGNMKDFDKKLSNMCILFTLQSFRKLVLQEGEKMV 255

Query: 243 LVWLDTPYNQAVYGLVDKLGNLVVRLVFLPFEESSYTTFARSASGEYPDKSRKLGICLSE 302
           LVWLDTPYNQAVYGLVDKLG+LVVRLVFLPFEESSYTTFARSASGE+PDK+RKLGICLSE
Sbjct: 256 LVWLDTPYNQAVYGLVDKLGSLVVRLVFLPFEESSYTTFARSASGEHPDKTRKLGICLSE 315

Query: 303 ALKLVLLIGLVFMAFGPSYSYALIRLLYGQKWSDGEAPTALRYYCLYIIVLAMNGTSEAF 362
           ALKLVLLIGL+FMAFGPSYSYALIRLLYGQKWSDGEAPTALRYYCLYIIVLAMNGTSEAF
Sbjct: 316 ALKLVLLIGLIFMAFGPSYSYALIRLLYGQKWSDGEAPTALRYYCLYIIVLAMNGTSEAF 375

Query: 363 LHAVANESQLKKSNDSLLLFSLIYVMLNFLLIRSSGVVGLIFANSINMILRITYSAIFIK 422
           LHAVANESQLKKSNDSLLLFSLIYV+LNFLLIRSSGVVGLIFANSINMILRITYSA+FIK
Sbjct: 376 LHAVANESQLKKSNDSLLLFSLIYVVLNFLLIRSSGVVGLIFANSINMILRITYSAVFIK 435

Query: 423 RYFKNPPFSFSGCLPSGWIFLLFSGVLTLISERLFQDEQNFWSTFPLHFSIGLVCFLVSL 482
           RYFKN  FSF+ CLPSGWIFLLFSGVLTLISER+F D++NFWSTF LHF IGL CFLVSL
Sbjct: 436 RYFKNSSFSFNSCLPSGWIFLLFSGVLTLISERVFLDQKNFWSTFLLHFLIGLTCFLVSL 495

BLAST of MS000291 vs. ExPASy TrEMBL
Match: A0A1S3BXG4 (Protein RFT1 homolog OS=Cucumis melo OX=3656 GN=LOC103494483 PE=3 SV=1)

HSP 1 Score: 879.0 bits (2270), Expect = 8.9e-252
Identity = 448/480 (93.33%), Postives = 462/480 (96.25%), Query Frame = 0

Query: 3   YDLATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL 62
           Y +ATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL
Sbjct: 27  YLMATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL 86

Query: 63  SDSTSVEENSRRLLKVAWLSFPLGLVITLAAFYLVLWWQDIVFSNPYAQAILINGCACIL 122
           SDSTSVEENSRRLLKVAWLSFPLGL ITLAA YLVLWWQ+I  SNPYAQAILINGCACIL
Sbjct: 87  SDSTSVEENSRRLLKVAWLSFPLGLFITLAACYLVLWWQNIPLSNPYAQAILINGCACIL 146

Query: 123 ELLAEPLYILSQTLLLLKLRLLVETVATFSRCMTMYILLVKRGNMEKGIIFALSQSAYGA 182
           ELLAEPLYILSQTLLLLKLRLLVET+ATFSRCMTMYILLV+  NMEKGIIFALSQSAYGA
Sbjct: 147 ELLAEPLYILSQTLLLLKLRLLVETMATFSRCMTMYILLVRWSNMEKGIIFALSQSAYGA 206

Query: 183 CLFLGYWVYFLSFREFKWSDLFPFRMGNVKDFDKKLSNMCLLFTFQSFRKLVLQEGEKMV 242
           CLFLGYWVYFL+FR FKWSDLFPFR+GN+KDFDKKLSNMC+LFT QSFRKLVLQEGEKMV
Sbjct: 207 CLFLGYWVYFLNFRAFKWSDLFPFRVGNMKDFDKKLSNMCILFTLQSFRKLVLQEGEKMV 266

Query: 243 LVWLDTPYNQAVYGLVDKLGNLVVRLVFLPFEESSYTTFARSASGEYPDKSRKLGICLSE 302
           LVWLDTPYNQAVYGLVDKLG+LVVRLVFLPFEESSYTTFARSASGEYPDK+RKL ICLSE
Sbjct: 267 LVWLDTPYNQAVYGLVDKLGSLVVRLVFLPFEESSYTTFARSASGEYPDKTRKLAICLSE 326

Query: 303 ALKLVLLIGLVFMAFGPSYSYALIRLLYGQKWSDGEAPTALRYYCLYIIVLAMNGTSEAF 362
           ALKLV+LIGL+FMAFGPSYSYALIRLLYGQKWSDGEAPTALRYYCLYIIVLAMNGTSEAF
Sbjct: 327 ALKLVVLIGLIFMAFGPSYSYALIRLLYGQKWSDGEAPTALRYYCLYIIVLAMNGTSEAF 386

Query: 363 LHAVANESQLKKSNDSLLLFSLIYVMLNFLLIRSSGVVGLIFANSINMILRITYSAIFIK 422
           LHAVANESQLKKSNDSLLLFSLIYVMLNFLLIRSSGVVGLIFANSINMILRITYSAIFIK
Sbjct: 387 LHAVANESQLKKSNDSLLLFSLIYVMLNFLLIRSSGVVGLIFANSINMILRITYSAIFIK 446

Query: 423 RYFKNPPFSFSGCLPSGWIFLLFSGVLTLISERLFQDEQNFWSTFPLHFSIGLVCFLVSL 482
            YFKN  FSF+ CLPSGWIFLL SGVLTLISERLF D+QNFWSTF LHFSIGL CFLVSL
Sbjct: 447 GYFKNSSFSFNSCLPSGWIFLLLSGVLTLISERLFMDQQNFWSTFSLHFSIGLACFLVSL 506

BLAST of MS000291 vs. ExPASy TrEMBL
Match: A0A0A0KW95 (Protein RFT1 homolog OS=Cucumis sativus OX=3659 GN=Csa_4G022850 PE=3 SV=1)

HSP 1 Score: 872.8 bits (2254), Expect = 6.4e-250
Identity = 445/480 (92.71%), Postives = 460/480 (95.83%), Query Frame = 0

Query: 3   YDLATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL 62
           Y +ATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL
Sbjct: 27  YLMATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL 86

Query: 63  SDSTSVEENSRRLLKVAWLSFPLGLVITLAAFYLVLWWQDIVFSNPYAQAILINGCACIL 122
           SDSTSVEENSRRLLKVAWLSFPLGL ITLAA  LVLWW++I  SNPYAQAILINGCACIL
Sbjct: 87  SDSTSVEENSRRLLKVAWLSFPLGLFITLAACNLVLWWRNIPLSNPYAQAILINGCACIL 146

Query: 123 ELLAEPLYILSQTLLLLKLRLLVETVATFSRCMTMYILLVKRGNMEKGIIFALSQSAYGA 182
           ELLAEPLYILSQTLLLLKLRLLVET+ATFSRCMTMYILLV+  NMEKGIIFALSQSAYGA
Sbjct: 147 ELLAEPLYILSQTLLLLKLRLLVETMATFSRCMTMYILLVRGSNMEKGIIFALSQSAYGA 206

Query: 183 CLFLGYWVYFLSFREFKWSDLFPFRMGNVKDFDKKLSNMCLLFTFQSFRKLVLQEGEKMV 242
           CLFLGYWVYFL+FR FKWSDLFPFR+GN+KDFDKKLSNMC+LFT QSFRKLVLQEGEKMV
Sbjct: 207 CLFLGYWVYFLNFRAFKWSDLFPFRVGNMKDFDKKLSNMCILFTLQSFRKLVLQEGEKMV 266

Query: 243 LVWLDTPYNQAVYGLVDKLGNLVVRLVFLPFEESSYTTFARSASGEYPDKSRKLGICLSE 302
           LVWLDTPYNQAVYGLVDKLG+LVVRLVFLPFEESSYTTFARSASGEYPDK+RKL ICLSE
Sbjct: 267 LVWLDTPYNQAVYGLVDKLGSLVVRLVFLPFEESSYTTFARSASGEYPDKTRKLAICLSE 326

Query: 303 ALKLVLLIGLVFMAFGPSYSYALIRLLYGQKWSDGEAPTALRYYCLYIIVLAMNGTSEAF 362
           ALKLV+LIGL+FMAFGPSYSYALIRLLYGQKWSDGEAPTALRYYCLYIIVLAMNGTSEAF
Sbjct: 327 ALKLVVLIGLIFMAFGPSYSYALIRLLYGQKWSDGEAPTALRYYCLYIIVLAMNGTSEAF 386

Query: 363 LHAVANESQLKKSNDSLLLFSLIYVMLNFLLIRSSGVVGLIFANSINMILRITYSAIFIK 422
           LHAVANESQLKKSNDSLLLFS IYVMLNFLLIRSSGVVGLIFANSINMILRITYSAIFIK
Sbjct: 387 LHAVANESQLKKSNDSLLLFSFIYVMLNFLLIRSSGVVGLIFANSINMILRITYSAIFIK 446

Query: 423 RYFKNPPFSFSGCLPSGWIFLLFSGVLTLISERLFQDEQNFWSTFPLHFSIGLVCFLVSL 482
            YFKNP FSF+ CLPSGWIFLL SGVLTLISERLF D+Q FWSTF LHFSIGL CFLVSL
Sbjct: 447 GYFKNPSFSFNSCLPSGWIFLLLSGVLTLISERLFLDQQKFWSTFSLHFSIGLACFLVSL 506

BLAST of MS000291 vs. TAIR 10
Match: AT5G07630.1 (lipid transporters )

HSP 1 Score: 668.3 bits (1723), Expect = 4.6e-192
Identity = 334/481 (69.44%), Postives = 399/481 (82.95%), Query Frame = 0

Query: 3   YDLATQFLSRGIPFIFNLWIVRHLTEEDYAVYAVQFHLFVTCVLFLSREGFRRACMRAEL 62
           + L+ QF+SR IPF+FN WIVRHLTE DYA+YA+QFHLFVTCVLFLSREGFRRAC+RA  
Sbjct: 106 FSLSRQFISRSIPFVFNSWIVRHLTERDYALYAIQFHLFVTCVLFLSREGFRRACLRAGT 165

Query: 63  S-DSTSVEENSRRLLKVAWLSFPLGLVITLAAFYLVLWWQDIVFSNPYAQAILINGCACI 122
             D    EEN  R+LKVAW++ PLG+ IT+AA   VLWWQ++ +S+PYAQAI I+G AC+
Sbjct: 166 KRDGPESEENVTRILKVAWVTVPLGIAITIAACIFVLWWQNLSYSDPYAQAIWIHGFACV 225

Query: 123 LELLAEPLYILSQTLLLLKLRLLVETVATFSRCMTMYILLVKRGNMEKGIIFALSQSAYG 182
           LEL+AEPLYILSQTLL L+LRL+VETVAT +RC+T+Y LLV + NMEK IIFALSQ AYG
Sbjct: 226 LELMAEPLYILSQTLLKLQLRLIVETVATLARCVTLYSLLVMQTNMEKVIIFALSQVAYG 285

Query: 183 ACLFLGYWVYFLSFREFKWSDLFPFRMGNVKDFDKKLSNMCLLFTFQSFRKLVLQEGEKM 242
             LF+GYW YFL    ++ S+LFPFR GN  DFD  LS MC LFTFQSFRKL+LQEGEK+
Sbjct: 286 GSLFIGYWAYFLICGVYRSSNLFPFRPGNFMDFDNDLSKMCKLFTFQSFRKLILQEGEKL 345

Query: 243 VLVWLDTPYNQAVYGLVDKLGNLVVRLVFLPFEESSYTTFARSASGEYPDKSRKLGICLS 302
           VLVWLDTPYNQAVYG+VDKLG+LVVR+VFLPFEESSYT FAR ASG+Y ++++KLGI L+
Sbjct: 346 VLVWLDTPYNQAVYGIVDKLGSLVVRMVFLPFEESSYTIFARFASGDYQERNKKLGIYLT 405

Query: 303 EALKLVLLIGLVFMAFGPSYSYALIRLLYGQKWSDGEAPTALRYYCLYIIVLAMNGTSEA 362
            ALKLV+LIGL+FMAFGPSYSY+LIRLLYG+KWSDGEA  AL++YCLYIIVLAMNGTSEA
Sbjct: 406 VALKLVILIGLIFMAFGPSYSYSLIRLLYGEKWSDGEASLALQFYCLYIIVLAMNGTSEA 465

Query: 363 FLHAVANESQLKKSNDSLLLFSLIYVMLNFLLIRSSGVVGLIFANSINMILRITYSAIFI 422
           FLHAV  +++L++SND LL+FSLIYV LN LLIRS+G +GLI ANS+NMI RI YS  FI
Sbjct: 466 FLHAVGTKNELERSNDMLLIFSLIYVALNILLIRSAGAIGLIMANSLNMIFRIIYSGRFI 525

Query: 423 KRYFK-NPPFSFSGCLPSGWIFLLFSGVLTLISERLFQDEQNFWSTFPLHFSIGLVCFLV 482
           +RYF+    FSF  C P GW  L+ S ++TLISE+   D +NFW TFPLHF IG++CF +
Sbjct: 526 QRYFQGTSSFSFRKCFPLGWQILILSSIITLISEKTILDRKNFWETFPLHFGIGVICFCL 585

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141197.11.1e-26798.96protein RFT1 homolog isoform X2 [Momordica charantia][more]
XP_023552765.11.0e-25494.17protein RFT1 homolog [Cucurbita pepo subsp. pepo][more]
XP_022922967.12.6e-25393.75protein RFT1 homolog [Cucurbita moschata] >KAG6576812.1 Protein RFT1-like protei... [more]
XP_038894185.13.4e-25394.15protein RFT1 homolog [Benincasa hispida][more]
XP_022985478.11.4e-25192.92protein RFT1 homolog [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q54IV77.0e-6031.42Protein RFT1 homolog OS=Dictyostelium discoideum OX=44689 GN=rft1 PE=3 SV=1[more]
Q0D2E81.2e-5933.93Protein RFT1 homolog OS=Xenopus tropicalis OX=8364 GN=rft1 PE=2 SV=1[more]
Q96AA36.3e-5332.02Protein RFT1 homolog OS=Homo sapiens OX=9606 GN=RFT1 PE=1 SV=1[more]
Q8C3B81.2e-5131.36Protein RFT1 homolog OS=Mus musculus OX=10090 GN=Rft1 PE=2 SV=2[more]
P382062.5e-3327.41Oligosaccharide translocation protein RFT1 OS=Saccharomyces cerevisiae (strain A... [more]
Match NameE-valueIdentityDescription
A0A6J1CJ715.2e-26898.96Protein RFT1 homolog OS=Momordica charantia OX=3673 GN=LOC111011652 PE=3 SV=1[more]
A0A6J1E4U91.2e-25393.75Protein RFT1 homolog OS=Cucurbita moschata OX=3662 GN=LOC111430792 PE=3 SV=1[more]
A0A6J1JDQ36.8e-25292.92Protein RFT1 homolog OS=Cucurbita maxima OX=3661 GN=LOC111483475 PE=3 SV=1[more]
A0A1S3BXG48.9e-25293.33Protein RFT1 homolog OS=Cucumis melo OX=3656 GN=LOC103494483 PE=3 SV=1[more]
A0A0A0KW956.4e-25092.71Protein RFT1 homolog OS=Cucumis sativus OX=3659 GN=Csa_4G022850 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G07630.14.6e-19269.44lipid transporters [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007594RFT1PFAMPF04506Rft-1coord: 2..481
e-value: 2.1E-129
score: 432.6
IPR007594RFT1PANTHERPTHR13117ENDOPLASMIC RETICULUM MULTISPAN TRANSMEMBRANE PROTEIN-RELATEDcoord: 2..481

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS000291.1MS000291.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006488 dolichol-linked oligosaccharide biosynthetic process
cellular_component GO:0016021 integral component of membrane