MELO3C018570.jh1 (gene) Melon (Harukei-3) v1.41

BLAST of MELO3C018570.jh1 vs. NCBI nr
Match: XP_008455102.1 (PREDICTED: K(+) efflux antiporter 3, chloroplastic [Cucumis melo])

HSP 1 Score: 1530 bits (3962), Expect = 0.0
Identity = 811/811 (100.00%), Postives = 811/811 (100.00%), Query Frame = 0

Query: 1   MSFGSMLEPVSCCQSSQSQIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC 60
           MSFGSMLEPVSCCQSSQSQIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC
Sbjct: 1   MSFGSMLEPVSCCQSSQSQIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC 60

Query: 61  TFNHWRNDYSLVPVLFHNGATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDV 120
           TFNHWRNDYSLVPVLFHNGATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDV
Sbjct: 61  TFNHWRNDYSLVPVLFHNGATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDV 120

Query: 121 INDLGLDTLTFLAVTVVVVPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGI 180
           INDLGLDTLTFLAVTVVVVPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGI
Sbjct: 121 INDLGLDTLTFLAVTVVVVPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGI 180

Query: 181 LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHA 240
           LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHA
Sbjct: 181 LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHA 240

Query: 241 RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPL 300
           RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPL
Sbjct: 241 RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPL 300

Query: 301 LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV 360
           LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV
Sbjct: 301 LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV 360

Query: 361 ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT 420
           ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT
Sbjct: 361 ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT 420

Query: 421 GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF 480
           GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF
Sbjct: 421 GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF 480

Query: 481 VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN 540
           VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN
Sbjct: 481 VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN 540

Query: 541 FDATEPVVIVGFGQMGQVLANFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPV 600
           FDATEPVVIVGFGQMGQVLANFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPV
Sbjct: 541 FDATEPVVIVGFGQMGQVLANFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPV 600

Query: 601 LYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLD 660
           LYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLD
Sbjct: 601 LYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLD 660

Query: 661 LKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQ 720
           LKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQ
Sbjct: 661 LKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQ 720

Query: 721 ELEIMKPLQIRVKDSIESPESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGV 780
           ELEIMKPLQIRVKDSIESPESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGV
Sbjct: 721 ELEIMKPLQIRVKDSIESPESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGV 780

Query: 781 LYCELDTENNLFEQNVVEPPTHITATDGLEK 811
           LYCELDTENNLFEQNVVEPPTHITATDGLEK
Sbjct: 781 LYCELDTENNLFEQNVVEPPTHITATDGLEK 811

BLAST of MELO3C018570.jh1 vs. NCBI nr
Match: XP_011658846.1 (K(+) efflux antiporter 3, chloroplastic [Cucumis sativus] >KGN43796.1 hypothetical protein Csa_017320 [Cucumis sativus])

HSP 1 Score: 1493 bits (3865), Expect = 0.0
Identity = 790/812 (97.29%), Postives = 803/812 (98.89%), Query Frame = 0

Query: 1   MSFGSMLEPVSCCQSSQSQIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC 60
           MSFG+MLEPVSCCQSSQSQIY AVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC
Sbjct: 1   MSFGTMLEPVSCCQSSQSQIYGAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC 60

Query: 61  TFNHWRNDYSLVPVLFHNGATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDV 120
           T N+WRNDYSLVPVLFHNGATTLTFKVVGQNGYNWSNRRPKQRER+RT AALDVAAAVDV
Sbjct: 61  TSNYWRNDYSLVPVLFHNGATTLTFKVVGQNGYNWSNRRPKQRERIRTHAALDVAAAVDV 120

Query: 121 INDLGLDTLTFLAVTVVVVPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGI 180
           INDLGLDTLTFLAVTVVVVPLFR++KASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGI
Sbjct: 121 INDLGLDTLTFLAVTVVVVPLFRRIKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGI 180

Query: 181 LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHA 240
           LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHA
Sbjct: 181 LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHA 240

Query: 241 RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPL 300
           RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPL
Sbjct: 241 RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPL 300

Query: 301 LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV 360
           LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV
Sbjct: 301 LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV 360

Query: 361 ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT 420
           ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT
Sbjct: 361 ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT 420

Query: 421 GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF 480
           GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF
Sbjct: 421 GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF 480

Query: 481 VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN 540
           VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN
Sbjct: 481 VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN 540

Query: 541 FDATEPVVIVGFGQMGQVLANFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPV 600
           FDATEPVVIVGFGQMGQVLANFLSTPLASG+DGNTPGWPYVAFDIDLSVVKTSRKLGFPV
Sbjct: 541 FDATEPVVIVGFGQMGQVLANFLSTPLASGLDGNTPGWPYVAFDIDLSVVKTSRKLGFPV 600

Query: 601 LYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLD 660
           LYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLD
Sbjct: 601 LYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLD 660

Query: 661 LKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQ 720
           LKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDA+DKSNEQ
Sbjct: 661 LKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDAIDKSNEQ 720

Query: 721 ELEIMKPLQIRVKDSIESPESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGV 780
           ELEIMKPLQIRVKDSIESPE+ELSRLN +DKTQILNGKEVDQMKQGTVF+K EDLDGNGV
Sbjct: 721 ELEIMKPLQIRVKDSIESPENELSRLNLKDKTQILNGKEVDQMKQGTVFEKAEDLDGNGV 780

Query: 781 LYCELDTENNLFEQNVVEPPT-HITATDGLEK 811
           LYC+LDTENN  E+NVVEPPT HI ATDG+EK
Sbjct: 781 LYCDLDTENNFLEENVVEPPTTHIAATDGVEK 812

BLAST of MELO3C018570.jh1 vs. NCBI nr
Match: ADN34254.1 (glutathione-regulated potassium-efflux system protein kefb [Cucumis melo subsp. melo])

HSP 1 Score: 1477 bits (3823), Expect = 0.0
Identity = 787/793 (99.24%), Postives = 787/793 (99.24%), Query Frame = 0

Query: 19  QIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSCTFNHWRNDYSLVPVLFHN 78
           QIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSCTFNHWRNDYSLVPVLFHN
Sbjct: 2   QIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSCTFNHWRNDYSLVPVLFHN 61

Query: 79  GATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDVINDLGLDTLTFLAVTVVV 138
           GATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDVINDLGLDTLTFLAVTVVV
Sbjct: 62  GATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDVINDLGLDTLTFLAVTVVV 121

Query: 139 VPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGILFLLFEMGLELSFARLKA 198
           VPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGILFLLFEMGLELSFARLKA
Sbjct: 122 VPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGILFLLFEMGLELSFARLKA 181

Query: 199 LARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARSDLVNIRSVDEAIVIGA 258
           LARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARSDLVNIRSVDEAIVIGA
Sbjct: 182 LARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARSDLVNIRSVDEAIVIGA 241

Query: 259 ALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPLLVILPVLESQNLGTESIW 318
           ALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPLLVILPVLESQNLGTESIW
Sbjct: 242 ALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPLLVILPVLESQNLGTESIW 301

Query: 319 PMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFVALCLLTVAGTSLITQKLG 378
           PMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFVALCLLTVAGTSLITQKLG
Sbjct: 302 PMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFVALCLLTVAGTSLITQKLG 361

Query: 379 FSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDMQLLFREWPNVLA 438
           FSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDMQLLFREWPNVLA
Sbjct: 362 FSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDMQLLFREWPNVLA 421

Query: 439 LLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGFVVFSLANRLGVLPLELNK 498
           LLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGFVV      LGVLPLELNK
Sbjct: 422 LLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGFVV------LGVLPLELNK 481

Query: 499 LLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVNFDATEPVVIVGFGQMGQV 558
           LLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVNFDATEPVVIVGFGQMGQV
Sbjct: 482 LLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVNFDATEPVVIVGFGQMGQV 541

Query: 559 LANFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPVLYGDGSRPAVLQSAGISS 618
           LANFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPVLYGDGSRPAVLQSAGISS
Sbjct: 542 LANFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPVLYGDGSRPAVLQSAGISS 601

Query: 619 PKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLDLKTAGATDAILEDAETSL 678
           PKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLDLKTAGATDAILEDAETSL
Sbjct: 602 PKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLDLKTAGATDAILEDAETSL 661

Query: 679 QLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQELEIMKPLQIRVKDSIES 738
           QLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQELEIMKPLQIRVKDSIES
Sbjct: 662 QLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQELEIMKPLQIRVKDSIES 721

Query: 739 PESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGVLYCELDTENNLFEQNVVE 798
           PESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGVLYCELDTENNLFEQNVVE
Sbjct: 722 PESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGVLYCELDTENNLFEQNVVE 781

Query: 799 PPTHITATDGLEK 811
           PPTHITATDGLEK
Sbjct: 782 PPTHITATDGLEK 788

BLAST of MELO3C018570.jh1 vs. NCBI nr
Match: KAA0031415.1 (K(+) efflux antiporter 3 [Cucumis melo var. makuwa] >TYK06866.1 K(+) efflux antiporter 3 [Cucumis melo var. makuwa])

HSP 1 Score: 1467 bits (3798), Expect = 0.0
Identity = 783/793 (98.74%), Postives = 783/793 (98.74%), Query Frame = 0

Query: 19  QIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSCTFNHWRNDYSLVPVLFHN 78
           QIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSCTFNHWRNDYSLVPVLFHN
Sbjct: 2   QIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSCTFNHWRNDYSLVPVLFHN 61

Query: 79  GATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDVINDLGLDTLTFLAVTVVV 138
           GATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDVINDLGLDTLTFLAVTVVV
Sbjct: 62  GATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDVINDLGLDTLTFLAVTVVV 121

Query: 139 VPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGILFLLFEMGLELSFARLKA 198
           VPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGILFLLFEMGLELSFARLKA
Sbjct: 122 VPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGILFLLFEMGLELSFARLKA 181

Query: 199 LARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARSDLVNIRSVDEAIVIGA 258
           LARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARSDLVNIRSVDEAIVIGA
Sbjct: 182 LARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARSDLVNIRSVDEAIVIGA 241

Query: 259 ALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPLLVILPVLESQNLGTESIW 318
           ALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPLLVILPVLESQNLGTESIW
Sbjct: 242 ALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPLLVILPVLESQNLGTESIW 301

Query: 319 PMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFVALCLLTVAGTSLITQKLG 378
           PMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFVALCLLTVAGTSLITQKLG
Sbjct: 302 PMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFVALCLLTVAGTSLITQKLG 361

Query: 379 FSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDMQLLFREWPNVLA 438
           FSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDMQLLFREWPNVLA
Sbjct: 362 FSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDMQLLFREWPNVLA 421

Query: 439 LLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGFVVFSLANRLGVLPLELNK 498
           LLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGFVVFSLANRLGVLPLELNK
Sbjct: 422 LLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGFVVFSLANRLGVLPLELNK 481

Query: 499 LLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVNFDATEPVVIVGFGQMGQV 558
           LLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVNFDATEPVV          
Sbjct: 482 LLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVNFDATEPVV---------- 541

Query: 559 LANFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPVLYGDGSRPAVLQSAGISS 618
           LANFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPVLYGDGSRPAVLQSAGISS
Sbjct: 542 LANFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPVLYGDGSRPAVLQSAGISS 601

Query: 619 PKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLDLKTAGATDAILEDAETSL 678
           PKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLDLKTAGATDAILEDAETSL
Sbjct: 602 PKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLDLKTAGATDAILEDAETSL 661

Query: 679 QLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQELEIMKPLQIRVKDSIES 738
           QLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQELEIMKPLQIRVKDSIES
Sbjct: 662 QLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQELEIMKPLQIRVKDSIES 721

Query: 739 PESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGVLYCELDTENNLFEQNVVE 798
           PESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGVLYCELDTENNLFEQNVVE
Sbjct: 722 PESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGVLYCELDTENNLFEQNVVE 781

Query: 799 PPTHITATDGLEK 811
           PPTHITATDGLEK
Sbjct: 782 PPTHITATDGLEK 784

BLAST of MELO3C018570.jh1 vs. NCBI nr
Match: XP_038887837.1 (K(+) efflux antiporter 3, chloroplastic [Benincasa hispida])

HSP 1 Score: 1465 bits (3792), Expect = 0.0
Identity = 778/811 (95.93%), Postives = 793/811 (97.78%), Query Frame = 0

Query: 1   MSFGSMLEPVSCCQSSQSQIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC 60
           MS G+MLE VSCCQSSQSQIYSAVKQKNP RAYSHTVHQLCGPSFNLHYSHSKK+AV SC
Sbjct: 1   MSLGNMLESVSCCQSSQSQIYSAVKQKNPLRAYSHTVHQLCGPSFNLHYSHSKKLAVSSC 60

Query: 61  TFNHWRNDYSLVPVLFHNGATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDV 120
           T N  RNDYSLVPVLF++GATTLTFKVV QNGY+WSNRRPKQR RL TRAALDV+AAVDV
Sbjct: 61  TLNCGRNDYSLVPVLFYSGATTLTFKVVRQNGYSWSNRRPKQRIRLGTRAALDVSAAVDV 120

Query: 121 INDLGLDTLTFLAVTVVVVPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGI 180
           INDLGLDTLTFLAVTVVVVPLFR++KASPILGFFFAGIVLNQFGVIRNI+DVKVLSEWGI
Sbjct: 121 INDLGLDTLTFLAVTVVVVPLFRRIKASPILGFFFAGIVLNQFGVIRNIIDVKVLSEWGI 180

Query: 181 LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHA 240
           LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGA+GT+ILE+LFHA
Sbjct: 181 LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAIGTRILEYLFHA 240

Query: 241 RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPL 300
           RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPL
Sbjct: 241 RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPL 300

Query: 301 LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV 360
           LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV
Sbjct: 301 LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV 360

Query: 361 ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT 420
           ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT
Sbjct: 361 ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT 420

Query: 421 GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF 480
           GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF
Sbjct: 421 GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF 480

Query: 481 VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN 540
           VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN
Sbjct: 481 VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN 540

Query: 541 FDATEPVVIVGFGQMGQVLANFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPV 600
           FDATEPVVIVGFGQMGQVLANFLSTPLASG DGNTPGWPYVAFDIDLSVVKTSRKLGFPV
Sbjct: 541 FDATEPVVIVGFGQMGQVLANFLSTPLASGTDGNTPGWPYVAFDIDLSVVKTSRKLGFPV 600

Query: 601 LYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLD 660
           LYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLD
Sbjct: 601 LYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLD 660

Query: 661 LKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQ 720
           LK AGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQ
Sbjct: 661 LKNAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQ 720

Query: 721 ELEIMKPLQIRVKDSIESPESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGV 780
           ELE+MKPLQIRVKDSIESPE+ELSRLNRED TQILNGKEVDQMK  T FQKPEDLDGNGV
Sbjct: 721 ELELMKPLQIRVKDSIESPENELSRLNREDNTQILNGKEVDQMKPDTFFQKPEDLDGNGV 780

Query: 781 LYCELDTENNLFEQNVVEPPTHITATDGLEK 811
           LYCEL+TENN  EQNVVEPPTHITATDGLEK
Sbjct: 781 LYCELETENNFLEQNVVEPPTHITATDGLEK 811

BLAST of MELO3C018570.jh1 vs. ExPASy Swiss-Prot
Match: Q9M0Z3 (K(+) efflux antiporter 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=KEA3 PE=1 SV=2)

HSP 1 Score: 939.5 bits (2427), Expect = 2.5e-272
Identity = 523/765 (68.37%), Postives = 604/765 (78.95%), Query Frame = 0

Query: 1   MSFGSMLEPVSCCQSSQSQIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC 60
           M+  +ML  +SCC S +   Y  VKQ        H+V          H   + K +VP  
Sbjct: 1   MAISTMLGSISCCPSPKG--YEMVKQ--------HSVRL-------KHCVFTVKSSVP-- 60

Query: 61  TFNHWRNDYSLVPVLFHNGATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDV 120
            ++   ND  +    F N      F    +  Y           R+ T A +DVA+AVDV
Sbjct: 61  VYSEGVND-GIKLHSFGNLVKKKVFLDTSKRFYFQGRWSESSGRRVETYAGVDVASAVDV 120

Query: 121 INDLGLDTLTFLAVTVVVVPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGI 180
           INDLG DTLTFL VTV++VP FR +KASPILGFFFAG+VLNQFG+IRN+ DVKVLSEWGI
Sbjct: 121 INDLGFDTLTFLMVTVIIVPAFRILKASPILGFFFAGVVLNQFGLIRNLTDVKVLSEWGI 180

Query: 181 LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHA 240
           LFLLFEMGLELS ARLKALA+FAFGMGLTQV+L T AFTAFELP NGA+GTKILEFLFH+
Sbjct: 181 LFLLFEMGLELSLARLKALAKFAFGMGLTQVLLCTAAFTAFELPPNGAIGTKILEFLFHS 240

Query: 241 RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPL 300
           R DLVNIRS+DEA+VIGAALSLSSSAFVLQLLAEKGEL TRFGSATLGILLLQDIAVVPL
Sbjct: 241 RPDLVNIRSIDEAVVIGAALSLSSSAFVLQLLAEKGELPTRFGSATLGILLLQDIAVVPL 300

Query: 301 LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV 360
           LVILPVLESQ++G ESIWPMLA+ES KALGGLG+LSLGGK  LRR+FEVVAE RSSEAFV
Sbjct: 301 LVILPVLESQDIGGESIWPMLAKESAKALGGLGILSLGGKFFLRRIFEVVAETRSSEAFV 360

Query: 361 ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT 420
           ALCLLTVAGTSL+TQ LGFSDTLGAFLAGA+LAETNFRTQIEADIRPFRGLLLGLFFVTT
Sbjct: 361 ALCLLTVAGTSLVTQWLGFSDTLGAFLAGALLAETNFRTQIEADIRPFRGLLLGLFFVTT 420

Query: 421 GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF 480
           GTSIDM++LFREWPNVL+LL GLI IKTLIITAIGPRVGLT QESVR+GFLLSQGGEF F
Sbjct: 421 GTSIDMEVLFREWPNVLSLLGGLIVIKTLIITAIGPRVGLTIQESVRVGFLLSQGGEFAF 480

Query: 481 VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN 540
           VVFSLANRLGVLP ELNKLLII+VVLSMALTP LN+ GRKA++F+ E+    +K  + VN
Sbjct: 481 VVFSLANRLGVLPNELNKLLIIVVVLSMALTPYLNQLGRKAADFLDERLDPGEKIGEDVN 540

Query: 541 FDATEPVVIVGFGQMGQVLANFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPV 600
           FD +E +VI+GFGQMGQVLANFLSTPL S  D +  GWPY+ FD++ +VVK SRKLGFP+
Sbjct: 541 FDVSESIVIIGFGQMGQVLANFLSTPLVS--DSDLVGWPYIGFDLNPAVVKESRKLGFPI 600

Query: 601 LYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLD 660
           LYGDGSRP+VLQSAG+SSPKA+M+M+  KK T +AVQ+LRLAFP  PIYARA+D+ HLL+
Sbjct: 601 LYGDGSRPSVLQSAGVSSPKAIMIMYKGKKRTTEAVQRLRLAFPGSPIYARAQDLPHLLE 660

Query: 661 LKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKS--- 720
           LK AGATDAILE+AETSLQLGSKLL G GVMSD VSFLS++ R+SMEIQAQ+ +  S   
Sbjct: 661 LKKAGATDAILENAETSLQLGSKLLTGFGVMSDDVSFLSKVFRDSMEIQAQEEITASETN 720

Query: 721 -------------------NEQELEIMKPLQIRVKDSIESPESEL 744
                               ++++++MKP+Q++  DS     +E+
Sbjct: 721 AGLKPMQMKASDINVVSAATQKQVQLMKPMQMKASDSNSDSAAEI 743

BLAST of MELO3C018570.jh1 vs. ExPASy Swiss-Prot
Match: O65272 (K(+) efflux antiporter 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=KEA2 PE=1 SV=2)

HSP 1 Score: 339.3 bits (869), Expect = 1.2e-91
Identity = 216/571 (37.83%), Postives = 323/571 (56.57%), Query Frame = 0

Query: 126  LDTLTFLAVTVVVVPLFRKVK-ASPILGFFFAGIVLNQFG--VIRNIVDVKVLSEWGILF 185
            LD L  L  +V+ VPLF+K+   SP+LG+  AGI++  +G  +IRN+   K ++E+G++F
Sbjct: 570  LDVLWLLLASVIFVPLFQKIPGGSPVLGYLAAGILIGPYGLSIIRNVHGTKAIAEFGVVF 629

Query: 186  LLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARS 245
            LLF +GLELS  RL ++ ++ FG+G  QV+++            G  G            
Sbjct: 630  LLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAAVIGLITHYVAGQAG------------ 689

Query: 246  DLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPLLV 305
                      AIVIG  L+LSS+A VLQ+L E+GE  +R G AT  +LL QD+AVV LL+
Sbjct: 690  --------PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLI 749

Query: 306  ILPVLESQN----LGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEA 365
            ++P++   +    +G ++I   L   ++KA   +  +  GG+L+LR +++ +AE R++E 
Sbjct: 750  LIPLISPNSSKGGIGFQAIAEALGLAAIKAAVAITGIIAGGRLLLRPIYKQIAENRNAEI 809

Query: 366  FVALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFV 425
            F A  LL + GTSL+T + G S  LGAFLAG +LAET F  Q+E+DI P+RGLLLGLFF+
Sbjct: 810  FSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFM 869

Query: 426  TTGTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEF 485
            T G SID +LL   +P ++  L  L+  KT+++  IG   G++   +VR+G LL+ GGEF
Sbjct: 870  TVGMSIDPKLLLANFPLIMGTLGLLLVGKTILVVIIGKLFGISIISAVRVGLLLAPGGEF 929

Query: 486  GFVVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAA-- 545
             FV F  A   G++  +L+ LL ++V +SMALTP L   G    + I+ +++ +D  +  
Sbjct: 930  AFVAFGEAVNQGIMTPQLSSLLFLVVGISMALTPWLAAGG----QLIASRFELQDVRSLL 989

Query: 546  --DTVNFDATEPVVIVGFGQMGQVLANFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSR 605
              ++   D    ++I GFG++GQ++A  LS  L           P+VA D+    V   R
Sbjct: 990  PVESETDDLQGHIIICGFGRIGQIIAQLLSERLI----------PFVALDVSSDRVAIGR 1049

Query: 606  KLGFPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKD 665
             L  PV +GD     VL   G     A  +      A    V  L   FP +  + RA D
Sbjct: 1050 SLDLPVYFGDAGSREVLHKIGADRACAAAIALDTPGANYRCVWALSKYFPNVKTFVRAHD 1106

Query: 666  VVHLLDLKTAGATDAILEDAETSLQLGSKLL 686
            V H L+L+ AGAT  + E  E SLQL + +L
Sbjct: 1110 VDHGLNLEKAGATAVVPETLEPSLQLAAAVL 1106

BLAST of MELO3C018570.jh1 vs. ExPASy Swiss-Prot
Match: Q9ZTZ7 (K(+) efflux antiporter 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=KEA1 PE=1 SV=2)

HSP 1 Score: 335.5 bits (859), Expect = 1.7e-90
Identity = 212/570 (37.19%), Postives = 323/570 (56.67%), Query Frame = 0

Query: 127  DTLTFLAVTVVVVPLFRKVK-ASPILGFFFAGIVLNQFG--VIRNIVDVKVLSEWGILFL 186
            D L  L  +V+ VPLF+K+   SP+LG+  AGI++  +G  +IRN+   + ++E+G++FL
Sbjct: 591  DFLWLLLASVIFVPLFQKIPGGSPVLGYLAAGILIGPYGLSIIRNVHGTRAIAEFGVVFL 650

Query: 187  LFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARSD 246
            LF +GLELS  RL ++ ++ FG+G  QV+++            G  G             
Sbjct: 651  LFNIGLELSVERLSSMKKYVFGLGSAQVLVTAAVVGLLAHYVAGQAG------------- 710

Query: 247  LVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPLLVI 306
                     AIVIG  L+LSS+A VLQ+L E+GE  +R G A+  +LL QD+AVV LL++
Sbjct: 711  -------PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRASFSVLLFQDLAVVVLLIL 770

Query: 307  LPVLESQN----LGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAF 366
            +P++   +    +G ++I   L   ++KA   +  +  GG+L+LR +++ +AE R++E F
Sbjct: 771  IPLISPNSSKGGIGFQAIAEALGLAAVKAAVAITAIIAGGRLLLRPIYKQIAENRNAEIF 830

Query: 367  VALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVT 426
             A  LL + GTSL+T + G S  LGAFLAG +LAET F  Q+E+DI P+RGLLLGLFF+T
Sbjct: 831  SANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMT 890

Query: 427  TGTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFG 486
             G SID +LL   +P ++  L  LI  KT+++  +G   G++   ++R+G LL+ GGEF 
Sbjct: 891  VGMSIDPKLLLSNFPVIVGTLGLLIVGKTMLVVIMGKLFGISIISAIRVGLLLAPGGEFA 950

Query: 487  FVVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAA--- 546
            FV F  A   G++  +L+ LL ++V +SMA+TP L   G    + I+ +++  D  +   
Sbjct: 951  FVAFGEAVNQGIMSPQLSSLLFLVVGISMAITPWLAAGG----QLIASRFELHDVRSLLP 1010

Query: 547  -DTVNFDATEPVVIVGFGQMGQVLANFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSRK 606
             ++   D    ++I GFG++GQ++A  LS  L           P+VA D+    V   R 
Sbjct: 1011 VESETDDLQGHIIICGFGRVGQIIAQLLSERLI----------PFVALDVSSDRVTIGRS 1070

Query: 607  LGFPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDV 666
            L  PV +GD     VL   G     A +V      A    V  L   +P +  + RA DV
Sbjct: 1071 LDLPVYFGDAGSKEVLHKIGAGRACAAVVALDAPGANYRCVWALSKFYPNVKTFVRAHDV 1126

Query: 667  VHLLDLKTAGATDAILEDAETSLQLGSKLL 686
            VH L+L+ AGAT  + E  E SLQL + +L
Sbjct: 1131 VHGLNLEKAGATAVVPETLEPSLQLAAAVL 1126

BLAST of MELO3C018570.jh1 vs. ExPASy Swiss-Prot
Match: Q1C2S9 (Glutathione-regulated potassium-efflux system protein KefB OS=Yersinia pestis bv. Antiqua (strain Antiqua) OX=360102 GN=kefB PE=3 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 2.1e-69
Identity = 194/578 (33.56%), Postives = 305/578 (52.77%), Query Frame = 0

Query: 129 LTFLAVTVVVVPLFRKVKASPILGFFFAGIVLNQFGV--IRNIVDVKVLSEWGILFLLFE 188
           L FL   VV VP+ +++    +LG+  AGI +  +G+  IR++ ++   SE G++FL+F 
Sbjct: 11  LVFLFAAVVAVPIAQRLGIGAVLGYLIAGIAIGPWGLGFIRDVDEILHFSELGVVFLMFI 70

Query: 189 MGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARSDLVN 248
           +GLEL+ A+L  L R  FG+G  QV+++     A    T         +F + A      
Sbjct: 71  IGLELNPAKLWQLRRSIFGVGAGQVVITAAVLGALLYFT---------QFAWQA------ 130

Query: 249 IRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPLLVILPV 308
                 A++ G  L++SS+A  LQL+ EKG      G     +LL QD+AV+P L ++P+
Sbjct: 131 ------AVIGGVGLAMSSTAMALQLMREKGMNRNEGGQLGFSVLLFQDMAVIPALALIPI 190

Query: 309 LESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFVALCLLT 368
           L + N G  + W  +    LK     G+L +GG+ +LR +F  +  +   E F A  LL 
Sbjct: 191 L-AGNEGGANDWVKI---GLKIAAFAGML-IGGRYLLRPLFRYIVASGVREVFTAAALLV 250

Query: 369 VAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDM 428
           V G++L    LG S  LG F+AG +LAE+ F+ ++E  I PF+GLLLGLFF++ G ++D+
Sbjct: 251 VLGSALFMDALGLSMALGTFIAGILLAESEFQHELEIAIEPFKGLLLGLFFISVGMALDL 310

Query: 429 QLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGFVVFSLA 488
            +LF    +VL  +  L+ IK+ I+  +    GL     ++   +LSQGGEF FV+FS A
Sbjct: 311 GVLFTHLLDVLLGVLALVFIKSAILYGLARVFGLRRSVRLQFAGVLSQGGEFAFVLFSAA 370

Query: 489 NRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVNFDATEP 548
               VL  E   LL+++V LSM  TPLL +        +  +Y  ++++ +    +  +P
Sbjct: 371 FSQRVLNAEQLALLLVVVTLSMMTTPLLMQV---IDRILVRRYNAQEESDEKPFVEDNDP 430

Query: 549 -VVIVGFGQMGQVLANFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPVLYGDG 608
            V+IVGFG+ GQV+   L   +A+ +            + D+S V   RK G+ V YGD 
Sbjct: 431 QVIIVGFGRFGQVIGRLL---MANKM-------RITVLERDVSAVSMMRKYGYKVYYGDA 490

Query: 609 SRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLDLKTAG 668
           +   +L++AG    KA+++   E + T+  V   +  FP + I ARA+  V   +L   G
Sbjct: 491 TELELLRAAGAEKAKAIVITCNEPEDTMALVHLCQQHFPNLHILARARGRVEAHELLQNG 549

Query: 669 ATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVR 704
             D   E   ++L+LG K L  LG+   Q     Q  R
Sbjct: 551 VKDFTRETFSSALELGRKTLLELGMHPHQAYRAQQHFR 549

BLAST of MELO3C018570.jh1 vs. ExPASy Swiss-Prot
Match: Q8ZJC4 (Glutathione-regulated potassium-efflux system protein KefB OS=Yersinia pestis OX=632 GN=kefB PE=3 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 2.1e-69
Identity = 194/578 (33.56%), Postives = 305/578 (52.77%), Query Frame = 0

Query: 129 LTFLAVTVVVVPLFRKVKASPILGFFFAGIVLNQFGV--IRNIVDVKVLSEWGILFLLFE 188
           L FL   VV VP+ +++    +LG+  AGI +  +G+  IR++ ++   SE G++FL+F 
Sbjct: 11  LVFLFAAVVAVPIAQRLGIGAVLGYLIAGIAIGPWGLGFIRDVDEILHFSELGVVFLMFI 70

Query: 189 MGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARSDLVN 248
           +GLEL+ A+L  L R  FG+G  QV+++     A    T         +F + A      
Sbjct: 71  IGLELNPAKLWQLRRSIFGVGAGQVVITAAVLGALLYFT---------QFAWQA------ 130

Query: 249 IRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPLLVILPV 308
                 A++ G  L++SS+A  LQL+ EKG      G     +LL QD+AV+P L ++P+
Sbjct: 131 ------AVIGGVGLAMSSTAMALQLMREKGMNRNEGGQLGFSVLLFQDMAVIPALALIPI 190

Query: 309 LESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFVALCLLT 368
           L + N G  + W  +    LK     G+L +GG+ +LR +F  +  +   E F A  LL 
Sbjct: 191 L-AGNEGGANDWVKI---GLKIAAFAGML-IGGRYLLRPLFRYIVASGVREVFTAAALLV 250

Query: 369 VAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDM 428
           V G++L    LG S  LG F+AG +LAE+ F+ ++E  I PF+GLLLGLFF++ G ++D+
Sbjct: 251 VLGSALFMDALGLSMALGTFIAGILLAESEFQHELEIAIEPFKGLLLGLFFISVGMALDL 310

Query: 429 QLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGFVVFSLA 488
            +LF    +VL  +  L+ IK+ I+  +    GL     ++   +LSQGGEF FV+FS A
Sbjct: 311 GVLFTHLLDVLLGVLALVFIKSAILYGLARVFGLRRSVRLQFAGVLSQGGEFAFVLFSAA 370

Query: 489 NRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVNFDATEP 548
               VL  E   LL+++V LSM  TPLL +        +  +Y  ++++ +    +  +P
Sbjct: 371 FSQRVLNAEQLALLLVVVTLSMMTTPLLMQV---IDRILVRRYNAQEESDEKPFVEDNDP 430

Query: 549 -VVIVGFGQMGQVLANFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPVLYGDG 608
            V+IVGFG+ GQV+   L   +A+ +            + D+S V   RK G+ V YGD 
Sbjct: 431 QVIIVGFGRFGQVIGRLL---MANKM-------RITVLERDVSAVSMMRKYGYKVYYGDA 490

Query: 609 SRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLDLKTAG 668
           +   +L++AG    KA+++   E + T+  V   +  FP + I ARA+  V   +L   G
Sbjct: 491 TELELLRAAGAEKAKAIVITCNEPEDTMALVHLCQQHFPNLHILARARGRVEAHELLQNG 549

Query: 669 ATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVR 704
             D   E   ++L+LG K L  LG+   Q     Q  R
Sbjct: 551 VKDFTRETFSSALELGRKTLLELGMHPHQAYRAQQHFR 549

BLAST of MELO3C018570.jh1 vs. ExPASy TrEMBL
Match: A0A1S3C1C7 (K(+) efflux antiporter 3, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103495357 PE=4 SV=1)

HSP 1 Score: 1530 bits (3962), Expect = 0.0
Identity = 811/811 (100.00%), Postives = 811/811 (100.00%), Query Frame = 0

Query: 1   MSFGSMLEPVSCCQSSQSQIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC 60
           MSFGSMLEPVSCCQSSQSQIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC
Sbjct: 1   MSFGSMLEPVSCCQSSQSQIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC 60

Query: 61  TFNHWRNDYSLVPVLFHNGATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDV 120
           TFNHWRNDYSLVPVLFHNGATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDV
Sbjct: 61  TFNHWRNDYSLVPVLFHNGATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDV 120

Query: 121 INDLGLDTLTFLAVTVVVVPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGI 180
           INDLGLDTLTFLAVTVVVVPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGI
Sbjct: 121 INDLGLDTLTFLAVTVVVVPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGI 180

Query: 181 LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHA 240
           LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHA
Sbjct: 181 LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHA 240

Query: 241 RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPL 300
           RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPL
Sbjct: 241 RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPL 300

Query: 301 LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV 360
           LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV
Sbjct: 301 LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV 360

Query: 361 ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT 420
           ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT
Sbjct: 361 ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT 420

Query: 421 GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF 480
           GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF
Sbjct: 421 GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF 480

Query: 481 VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN 540
           VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN
Sbjct: 481 VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN 540

Query: 541 FDATEPVVIVGFGQMGQVLANFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPV 600
           FDATEPVVIVGFGQMGQVLANFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPV
Sbjct: 541 FDATEPVVIVGFGQMGQVLANFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPV 600

Query: 601 LYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLD 660
           LYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLD
Sbjct: 601 LYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLD 660

Query: 661 LKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQ 720
           LKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQ
Sbjct: 661 LKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQ 720

Query: 721 ELEIMKPLQIRVKDSIESPESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGV 780
           ELEIMKPLQIRVKDSIESPESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGV
Sbjct: 721 ELEIMKPLQIRVKDSIESPESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGV 780

Query: 781 LYCELDTENNLFEQNVVEPPTHITATDGLEK 811
           LYCELDTENNLFEQNVVEPPTHITATDGLEK
Sbjct: 781 LYCELDTENNLFEQNVVEPPTHITATDGLEK 811

BLAST of MELO3C018570.jh1 vs. ExPASy TrEMBL
Match: A0A0A0K7K4 (RCK N-terminal domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G067510 PE=4 SV=1)

HSP 1 Score: 1493 bits (3865), Expect = 0.0
Identity = 790/812 (97.29%), Postives = 803/812 (98.89%), Query Frame = 0

Query: 1   MSFGSMLEPVSCCQSSQSQIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC 60
           MSFG+MLEPVSCCQSSQSQIY AVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC
Sbjct: 1   MSFGTMLEPVSCCQSSQSQIYGAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC 60

Query: 61  TFNHWRNDYSLVPVLFHNGATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDV 120
           T N+WRNDYSLVPVLFHNGATTLTFKVVGQNGYNWSNRRPKQRER+RT AALDVAAAVDV
Sbjct: 61  TSNYWRNDYSLVPVLFHNGATTLTFKVVGQNGYNWSNRRPKQRERIRTHAALDVAAAVDV 120

Query: 121 INDLGLDTLTFLAVTVVVVPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGI 180
           INDLGLDTLTFLAVTVVVVPLFR++KASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGI
Sbjct: 121 INDLGLDTLTFLAVTVVVVPLFRRIKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGI 180

Query: 181 LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHA 240
           LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHA
Sbjct: 181 LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHA 240

Query: 241 RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPL 300
           RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPL
Sbjct: 241 RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPL 300

Query: 301 LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV 360
           LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV
Sbjct: 301 LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV 360

Query: 361 ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT 420
           ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT
Sbjct: 361 ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT 420

Query: 421 GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF 480
           GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF
Sbjct: 421 GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF 480

Query: 481 VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN 540
           VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN
Sbjct: 481 VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN 540

Query: 541 FDATEPVVIVGFGQMGQVLANFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPV 600
           FDATEPVVIVGFGQMGQVLANFLSTPLASG+DGNTPGWPYVAFDIDLSVVKTSRKLGFPV
Sbjct: 541 FDATEPVVIVGFGQMGQVLANFLSTPLASGLDGNTPGWPYVAFDIDLSVVKTSRKLGFPV 600

Query: 601 LYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLD 660
           LYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLD
Sbjct: 601 LYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLD 660

Query: 661 LKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQ 720
           LKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDA+DKSNEQ
Sbjct: 661 LKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDAIDKSNEQ 720

Query: 721 ELEIMKPLQIRVKDSIESPESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGV 780
           ELEIMKPLQIRVKDSIESPE+ELSRLN +DKTQILNGKEVDQMKQGTVF+K EDLDGNGV
Sbjct: 721 ELEIMKPLQIRVKDSIESPENELSRLNLKDKTQILNGKEVDQMKQGTVFEKAEDLDGNGV 780

Query: 781 LYCELDTENNLFEQNVVEPPT-HITATDGLEK 811
           LYC+LDTENN  E+NVVEPPT HI ATDG+EK
Sbjct: 781 LYCDLDTENNFLEENVVEPPTTHIAATDGVEK 812

BLAST of MELO3C018570.jh1 vs. ExPASy TrEMBL
Match: E5GCQ5 (Glutathione-regulated potassium-efflux system protein kefb OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 1477 bits (3823), Expect = 0.0
Identity = 787/793 (99.24%), Postives = 787/793 (99.24%), Query Frame = 0

Query: 19  QIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSCTFNHWRNDYSLVPVLFHN 78
           QIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSCTFNHWRNDYSLVPVLFHN
Sbjct: 2   QIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSCTFNHWRNDYSLVPVLFHN 61

Query: 79  GATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDVINDLGLDTLTFLAVTVVV 138
           GATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDVINDLGLDTLTFLAVTVVV
Sbjct: 62  GATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDVINDLGLDTLTFLAVTVVV 121

Query: 139 VPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGILFLLFEMGLELSFARLKA 198
           VPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGILFLLFEMGLELSFARLKA
Sbjct: 122 VPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGILFLLFEMGLELSFARLKA 181

Query: 199 LARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARSDLVNIRSVDEAIVIGA 258
           LARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARSDLVNIRSVDEAIVIGA
Sbjct: 182 LARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARSDLVNIRSVDEAIVIGA 241

Query: 259 ALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPLLVILPVLESQNLGTESIW 318
           ALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPLLVILPVLESQNLGTESIW
Sbjct: 242 ALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPLLVILPVLESQNLGTESIW 301

Query: 319 PMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFVALCLLTVAGTSLITQKLG 378
           PMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFVALCLLTVAGTSLITQKLG
Sbjct: 302 PMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFVALCLLTVAGTSLITQKLG 361

Query: 379 FSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDMQLLFREWPNVLA 438
           FSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDMQLLFREWPNVLA
Sbjct: 362 FSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDMQLLFREWPNVLA 421

Query: 439 LLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGFVVFSLANRLGVLPLELNK 498
           LLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGFVV      LGVLPLELNK
Sbjct: 422 LLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGFVV------LGVLPLELNK 481

Query: 499 LLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVNFDATEPVVIVGFGQMGQV 558
           LLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVNFDATEPVVIVGFGQMGQV
Sbjct: 482 LLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVNFDATEPVVIVGFGQMGQV 541

Query: 559 LANFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPVLYGDGSRPAVLQSAGISS 618
           LANFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPVLYGDGSRPAVLQSAGISS
Sbjct: 542 LANFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPVLYGDGSRPAVLQSAGISS 601

Query: 619 PKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLDLKTAGATDAILEDAETSL 678
           PKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLDLKTAGATDAILEDAETSL
Sbjct: 602 PKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLDLKTAGATDAILEDAETSL 661

Query: 679 QLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQELEIMKPLQIRVKDSIES 738
           QLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQELEIMKPLQIRVKDSIES
Sbjct: 662 QLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQELEIMKPLQIRVKDSIES 721

Query: 739 PESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGVLYCELDTENNLFEQNVVE 798
           PESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGVLYCELDTENNLFEQNVVE
Sbjct: 722 PESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGVLYCELDTENNLFEQNVVE 781

Query: 799 PPTHITATDGLEK 811
           PPTHITATDGLEK
Sbjct: 782 PPTHITATDGLEK 788

BLAST of MELO3C018570.jh1 vs. ExPASy TrEMBL
Match: A0A5A7SQK8 (K(+) efflux antiporter 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001700 PE=4 SV=1)

HSP 1 Score: 1467 bits (3798), Expect = 0.0
Identity = 783/793 (98.74%), Postives = 783/793 (98.74%), Query Frame = 0

Query: 19  QIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSCTFNHWRNDYSLVPVLFHN 78
           QIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSCTFNHWRNDYSLVPVLFHN
Sbjct: 2   QIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSCTFNHWRNDYSLVPVLFHN 61

Query: 79  GATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDVINDLGLDTLTFLAVTVVV 138
           GATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDVINDLGLDTLTFLAVTVVV
Sbjct: 62  GATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDVINDLGLDTLTFLAVTVVV 121

Query: 139 VPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGILFLLFEMGLELSFARLKA 198
           VPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGILFLLFEMGLELSFARLKA
Sbjct: 122 VPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGILFLLFEMGLELSFARLKA 181

Query: 199 LARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARSDLVNIRSVDEAIVIGA 258
           LARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARSDLVNIRSVDEAIVIGA
Sbjct: 182 LARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARSDLVNIRSVDEAIVIGA 241

Query: 259 ALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPLLVILPVLESQNLGTESIW 318
           ALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPLLVILPVLESQNLGTESIW
Sbjct: 242 ALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPLLVILPVLESQNLGTESIW 301

Query: 319 PMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFVALCLLTVAGTSLITQKLG 378
           PMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFVALCLLTVAGTSLITQKLG
Sbjct: 302 PMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFVALCLLTVAGTSLITQKLG 361

Query: 379 FSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDMQLLFREWPNVLA 438
           FSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDMQLLFREWPNVLA
Sbjct: 362 FSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDMQLLFREWPNVLA 421

Query: 439 LLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGFVVFSLANRLGVLPLELNK 498
           LLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGFVVFSLANRLGVLPLELNK
Sbjct: 422 LLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGFVVFSLANRLGVLPLELNK 481

Query: 499 LLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVNFDATEPVVIVGFGQMGQV 558
           LLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVNFDATEPVV          
Sbjct: 482 LLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVNFDATEPVV---------- 541

Query: 559 LANFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPVLYGDGSRPAVLQSAGISS 618
           LANFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPVLYGDGSRPAVLQSAGISS
Sbjct: 542 LANFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPVLYGDGSRPAVLQSAGISS 601

Query: 619 PKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLDLKTAGATDAILEDAETSL 678
           PKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLDLKTAGATDAILEDAETSL
Sbjct: 602 PKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLDLKTAGATDAILEDAETSL 661

Query: 679 QLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQELEIMKPLQIRVKDSIES 738
           QLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQELEIMKPLQIRVKDSIES
Sbjct: 662 QLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQELEIMKPLQIRVKDSIES 721

Query: 739 PESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGVLYCELDTENNLFEQNVVE 798
           PESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGVLYCELDTENNLFEQNVVE
Sbjct: 722 PESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGVLYCELDTENNLFEQNVVE 781

Query: 799 PPTHITATDGLEK 811
           PPTHITATDGLEK
Sbjct: 782 PPTHITATDGLEK 784

BLAST of MELO3C018570.jh1 vs. ExPASy TrEMBL
Match: A0A6J1E2E0 (K(+) efflux antiporter 3, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111025388 PE=4 SV=1)

HSP 1 Score: 1366 bits (3536), Expect = 0.0
Identity = 732/821 (89.16%), Postives = 768/821 (93.54%), Query Frame = 0

Query: 1   MSFGSMLEPVSCCQSSQSQIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC 60
           MS G+ML+PVSCCQSSQSQIYS VKQKNP RAYSH +HQLCGPSFNLH   SKKVAVPS 
Sbjct: 1   MSLGTMLDPVSCCQSSQSQIYSVVKQKNPMRAYSHIIHQLCGPSFNLHNLQSKKVAVPSF 60

Query: 61  TFNHWRNDYSLVPVLFHNGATTLTF---KVVGQNGYNWSNRRPKQRERLRTRAALDVAAA 120
           T N  RN YSLVPVLFHN +TTL F   KVVG++G+NWSN RPKQRERLRT AA+DVAAA
Sbjct: 61  TINCLRNGYSLVPVLFHNSSTTLAFSSSKVVGRDGFNWSNHRPKQRERLRTCAAVDVAAA 120

Query: 121 VDVINDLGLDTLTFLAVTVVVVPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSE 180
           VDVINDLGLDTLTFLAVTV+VVPLFR+VKASPILGFFFAGIVLNQFGVIRN++DVKVLSE
Sbjct: 121 VDVINDLGLDTLTFLAVTVIVVPLFRRVKASPILGFFFAGIVLNQFGVIRNLIDVKVLSE 180

Query: 181 WGILFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFL 240
           WGILFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELP NGA+GT+ILEFL
Sbjct: 181 WGILFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPPNGAIGTRILEFL 240

Query: 241 FHARSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAV 300
           FHARSDLVNIRS+DEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILL QDIAV
Sbjct: 241 FHARSDLVNIRSIDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILLFQDIAV 300

Query: 301 VPLLVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSE 360
           VPLLVILPVLESQNLGT+SIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSE
Sbjct: 301 VPLLVILPVLESQNLGTQSIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSE 360

Query: 361 AFVALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFF 420
           AFVALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFF
Sbjct: 361 AFVALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFF 420

Query: 421 VTTGTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGE 480
           VTTGTSID QLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIG LLSQGGE
Sbjct: 421 VTTGTSIDTQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGLLLSQGGE 480

Query: 481 FGFVVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAAD 540
           FGFVVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKY+TEDKAA+
Sbjct: 481 FGFVVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYETEDKAAE 540

Query: 541 TVNFDATEPVVIVGFGQMGQVLANFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSRKLG 600
           TVNFDA+EPVVIVGFGQMGQVLANFLS PL SG DG+TPGWPYVAFDIDLSVVKTSRKLG
Sbjct: 541 TVNFDASEPVVIVGFGQMGQVLANFLSAPLVSGGDGDTPGWPYVAFDIDLSVVKTSRKLG 600

Query: 601 FPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVH 660
           FPVLYGDGSRPAVLQSAGISSPKAVMVM+TEKK TI+AVQ+LRLAFPAIPIY RAKDVVH
Sbjct: 601 FPVLYGDGSRPAVLQSAGISSPKAVMVMYTEKKTTIEAVQRLRLAFPAIPIYGRAKDVVH 660

Query: 661 LLDLKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKS 720
           LLDLK AGATDAILEDAETSLQLGSKLLKGLGVMSDQVSF+SQ+VR+SMEIQAQDALDKS
Sbjct: 661 LLDLKNAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFISQIVRDSMEIQAQDALDKS 720

Query: 721 NEQELEIMKPLQIRVKDSIES--------PESELSRLNREDKTQILNGKEVDQMKQGTVF 780
           +EQELEIMKP+QIRVKDS+ES        PE  LSRLN+ED + ILNGKE++Q KQ  VF
Sbjct: 721 DEQELEIMKPMQIRVKDSLESGLPSLSTSPEDNLSRLNQEDNSLILNGKEMEQRKQDAVF 780

Query: 781 QKPEDLDGNGVLYCELDTENNLFEQNVVEPPT-HITATDGL 809
           QK EDLDG GVLYCELD+ENN  +QNV EPPT  I ATD +
Sbjct: 781 QKAEDLDGKGVLYCELDSENNFLDQNVAEPPTPFIAATDEV 821

BLAST of MELO3C018570.jh1 vs. TAIR 10
Match: AT4G04850.2 (K+ efflux antiporter 3 )

HSP 1 Score: 939.5 bits (2427), Expect = 1.8e-273
Identity = 523/765 (68.37%), Postives = 604/765 (78.95%), Query Frame = 0

Query: 1   MSFGSMLEPVSCCQSSQSQIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC 60
           M+  +ML  +SCC S +   Y  VKQ        H+V          H   + K +VP  
Sbjct: 1   MAISTMLGSISCCPSPKG--YEMVKQ--------HSVRL-------KHCVFTVKSSVP-- 60

Query: 61  TFNHWRNDYSLVPVLFHNGATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDV 120
            ++   ND  +    F N      F    +  Y           R+ T A +DVA+AVDV
Sbjct: 61  VYSEGVND-GIKLHSFGNLVKKKVFLDTSKRFYFQGRWSESSGRRVETYAGVDVASAVDV 120

Query: 121 INDLGLDTLTFLAVTVVVVPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGI 180
           INDLG DTLTFL VTV++VP FR +KASPILGFFFAG+VLNQFG+IRN+ DVKVLSEWGI
Sbjct: 121 INDLGFDTLTFLMVTVIIVPAFRILKASPILGFFFAGVVLNQFGLIRNLTDVKVLSEWGI 180

Query: 181 LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHA 240
           LFLLFEMGLELS ARLKALA+FAFGMGLTQV+L T AFTAFELP NGA+GTKILEFLFH+
Sbjct: 181 LFLLFEMGLELSLARLKALAKFAFGMGLTQVLLCTAAFTAFELPPNGAIGTKILEFLFHS 240

Query: 241 RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPL 300
           R DLVNIRS+DEA+VIGAALSLSSSAFVLQLLAEKGEL TRFGSATLGILLLQDIAVVPL
Sbjct: 241 RPDLVNIRSIDEAVVIGAALSLSSSAFVLQLLAEKGELPTRFGSATLGILLLQDIAVVPL 300

Query: 301 LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV 360
           LVILPVLESQ++G ESIWPMLA+ES KALGGLG+LSLGGK  LRR+FEVVAE RSSEAFV
Sbjct: 301 LVILPVLESQDIGGESIWPMLAKESAKALGGLGILSLGGKFFLRRIFEVVAETRSSEAFV 360

Query: 361 ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT 420
           ALCLLTVAGTSL+TQ LGFSDTLGAFLAGA+LAETNFRTQIEADIRPFRGLLLGLFFVTT
Sbjct: 361 ALCLLTVAGTSLVTQWLGFSDTLGAFLAGALLAETNFRTQIEADIRPFRGLLLGLFFVTT 420

Query: 421 GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF 480
           GTSIDM++LFREWPNVL+LL GLI IKTLIITAIGPRVGLT QESVR+GFLLSQGGEF F
Sbjct: 421 GTSIDMEVLFREWPNVLSLLGGLIVIKTLIITAIGPRVGLTIQESVRVGFLLSQGGEFAF 480

Query: 481 VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN 540
           VVFSLANRLGVLP ELNKLLII+VVLSMALTP LN+ GRKA++F+ E+    +K  + VN
Sbjct: 481 VVFSLANRLGVLPNELNKLLIIVVVLSMALTPYLNQLGRKAADFLDERLDPGEKIGEDVN 540

Query: 541 FDATEPVVIVGFGQMGQVLANFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPV 600
           FD +E +VI+GFGQMGQVLANFLSTPL S  D +  GWPY+ FD++ +VVK SRKLGFP+
Sbjct: 541 FDVSESIVIIGFGQMGQVLANFLSTPLVS--DSDLVGWPYIGFDLNPAVVKESRKLGFPI 600

Query: 601 LYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLD 660
           LYGDGSRP+VLQSAG+SSPKA+M+M+  KK T +AVQ+LRLAFP  PIYARA+D+ HLL+
Sbjct: 601 LYGDGSRPSVLQSAGVSSPKAIMIMYKGKKRTTEAVQRLRLAFPGSPIYARAQDLPHLLE 660

Query: 661 LKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKS--- 720
           LK AGATDAILE+AETSLQLGSKLL G GVMSD VSFLS++ R+SMEIQAQ+ +  S   
Sbjct: 661 LKKAGATDAILENAETSLQLGSKLLTGFGVMSDDVSFLSKVFRDSMEIQAQEEITASETN 720

Query: 721 -------------------NEQELEIMKPLQIRVKDSIESPESEL 744
                               ++++++MKP+Q++  DS     +E+
Sbjct: 721 AGLKPMQMKASDINVVSAATQKQVQLMKPMQMKASDSNSDSAAEI 743

BLAST of MELO3C018570.jh1 vs. TAIR 10
Match: AT4G04850.1 (K+ efflux antiporter 3 )

HSP 1 Score: 839.7 bits (2168), Expect = 1.9e-243
Identity = 440/542 (81.18%), Postives = 491/542 (90.59%), Query Frame = 0

Query: 105 RLRTRAALDVAAAVDVINDLGLDTLTFLAVTVVVVPLFRKVKASPILGFFFAGIVLNQFG 164
           R+ T A +DVA+AVDVINDLG DTLTFL VTV++VP FR +KASPILGFFFAG+VLNQFG
Sbjct: 85  RVETYAGVDVASAVDVINDLGFDTLTFLMVTVIIVPAFRILKASPILGFFFAGVVLNQFG 144

Query: 165 VIRNIVDVKVLSEWGILFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELP 224
           +IRN+ DVKVLSEWGILFLLFEMGLELS ARLKALA+FAFGMGLTQV+L T AFTAFELP
Sbjct: 145 LIRNLTDVKVLSEWGILFLLFEMGLELSLARLKALAKFAFGMGLTQVLLCTAAFTAFELP 204

Query: 225 TNGAVGTKILEFLFHARSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGS 284
            NGA+GTKILEFLFH+R DLVNIRS+DEA+VIGAALSLSSSAFVLQLLAEKGEL TRFGS
Sbjct: 205 PNGAIGTKILEFLFHSRPDLVNIRSIDEAVVIGAALSLSSSAFVLQLLAEKGELPTRFGS 264

Query: 285 ATLGILLLQDIAVVPLLVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILR 344
           ATLGILLLQDIAVVPLLVILPVLESQ++G ESIWPMLA+ES KALGGLG+LSLGGK  LR
Sbjct: 265 ATLGILLLQDIAVVPLLVILPVLESQDIGGESIWPMLAKESAKALGGLGILSLGGKFFLR 324

Query: 345 RVFEVVAEARSSEAFVALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEAD 404
           R+FEVVAE RSSEAFVALCLLTVAGTSL+TQ LGFSDTLGAFLAGA+LAETNFRTQIEAD
Sbjct: 325 RIFEVVAETRSSEAFVALCLLTVAGTSLVTQWLGFSDTLGAFLAGALLAETNFRTQIEAD 384

Query: 405 IRPFRGLLLGLFFVTTGTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQE 464
           IRPFRGLLLGLFFVTTGTSIDM++LFREWPNVL+LL GLI IKTLIITAIGPRVGLT QE
Sbjct: 385 IRPFRGLLLGLFFVTTGTSIDMEVLFREWPNVLSLLGGLIVIKTLIITAIGPRVGLTIQE 444

Query: 465 SVRIGFLLSQGGEFGFVVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEF 524
           SVR+GFLLSQGGEF FVVFSLANRLGVLP ELNKLLII+VVLSMALTP LN+ GRKA++F
Sbjct: 445 SVRVGFLLSQGGEFAFVVFSLANRLGVLPNELNKLLIIVVVLSMALTPYLNQLGRKAADF 504

Query: 525 ISEKYKTEDKAADTVNFDATEPVVIVGFGQMGQVLANFLSTPLASGIDGNTPGWPYVAFD 584
           + E+    +K  + VNFD +E +VI+GFGQMGQVLANFLSTPL S  D +  GWPY+ FD
Sbjct: 505 LDERLDPGEKIGEDVNFDVSESIVIIGFGQMGQVLANFLSTPLVS--DSDLVGWPYIGFD 564

Query: 585 IDLSVVKTSRKLGFPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFP 644
           ++ +VVK SRKLGFP+LYGDGSRP+VLQSAG+SSPKA+M+M+  KK T +AVQ+LRLAFP
Sbjct: 565 LNPAVVKESRKLGFPILYGDGSRPSVLQSAGVSSPKAIMIMYKGKKRTTEAVQRLRLAFP 624

Query: 645 AI 647
            +
Sbjct: 625 GV 624

BLAST of MELO3C018570.jh1 vs. TAIR 10
Match: AT4G00630.1 (K+ efflux antiporter 2 )

HSP 1 Score: 339.3 bits (869), Expect = 8.2e-93
Identity = 216/571 (37.83%), Postives = 323/571 (56.57%), Query Frame = 0

Query: 126  LDTLTFLAVTVVVVPLFRKVK-ASPILGFFFAGIVLNQFG--VIRNIVDVKVLSEWGILF 185
            LD L  L  +V+ VPLF+K+   SP+LG+  AGI++  +G  +IRN+   K ++E+G++F
Sbjct: 570  LDVLWLLLASVIFVPLFQKIPGGSPVLGYLAAGILIGPYGLSIIRNVHGTKAIAEFGVVF 629

Query: 186  LLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARS 245
            LLF +GLELS  RL ++ ++ FG+G  QV+++            G  G            
Sbjct: 630  LLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAAVIGLITHYVAGQAG------------ 689

Query: 246  DLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPLLV 305
                      AIVIG  L+LSS+A VLQ+L E+GE  +R G AT  +LL QD+AVV LL+
Sbjct: 690  --------PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLI 749

Query: 306  ILPVLESQN----LGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEA 365
            ++P++   +    +G ++I   L   ++KA   +  +  GG+L+LR +++ +AE R++E 
Sbjct: 750  LIPLISPNSSKGGIGFQAIAEALGLAAIKAAVAITGIIAGGRLLLRPIYKQIAENRNAEI 809

Query: 366  FVALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFV 425
            F A  LL + GTSL+T + G S  LGAFLAG +LAET F  Q+E+DI P+RGLLLGLFF+
Sbjct: 810  FSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFM 869

Query: 426  TTGTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEF 485
            T G SID +LL   +P ++  L  L+  KT+++  IG   G++   +VR+G LL+ GGEF
Sbjct: 870  TVGMSIDPKLLLANFPLIMGTLGLLLVGKTILVVIIGKLFGISIISAVRVGLLLAPGGEF 929

Query: 486  GFVVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAA-- 545
             FV F  A   G++  +L+ LL ++V +SMALTP L   G    + I+ +++ +D  +  
Sbjct: 930  AFVAFGEAVNQGIMTPQLSSLLFLVVGISMALTPWLAAGG----QLIASRFELQDVRSLL 989

Query: 546  --DTVNFDATEPVVIVGFGQMGQVLANFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSR 605
              ++   D    ++I GFG++GQ++A  LS  L           P+VA D+    V   R
Sbjct: 990  PVESETDDLQGHIIICGFGRIGQIIAQLLSERLI----------PFVALDVSSDRVAIGR 1049

Query: 606  KLGFPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKD 665
             L  PV +GD     VL   G     A  +      A    V  L   FP +  + RA D
Sbjct: 1050 SLDLPVYFGDAGSREVLHKIGADRACAAAIALDTPGANYRCVWALSKYFPNVKTFVRAHD 1106

Query: 666  VVHLLDLKTAGATDAILEDAETSLQLGSKLL 686
            V H L+L+ AGAT  + E  E SLQL + +L
Sbjct: 1110 VDHGLNLEKAGATAVVPETLEPSLQLAAAVL 1106

BLAST of MELO3C018570.jh1 vs. TAIR 10
Match: AT1G01790.1 (K+ efflux antiporter 1 )

HSP 1 Score: 335.5 bits (859), Expect = 1.2e-91
Identity = 212/570 (37.19%), Postives = 323/570 (56.67%), Query Frame = 0

Query: 127  DTLTFLAVTVVVVPLFRKVK-ASPILGFFFAGIVLNQFG--VIRNIVDVKVLSEWGILFL 186
            D L  L  +V+ VPLF+K+   SP+LG+  AGI++  +G  +IRN+   + ++E+G++FL
Sbjct: 591  DFLWLLLASVIFVPLFQKIPGGSPVLGYLAAGILIGPYGLSIIRNVHGTRAIAEFGVVFL 650

Query: 187  LFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARSD 246
            LF +GLELS  RL ++ ++ FG+G  QV+++            G  G             
Sbjct: 651  LFNIGLELSVERLSSMKKYVFGLGSAQVLVTAAVVGLLAHYVAGQAG------------- 710

Query: 247  LVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPLLVI 306
                     AIVIG  L+LSS+A VLQ+L E+GE  +R G A+  +LL QD+AVV LL++
Sbjct: 711  -------PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRASFSVLLFQDLAVVVLLIL 770

Query: 307  LPVLESQN----LGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAF 366
            +P++   +    +G ++I   L   ++KA   +  +  GG+L+LR +++ +AE R++E F
Sbjct: 771  IPLISPNSSKGGIGFQAIAEALGLAAVKAAVAITAIIAGGRLLLRPIYKQIAENRNAEIF 830

Query: 367  VALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVT 426
             A  LL + GTSL+T + G S  LGAFLAG +LAET F  Q+E+DI P+RGLLLGLFF+T
Sbjct: 831  SANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMT 890

Query: 427  TGTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFG 486
             G SID +LL   +P ++  L  LI  KT+++  +G   G++   ++R+G LL+ GGEF 
Sbjct: 891  VGMSIDPKLLLSNFPVIVGTLGLLIVGKTMLVVIMGKLFGISIISAIRVGLLLAPGGEFA 950

Query: 487  FVVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAA--- 546
            FV F  A   G++  +L+ LL ++V +SMA+TP L   G    + I+ +++  D  +   
Sbjct: 951  FVAFGEAVNQGIMSPQLSSLLFLVVGISMAITPWLAAGG----QLIASRFELHDVRSLLP 1010

Query: 547  -DTVNFDATEPVVIVGFGQMGQVLANFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSRK 606
             ++   D    ++I GFG++GQ++A  LS  L           P+VA D+    V   R 
Sbjct: 1011 VESETDDLQGHIIICGFGRVGQIIAQLLSERLI----------PFVALDVSSDRVTIGRS 1070

Query: 607  LGFPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDV 666
            L  PV +GD     VL   G     A +V      A    V  L   +P +  + RA DV
Sbjct: 1071 LDLPVYFGDAGSKEVLHKIGAGRACAAVVALDAPGANYRCVWALSKFYPNVKTFVRAHDV 1126

Query: 667  VHLLDLKTAGATDAILEDAETSLQLGSKLL 686
            VH L+L+ AGAT  + E  E SLQL + +L
Sbjct: 1131 VHGLNLEKAGATAVVPETLEPSLQLAAAVL 1126

BLAST of MELO3C018570.jh1 vs. TAIR 10
Match: AT4G00630.2 (K+ efflux antiporter 2 )

HSP 1 Score: 330.9 bits (847), Expect = 2.9e-90
Identity = 216/582 (37.11%), Postives = 323/582 (55.50%), Query Frame = 0

Query: 126  LDTLTFLAVTVVVVPLFRKVK-ASPILGFFFAGIVLNQFG--VIRNIVDVKVLSEWGILF 185
            LD L  L  +V+ VPLF+K+   SP+LG+  AGI++  +G  +IRN+   K ++E+G++F
Sbjct: 570  LDVLWLLLASVIFVPLFQKIPGGSPVLGYLAAGILIGPYGLSIIRNVHGTKAIAEFGVVF 629

Query: 186  LLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARS 245
            LLF +GLELS  RL ++ ++ FG+G  QV+++            G  G            
Sbjct: 630  LLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAAVIGLITHYVAGQAG------------ 689

Query: 246  DLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPLLV 305
                      AIVIG  L+LSS+A VLQ+L E+GE  +R G AT  +LL QD+AVV LL+
Sbjct: 690  --------PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLI 749

Query: 306  ILPVLESQN----LGTESIWPMLAQESLKALGGLGLLSLGGKL-----------ILRRVF 365
            ++P++   +    +G ++I   L   ++KA   +  +  GG+L           +LR ++
Sbjct: 750  LIPLISPNSSKGGIGFQAIAEALGLAAIKAAVAITGIIAGGRLFYNALIFWYTQLLRPIY 809

Query: 366  EVVAEARSSEAFVALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRP 425
            + +AE R++E F A  LL + GTSL+T + G S  LGAFLAG +LAET F  Q+E+DI P
Sbjct: 810  KQIAENRNAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAP 869

Query: 426  FRGLLLGLFFVTTGTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVR 485
            +RGLLLGLFF+T G SID +LL   +P ++  L  L+  KT+++  IG   G++   +VR
Sbjct: 870  YRGLLLGLFFMTVGMSIDPKLLLANFPLIMGTLGLLLVGKTILVVIIGKLFGISIISAVR 929

Query: 486  IGFLLSQGGEFGFVVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISE 545
            +G LL+ GGEF FV F  A   G++  +L+ LL ++V +SMALTP L   G    + I+ 
Sbjct: 930  VGLLLAPGGEFAFVAFGEAVNQGIMTPQLSSLLFLVVGISMALTPWLAAGG----QLIAS 989

Query: 546  KYKTEDKAA----DTVNFDATEPVVIVGFGQMGQVLANFLSTPLASGIDGNTPGWPYVAF 605
            +++ +D  +    ++   D    ++I GFG++GQ++A  LS  L           P+VA 
Sbjct: 990  RFELQDVRSLLPVESETDDLQGHIIICGFGRIGQIIAQLLSERLI----------PFVAL 1049

Query: 606  DIDLSVVKTSRKLGFPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAF 665
            D+    V   R L  PV +GD     VL   G     A  +      A    V  L   F
Sbjct: 1050 DVSSDRVAIGRSLDLPVYFGDAGSREVLHKIGADRACAAAIALDTPGANYRCVWALSKYF 1109

Query: 666  PAIPIYARAKDVVHLLDLKTAGATDAILEDAETSLQLGSKLL 686
            P +  + RA DV H L+L+ AGAT  + E  E SLQL + +L
Sbjct: 1110 PNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSLQLAAAVL 1117