MELO3C005359.jh1 (gene) Melon (Harukei-3) v1.41

Overview
NameMELO3C005359.jh1
Typegene
OrganismCucumis melo var. reticulatus cv. Harukei-3 (Melon (Harukei-3) v1.41)
DescriptionEukaryotic aspartyl protease family protein
Locationchr09: 20491083 .. 20492462 (-)
RNA-Seq ExpressionMELO3C005359.jh1
SyntenyMELO3C005359.jh1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidestart_codonstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
GCTCTCCATTTCCCCATTCTTCCTTCTCATTCCTCTCTTCTTCTTCTTTCTCACACATCTCTCCAACCCCAATGCCACCGCCGTCGCCGCCGCCGCCGACTTCCTGAAGCTCCCCCTTCTTCACAAACCCCCCTTCTCCTCCCCTTCCCAATCCCTCTCCTCCGACACCCACCGCCTCTCCCTCCTCTTCTCTCGCCCCAACCCCACTCTTAAATCCCCTCTCATCTCCGGCGCCTCCACCGGCTCCGGCCAATACTTCGTCGACATCCGCCTCGGTACCCCTCCCCAAAGCCTCCTTCTTGTCGCCGATACCGGCAGTGACCTCGTCTGGGTCAAATGCTCCGCCTGCCGCAACTGCTCTCACCACCCTCCTTCCTCCGCCTTCTTCCCCCGCCATTCCTCCTCCTTCTCCCCTTTCCACTGCTTCGACCCCCACTGCCGTCTCCTCCCCCATGCTCCTCCCCATCACTGTAACCACACGCTCCTCCACTCCCCTTGCCGCTTCCTCTACTCCTACGCCGATGGCTCCCTCTCCTCCGGCTTCTTCTCCAAAGAAACCACCACATTGAAGACGCTCTCCGGGTCCGAAATCCATCTTAAAGGCCTCTCGTTTGGCTGCGGATTTCGGATCTCCGGTCCCAGCGTTTCGGGGGCTCAGTTCAATGGTGCACGTGGCGTCATGGGATTGGGTAGAGGCTCCATTTCCTTCTCTTCTCAACTCGGCCGCCGATTCGGCAACAAATTTTCTTACTGTCTTATGGATTACACTCTCTCTCCGCCACCCACCAGCTTCTTAATGATCGGCGGCGGCCTCCACAGCCTCCCTGTCAACAATGCCACAAAAATCAGCTACACCCCTTTGCAGATTAACCCTCTTTCCCCCACATTCTACTACATTACCATCAACAGCATCACCATCGACGGCGTGAAATTACCCATCAACCCCGCCGTTTGGGAAATCGACGAACAGGGAAACGGCGGCACGGTGGTGGATTCAGGGACAACACTAACCTACCTAACGAAGACAGCGTACGAGGAGGTGCTGAAGTCAGTAAGACGGCGAGTGAAACTACCAAATGCGGCCGAGTTGACACCGGGGTTCGATCTATGCGTAAACGCGTCGGGAGAGTCGCGGCGGCCGAGTCTGCCACGACTGAGATTCCGACTGGGAGGTGGGGCGGTGTTTGCTCCGCCGCCGAGGAACTATTTTCTGGAAACGGAGGAGGGAGTGATGTGTTTGGCGATCCGAGCGGTGGAATCGGGGAATGGGTTTTCGGTGATTGGAAATCTGATGCAGCAAGGATTCTTGTTGGAGTTCGATAAGGAGGAATCGAGGCTGGGTTTTACAAGGCGGGGCTGTGGGCTTCCATGACGAACGTCATCAACTTTGGAAGTTTGTTGCTTTATTTTTGTTAACTCGACTCACTGAGTTTGGAACTCGCAATCAATTAATTTTCGATTTGGTTAAATTATTATTATTATCATCATCATCATTATTATTATTAAAAAAGAGGTTTTTAAATTTTTAATAATAATAAGTTTGTACTTTGGATTGGATTCTCTTTTGTATACTACAAAAAGTCATTGATTTTTGGACATTGTGTTTTATTTGTCTACTTCTGATTTGTATCACACCATCCTTTTCCCCCCAACTTCACTTATATTTATATAATCATAATTTTATTTCTA

mRNA sequence

GCTCTCCATTTCCCCATTCTTCCTTCTCATTCCTCTCTTCTTCTTCTTTCTCACACATCTCTCCAACCCCAATGCCACCGCCGTCGCCGCCGCCGCCGACTTCCTGAAGCTCCCCCTTCTTCACAAACCCCCCTTCTCCTCCCCTTCCCAATCCCTCTCCTCCGACACCCACCGCCTCTCCCTCCTCTTCTCTCGCCCCAACCCCACTCTTAAATCCCCTCTCATCTCCGGCGCCTCCACCGGCTCCGGCCAATACTTCGTCGACATCCGCCTCGGTACCCCTCCCCAAAGCCTCCTTCTTGTCGCCGATACCGGCAGTGACCTCGTCTGGGTCAAATGCTCCGCCTGCCGCAACTGCTCTCACCACCCTCCTTCCTCCGCCTTCTTCCCCCGCCATTCCTCCTCCTTCTCCCCTTTCCACTGCTTCGACCCCCACTGCCGTCTCCTCCCCCATGCTCCTCCCCATCACTGTAACCACACGCTCCTCCACTCCCCTTGCCGCTTCCTCTACTCCTACGCCGATGGCTCCCTCTCCTCCGGCTTCTTCTCCAAAGAAACCACCACATTGAAGACGCTCTCCGGGTCCGAAATCCATCTTAAAGGCCTCTCGTTTGGCTGCGGATTTCGGATCTCCGGTCCCAGCGTTTCGGGGGCTCAGTTCAATGGTGCACGTGGCGTCATGGGATTGGGTAGAGGCTCCATTTCCTTCTCTTCTCAACTCGGCCGCCGATTCGGCAACAAATTTTCTTACTGTCTTATGGATTACACTCTCTCTCCGCCACCCACCAGCTTCTTAATGATCGGCGGCGGCCTCCACAGCCTCCCTGTCAACAATGCCACAAAAATCAGCTACACCCCTTTGCAGATTAACCCTCTTTCCCCCACATTCTACTACATTACCATCAACAGCATCACCATCGACGGCGTGAAATTACCCATCAACCCCGCCGTTTGGGAAATCGACGAACAGGGAAACGGCGGCACGGTGGTGGATTCAGGGACAACACTAACCTACCTAACGAAGACAGCGTACGAGGAGGTGCTGAAGTCAGTAAGACGGCGAGTGAAACTACCAAATGCGGCCGAGTTGACACCGGGGTTCGATCTATGCGTAAACGCGTCGGGAGAGTCGCGGCGGCCGAGTCTGCCACGACTGAGATTCCGACTGGGAGGTGGGGCGGTGTTTGCTCCGCCGCCGAGGAACTATTTTCTGGAAACGGAGGAGGGAGTGATGTGTTTGGCGATCCGAGCGGTGGAATCGGGGAATGGGTTTTCGGTGATTGGAAATCTGATGCAGCAAGGATTCTTGTTGGAGTTCGATAAGGAGGAATCGAGGCTGGGTTTTACAAGGCGGGGCTGTGGGCTTCCATGACGAACGTCATCAACTTTGGAAGTTTGTTGCTTTATTTTTGTTAACTCGACTCACTGAGTTTGGAACTCGCAATCAATTAATTTTCGATTTGGTTAAATTATTATTATTATCATCATCATCATTATTATTATTAAAAAAGAGGTTTTTAAATTTTTAATAATAATAAGTTTGTACTTTGGATTGGATTCTCTTTTGTATACTACAAAAAGTCATTGATTTTTGGACATTGTGTTTTATTTGTCTACTTCTGATTTGTATCACACCATCCTTTTCCCCCCAACTTCACTTATATTTATATAATCATAATTTTATTTCTA

Coding sequence (CDS)

ATGGGATTGGGTAGAGGCTCCATTTCCTTCTCTTCTCAACTCGGCCGCCGATTCGGCAACAAATTTTCTTACTGTCTTATGGATTACACTCTCTCTCCGCCACCCACCAGCTTCTTAATGATCGGCGGCGGCCTCCACAGCCTCCCTGTCAACAATGCCACAAAAATCAGCTACACCCCTTTGCAGATTAACCCTCTTTCCCCCACATTCTACTACATTACCATCAACAGCATCACCATCGACGGCGTGAAATTACCCATCAACCCCGCCGTTTGGGAAATCGACGAACAGGGAAACGGCGGCACGGTGGTGGATTCAGGGACAACACTAACCTACCTAACGAAGACAGCGTACGAGGAGGTGCTGAAGTCAGTAAGACGGCGAGTGAAACTACCAAATGCGGCCGAGTTGACACCGGGGTTCGATCTATGCGTAAACGCGTCGGGAGAGTCGCGGCGGCCGAGTCTGCCACGACTGAGATTCCGACTGGGAGGTGGGGCGGTGTTTGCTCCGCCGCCGAGGAACTATTTTCTGGAAACGGAGGAGGGAGTGATGTGTTTGGCGATCCGAGCGGTGGAATCGGGGAATGGGTTTTCGGTGATTGGAAATCTGATGCAGCAAGGATTCTTGTTGGAGTTCGATAAGGAGGAATCGAGGCTGGGTTTTACAAGGCGGGGCTGTGGGCTTCCATGA

Protein sequence

MGLGRGSISFSSQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPVNNATKISYTPLQINPLSPTFYYITINSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLPNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP
Homology
BLAST of MELO3C005359.jh1 vs. NCBI nr
Match: XP_008467208.1 (PREDICTED: aspartyl protease family protein 2 [Cucumis melo])

HSP 1 Score: 932 bits (2409), Expect = 0.0
Identity = 459/459 (100.00%), Postives = 459/459 (100.00%), Query Frame = 0

Query: 1   MPMLSISPFFLLIPLFFFFLTHLSNPNATAVAAAADFLKLPLLHKPPFSSPSQSLSSDTH 60
           MPMLSISPFFLLIPLFFFFLTHLSNPNATAVAAAADFLKLPLLHKPPFSSPSQSLSSDTH
Sbjct: 1   MPMLSISPFFLLIPLFFFFLTHLSNPNATAVAAAADFLKLPLLHKPPFSSPSQSLSSDTH 60

Query: 61  RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR 120
           RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR
Sbjct: 61  RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR 120

Query: 121 NCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHAPPHHCNHTLLHSPCRFLYSYADGSL 180
           NCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHAPPHHCNHTLLHSPCRFLYSYADGSL
Sbjct: 121 NCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHAPPHHCNHTLLHSPCRFLYSYADGSL 180

Query: 181 SSGFFSKETTTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS 240
           SSGFFSKETTTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS
Sbjct: 181 SSGFFSKETTTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS 240

Query: 241 SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPVNNATKISYTPLQINPLSPTFY 300
           SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPVNNATKISYTPLQINPLSPTFY
Sbjct: 241 SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPVNNATKISYTPLQINPLSPTFY 300

Query: 301 YITINSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL 360
           YITINSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL
Sbjct: 301 YITINSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL 360

Query: 361 PNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA 420
           PNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA
Sbjct: 361 PNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA 420

Query: 421 VESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459
           VESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP
Sbjct: 421 VESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459

BLAST of MELO3C005359.jh1 vs. NCBI nr
Match: XP_004143702.1 (aspartyl protease family protein 2 [Cucumis sativus] >KGN50439.1 hypothetical protein Csa_000435 [Cucumis sativus])

HSP 1 Score: 900 bits (2327), Expect = 0.0
Identity = 446/459 (97.17%), Postives = 450/459 (98.04%), Query Frame = 0

Query: 1   MPMLSISPFFLLIPLFFFFLTHLSNPNATAVAAAADFLKLPLLHKPPFSSPSQSLSSDTH 60
           MP+LSISPFFLLI LF FFLTHL NPNATAVAA ADFLKLPLLHKPPFSSPSQSLSSDTH
Sbjct: 1   MPVLSISPFFLLILLFSFFLTHLPNPNATAVAAPADFLKLPLLHKPPFSSPSQSLSSDTH 60

Query: 61  RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR 120
           RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR
Sbjct: 61  RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR 120

Query: 121 NCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHAPPHHCNHTLLHSPCRFLYSYADGSL 180
           NCSHHPPSSAF PRHSSSFSPFHCFDPHCRLLPHAP H CNHT LHSPCRFLYSYADGSL
Sbjct: 121 NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSL 180

Query: 181 SSGFFSKETTTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS 240
           SSGFFSKETTTLK+LSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS
Sbjct: 181 SSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS 240

Query: 241 SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPVNNATKISYTPLQINPLSPTFY 300
           SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLP+ NATKISYTPLQINPLSPTFY
Sbjct: 241 SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFY 300

Query: 301 YITINSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL 360
           YITI+SITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL
Sbjct: 301 YITIHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL 360

Query: 361 PNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA 420
           PNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA
Sbjct: 361 PNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA 420

Query: 421 VESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459
           VESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP
Sbjct: 421 VESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459

BLAST of MELO3C005359.jh1 vs. NCBI nr
Match: XP_038907006.1 (aspartyl protease family protein 2 [Benincasa hispida])

HSP 1 Score: 825 bits (2130), Expect = 4.91e-300
Identity = 409/457 (89.50%), Postives = 428/457 (93.65%), Query Frame = 0

Query: 3   MLSISPFFLLIPLFFFFLTHLSNPNATAVAAAADFLKLPLLHKPPFSSPSQSLSSDTHRL 62
           ML ISPFF  I LFFF    L+N NATA    AD+LKLPLLHKPPFSSPSQ+LSSDTHRL
Sbjct: 1   MLPISPFFHFIFLFFF----LTNFNATA----ADYLKLPLLHKPPFSSPSQALSSDTHRL 60

Query: 63  SLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNC 122
           SLLFSRPNPTLKSPLISGASTGSGQYFVD+RLGTPPQSLLLVADTGSDLVWVKCSACRNC
Sbjct: 61  SLLFSRPNPTLKSPLISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSACRNC 120

Query: 123 SHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHAPPHHCNHTLLHSPCRFLYSYADGSLSS 182
           SHHPPS+AF PRHSSSFSPFHCFDPHCRLLPHAPPH CNHT  HSPCRFLYSYADGSLSS
Sbjct: 121 SHHPPSTAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRFHSPCRFLYSYADGSLSS 180

Query: 183 GFFSKETTTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQ 242
           GFFSKETTTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQF+GARGVMGLGRGSISFSSQ
Sbjct: 181 GFFSKETTTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQFSGARGVMGLGRGSISFSSQ 240

Query: 243 LGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPVNNATKISYTPLQINPLSPTFYYI 302
           LGRRFGNKFSYCLMDYTLSPPPTS+LMIGGG  SLPV NATKISYTPLQINPLSPTFYYI
Sbjct: 241 LGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGHRSLPVTNATKISYTPLQINPLSPTFYYI 300

Query: 303 TINSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLPN 362
            I+SIT+DGVKLPINPAVW +D+QGNGGTVVDSGTTLTYL K AY+EVLK+VRRRVKLP+
Sbjct: 301 AIHSITVDGVKLPINPAVWAMDKQGNGGTVVDSGTTLTYLAKAAYDEVLKAVRRRVKLPS 360

Query: 363 AAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVE 422
           A+ELTPGFDLCVNAS  SRRPSLPRLRFRLGGGAVFAPPPRNYFLETEE VMCLAIR V+
Sbjct: 361 ASELTPGFDLCVNASDSSRRPSLPRLRFRLGGGAVFAPPPRNYFLETEERVMCLAIRPVD 420

Query: 423 SGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459
           SGNGFSVIGNLMQQGFLLEFDK+ +RLGF+RRGCGLP
Sbjct: 421 SGNGFSVIGNLMQQGFLLEFDKDAARLGFSRRGCGLP 449

BLAST of MELO3C005359.jh1 vs. NCBI nr
Match: XP_023549997.1 (aspartyl protease family protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 795 bits (2054), Expect = 2.71e-288
Identity = 398/461 (86.33%), Postives = 417/461 (90.46%), Query Frame = 0

Query: 3   MLSISPFFLLIPLFFFFLTHLSNPNATAVAAAADFLKLPLLHKPPFSSPSQSLSSDTHRL 62
           MLS+SPFF  I L  F L  L N      AAAAD+LKLPLLHK PFSSPSQ+LSSDTHRL
Sbjct: 5   MLSVSPFFHFI-LLLFSLADLLN-----AAAAADYLKLPLLHKNPFSSPSQALSSDTHRL 64

Query: 63  SLLFS----RPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSA 122
           SLLFS    RPNPTLKSPLISGASTGSGQYFVD+R+GTPPQSLLLVADTGSDLVWVKCSA
Sbjct: 65  SLLFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSA 124

Query: 123 CRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHAPPHHCNHTLLHSPCRFLYSYADG 182
           CRNCSHHPPSSAF PRHSSSFSPFHCFDPHCRLLPHAPPH CNHT LHSPCRFLY+YADG
Sbjct: 125 CRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADG 184

Query: 183 SLSSGFFSKETTTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSIS 242
           S SSGFFSKETTTLKTLSGSE  LK LSFGCGFRISGPSVSGAQFNGARGVMGLGRG IS
Sbjct: 185 STSSGFFSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPIS 244

Query: 243 FSSQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPVNNATKISYTPLQINPLSPT 302
           FS+QLGRRFGNKFSYCLMDYTLSPPPTS+LMIGGGL SLPV NATKISYTPL INPLSPT
Sbjct: 245 FSTQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPT 304

Query: 303 FYYITINSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRV 362
           FYYI + SIT+DGVKLPINP VW IDEQGNGGTVVDSGTTLTYL + AY+EVLK+VR+RV
Sbjct: 305 FYYIAVKSITVDGVKLPINPTVWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAVRQRV 364

Query: 363 KLPNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAI 422
           KLP AAELTPGFDLCVN S ES+RPSLPR+RF+LG GAVFAPP RNYFLETEEGVMCL+I
Sbjct: 365 KLPAAAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFLETEEGVMCLSI 424

Query: 423 RAVESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459
           RAVE GNGFSVIGNLMQQGFLLEFDKE SRLGF+RRGCGLP
Sbjct: 425 RAVEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

BLAST of MELO3C005359.jh1 vs. NCBI nr
Match: KAG7017139.1 (Aspartic proteinase nepenthesin-2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 792 bits (2045), Expect = 5.47e-287
Identity = 396/461 (85.90%), Postives = 417/461 (90.46%), Query Frame = 0

Query: 3   MLSISPFFLLIPLFFFFLTHLSNPNATAVAAAADFLKLPLLHKPPFSSPSQSLSSDTHRL 62
           MLS+SPFF  I L  F L  L N      AAAAD+LKLPLLHK PFSSPSQ+LSSDTHRL
Sbjct: 1   MLSVSPFFHFI-LLLFSLADLLN-----AAAAADYLKLPLLHKNPFSSPSQALSSDTHRL 60

Query: 63  SLLFS----RPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSA 122
           SLLFS    RPNPTLKSPLISGASTGSGQYFVD+R+GTPPQSLLLVADTGSDLVWVKCSA
Sbjct: 61  SLLFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSA 120

Query: 123 CRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHAPPHHCNHTLLHSPCRFLYSYADG 182
           CRNCSHHPPSSAF PRHSSSFSPFHCFDPHCRLLPHAPPH CNHT LHSPCRFLY+YADG
Sbjct: 121 CRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADG 180

Query: 183 SLSSGFFSKETTTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSIS 242
           S SSGFFSKETTTLKTLSGSE  LK LSFGCGFRISGPSVSGAQFNGARGVMGLGRG IS
Sbjct: 181 STSSGFFSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPIS 240

Query: 243 FSSQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPVNNATKISYTPLQINPLSPT 302
           FS+QLGRRFGNKFSYCLMDYTLSPPPTS+LMIGGGL SLPV NATKISYTPL INPLSPT
Sbjct: 241 FSTQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPT 300

Query: 303 FYYITINSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRV 362
           FYYI + SIT+DGVKLPINP VW I+EQGNGGTVVDSGTTLTYL + AY+EVLK++R+RV
Sbjct: 301 FYYIAVKSITVDGVKLPINPIVWAINEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRV 360

Query: 363 KLPNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAI 422
           KLP AAELTPGFDLCVN S ES+RPSLPR+RF+LG GAVFAPP RNYFL+TEEGVMCLAI
Sbjct: 361 KLPAAAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFLDTEEGVMCLAI 420

Query: 423 RAVESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459
           RAVE GNGFSVIGNLMQQGFLLEFDKE SRLGF+RRGCGLP
Sbjct: 421 RAVEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 455

BLAST of MELO3C005359.jh1 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 2.1e-58
Identity = 140/386 (36.27%), Postives = 206/386 (53.37%), Query Frame = 0

Query: 75  SPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFFPR 134
           S ++SG S GSG+YF  + +GTP + + +V DTGSD+VW++C+ CR C +      F PR
Sbjct: 129 SSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRC-YSQSDPIFDPR 188

Query: 135 HSSSFSPFHCFDPHCRLLPHAPPHHCNHTLLHSPCRFLYSYADGSLSSGFFSKETTTLKT 194
            S +++   C  PHCR L  A    CN       C +  SY DGS + G FS ET T + 
Sbjct: 189 KSKTYATIPCSSPHCRRLDSA---GCN--TRRKTCLYQVSYGDGSFTVGDFSTETLTFR- 248

Query: 195 LSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQLGRRFGNKFSYC 254
                  +KG++ GCG    G       F GA G++GLG+G +SF  Q G RF  KFSYC
Sbjct: 249 ----RNRVKGVALGCGHDNEG------LFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYC 308

Query: 255 LMDYTLSPPPTSFLMIGGGLHSLPVNNATKISYTPLQINPLSPTFYYITINSITIDGVKL 314
           L+D + S  P+S +     +  +         +TPL  NP   TFYY+ +  I++ G ++
Sbjct: 309 LVDRSASSKPSSVVFGNAAVSRI-------ARFTPLLSNPKLDTFYYVGLLGISVGGTRV 368

Query: 315 P-INPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLPNAAELTPGFDLC 374
           P +  +++++D+ GNGG ++DSGT++T L + AY  +  + R   K    A     FD C
Sbjct: 369 PGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTC 428

Query: 375 VNAS--GESRRPSLPRLRFRLGGGAVFAPPPRNYFLETE-EGVMCLAIRAVESGNGFSVI 434
            + S   E + P++  L FR   GA  + P  NY +  +  G  C A     +  G S+I
Sbjct: 429 FDLSNMNEVKVPTVV-LHFR---GADVSLPATNYLIPVDTNGKFCFAFAG--TMGGLSII 484

Query: 435 GNLMQQGFLLEFDKEESRLGFTRRGC 457
           GN+ QQGF + +D   SR+GF   GC
Sbjct: 489 GNIQQQGFRVVYDLASSRVGFAPGGC 484

BLAST of MELO3C005359.jh1 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 9.3e-54
Identity = 127/374 (33.96%), Postives = 194/374 (51.87%), Query Frame = 0

Query: 84  GSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFFPRHSSSFSPFH 143
           G G+Y +++ +GTP  S   + DTGSDL+W +C  C  C    P+  F P+ SSSFS   
Sbjct: 92  GDGEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQC-FSQPTPIFNPQDSSSFSTLP 151

Query: 144 CFDPHCRLLPHAPPHHCNHTLLHSPCRFLYSYADGSLSSGFFSKETTTLKTLSGSEIHLK 203
           C   +C+ LP       + T  ++ C++ Y Y DGS + G+ + ET T +T S     + 
Sbjct: 152 CESQYCQDLP-------SETCNNNECQYTYGYGDGSTTQGYMATETFTFETSS-----VP 211

Query: 204 GLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQLGRRFGNKFSYCLMDYTLSPP 263
            ++FGCG    G      Q NGA G++G+G G +S  SQLG     +FSYC+  Y  S P
Sbjct: 212 NIAFGCGEDNQG----FGQGNGA-GLIGMGWGPLSLPSQLG---VGQFSYCMTSYGSSSP 271

Query: 264 PTSFLMIGGGLHSLPVNNATKISYTPLQINPLSPTFYYITINSITIDGVKLPINPAVWEI 323
            T  L +G     +P  + +    T L  + L+PT+YYIT+  IT+ G  L I  + +++
Sbjct: 272 ST--LALGSAASGVPEGSPS----TTLIHSSLNPTYYYITLQGITVGGDNLGIPSSTFQL 331

Query: 324 DEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLPNAAELTPGFDLCVNASGESRRP 383
            + G GG ++DSGTTLTYL + AY  V ++   ++ LP   E + G   C     +    
Sbjct: 332 QDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQINLPTVDESSSGLSTCFQQPSDGSTV 391

Query: 384 SLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVESGNGFSVIGNLMQQGFLLEFD 443
            +P +  +  GG V     +N  +   EGV+CLA+ +  S  G S+ GN+ QQ   + +D
Sbjct: 392 QVPEISMQFDGG-VLNLGEQNILISPAEGVICLAMGS-SSQLGISIFGNIQQQETQVLYD 436

Query: 444 KEESRLGFTRRGCG 458
            +   + F    CG
Sbjct: 452 LQNLAVSFVPTQCG 436

BLAST of MELO3C005359.jh1 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 3.3e-51
Identity = 146/464 (31.47%), Postives = 221/464 (47.63%), Query Frame = 0

Query: 5   SISPFFLLIPLFFFFLTHLSNPNATAV----AAAADFLKLPLLHKPPFSSPS--QSLSSD 64
           S+  F L + + + F+    + + TA+     A     ++ L H     + +  Q L   
Sbjct: 4   SLYSFLLALSIVYIFVAPTHSTSRTALNHRHEAKVTGFQIMLEHVDSGKNLTKFQLLERA 63

Query: 65  THRLSLLFSRPNPTLKSPLISGAST----GSGQYFVDIRLGTPPQSLLLVADTGSDLVWV 124
             R S    R    L  P  SG  T    G G+Y +++ +GTP Q    + DTGSDL+W 
Sbjct: 64  IERGSRRLQRLEAMLNGP--SGVETSVYAGDGEYLMNLSIGTPAQPFSAIMDTGSDLIWT 123

Query: 125 KCSACRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHAPPHHCNHTLLHSPCRFLYS 184
           +C  C  C  +  +  F P+ SSSFS   C    C+ L        + T  ++ C++ Y 
Sbjct: 124 QCQPCTQC-FNQSTPIFNPQGSSSFSTLPCSSQLCQAL-------SSPTCSNNFCQYTYG 183

Query: 185 YADGSLSSGFFSKETTTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGR 244
           Y DGS + G    ET T  ++S     +  ++FGCG    G      Q NGA G++G+GR
Sbjct: 184 YGDGSETQGSMGTETLTFGSVS-----IPNITFGCGENNQG----FGQGNGA-GLVGMGR 243

Query: 245 GSISFSSQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPVNNATKISYTPLQINP 304
           G +S  SQL      KFSYC+     S P  S L++G   +S+   +         QI  
Sbjct: 244 GPLSLPSQLD---VTKFSYCMTPIGSSTP--SNLLLGSLANSVTAGSPNTTLIQSSQI-- 303

Query: 305 LSPTFYYITINSITIDGVKLPINPAVWEID-EQGNGGTVVDSGTTLTYLTKTAYEEVLKS 364
             PTFYYIT+N +++   +LPI+P+ + ++   G GG ++DSGTTLTY    AY+ V + 
Sbjct: 304 --PTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQE 363

Query: 365 VRRRVKLPNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGV 424
              ++ LP     + GFDLC     +     +P       GG +   P  NYF+    G+
Sbjct: 364 FISQINLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGGDL-ELPSENYFISPSNGL 423

Query: 425 MCLAIRAVESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCG 458
           +CLA+ +  S  G S+ GN+ QQ  L+ +D   S + F    CG
Sbjct: 424 ICLAMGS--SSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQCG 435

BLAST of MELO3C005359.jh1 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 3.6e-50
Identity = 155/501 (30.94%), Postives = 236/501 (47.11%), Query Frame = 0

Query: 11  LLIPLFFFFL---THLSN----------------PNATAVAAAADF------------LK 70
           +L+PLFFFFL    HLS+                P  T  A   DF              
Sbjct: 1   MLLPLFFFFLHLHLHLSSSSSISFPDFQIIDVLQPPLTVTATLPDFNNTHFSDESSSKYT 60

Query: 71  LPLLHKPPFSSPS---------QSLSSDTHRLSLLFSR------PNPTLK-------SPL 130
           L LLH+  F S +           +  DT R+S +  R      P+   +       S +
Sbjct: 61  LRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPSSDSRYEVNDFGSDI 120

Query: 131 ISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFFPRHSS 190
           +SG   GSG+YFV I +G+PP+   +V D+GSD+VWV+C  C+ C +      F P  S 
Sbjct: 121 VSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLC-YKQSDPVFDPAKSG 180

Query: 191 SFSPFHCFDPHCRLLPHAPPHHCNHTLLHSPCRFLYSYADGSLSSGFFSKETTTLKTLSG 250
           S++   C    C  + ++  H          CR+   Y DGS     ++K T  L+TL+ 
Sbjct: 181 SYTGVSCGSSVCDRIENSGCH-------SGGCRYEVMYGDGS-----YTKGTLALETLTF 240

Query: 251 SEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQLGRRFGNKFSYCLMD 310
           ++  ++ ++ GCG R  G       F GA G++G+G GS+SF  QL  + G  F YCL+ 
Sbjct: 241 AKTVVRNVAMGCGHRNRG------MFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLV- 300

Query: 311 YTLSPPPTSFLMIGGGLHSLPVNNATKISYTPLQINPLSPTFYYITINSITIDGVKLPIN 370
            +     T  L+   G  +LPV      S+ PL  NP +P+FYY+ +  + + GV++P+ 
Sbjct: 301 -SRGTDSTGSLVF--GREALPVG----ASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLP 360

Query: 371 PAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVR-RRVKLPNAAELTPGFDLCVNA 430
             V+++ E G+GG V+D+GT +T L   AY       + +   LP A+ ++  FD C + 
Sbjct: 361 DGVFDLTETGDGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSI-FDTCYDL 420

Query: 431 SGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEE-GVMCLAIRAVESGNGFSVIGNLMQ 457
           SG      +P + F    G V   P RN+ +  ++ G  C A  A  S  G S+IGN+ Q
Sbjct: 421 SG-FVSVRVPTVSFYFTEGPVLTLPARNFLMPVDDSGTYCFAFAA--SPTGLSIIGNIQQ 470

BLAST of MELO3C005359.jh1 vs. ExPASy Swiss-Prot
Match: Q9LTW4 (Aspartic proteinase NANA, chloroplast OS=Arabidopsis thaliana OX=3702 GN=NANA PE=1 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 8.1e-50
Identity = 144/431 (33.41%), Postives = 206/431 (47.80%), Query Frame = 0

Query: 38  LKLPLLHK-----PPFSSPSQSLSSDTHRLSLLFSRPNPT--LKSPLISGASTGSGQYFV 97
           ++L L H+      P S     + +D  R SL+  + N T  +K  L SG   G+ QYF 
Sbjct: 49  VRLKLAHRDTLLPKPLSRIEDVIGADQKRHSLISRKRNSTVGVKMDLGSGIDYGTAQYFT 108

Query: 98  DIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCR 157
           +IR+GTP +   +V DTGS+L WV C              F    S SF    C    C+
Sbjct: 109 EIRVGTPAKKFRVVVDTGSELTWVNCR--YRARGKDNRRVFRADESKSFKTVGCLTQTCK 168

Query: 158 LLPHAPPHHCNHTLLHSPCRFLYSYADGSLSSGFFSKETTTLKTLSGSEIHLKGLSFGCG 217
           +               +PC + Y YADGS + G F+KET T+   +G    L G   GC 
Sbjct: 169 VDLMNLFSLTTCPTPSTPCSYDYRYADGSAAQGVFAKETITVGLTNGRMARLPGHLIGC- 228

Query: 218 FRISGPSVSGAQFNGARGVMGLGRGSISFSSQLGRRFGNKFSYCLMDYTLSPPPTSFLMI 277
                 S +G  F GA GV+GL     SF+S     +G KFSYCL+D+  +   +++L+ 
Sbjct: 229 ----SSSFTGQSFQGADGVLGLAFSDFSFTSTATSLYGAKFSYCLVDHLSNKNVSNYLIF 288

Query: 278 GGGLHSLPVNNATKISY---TPLQINPLSPTFYYITINSITIDGVKLPINPAVWEIDEQG 337
           G        + +TK ++   TPL +  + P FY I +  I++    L I   VW  D   
Sbjct: 289 GS-------SRSTKTAFRRTTPLDLTRI-PPFYAINVIGISLGYDMLDIPSQVW--DATS 348

Query: 338 NGGTVVDSGTTLTYLTKTAYEEVLKSVRR-RVKLPNAAELTPGFDLCVNASGESRRPSLP 397
            GGT++DSGT+LT L   AY++V+  + R  V+L          + C + +       LP
Sbjct: 349 GGGTILDSGTSLTLLADAAYKQVVTGLARYLVELKRVKPEGVPIEYCFSFTSGFNVSKLP 408

Query: 398 RLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVESGN-GFSVIGNLMQQGFLLEFDKE 457
           +L F L GGA F P  ++Y ++   GV CL    V +G    +VIGN+MQQ +L EFD  
Sbjct: 409 QLTFHLKGGARFEPHRKSYLVDAAPGVKCLGF--VSAGTPATNVIGNIMQQNYLWEFDLM 460

BLAST of MELO3C005359.jh1 vs. ExPASy TrEMBL
Match: A0A1S3CSZ8 (aspartyl protease family protein 2 OS=Cucumis melo OX=3656 GN=LOC103504612 PE=3 SV=1)

HSP 1 Score: 932 bits (2409), Expect = 0.0
Identity = 459/459 (100.00%), Postives = 459/459 (100.00%), Query Frame = 0

Query: 1   MPMLSISPFFLLIPLFFFFLTHLSNPNATAVAAAADFLKLPLLHKPPFSSPSQSLSSDTH 60
           MPMLSISPFFLLIPLFFFFLTHLSNPNATAVAAAADFLKLPLLHKPPFSSPSQSLSSDTH
Sbjct: 1   MPMLSISPFFLLIPLFFFFLTHLSNPNATAVAAAADFLKLPLLHKPPFSSPSQSLSSDTH 60

Query: 61  RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR 120
           RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR
Sbjct: 61  RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR 120

Query: 121 NCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHAPPHHCNHTLLHSPCRFLYSYADGSL 180
           NCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHAPPHHCNHTLLHSPCRFLYSYADGSL
Sbjct: 121 NCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHAPPHHCNHTLLHSPCRFLYSYADGSL 180

Query: 181 SSGFFSKETTTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS 240
           SSGFFSKETTTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS
Sbjct: 181 SSGFFSKETTTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS 240

Query: 241 SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPVNNATKISYTPLQINPLSPTFY 300
           SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPVNNATKISYTPLQINPLSPTFY
Sbjct: 241 SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPVNNATKISYTPLQINPLSPTFY 300

Query: 301 YITINSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL 360
           YITINSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL
Sbjct: 301 YITINSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL 360

Query: 361 PNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA 420
           PNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA
Sbjct: 361 PNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA 420

Query: 421 VESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459
           VESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP
Sbjct: 421 VESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459

BLAST of MELO3C005359.jh1 vs. ExPASy TrEMBL
Match: A0A0A0KNH6 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G174650 PE=3 SV=1)

HSP 1 Score: 900 bits (2327), Expect = 0.0
Identity = 446/459 (97.17%), Postives = 450/459 (98.04%), Query Frame = 0

Query: 1   MPMLSISPFFLLIPLFFFFLTHLSNPNATAVAAAADFLKLPLLHKPPFSSPSQSLSSDTH 60
           MP+LSISPFFLLI LF FFLTHL NPNATAVAA ADFLKLPLLHKPPFSSPSQSLSSDTH
Sbjct: 1   MPVLSISPFFLLILLFSFFLTHLPNPNATAVAAPADFLKLPLLHKPPFSSPSQSLSSDTH 60

Query: 61  RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR 120
           RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR
Sbjct: 61  RLSLLFSRPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR 120

Query: 121 NCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHAPPHHCNHTLLHSPCRFLYSYADGSL 180
           NCSHHPPSSAF PRHSSSFSPFHCFDPHCRLLPHAP H CNHT LHSPCRFLYSYADGSL
Sbjct: 121 NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSL 180

Query: 181 SSGFFSKETTTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS 240
           SSGFFSKETTTLK+LSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS
Sbjct: 181 SSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS 240

Query: 241 SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPVNNATKISYTPLQINPLSPTFY 300
           SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLP+ NATKISYTPLQINPLSPTFY
Sbjct: 241 SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFY 300

Query: 301 YITINSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL 360
           YITI+SITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL
Sbjct: 301 YITIHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL 360

Query: 361 PNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA 420
           PNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA
Sbjct: 361 PNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA 420

Query: 421 VESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459
           VESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP
Sbjct: 421 VESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459

BLAST of MELO3C005359.jh1 vs. ExPASy TrEMBL
Match: A0A6J1HXS2 (aspartyl protease family protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111469001 PE=3 SV=1)

HSP 1 Score: 792 bits (2045), Expect = 3.08e-287
Identity = 398/461 (86.33%), Postives = 416/461 (90.24%), Query Frame = 0

Query: 3   MLSISPFFLLIPLFFFFLTHLSNPNATAVAAAADFLKLPLLHKPPFSSPSQSLSSDTHRL 62
           MLS+S FF LI L  F L  L N      AAAAD+LKLPLLHK PFSSPSQ+LSSDTHRL
Sbjct: 5   MLSVSTFFHLI-LLLFSLADLLN-----AAAAADYLKLPLLHKNPFSSPSQALSSDTHRL 64

Query: 63  SLLFS----RPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSA 122
           SLLFS    RPNPTLKSPLISGASTGSGQYFVD+R+GTPPQSLLLVADTGSDLVWVKCSA
Sbjct: 65  SLLFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSA 124

Query: 123 CRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHAPPHHCNHTLLHSPCRFLYSYADG 182
           CRNCSHHPPSSAF PRHSSSFSPFHCFDPHCRLLPHAPPH CNHT LHSPCRFLY+YADG
Sbjct: 125 CRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADG 184

Query: 183 SLSSGFFSKETTTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSIS 242
           S SSGFFSKETTTLKTLSGSE  LK LSFGCGFRISGPSVSGAQFNGARGVMGLGRG IS
Sbjct: 185 STSSGFFSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPIS 244

Query: 243 FSSQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPVNNATKISYTPLQINPLSPT 302
           FS+QLGRRFGNKFSYCLMDYTLSPPPTS+LMIGGGL SLPV NATKISYTPL INPLSPT
Sbjct: 245 FSTQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPT 304

Query: 303 FYYITINSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRV 362
           FYYI + SIT+DGVKLPINP VW IDEQGNGGTVVDSGTTLTYL + AY+EVLK+VR+RV
Sbjct: 305 FYYIAVKSITVDGVKLPINPTVWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAVRQRV 364

Query: 363 KLPNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAI 422
           KLP AAELTPGFDLCVN S ES+RPSLPR+RFR+G GAVFAPP RNYFLET EGVMCLAI
Sbjct: 365 KLPAAAELTPGFDLCVNVSKESQRPSLPRVRFRVGNGAVFAPPARNYFLETVEGVMCLAI 424

Query: 423 RAVESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459
           RAVE GNGFSVIGNLMQQGFLLEFDKE SRLGF+RRGCGLP
Sbjct: 425 RAVEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

BLAST of MELO3C005359.jh1 vs. ExPASy TrEMBL
Match: A0A6J1ELQ4 (aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111435697 PE=3 SV=1)

HSP 1 Score: 791 bits (2042), Expect = 8.81e-287
Identity = 395/461 (85.68%), Postives = 416/461 (90.24%), Query Frame = 0

Query: 3   MLSISPFFLLIPLFFFFLTHLSNPNATAVAAAADFLKLPLLHKPPFSSPSQSLSSDTHRL 62
           MLS+SPFF LI L  F L  L N      AAAAD+LKLPLLHK PFSSPSQ+LSSDTHRL
Sbjct: 5   MLSVSPFFHLI-LLLFSLADLLN-----AAAAADYLKLPLLHKNPFSSPSQALSSDTHRL 64

Query: 63  SLLFS----RPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSA 122
           SLLFS    RPNPTLKSPLISGASTGSGQYFVD+R+GTPPQSLLLVADTGSDLVWVKCSA
Sbjct: 65  SLLFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSA 124

Query: 123 CRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHAPPHHCNHTLLHSPCRFLYSYADG 182
           CRNCSHHPPSSAF PRHSSSFSPFHCFDPHCRLLPHAPPH CNHT LHSPCRFLY+YADG
Sbjct: 125 CRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADG 184

Query: 183 SLSSGFFSKETTTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSIS 242
           S SSGFFSKETTTLKTL+GSE  LK LSFGCGFRISGPSVSGAQFNGARGVMGLGRG IS
Sbjct: 185 STSSGFFSKETTTLKTLTGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPIS 244

Query: 243 FSSQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPVNNATKISYTPLQINPLSPT 302
           FS+QLGRRFGNKFSYCLMDYTLSPPPTS+LMIGGGL  LPV NATKISYTPL INPLSPT
Sbjct: 245 FSTQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRRLPVTNATKISYTPLLINPLSPT 304

Query: 303 FYYITINSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRV 362
           FYYI + SIT+DGVKLPINP +W IDEQGNGGTVVDSGTTLTYL + AY+EVLK++R+RV
Sbjct: 305 FYYIAVKSITVDGVKLPINPTLWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRV 364

Query: 363 KLPNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAI 422
           KLP AAELTPGFDLCVN S ES+RPSLPR+RF+LG GAVF PP RNYFLETEEGVMCLAI
Sbjct: 365 KLPAAAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFPPPARNYFLETEEGVMCLAI 424

Query: 423 RAVESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459
           RAVE GNGFSVIGNLMQQGFLLEFDKE SRLGF+RRGCGLP
Sbjct: 425 RAVEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

BLAST of MELO3C005359.jh1 vs. ExPASy TrEMBL
Match: A0A6J1EPZ3 (aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111436585 PE=3 SV=1)

HSP 1 Score: 733 bits (1892), Expect = 5.28e-264
Identity = 364/458 (79.48%), Postives = 394/458 (86.03%), Query Frame = 0

Query: 6   ISPFFLLIPLFFFFLTHLSNPNATAVAAAADFLKLPLLHKPPFSSPSQSLSSDTHRLSLL 65
           +  F LL+ L    L  LSN      A  + +LK PLLH  PFSSPSQ+LSSDTHRLSLL
Sbjct: 4   VPQFLLLLLLLLSSLADLSN------AIPSQYLKFPLLHTNPFSSPSQALSSDTHRLSLL 63

Query: 66  FS--RPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCS 125
           FS  R +PTLKSPLISGASTGSGQYFV++ LGTPPQSLLLV DTGSDLVWVKCS CRNCS
Sbjct: 64  FSAHRHSPTLKSPLISGASTGSGQYFVNLHLGTPPQSLLLVVDTGSDLVWVKCSPCRNCS 123

Query: 126 HHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHAPPHHCNHTLLHSPCRFLYSYADGSLSSG 185
           HHPPSSAFFPRHSSSFSPFHCFDPHCRLLPH P H CNHT LHSPC FLYSYAD SLSSG
Sbjct: 124 HHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPCSFLYSYADSSLSSG 183

Query: 186 FFSKETTTLKTLSGS--EIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSS 245
           FFSK+ TT  T SG+  +  L  LSFGCGFRISGPSVSGA+F GARGVMGLGRG ISFSS
Sbjct: 184 FFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFTGARGVMGLGRGPISFSS 243

Query: 246 QLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPVNNATKISYTPLQINPLSPTFYY 305
           QLG RFGN FSYCLMDYTLSPPPTS+LMIGGGL SLPV NA+KISYTPLQINPLSPTFYY
Sbjct: 244 QLGHRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYY 303

Query: 306 ITINSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLP 365
           I + SIT+DGVKLPINP VW IDEQGNGGTVVDSGTTLTYL + AYEEVLK++RRRVKLP
Sbjct: 304 IVVKSITVDGVKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLP 363

Query: 366 NAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAV 425
            A +L+PGFDLCVNAS ESR  SLP++RFR+GGG VFAPP RNYF+ETEEGVMCLAIR V
Sbjct: 364 RALQLSPGFDLCVNASSESRMRSLPQIRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPV 423

Query: 426 ESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459
           +SGNGFSVIGNLMQQGFLLEFD+E+SR+GF+RRGCGLP
Sbjct: 424 DSGNGFSVIGNLMQQGFLLEFDREKSRMGFSRRGCGLP 455

BLAST of MELO3C005359.jh1 vs. TAIR 10
Match: AT3G25700.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 595.5 bits (1534), Expect = 3.6e-170
Identity = 296/459 (64.49%), Postives = 355/459 (77.34%), Query Frame = 0

Query: 12  LIPLFFFFLTHLS-----NPNATAVAAAADFLKLPLLHKPPFSSPSQSLSSDTHRLSLLF 71
           + PL FF  + LS       N  AV+    +LKLPLL K PF SP+Q+L+ DT RL  L 
Sbjct: 1   MFPLIFFLCSFLSLFLLPPSNIAAVSNHNKYLKLPLLRKSPFPSPTQALALDTRRLHFLS 60

Query: 72  SR--PNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSH 131
            R  P P +KSP++SGA++GSGQYFVD+R+G PPQSLLL+ADTGSDLVWVKCSACRNCSH
Sbjct: 61  LRRKPIPFVKSPVVSGAASGSGQYFVDLRIGQPPQSLLLIADTGSDLVWVKCSACRNCSH 120

Query: 132 HPPSSAFFPRHSSSFSPFHCFDPHCRLLP---HAPPHHCNHTLLHSPCRFLYSYADGSLS 191
           H P++ FFPRHSS+FSP HC+DP CRL+P    AP   CNHT +HS C + Y YADGSL+
Sbjct: 121 HSPATVFFPRHSSTFSPAHCYDPVCRLVPKPDRAP--ICNHTRIHSTCHYEYGYADGSLT 180

Query: 192 SGFFSKETTTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSS 251
           SG F++ETT+LKT SG E  LK ++FGCGFRISG SVSG  FNGA GVMGLGRG ISF+S
Sbjct: 181 SGLFARETTSLKTSSGKEARLKSVAFGCGFRISGQSVSGTSFNGANGVMGLGRGPISFAS 240

Query: 252 QLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPVNNATKISYTPLQINPLSPTFYY 311
           QLGRRFGNKFSYCLMDYTLSPPPTS+L+IG G      +  +K+ +TPL  NPLSPTFYY
Sbjct: 241 QLGRRFGNKFSYCLMDYTLSPPPTSYLIIGNG-----GDGISKLFFTPLLTNPLSPTFYY 300

Query: 312 ITINSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLP 371
           + + S+ ++G KL I+P++WEID+ GNGGTVVDSGTTL +L + AY  V+ +VRRRVKLP
Sbjct: 301 VKLKSVFVNGAKLRIDPSIWEIDDSGNGGTVVDSGTTLAFLAEPAYRSVIAAVRRRVKLP 360

Query: 372 NAAELTPGFDLCVNASGESR-RPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA 431
            A  LTPGFDLCVN SG ++    LPRL+F   GGAVF PPPRNYF+ETEE + CLAI++
Sbjct: 361 IADALTPGFDLCVNVSGVTKPEKILPRLKFEFSGGAVFVPPPRNYFIETEEQIQCLAIQS 420

Query: 432 VESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 460
           V+   GFSVIGNLMQQGFL EFD++ SRLGF+RRGC LP
Sbjct: 421 VDPKVGFSVIGNLMQQGFLFEFDRDRSRLGFSRRGCALP 452

BLAST of MELO3C005359.jh1 vs. TAIR 10
Match: AT3G25700.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 406.0 bits (1042), Expect = 4.1e-113
Identity = 227/459 (49.46%), Postives = 270/459 (58.82%), Query Frame = 0

Query: 12  LIPLFFFFLTHLS-----NPNATAVAAAADFLKLPLLHKPPFSSPSQSLSSDTHRLSLLF 71
           + PL FF  + LS       N  AV+    +LKLPLL K PF SP+Q+L+ DT RL  L 
Sbjct: 1   MFPLIFFLCSFLSLFLLPPSNIAAVSNHNKYLKLPLLRKSPFPSPTQALALDTRRLHFLS 60

Query: 72  SR--PNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSH 131
            R  P P +KSP++SGA++GSGQYFVD+R+G PPQSLLL+ADTGSDLVWVKCSACRNCSH
Sbjct: 61  LRRKPIPFVKSPVVSGAASGSGQYFVDLRIGQPPQSLLLIADTGSDLVWVKCSACRNCSH 120

Query: 132 HPPSSAFFPRHSSSFSPFHCFDPHCRLLP---HAPPHHCNHTLLHSPCRFLYSYADGSLS 191
           H P++ FFPRHSS+FSP HC+DP CRL+P    AP   CNHT +HS C + Y YADGSL+
Sbjct: 121 HSPATVFFPRHSSTFSPAHCYDPVCRLVPKPDRAP--ICNHTRIHSTCHYEYGYADGSLT 180

Query: 192 SGFFSKETTTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSS 251
           SG F++ETT+LKT SG E  LK ++FGCGFRISG SVS                      
Sbjct: 181 SGLFARETTSLKTSSGKEARLKSVAFGCGFRISGQSVS---------------------- 240

Query: 252 QLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPVNNATKISYTPLQINPLSPTFYY 311
                                                                       
Sbjct: 241 ------------------------------------------------------------ 300

Query: 312 ITINSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLP 371
                                    GNGGTVVDSGTTL +L + AY  V+ +VRRRVKLP
Sbjct: 301 -------------------------GNGGTVVDSGTTLAFLAEPAYRSVIAAVRRRVKLP 350

Query: 372 NAAELTPGFDLCVNASGESR-RPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA 431
            A  LTPGFDLCVN SG ++    LPRL+F   GGAVF PPPRNYF+ETEE + CLAI++
Sbjct: 361 IADALTPGFDLCVNVSGVTKPEKILPRLKFEFSGGAVFVPPPRNYFIETEEQIQCLAIQS 350

Query: 432 VESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 460
           V+   GFSVIGNLMQQGFL EFD++ SRLGF+RRGC LP
Sbjct: 421 VDPKVGFSVIGNLMQQGFLFEFDRDRSRLGFSRRGCALP 350

BLAST of MELO3C005359.jh1 vs. TAIR 10
Match: AT2G42980.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 236.1 bits (601), Expect = 5.6e-62
Identity = 153/393 (38.93%), Postives = 207/393 (52.67%), Query Frame = 0

Query: 73  LKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFF 132
           L + L SG + GSG+YF+D+ +GTPP+   L+ DTGSDL W++C  C +C H   +  F+
Sbjct: 145 LIATLESGMTLGSGEYFMDVLVGTPPKHFSLILDTGSDLNWLQCLPCYDCFHQ--NGMFY 204

Query: 133 -PRHSSSFSPFHCFDPHCRLLPHA-PPHHCNHTLLHSPCRFLYSYADGSLSSGFFSKETT 192
            P+ S+SF    C DP C L+    PP  C     +  C + Y Y D S ++G F+ ET 
Sbjct: 205 DPKTSASFKNITCNDPRCSLISSPDPPVQCESD--NQSCPYFYWYGDRSNTTGDFAVETF 264

Query: 193 T--LKTLSG--SEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQLGRR 252
           T  L T  G  SE  +  + FGCG    G       F+GA G++GLGRG +SFSSQL   
Sbjct: 265 TVNLTTTEGGSSEYKVGNMMFGCGHWNRG------LFSGASGLLGLGRGPLSFSSQLQSL 324

Query: 253 FGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPVNNATKISYTPLQINPLSPTFYYITINS 312
           +G+ FSYCL+D   +   +S L+ G     L   N    S+   + N +  TFYYI I S
Sbjct: 325 YGHSFSYCLVDRNSNTNVSSKLIFGEDKDLLNHTNLNFTSFVNGKENSVE-TFYYIQIKS 384

Query: 313 ITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVK--LPNAA 372
           I + G  L I    W I   G+GGT++DSGTTL+Y  + AYE +      ++K   P   
Sbjct: 385 ILVGGKALDIPEETWNISSDGDGGTIIDSGTTLSYFAEPAYEIIKNKFAEKMKENYPIFR 444

Query: 373 ELTPGFDLCVNASG-ESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVES 432
           +  P  D C N SG E     LP L      G V+  P  N F+   E ++CLAI     
Sbjct: 445 DF-PVLDPCFNVSGIEENNIHLPELGIAFVDGTVWNFPAENSFIWLSEDLVCLAILGTPK 504

Query: 433 GNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGC 457
              FS+IGN  QQ F + +D + SRLGFT   C
Sbjct: 505 ST-FSIIGNYQQQNFHILYDTKRSRLGFTPTKC 524

BLAST of MELO3C005359.jh1 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 228.0 bits (580), Expect = 1.5e-59
Identity = 140/386 (36.27%), Postives = 206/386 (53.37%), Query Frame = 0

Query: 75  SPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFFPR 134
           S ++SG S GSG+YF  + +GTP + + +V DTGSD+VW++C+ CR C +      F PR
Sbjct: 129 SSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRC-YSQSDPIFDPR 188

Query: 135 HSSSFSPFHCFDPHCRLLPHAPPHHCNHTLLHSPCRFLYSYADGSLSSGFFSKETTTLKT 194
            S +++   C  PHCR L  A    CN       C +  SY DGS + G FS ET T + 
Sbjct: 189 KSKTYATIPCSSPHCRRLDSA---GCN--TRRKTCLYQVSYGDGSFTVGDFSTETLTFR- 248

Query: 195 LSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQLGRRFGNKFSYC 254
                  +KG++ GCG    G       F GA G++GLG+G +SF  Q G RF  KFSYC
Sbjct: 249 ----RNRVKGVALGCGHDNEG------LFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYC 308

Query: 255 LMDYTLSPPPTSFLMIGGGLHSLPVNNATKISYTPLQINPLSPTFYYITINSITIDGVKL 314
           L+D + S  P+S +     +  +         +TPL  NP   TFYY+ +  I++ G ++
Sbjct: 309 LVDRSASSKPSSVVFGNAAVSRI-------ARFTPLLSNPKLDTFYYVGLLGISVGGTRV 368

Query: 315 P-INPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLPNAAELTPGFDLC 374
           P +  +++++D+ GNGG ++DSGT++T L + AY  +  + R   K    A     FD C
Sbjct: 369 PGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTC 428

Query: 375 VNAS--GESRRPSLPRLRFRLGGGAVFAPPPRNYFLETE-EGVMCLAIRAVESGNGFSVI 434
            + S   E + P++  L FR   GA  + P  NY +  +  G  C A     +  G S+I
Sbjct: 429 FDLSNMNEVKVPTVV-LHFR---GADVSLPATNYLIPVDTNGKFCFAFAG--TMGGLSII 484

Query: 435 GNLMQQGFLLEFDKEESRLGFTRRGC 457
           GN+ QQGF + +D   SR+GF   GC
Sbjct: 489 GNIQQQGFRVVYDLASSRVGFAPGGC 484

BLAST of MELO3C005359.jh1 vs. TAIR 10
Match: AT3G59080.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 224.2 bits (570), Expect = 2.2e-58
Identity = 146/392 (37.24%), Postives = 205/392 (52.30%), Query Frame = 0

Query: 73  LKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFF 132
           L + L SG + GSG+YF+D+ +G+PP+   L+ DTGSDL W++C  C +C     + AF+
Sbjct: 155 LVATLESGMTLGSGEYFMDVLVGSPPKHFSLILDTGSDLNWIQCLPCYDCFQQ--NGAFY 214

Query: 133 -PRHSSSFSPFHCFDPHCRLLPHA-PPHHCNHTLLHSPCRFLYSYADGSLSSGFFSKETT 192
            P+ S+S+    C D  C L+    PP  C     +  C + Y Y D S ++G F+ ET 
Sbjct: 215 DPKASASYKNITCNDQRCNLVSSPDPPMPCKSD--NQSCPYYYWYGDSSNTTGDFAVETF 274

Query: 193 T--LKTLSGSE--IHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQLGRR 252
           T  L T  GS    +++ + FGCG    G       F+GA G++GLGRG +SFSSQL   
Sbjct: 275 TVNLTTNGGSSELYNVENMMFGCGHWNRG------LFHGAAGLLGLGRGPLSFSSQLQSL 334

Query: 253 FGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPVNNATKISYTPLQINPLSPTFYYITINS 312
           +G+ FSYCL+D       +S L+ G     L   N    S+   + N L  TFYY+ I S
Sbjct: 335 YGHSFSYCLVDRNSDTNVSSKLIFGEDKDLLSHPNLNFTSFVAGKEN-LVDTFYYVQIKS 394

Query: 313 ITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRV--KLPNAA 372
           I + G  L I    W I   G GGT++DSGTTL+Y  + AYE +   +  +   K P   
Sbjct: 395 ILVAGEVLNIPEETWNISSDGAGGTIIDSGTTLSYFAEPAYEFIKNKIAEKAKGKYPVYR 454

Query: 373 ELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVESG 432
           +  P  D C N SG      LP L      GAV+  P  N F+   E ++CLA+      
Sbjct: 455 DF-PILDPCFNVSG-IHNVQLPELGIAFADGAVWNFPTENSFIWLNEDLVCLAMLGTPK- 514

Query: 433 NGFSVIGNLMQQGFLLEFDKEESRLGFTRRGC 457
           + FS+IGN  QQ F + +D + SRLG+    C
Sbjct: 515 SAFSIIGNYQQQNFHILYDTKRSRLGYAPTKC 532

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008467208.10.0100.00PREDICTED: aspartyl protease family protein 2 [Cucumis melo][more]
XP_004143702.10.097.17aspartyl protease family protein 2 [Cucumis sativus] >KGN50439.1 hypothetical pr... [more]
XP_038907006.14.91e-30089.50aspartyl protease family protein 2 [Benincasa hispida][more]
XP_023549997.12.71e-28886.33aspartyl protease family protein 2 [Cucurbita pepo subsp. pepo][more]
KAG7017139.15.47e-28785.90Aspartic proteinase nepenthesin-2, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
Q9LNJ32.1e-5836.27Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Q766C29.3e-5433.96Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
Q766C33.3e-5131.47Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Q9LHE33.6e-5030.94Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LTW48.1e-5033.41Aspartic proteinase NANA, chloroplast OS=Arabidopsis thaliana OX=3702 GN=NANA PE... [more]
Match NameE-valueIdentityDescription
A0A1S3CSZ80.0100.00aspartyl protease family protein 2 OS=Cucumis melo OX=3656 GN=LOC103504612 PE=3 ... [more]
A0A0A0KNH60.097.17Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G17465... [more]
A0A6J1HXS23.08e-28786.33aspartyl protease family protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111469001 P... [more]
A0A6J1ELQ48.81e-28785.68aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1EPZ35.28e-26479.48aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT3G25700.13.6e-17064.49Eukaryotic aspartyl protease family protein [more]
AT3G25700.24.1e-11349.46Eukaryotic aspartyl protease family protein [more]
AT2G42980.15.6e-6238.93Eukaryotic aspartyl protease family protein [more]
AT1G01300.11.5e-5936.27Eukaryotic aspartyl protease family protein [more]
AT3G59080.12.2e-5837.24Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Harukei-3) v1.41
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 47..228
e-value: 3.3E-51
score: 175.6
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 1..42
e-value: 1.3E-6
score: 30.5
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 1..227
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 70..223
e-value: 1.7E-37
score: 128.7
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 1..43
e-value: 1.0E-8
score: 35.6
NoneNo IPR availablePANTHERPTHR47967:SF72BNAA02G27600D PROTEINcoord: 1..228
NoneNo IPR availablePANTHERPTHR47967OS07G0603500 PROTEIN-RELATEDcoord: 1..228
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 1..223
score: 21.379047
IPR034161Pepsin-like domain, plantCDDcd05476pepsin_A_like_plantcoord: 1..227
e-value: 8.47967E-60
score: 187.085

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C005359.jh1.t2MELO3C005359.jh1.t2mRNA
MELO3C005359.jh1.t1MELO3C005359.jh1.t1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity