MELO3C017853.jh1 (gene) Melon (Harukei-3) v1.41

Overview
NameMELO3C017853.jh1
Typegene
OrganismCucumis melo var. reticulatus cv. Harukei-3 (Melon (Harukei-3) v1.41)
Description3-isopropylmalate dehydrogenase
Locationchr07: 26310736 .. 26314936 (+)
RNA-Seq ExpressionMELO3C017853.jh1
SyntenyMELO3C017853.jh1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonstart_codonCDSpolypeptidestop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCATTTCCACACTTGGAGACTTTCAGCTGACTTCATCCATGGCGGCTGCTTTGCATCTCAACGCTAAGCCTTTCAATCTTTGTTCCCCATCTATTAGGCTTCCAAAGTCATCGCCGTCTCCTTTCCGAGTCAAGTGCTCTGCCGGGACTTCCCCTTCCAGGCGCTATAATATCGCCCTGCTTCCCGGCGATGGCATTGGCCCCGAAGTTATCGCCGTCGCCAAGGATGTTCTCAACTTTGCTGCTTCTCTTGAAGGTCTCTCTCTCTCTCTCTCTCTCTACTCCCTCTCTGTTTCTTCCCCTTCCATTTGGGTTTATGTTTTGTTGGATGGATACTGAATTCTTTGGTGAGATTGTACATTTCGTATTTGTTTTTGATGAGATTGGATTTTGTTTTCGATTAGGGATTGGATTCGATTACAAAGAGATGCCTGTGGGTGGAGCTGCGCTTGATTTAACCGGGGTTCCATTGCCAGATGAGACGCTTTTAGCAGCAAAGGAGTCTGATGCCATTCTATTTGGGGCAATTGGAGGGTAACTCTACTTATAGCTTATGCTATCACATTTGGTCCTTTTTTTTTTCTTTTTTTATTTAAATGGTGTGGTTTGTTGAATAATCTCTTTGTTCGTTCCGTTTACCAAGTTACAAATGGGATGATAATCCAAAACATTTGAAGCCAGAGACTGGGTTGCTTCAACTTCGGAAAGGTCTTGGAGTGTTTGCTAATTTGCGGCCTGCAACCGTTTTTCCCCAGGTAAACTTCTACAATATCCTCTCCCTACTACACCAAACTATTTACAAAGATATTGCAAGCTCAAGGTTCATTTTTACTTTTTGGCCTGTGGTCAGTTATATTCCGTATAAACAAGATTGGTATCTTGTCAAGTTCCTGATGCAACGAATAGATGTTTTTGGTTATTTGAAGACTGGGGAAAGTTGCTTGGTTTGATACCCTATAGATAGTCTTGTTTTATGTTAAATTTTAGGCTAGCCTTATGCTGACTCGATAGTCAATATGTTCATAACGACTATGGGTTATTGTTTGAAGCCCTATAGATGGCCTCGTTTCCTGTTAGTTTAGACATCTGAATTAATAAAACATGTTATGATTTAATGTTCGTAATCACCAATGGGTTAGCCTATTAGTCTGTAAGGTTATGTAAATAATAAAATGGTGTGAAGAAATAGTTCCAACCGTGATGGCCACCTACTCAAGATTTAATACCTTATGAGTTACCTTAGCAATCAAATGTAGTAGGACTAAACAGTTTTTCCAAGAAAATAGTTGAGGTATGTGCAAGTTGATTAAACTCTCGTGGATATCTAAAAATAAGAGAAATTTTGCTGGTAAGTTCAGTGGTCAACTTGTCAAGACTTTCAATGGCTTGCATTATATTTAGAAGCGCAGGGTGGAACTTCAAATTTCATGGGTCCTAAATGAAAACATGAAAGAATTTTCCCTATTAACATTTTTAATTGAGATTAATTCATGTGCCTGTGAGTTTATGAAGATATACCAGCGAGTATGAGGAAGAAGCTTATATGAGGGACTCTATTGCCTATTGATTACTCCGTTTCTTACTTCTTCAGTTGGTGGATGCTTCGACTTTGAAGAGAGAGGTTGCTGAAGGTGTTGATCTCATGGTCGTAAGGGAACTCACCGGAGGTAAATAATTTTCCCCTCCCAAATGAAATGAGACTGCATGTCTTGATTTCCATCCATCTCTCAATTTTGGGGTTTTGAATAAGGATCCCATGATTTCTTCCACCTGAATTAGGTATTTATTTTGGGCAACCAAGAGGTTTTAAAACCAGTGAAAATGGGGAAGAAATTGGATTTAATACTGAAGTGTATGCAGCACAGGAGGTAATTTATTTATTTTTAATTCCACTTCCTTGTGAGTTTGCATATAGAGTATCCACGACCTTTTATTCTTATTCGACATGCTCTCATCTTTCATCATAAAATCATCAATAACATTGAGATCAGCATTCCTCGAATTCAAAATTTTCACGCTGATTTACAAATTCATTCTTCAAAGTGATCATTCACGCTTGCATGTAAAAGTTATACCTTTCTTGAAATTAAGATTACATGCTTAATTTGTTTCTATATCTATAATAATCCTCTTTTATTTTATATACTATATTGTATCTATTGTTACAATTATGTTTCGGTTTGTTAATGCTTCTATTTCAGATTGATCGTGTTGCTCGAGTGGCATTTAACAATGCTCGGAAGCGTCAAGGAAGAGTTTGTTCTGTAGACAAAGCAAATGTGTTGGAGGTGCTTAAAACTTTGATAATTTCTTTTCTTGATTGATTGATGTTGTATCCATCGTTATTCATAAGAACAACCTTTTCATCCCTTATCAAGCATTTCCTACTTAACGAAGGATGGTGGAAGCTTTCTATGGAATCTGTTAGTGGAGGAAGTTTGGGATAATATCGTGCCTCTTTCTTCTTCTTGGTCGTCTTTGTCAACTTTTTAAAAATTTTAGTTCTCTGTATTATTGCAAATTTTGCATCGTGCCGATCAGAAAATCTTTCTCTAGCCTTAAATTAGGGCTTAGCCTTTCTCATCTATTTCATTGATCTCCACTGAAAAAGTTGTTTCTCTTAGAAAAGGTTTTTAGGAAGTTCGAGAAGACAACCTCAGACCATTCCTTGATTTTGCTGGAAATAGGTTGAATTTTGAGAATGTGTTGCTGTGATCTCCAGATTTCTTCTAAGTTTTGCTGAAGATTCATTCAGTTCAGGATTTCCCTACTTTATTAAAATATAGTCATACATTTCAGCTCAGTATGTTATGTATGGTCCATGCATGTAAAAGTGTGGTCATGTGTGCTGTAGCAACCTTACACTTGAACTGTCTATTTTGTAGGCATCGATACTTTGGAGGAAGAGAGTGACAGCAATAGCTGCAGAATACCCTGATGTCGAACTCTCGCACATGTACGTTGATAACGCTGCGATGCAACTAATTCGGGAACCAAAACAGGTGTGCATTGAAAAGTAGTCTTATTTCCATAGTAGGAGCTATTAAAGTGGCTTCATAGCTCCTAGTTGATTTTTGTTTGGACTGAGTGTGGGAATTTGCAGTTTGATACAATTTTGACAAACAACATATTTGGTGATATTTTATCGGATGCTGCATCCATGATTACTGGAAGTATTGGGATGCTTCCATCTGCAAGTTTACGTGAATCGGTAATCAAAATTTTTCGCTTACATTTGTATCGTGGATTTTCTATTAAGTTGTGCATTTCTCTTATGATCTAATCTTCTTCGATCCCTTCTAATGAACTTAACCGTTTGCACAGGGACCTGGACTTTTCGAACCTATACATGGGTCAGCTCCTGATATTGCTGGACAGGTTTGTGTTTTTAGAAGATCAAAATCATATCAGTAAGGATACAAAGTTTTATGATATAAAATGACGTGGCTTTATCTATAGGATAAAGCAAATCCACTGGCAACAGTTCTCAGTGCTGCTATGCTTCTAAAGTATGGTCTCGGTGAAGAAAATGCTGCCAAAAGAATTGAGGCTGCAGTTTTGGATACTCTGGACAAAGGATTTAGAACCGGAGACATATACTCGGCTGGATGTGTACGTATTTTCTCGTTCTCTCTTTAGTCTTTGGATACTCTGTCCAGTAGTAATCAAATGACTTGAACCAATATATTTTAAATATCATGAAAATGAATAATGTTGCTAGTTTTTGTCCCATATTGATCAAATCAAGCTGGTTTTATTTGTTCGATCAACTCTTTTAGGGTTCAAATTACATCCCTTTTATGATCAAATGTATTGCGGAGCTAGGATGTTCTGTTTTACTGCCAATTTTGATAACTATCCTTAGTACTTCCTCTTTTTGGTCATCATTCTAACGATTTTCTTAACTGCACAGAAACGAGTCGGATGCATAGAGATGGGTGAGGAGCTGTTAAAGTCTATAGAGTCCCATAAACTTGCTACAGTTTGAGTCGAACGTGGGCGAGGGATCCTTTGTTTGCTTCATTGCTTTGCAAATGCGCTCTGATGTCAGTGTCCAACCTTGAGGTTCTCTCAATATATTTATGAACAAGTTGAGTTTAAGGTCTTCTTTTTGCGTTGGAACTATAAATAACTGTCATCTTAGGTTGTAACATTCTGACATTTTTTATAATAGGAAAATAAAATTAATTTGTCAGGCCAA

mRNA sequence

CCCATTTCCACACTTGGAGACTTTCAGCTGACTTCATCCATGGCGGCTGCTTTGCATCTCAACGCTAAGCCTTTCAATCTTTGTTCCCCATCTATTAGGCTTCCAAAGTCATCGCCGTCTCCTTTCCGAGTCAAGTGCTCTGCCGGGACTTCCCCTTCCAGGCGCTATAATATCGCCCTGCTTCCCGGCGATGGCATTGGCCCCGAAGTTATCGCCGTCGCCAAGGATGTTCTCAACTTTGCTGCTTCTCTTGAAGGGATTGGATTCGATTACAAAGAGATGCCTGTGGGTGGAGCTGCGCTTGATTTAACCGGGGTTCCATTGCCAGATGAGACGCTTTTAGCAGCAAAGGAGTCTGATGCCATTCTATTTGGGGCAATTGGAGGTTACAAATGGGATGATAATCCAAAACATTTGAAGCCAGAGACTGGGTTGCTTCAACTTCGGAAAGGTCTTGGAGTGTTTGCTAATTTGCGGCCTGCAACCGTTTTTCCCCAGTTGGTGGATGCTTCGACTTTGAAGAGAGAGGTTGCTGAAGGTGTTGATCTCATGGTCGTAAGGGAACTCACCGGAGGTATTTATTTTGGGCAACCAAGAGGTTTTAAAACCAGTGAAAATGGGGAAGAAATTGGATTTAATACTGAAGTGTATGCAGCACAGGAGATTGATCGTGTTGCTCGAGTGGCATTTAACAATGCTCGGAAGCGTCAAGGAAGAGTTTGTTCTGTAGACAAAGCAAATGTGTTGGAGGCATCGATACTTTGGAGGAAGAGAGTGACAGCAATAGCTGCAGAATACCCTGATGTCGAACTCTCGCACATGTACGTTGATAACGCTGCGATGCAACTAATTCGGGAACCAAAACAGTTTGATACAATTTTGACAAACAACATATTTGGTGATATTTTATCGGATGCTGCATCCATGATTACTGGAAGTATTGGGATGCTTCCATCTGCAAGTTTACGTGAATCGGGACCTGGACTTTTCGAACCTATACATGGGTCAGCTCCTGATATTGCTGGACAGGATAAAGCAAATCCACTGGCAACAGTTCTCAGTGCTGCTATGCTTCTAAAGTATGGTCTCGGTGAAGAAAATGCTGCCAAAAGAATTGAGGCTGCAGTTTTGGATACTCTGGACAAAGGATTTAGAACCGGAGACATATACTCGGCTGGATGTAAACGAGTCGGATGCATAGAGATGGGTGAGGAGCTGTTAAAGTCTATAGAGTCCCATAAACTTGCTACAGTTTGAGTCGAACGTGGGCGAGGGATCCTTTGTTTGCTTCATTGCTTTGCAAATGCGCTCTGATGTCAGTGTCCAACCTTGAGGTTCTCTCAATATATTTATGAACAAGTTGAGTTTAAGGTCTTCTTTTTGCGTTGGAACTATAAATAACTGTCATCTTAGGTTGTAACATTCTGACATTTTTTATAATAGGAAAATAAAATTAATTTGTCAGGCCAA

Coding sequence (CDS)

ATGGCGGCTGCTTTGCATCTCAACGCTAAGCCTTTCAATCTTTGTTCCCCATCTATTAGGCTTCCAAAGTCATCGCCGTCTCCTTTCCGAGTCAAGTGCTCTGCCGGGACTTCCCCTTCCAGGCGCTATAATATCGCCCTGCTTCCCGGCGATGGCATTGGCCCCGAAGTTATCGCCGTCGCCAAGGATGTTCTCAACTTTGCTGCTTCTCTTGAAGGGATTGGATTCGATTACAAAGAGATGCCTGTGGGTGGAGCTGCGCTTGATTTAACCGGGGTTCCATTGCCAGATGAGACGCTTTTAGCAGCAAAGGAGTCTGATGCCATTCTATTTGGGGCAATTGGAGGTTACAAATGGGATGATAATCCAAAACATTTGAAGCCAGAGACTGGGTTGCTTCAACTTCGGAAAGGTCTTGGAGTGTTTGCTAATTTGCGGCCTGCAACCGTTTTTCCCCAGTTGGTGGATGCTTCGACTTTGAAGAGAGAGGTTGCTGAAGGTGTTGATCTCATGGTCGTAAGGGAACTCACCGGAGGTATTTATTTTGGGCAACCAAGAGGTTTTAAAACCAGTGAAAATGGGGAAGAAATTGGATTTAATACTGAAGTGTATGCAGCACAGGAGATTGATCGTGTTGCTCGAGTGGCATTTAACAATGCTCGGAAGCGTCAAGGAAGAGTTTGTTCTGTAGACAAAGCAAATGTGTTGGAGGCATCGATACTTTGGAGGAAGAGAGTGACAGCAATAGCTGCAGAATACCCTGATGTCGAACTCTCGCACATGTACGTTGATAACGCTGCGATGCAACTAATTCGGGAACCAAAACAGTTTGATACAATTTTGACAAACAACATATTTGGTGATATTTTATCGGATGCTGCATCCATGATTACTGGAAGTATTGGGATGCTTCCATCTGCAAGTTTACGTGAATCGGGACCTGGACTTTTCGAACCTATACATGGGTCAGCTCCTGATATTGCTGGACAGGATAAAGCAAATCCACTGGCAACAGTTCTCAGTGCTGCTATGCTTCTAAAGTATGGTCTCGGTGAAGAAAATGCTGCCAAAAGAATTGAGGCTGCAGTTTTGGATACTCTGGACAAAGGATTTAGAACCGGAGACATATACTCGGCTGGATGTAAACGAGTCGGATGCATAGAGATGGGTGAGGAGCTGTTAAAGTCTATAGAGTCCCATAAACTTGCTACAGTTTGA

Protein sequence

MAAALHLNAKPFNLCSPSIRLPKSSPSPFRVKCSAGTSPSRRYNIALLPGDGIGPEVIAVAKDVLNFAASLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKESDAILFGAIGGYKWDDNPKHLKPETGLLQLRKGLGVFANLRPATVFPQLVDASTLKREVAEGVDLMVVRELTGGIYFGQPRGFKTSENGEEIGFNTEVYAAQEIDRVARVAFNNARKRQGRVCSVDKANVLEASILWRKRVTAIAAEYPDVELSHMYVDNAAMQLIREPKQFDTILTNNIFGDILSDAASMITGSIGMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGLGEENAAKRIEAAVLDTLDKGFRTGDIYSAGCKRVGCIEMGEELLKSIESHKLATV
Homology
BLAST of MELO3C017853.jh1 vs. NCBI nr
Match: XP_008454199.1 (PREDICTED: 3-isopropylmalate dehydrogenase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 798 bits (2061), Expect = 3.48e-291
Identity = 405/405 (100.00%), Postives = 405/405 (100.00%), Query Frame = 0

Query: 1   MAAALHLNAKPFNLCSPSIRLPKSSPSPFRVKCSAGTSPSRRYNIALLPGDGIGPEVIAV 60
           MAAALHLNAKPFNLCSPSIRLPKSSPSPFRVKCSAGTSPSRRYNIALLPGDGIGPEVIAV
Sbjct: 1   MAAALHLNAKPFNLCSPSIRLPKSSPSPFRVKCSAGTSPSRRYNIALLPGDGIGPEVIAV 60

Query: 61  AKDVLNFAASLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKESDAILFGAIGGYKWD 120
           AKDVLNFAASLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKESDAILFGAIGGYKWD
Sbjct: 61  AKDVLNFAASLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKESDAILFGAIGGYKWD 120

Query: 121 DNPKHLKPETGLLQLRKGLGVFANLRPATVFPQLVDASTLKREVAEGVDLMVVRELTGGI 180
           DNPKHLKPETGLLQLRKGLGVFANLRPATVFPQLVDASTLKREVAEGVDLMVVRELTGGI
Sbjct: 121 DNPKHLKPETGLLQLRKGLGVFANLRPATVFPQLVDASTLKREVAEGVDLMVVRELTGGI 180

Query: 181 YFGQPRGFKTSENGEEIGFNTEVYAAQEIDRVARVAFNNARKRQGRVCSVDKANVLEASI 240
           YFGQPRGFKTSENGEEIGFNTEVYAAQEIDRVARVAFNNARKRQGRVCSVDKANVLEASI
Sbjct: 181 YFGQPRGFKTSENGEEIGFNTEVYAAQEIDRVARVAFNNARKRQGRVCSVDKANVLEASI 240

Query: 241 LWRKRVTAIAAEYPDVELSHMYVDNAAMQLIREPKQFDTILTNNIFGDILSDAASMITGS 300
           LWRKRVTAIAAEYPDVELSHMYVDNAAMQLIREPKQFDTILTNNIFGDILSDAASMITGS
Sbjct: 241 LWRKRVTAIAAEYPDVELSHMYVDNAAMQLIREPKQFDTILTNNIFGDILSDAASMITGS 300

Query: 301 IGMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGLGEENAAKRIE 360
           IGMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGLGEENAAKRIE
Sbjct: 301 IGMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGLGEENAAKRIE 360

Query: 361 AAVLDTLDKGFRTGDIYSAGCKRVGCIEMGEELLKSIESHKLATV 405
           AAVLDTLDKGFRTGDIYSAGCKRVGCIEMGEELLKSIESHKLATV
Sbjct: 361 AAVLDTLDKGFRTGDIYSAGCKRVGCIEMGEELLKSIESHKLATV 405

BLAST of MELO3C017853.jh1 vs. NCBI nr
Match: XP_011652988.1 (3-isopropylmalate dehydrogenase, chloroplastic [Cucumis sativus] >KGN52920.1 hypothetical protein Csa_015202 [Cucumis sativus])

HSP 1 Score: 778 bits (2008), Expect = 4.16e-283
Identity = 394/405 (97.28%), Postives = 399/405 (98.52%), Query Frame = 0

Query: 1   MAAALHLNAKPFNLCSPSIRLPKSSPSPFRVKCSAGTSPSRRYNIALLPGDGIGPEVIAV 60
           MAAALHLNAKPF+LCSPSI LPK SPS FRVKCSAGTSPSRRYNIALLPGDGIGPEVIAV
Sbjct: 1   MAAALHLNAKPFSLCSPSISLPKISPSSFRVKCSAGTSPSRRYNIALLPGDGIGPEVIAV 60

Query: 61  AKDVLNFAASLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKESDAILFGAIGGYKWD 120
           AKDVLNFAASLEGIGFDYKEMP+GGAALDLTGVPLPDETLLAAK+SDAILFGAIGGYKWD
Sbjct: 61  AKDVLNFAASLEGIGFDYKEMPMGGAALDLTGVPLPDETLLAAKDSDAILFGAIGGYKWD 120

Query: 121 DNPKHLKPETGLLQLRKGLGVFANLRPATVFPQLVDASTLKREVAEGVDLMVVRELTGGI 180
           DNPKHLKPETGLLQLRKGLGVFANLRPA VFPQLV+ASTLKREVAEGVDLMVVRELTGGI
Sbjct: 121 DNPKHLKPETGLLQLRKGLGVFANLRPAIVFPQLVEASTLKREVAEGVDLMVVRELTGGI 180

Query: 181 YFGQPRGFKTSENGEEIGFNTEVYAAQEIDRVARVAFNNARKRQGRVCSVDKANVLEASI 240
           YFGQPRGFKT ENGEEIGFNTEVYAAQEIDRVARVAFNNARKRQGRVCSVDKANVLEASI
Sbjct: 181 YFGQPRGFKTGENGEEIGFNTEVYAAQEIDRVARVAFNNARKRQGRVCSVDKANVLEASI 240

Query: 241 LWRKRVTAIAAEYPDVELSHMYVDNAAMQLIREPKQFDTILTNNIFGDILSDAASMITGS 300
           LWRKRVTAIAAEYPD+ELSHMYVDNAAMQLIREPKQFDTILTNNIFGDILSDAASMITGS
Sbjct: 241 LWRKRVTAIAAEYPDIELSHMYVDNAAMQLIREPKQFDTILTNNIFGDILSDAASMITGS 300

Query: 301 IGMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGLGEENAAKRIE 360
           IGMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGLGEENAAKRIE
Sbjct: 301 IGMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGLGEENAAKRIE 360

Query: 361 AAVLDTLDKGFRTGDIYSAGCKRVGCIEMGEELLKSIESHKLATV 405
           AAVLDTLDKGFRTGDIYSAGCKRVGC EMGEELLKSIESHKLATV
Sbjct: 361 AAVLDTLDKGFRTGDIYSAGCKRVGCKEMGEELLKSIESHKLATV 405

BLAST of MELO3C017853.jh1 vs. NCBI nr
Match: XP_038893808.1 (3-isopropylmalate dehydrogenase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 759 bits (1960), Expect = 8.60e-276
Identity = 386/405 (95.31%), Postives = 392/405 (96.79%), Query Frame = 0

Query: 1   MAAALHLNAKPFNLCSPSIRLPKSSPSPFRVKCSAGTSPSRRYNIALLPGDGIGPEVIAV 60
           MAAALHLN KPFN CS SI L K SP+ FRV+CSAGTSPSRRYNIALLPGDGIGPEV+AV
Sbjct: 1   MAAALHLNTKPFNFCSRSINLSKPSPTLFRVRCSAGTSPSRRYNIALLPGDGIGPEVLAV 60

Query: 61  AKDVLNFAASLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKESDAILFGAIGGYKWD 120
           AKDVLN AASLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKESDAILFGAIGGYKWD
Sbjct: 61  AKDVLNLAASLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKESDAILFGAIGGYKWD 120

Query: 121 DNPKHLKPETGLLQLRKGLGVFANLRPATVFPQLVDASTLKREVAEGVDLMVVRELTGGI 180
           DNPKHLKPETGLLQLRKGLGVFANLRPATVFPQLVDASTLKREVAEGVDLMVVRELTGGI
Sbjct: 121 DNPKHLKPETGLLQLRKGLGVFANLRPATVFPQLVDASTLKREVAEGVDLMVVRELTGGI 180

Query: 181 YFGQPRGFKTSENGEEIGFNTEVYAAQEIDRVARVAFNNARKRQGRVCSVDKANVLEASI 240
           YFGQPRGFKTSENGEEIGFNTEVYAAQEIDRVARVAF NA+KRQGRVCSVDKANVLEASI
Sbjct: 181 YFGQPRGFKTSENGEEIGFNTEVYAAQEIDRVARVAFKNAQKRQGRVCSVDKANVLEASI 240

Query: 241 LWRKRVTAIAAEYPDVELSHMYVDNAAMQLIREPKQFDTILTNNIFGDILSDAASMITGS 300
           LWRKRVTAIAAE+PDVELSHMYVDNAAMQLIREPKQFDTILTNNIFGDILSDAASMITGS
Sbjct: 241 LWRKRVTAIAAEFPDVELSHMYVDNAAMQLIREPKQFDTILTNNIFGDILSDAASMITGS 300

Query: 301 IGMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGLGEENAAKRIE 360
           IGMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGLGEENAAKRIE
Sbjct: 301 IGMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGLGEENAAKRIE 360

Query: 361 AAVLDTLDKGFRTGDIYSAGCKRVGCIEMGEELLKSIESHKLATV 405
           AAVLDTLD+GFRTGDIYSAGCKRVGC EMGEELLKSIESH  A V
Sbjct: 361 AAVLDTLDRGFRTGDIYSAGCKRVGCKEMGEELLKSIESHTPAAV 405

BLAST of MELO3C017853.jh1 vs. NCBI nr
Match: KAG6606821.1 (hypothetical protein SDJN03_00163, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 739 bits (1909), Expect = 6.61e-268
Identity = 375/404 (92.82%), Postives = 387/404 (95.79%), Query Frame = 0

Query: 2   AAALHLNAKPFNLCSPSIRLPKSSPSPFRVKCSAGTSPSRRYNIALLPGDGIGPEVIAVA 61
           AA LHLNAKP N  SPSI L K+SP  FRV CSAGTSPSRR+NIALLPGDG+GPEVIAVA
Sbjct: 9   AATLHLNAKPSNFFSPSISLSKTSPKLFRVTCSAGTSPSRRFNIALLPGDGVGPEVIAVA 68

Query: 62  KDVLNFAASLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKESDAILFGAIGGYKWDD 121
           KDVLN AASLEGIGFDYKEMPVGGAALDLTGVPLPDETL+AAK+SDA+LFGAIGGYKWDD
Sbjct: 69  KDVLNLAASLEGIGFDYKEMPVGGAALDLTGVPLPDETLIAAKQSDAVLFGAIGGYKWDD 128

Query: 122 NPKHLKPETGLLQLRKGLGVFANLRPATVFPQLVDASTLKREVAEGVDLMVVRELTGGIY 181
           NPKHLKPETGLLQLRKGL VFANLRPATVFPQLV+ASTLKREVAEGVDLMVVRELTGGIY
Sbjct: 129 NPKHLKPETGLLQLRKGLEVFANLRPATVFPQLVNASTLKREVAEGVDLMVVRELTGGIY 188

Query: 182 FGQPRGFKTSENGEEIGFNTEVYAAQEIDRVARVAFNNARKRQGRVCSVDKANVLEASIL 241
           FGQPRGFKTSENGEEIGFNTEVYAA EIDRVARVAF NARKRQGRVCSVDKANVLEAS+L
Sbjct: 189 FGQPRGFKTSENGEEIGFNTEVYAAHEIDRVARVAFENARKRQGRVCSVDKANVLEASML 248

Query: 242 WRKRVTAIAAEYPDVELSHMYVDNAAMQLIREPKQFDTILTNNIFGDILSDAASMITGSI 301
           WRKRVTAIAAEYPDVELSHMYVDNAAMQLIR PKQFDTILTNNIFGDILSDAASMITGSI
Sbjct: 249 WRKRVTAIAAEYPDVELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGSI 308

Query: 302 GMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGLGEENAAKRIEA 361
           GMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYG+GEE+AAKRIEA
Sbjct: 309 GMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGIGEESAAKRIEA 368

Query: 362 AVLDTLDKGFRTGDIYSAGCKRVGCIEMGEELLKSIESHKLATV 405
           AVLDTL++GFRTGDIYSAGCK VGC  MGEELLKSIESHKLATV
Sbjct: 369 AVLDTLERGFRTGDIYSAGCKLVGCKAMGEELLKSIESHKLATV 412

BLAST of MELO3C017853.jh1 vs. NCBI nr
Match: XP_023525901.1 (3-isopropylmalate dehydrogenase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 739 bits (1908), Expect = 9.39e-268
Identity = 374/404 (92.57%), Postives = 387/404 (95.79%), Query Frame = 0

Query: 2   AAALHLNAKPFNLCSPSIRLPKSSPSPFRVKCSAGTSPSRRYNIALLPGDGIGPEVIAVA 61
           AA LHLNAKP N  SPSI L K+SP  FRV CSAGTSPSRR+NIALLPGDG+GPEVIAVA
Sbjct: 9   AATLHLNAKPSNFFSPSISLSKTSPKLFRVTCSAGTSPSRRFNIALLPGDGVGPEVIAVA 68

Query: 62  KDVLNFAASLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKESDAILFGAIGGYKWDD 121
           KDVLN AASLEGIGFDYKEMPVGGAALDLTGVPLPDETL+AAK+SDA+LFGAIGGYKWDD
Sbjct: 69  KDVLNLAASLEGIGFDYKEMPVGGAALDLTGVPLPDETLIAAKQSDAVLFGAIGGYKWDD 128

Query: 122 NPKHLKPETGLLQLRKGLGVFANLRPATVFPQLVDASTLKREVAEGVDLMVVRELTGGIY 181
           NPKHLKPETGLLQLRKGL VFANLRPATVFPQLV+ASTLKREVAEGVDLMVVRELTGGIY
Sbjct: 129 NPKHLKPETGLLQLRKGLEVFANLRPATVFPQLVNASTLKREVAEGVDLMVVRELTGGIY 188

Query: 182 FGQPRGFKTSENGEEIGFNTEVYAAQEIDRVARVAFNNARKRQGRVCSVDKANVLEASIL 241
           FGQPRGFKTSENGEEIGFNTEVYAA EIDRVARVAF NARKRQGRVCSVDKANVLEAS+L
Sbjct: 189 FGQPRGFKTSENGEEIGFNTEVYAAHEIDRVARVAFENARKRQGRVCSVDKANVLEASML 248

Query: 242 WRKRVTAIAAEYPDVELSHMYVDNAAMQLIREPKQFDTILTNNIFGDILSDAASMITGSI 301
           WRKRVTAIAAEYPD+ELSHMYVDNAAMQLIR PKQFDTILTNNIFGDILSDAASMITGSI
Sbjct: 249 WRKRVTAIAAEYPDIELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGSI 308

Query: 302 GMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGLGEENAAKRIEA 361
           GMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYG+GEE+AAKRIEA
Sbjct: 309 GMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGIGEESAAKRIEA 368

Query: 362 AVLDTLDKGFRTGDIYSAGCKRVGCIEMGEELLKSIESHKLATV 405
           AVLDTL++GFRTGDIYSAGCK VGC  MGEELLKSIESHKLATV
Sbjct: 369 AVLDTLERGFRTGDIYSAGCKLVGCKAMGEELLKSIESHKLATV 412

BLAST of MELO3C017853.jh1 vs. ExPASy Swiss-Prot
Match: P29102 (3-isopropylmalate dehydrogenase, chloroplastic OS=Brassica napus OX=3708 PE=2 SV=1)

HSP 1 Score: 643.7 bits (1659), Expect = 1.4e-183
Identity = 323/406 (79.56%), Postives = 359/406 (88.42%), Query Frame = 0

Query: 1   MAAALHLNAKPFNLCSPSIRLPK-SSPSPFRVKCSAGTSPSRRYNIALLPGDGIGPEVIA 60
           MAAAL  N +P    +    L K SSP+PFRV+C+A +   +RYNI LLPGDGIGPEVI+
Sbjct: 1   MAAALQTNIRPVKFPATLRALTKQSSPAPFRVRCAAASPGKKRYNITLLPGDGIGPEVIS 60

Query: 61  VAKDVLNFAASLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKESDAILFGAIGGYKW 120
           +AK+VL  A SLEG+ F ++EMPVGGAALDL GVPLP+ET+ AAKESDA+L GAIGGYKW
Sbjct: 61  IAKNVLQQAGSLEGLEFSFQEMPVGGAALDLVGVPLPEETVSAAKESDAVLLGAIGGYKW 120

Query: 121 DDNPKHLKPETGLLQLRKGLGVFANLRPATVFPQLVDASTLKREVAEGVDLMVVRELTGG 180
           D N KHLKPETGLLQLR GL VFANLRPATV PQLVDASTLKREVAEGVDLMVVRELTGG
Sbjct: 121 DKNEKHLKPETGLLQLRAGLKVFANLRPATVLPQLVDASTLKREVAEGVDLMVVRELTGG 180

Query: 181 IYFGQPRGFKTSENGEEIGFNTEVYAAQEIDRVARVAFNNARKRQGRVCSVDKANVLEAS 240
           IYFG PRG KT+ENGEE+G+NTEVYAA EIDR+ARVAF  ARKR+G++CSVDKANVL+AS
Sbjct: 181 IYFGVPRGIKTNENGEEVGYNTEVYAAHEIDRIARVAFETARKRRGKLCSVDKANVLDAS 240

Query: 241 ILWRKRVTAIAAEYPDVELSHMYVDNAAMQLIREPKQFDTILTNNIFGDILSDAASMITG 300
           ILWR+RVTA+AAEYPDVELSHMYVDNAAMQL+R+PKQFDTI+TNNIFGDILSD ASMITG
Sbjct: 241 ILWRRRVTALAAEYPDVELSHMYVDNAAMQLVRDPKQFDTIVTNNIFGDILSDEASMITG 300

Query: 301 SIGMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGLGEENAAKRI 360
           SIGMLPSASL +SGPGLFEPIHGSAPDIAGQDKANPLAT+LSAAMLLKYGLGEE AAKRI
Sbjct: 301 SIGMLPSASLSDSGPGLFEPIHGSAPDIAGQDKANPLATILSAAMLLKYGLGEEKAAKRI 360

Query: 361 EAAVLDTLDKGFRTGDIYSAGCKRVGCIEMGEELLKSIESHKLATV 406
           E AVL  L+KGFRTGDIYSAG K VGC EMGEE+LKS++SH  A+V
Sbjct: 361 EDAVLGALNKGFRTGDIYSAGTKLVGCKEMGEEVLKSVDSHVQASV 406

BLAST of MELO3C017853.jh1 vs. ExPASy Swiss-Prot
Match: P93832 (3-isopropylmalate dehydrogenase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IMDH2 PE=1 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 8.2e-179
Identity = 311/405 (76.79%), Postives = 354/405 (87.41%), Query Frame = 0

Query: 1   MAAALHLNAKPFNLCSPSIRLPKSSPSPFRVKCSAGTSPSRRYNIALLPGDGIGPEVIAV 60
           MAAAL  N +   + +    + K S +PFRV+C+  +   +RY I LLPGDGIGPEV+++
Sbjct: 1   MAAALQTNIRTVKVPATFRAVSKQSLAPFRVRCAVASPGKKRYTITLLPGDGIGPEVVSI 60

Query: 61  AKDVLNFAASLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKESDAILFGAIGGYKWD 120
           AK+VL  A SLEG+ F+++EMP+GGAALDL GVPLP+ET+ AAKESDA+L GAIGGYKWD
Sbjct: 61  AKNVLQQAGSLEGVEFNFREMPIGGAALDLVGVPLPEETISAAKESDAVLLGAIGGYKWD 120

Query: 121 DNPKHLKPETGLLQLRKGLGVFANLRPATVFPQLVDASTLKREVAEGVDLMVVRELTGGI 180
           +N KHL+PE GLLQ+R  L VFANLRPATV PQLVDASTLKREVAEGVDLMVVRELTGGI
Sbjct: 121 NNEKHLRPEKGLLQIRAALKVFANLRPATVLPQLVDASTLKREVAEGVDLMVVRELTGGI 180

Query: 181 YFGQPRGFKTSENGEEIGFNTEVYAAQEIDRVARVAFNNARKRQGRVCSVDKANVLEASI 240
           YFG+PRG KT+ENGEE+GFNTEVYAA EIDR+ARVAF  ARKR+G++CSVDKANVLEASI
Sbjct: 181 YFGEPRGIKTNENGEEVGFNTEVYAAHEIDRIARVAFETARKRRGKLCSVDKANVLEASI 240

Query: 241 LWRKRVTAIAAEYPDVELSHMYVDNAAMQLIREPKQFDTILTNNIFGDILSDAASMITGS 300
           LWRKRVTA+A+EYPDVELSHMYVDNAAMQL+R+PKQFDTI+TNNIFGDILSD ASMITGS
Sbjct: 241 LWRKRVTALASEYPDVELSHMYVDNAAMQLVRDPKQFDTIVTNNIFGDILSDEASMITGS 300

Query: 301 IGMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGLGEENAAKRIE 360
           IGMLPSASL +SGPGLFEPIHGSAPDIAGQDKANPLAT+LSAAMLLKYGLGEE AAKRIE
Sbjct: 301 IGMLPSASLSDSGPGLFEPIHGSAPDIAGQDKANPLATILSAAMLLKYGLGEEKAAKRIE 360

Query: 361 AAVLDTLDKGFRTGDIYSAGCKRVGCIEMGEELLKSIESHKLATV 406
            AVL  L+ GFRTGDIYSAG K VGC EMGEE+LKS++S   A+V
Sbjct: 361 DAVLVALNNGFRTGDIYSAGTKLVGCKEMGEEVLKSVDSQVPASV 405

BLAST of MELO3C017853.jh1 vs. ExPASy Swiss-Prot
Match: Q9FMT1 (3-isopropylmalate dehydrogenase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IMDH1 PE=1 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 7.9e-174
Identity = 302/379 (79.68%), Postives = 338/379 (89.18%), Query Frame = 0

Query: 27  SPFRVKCSAGTSPSRRYNIALLPGDGIGPEVIAVAKDVLNFAASLEGIGFDYKEMPVGGA 86
           +P+R++C+A +   +RYNIALLPGDGIGPEVI+VAK+VL  A SLEG+ FD+KEMPVGGA
Sbjct: 31  APYRIRCAAASPGKKRYNIALLPGDGIGPEVISVAKNVLQKAGSLEGLEFDFKEMPVGGA 90

Query: 87  ALDLTGVPLPDETLLAAKESDAILFGAIGGYKWDDNPKHLKPETGLLQLRKGLGVFANLR 146
           ALDL GVPLP+ET  AAK SDAIL GAIGGYKWD N KHL+PE  L  LR+ L VFANLR
Sbjct: 91  ALDLVGVPLPEETFTAAKLSDAILLGAIGGYKWDKNEKHLRPEMALFYLRRDLKVFANLR 150

Query: 147 PATVFPQLVDASTLKREVAEGVDLMVVRELTGGIYFGQPRGFKTSENGEEIGFNTEVYAA 206
           PATV PQLVDASTLK+EVAEGVD+M+VRELTGGIYFG+PRG   +ENGEE+G +TE+YAA
Sbjct: 151 PATVLPQLVDASTLKKEVAEGVDMMIVRELTGGIYFGEPRGITINENGEEVGVSTEIYAA 210

Query: 207 QEIDRVARVAFNNARKRQGRVCSVDKANVLEASILWRKRVTAIAAEYPDVELSHMYVDNA 266
            EIDR+ARVAF  ARKR+G++CSVDKANVL+ASILWRKRVTA+A+EYPDVELSHMYVDNA
Sbjct: 211 HEIDRIARVAFETARKRRGKLCSVDKANVLDASILWRKRVTALASEYPDVELSHMYVDNA 270

Query: 267 AMQLIREPKQFDTILTNNIFGDILSDAASMITGSIGMLPSASLRESGPGLFEPIHGSAPD 326
           AMQLIR+PKQFDTI+TNNIFGDILSD ASMITGSIGMLPSASL ESGPGLFEPIHGSAPD
Sbjct: 271 AMQLIRDPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPD 330

Query: 327 IAGQDKANPLATVLSAAMLLKYGLGEENAAKRIEAAVLDTLDKGFRTGDIYSAGCKRVGC 386
           IAGQDKANPLAT+LSAAMLLKYGLGEE AAKRIE AV+D L+KGFRTGDIYS G K VGC
Sbjct: 331 IAGQDKANPLATILSAAMLLKYGLGEEKAAKRIEDAVVDALNKGFRTGDIYSPGNKLVGC 390

Query: 387 IEMGEELLKSIESHKLATV 406
            EMGEE+LKS+ES   ATV
Sbjct: 391 KEMGEEVLKSVESKVPATV 409

BLAST of MELO3C017853.jh1 vs. ExPASy Swiss-Prot
Match: Q9SA14 (3-isopropylmalate dehydrogenase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IMDH3 PE=1 SV=2)

HSP 1 Score: 604.7 bits (1558), Expect = 7.4e-172
Identity = 293/373 (78.55%), Postives = 335/373 (89.81%), Query Frame = 0

Query: 27  SPFRVKCSAGTSPSRRYNIALLPGDGIGPEVIAVAKDVLNFAASLEGIGFDYKEMPVGGA 86
           +P+R++C+A +   +RYNI LLPGDGIGPEVI+VAK+VL  A  L+G+ FD++EMP GGA
Sbjct: 28  APYRIRCAAASPVKKRYNITLLPGDGIGPEVISVAKNVLQKAGFLQGLEFDFQEMPFGGA 87

Query: 87  ALDLTGVPLPDETLLAAKESDAILFGAIGGYKWDDNPKHLKPETGLLQLRKGLGVFANLR 146
           ALDL GVPLP+ET  AAK+SDAIL GAIGGYKWD N KHL+PE GLL +R+ L VFANLR
Sbjct: 88  ALDLVGVPLPEETSTAAKQSDAILLGAIGGYKWDKNEKHLRPEMGLLNIRRDLNVFANLR 147

Query: 147 PATVFPQLVDASTLKREVAEGVDLMVVRELTGGIYFGQPRGFKTSENGEEIGFNTEVYAA 206
           PATV PQLVDASTLK+EVA+GVD+M+VRELTGGIYFG+PRG   +ENGEE+GFNTE+YAA
Sbjct: 148 PATVLPQLVDASTLKKEVAQGVDMMIVRELTGGIYFGEPRGITINENGEEVGFNTEIYAA 207

Query: 207 QEIDRVARVAFNNARKRQGRVCSVDKANVLEASILWRKRVTAIAAEYPDVELSHMYVDNA 266
            EIDR+ARVAF  ARKR+G++CSVDKANVL+ASILWRKRVTA+A+EYPDVELSHMYVDNA
Sbjct: 208 HEIDRIARVAFETARKRRGKLCSVDKANVLDASILWRKRVTALASEYPDVELSHMYVDNA 267

Query: 267 AMQLIREPKQFDTILTNNIFGDILSDAASMITGSIGMLPSASLRESGPGLFEPIHGSAPD 326
           AMQL+R+PKQFDTI+TNNIFGDILSD ASMITGSIGMLPSASL ESGPGLFEPIHGSAPD
Sbjct: 268 AMQLVRDPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPD 327

Query: 327 IAGQDKANPLATVLSAAMLLKYGLGEENAAKRIEAAVLDTLDKGFRTGDIYSAGCKRVGC 386
           IAGQDKANPLAT+LSAAMLLKYGLGEE AAK IE AV+D L+KGFRTGDIYS G K VGC
Sbjct: 328 IAGQDKANPLATILSAAMLLKYGLGEEKAAKMIEDAVVDALNKGFRTGDIYSPGNKLVGC 387

Query: 387 IEMGEELLKSIES 400
            EMGEE+LKS++S
Sbjct: 388 KEMGEEVLKSVDS 400

BLAST of MELO3C017853.jh1 vs. ExPASy Swiss-Prot
Match: P73960 (3-isopropylmalate dehydrogenase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=leuB PE=3 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 2.8e-139
Identity = 239/359 (66.57%), Postives = 292/359 (81.34%), Query Frame = 0

Query: 40  SRRYNIALLPGDGIGPEVIAVAKDVLNFAASLEGIGFDYKEMPVGGAALDLTGVPLPDET 99
           S+ YN+ LLPGDGIGPE++AVA  VL   A   G  F+++E  +GGAA+D TG PLP+ T
Sbjct: 2   SQTYNVTLLPGDGIGPEIMAVAVAVLGKVADQFGFAFNFQEALIGGAAIDATGQPLPEAT 61

Query: 100 LLAAKESDAILFGAIGGYKWDDNPKHLKPETGLLQLRKGLGVFANLRPATVFPQLVDAST 159
           L  AK+SDA+L  AIGGY WD+ P+  +PETGLL +R+GLG+FANLRPAT+FPQL+DAS+
Sbjct: 62  LAQAKDSDAVLLAAIGGYAWDNLPRSQRPETGLLAIREGLGLFANLRPATIFPQLIDASS 121

Query: 160 LKREVAEGVDLMVVRELTGGIYFGQPRGFKTSENGEEIGFNTEVYAAQEIDRVARVAFNN 219
           LKREV EGVD+MVVRELTGGIYFG+P+G   +E GE+ G NT  Y   EIDR+A+VAF  
Sbjct: 122 LKREVVEGVDIMVVRELTGGIYFGKPKGIFETETGEKRGVNTMAYTVGEIDRIAKVAFET 181

Query: 220 ARKRQGRVCSVDKANVLEASILWRKRVTAIAAEYPDVELSHMYVDNAAMQLIREPKQFDT 279
           ARKR+G++CSVDKANVL+ S LWR RV AIA +YPDVELSH+YVDNAAMQL+R P+QFDT
Sbjct: 182 ARKRRGQLCSVDKANVLDVSQLWRDRVMAIAVDYPDVELSHLYVDNAAMQLVRSPRQFDT 241

Query: 280 ILTNNIFGDILSDAASMITGSIGMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATV 339
           I+T N+FGDILSD A+M+TGSIGMLPSASL   GPGLFEP+HGSAPDIAGQDKANPLA V
Sbjct: 242 IVTGNLFGDILSDIAAMLTGSIGMLPSASLGSDGPGLFEPVHGSAPDIAGQDKANPLAQV 301

Query: 340 LSAAMLLKYGLGEENAAKRIEAAVLDTLDKGFRTGDIYSAGCKRVGCIEMGEELLKSIE 399
           LSAAM+L+YGL +  AA R+E AV   L++G+RTGDI S G + VGC +MGE+LL  ++
Sbjct: 302 LSAAMMLRYGLDQPQAADRLEDAVKKVLEQGYRTGDILSPGTQLVGCRQMGEQLLSILD 360

BLAST of MELO3C017853.jh1 vs. ExPASy TrEMBL
Match: A0A1S3BZA0 (3-isopropylmalate dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103494678 PE=3 SV=1)

HSP 1 Score: 798 bits (2061), Expect = 1.68e-291
Identity = 405/405 (100.00%), Postives = 405/405 (100.00%), Query Frame = 0

Query: 1   MAAALHLNAKPFNLCSPSIRLPKSSPSPFRVKCSAGTSPSRRYNIALLPGDGIGPEVIAV 60
           MAAALHLNAKPFNLCSPSIRLPKSSPSPFRVKCSAGTSPSRRYNIALLPGDGIGPEVIAV
Sbjct: 1   MAAALHLNAKPFNLCSPSIRLPKSSPSPFRVKCSAGTSPSRRYNIALLPGDGIGPEVIAV 60

Query: 61  AKDVLNFAASLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKESDAILFGAIGGYKWD 120
           AKDVLNFAASLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKESDAILFGAIGGYKWD
Sbjct: 61  AKDVLNFAASLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKESDAILFGAIGGYKWD 120

Query: 121 DNPKHLKPETGLLQLRKGLGVFANLRPATVFPQLVDASTLKREVAEGVDLMVVRELTGGI 180
           DNPKHLKPETGLLQLRKGLGVFANLRPATVFPQLVDASTLKREVAEGVDLMVVRELTGGI
Sbjct: 121 DNPKHLKPETGLLQLRKGLGVFANLRPATVFPQLVDASTLKREVAEGVDLMVVRELTGGI 180

Query: 181 YFGQPRGFKTSENGEEIGFNTEVYAAQEIDRVARVAFNNARKRQGRVCSVDKANVLEASI 240
           YFGQPRGFKTSENGEEIGFNTEVYAAQEIDRVARVAFNNARKRQGRVCSVDKANVLEASI
Sbjct: 181 YFGQPRGFKTSENGEEIGFNTEVYAAQEIDRVARVAFNNARKRQGRVCSVDKANVLEASI 240

Query: 241 LWRKRVTAIAAEYPDVELSHMYVDNAAMQLIREPKQFDTILTNNIFGDILSDAASMITGS 300
           LWRKRVTAIAAEYPDVELSHMYVDNAAMQLIREPKQFDTILTNNIFGDILSDAASMITGS
Sbjct: 241 LWRKRVTAIAAEYPDVELSHMYVDNAAMQLIREPKQFDTILTNNIFGDILSDAASMITGS 300

Query: 301 IGMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGLGEENAAKRIE 360
           IGMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGLGEENAAKRIE
Sbjct: 301 IGMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGLGEENAAKRIE 360

Query: 361 AAVLDTLDKGFRTGDIYSAGCKRVGCIEMGEELLKSIESHKLATV 405
           AAVLDTLDKGFRTGDIYSAGCKRVGCIEMGEELLKSIESHKLATV
Sbjct: 361 AAVLDTLDKGFRTGDIYSAGCKRVGCIEMGEELLKSIESHKLATV 405

BLAST of MELO3C017853.jh1 vs. ExPASy TrEMBL
Match: A0A0A0KTH3 (3-isopropylmalate dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_4G006270 PE=3 SV=1)

HSP 1 Score: 778 bits (2008), Expect = 2.01e-283
Identity = 394/405 (97.28%), Postives = 399/405 (98.52%), Query Frame = 0

Query: 1   MAAALHLNAKPFNLCSPSIRLPKSSPSPFRVKCSAGTSPSRRYNIALLPGDGIGPEVIAV 60
           MAAALHLNAKPF+LCSPSI LPK SPS FRVKCSAGTSPSRRYNIALLPGDGIGPEVIAV
Sbjct: 1   MAAALHLNAKPFSLCSPSISLPKISPSSFRVKCSAGTSPSRRYNIALLPGDGIGPEVIAV 60

Query: 61  AKDVLNFAASLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKESDAILFGAIGGYKWD 120
           AKDVLNFAASLEGIGFDYKEMP+GGAALDLTGVPLPDETLLAAK+SDAILFGAIGGYKWD
Sbjct: 61  AKDVLNFAASLEGIGFDYKEMPMGGAALDLTGVPLPDETLLAAKDSDAILFGAIGGYKWD 120

Query: 121 DNPKHLKPETGLLQLRKGLGVFANLRPATVFPQLVDASTLKREVAEGVDLMVVRELTGGI 180
           DNPKHLKPETGLLQLRKGLGVFANLRPA VFPQLV+ASTLKREVAEGVDLMVVRELTGGI
Sbjct: 121 DNPKHLKPETGLLQLRKGLGVFANLRPAIVFPQLVEASTLKREVAEGVDLMVVRELTGGI 180

Query: 181 YFGQPRGFKTSENGEEIGFNTEVYAAQEIDRVARVAFNNARKRQGRVCSVDKANVLEASI 240
           YFGQPRGFKT ENGEEIGFNTEVYAAQEIDRVARVAFNNARKRQGRVCSVDKANVLEASI
Sbjct: 181 YFGQPRGFKTGENGEEIGFNTEVYAAQEIDRVARVAFNNARKRQGRVCSVDKANVLEASI 240

Query: 241 LWRKRVTAIAAEYPDVELSHMYVDNAAMQLIREPKQFDTILTNNIFGDILSDAASMITGS 300
           LWRKRVTAIAAEYPD+ELSHMYVDNAAMQLIREPKQFDTILTNNIFGDILSDAASMITGS
Sbjct: 241 LWRKRVTAIAAEYPDIELSHMYVDNAAMQLIREPKQFDTILTNNIFGDILSDAASMITGS 300

Query: 301 IGMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGLGEENAAKRIE 360
           IGMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGLGEENAAKRIE
Sbjct: 301 IGMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGLGEENAAKRIE 360

Query: 361 AAVLDTLDKGFRTGDIYSAGCKRVGCIEMGEELLKSIESHKLATV 405
           AAVLDTLDKGFRTGDIYSAGCKRVGC EMGEELLKSIESHKLATV
Sbjct: 361 AAVLDTLDKGFRTGDIYSAGCKRVGCKEMGEELLKSIESHKLATV 405

BLAST of MELO3C017853.jh1 vs. ExPASy TrEMBL
Match: A0A6J1G9F4 (3-isopropylmalate dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111452151 PE=3 SV=1)

HSP 1 Score: 738 bits (1906), Expect = 7.60e-268
Identity = 374/404 (92.57%), Postives = 387/404 (95.79%), Query Frame = 0

Query: 2   AAALHLNAKPFNLCSPSIRLPKSSPSPFRVKCSAGTSPSRRYNIALLPGDGIGPEVIAVA 61
           AA LHLNAKP N  SPSI L K+SP  FRV CSAGTSPSRR+NIALLPGDG+GPEVIAVA
Sbjct: 4   AATLHLNAKPSNFFSPSISLSKTSPKLFRVTCSAGTSPSRRFNIALLPGDGVGPEVIAVA 63

Query: 62  KDVLNFAASLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKESDAILFGAIGGYKWDD 121
           KDVLN AASLEGIGFDYKEMPVGGAALDLTGVPLPDETL+AAK+SDA+LFGAIGGYKWDD
Sbjct: 64  KDVLNLAASLEGIGFDYKEMPVGGAALDLTGVPLPDETLIAAKQSDAVLFGAIGGYKWDD 123

Query: 122 NPKHLKPETGLLQLRKGLGVFANLRPATVFPQLVDASTLKREVAEGVDLMVVRELTGGIY 181
           NPKHLKPETGLLQLRKGL VFANLRPATVFPQLV+ASTLKREVAEGVDLMVVRELTGGIY
Sbjct: 124 NPKHLKPETGLLQLRKGLEVFANLRPATVFPQLVNASTLKREVAEGVDLMVVRELTGGIY 183

Query: 182 FGQPRGFKTSENGEEIGFNTEVYAAQEIDRVARVAFNNARKRQGRVCSVDKANVLEASIL 241
           FGQPRGFKT+ENGEEIGFNTEVYAA EIDRVARVAF NARKRQGRVCSVDKANVLEAS+L
Sbjct: 184 FGQPRGFKTTENGEEIGFNTEVYAAHEIDRVARVAFENARKRQGRVCSVDKANVLEASML 243

Query: 242 WRKRVTAIAAEYPDVELSHMYVDNAAMQLIREPKQFDTILTNNIFGDILSDAASMITGSI 301
           WRKRVTAIAAEYPDVELSHMYVDNAAMQLIR PKQFDTILTNNIFGDILSDAASMITGSI
Sbjct: 244 WRKRVTAIAAEYPDVELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGSI 303

Query: 302 GMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGLGEENAAKRIEA 361
           GMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYG+GEE+AAKRIEA
Sbjct: 304 GMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGIGEESAAKRIEA 363

Query: 362 AVLDTLDKGFRTGDIYSAGCKRVGCIEMGEELLKSIESHKLATV 405
           AVLDTL++GFRTGDIYSAGCK VGC  MGEELLKSIESHKLATV
Sbjct: 364 AVLDTLERGFRTGDIYSAGCKLVGCKAMGEELLKSIESHKLATV 407

BLAST of MELO3C017853.jh1 vs. ExPASy TrEMBL
Match: A0A6J1KE29 (3-isopropylmalate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111493011 PE=3 SV=1)

HSP 1 Score: 737 bits (1903), Expect = 2.53e-267
Identity = 373/404 (92.33%), Postives = 387/404 (95.79%), Query Frame = 0

Query: 2   AAALHLNAKPFNLCSPSIRLPKSSPSPFRVKCSAGTSPSRRYNIALLPGDGIGPEVIAVA 61
           AA LHLNAKP N  SPSI L K+SP  FRV CSAGTSPSRR+NIALLPGDG+GPEVIAVA
Sbjct: 8   AATLHLNAKPSNFFSPSISLSKTSPKLFRVTCSAGTSPSRRFNIALLPGDGVGPEVIAVA 67

Query: 62  KDVLNFAASLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKESDAILFGAIGGYKWDD 121
           KDVLN AASLEGIGFDYKEMPVGGAALDLTGVPLPDETL+AAK+SDA+LFGAIGGYKWDD
Sbjct: 68  KDVLNLAASLEGIGFDYKEMPVGGAALDLTGVPLPDETLMAAKQSDAVLFGAIGGYKWDD 127

Query: 122 NPKHLKPETGLLQLRKGLGVFANLRPATVFPQLVDASTLKREVAEGVDLMVVRELTGGIY 181
           NPKHLKPETGLLQLRKGL VFANLRPATVFPQLV+ASTLKREVAEGVDLMVVRELTGGIY
Sbjct: 128 NPKHLKPETGLLQLRKGLEVFANLRPATVFPQLVNASTLKREVAEGVDLMVVRELTGGIY 187

Query: 182 FGQPRGFKTSENGEEIGFNTEVYAAQEIDRVARVAFNNARKRQGRVCSVDKANVLEASIL 241
           FGQPRGFKTSE+GEEIGFNTEVYAA EIDRVARVAF NARKRQGRVCSVDKANVLEAS+L
Sbjct: 188 FGQPRGFKTSEDGEEIGFNTEVYAAHEIDRVARVAFENARKRQGRVCSVDKANVLEASML 247

Query: 242 WRKRVTAIAAEYPDVELSHMYVDNAAMQLIREPKQFDTILTNNIFGDILSDAASMITGSI 301
           WRKRVTAIAAEYPD+ELSHMYVDNAAMQLIR PKQFDTILTNNIFGDILSDAASMITGSI
Sbjct: 248 WRKRVTAIAAEYPDIELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGSI 307

Query: 302 GMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGLGEENAAKRIEA 361
           GMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYG+GEE+AAKRIEA
Sbjct: 308 GMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGIGEESAAKRIEA 367

Query: 362 AVLDTLDKGFRTGDIYSAGCKRVGCIEMGEELLKSIESHKLATV 405
           AVLDTL++GFRTGDIYSAGCK VGC  MGEELLKSIESHKLATV
Sbjct: 368 AVLDTLERGFRTGDIYSAGCKLVGCKAMGEELLKSIESHKLATV 411

BLAST of MELO3C017853.jh1 vs. ExPASy TrEMBL
Match: A0A6J1DGT0 (3-isopropylmalate dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111020932 PE=3 SV=1)

HSP 1 Score: 731 bits (1887), Expect = 5.75e-265
Identity = 369/404 (91.34%), Postives = 385/404 (95.30%), Query Frame = 0

Query: 2   AAALHLNAKPFNLCSPSIRLPKSSPSPFRVKCSAGTSPSRRYNIALLPGDGIGPEVIAVA 61
           AAALHLNAKP NL S SI + K SP  FRV CSAGTSPSRRY+IALLPGDG+GPEVIAVA
Sbjct: 3   AAALHLNAKPVNLFSRSITVSKPSPKLFRVSCSAGTSPSRRYSIALLPGDGVGPEVIAVA 62

Query: 62  KDVLNFAASLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKESDAILFGAIGGYKWDD 121
           KDVLNFAA+LEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAK+SDA+LFGAIGGYKWDD
Sbjct: 63  KDVLNFAATLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKQSDAVLFGAIGGYKWDD 122

Query: 122 NPKHLKPETGLLQLRKGLGVFANLRPATVFPQLVDASTLKREVAEGVDLMVVRELTGGIY 181
           NPKHLKPETGLLQLRKGL VFANLRPATVFPQLVDASTLK+EVAEGVDLMV+RELTGGIY
Sbjct: 123 NPKHLKPETGLLQLRKGLEVFANLRPATVFPQLVDASTLKKEVAEGVDLMVIRELTGGIY 182

Query: 182 FGQPRGFKTSENGEEIGFNTEVYAAQEIDRVARVAFNNARKRQGRVCSVDKANVLEASIL 241
           FGQPRGFKTSENGEEIGFNTEVYA  EIDRVARVAF NARKRQGRVCSVDKANVLEAS+L
Sbjct: 183 FGQPRGFKTSENGEEIGFNTEVYATHEIDRVARVAFENARKRQGRVCSVDKANVLEASML 242

Query: 242 WRKRVTAIAAEYPDVELSHMYVDNAAMQLIREPKQFDTILTNNIFGDILSDAASMITGSI 301
           WRKRV AIAAEYPD+ELSHMYVDNAAMQLIR PKQFDTILTNNIFGDILSDAASMITGSI
Sbjct: 243 WRKRVIAIAAEYPDIELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGSI 302

Query: 302 GMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGLGEENAAKRIEA 361
           GMLPSASLRESGPGLFEPIHGSAPDIAG+DKANPLATVLSAAMLLKYGLGEENAAKR+E 
Sbjct: 303 GMLPSASLRESGPGLFEPIHGSAPDIAGKDKANPLATVLSAAMLLKYGLGEENAAKRVEV 362

Query: 362 AVLDTLDKGFRTGDIYSAGCKRVGCIEMGEELLKSIESHKLATV 405
           AVLDTLD+GFRTGDIYSAGCK VGC EMG+ELLKSIES++ ATV
Sbjct: 363 AVLDTLDRGFRTGDIYSAGCKLVGCKEMGDELLKSIESYEPATV 406

BLAST of MELO3C017853.jh1 vs. TAIR 10
Match: AT1G80560.1 (isopropylmalate dehydrogenase 2 )

HSP 1 Score: 627.9 bits (1618), Expect = 5.8e-180
Identity = 311/405 (76.79%), Postives = 354/405 (87.41%), Query Frame = 0

Query: 1   MAAALHLNAKPFNLCSPSIRLPKSSPSPFRVKCSAGTSPSRRYNIALLPGDGIGPEVIAV 60
           MAAAL  N +   + +    + K S +PFRV+C+  +   +RY I LLPGDGIGPEV+++
Sbjct: 1   MAAALQTNIRTVKVPATFRAVSKQSLAPFRVRCAVASPGKKRYTITLLPGDGIGPEVVSI 60

Query: 61  AKDVLNFAASLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKESDAILFGAIGGYKWD 120
           AK+VL  A SLEG+ F+++EMP+GGAALDL GVPLP+ET+ AAKESDA+L GAIGGYKWD
Sbjct: 61  AKNVLQQAGSLEGVEFNFREMPIGGAALDLVGVPLPEETISAAKESDAVLLGAIGGYKWD 120

Query: 121 DNPKHLKPETGLLQLRKGLGVFANLRPATVFPQLVDASTLKREVAEGVDLMVVRELTGGI 180
           +N KHL+PE GLLQ+R  L VFANLRPATV PQLVDASTLKREVAEGVDLMVVRELTGGI
Sbjct: 121 NNEKHLRPEKGLLQIRAALKVFANLRPATVLPQLVDASTLKREVAEGVDLMVVRELTGGI 180

Query: 181 YFGQPRGFKTSENGEEIGFNTEVYAAQEIDRVARVAFNNARKRQGRVCSVDKANVLEASI 240
           YFG+PRG KT+ENGEE+GFNTEVYAA EIDR+ARVAF  ARKR+G++CSVDKANVLEASI
Sbjct: 181 YFGEPRGIKTNENGEEVGFNTEVYAAHEIDRIARVAFETARKRRGKLCSVDKANVLEASI 240

Query: 241 LWRKRVTAIAAEYPDVELSHMYVDNAAMQLIREPKQFDTILTNNIFGDILSDAASMITGS 300
           LWRKRVTA+A+EYPDVELSHMYVDNAAMQL+R+PKQFDTI+TNNIFGDILSD ASMITGS
Sbjct: 241 LWRKRVTALASEYPDVELSHMYVDNAAMQLVRDPKQFDTIVTNNIFGDILSDEASMITGS 300

Query: 301 IGMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGLGEENAAKRIE 360
           IGMLPSASL +SGPGLFEPIHGSAPDIAGQDKANPLAT+LSAAMLLKYGLGEE AAKRIE
Sbjct: 301 IGMLPSASLSDSGPGLFEPIHGSAPDIAGQDKANPLATILSAAMLLKYGLGEEKAAKRIE 360

Query: 361 AAVLDTLDKGFRTGDIYSAGCKRVGCIEMGEELLKSIESHKLATV 406
            AVL  L+ GFRTGDIYSAG K VGC EMGEE+LKS++S   A+V
Sbjct: 361 DAVLVALNNGFRTGDIYSAGTKLVGCKEMGEEVLKSVDSQVPASV 405

BLAST of MELO3C017853.jh1 vs. TAIR 10
Match: AT5G14200.1 (isopropylmalate dehydrogenase 1 )

HSP 1 Score: 611.3 bits (1575), Expect = 5.6e-175
Identity = 302/379 (79.68%), Postives = 338/379 (89.18%), Query Frame = 0

Query: 27  SPFRVKCSAGTSPSRRYNIALLPGDGIGPEVIAVAKDVLNFAASLEGIGFDYKEMPVGGA 86
           +P+R++C+A +   +RYNIALLPGDGIGPEVI+VAK+VL  A SLEG+ FD+KEMPVGGA
Sbjct: 31  APYRIRCAAASPGKKRYNIALLPGDGIGPEVISVAKNVLQKAGSLEGLEFDFKEMPVGGA 90

Query: 87  ALDLTGVPLPDETLLAAKESDAILFGAIGGYKWDDNPKHLKPETGLLQLRKGLGVFANLR 146
           ALDL GVPLP+ET  AAK SDAIL GAIGGYKWD N KHL+PE  L  LR+ L VFANLR
Sbjct: 91  ALDLVGVPLPEETFTAAKLSDAILLGAIGGYKWDKNEKHLRPEMALFYLRRDLKVFANLR 150

Query: 147 PATVFPQLVDASTLKREVAEGVDLMVVRELTGGIYFGQPRGFKTSENGEEIGFNTEVYAA 206
           PATV PQLVDASTLK+EVAEGVD+M+VRELTGGIYFG+PRG   +ENGEE+G +TE+YAA
Sbjct: 151 PATVLPQLVDASTLKKEVAEGVDMMIVRELTGGIYFGEPRGITINENGEEVGVSTEIYAA 210

Query: 207 QEIDRVARVAFNNARKRQGRVCSVDKANVLEASILWRKRVTAIAAEYPDVELSHMYVDNA 266
            EIDR+ARVAF  ARKR+G++CSVDKANVL+ASILWRKRVTA+A+EYPDVELSHMYVDNA
Sbjct: 211 HEIDRIARVAFETARKRRGKLCSVDKANVLDASILWRKRVTALASEYPDVELSHMYVDNA 270

Query: 267 AMQLIREPKQFDTILTNNIFGDILSDAASMITGSIGMLPSASLRESGPGLFEPIHGSAPD 326
           AMQLIR+PKQFDTI+TNNIFGDILSD ASMITGSIGMLPSASL ESGPGLFEPIHGSAPD
Sbjct: 271 AMQLIRDPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPD 330

Query: 327 IAGQDKANPLATVLSAAMLLKYGLGEENAAKRIEAAVLDTLDKGFRTGDIYSAGCKRVGC 386
           IAGQDKANPLAT+LSAAMLLKYGLGEE AAKRIE AV+D L+KGFRTGDIYS G K VGC
Sbjct: 331 IAGQDKANPLATILSAAMLLKYGLGEEKAAKRIEDAVVDALNKGFRTGDIYSPGNKLVGC 390

Query: 387 IEMGEELLKSIESHKLATV 406
            EMGEE+LKS+ES   ATV
Sbjct: 391 KEMGEEVLKSVESKVPATV 409

BLAST of MELO3C017853.jh1 vs. TAIR 10
Match: AT1G31180.1 (isopropylmalate dehydrogenase 3 )

HSP 1 Score: 604.7 bits (1558), Expect = 5.3e-173
Identity = 293/373 (78.55%), Postives = 335/373 (89.81%), Query Frame = 0

Query: 27  SPFRVKCSAGTSPSRRYNIALLPGDGIGPEVIAVAKDVLNFAASLEGIGFDYKEMPVGGA 86
           +P+R++C+A +   +RYNI LLPGDGIGPEVI+VAK+VL  A  L+G+ FD++EMP GGA
Sbjct: 28  APYRIRCAAASPVKKRYNITLLPGDGIGPEVISVAKNVLQKAGFLQGLEFDFQEMPFGGA 87

Query: 87  ALDLTGVPLPDETLLAAKESDAILFGAIGGYKWDDNPKHLKPETGLLQLRKGLGVFANLR 146
           ALDL GVPLP+ET  AAK+SDAIL GAIGGYKWD N KHL+PE GLL +R+ L VFANLR
Sbjct: 88  ALDLVGVPLPEETSTAAKQSDAILLGAIGGYKWDKNEKHLRPEMGLLNIRRDLNVFANLR 147

Query: 147 PATVFPQLVDASTLKREVAEGVDLMVVRELTGGIYFGQPRGFKTSENGEEIGFNTEVYAA 206
           PATV PQLVDASTLK+EVA+GVD+M+VRELTGGIYFG+PRG   +ENGEE+GFNTE+YAA
Sbjct: 148 PATVLPQLVDASTLKKEVAQGVDMMIVRELTGGIYFGEPRGITINENGEEVGFNTEIYAA 207

Query: 207 QEIDRVARVAFNNARKRQGRVCSVDKANVLEASILWRKRVTAIAAEYPDVELSHMYVDNA 266
            EIDR+ARVAF  ARKR+G++CSVDKANVL+ASILWRKRVTA+A+EYPDVELSHMYVDNA
Sbjct: 208 HEIDRIARVAFETARKRRGKLCSVDKANVLDASILWRKRVTALASEYPDVELSHMYVDNA 267

Query: 267 AMQLIREPKQFDTILTNNIFGDILSDAASMITGSIGMLPSASLRESGPGLFEPIHGSAPD 326
           AMQL+R+PKQFDTI+TNNIFGDILSD ASMITGSIGMLPSASL ESGPGLFEPIHGSAPD
Sbjct: 268 AMQLVRDPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPD 327

Query: 327 IAGQDKANPLATVLSAAMLLKYGLGEENAAKRIEAAVLDTLDKGFRTGDIYSAGCKRVGC 386
           IAGQDKANPLAT+LSAAMLLKYGLGEE AAK IE AV+D L+KGFRTGDIYS G K VGC
Sbjct: 328 IAGQDKANPLATILSAAMLLKYGLGEEKAAKMIEDAVVDALNKGFRTGDIYSPGNKLVGC 387

Query: 387 IEMGEELLKSIES 400
            EMGEE+LKS++S
Sbjct: 388 KEMGEEVLKSVDS 400

BLAST of MELO3C017853.jh1 vs. TAIR 10
Match: AT5G14200.3 (isopropylmalate dehydrogenase 1 )

HSP 1 Score: 581.6 bits (1498), Expect = 4.8e-166
Identity = 285/354 (80.51%), Postives = 319/354 (90.11%), Query Frame = 0

Query: 27  SPFRVKCSAGTSPSRRYNIALLPGDGIGPEVIAVAKDVLNFAASLEGIGFDYKEMPVGGA 86
           +P+R++C+A +   +RYNIALLPGDGIGPEVI+VAK+VL  A SLEG+ FD+KEMPVGGA
Sbjct: 31  APYRIRCAAASPGKKRYNIALLPGDGIGPEVISVAKNVLQKAGSLEGLEFDFKEMPVGGA 90

Query: 87  ALDLTGVPLPDETLLAAKESDAILFGAIGGYKWDDNPKHLKPETGLLQLRKGLGVFANLR 146
           ALDL GVPLP+ET  AAK SDAIL GAIGGYKWD N KHL+PE  L  LR+ L VFANLR
Sbjct: 91  ALDLVGVPLPEETFTAAKLSDAILLGAIGGYKWDKNEKHLRPEMALFYLRRDLKVFANLR 150

Query: 147 PATVFPQLVDASTLKREVAEGVDLMVVRELTGGIYFGQPRGFKTSENGEEIGFNTEVYAA 206
           PATV PQLVDASTLK+EVAEGVD+M+VRELTGGIYFG+PRG   +ENGEE+G +TE+YAA
Sbjct: 151 PATVLPQLVDASTLKKEVAEGVDMMIVRELTGGIYFGEPRGITINENGEEVGVSTEIYAA 210

Query: 207 QEIDRVARVAFNNARKRQGRVCSVDKANVLEASILWRKRVTAIAAEYPDVELSHMYVDNA 266
            EIDR+ARVAF  ARKR+G++CSVDKANVL+ASILWRKRVTA+A+EYPDVELSHMYVDNA
Sbjct: 211 HEIDRIARVAFETARKRRGKLCSVDKANVLDASILWRKRVTALASEYPDVELSHMYVDNA 270

Query: 267 AMQLIREPKQFDTILTNNIFGDILSDAASMITGSIGMLPSASLRESGPGLFEPIHGSAPD 326
           AMQLIR+PKQFDTI+TNNIFGDILSD ASMITGSIGMLPSASL ESGPGLFEPIHGSAPD
Sbjct: 271 AMQLIRDPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPD 330

Query: 327 IAGQDKANPLATVLSAAMLLKYGLGEENAAKRIEAAVLDTLDKGFRTGDIYSAG 381
           IAGQDKANPLAT+LSAAMLLKYGLGEE AAKRIE AV+D L+KGFRTGDIYS G
Sbjct: 331 IAGQDKANPLATILSAAMLLKYGLGEEKAAKRIEDAVVDALNKGFRTGDIYSPG 384

BLAST of MELO3C017853.jh1 vs. TAIR 10
Match: AT5G14200.2 (isopropylmalate dehydrogenase 1 )

HSP 1 Score: 400.6 bits (1028), Expect = 1.5e-111
Identity = 193/259 (74.52%), Postives = 226/259 (87.26%), Query Frame = 0

Query: 27  SPFRVKCSAGTSPSRRYNIALLPGDGIGPEVIAVAKDVLNFAASLEGIGFDYKEMPVGGA 86
           +P+R++C+A +   +RYNIALLPGDGIGPEVI+VAK+VL  A SLEG+ FD+KEMPVGGA
Sbjct: 31  APYRIRCAAASPGKKRYNIALLPGDGIGPEVISVAKNVLQKAGSLEGLEFDFKEMPVGGA 90

Query: 87  ALDLTGVPLPDETLLAAKESDAILFGAIGGYKWDDNPKHLKPETGLLQLRKGLGVFANLR 146
           ALDL GVPLP+ET  AAK SDAIL GAIGGYKWD N KHL+PE  L  LR+ L VFANLR
Sbjct: 91  ALDLVGVPLPEETFTAAKLSDAILLGAIGGYKWDKNEKHLRPEMALFYLRRDLKVFANLR 150

Query: 147 PATVFPQLVDASTLKREVAEGVDLMVVRELTGGIYFGQPRGFKTSENGEEIGFNTEVYAA 206
           PATV PQLVDASTLK+EVAEGVD+M+VRELTGGIYFG+PRG   +ENGEE+G +TE+YAA
Sbjct: 151 PATVLPQLVDASTLKKEVAEGVDMMIVRELTGGIYFGEPRGITINENGEEVGVSTEIYAA 210

Query: 207 QEIDRVARVAFNNARKRQGRVCSVDKANVLEASILWRKRVTAIAAEYPDVELSHMYVDNA 266
            EIDR+ARVAF  ARKR+G++CSVDKANVL+ASILWRKRVTA+A+EYPDVELSHMYVDNA
Sbjct: 211 HEIDRIARVAFETARKRRGKLCSVDKANVLDASILWRKRVTALASEYPDVELSHMYVDNA 270

Query: 267 AMQLIREPKQFDTILTNNI 286
           AMQLIR+PKQ   +LT ++
Sbjct: 271 AMQLIRDPKQ---VLTQSL 286

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008454199.13.48e-291100.00PREDICTED: 3-isopropylmalate dehydrogenase, chloroplastic-like [Cucumis melo][more]
XP_011652988.14.16e-28397.283-isopropylmalate dehydrogenase, chloroplastic [Cucumis sativus] >KGN52920.1 hyp... [more]
XP_038893808.18.60e-27695.313-isopropylmalate dehydrogenase, chloroplastic-like [Benincasa hispida][more]
KAG6606821.16.61e-26892.82hypothetical protein SDJN03_00163, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023525901.19.39e-26892.573-isopropylmalate dehydrogenase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
P291021.4e-18379.563-isopropylmalate dehydrogenase, chloroplastic OS=Brassica napus OX=3708 PE=2 SV... [more]
P938328.2e-17976.793-isopropylmalate dehydrogenase 2, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Q9FMT17.9e-17479.683-isopropylmalate dehydrogenase 1, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Q9SA147.4e-17278.553-isopropylmalate dehydrogenase 3, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
P739602.8e-13966.573-isopropylmalate dehydrogenase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) ... [more]
Match NameE-valueIdentityDescription
A0A1S3BZA01.68e-291100.003-isopropylmalate dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103494678 PE=3 SV=... [more]
A0A0A0KTH32.01e-28397.283-isopropylmalate dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_4G006270 PE=3 ... [more]
A0A6J1G9F47.60e-26892.573-isopropylmalate dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111452151 PE... [more]
A0A6J1KE292.53e-26792.333-isopropylmalate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111493011 PE=3... [more]
A0A6J1DGT05.75e-26591.343-isopropylmalate dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111020932 P... [more]
Match NameE-valueIdentityDescription
AT1G80560.15.8e-18076.79isopropylmalate dehydrogenase 2 [more]
AT5G14200.15.6e-17579.68isopropylmalate dehydrogenase 1 [more]
AT1G31180.15.3e-17378.55isopropylmalate dehydrogenase 3 [more]
AT5G14200.34.8e-16680.51isopropylmalate dehydrogenase 1 [more]
AT5G14200.21.5e-11174.52isopropylmalate dehydrogenase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Harukei-3) v1.41
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR024084Isopropylmalate dehydrogenase-like domainSMARTSM01329Iso_dh_2coord: 44..393
e-value: 2.3E-194
score: 661.7
IPR024084Isopropylmalate dehydrogenase-like domainPFAMPF00180Iso_dhcoord: 44..392
e-value: 1.8E-136
score: 454.9
IPR004429Isopropylmalate dehydrogenaseTIGRFAMTIGR00169TIGR00169coord: 44..393
e-value: 6.0E-156
score: 516.8
IPR004429Isopropylmalate dehydrogenasePANTHERPTHR429793-ISOPROPYLMALATE DEHYDROGENASEcoord: 1..405
IPR004429Isopropylmalate dehydrogenaseHAMAPMF_01033LeuB_type1coord: 43..397
score: 50.539864
NoneNo IPR availableGENE3D3.40.718.10Isopropylmalate Dehydrogenasecoord: 41..404
e-value: 3.7E-134
score: 449.3
NoneNo IPR availablePANTHERPTHR42979:SF73-ISOPROPYLMALATE DEHYDROGENASEcoord: 1..405
NoneNo IPR availableSUPERFAMILY53659Isocitrate/Isopropylmalate dehydrogenase-likecoord: 42..397
IPR019818Isocitrate/isopropylmalate dehydrogenase, conserved sitePROSITEPS00470IDH_IMDHcoord: 284..303

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C017853.jh1.t1MELO3C017853.jh1.t1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009098 leucine biosynthetic process
molecular_function GO:0003862 3-isopropylmalate dehydrogenase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0051287 NAD binding
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor