MELO3C000140 (gene) Melon (DHL92) v4

Overview
NameMELO3C000140
Typegene
OrganismCucumis melo cv. DHL92 (Melon (DHL92) v4)
DescriptionHigh-affinity nitrate transporter 2.2
Locationchr12: 5256421 .. 5257947 (+)
RNA-Seq ExpressionMELO3C000140
SyntenyMELO3C000140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAACCTTCCACCTTTCTTGGATTTCCTTCTTCACATGCTTTGTGTCGACGTTCGCAGCTGCCCCTCTCGTCCCCATCATTCGCGACAACCTGAACCTGACGAAAGTCGACATCGGGAACGCTGGAGTTACATCTGTTTCTGGGAGTATCTTCTCTCGTCTTGTGATGGGGGCTGTGTGCGACCTTCTGGGACCACGATATGGATGTGCGTTTTTGATAATGTTATCGGCTCCAACGGTGTTTTGTATGTCGTTTGTGTCGAATGCTGCTGGTTACATAGCGGTTAGATTCATGATCGGGTTTTCGCTAGCAACGTTCGTGTCGTGTCAGTATTGGATGAGTACAATGTTTAATAGTCAGATAATAGGGCTTGTGAACGGGACAGCAGCTGGGTGGGGGAATATGGGAGGAGGAGCAACTCAATTAATAATGCCATTGTTGTATGATGTTATTCAGAGAGCTGGTGCTACTCCTTTTACTGCTTGGAGGATCGCTTTCTTCATTCCTGGTTTCCTTCATGTTCTTATGGGCATTTTGGTTTTGACTCTTGGTCAAGACTTGCCTGATGGTAACCTTGCTAGTCTTCAGAAGAAGGGAAATGTTGCCAAAGACAAATTCTCCAATGTATGTATAGTTAGCATCTCTTCTTAATAATATAATCTCTCTCATATATATGCTTTACTTAATTAATCATCATCTCTTATAATAAACTATATATCCTCTTCCTCACAGGTCTTGTGGTATGCTGTCACCAATTATCGAACTTGGATCTTCGTCCTCCTCTATGGCTACTCCATGGGAGTTGAGCTTTCCACCGATAATGTCATTGCCGAATATTTCTACGATAGGTAACATAATTAAATTATCACTGTATCGTTGTTGTGATTTTAAATAACAGTGTGTTAGCTACACAAACAAAGCAAATCGAAGAAGTCCTTATGTTTGTCTGTGTTTGTTAAAACAGGTTTGATCTAAAGCTTCACACAGCAGGAATCATTGCAGCAACGTTCGGGATGGCGAATCTAGTGGCTAGACCGTTTGGAGGGTATGCATCGGACGTAGCAGCGCGATACTTCGGGATGAGAGGAAGGTTGTGGACACTATGGATCCTACAAACAGCTGGGGGAGTGTTTTGCATGTGGCTAGGGAAAGCAACCAAGTTGCCAATAGCAATAGTGGCAATGATATTGTTCTCAATAGGAGCACAAGCAGCATGTGGAGCCACATTTGGTATAATTCCATTCATATCAAGGAGATCTCTAGGAATAATATCAGGACTAACAGGAGCTGGTGGGAACTTTGGAAGTGGTTTAACACAATTAGTATTCTTCTCAACAACAAAATACTCAACAGACACAGGATTGTTCTTGATGGGAATAATGATTGTTTGTTGCACTCTTCCAATAACTTTGGTTCATTTCCCTCAATGGGGAAGCATGTTTCTACCACCAACTAAACATGTTGAAAAATCAACTGAAGAATTTTATTATGGTTCTGAATGGACTGAGGATGAAAAGAAGAAAG

mRNA sequence

ATGAGAACCTTCCACCTTTCTTGGATTTCCTTCTTCACATGCTTTGTGTCGACGTTCGCAGCTGCCCCTCTCGTCCCCATCATTCGCGACAACCTGAACCTGACGAAAGTCGACATCGGGAACGCTGGAGTTACATCTGTTTCTGGGAGTATCTTCTCTCGTCTTGTGATGGGGGCTGTGTGCGACCTTCTGGGACCACGATATGGATGTGCGTTTTTGATAATGTTATCGGCTCCAACGGTGTTTTGTATGTCGTTTGTGTCGAATGCTGCTGGTTACATAGCGGTTAGATTCATGATCGGGTTTTCGCTAGCAACGTTCGTGTCGTGTCAGTATTGGATGAGTACAATGTTTAATAGTCAGATAATAGGGCTTGTGAACGGGACAGCAGCTGGGTGGGGGAATATGGGAGGAGGAGCAACTCAATTAATAATGCCATTGTTGTATGATGTTATTCAGAGAGCTGGTGCTACTCCTTTTACTGCTTGGAGGATCGCTTTCTTCATTCCTGGTTTCCTTCATGTTCTTATGGGCATTTTGGTTTTGACTCTTGGTCAAGACTTGCCTGATGGTAACCTTGCTAGTCTTCAGAAGAAGGGAAATGTTGCCAAAGACAAATTCTCCAATGTCTTGTGGTATGCTGTCACCAATTATCGAACTTGGATCTTCGTCCTCCTCTATGGCTACTCCATGGGAGTTGAGCTTTCCACCGATAATGTCATTGCCGAATATTTCTACGATAGGTTTGATCTAAAGCTTCACACAGCAGGAATCATTGCAGCAACGTTCGGGATGGCGAATCTAGTGGCTAGACCGTTTGGAGGGTATGCATCGGACGTAGCAGCGCGATACTTCGGGATGAGAGGAAGGTTGTGGACACTATGGATCCTACAAACAGCTGGGGGAGTGTTTTGCATGTGGCTAGGGAAAGCAACCAAGTTGCCAATAGCAATAGTGGCAATGATATTGTTCTCAATAGGAGCACAAGCAGCATGTGGAGCCACATTTGGTATAATTCCATTCATATCAAGGAGATCTCTAGGAATAATATCAGGACTAACAGGAGCTGGTGGGAACTTTGGAAGTGGTTTAACACAATTAGTATTCTTCTCAACAACAAAATACTCAACAGACACAGGATTGTTCTTGATGGGAATAATGATTGTTTGTTGCACTCTTCCAATAACTTTGGTTCATTTCCCTCAATGGGGAAGCATGTTTCTACCACCAACTAAACATGTTGAAAAATCAACTGAAGAATTTTATTATGGTTCTGAATGGACTGAGGATGAAAAGAAGAAAG

Coding sequence (CDS)

ATGAGAACCTTCCACCTTTCTTGGATTTCCTTCTTCACATGCTTTGTGTCGACGTTCGCAGCTGCCCCTCTCGTCCCCATCATTCGCGACAACCTGAACCTGACGAAAGTCGACATCGGGAACGCTGGAGTTACATCTGTTTCTGGGAGTATCTTCTCTCGTCTTGTGATGGGGGCTGTGTGCGACCTTCTGGGACCACGATATGGATGTGCGTTTTTGATAATGTTATCGGCTCCAACGGTGTTTTGTATGTCGTTTGTGTCGAATGCTGCTGGTTACATAGCGGTTAGATTCATGATCGGGTTTTCGCTAGCAACGTTCGTGTCGTGTCAGTATTGGATGAGTACAATGTTTAATAGTCAGATAATAGGGCTTGTGAACGGGACAGCAGCTGGGTGGGGGAATATGGGAGGAGGAGCAACTCAATTAATAATGCCATTGTTGTATGATGTTATTCAGAGAGCTGGTGCTACTCCTTTTACTGCTTGGAGGATCGCTTTCTTCATTCCTGGTTTCCTTCATGTTCTTATGGGCATTTTGGTTTTGACTCTTGGTCAAGACTTGCCTGATGGTAACCTTGCTAGTCTTCAGAAGAAGGGAAATGTTGCCAAAGACAAATTCTCCAATGTCTTGTGGTATGCTGTCACCAATTATCGAACTTGGATCTTCGTCCTCCTCTATGGCTACTCCATGGGAGTTGAGCTTTCCACCGATAATGTCATTGCCGAATATTTCTACGATAGGTTTGATCTAAAGCTTCACACAGCAGGAATCATTGCAGCAACGTTCGGGATGGCGAATCTAGTGGCTAGACCGTTTGGAGGGTATGCATCGGACGTAGCAGCGCGATACTTCGGGATGAGAGGAAGGTTGTGGACACTATGGATCCTACAAACAGCTGGGGGAGTGTTTTGCATGTGGCTAGGGAAAGCAACCAAGTTGCCAATAGCAATAGTGGCAATGATATTGTTCTCAATAGGAGCACAAGCAGCATGTGGAGCCACATTTGGTATAATTCCATTCATATCAAGGAGATCTCTAGGAATAATATCAGGACTAACAGGAGCTGGTGGGAACTTTGGAAGTGGTTTAACACAATTAGTATTCTTCTCAACAACAAAATACTCAACAGACACAGGATTGTTCTTGATGGGAATAATGATTGTTTGTTGCACTCTTCCAATAACTTTGGTTCATTTCCCTCAATGGGGAAGCATGTTTCTACCACCAACTAAACATGTTGAAAAATCAACTGAAGAATTTTATTATGGTTCTGAATGGACTGAGGATGAAAAGAAGAAA

Protein sequence

MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAVCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNSQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGILVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTAGGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPITLVHFPQWGSMFLPPTKHVEKSTEEFYYGSEWTEDEKKK
Homology
BLAST of MELO3C000140 vs. ExPASy Swiss-Prot
Match: Q9FJH8 (High affinity nitrate transporter 2.4 OS=Arabidopsis thaliana OX=3702 GN=NRT2.4 PE=2 SV=1)

HSP 1 Score: 732.3 bits (1889), Expect = 3.3e-210
Identity = 353/434 (81.34%), Postives = 395/434 (91.01%), Query Frame = 0

Query: 1   MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 60
           MRTFHL+WISFFTCF+STFAAAPLVPIIRDNLNLT+ D+GNAGV SVSGSIFSRLVMGAV
Sbjct: 62  MRTFHLAWISFFTCFISTFAAAPLVPIIRDNLNLTRQDVGNAGVASVSGSIFSRLVMGAV 121

Query: 61  CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 120
           CDLLGPRYGCAFL+MLSAPTVF MSFV  A GYI VRFMIGF LATFVSCQYWMSTMFN 
Sbjct: 122 CDLLGPRYGCAFLVMLSAPTVFSMSFVGGAGGYITVRFMIGFCLATFVSCQYWMSTMFNG 181

Query: 121 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 180
           QIIGLVNGTAAGWGNMGGG TQL+MP++Y++I+R G+T FTAWR+AFF+PG++H++MGIL
Sbjct: 182 QIIGLVNGTAAGWGNMGGGVTQLLMPMVYEIIRRLGSTSFTAWRMAFFVPGWMHIIMGIL 241

Query: 181 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 240
           VLTLGQDLPDGN ++L+KKG V KDKFS VLWYA+TNYRTW+FVLLYGYSMGVEL+TDNV
Sbjct: 242 VLTLGQDLPDGNRSTLEKKGAVTKDKFSKVLWYAITNYRTWVFVLLYGYSMGVELTTDNV 301

Query: 241 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTA 300
           IAEYF+DRF LKLHTAGIIAA+FGMAN  ARP GG+ASD+AAR FGMRGRLWTLWI+QT 
Sbjct: 302 IAEYFFDRFHLKLHTAGIIAASFGMANFFARPIGGWASDIAARRFGMRGRLWTLWIIQTL 361

Query: 301 GGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 360
           GG FC+WLG+AT LP A+V MILFS+GAQAACGATF IIPFISRRSLGIISGLTGAGGNF
Sbjct: 362 GGFFCLWLGRATTLPTAVVFMILFSLGAQAACGATFAIIPFISRRSLGIISGLTGAGGNF 421

Query: 361 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPITLVHFPQWGSMFLPPTKHVEKSTEE 420
           GSGLTQLVFFST+ +ST+ GL  MG+MI+ CTLP+TLVHFPQWGSMFLP T+   KSTEE
Sbjct: 422 GSGLTQLVFFSTSTFSTEQGLTWMGVMIMACTLPVTLVHFPQWGSMFLPSTEDEVKSTEE 481

Query: 421 FYYGSEWTEDEKKK 435
           +YY  EWTE EK+K
Sbjct: 482 YYYMKEWTETEKRK 495

BLAST of MELO3C000140 vs. ExPASy Swiss-Prot
Match: O82811 (High-affinity nitrate transporter 2.1 OS=Arabidopsis thaliana OX=3702 GN=NRT2.1 PE=1 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 2.1e-209
Identity = 354/434 (81.57%), Postives = 394/434 (90.78%), Query Frame = 0

Query: 1   MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 60
           MRTFHLSWISF TCFVSTFAAAPLVPIIR+NLNLTK DIGNAGV SVSGSIFSRLVMGAV
Sbjct: 62  MRTFHLSWISFSTCFVSTFAAAPLVPIIRENLNLTKQDIGNAGVASVSGSIFSRLVMGAV 121

Query: 61  CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 120
           CDLLGPRYGCAFL+MLSAPTVF MSFVS+AAG+I VRFMIGF LATFVSCQYWMSTMFNS
Sbjct: 122 CDLLGPRYGCAFLVMLSAPTVFSMSFVSDAAGFITVRFMIGFCLATFVSCQYWMSTMFNS 181

Query: 121 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 180
           QIIGLVNGTAAGWGNMGGG TQL+MP++Y++I+R G+T FTAWRIAFF+PG+LH++MGIL
Sbjct: 182 QIIGLVNGTAAGWGNMGGGITQLLMPIVYEIIRRCGSTAFTAWRIAFFVPGWLHIIMGIL 241

Query: 181 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 240
           VL LGQDLPDGN A+L+K G VAKDKF  +LWYAVTNYRTWIFVLLYGYSMGVELSTDNV
Sbjct: 242 VLNLGQDLPDGNRATLEKAGEVAKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDNV 301

Query: 241 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTA 300
           IAEYF+DRF LKLHTAG+IAA FGMAN  ARP GGYASD AA+YFGMRGRLWTLWI+QTA
Sbjct: 302 IAEYFFDRFHLKLHTAGLIAACFGMANFFARPAGGYASDFAAKYFGMRGRLWTLWIIQTA 361

Query: 301 GGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 360
           GG+FC+WLG+A  L  A+VAM+LFS+GAQAACGATF I+PF+SRR+LGIISGLTGAGGNF
Sbjct: 362 GGLFCVWLGRANTLVTAVVAMVLFSMGAQAACGATFAIVPFVSRRALGIISGLTGAGGNF 421

Query: 361 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPITLVHFPQWGSMFLPPTKHVEKSTEE 420
           GSGLTQL+FFST+ ++T+ GL  MG+MIV CTLP+TLVHFPQWGSMFLPP+    K TE 
Sbjct: 422 GSGLTQLLFFSTSHFTTEQGLTWMGVMIVACTLPVTLVHFPQWGSMFLPPSTDPVKGTEA 481

Query: 421 FYYGSEWTEDEKKK 435
            YYGSEW E EK+K
Sbjct: 482 HYYGSEWNEQEKQK 495

BLAST of MELO3C000140 vs. ExPASy Swiss-Prot
Match: P0DKG9 (High-affinity nitrate transporter 2.1 OS=Oryza sativa subsp. japonica OX=39947 GN=NRT2.1 PE=1 SV=1)

HSP 1 Score: 716.1 bits (1847), Expect = 2.4e-205
Identity = 349/434 (80.41%), Postives = 390/434 (89.86%), Query Frame = 0

Query: 1   MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 60
           MRTFHLSWISFF+CFVSTFAAAPLVPIIRDNLNLTK DIGNAGV SVSGSIFSRL MGA+
Sbjct: 63  MRTFHLSWISFFSCFVSTFAAAPLVPIIRDNLNLTKADIGNAGVASVSGSIFSRLAMGAI 122

Query: 61  CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 120
           CD+LGPRYGCAFLIML+APTVFCMS + +AAGYIAVRF+IGFSLATFVSCQYWMSTMFNS
Sbjct: 123 CDMLGPRYGCAFLIMLAAPTVFCMSLIDSAAGYIAVRFLIGFSLATFVSCQYWMSTMFNS 182

Query: 121 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 180
           +IIGLVNG AAGWGNMGGGATQLIMPL+YDVI++ GATPFTAWR+A+F+PG LHV+MG+L
Sbjct: 183 KIIGLVNGLAAGWGNMGGGATQLIMPLVYDVIRKCGATPFTAWRLAYFVPGTLHVVMGVL 242

Query: 181 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 240
           VLTLGQDLPDGNL SLQKKG+V +D FS VLWYAVTNYRTWIFVLLYGYSMGVEL+TDNV
Sbjct: 243 VLTLGQDLPDGNLRSLQKKGDVNRDSFSRVLWYAVTNYRTWIFVLLYGYSMGVELTTDNV 302

Query: 241 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTA 300
           IAEYFYDRFDL L  AGIIAA+FGMAN+VARP GG  SD+ ARYFGMR RLW +WILQTA
Sbjct: 303 IAEYFYDRFDLDLRVAGIIAASFGMANIVARPTGGLLSDLGARYFGMRARLWNIWILQTA 362

Query: 301 GGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 360
           GG FC+ LG+A+ LP ++V M+LFS  AQAACGA FG+IPF+SRRSLGIISG+TGAGGNF
Sbjct: 363 GGAFCLLLGRASTLPTSVVCMVLFSFCAQAACGAIFGVIPFVSRRSLGIISGMTGAGGNF 422

Query: 361 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPITLVHFPQWGSMFLPPTKHVEKSTEE 420
           G+GLTQL+FF++++YST TGL  MGIMI+ CTLP+ LVHFPQWGSMFLPP    E   EE
Sbjct: 423 GAGLTQLLFFTSSRYSTGTGLEYMGIMIMACTLPVVLVHFPQWGSMFLPPNAGAE---EE 482

Query: 421 FYYGSEWTEDEKKK 435
            YYGSEW+E EK K
Sbjct: 483 HYYGSEWSEQEKSK 493

BLAST of MELO3C000140 vs. ExPASy Swiss-Prot
Match: P0DKH0 (High-affinity nitrate transporter 2.2 OS=Oryza sativa subsp. japonica OX=39947 GN=NRT2.2 PE=1 SV=1)

HSP 1 Score: 716.1 bits (1847), Expect = 2.4e-205
Identity = 349/434 (80.41%), Postives = 390/434 (89.86%), Query Frame = 0

Query: 1   MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 60
           MRTFHLSWISFF+CFVSTFAAAPLVPIIRDNLNLTK DIGNAGV SVSGSIFSRL MGA+
Sbjct: 63  MRTFHLSWISFFSCFVSTFAAAPLVPIIRDNLNLTKADIGNAGVASVSGSIFSRLAMGAI 122

Query: 61  CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 120
           CD+LGPRYGCAFLIML+APTVFCMS + +AAGYIAVRF+IGFSLATFVSCQYWMSTMFNS
Sbjct: 123 CDMLGPRYGCAFLIMLAAPTVFCMSLIDSAAGYIAVRFLIGFSLATFVSCQYWMSTMFNS 182

Query: 121 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 180
           +IIGLVNG AAGWGNMGGGATQLIMPL+YDVI++ GATPFTAWR+A+F+PG LHV+MG+L
Sbjct: 183 KIIGLVNGLAAGWGNMGGGATQLIMPLVYDVIRKCGATPFTAWRLAYFVPGTLHVVMGVL 242

Query: 181 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 240
           VLTLGQDLPDGNL SLQKKG+V +D FS VLWYAVTNYRTWIFVLLYGYSMGVEL+TDNV
Sbjct: 243 VLTLGQDLPDGNLRSLQKKGDVNRDSFSRVLWYAVTNYRTWIFVLLYGYSMGVELTTDNV 302

Query: 241 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTA 300
           IAEYFYDRFDL L  AGIIAA+FGMAN+VARP GG  SD+ ARYFGMR RLW +WILQTA
Sbjct: 303 IAEYFYDRFDLDLRVAGIIAASFGMANIVARPTGGLLSDLGARYFGMRARLWNIWILQTA 362

Query: 301 GGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 360
           GG FC+ LG+A+ LP ++V M+LFS  AQAACGA FG+IPF+SRRSLGIISG+TGAGGNF
Sbjct: 363 GGAFCLLLGRASTLPTSVVCMVLFSFCAQAACGAIFGVIPFVSRRSLGIISGMTGAGGNF 422

Query: 361 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPITLVHFPQWGSMFLPPTKHVEKSTEE 420
           G+GLTQL+FF++++YST TGL  MGIMI+ CTLP+ LVHFPQWGSMFLPP    E   EE
Sbjct: 423 GAGLTQLLFFTSSRYSTGTGLEYMGIMIMACTLPVVLVHFPQWGSMFLPPNAGAE---EE 482

Query: 421 FYYGSEWTEDEKKK 435
            YYGSEW+E EK K
Sbjct: 483 HYYGSEWSEQEKSK 493

BLAST of MELO3C000140 vs. ExPASy Swiss-Prot
Match: Q9LMZ9 (High-affinity nitrate transporter 2.2 OS=Arabidopsis thaliana OX=3702 GN=NRT2.2 PE=2 SV=1)

HSP 1 Score: 706.8 bits (1823), Expect = 1.5e-202
Identity = 341/433 (78.75%), Postives = 394/433 (90.99%), Query Frame = 0

Query: 1   MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 60
           MRTFHLSWISF TCFVSTFAAAPL+PIIR+NLNLTK DIGNAGV SVSGSIFSRLVMGAV
Sbjct: 60  MRTFHLSWISFSTCFVSTFAAAPLIPIIRENLNLTKHDIGNAGVASVSGSIFSRLVMGAV 119

Query: 61  CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 120
           CDLLGPRYGCAFL+MLSAPTVF MSFVS+AAG+I VRFMIGF LATFVSCQYWMSTMFNS
Sbjct: 120 CDLLGPRYGCAFLVMLSAPTVFSMSFVSDAAGFITVRFMIGFCLATFVSCQYWMSTMFNS 179

Query: 121 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 180
           QIIGLVNGTAAGWGNMGGG TQL+MP++Y++I+R G+T FTAWRIAFF+PG+LH++MGIL
Sbjct: 180 QIIGLVNGTAAGWGNMGGGITQLLMPIVYEIIRRCGSTAFTAWRIAFFVPGWLHIIMGIL 239

Query: 181 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 240
           VLTLGQDLP GN A+++K G VAKDKF  +LWYAVTNYRTWIFVLLYGYSMGVELSTDNV
Sbjct: 240 VLTLGQDLPGGNRAAMEKAGEVAKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDNV 299

Query: 241 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTA 300
           IAEYF+DRF LKLHTAGIIAA FGMAN  ARP GG+ASD+AA+ FGMRGRLWTLWI+QT+
Sbjct: 300 IAEYFFDRFHLKLHTAGIIAACFGMANFFARPAGGWASDIAAKRFGMRGRLWTLWIIQTS 359

Query: 301 GGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 360
           GG+FC+WLG+A  L  A+V+M+LFS+GAQAACGATF I+PF+SRR+LGIISGLTGAGGNF
Sbjct: 360 GGLFCVWLGRANTLVTAVVSMVLFSLGAQAACGATFAIVPFVSRRALGIISGLTGAGGNF 419

Query: 361 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPITLVHFPQWGSMFLPPTKHVEKSTEE 420
           GSGLTQLVFFST++++T+ GL  MG+MIV CTLP+TL+HFPQWGSMF PP+     +TE 
Sbjct: 420 GSGLTQLVFFSTSRFTTEEGLTWMGVMIVACTLPVTLIHFPQWGSMFFPPSNDSVDATEH 479

Query: 421 FYYGSEWTEDEKK 434
           +Y G E++++E++
Sbjct: 480 YYVG-EYSKEEQQ 491

BLAST of MELO3C000140 vs. NCBI nr
Match: XP_008467040.1 (PREDICTED: high affinity nitrate transporter 2.4-like, partial [Cucumis melo])

HSP 1 Score: 865.5 bits (2235), Expect = 1.9e-247
Identity = 434/434 (100.00%), Postives = 434/434 (100.00%), Query Frame = 0

Query: 1   MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 60
           MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV
Sbjct: 1   MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 60

Query: 61  CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 120
           CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS
Sbjct: 61  CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 120

Query: 121 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 180
           QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGIL
Sbjct: 121 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 180

Query: 181 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 240
           VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV
Sbjct: 181 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 240

Query: 241 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTA 300
           IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTA
Sbjct: 241 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTA 300

Query: 301 GGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 360
           GGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 301 GGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 360

Query: 361 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPITLVHFPQWGSMFLPPTKHVEKSTEE 420
           GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPITLVHFPQWGSMFLPPTKHVEKSTEE
Sbjct: 361 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPITLVHFPQWGSMFLPPTKHVEKSTEE 420

Query: 421 FYYGSEWTEDEKKK 435
           FYYGSEWTEDEKKK
Sbjct: 421 FYYGSEWTEDEKKK 434

BLAST of MELO3C000140 vs. NCBI nr
Match: TYK21386.1 (high affinity nitrate transporter 2.4-like [Cucumis melo var. makuwa])

HSP 1 Score: 865.5 bits (2235), Expect = 1.9e-247
Identity = 434/434 (100.00%), Postives = 434/434 (100.00%), Query Frame = 0

Query: 1   MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 60
           MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV
Sbjct: 62  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 121

Query: 61  CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 120
           CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS
Sbjct: 122 CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 181

Query: 121 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 180
           QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGIL
Sbjct: 182 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 241

Query: 181 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 240
           VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV
Sbjct: 242 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 301

Query: 241 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTA 300
           IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTA
Sbjct: 302 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTA 361

Query: 301 GGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 360
           GGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 362 GGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 421

Query: 361 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPITLVHFPQWGSMFLPPTKHVEKSTEE 420
           GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPITLVHFPQWGSMFLPPTKHVEKSTEE
Sbjct: 422 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPITLVHFPQWGSMFLPPTKHVEKSTEE 481

Query: 421 FYYGSEWTEDEKKK 435
           FYYGSEWTEDEKKK
Sbjct: 482 FYYGSEWTEDEKKK 495

BLAST of MELO3C000140 vs. NCBI nr
Match: NP_001274401.1 (high affinity nitrate transporter 2.4-like [Cucumis sativus] >AAS93686.3 high-affinity nitrate transporter [Cucumis sativus])

HSP 1 Score: 859.8 bits (2220), Expect = 1.0e-245
Identity = 430/434 (99.08%), Postives = 432/434 (99.54%), Query Frame = 0

Query: 1   MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 60
           MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV
Sbjct: 62  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 121

Query: 61  CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 120
           CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS
Sbjct: 122 CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 181

Query: 121 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 180
           QIIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFFIPGFLHVLMGIL
Sbjct: 182 QIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 241

Query: 181 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 240
           VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV
Sbjct: 242 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 301

Query: 241 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTA 300
           IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 
Sbjct: 302 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTF 361

Query: 301 GGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 360
           GGVFCMWLGKATKLPIAI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 362 GGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 421

Query: 361 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPITLVHFPQWGSMFLPPTKHVEKSTEE 420
           GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLP+TLVHFPQWGSMFLPPTKHVEKSTEE
Sbjct: 422 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 481

Query: 421 FYYGSEWTEDEKKK 435
           FYYGSEWTEDEKKK
Sbjct: 482 FYYGSEWTEDEKKK 495

BLAST of MELO3C000140 vs. NCBI nr
Match: XP_031736393.1 (high affinity nitrate transporter 2.4-like isoform X1 [Cucumis sativus] >AZP53714.1 high affinity nitrate transporter 2.1 [Cucumis sativus] >KAE8652752.1 hypothetical protein Csa_014056 [Cucumis sativus])

HSP 1 Score: 859.8 bits (2220), Expect = 1.0e-245
Identity = 430/434 (99.08%), Postives = 432/434 (99.54%), Query Frame = 0

Query: 1   MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 60
           MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV
Sbjct: 62  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 121

Query: 61  CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 120
           CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS
Sbjct: 122 CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 181

Query: 121 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 180
           QIIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFFIPGFLHVLMGIL
Sbjct: 182 QIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 241

Query: 181 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 240
           VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV
Sbjct: 242 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 301

Query: 241 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTA 300
           IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 
Sbjct: 302 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTF 361

Query: 301 GGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 360
           GGVFCMWLGKATKLPIAI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 362 GGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 421

Query: 361 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPITLVHFPQWGSMFLPPTKHVEKSTEE 420
           GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLP+TLVHFPQWGSMFLPPTKHVEKSTEE
Sbjct: 422 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 481

Query: 421 FYYGSEWTEDEKKK 435
           FYYGSEWTEDEKKK
Sbjct: 482 FYYGSEWTEDEKKK 495

BLAST of MELO3C000140 vs. NCBI nr
Match: AGO64298.1 (high-affinity nitrate transporter NRT2.2 [Cucumis sativus])

HSP 1 Score: 857.4 bits (2214), Expect = 5.2e-245
Identity = 428/434 (98.62%), Postives = 432/434 (99.54%), Query Frame = 0

Query: 1   MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 60
           MRTFHLSW+SFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV
Sbjct: 62  MRTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 121

Query: 61  CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 120
           CDLLGPRYGCAFLIMLSAPTVFCMSFVS+AAGYIAVRFMIGFSLATFVSCQYWMSTMFNS
Sbjct: 122 CDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 181

Query: 121 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 180
           QIIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFFIPGFLHVLMGIL
Sbjct: 182 QIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 241

Query: 181 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 240
           VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV
Sbjct: 242 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 301

Query: 241 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTA 300
           IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 
Sbjct: 302 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTF 361

Query: 301 GGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 360
           GGVFCMWLGKATKLPIAI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 362 GGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 421

Query: 361 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPITLVHFPQWGSMFLPPTKHVEKSTEE 420
           GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLP+TLVHFPQWGSMFLPPTKHVEKSTEE
Sbjct: 422 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 481

Query: 421 FYYGSEWTEDEKKK 435
           FYYGSEWTEDEKKK
Sbjct: 482 FYYGSEWTEDEKKK 495

BLAST of MELO3C000140 vs. ExPASy TrEMBL
Match: A0A5D3DCT6 (High affinity nitrate transporter 2.4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold609G00320 PE=4 SV=1)

HSP 1 Score: 865.5 bits (2235), Expect = 9.2e-248
Identity = 434/434 (100.00%), Postives = 434/434 (100.00%), Query Frame = 0

Query: 1   MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 60
           MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV
Sbjct: 62  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 121

Query: 61  CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 120
           CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS
Sbjct: 122 CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 181

Query: 121 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 180
           QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGIL
Sbjct: 182 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 241

Query: 181 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 240
           VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV
Sbjct: 242 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 301

Query: 241 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTA 300
           IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTA
Sbjct: 302 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTA 361

Query: 301 GGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 360
           GGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 362 GGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 421

Query: 361 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPITLVHFPQWGSMFLPPTKHVEKSTEE 420
           GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPITLVHFPQWGSMFLPPTKHVEKSTEE
Sbjct: 422 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPITLVHFPQWGSMFLPPTKHVEKSTEE 481

Query: 421 FYYGSEWTEDEKKK 435
           FYYGSEWTEDEKKK
Sbjct: 482 FYYGSEWTEDEKKK 495

BLAST of MELO3C000140 vs. ExPASy TrEMBL
Match: A0A1S3CTW4 (high affinity nitrate transporter 2.4-like OS=Cucumis melo OX=3656 GN=LOC103504472 PE=4 SV=1)

HSP 1 Score: 865.5 bits (2235), Expect = 9.2e-248
Identity = 434/434 (100.00%), Postives = 434/434 (100.00%), Query Frame = 0

Query: 1   MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 60
           MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV
Sbjct: 1   MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 60

Query: 61  CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 120
           CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS
Sbjct: 61  CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 120

Query: 121 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 180
           QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGIL
Sbjct: 121 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 180

Query: 181 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 240
           VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV
Sbjct: 181 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 240

Query: 241 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTA 300
           IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTA
Sbjct: 241 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTA 300

Query: 301 GGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 360
           GGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 301 GGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 360

Query: 361 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPITLVHFPQWGSMFLPPTKHVEKSTEE 420
           GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPITLVHFPQWGSMFLPPTKHVEKSTEE
Sbjct: 361 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPITLVHFPQWGSMFLPPTKHVEKSTEE 420

Query: 421 FYYGSEWTEDEKKK 435
           FYYGSEWTEDEKKK
Sbjct: 421 FYYGSEWTEDEKKK 434

BLAST of MELO3C000140 vs. ExPASy TrEMBL
Match: Q6PRP8 (High-affinity nitrate transporter OS=Cucumis sativus OX=3659 GN=NRT2 PE=2 SV=3)

HSP 1 Score: 859.8 bits (2220), Expect = 5.0e-246
Identity = 430/434 (99.08%), Postives = 432/434 (99.54%), Query Frame = 0

Query: 1   MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 60
           MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV
Sbjct: 62  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 121

Query: 61  CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 120
           CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS
Sbjct: 122 CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 181

Query: 121 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 180
           QIIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFFIPGFLHVLMGIL
Sbjct: 182 QIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 241

Query: 181 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 240
           VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV
Sbjct: 242 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 301

Query: 241 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTA 300
           IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 
Sbjct: 302 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTF 361

Query: 301 GGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 360
           GGVFCMWLGKATKLPIAI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 362 GGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 421

Query: 361 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPITLVHFPQWGSMFLPPTKHVEKSTEE 420
           GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLP+TLVHFPQWGSMFLPPTKHVEKSTEE
Sbjct: 422 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 481

Query: 421 FYYGSEWTEDEKKK 435
           FYYGSEWTEDEKKK
Sbjct: 482 FYYGSEWTEDEKKK 495

BLAST of MELO3C000140 vs. ExPASy TrEMBL
Match: A0A3Q9CZ42 (High affinity nitrate transporter 2.1 OS=Cucumis sativus OX=3659 GN=NRT2.1 PE=2 SV=1)

HSP 1 Score: 859.8 bits (2220), Expect = 5.0e-246
Identity = 430/434 (99.08%), Postives = 432/434 (99.54%), Query Frame = 0

Query: 1   MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 60
           MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV
Sbjct: 62  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 121

Query: 61  CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 120
           CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS
Sbjct: 122 CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 181

Query: 121 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 180
           QIIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFFIPGFLHVLMGIL
Sbjct: 182 QIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 241

Query: 181 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 240
           VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV
Sbjct: 242 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 301

Query: 241 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTA 300
           IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 
Sbjct: 302 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTF 361

Query: 301 GGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 360
           GGVFCMWLGKATKLPIAI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 362 GGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 421

Query: 361 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPITLVHFPQWGSMFLPPTKHVEKSTEE 420
           GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLP+TLVHFPQWGSMFLPPTKHVEKSTEE
Sbjct: 422 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 481

Query: 421 FYYGSEWTEDEKKK 435
           FYYGSEWTEDEKKK
Sbjct: 482 FYYGSEWTEDEKKK 495

BLAST of MELO3C000140 vs. ExPASy TrEMBL
Match: S4VM42 (High-affinity nitrate transporter NRT2.2 OS=Cucumis sativus OX=3659 GN=NRT2.2 PE=2 SV=1)

HSP 1 Score: 857.4 bits (2214), Expect = 2.5e-245
Identity = 428/434 (98.62%), Postives = 432/434 (99.54%), Query Frame = 0

Query: 1   MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 60
           MRTFHLSW+SFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV
Sbjct: 62  MRTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 121

Query: 61  CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 120
           CDLLGPRYGCAFLIMLSAPTVFCMSFVS+AAGYIAVRFMIGFSLATFVSCQYWMSTMFNS
Sbjct: 122 CDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 181

Query: 121 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 180
           QIIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFFIPGFLHVLMGIL
Sbjct: 182 QIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 241

Query: 181 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 240
           VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV
Sbjct: 242 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 301

Query: 241 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTA 300
           IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 
Sbjct: 302 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTF 361

Query: 301 GGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 360
           GGVFCMWLGKATKLPIAI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 362 GGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 421

Query: 361 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPITLVHFPQWGSMFLPPTKHVEKSTEE 420
           GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLP+TLVHFPQWGSMFLPPTKHVEKSTEE
Sbjct: 422 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 481

Query: 421 FYYGSEWTEDEKKK 435
           FYYGSEWTEDEKKK
Sbjct: 482 FYYGSEWTEDEKKK 495

BLAST of MELO3C000140 vs. TAIR 10
Match: AT5G60770.1 (nitrate transporter 2.4 )

HSP 1 Score: 732.3 bits (1889), Expect = 2.3e-211
Identity = 353/434 (81.34%), Postives = 395/434 (91.01%), Query Frame = 0

Query: 1   MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 60
           MRTFHL+WISFFTCF+STFAAAPLVPIIRDNLNLT+ D+GNAGV SVSGSIFSRLVMGAV
Sbjct: 62  MRTFHLAWISFFTCFISTFAAAPLVPIIRDNLNLTRQDVGNAGVASVSGSIFSRLVMGAV 121

Query: 61  CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 120
           CDLLGPRYGCAFL+MLSAPTVF MSFV  A GYI VRFMIGF LATFVSCQYWMSTMFN 
Sbjct: 122 CDLLGPRYGCAFLVMLSAPTVFSMSFVGGAGGYITVRFMIGFCLATFVSCQYWMSTMFNG 181

Query: 121 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 180
           QIIGLVNGTAAGWGNMGGG TQL+MP++Y++I+R G+T FTAWR+AFF+PG++H++MGIL
Sbjct: 182 QIIGLVNGTAAGWGNMGGGVTQLLMPMVYEIIRRLGSTSFTAWRMAFFVPGWMHIIMGIL 241

Query: 181 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 240
           VLTLGQDLPDGN ++L+KKG V KDKFS VLWYA+TNYRTW+FVLLYGYSMGVEL+TDNV
Sbjct: 242 VLTLGQDLPDGNRSTLEKKGAVTKDKFSKVLWYAITNYRTWVFVLLYGYSMGVELTTDNV 301

Query: 241 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTA 300
           IAEYF+DRF LKLHTAGIIAA+FGMAN  ARP GG+ASD+AAR FGMRGRLWTLWI+QT 
Sbjct: 302 IAEYFFDRFHLKLHTAGIIAASFGMANFFARPIGGWASDIAARRFGMRGRLWTLWIIQTL 361

Query: 301 GGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 360
           GG FC+WLG+AT LP A+V MILFS+GAQAACGATF IIPFISRRSLGIISGLTGAGGNF
Sbjct: 362 GGFFCLWLGRATTLPTAVVFMILFSLGAQAACGATFAIIPFISRRSLGIISGLTGAGGNF 421

Query: 361 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPITLVHFPQWGSMFLPPTKHVEKSTEE 420
           GSGLTQLVFFST+ +ST+ GL  MG+MI+ CTLP+TLVHFPQWGSMFLP T+   KSTEE
Sbjct: 422 GSGLTQLVFFSTSTFSTEQGLTWMGVMIMACTLPVTLVHFPQWGSMFLPSTEDEVKSTEE 481

Query: 421 FYYGSEWTEDEKKK 435
           +YY  EWTE EK+K
Sbjct: 482 YYYMKEWTETEKRK 495

BLAST of MELO3C000140 vs. TAIR 10
Match: AT1G08090.1 (nitrate transporter 2:1 )

HSP 1 Score: 729.6 bits (1882), Expect = 1.5e-210
Identity = 354/434 (81.57%), Postives = 394/434 (90.78%), Query Frame = 0

Query: 1   MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 60
           MRTFHLSWISF TCFVSTFAAAPLVPIIR+NLNLTK DIGNAGV SVSGSIFSRLVMGAV
Sbjct: 62  MRTFHLSWISFSTCFVSTFAAAPLVPIIRENLNLTKQDIGNAGVASVSGSIFSRLVMGAV 121

Query: 61  CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 120
           CDLLGPRYGCAFL+MLSAPTVF MSFVS+AAG+I VRFMIGF LATFVSCQYWMSTMFNS
Sbjct: 122 CDLLGPRYGCAFLVMLSAPTVFSMSFVSDAAGFITVRFMIGFCLATFVSCQYWMSTMFNS 181

Query: 121 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 180
           QIIGLVNGTAAGWGNMGGG TQL+MP++Y++I+R G+T FTAWRIAFF+PG+LH++MGIL
Sbjct: 182 QIIGLVNGTAAGWGNMGGGITQLLMPIVYEIIRRCGSTAFTAWRIAFFVPGWLHIIMGIL 241

Query: 181 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 240
           VL LGQDLPDGN A+L+K G VAKDKF  +LWYAVTNYRTWIFVLLYGYSMGVELSTDNV
Sbjct: 242 VLNLGQDLPDGNRATLEKAGEVAKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDNV 301

Query: 241 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTA 300
           IAEYF+DRF LKLHTAG+IAA FGMAN  ARP GGYASD AA+YFGMRGRLWTLWI+QTA
Sbjct: 302 IAEYFFDRFHLKLHTAGLIAACFGMANFFARPAGGYASDFAAKYFGMRGRLWTLWIIQTA 361

Query: 301 GGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 360
           GG+FC+WLG+A  L  A+VAM+LFS+GAQAACGATF I+PF+SRR+LGIISGLTGAGGNF
Sbjct: 362 GGLFCVWLGRANTLVTAVVAMVLFSMGAQAACGATFAIVPFVSRRALGIISGLTGAGGNF 421

Query: 361 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPITLVHFPQWGSMFLPPTKHVEKSTEE 420
           GSGLTQL+FFST+ ++T+ GL  MG+MIV CTLP+TLVHFPQWGSMFLPP+    K TE 
Sbjct: 422 GSGLTQLLFFSTSHFTTEQGLTWMGVMIVACTLPVTLVHFPQWGSMFLPPSTDPVKGTEA 481

Query: 421 FYYGSEWTEDEKKK 435
            YYGSEW E EK+K
Sbjct: 482 HYYGSEWNEQEKQK 495

BLAST of MELO3C000140 vs. TAIR 10
Match: AT1G08100.1 (nitrate transporter 2.2 )

HSP 1 Score: 706.8 bits (1823), Expect = 1.1e-203
Identity = 341/433 (78.75%), Postives = 394/433 (90.99%), Query Frame = 0

Query: 1   MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 60
           MRTFHLSWISF TCFVSTFAAAPL+PIIR+NLNLTK DIGNAGV SVSGSIFSRLVMGAV
Sbjct: 60  MRTFHLSWISFSTCFVSTFAAAPLIPIIRENLNLTKHDIGNAGVASVSGSIFSRLVMGAV 119

Query: 61  CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 120
           CDLLGPRYGCAFL+MLSAPTVF MSFVS+AAG+I VRFMIGF LATFVSCQYWMSTMFNS
Sbjct: 120 CDLLGPRYGCAFLVMLSAPTVFSMSFVSDAAGFITVRFMIGFCLATFVSCQYWMSTMFNS 179

Query: 121 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 180
           QIIGLVNGTAAGWGNMGGG TQL+MP++Y++I+R G+T FTAWRIAFF+PG+LH++MGIL
Sbjct: 180 QIIGLVNGTAAGWGNMGGGITQLLMPIVYEIIRRCGSTAFTAWRIAFFVPGWLHIIMGIL 239

Query: 181 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 240
           VLTLGQDLP GN A+++K G VAKDKF  +LWYAVTNYRTWIFVLLYGYSMGVELSTDNV
Sbjct: 240 VLTLGQDLPGGNRAAMEKAGEVAKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDNV 299

Query: 241 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTA 300
           IAEYF+DRF LKLHTAGIIAA FGMAN  ARP GG+ASD+AA+ FGMRGRLWTLWI+QT+
Sbjct: 300 IAEYFFDRFHLKLHTAGIIAACFGMANFFARPAGGWASDIAAKRFGMRGRLWTLWIIQTS 359

Query: 301 GGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 360
           GG+FC+WLG+A  L  A+V+M+LFS+GAQAACGATF I+PF+SRR+LGIISGLTGAGGNF
Sbjct: 360 GGLFCVWLGRANTLVTAVVSMVLFSLGAQAACGATFAIVPFVSRRALGIISGLTGAGGNF 419

Query: 361 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPITLVHFPQWGSMFLPPTKHVEKSTEE 420
           GSGLTQLVFFST++++T+ GL  MG+MIV CTLP+TL+HFPQWGSMF PP+     +TE 
Sbjct: 420 GSGLTQLVFFSTSRFTTEEGLTWMGVMIVACTLPVTLIHFPQWGSMFFPPSNDSVDATEH 479

Query: 421 FYYGSEWTEDEKK 434
           +Y G E++++E++
Sbjct: 480 YYVG-EYSKEEQQ 491

BLAST of MELO3C000140 vs. TAIR 10
Match: AT5G60780.1 (nitrate transporter 2.3 )

HSP 1 Score: 674.9 bits (1740), Expect = 4.4e-194
Identity = 324/434 (74.65%), Postives = 377/434 (86.87%), Query Frame = 0

Query: 1   MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 60
           MR FHL W+SFFTCF+STFAAAPLVPIIRDNL+LTK DIGNAGV SVSG+IFSRL MGAV
Sbjct: 69  MRAFHLGWLSFFTCFISTFAAAPLVPIIRDNLDLTKTDIGNAGVASVSGAIFSRLAMGAV 128

Query: 61  CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 120
           CDLLG RYG AF +ML+APTVF MSFV   +GY+ VRFMIGF LATFVSCQYW S MFN 
Sbjct: 129 CDLLGARYGTAFSLMLTAPTVFSMSFVGGPSGYLGVRFMIGFCLATFVSCQYWTSVMFNG 188

Query: 121 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 180
           +IIGLVNG A GWG+MGGG TQL+MP+++ VI+ AGATPF AWRIAFF+PGFL V+MGIL
Sbjct: 189 KIIGLVNGCAGGWGDMGGGVTQLLMPMVFHVIKLAGATPFMAWRIAFFVPGFLQVVMGIL 248

Query: 181 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 240
           VL+LGQDLPDGNL++LQK G V+KDKFS V W+AV NYRTWI  +LYG SMG+EL+ +NV
Sbjct: 249 VLSLGQDLPDGNLSTLQKSGQVSKDKFSKVFWFAVKNYRTWILFVLYGSSMGIELTINNV 308

Query: 241 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTA 300
           I+ YFYDRF+LKL TAGI+AA+FGMAN +ARPFGGYASDVAAR FGMRGRLWTLWI QT 
Sbjct: 309 ISGYFYDRFNLKLQTAGIVAASFGMANFIARPFGGYASDVAARVFGMRGRLWTLWIFQTV 368

Query: 301 GGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 360
           G +FC+WLG+A+ LPIAI+AM+LFSIG QAACGA FG+ PF+SRRSLG+ISGLTGAGGNF
Sbjct: 369 GALFCIWLGRASSLPIAILAMMLFSIGTQAACGALFGVAPFVSRRSLGLISGLTGAGGNF 428

Query: 361 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPITLVHFPQWGSMFLPPTKHVEKSTEE 420
           GSGLTQL+FFS+ ++ST  GL LMG+M V CTLP+  +HFPQWGSMFL P+   E+S EE
Sbjct: 429 GSGLTQLLFFSSARFSTAEGLSLMGVMAVLCTLPVAFIHFPQWGSMFLRPSTDGERSQEE 488

Query: 421 FYYGSEWTEDEKKK 435
           +YYGSEWTE+EK++
Sbjct: 489 YYYGSEWTENEKQQ 502

BLAST of MELO3C000140 vs. TAIR 10
Match: AT3G45060.1 (high affinity nitrate transporter 2.6 )

HSP 1 Score: 668.3 bits (1723), Expect = 4.1e-192
Identity = 321/432 (74.31%), Postives = 374/432 (86.57%), Query Frame = 0

Query: 1   MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 60
           MR FHL WISFFTCF+STFAAAPLVP+IRDNL+LTK DIGNAGV SVSG+IFSRL MGAV
Sbjct: 72  MRAFHLGWISFFTCFISTFAAAPLVPVIRDNLDLTKTDIGNAGVASVSGAIFSRLAMGAV 131

Query: 61  CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 120
           CDLLG RYG AF +ML+AP VF MSFV++A  Y+AVRFMIGF LATFVSCQYW S MF  
Sbjct: 132 CDLLGARYGTAFSLMLTAPAVFSMSFVADAGSYLAVRFMIGFCLATFVSCQYWTSVMFTG 191

Query: 121 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 180
           +IIGLVNG A GWG+MGGG TQL+MP+++ VI+  GATPFTAWR AFFIPG L ++MGIL
Sbjct: 192 KIIGLVNGCAGGWGDMGGGVTQLLMPMVFHVIKLTGATPFTAWRFAFFIPGILQIVMGIL 251

Query: 181 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 240
           VLTLGQDLPDGNL++LQK G V+KDKFS V W+AV NYRTWI  +LYG+SMGVEL+ +NV
Sbjct: 252 VLTLGQDLPDGNLSTLQKSGQVSKDKFSKVFWFAVKNYRTWILFMLYGFSMGVELTINNV 311

Query: 241 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTA 300
           I+ YFYDRF+L LHTAGIIAA+FGMAN  ARPFGGYASDVAAR FGMRGRLW LWILQT 
Sbjct: 312 ISGYFYDRFNLTLHTAGIIAASFGMANFFARPFGGYASDVAARLFGMRGRLWILWILQTV 371

Query: 301 GGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 360
           G +FC+WLG+A+ LPIAI+AM+LFS+G QAACGA FG+ PF+SRRSLG+ISGLTGAGGNF
Sbjct: 372 GALFCIWLGRASSLPIAILAMMLFSMGTQAACGALFGVAPFVSRRSLGLISGLTGAGGNF 431

Query: 361 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPITLVHFPQWGSMFLPPTKHVEKSTEE 420
           GSG+TQL+FFS++++ST  GL LMG+M V C+LP+  +HFPQWGSMFL P++  EKS EE
Sbjct: 432 GSGVTQLLFFSSSRFSTAEGLSLMGVMAVVCSLPVAFIHFPQWGSMFLRPSQDGEKSKEE 491

Query: 421 FYYGSEWTEDEK 433
            YYG+EWTE+EK
Sbjct: 492 HYYGAEWTEEEK 503

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FJH83.3e-21081.34High affinity nitrate transporter 2.4 OS=Arabidopsis thaliana OX=3702 GN=NRT2.4 ... [more]
O828112.1e-20981.57High-affinity nitrate transporter 2.1 OS=Arabidopsis thaliana OX=3702 GN=NRT2.1 ... [more]
P0DKG92.4e-20580.41High-affinity nitrate transporter 2.1 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
P0DKH02.4e-20580.41High-affinity nitrate transporter 2.2 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q9LMZ91.5e-20278.75High-affinity nitrate transporter 2.2 OS=Arabidopsis thaliana OX=3702 GN=NRT2.2 ... [more]
Match NameE-valueIdentityDescription
XP_008467040.11.9e-247100.00PREDICTED: high affinity nitrate transporter 2.4-like, partial [Cucumis melo][more]
TYK21386.11.9e-247100.00high affinity nitrate transporter 2.4-like [Cucumis melo var. makuwa][more]
NP_001274401.11.0e-24599.08high affinity nitrate transporter 2.4-like [Cucumis sativus] >AAS93686.3 high-af... [more]
XP_031736393.11.0e-24599.08high affinity nitrate transporter 2.4-like isoform X1 [Cucumis sativus] >AZP5371... [more]
AGO64298.15.2e-24598.62high-affinity nitrate transporter NRT2.2 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A5D3DCT69.2e-248100.00High affinity nitrate transporter 2.4-like OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3CTW49.2e-248100.00high affinity nitrate transporter 2.4-like OS=Cucumis melo OX=3656 GN=LOC1035044... [more]
Q6PRP85.0e-24699.08High-affinity nitrate transporter OS=Cucumis sativus OX=3659 GN=NRT2 PE=2 SV=3[more]
A0A3Q9CZ425.0e-24699.08High affinity nitrate transporter 2.1 OS=Cucumis sativus OX=3659 GN=NRT2.1 PE=2 ... [more]
S4VM422.5e-24598.62High-affinity nitrate transporter NRT2.2 OS=Cucumis sativus OX=3659 GN=NRT2.2 PE... [more]
Match NameE-valueIdentityDescription
AT5G60770.12.3e-21181.34nitrate transporter 2.4 [more]
AT1G08090.11.5e-21081.57nitrate transporter 2:1 [more]
AT1G08100.11.1e-20378.75nitrate transporter 2.2 [more]
AT5G60780.14.4e-19474.65nitrate transporter 2.3 [more]
AT3G45060.14.1e-19274.31high affinity nitrate transporter 2.6 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 1..193
e-value: 9.5E-21
score: 76.1
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 203..405
e-value: 4.7E-27
score: 96.7
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 2..400
IPR011701Major facilitator superfamilyPFAMPF07690MFS_1coord: 8..364
e-value: 3.5E-20
score: 72.2
IPR044772Nitrate transporterPANTHERPTHR23515HIGH-AFFINITY NITRATE TRANSPORTER 2.3coord: 1..434
IPR044772Nitrate transporterCDDcd17341MFS_NRT2_likecoord: 4..397
e-value: 3.43956E-129
score: 376.207
NoneNo IPR availablePANTHERPTHR23515:SF16HIGH-AFFINITY NITRATE TRANSPORTER 2.1-LIKEcoord: 1..434
IPR020846Major facilitator superfamily domainPROSITEPS50850MFScoord: 1..397
score: 9.293217

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C000140.1MELO3C000140.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
molecular_function GO:0015112 nitrate transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity