MELO3C020328 (gene) Melon (DHL92) v4

Overview
NameMELO3C020328
Typegene
OrganismCucumis melo cv. DHL92 (Melon (DHL92) v4)
Descriptionaspartic proteinase PCS1
Locationchr06: 13201237 .. 13202777 (+)
RNA-Seq ExpressionMELO3C020328
SyntenyMELO3C020328
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AACCCCCCACTTCACCAATCCCACCAAAACCCATCAAAAAAACCCCCACAATCAACCATGCTTCTAATCCTCTTCTCTCTCTCCTTATTCACTCTCCCCTTCTCCCAATCCAATTCCGTCTCTCTCCCCTTCCCACTTTCTCTCTCTGAAAAACCCTCCAATATTTCCCCAATATACGGTTCCCAGCTTTACGCCAAAAAGCCATCCTCCCATGGCTCCTTCAAGCTTCCTTTCAAATACTCCTCCACCGCCCTCGTCGTCTCTCTTCCGATCGGAACGCCGCCACAGCCCACTGACTTGGTTCTCGACACCGGCAGCCAACTCTCTTGGATTCAATGTCATGACAAGGTTAAGAAAAAATTGCCCCCCTTGCCCAAGCCTAAAACGGCCTCCTTTGATCCTTCTCTCTCCTCTTCTTTTTCTCTCCTCCCTTGTAATCACCCCATCTGCAAACCCCGAATTCCAGATTTTACCCTTCCTACTTCTTGTGACCAAAATCGCCTCTGCCACTACTCCTACTTCTACGCTGACGGTACCTTGGCCGAGGGAAATCTCGTCAGAGAGAAATTTAGCTTATCCAATTCCCTTTCTACCCCTCCCGTCATCCTCGGTTGCGCTCAAGCTTCCACCGAAAACAGGGGTATTTTGGGTATGAACAAGGGACGTTTGTCCTTTATCTCCCAAGCTAAAATCTCCAAATTCTCCTATTGCGTTCCCGCTCGAACCGGGTCTAATCCCACCGGGCTATTCTACCTCGGAGATAACCCCAATTCTTCCAGATTCAAGTACGTCACCATGTTGACTTTCCCCGAAAGTCAAAGCTCTCCGAATCTCGACCCACTGGCTTACACCCTCCCAATGAAGGGAATAAAAATAGCCGGAAAACGGCTCAACATCTCCCCAGCCGCTTTCAAACCGGACGCTGGTGGTTCGGGTCAAACGATGATTGACTCCGGTTCGGACCTCACTTATTTAGTGGATGAAGCGTATGAGAAGGTTAAAGAAGAGGTAGTGAGATTAGTGGGGGCGAAGATGAAGAAAGGCTACGTATACGCCGCCGTAGCCGACATGTGTTTCGACGCCCGCGTGACGGCGGAGGTGGGCCGCAGGATTGGCGGCATTTCGTTCGAGTTTGATAATGGGGTGGAGATTTTGGTGGGGAGAGGAGAAGGGGTTTTGACGGAAGTGGAAAAAGGGGTGAAGTGTGTGGGGTTCGGACGGTCAGAAAGGCTTGGGATTGGGAGTAATATAATCGGTACCGTTCATCAACAGAATATGTGGGTGGAGTATGATTTGACCAATAGGAGAATAGGGTTTGGTGGAGCTGAGTGTAGCAGATTGAAGTGATGATGGACGGTAAAGATTCATAAACGTGTGTGGTTTTTGGATGTTTATATAATCATATTTTATATTGTATCATGTGTACATGTGTATAGTTATTTCATTTCATATATATACTGGATATATAAATAAAACAAAATTTGTTCTATCATGTAAAATTGGTCACTATTAGCTAAGTTCATAAGGTGTGGATTATAATT

mRNA sequence

AACCCCCCACTTCACCAATCCCACCAAAACCCATCAAAAAAACCCCCACAATCAACCATGCTTCTAATCCTCTTCTCTCTCTCCTTATTCACTCTCCCCTTCTCCCAATCCAATTCCGTCTCTCTCCCCTTCCCACTTTCTCTCTCTGAAAAACCCTCCAATATTTCCCCAATATACGGTTCCCAGCTTTACGCCAAAAAGCCATCCTCCCATGGCTCCTTCAAGCTTCCTTTCAAATACTCCTCCACCGCCCTCGTCGTCTCTCTTCCGATCGGAACGCCGCCACAGCCCACTGACTTGGTTCTCGACACCGGCAGCCAACTCTCTTGGATTCAATGTCATGACAAGGTTAAGAAAAAATTGCCCCCCTTGCCCAAGCCTAAAACGGCCTCCTTTGATCCTTCTCTCTCCTCTTCTTTTTCTCTCCTCCCTTGTAATCACCCCATCTGCAAACCCCGAATTCCAGATTTTACCCTTCCTACTTCTTGTGACCAAAATCGCCTCTGCCACTACTCCTACTTCTACGCTGACGGTACCTTGGCCGAGGGAAATCTCGTCAGAGAGAAATTTAGCTTATCCAATTCCCTTTCTACCCCTCCCGTCATCCTCGGTTGCGCTCAAGCTTCCACCGAAAACAGGGGTATTTTGGGTATGAACAAGGGACGTTTGTCCTTTATCTCCCAAGCTAAAATCTCCAAATTCTCCTATTGCGTTCCCGCTCGAACCGGGTCTAATCCCACCGGGCTATTCTACCTCGGAGATAACCCCAATTCTTCCAGATTCAAGTACGTCACCATGTTGACTTTCCCCGAAAGTCAAAGCTCTCCGAATCTCGACCCACTGGCTTACACCCTCCCAATGAAGGGAATAAAAATAGCCGGAAAACGGCTCAACATCTCCCCAGCCGCTTTCAAACCGGACGCTGGTGGTTCGGGTCAAACGATGATTGACTCCGGTTCGGACCTCACTTATTTAGTGGATGAAGCGTATGAGAAGGTTAAAGAAGAGGTAGTGAGATTAGTGGGGGCGAAGATGAAGAAAGGCTACGTATACGCCGCCGTAGCCGACATGTGTTTCGACGCCCGCGTGACGGCGGAGGTGGGCCGCAGGATTGGCGGCATTTCGTTCGAGTTTGATAATGGGGTGGAGATTTTGGTGGGGAGAGGAGAAGGGGTTTTGACGGAAGTGGAAAAAGGGGTGAAGTGTGTGGGGTTCGGACGGTCAGAAAGGCTTGGGATTGGGAGTAATATAATCGGTACCGTTCATCAACAGAATATGTGGGTGGAGTATGATTTGACCAATAGGAGAATAGGGTTTGGTGGAGCTGAGTGTAGCAGATTGAAGTGATGATGGACGGTAAAGATTCATAAACGTGTGTGGTTTTTGGATGTTTATATAATCATATTTTATATTGTATCATGTGTACATGTGTATAGTTATTTCATTTCATATATATACTGGATATATAAATAAAACAAAATTTGTTCTATCATGTAAAATTGGTCACTATTAGCTAAGTTCATAAGGTGTGGATTATAATT

Coding sequence (CDS)

ATGCTTCTAATCCTCTTCTCTCTCTCCTTATTCACTCTCCCCTTCTCCCAATCCAATTCCGTCTCTCTCCCCTTCCCACTTTCTCTCTCTGAAAAACCCTCCAATATTTCCCCAATATACGGTTCCCAGCTTTACGCCAAAAAGCCATCCTCCCATGGCTCCTTCAAGCTTCCTTTCAAATACTCCTCCACCGCCCTCGTCGTCTCTCTTCCGATCGGAACGCCGCCACAGCCCACTGACTTGGTTCTCGACACCGGCAGCCAACTCTCTTGGATTCAATGTCATGACAAGGTTAAGAAAAAATTGCCCCCCTTGCCCAAGCCTAAAACGGCCTCCTTTGATCCTTCTCTCTCCTCTTCTTTTTCTCTCCTCCCTTGTAATCACCCCATCTGCAAACCCCGAATTCCAGATTTTACCCTTCCTACTTCTTGTGACCAAAATCGCCTCTGCCACTACTCCTACTTCTACGCTGACGGTACCTTGGCCGAGGGAAATCTCGTCAGAGAGAAATTTAGCTTATCCAATTCCCTTTCTACCCCTCCCGTCATCCTCGGTTGCGCTCAAGCTTCCACCGAAAACAGGGGTATTTTGGGTATGAACAAGGGACGTTTGTCCTTTATCTCCCAAGCTAAAATCTCCAAATTCTCCTATTGCGTTCCCGCTCGAACCGGGTCTAATCCCACCGGGCTATTCTACCTCGGAGATAACCCCAATTCTTCCAGATTCAAGTACGTCACCATGTTGACTTTCCCCGAAAGTCAAAGCTCTCCGAATCTCGACCCACTGGCTTACACCCTCCCAATGAAGGGAATAAAAATAGCCGGAAAACGGCTCAACATCTCCCCAGCCGCTTTCAAACCGGACGCTGGTGGTTCGGGTCAAACGATGATTGACTCCGGTTCGGACCTCACTTATTTAGTGGATGAAGCGTATGAGAAGGTTAAAGAAGAGGTAGTGAGATTAGTGGGGGCGAAGATGAAGAAAGGCTACGTATACGCCGCCGTAGCCGACATGTGTTTCGACGCCCGCGTGACGGCGGAGGTGGGCCGCAGGATTGGCGGCATTTCGTTCGAGTTTGATAATGGGGTGGAGATTTTGGTGGGGAGAGGAGAAGGGGTTTTGACGGAAGTGGAAAAAGGGGTGAAGTGTGTGGGGTTCGGACGGTCAGAAAGGCTTGGGATTGGGAGTAATATAATCGGTACCGTTCATCAACAGAATATGTGGGTGGAGTATGATTTGACCAATAGGAGAATAGGGTTTGGTGGAGCTGAGTGTAGCAGATTGAAGTGA

Protein sequence

MLLILFSLSLFTLPFSQSNSVSLPFPLSLSEKPSNISPIYGSQLYAKKPSSHGSFKLPFKYSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKVKKKLPPLPKPKTASFDPSLSSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFSLSNSLSTPPVILGCAQASTENRGILGMNKGRLSFISQAKISKFSYCVPARTGSNPTGLFYLGDNPNSSRFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKRLNISPAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDARVTAEVGRRIGGISFEFDNGVEILVGRGEGVLTEVEKGVKCVGFGRSERLGIGSNIIGTVHQQNMWVEYDLTNRRIGFGGAECSRLK
Homology
BLAST of MELO3C020328 vs. NCBI nr
Match: XP_008457122.1 (PREDICTED: aspartic proteinase PCS1 [Cucumis melo] >KAA0047379.1 aspartic proteinase PCS1 [Cucumis melo var. makuwa] >TYK14056.1 aspartic proteinase PCS1 [Cucumis melo var. makuwa])

HSP 1 Score: 861.7 bits (2225), Expect = 2.7e-246
Identity = 429/429 (100.00%), Postives = 429/429 (100.00%), Query Frame = 0

Query: 1   MLLILFSLSLFTLPFSQSNSVSLPFPLSLSEKPSNISPIYGSQLYAKKPSSHGSFKLPFK 60
           MLLILFSLSLFTLPFSQSNSVSLPFPLSLSEKPSNISPIYGSQLYAKKPSSHGSFKLPFK
Sbjct: 1   MLLILFSLSLFTLPFSQSNSVSLPFPLSLSEKPSNISPIYGSQLYAKKPSSHGSFKLPFK 60

Query: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKVKKKLPPLPKPKTASFDPSLSSS 120
           YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKVKKKLPPLPKPKTASFDPSLSSS
Sbjct: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKVKKKLPPLPKPKTASFDPSLSSS 120

Query: 121 FSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFSLSNSLSTP 180
           FSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFSLSNSLSTP
Sbjct: 121 FSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFSLSNSLSTP 180

Query: 181 PVILGCAQASTENRGILGMNKGRLSFISQAKISKFSYCVPARTGSNPTGLFYLGDNPNSS 240
           PVILGCAQASTENRGILGMNKGRLSFISQAKISKFSYCVPARTGSNPTGLFYLGDNPNSS
Sbjct: 181 PVILGCAQASTENRGILGMNKGRLSFISQAKISKFSYCVPARTGSNPTGLFYLGDNPNSS 240

Query: 241 RFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKRLNISPAAFKPDAGGSGQTMIDSG 300
           RFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKRLNISPAAFKPDAGGSGQTMIDSG
Sbjct: 241 RFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKRLNISPAAFKPDAGGSGQTMIDSG 300

Query: 301 SDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDARVTAEVGRRIGGISFEFD 360
           SDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDARVTAEVGRRIGGISFEFD
Sbjct: 301 SDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDARVTAEVGRRIGGISFEFD 360

Query: 361 NGVEILVGRGEGVLTEVEKGVKCVGFGRSERLGIGSNIIGTVHQQNMWVEYDLTNRRIGF 420
           NGVEILVGRGEGVLTEVEKGVKCVGFGRSERLGIGSNIIGTVHQQNMWVEYDLTNRRIGF
Sbjct: 361 NGVEILVGRGEGVLTEVEKGVKCVGFGRSERLGIGSNIIGTVHQQNMWVEYDLTNRRIGF 420

Query: 421 GGAECSRLK 430
           GGAECSRLK
Sbjct: 421 GGAECSRLK 429

BLAST of MELO3C020328 vs. NCBI nr
Match: XP_004140731.3 (aspartic proteinase PCS1 [Cucumis sativus] >KAE8650484.1 hypothetical protein Csa_011640 [Cucumis sativus])

HSP 1 Score: 811.6 bits (2095), Expect = 3.2e-231
Identity = 403/430 (93.72%), Postives = 414/430 (96.28%), Query Frame = 0

Query: 1   MLLILFSLSLFTLPFSQSNSVSLPFPLSLSEKPSNISPIYGSQLYAKKPSSHGSFKLPFK 60
           MLLILFSLSLFTL FSQSNS+SLPFPLSLSEKPSN  P Y SQLYAK+PSS+GSFKLPFK
Sbjct: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFK 60

Query: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHD-KVKKKLPPLPKPKTASFDPSLSS 120
           YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHD K+KK+LPPLPKPKTASFDPSLSS
Sbjct: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTASFDPSLSS 120

Query: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFSLSNSLST 180
           SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKF+ S SLST
Sbjct: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLST 180

Query: 181 PPVILGCAQASTENRGILGMNKGRLSFISQAKISKFSYCVPARTGSNPTGLFYLGDNPNS 240
           PPVILGCAQASTENRGILGMN+GRLSFISQAKISKFSYCVP+RTGSNPTGLFYLGDNPNS
Sbjct: 181 PPVILGCAQASTENRGILGMNRGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDNPNS 240

Query: 241 SRFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKRLNISPAAFKPDAGGSGQTMIDS 300
           S+FKYVTMLTFPESQSSPNLDPLAYTLPMK IKIAGKRLNI PAAFKPDAGGSGQTMIDS
Sbjct: 241 SKFKYVTMLTFPESQSSPNLDPLAYTLPMKAIKIAGKRLNIPPAAFKPDAGGSGQTMIDS 300

Query: 301 GSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDARVTAEVGRRIGGISFEF 360
           GSDLTYLVDEAYEKVKEEVVRLVGA MKKGYVYA VADMCFDA VTAEVGRRIGGISFEF
Sbjct: 301 GSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDAGVTAEVGRRIGGISFEF 360

Query: 361 DNGVEILVGRGEGVLTEVEKGVKCVGFGRSERLGIGSNIIGTVHQQNMWVEYDLTNRRIG 420
           DNGVEI VGRGEGVLTEVEKGVKCVG GRSERLGIGSNIIGTVHQQNMWVEYDL N+R+G
Sbjct: 361 DNGVEIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVG 420

Query: 421 FGGAECSRLK 430
           FGGAECSRLK
Sbjct: 421 FGGAECSRLK 430

BLAST of MELO3C020328 vs. NCBI nr
Match: XP_011651212.2 (aspartic proteinase PCS1 [Cucumis sativus] >KAE8650483.1 hypothetical protein Csa_010014 [Cucumis sativus])

HSP 1 Score: 800.0 bits (2065), Expect = 9.7e-228
Identity = 398/431 (92.34%), Postives = 411/431 (95.36%), Query Frame = 0

Query: 1   MLLILFSLSLFTLPFSQSNSVSLPFPLSLSEKPSNISPI-YGSQLYAKKPSSHGSFKLPF 60
           MLLILFSLSLFTL FSQSNS+SLPFPLSL+EKPSNI+P+ Y SQLY KKPSSHG FKLPF
Sbjct: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLTEKPSNITPLYYSSQLYVKKPSSHGPFKLPF 60

Query: 61  KYSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHD-KVKKKLPPLPKPKTASFDPSLS 120
           KYSS+ALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHD KVKK+LPPLPKPKTA+FDPSLS
Sbjct: 61  KYSSSALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTATFDPSLS 120

Query: 121 SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFSLSNSLS 180
           SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKF+ SNSLS
Sbjct: 121 SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLS 180

Query: 181 TPPVILGCAQASTENRGILGMNKGRLSFISQAKISKFSYCVPARTGSNPTGLFYLGDNPN 240
           TPPVILGCAQ STENRGILGMN GRLSFISQAKISKFSYCVP+RTG NPTGLFYLGDNPN
Sbjct: 181 TPPVILGCAQGSTENRGILGMNHGRLSFISQAKISKFSYCVPSRTGPNPTGLFYLGDNPN 240

Query: 241 SSRFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKRLNISPAAFKPDAGGSGQTMID 300
           SS+FKYVTMLTFPESQSSPNLDPLAYTLPMK IKIAGKRLNI PAAFKPDAGGSGQTMID
Sbjct: 241 SSKFKYVTMLTFPESQSSPNLDPLAYTLPMKAIKIAGKRLNIPPAAFKPDAGGSGQTMID 300

Query: 301 SGSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDARVTAEVGRRIGGISFE 360
           SGSDLTYLVDEAYEKVKEEVVRLVGA MKKGYVYAAVADMCFDA VT EVGRRIG +SFE
Sbjct: 301 SGSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYAAVADMCFDAGVTVEVGRRIGDMSFE 360

Query: 361 FDNGVEILVGRGEGVLTEVEKGVKCVGFGRSERLGIGSNIIGTVHQQNMWVEYDLTNRRI 420
           FDNGVEI VGRGEGVLTEVEKGVKCVG GRS RLGIGSNIIGTVHQQNMWVEYDL N+R+
Sbjct: 361 FDNGVEIFVGRGEGVLTEVEKGVKCVGIGRSGRLGIGSNIIGTVHQQNMWVEYDLANKRV 420

Query: 421 GFGGAECSRLK 430
           GFGGAECSRLK
Sbjct: 421 GFGGAECSRLK 431

BLAST of MELO3C020328 vs. NCBI nr
Match: XP_038878004.1 (aspartic proteinase PCS1 [Benincasa hispida])

HSP 1 Score: 739.2 bits (1907), Expect = 2.0e-209
Identity = 375/429 (87.41%), Postives = 394/429 (91.84%), Query Frame = 0

Query: 1   MLLILFSLSLFTLPFSQSNSVSLPFPLSLSEKPSNISPIYGSQLYAKKPSSHGSFKLPFK 60
           +L++ FSLSLF+L FSQSNS+SL FPLSLS+K SNISP+Y S LY+ K SSHGSFKLP K
Sbjct: 2   LLILFFSLSLFSLSFSQSNSLSLSFPLSLSKKTSNISPLYPS-LYS-KTSSHGSFKLPVK 61

Query: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKVKKKLPPLPKPKTASFDPSLSSS 120
           Y STALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKVKKK   LPKPKTASFDPSLSSS
Sbjct: 62  Y-STALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKVKKK---LPKPKTASFDPSLSSS 121

Query: 121 FSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFSLSNSLSTP 180
           FSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKF+ SNSL+TP
Sbjct: 122 FSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLTTP 181

Query: 181 PVILGCAQASTENRGILGMNKGRLSFISQAKISKFSYCVPARTGSNPTGLFYLGDNPNSS 240
           PVILGCAQASTENRGILGMN GRLSFISQAKISKFSYCVP RT  NPTGLFYLGDNPNSS
Sbjct: 182 PVILGCAQASTENRGILGMNTGRLSFISQAKISKFSYCVPRRTRFNPTGLFYLGDNPNSS 241

Query: 241 RFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKRLNISPAAFKPDAGGSGQTMIDSG 300
           +FKYVTMLTFP+SQ SPNLDPLAYTLPMKGIKIAGKRLNISPA FKPDA GSGQTMIDSG
Sbjct: 242 KFKYVTMLTFPKSQRSPNLDPLAYTLPMKGIKIAGKRLNISPAFFKPDASGSGQTMIDSG 301

Query: 301 SDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDARVTAEVGRRIGGISFEFD 360
           SDLTYL DEAYEKVKEE+VRLVG  MKKGY +AAVADMCF+A   A VGRRIG +SFEF+
Sbjct: 302 SDLTYLADEAYEKVKEEIVRLVGPMMKKGYEFAAVADMCFNAGEAAVVGRRIGNMSFEFE 361

Query: 361 NGVEILVGRGEGVLTEVEKGVKCVGFGRSERLGIGSNIIGTVHQQNMWVEYDLTNRRIGF 420
           NGVEILVG+GEGVL EVEKGVKCVG GRS RLGIGSNIIGTVHQQNMWVEYDL  +RIGF
Sbjct: 362 NGVEILVGKGEGVLAEVEKGVKCVGIGRSGRLGIGSNIIGTVHQQNMWVEYDLAKKRIGF 421

Query: 421 GGAECSRLK 430
           GGAECSRLK
Sbjct: 422 GGAECSRLK 424

BLAST of MELO3C020328 vs. NCBI nr
Match: XP_022983616.1 (aspartic proteinase PCS1-like [Cucurbita maxima])

HSP 1 Score: 692.2 bits (1785), Expect = 2.8e-195
Identity = 349/429 (81.35%), Postives = 379/429 (88.34%), Query Frame = 0

Query: 1   MLLILFSLSLFTLPFSQSNSVSLPFPLSLSEKPSNISPIYGSQLYAKKPSSHGSFKLPFK 60
           MLL LF +SL +L FS S+S+ L FPLSLS++PS +SP++ S       SS+GS KLPFK
Sbjct: 1   MLLSLFYISLLSLSFSHSHSLPLSFPLSLSKRPSPVSPLFSSL------SSYGSVKLPFK 60

Query: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKVKKKLPPLPKPKTASFDPSLSSS 120
           Y STALVVSLPIGTPPQPTDLVLDTGSQLSWIQCH K+ KK   L KPKTASFDPSLSSS
Sbjct: 61  Y-STALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHRKLHKK---LLKPKTASFDPSLSSS 120

Query: 121 FSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFSLSNSLSTP 180
           FSLLPCNHP+CKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKF+ SNS +TP
Sbjct: 121 FSLLPCNHPLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSRTTP 180

Query: 181 PVILGCAQASTENRGILGMNKGRLSFISQAKISKFSYCVPARTGSNPTGLFYLGDNPNSS 240
           PVILGCAQASTENRGILGMN GRLSF+SQAKISKFSYCVP RTG + TGLFYLGDNPNS+
Sbjct: 181 PVILGCAQASTENRGILGMNTGRLSFVSQAKISKFSYCVPGRTGPDSTGLFYLGDNPNSA 240

Query: 241 RFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKRLNISPAAFKPDAGGSGQTMIDSG 300
            FKY+++LTFP+SQ SPNLDPLAYTLP+KGIKIAG RLNIS A FKPD  GSGQTMIDSG
Sbjct: 241 NFKYISLLTFPKSQRSPNLDPLAYTLPLKGIKIAGNRLNISSAVFKPDRSGSGQTMIDSG 300

Query: 301 SDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDARVTAEVGRRIGGISFEFD 360
           SDLTYLVDEAYEKVKEE+V+LVG  MKKGY YAAVADMCF+   TAEVGRRI  +SFEF+
Sbjct: 301 SDLTYLVDEAYEKVKEEIVKLVGPLMKKGYEYAAVADMCFNDGETAEVGRRIRDMSFEFE 360

Query: 361 NGVEILVGRGEGVLTEVEKGVKCVGFGRSERLGIGSNIIGTVHQQNMWVEYDLTNRRIGF 420
           NGVEI VG+GEGVLTEVEKGVKCVGFGRS RLGI SNIIGTVHQ+N WVEYDL NRRIGF
Sbjct: 361 NGVEISVGKGEGVLTEVEKGVKCVGFGRSGRLGIASNIIGTVHQKNTWVEYDLANRRIGF 419

Query: 421 GGAECSRLK 430
           GGA+CSRLK
Sbjct: 421 GGADCSRLK 419

BLAST of MELO3C020328 vs. ExPASy Swiss-Prot
Match: Q9LZL3 (Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 5.4e-64
Identity = 160/445 (35.96%), Postives = 233/445 (52.36%), Query Frame = 0

Query: 6   FSLSLFTLPFSQSNSVSLPFPLSLSEKPSNISPIYGSQLYAKKPSSHGSFKLPFKYSSTA 65
           FS S F+   S S+S +L  PL     P++  P               + KL F ++ T 
Sbjct: 32  FSFSSFS---SSSSSQTLVLPLKTRITPTDHRP---------------TDKLHFHHNVT- 91

Query: 66  LVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKVKKKLPPLPKPKTASFDPSLSSSFSLLP 125
           L V+L +GTPPQ   +V+DTGS+LSW++C+          P P   +FDP+ SSS+S +P
Sbjct: 92  LTVTLTVGTPPQNISMVIDTGSELSWLRCNRSSN------PNP-VNNFDPTRSSSYSPIP 151

Query: 126 CNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFSLSNSLSTPPVILG 185
           C+ P C+ R  DF +P SCD ++LCH +  YAD + +EGNL  E F   NS +   +I G
Sbjct: 152 CSSPTCRTRTRDFLIPASCDSDKLCHATLSYADASSSEGNLAAEIFHFGNSTNDSNLIFG 211

Query: 186 C--------AQASTENRGILGMNKGRLSFISQAKISKFSYCVPARTGSNPTGLFYLGDNP 245
           C         +  T+  G+LGMN+G LSFISQ    KFSYC+ + T   P G   LGD  
Sbjct: 212 CMGSVSGSDPEEDTKTTGLLGMNRGSLSFISQMGFPKFSYCI-SGTDDFP-GFLLLGD-- 271

Query: 246 NSSRFKYVTMLTFPE----SQSSPNLDPLAYTLPMKGIKIAGKRLNISPAAFKPDAGGSG 305
             S F ++T L +      S   P  D +AYT+ + GIK+ GK L I  +   PD  G+G
Sbjct: 272 --SNFTWLTPLNYTPLIRISTPLPYFDRVAYTVQLTGIKVNGKLLPIPKSVLVPDHTGAG 331

Query: 306 QTMIDSGSDLTYLVDEAYEKVKEEVVR----LVGAKMKKGYVYAAVADMCF---DARVTA 365
           QTM+DSG+  T+L+   Y  ++   +     ++       +V+    D+C+     R+ +
Sbjct: 332 QTMVDSGTQFTFLLGPVYTALRSHFLNRTNGILTVYEDPDFVFQGTMDLCYRISPVRIRS 391

Query: 366 EVGRRIGGISFEFDNGVEILVGRGEGVLTEV------EKGVKCVGFGRSERLGIGSNIIG 425
            +  R+  +S  F+ G EI V  G+ +L  V         V C  FG S+ +G+ + +IG
Sbjct: 392 GILHRLPTVSLVFE-GAEIAVS-GQPLLYRVPHLTVGNDSVYCFTFGNSDLMGMEAYVIG 442

BLAST of MELO3C020328 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 1.9e-40
Identity = 115/365 (31.51%), Postives = 171/365 (46.85%), Query Frame = 0

Query: 67  VVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKVKKKLPPLPKPKTASFDPSLSSSFSLLPC 126
           +++L IGTP QP   ++DTGS L W QC     +         T  F+P  SSSFS LPC
Sbjct: 96  LMNLSIGTPAQPFSAIMDTGSDLIWTQC-----QPCTQCFNQSTPIFNPQGSSSFSTLPC 155

Query: 127 NHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFSLSNSLSTPPVILGC 186
           +  +C+       L +    N  C Y+Y Y DG+  +G++  E  +   S+S P +  GC
Sbjct: 156 SSQLCQ------ALSSPTCSNNFCQYTYGYGDGSETQGSMGTETLTF-GSVSIPNITFGC 215

Query: 187 AQ-----ASTENRGILGMNKGRLSFISQAKISKFSYCVPARTGSNPTGLFYLGDNPNSSR 246
            +           G++GM +G LS  SQ  ++KFSYC+     S P+ L  LG   NS  
Sbjct: 216 GENNQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLL-LGSLANSVT 275

Query: 247 FKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKRLNISPAAFKPDA-GGSGQTMIDSG 306
                      SQ      P  Y + + G+ +   RL I P+AF  ++  G+G  +IDSG
Sbjct: 276 AGSPNTTLIQSSQI-----PTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSG 335

Query: 307 SDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDARVTAEVGRRIGGISFEFD 366
           + LTY V+ AY+ V++E +  +   +  G   ++  D+CF    +     +I      FD
Sbjct: 336 TTLTYFVNNAYQSVRQEFISQINLPVVNG--SSSGFDLCFQT-PSDPSNLQIPTFVMHFD 395

Query: 367 NGVEILVGRGEGVLTEVEKGVKCVGFGRSERLGIGSNIIGTVHQQNMWVEYDLTNRRIGF 426
            G   L    E        G+ C+  G S +   G +I G + QQNM V YD  N  + F
Sbjct: 396 GG--DLELPSENYFISPSNGLICLAMGSSSQ---GMSIFGNIQQQNMLVVYDTGNSVVSF 434

BLAST of MELO3C020328 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 4.3e-37
Identity = 115/385 (29.87%), Postives = 173/385 (44.94%), Query Frame = 0

Query: 51  SHGSFKLPFKYSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKVKKKLPPLPKPKT 110
           S    + P        ++++ IGTP      ++DTGS L W QC    +    P P    
Sbjct: 81  SSSGIETPVYAGDGEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQPTP---- 140

Query: 111 ASFDPSLSSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREK 170
             F+P  SSSFS LPC    C+       LP+    N  C Y+Y Y DG+  +G +  E 
Sbjct: 141 -IFNPQDSSSFSTLPCESQYCQ------DLPSETCNNNECQYTYGYGDGSTTQGYMATET 200

Query: 171 FSLSNSLSTPPVILGCAQ-----ASTENRGILGMNKGRLSFISQAKISKFSYCVPARTGS 230
           F+   S S P +  GC +           G++GM  G LS  SQ  + +FSYC+ +   S
Sbjct: 201 FTFETS-SVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYGSS 260

Query: 231 NPTGLFYLGDNPNSSRFKYVTMLTFPESQSS-----PNLDPLAYTLPMKGIKIAGKRLNI 290
           +P+ L  LG   +            PE   S      +L+P  Y + ++GI + G  L I
Sbjct: 261 SPSTL-ALGSAASG----------VPEGSPSTTLIHSSLNPTYYYITLQGITVGGDNLGI 320

Query: 291 SPAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCF 350
             + F+    G+G  +IDSG+ LTYL  +AY  V +     +   +      ++    CF
Sbjct: 321 PSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQI--NLPTVDESSSGLSTCF 380

Query: 351 DARVTAEVGRRIGGISFEFDNGVEILVGRGEGVLTEVEKGVKCVGFGRSERLGIGSNIIG 410
                     ++  IS +FD GV + +G  + +L    +GV C+  G S +LGI  +I G
Sbjct: 381 QQPSDGST-VQVPEISMQFDGGV-LNLGE-QNILISPAEGVICLAMGSSSQLGI--SIFG 435

Query: 411 TVHQQNMWVEYDLTNRRIGFGGAEC 426
            + QQ   V YDL N  + F   +C
Sbjct: 441 NIQQQETQVLYDLQNLAVSFVPTQC 435

BLAST of MELO3C020328 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 7.1e-32
Identity = 100/362 (27.62%), Postives = 164/362 (45.30%), Query Frame = 0

Query: 72  IGTPPQPTDLVLDTGSQLSWIQCHDKVKKKLPPLPKPKTASFDPSLSSSFSLLPCNHPIC 131
           +GTP +   LVLDTGS ++WIQC     +      +     F+P+ SS++  L C+ P C
Sbjct: 168 VGTPAKEMYLVLDTGSDVNWIQC-----EPCADCYQQSDPVFNPTSSSTYKSLTCSAPQC 227

Query: 132 KPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFSLSNSLSTPPVILGCAQAS- 191
                   L TS  ++  C Y   Y DG+   G L  +  +  NS     V LGC   + 
Sbjct: 228 S------LLETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSGKINNVALGCGHDNE 287

Query: 192 ---TENRGILGMNKGRLSFISQAKISKFSYCVPARTGSNPTGLFYLGDNPNSSRFKYVTM 251
              T   G+LG+  G LS  +Q K + FSYC+  R            D+  SS   + ++
Sbjct: 288 GLFTGAAGLLGLGGGVLSITNQMKATSFSYCLVDR------------DSGKSSSLDFNSV 347

Query: 252 LTFPESQSSPNLD----PLAYTLPMKGIKIAGKRLNISPAAFKPDAGGSGQTMIDSGSDL 311
                  ++P L        Y + + G  + G+++ +  A F  DA GSG  ++D G+ +
Sbjct: 348 QLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAV 407

Query: 312 TYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDARVTAEVGRRIGGISFEFDNGV 371
           T L  +AY  +++  ++L    +KKG    ++ D C+D    + V  ++  ++F F  G 
Sbjct: 408 TRLQTQAYNSLRDAFLKLT-VNLKKGSSSISLFDTCYDFSSLSTV--KVPTVAFHFTGGK 467

Query: 372 EILVGRGEGVLTEVEKGVKCVGFGRSERLGIGSNIIGTVHQQNMWVEYDLTNRRIGFGGA 426
            + +     ++   + G  C  F  +       +IIG V QQ   + YDL+   IG  G 
Sbjct: 468 SLDLPAKNYLIPVDDSGTFCFAFAPTSS---SLSIIGNVQQQGTRITYDLSKNVIGLSGN 500

BLAST of MELO3C020328 vs. ExPASy Swiss-Prot
Match: Q9LEW3 (Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 2.3e-30
Identity = 117/406 (28.82%), Postives = 179/406 (44.09%), Query Frame = 0

Query: 34  SNISPIYG--SQLYAKKPSSHGSFKLPFKYSSTA----LVVSLPIGTPPQPTDLVLDTGS 93
           + +  IY   S+  A + S   S +LP K   T      +V++ IGTP     LV DTGS
Sbjct: 94  ARVESIYSKLSKNSANEVSEAKSTELPAKSGITLGSGNYIVTIGIGTPKHDLSLVFDTGS 153

Query: 94  QLSWIQCHDKVKKKLPPLPKPKTASFDPSLSSSFSLLPCNHPICKPRIPDFTLPTSCDQN 153
            L+W QC    +  L      K   F+PS SS++  + C+ P+C+          SC  +
Sbjct: 154 DLTWTQC----EPCLGSCYSQKEPKFNPSSSSTYQNVSCSSPMCED-------AESCSAS 213

Query: 154 RLCHYSYFYADGTLAEGNLVREKFSLSNSLSTPPVILGCAQAS----TENRGILGMNKGR 213
             C YS  Y D +  +G L +EKF+L+NS     V  GC + +        G+LG+  G+
Sbjct: 214 N-CVYSIVYGDKSFTQGFLAKEKFTLTNSDVLEDVYFGCGENNQGLFDGVAGLLGLGPGK 273

Query: 214 LSFISQAKISK---FSYCVPARTGSNPTGLFYLGDNPNSSRFKYVTMLTFPESQSSPNLD 273
           LS  +Q   +    FSYC+P+ T SN TG    G    S   K+  + +FP +       
Sbjct: 274 LSLPAQTTTTYNNIFSYCLPSFT-SNSTGHLTFGSAGISESVKFTPISSFPSA------- 333

Query: 274 PLAYTLPMKGIKIAGKRLNISPAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEV-V 333
              Y + + GI +  K L I+P +F  +       +IDSG+  T L  + Y +++     
Sbjct: 334 -FNYGIDIIGISVGDKELAITPNSFSTEG-----AIIDSGTVFTRLPTKVYAELRSVFKE 393

Query: 334 RLVGAKMKKGYVYAAVADMCFDARVTAEVGRRIGGISFEFDNGVEILVGRGEGVLTEVEK 393
           ++   K   GY    + D C+D      V       SF     VE+    G G+   ++ 
Sbjct: 394 KMSSYKSTSGY---GLFDTCYDFTGLDTVTYPTIAFSFAGSTVVEL---DGSGISLPIKI 453

Query: 394 GVKCVGFGRSERLGIGSNIIGTVHQQNMWVEYDLTNRRIGFGGAEC 426
              C+ F  ++ L     I G V Q  + V YD+   R+GF    C
Sbjct: 454 SQVCLAFAGNDDL---PAIFGNVQQTTLDVVYDVAGGRVGFAPNGC 464

BLAST of MELO3C020328 vs. ExPASy TrEMBL
Match: A0A5A7U1M2 (Aspartic proteinase PCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold363G00330 PE=3 SV=1)

HSP 1 Score: 861.7 bits (2225), Expect = 1.3e-246
Identity = 429/429 (100.00%), Postives = 429/429 (100.00%), Query Frame = 0

Query: 1   MLLILFSLSLFTLPFSQSNSVSLPFPLSLSEKPSNISPIYGSQLYAKKPSSHGSFKLPFK 60
           MLLILFSLSLFTLPFSQSNSVSLPFPLSLSEKPSNISPIYGSQLYAKKPSSHGSFKLPFK
Sbjct: 1   MLLILFSLSLFTLPFSQSNSVSLPFPLSLSEKPSNISPIYGSQLYAKKPSSHGSFKLPFK 60

Query: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKVKKKLPPLPKPKTASFDPSLSSS 120
           YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKVKKKLPPLPKPKTASFDPSLSSS
Sbjct: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKVKKKLPPLPKPKTASFDPSLSSS 120

Query: 121 FSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFSLSNSLSTP 180
           FSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFSLSNSLSTP
Sbjct: 121 FSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFSLSNSLSTP 180

Query: 181 PVILGCAQASTENRGILGMNKGRLSFISQAKISKFSYCVPARTGSNPTGLFYLGDNPNSS 240
           PVILGCAQASTENRGILGMNKGRLSFISQAKISKFSYCVPARTGSNPTGLFYLGDNPNSS
Sbjct: 181 PVILGCAQASTENRGILGMNKGRLSFISQAKISKFSYCVPARTGSNPTGLFYLGDNPNSS 240

Query: 241 RFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKRLNISPAAFKPDAGGSGQTMIDSG 300
           RFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKRLNISPAAFKPDAGGSGQTMIDSG
Sbjct: 241 RFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKRLNISPAAFKPDAGGSGQTMIDSG 300

Query: 301 SDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDARVTAEVGRRIGGISFEFD 360
           SDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDARVTAEVGRRIGGISFEFD
Sbjct: 301 SDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDARVTAEVGRRIGGISFEFD 360

Query: 361 NGVEILVGRGEGVLTEVEKGVKCVGFGRSERLGIGSNIIGTVHQQNMWVEYDLTNRRIGF 420
           NGVEILVGRGEGVLTEVEKGVKCVGFGRSERLGIGSNIIGTVHQQNMWVEYDLTNRRIGF
Sbjct: 361 NGVEILVGRGEGVLTEVEKGVKCVGFGRSERLGIGSNIIGTVHQQNMWVEYDLTNRRIGF 420

Query: 421 GGAECSRLK 430
           GGAECSRLK
Sbjct: 421 GGAECSRLK 429

BLAST of MELO3C020328 vs. ExPASy TrEMBL
Match: A0A1S3C4D2 (aspartic proteinase PCS1 OS=Cucumis melo OX=3656 GN=LOC103496869 PE=3 SV=1)

HSP 1 Score: 861.7 bits (2225), Expect = 1.3e-246
Identity = 429/429 (100.00%), Postives = 429/429 (100.00%), Query Frame = 0

Query: 1   MLLILFSLSLFTLPFSQSNSVSLPFPLSLSEKPSNISPIYGSQLYAKKPSSHGSFKLPFK 60
           MLLILFSLSLFTLPFSQSNSVSLPFPLSLSEKPSNISPIYGSQLYAKKPSSHGSFKLPFK
Sbjct: 1   MLLILFSLSLFTLPFSQSNSVSLPFPLSLSEKPSNISPIYGSQLYAKKPSSHGSFKLPFK 60

Query: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKVKKKLPPLPKPKTASFDPSLSSS 120
           YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKVKKKLPPLPKPKTASFDPSLSSS
Sbjct: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKVKKKLPPLPKPKTASFDPSLSSS 120

Query: 121 FSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFSLSNSLSTP 180
           FSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFSLSNSLSTP
Sbjct: 121 FSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFSLSNSLSTP 180

Query: 181 PVILGCAQASTENRGILGMNKGRLSFISQAKISKFSYCVPARTGSNPTGLFYLGDNPNSS 240
           PVILGCAQASTENRGILGMNKGRLSFISQAKISKFSYCVPARTGSNPTGLFYLGDNPNSS
Sbjct: 181 PVILGCAQASTENRGILGMNKGRLSFISQAKISKFSYCVPARTGSNPTGLFYLGDNPNSS 240

Query: 241 RFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKRLNISPAAFKPDAGGSGQTMIDSG 300
           RFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKRLNISPAAFKPDAGGSGQTMIDSG
Sbjct: 241 RFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKRLNISPAAFKPDAGGSGQTMIDSG 300

Query: 301 SDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDARVTAEVGRRIGGISFEFD 360
           SDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDARVTAEVGRRIGGISFEFD
Sbjct: 301 SDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDARVTAEVGRRIGGISFEFD 360

Query: 361 NGVEILVGRGEGVLTEVEKGVKCVGFGRSERLGIGSNIIGTVHQQNMWVEYDLTNRRIGF 420
           NGVEILVGRGEGVLTEVEKGVKCVGFGRSERLGIGSNIIGTVHQQNMWVEYDLTNRRIGF
Sbjct: 361 NGVEILVGRGEGVLTEVEKGVKCVGFGRSERLGIGSNIIGTVHQQNMWVEYDLTNRRIGF 420

Query: 421 GGAECSRLK 430
           GGAECSRLK
Sbjct: 421 GGAECSRLK 429

BLAST of MELO3C020328 vs. ExPASy TrEMBL
Match: A0A6J1J2U3 (aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111482170 PE=3 SV=1)

HSP 1 Score: 692.2 bits (1785), Expect = 1.4e-195
Identity = 349/429 (81.35%), Postives = 379/429 (88.34%), Query Frame = 0

Query: 1   MLLILFSLSLFTLPFSQSNSVSLPFPLSLSEKPSNISPIYGSQLYAKKPSSHGSFKLPFK 60
           MLL LF +SL +L FS S+S+ L FPLSLS++PS +SP++ S       SS+GS KLPFK
Sbjct: 1   MLLSLFYISLLSLSFSHSHSLPLSFPLSLSKRPSPVSPLFSSL------SSYGSVKLPFK 60

Query: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKVKKKLPPLPKPKTASFDPSLSSS 120
           Y STALVVSLPIGTPPQPTDLVLDTGSQLSWIQCH K+ KK   L KPKTASFDPSLSSS
Sbjct: 61  Y-STALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHRKLHKK---LLKPKTASFDPSLSSS 120

Query: 121 FSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFSLSNSLSTP 180
           FSLLPCNHP+CKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKF+ SNS +TP
Sbjct: 121 FSLLPCNHPLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSRTTP 180

Query: 181 PVILGCAQASTENRGILGMNKGRLSFISQAKISKFSYCVPARTGSNPTGLFYLGDNPNSS 240
           PVILGCAQASTENRGILGMN GRLSF+SQAKISKFSYCVP RTG + TGLFYLGDNPNS+
Sbjct: 181 PVILGCAQASTENRGILGMNTGRLSFVSQAKISKFSYCVPGRTGPDSTGLFYLGDNPNSA 240

Query: 241 RFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKRLNISPAAFKPDAGGSGQTMIDSG 300
            FKY+++LTFP+SQ SPNLDPLAYTLP+KGIKIAG RLNIS A FKPD  GSGQTMIDSG
Sbjct: 241 NFKYISLLTFPKSQRSPNLDPLAYTLPLKGIKIAGNRLNISSAVFKPDRSGSGQTMIDSG 300

Query: 301 SDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDARVTAEVGRRIGGISFEFD 360
           SDLTYLVDEAYEKVKEE+V+LVG  MKKGY YAAVADMCF+   TAEVGRRI  +SFEF+
Sbjct: 301 SDLTYLVDEAYEKVKEEIVKLVGPLMKKGYEYAAVADMCFNDGETAEVGRRIRDMSFEFE 360

Query: 361 NGVEILVGRGEGVLTEVEKGVKCVGFGRSERLGIGSNIIGTVHQQNMWVEYDLTNRRIGF 420
           NGVEI VG+GEGVLTEVEKGVKCVGFGRS RLGI SNIIGTVHQ+N WVEYDL NRRIGF
Sbjct: 361 NGVEISVGKGEGVLTEVEKGVKCVGFGRSGRLGIASNIIGTVHQKNTWVEYDLANRRIGF 419

Query: 421 GGAECSRLK 430
           GGA+CSRLK
Sbjct: 421 GGADCSRLK 419

BLAST of MELO3C020328 vs. ExPASy TrEMBL
Match: A0A6J1F8Z2 (aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111441919 PE=3 SV=1)

HSP 1 Score: 688.3 bits (1775), Expect = 2.0e-194
Identity = 349/433 (80.60%), Postives = 380/433 (87.76%), Query Frame = 0

Query: 1   MLLILFSLSLFTLPFSQSNSVSLPFPLSLSEKP----SNISPIYGSQLYAKKPSSHGSFK 60
           MLL LF LSL +L FS S+S+SL FPLSLS++P    S++SP++ S       S++GS K
Sbjct: 1   MLLSLFYLSLLSLSFSHSHSLSLSFPLSLSKRPSPVSSSVSPLFSSL------SAYGSVK 60

Query: 61  LPFKYSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKVKKKLPPLPKPKTASFDPS 120
           LPFKY STALVVSLPIGTPPQPTDLVLDTGSQLSWIQCH KV KK   L KPKT SFDPS
Sbjct: 61  LPFKY-STALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHRKVHKK---LLKPKTTSFDPS 120

Query: 121 LSSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFSLSNS 180
           LSSSFSLLPCNHP+CKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKF+ SNS
Sbjct: 121 LSSSFSLLPCNHPLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNS 180

Query: 181 LSTPPVILGCAQASTENRGILGMNKGRLSFISQAKISKFSYCVPARTGSNPTGLFYLGDN 240
            +TPPVILGCAQASTENRGILGMN GRLSF+SQAKISKFSYCVP RTG + TGLFYLGDN
Sbjct: 181 RTTPPVILGCAQASTENRGILGMNTGRLSFVSQAKISKFSYCVPGRTGPDSTGLFYLGDN 240

Query: 241 PNSSRFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKRLNISPAAFKPDAGGSGQTM 300
           PNS+ FKY+++LTFP+SQ SPNLDPLAYTLP+KGIKIAG RLNIS A FKPD  GSGQTM
Sbjct: 241 PNSANFKYISLLTFPKSQRSPNLDPLAYTLPLKGIKIAGNRLNISSAVFKPDRSGSGQTM 300

Query: 301 IDSGSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDARVTAEVGRRIGGIS 360
           IDSGSDLTYLVDEAYEKVKEE+V+LVG  MKKGY YAAVADMCF+   TAEVGRRI  +S
Sbjct: 301 IDSGSDLTYLVDEAYEKVKEEIVKLVGPLMKKGYEYAAVADMCFNDGETAEVGRRIRDMS 360

Query: 361 FEFDNGVEILVGRGEGVLTEVEKGVKCVGFGRSERLGIGSNIIGTVHQQNMWVEYDLTNR 420
           FEF+NGVEI VG+GEGVLTEVEKGVKCVGFGRS RLGI SNIIGTVHQ+N W+EYDL NR
Sbjct: 361 FEFENGVEISVGKGEGVLTEVEKGVKCVGFGRSGRLGIASNIIGTVHQKNTWIEYDLANR 420

Query: 421 RIGFGGAECSRLK 430
           RIGFGGA+CSRLK
Sbjct: 421 RIGFGGADCSRLK 423

BLAST of MELO3C020328 vs. ExPASy TrEMBL
Match: A0A0A0L6V5 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G188350 PE=3 SV=1)

HSP 1 Score: 604.7 bits (1558), Expect = 2.9e-169
Identity = 321/430 (74.65%), Postives = 328/430 (76.28%), Query Frame = 0

Query: 1   MLLILFSLSLFTLPFSQSNSVSLPFPLSLSEKPSNISPIYGSQLYAKKPSSHGSFKLPFK 60
           MLLILFSLSLFTL FSQSNS+SLPFPLSLSEKPSN  P Y SQLYAK+PSS+GSFKLPFK
Sbjct: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFK 60

Query: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHD-KVKKKLPPLPKPKTASFDPSLSS 120
           YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHD KVKK+LPPLPKPKTASFDPSLSS
Sbjct: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLSS 120

Query: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFSLSNSLST 180
           SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKF+ S SLST
Sbjct: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLST 180

Query: 181 PPVILGCAQASTENRGILGMNKGRLSFISQAKISKFSYCVPARTGSNPTGLFYLGDNPNS 240
           PPVILGCAQASTENR                                             
Sbjct: 181 PPVILGCAQASTENR--------------------------------------------- 240

Query: 241 SRFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKRLNISPAAFKPDAGGSGQTMIDS 300
                                                      AAFKPDAGGSGQTMIDS
Sbjct: 241 -------------------------------------------AAFKPDAGGSGQTMIDS 300

Query: 301 GSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDARVTAEVGRRIGGISFEF 360
           GSDLTYLVDEAYEKVKEEVVRLVGA MKKGYVYA VADMCFDA VTAEVGRRIGGISFEF
Sbjct: 301 GSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDAGVTAEVGRRIGGISFEF 342

Query: 361 DNGVEILVGRGEGVLTEVEKGVKCVGFGRSERLGIGSNIIGTVHQQNMWVEYDLTNRRIG 420
           DNGVEI VGRGEGVLTEVEKGVKCVG GRSERLGIGSNIIGTVHQQNMWVEYDL N+R+G
Sbjct: 361 DNGVEIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVG 342

Query: 421 FGGAECSRLK 430
           FGGAECSRLK
Sbjct: 421 FGGAECSRLK 342

BLAST of MELO3C020328 vs. TAIR 10
Match: AT5G37540.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 545.4 bits (1404), Expect = 4.0e-155
Identity = 280/435 (64.37%), Postives = 337/435 (77.47%), Query Frame = 0

Query: 2   LLILFSLSLFTLPFSQSNSVSLPFPL-SLSEKPSNISPIYGSQLYAKK----PSSHGSFK 61
           LL +F    +++  S S+S+SL FPL SL   P+  S  + + L +++    PSS  +F+
Sbjct: 12  LLYIFFFFCYSVSLSWSSSLSLHFPLTSLRLTPTTNSSSFKTSLLSRRNPSPPSSPYTFR 71

Query: 62  LPFKYSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKVKKKLPPLPKPKTASFDPS 121
              KY S AL++SLPIGTP Q  +LVLDTGSQLSWIQCH K  KK  PLP P T SFDPS
Sbjct: 72  SNIKY-SMALILSLPIGTPSQSQELVLDTGSQLSWIQCHPKKIKK--PLP-PPTTSFDPS 131

Query: 122 LSSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFSLSNS 181
           LSSSFS LPC+HP+CKPRIPDFTLPTSCD NRLCHYSYFYADGT AEGNLV+EKF+ SNS
Sbjct: 132 LSSSFSDLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKFTFSNS 191

Query: 182 LSTPPVILGCAQASTENRGILGMNKGRLSFISQAKISKFSYCVPART---GSNPTGLFYL 241
            +TPP+ILGCA+ ST+ +GILGMN GRLSFISQAKISKFSYC+P R+   G   TG FYL
Sbjct: 192 QTTPPLILGCAKESTDEKGILGMNLGRLSFISQAKISKFSYCIPTRSNRPGLASTGSFYL 251

Query: 242 GDNPNSSRFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKRLNISPAAFKPDAGGSG 301
           GDNPNS  FKYV++LTFP+SQ  PNLDPLAYT+P++GI+I  KRLNI  + F+PDAGGSG
Sbjct: 252 GDNPNSRGFKYVSLLTFPQSQRMPNLDPLAYTVPLQGIRIGQKRLNIPGSVFRPDAGGSG 311

Query: 302 QTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDARVTAEVGRRIG 361
           QTM+DSGS+ T+LVD AY+KVKEE+VRLVG+++KKGYVY + ADMCFD   + E+GR IG
Sbjct: 312 QTMVDSGSEFTHLVDVAYDKVKEEIVRLVGSRLKKGYVYGSTADMCFDGNHSMEIGRLIG 371

Query: 362 GISFEFDNGVEILVGRGEGVLTEVEKGVKCVGFGRSERLGIGSNIIGTVHQQNMWVEYDL 421
            + FEF  GVEILV + + +L  V  G+ CVG GRS  LG  SNIIG VHQQN+WVE+D+
Sbjct: 372 DLVFEFGRGVEILVEK-QSLLVNVGGGIHCVGIGRSSMLGAASNIIGNVHQQNLWVEFDV 431

Query: 422 TNRRIGFGGAECSRL 429
           TNRR+GF  AEC  L
Sbjct: 432 TNRRVGFSKAECRLL 441

BLAST of MELO3C020328 vs. TAIR 10
Match: AT1G66180.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 523.5 bits (1347), Expect = 1.6e-148
Identity = 272/421 (64.61%), Postives = 321/421 (76.25%), Query Frame = 0

Query: 16  SQSNSVSLPFPL-SLSEKPSNISPIYGSQLYAKKPSSHGS----FKLPFKYSSTALVVSL 75
           S S S+SL  PL SL    +  S  + + L ++K  S  S    F+  FKY S AL++SL
Sbjct: 18  SLSTSLSLHLPLTSLPISTTTNSHRFTTSLLSRKNPSPSSPPYNFRSRFKY-SMALIISL 77

Query: 76  PIGTPPQPTDLVLDTGSQLSWIQCHDKVKKKLPPLPKPKTASFDPSLSSSFSLLPCNHPI 135
           PIGTPPQ   +VLDTGSQLSWIQCH   +KKLP  PKPKT SFDPSLSSSFS LPC+HP+
Sbjct: 78  PIGTPPQAQQMVLDTGSQLSWIQCH---RKKLP--PKPKT-SFDPSLSSSFSTLPCSHPL 137

Query: 136 CKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFSLSNSLSTPPVILGCAQAS 195
           CKPRIPDFTLPTSCD NRLCHYSYFYADGT AEGNLV+EK + SN+  TPP+ILGCA  S
Sbjct: 138 CKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKITFSNTEITPPLILGCATES 197

Query: 196 TENRGILGMNKGRLSFISQAKISKFSYCVPA---RTGSNPTGLFYLGDNPNSSRFKYVTM 255
           +++RGILGMN+GRLSF+SQAKISKFSYC+P    R G  PTG FYLGDNPNS  FKYV++
Sbjct: 198 SDDRGILGMNRGRLSFVSQAKISKFSYCIPPKSNRPGFTPTGSFYLGDNPNSHGFKYVSL 257

Query: 256 LTFPESQSSPNLDPLAYTLPMKGIKIAGKRLNISPAAFKPDAGGSGQTMIDSGSDLTYLV 315
           LTFPESQ  PNLDPLAYT+PM GI+   K+LNIS + F+PDAGGSGQTM+DSGS+ T+LV
Sbjct: 258 LTFPESQRMPNLDPLAYTVPMIGIRFGLKKLNISGSVFRPDAGGSGQTMVDSGSEFTHLV 317

Query: 316 DEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDARVTAEVGRRIGGISFEFDNGVEILV 375
           D AY+KV+ E++  VG ++KKGYVY   ADMCFD  V A + R IG + F F  GVEILV
Sbjct: 318 DAAYDKVRAEIMTRVGRRLKKGYVYGGTADMCFDGNV-AMIPRLIGDLVFVFTRGVEILV 377

Query: 376 GRGEGVLTEVEKGVKCVGFGRSERLGIGSNIIGTVHQQNMWVEYDLTNRRIGFGGAECSR 429
            + E VL  V  G+ CVG GRS  LG  SNIIG VHQQN+WVE+D+TNRR+GF  A+CSR
Sbjct: 378 PK-ERVLVNVGGGIHCVGIGRSSMLGAASNIIGNVHQQNLWVEFDVTNRRVGFAKADCSR 429

BLAST of MELO3C020328 vs. TAIR 10
Match: AT5G02190.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 246.5 bits (628), Expect = 3.8e-65
Identity = 160/445 (35.96%), Postives = 233/445 (52.36%), Query Frame = 0

Query: 6   FSLSLFTLPFSQSNSVSLPFPLSLSEKPSNISPIYGSQLYAKKPSSHGSFKLPFKYSSTA 65
           FS S F+   S S+S +L  PL     P++  P               + KL F ++ T 
Sbjct: 32  FSFSSFS---SSSSSQTLVLPLKTRITPTDHRP---------------TDKLHFHHNVT- 91

Query: 66  LVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKVKKKLPPLPKPKTASFDPSLSSSFSLLP 125
           L V+L +GTPPQ   +V+DTGS+LSW++C+          P P   +FDP+ SSS+S +P
Sbjct: 92  LTVTLTVGTPPQNISMVIDTGSELSWLRCNRSSN------PNP-VNNFDPTRSSSYSPIP 151

Query: 126 CNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFSLSNSLSTPPVILG 185
           C+ P C+ R  DF +P SCD ++LCH +  YAD + +EGNL  E F   NS +   +I G
Sbjct: 152 CSSPTCRTRTRDFLIPASCDSDKLCHATLSYADASSSEGNLAAEIFHFGNSTNDSNLIFG 211

Query: 186 C--------AQASTENRGILGMNKGRLSFISQAKISKFSYCVPARTGSNPTGLFYLGDNP 245
           C         +  T+  G+LGMN+G LSFISQ    KFSYC+ + T   P G   LGD  
Sbjct: 212 CMGSVSGSDPEEDTKTTGLLGMNRGSLSFISQMGFPKFSYCI-SGTDDFP-GFLLLGD-- 271

Query: 246 NSSRFKYVTMLTFPE----SQSSPNLDPLAYTLPMKGIKIAGKRLNISPAAFKPDAGGSG 305
             S F ++T L +      S   P  D +AYT+ + GIK+ GK L I  +   PD  G+G
Sbjct: 272 --SNFTWLTPLNYTPLIRISTPLPYFDRVAYTVQLTGIKVNGKLLPIPKSVLVPDHTGAG 331

Query: 306 QTMIDSGSDLTYLVDEAYEKVKEEVVR----LVGAKMKKGYVYAAVADMCF---DARVTA 365
           QTM+DSG+  T+L+   Y  ++   +     ++       +V+    D+C+     R+ +
Sbjct: 332 QTMVDSGTQFTFLLGPVYTALRSHFLNRTNGILTVYEDPDFVFQGTMDLCYRISPVRIRS 391

Query: 366 EVGRRIGGISFEFDNGVEILVGRGEGVLTEV------EKGVKCVGFGRSERLGIGSNIIG 425
            +  R+  +S  F+ G EI V  G+ +L  V         V C  FG S+ +G+ + +IG
Sbjct: 392 GILHRLPTVSLVFE-GAEIAVS-GQPLLYRVPHLTVGNDSVYCFTFGNSDLMGMEAYVIG 442

BLAST of MELO3C020328 vs. TAIR 10
Match: AT2G39710.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 239.2 bits (609), Expect = 6.1e-63
Identity = 155/437 (35.47%), Postives = 238/437 (54.46%), Query Frame = 0

Query: 7   SLSLFTLPFSQSNSVSLPFPLSLSEKPSNISPIYGSQLYAKKPSSHGSFKLPFKYSSTAL 66
           SLSL +  F + + + L FPL+  +  S    +  S    K P S  S KL F+++ T L
Sbjct: 9   SLSL-SKNFLRISVLLLIFPLTFCKTSSTNQTLLFSLKTQKLPQS-SSDKLSFRHNVT-L 68

Query: 67  VVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKVKKKLPPLPKPKTASFDPSLSSSFSLLPC 126
            V+L +G PPQ   +VLDTGS+LSW+ C     KK P L     + F+P  SS++S +PC
Sbjct: 69  TVTLAVGDPPQNISMVLDTGSELSWLHC-----KKSPNL----GSVFNPVSSSTYSPVPC 128

Query: 127 NHPICKPRIPDFTLPTSCD-QNRLCHYSYFYADGTLAEGNLVREKFSLSNSLSTPPVILG 186
           + PIC+ R  D  +P SCD +  LCH +  YAD T  EGNL  E F +  S++ P  + G
Sbjct: 129 SSPICRTRTRDLPIPASCDPKTHLCHVAISYADATSIEGNLAHETFVI-GSVTRPGTLFG 188

Query: 187 C--------AQASTENRGILGMNKGRLSFISQAKISKFSYCVPARTGSNPTGLFYLGDNP 246
           C        ++   ++ G++GMN+G LSF++Q   SKFSYC+   +GS+ +G   LGD  
Sbjct: 189 CMDSGLSSNSEEDAKSTGLMGMNRGSLSFVNQLGFSKFSYCI---SGSDSSGFLLLGDAS 248

Query: 247 NS--SRFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKRLNISPAAFKPDAGGSGQT 306
            S     +Y  ++   +S   P  D +AYT+ ++GI++  K L++  + F PD  G+GQT
Sbjct: 249 YSWLGPIQYTPLVL--QSTPLPYFDRVAYTVQLEGIRVGSKILSLPKSVFVPDHTGAGQT 308

Query: 307 MIDSGSDLTYLVDEAYEKVKEEVVRLVGAKMK----KGYVYAAVADMCFDARVTAE---V 366
           M+DSG+  T+L+   Y  +K E +    + ++      +V+    D+C+    T      
Sbjct: 309 MVDSGTQFTFLMGPVYTALKNEFITQTKSVLRLVDDPDFVFQGTMDLCYKVGSTTRPNFS 368

Query: 367 GRRIGGISF---EFDNGVEILVGRGEGVLTEVEKGVKCVGFGRSERLGIGSNIIGTVHQQ 423
           G  +  + F   E     + L+ R  G  +E ++ V C  FG S+ LGI + +IG  HQQ
Sbjct: 369 GLPMVSLMFRGAEMSVSGQKLLYRVNGAGSEGKEEVYCFTFGNSDLLGIEAFVIGHHHQQ 427

BLAST of MELO3C020328 vs. TAIR 10
Match: AT2G03200.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 152.9 bits (385), Expect = 5.8e-37
Identity = 115/397 (28.97%), Postives = 180/397 (45.34%), Query Frame = 0

Query: 46  AKKPSSHGSFKLPFKYSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKVKKKLPPL 105
           A KP    + K P    S   ++ L IG P      ++DTGS L W QC    +    P 
Sbjct: 87  ASKPDDTNNIKAPTHGGSGEFLMELSIGNPAVKYSAIVDTGSDLIWTQCKPCTECFDQPT 146

Query: 106 PKPKTASFDPSLSSSFSLLPCNHPICKPRIPDFTLPTS-CDQNR-LCHYSYFYADGTLAE 165
           P      FDP  SSS+S + C+  +C        LP S C++++  C Y Y Y D +   
Sbjct: 147 P-----IFDPEKSSSYSKVGCSSGLCN------ALPRSNCNEDKDACEYLYTYGDYSSTR 206

Query: 166 GNLVREKFSLSNSLSTPPVILGC-----AQASTENRGILGMNKGRLSFISQAKISKFSYC 225
           G L  E F+  +  S   +  GC         ++  G++G+ +G LS ISQ K +KFSYC
Sbjct: 207 GLLATETFTFEDENSISGIGFGCGVENEGDGFSQGSGLVGLGRGPLSLISQLKETKFSYC 266

Query: 226 VPARTGSNPTGLFYLGDNPNSSRFKYVTMLTFPESQSSPNL----DPLAYTLPMKGIKIA 285
           + +   S  +   ++G   +    K    L    +++   L     P  Y L ++GI + 
Sbjct: 267 LTSIEDSEASSSLFIGSLASGIVNKTGASLDGEVTKTMSLLRNPDQPSFYYLELQGITVG 326

Query: 286 GKRLNISPAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAA 345
            KRL++  + F+    G+G  +IDSG+ +TYL + A++ +KEE    +   +      + 
Sbjct: 327 AKRLSVEKSTFELAEDGTGGMIIDSGTTITYLEETAFKVLKEEFTSRMSLPVDDS--GST 386

Query: 346 VADMCF---DARVTAEVGRRIGGISFEFDNGVEILVGRGEGVLTEVEKGVKCVGFGRSER 405
             D+CF   DA     V + I    F F  G ++ +     ++ +   GV C+  G S  
Sbjct: 387 GLDLCFKLPDAAKNIAVPKMI----FHF-KGADLELPGENYMVADSSTGVLCLAMGSSN- 446

Query: 406 LGIGSNIIGTVHQQNMWVEYDLTNRRIGFGGAECSRL 429
              G +I G V QQN  V +DL    + F   EC +L
Sbjct: 447 ---GMSIFGNVQQQNFNVLHDLEKETVSFVPTECGKL 461

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008457122.12.7e-246100.00PREDICTED: aspartic proteinase PCS1 [Cucumis melo] >KAA0047379.1 aspartic protei... [more]
XP_004140731.33.2e-23193.72aspartic proteinase PCS1 [Cucumis sativus] >KAE8650484.1 hypothetical protein Cs... [more]
XP_011651212.29.7e-22892.34aspartic proteinase PCS1 [Cucumis sativus] >KAE8650483.1 hypothetical protein Cs... [more]
XP_038878004.12.0e-20987.41aspartic proteinase PCS1 [Benincasa hispida][more]
XP_022983616.12.8e-19581.35aspartic proteinase PCS1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9LZL35.4e-6435.96Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1[more]
Q766C31.9e-4031.51Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Q766C24.3e-3729.87Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
Q9LS407.1e-3227.62Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LEW32.3e-3028.82Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7U1M21.3e-246100.00Aspartic proteinase PCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S3C4D21.3e-246100.00aspartic proteinase PCS1 OS=Cucumis melo OX=3656 GN=LOC103496869 PE=3 SV=1[more]
A0A6J1J2U31.4e-19581.35aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111482170 PE=3 S... [more]
A0A6J1F8Z22.0e-19480.60aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111441919 PE=3... [more]
A0A0A0L6V52.9e-16974.65Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G18835... [more]
Match NameE-valueIdentityDescription
AT5G37540.14.0e-15564.37Eukaryotic aspartyl protease family protein [more]
AT1G66180.11.6e-14864.61Eukaryotic aspartyl protease family protein [more]
AT5G02190.13.8e-6535.96Eukaryotic aspartyl protease family protein [more]
AT2G39710.16.1e-6335.47Eukaryotic aspartyl protease family protein [more]
AT2G03200.15.8e-3728.97Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPRINTSPR00792PEPSINcoord: 397..412
score: 25.04
coord: 72..92
score: 47.5
coord: 295..306
score: 28.66
IPR001461Aspartic peptidase A1 familyPANTHERPTHR47965ASPARTYL PROTEASE-RELATEDcoord: 2..428
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 263..420
e-value: 9.9E-33
score: 113.2
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 45..233
e-value: 9.6E-36
score: 125.5
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 234..429
e-value: 1.0E-38
score: 134.8
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 63..427
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 68..235
e-value: 1.5E-35
score: 123.0
NoneNo IPR availablePANTHERPTHR47965:SF51EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEINcoord: 2..428
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 81..92
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 66..421
score: 32.542595
IPR034161Pepsin-like domain, plantCDDcd05476pepsin_A_like_plantcoord: 67..425
e-value: 2.78237E-70
score: 220.983

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C020328.1MELO3C020328.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity