MC11g_new0170 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC11g_new0170
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	cytochrome P450 CYP72A219-like
Location	MC11: 6614508 .. 6617458 (-)
RNA-Seq Expression	MC11g_new0170
Synteny	MC11g_new0170

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GCCTGCTCGATCTACCTCAACTCACGCTTAATTGTCATTTTTGAAAATGCGGTTCCATGTCGGTTCAACTGTAAAACCGAACCAAATTGAACGTACGGAACACTGGGAGTCGGCAAGGACATATATTAGTTGCAATTTCCTCTGGTAGAGATTGAAATCGCTTCCTCTCTCTCTCTCTTTCTCTCTTGTCTATAGATGGAAACGTGGGTTGCAGCTACGATTTCAATTTGGATAATAGTTGGGGTATGGTTACTGGTATGGGGATGGAGAGTTGTGGACTGGATCTGGGTGAGGCCAAAGAAATTAGAGAAGTACTTGAGAGATCAAGGCCTCGCCGGAAATTCTTACCGGATTCTCGTCGGAGACTGGAAGGACATGTCGGCGATGCAGAAACAGGCCATGTCCAAGCCCATGAACTTCTCCAATGATATTGCTCCACGCATCGTCCCTTTCCTTCATCGAACCATCCAAATTTACGGTAAACAAACAAACGCATACATATCATATCATATATCATGCCCCAAAATTGAAGCAAAGTTTGTGAAGAAAAATTAGTCTTTTTTTCTTAGATCAAGAACATCTGGTGGTGATTATATATAAATGTGTTTTTTTTTTTTTTGGTTAACTAAACTGAGGAATAGTACTAATCCGTGTATGCACTGGCTCATTATATTTACCTATCTCATCACAAAGTTGATGGTTGGATCTCAAACACGAACATCAACGCCGTTAAAGATCTCACCTTGAAAGAAAAATGAACTTACAATAATTTATAAGTTGGTTTTTGGATGGAATCCATAAATATTAATATGGTATTAGAATCCAAAACGTTCAAACCAACAATCGATTTATTATTAGACTCAGAACAACAAAACTTTATTGTAAAATATGCAAATTAATGGTAGGGAATTGCAGGAAAAAAATTCATTTACGTGGAACGGCCCCATTCCAAGTGTGCTGACGATGGAGCCAGAACAAGTTAAAACAGCATTTATGCAAGTGGATGATTTCCGAAAGCCAAAGATGGACCATCTTATTAAGTTGATTGCTGATGGACTTGTAAACCATGAAGGACCAAAATGGGAGAAACACAGAAAAATAATCAACCCTGCATTTCATTTGGAAAAGTTGAAGGTATACCTCCCTCTTTTGATTTTGATTTTATGTTTTACTTATTTGTATCCTTCAATTATTGGCTTATAGGATATGGTACCAGCATTCTATCAATGTTGTGACGAGATGGTTAATAAATGGGAGAGAATGGTCTCTAAAGAGGGACAGTGCGAGTTGGATGTAATACCTTATCTACAAACTATGACTGTCGATGCCATTTCTCGAACAACATTTGGGAGTAGCTATGAAAAAGGAAAAAAGATCTTCGAACTTCAAAAACAACTAAATTTTTCGGTGATTAAATATTTGTCAATGATGACCAATCCAATATGGAGGTAAAAACAAACATTTCTAACTAAAAGTTTAGCTTGTCGTAAAGTGTTTTTTTTTTCCTTCTTTTCCTTTTTTTGTTTATAATTTGAAGGTTTTAGGTCTATACAAGCACCACATCCACCAATGGTAAATTTAACTTTCACTTTGTAAAGATTTATTATTACTATTTTATTACTTATACAAATTACATGATTCTCAAGTACCTCAATTACCATATCTGAGGTCGATGGTTCAATTTCTCACCTCACAACGGTTGAATTCAAAAAGATTAAAAAAAAACTGTATTAATAGAGAGCTAATATTTTTATATTTCATGAATCATAGAGCTTAGTCACATCAAAATATCTTGTTGGTATATATATATTTGTTCTATTTACTCCATGTTCTTCATTTTGGTTCAAAACATAATCCATTACGAAAAAAGTTTCCCCTCAAAATTATTTGATGTGAGCATTAATTGTGTGCAGGTTTTTACCCACCAAGTCAAAAAATAAAATGAAAAAGATGGGTAAAGAGATTAAAAGTTTGATATTGGGTATTATAAATGAAAGACAGAAGGCTATGGAGGCAGGTGAAGCTGCATACAGTGATCTATTAGGCATACTGATGGAATCTAATCTCAACGAAATTCGACAACATGGAAATAACAAAAATGTTGGAATGAGTATAGAAGATGTTATCGAAGAATGCAAGCTTTTCTATCTTGCTGGCCAAGAAACTACAGCTACATTACTAATTTGGACTATGATTTTATTGAGTTCATACTCAGAATGGCAAGAAAGAGCAAGAGCAGAGGTTCGAGAAGTCTTTGATAACAGAAAGCCAGATTATAACGGTTTAAGTCGTCTAAAAATTGTAAGTGTGTGATTTCATTTTTCTGTATTTACCCTTGTGCTCTATCTTCTCGTGCAAATAAACTTAAGGATTACAATTCTTGGAATTGCACAGGTAACTATGATTTTAAACGAAGTTCTTAGGTTATACCCACCGGTAAGTGTGCTTGATCGTGTCGTTGAAAAGGAAATGAAGCTTGGAAATTTGTCTTTACCAGCGGGAGTGATATTGAGGTTACCAATCATTTTCATCCATCATGATCGTGAGCTATGGGGTGAAGATGCACTTGAATTCAATCCACAAAGATTTTTTGATGGAGTTTCTAAAGCAACAAAAAATCAAACCATTTTTATCCCATTTGGTTTGGGTCCTCGAACATGCCTCGGACAAAACTTCGCCATGATTGAGGCAAAAATGGCACTGTCAACAATTCTACAGCAGTTCTCATTCCAGCTTTCTCCATCATATACTCATGCTCCCTTCGTAACTATAACAACCCAACCTCAACATGGAGCTCATATCATCCTCCACAAACTCAACTAAGTTACTTAAACAAATCAAAAGTTGTGAAATTAAGTACTTTGTTGTATATAATTTCAGTTATTTTTAGTAAAAGATATATACACAAATGAACATAATGCTCCGGGATTATTTTGTTCACGAGCTTGCTT

mRNA sequence

ATGATATTGCTCCACGCATCGTCCCTTTCCTTCATCGAACCATCCAAATTTACGGAAAAAAATTCATTTACGTGGAACGGCCCCATTCCAAGTGTGCTGACGATGGAGCCAGAACAAGTTAAAACAGCATTTATGCAAGTGGATGATTTCCGAAAGCCAAAGATGGACCATCTTATTAAGTTGATTGCTGATGGACTTGTAAACCATGAAGGACCAAAATGGGAGAAACACAGAAAAATAATCAACCCTGCATTTCATTTGGAAAAGTTGAAGGATATGGTACCAGCATTCTATCAATGTTGTGACGAGATGGTTAATAAATGGGAGAGAATGGTCTCTAAAGAGGGACAGTGCGAGTTGGATGTAATACCTTATCTACAAACTATGACTGTCGATGCCATTTCTCGAACAACATTTGGGAGTAGCTATGAAAAAGGAAAAAAGATCTTCGAACTTCAAAAACAACTAAATTTTTCGGTGATTAAATATTTGTCAATGATGACCAATCCAATATGGAGGTTTTTACCCACCAAGTCAAAAAATAAAATGAAAAAGATGGGTAAAGAGATTAAAAGTTTGATATTGGGTATTATAAATGAAAGACAGAAGGCTATGGAGGCAGGTGAAGCTGCATACAGTGATCTATTAGGCATACTGATGGAATCTAATCTCAACGAAATTCGACAACATGGAAATAACAAAAATGTTGGAATGAGTATAGAAGATGTTATCGAAGAATGCAAGCTTTTCTATCTTGCTGGCCAAGAAACTACAGCTACATTACTAATTTGGACTATGATTTTATTGAGTTCATACTCAGAATGGCAAGAAAGAGCAAGAGCAGAGGTTCGAGAAGTCTTTGATAACAGAAAGCCAGATTATAACGGTTTAAGTCGTCTAAAAATTGTAACTATGATTTTAAACGAAGTTCTTAGGTTATACCCACCGGTAAGTGTGCTTGATCGTGTCGTTGAAAAGGAAATGAAGCTTGGAAATTTGTCTTTACCAGCGGGAGTGATATTGAGGTTACCAATCATTTTCATCCATCATGATCGTGAGCTATGGGGTGAAGATGCACTTGAATTCAATCCACAAAGATTTTTTGATGGAGTTTCTAAAGCAACAAAAAATCAAACCATTTTTATCCCATTTGGTTTGGGTCCTCGAACATGCCTCGGACAAAACTTCGCCATGATTGAGGCAAAAATGGCACTGTCAACAATTCTACAGCAGTTCTCATTCCAGCTTTCTCCATCATATACTCATGCTCCCTTCGTAACTATAACAACCCAACCTCAACATGGAGCTCATATCATCCTCCACAAACTCAACTAA

Coding sequence (CDS)

ATGATATTGCTCCACGCATCGTCCCTTTCCTTCATCGAACCATCCAAATTTACGGAAAAAAATTCATTTACGTGGAACGGCCCCATTCCAAGTGTGCTGACGATGGAGCCAGAACAAGTTAAAACAGCATTTATGCAAGTGGATGATTTCCGAAAGCCAAAGATGGACCATCTTATTAAGTTGATTGCTGATGGACTTGTAAACCATGAAGGACCAAAATGGGAGAAACACAGAAAAATAATCAACCCTGCATTTCATTTGGAAAAGTTGAAGGATATGGTACCAGCATTCTATCAATGTTGTGACGAGATGGTTAATAAATGGGAGAGAATGGTCTCTAAAGAGGGACAGTGCGAGTTGGATGTAATACCTTATCTACAAACTATGACTGTCGATGCCATTTCTCGAACAACATTTGGGAGTAGCTATGAAAAAGGAAAAAAGATCTTCGAACTTCAAAAACAACTAAATTTTTCGGTGATTAAATATTTGTCAATGATGACCAATCCAATATGGAGGTTTTTACCCACCAAGTCAAAAAATAAAATGAAAAAGATGGGTAAAGAGATTAAAAGTTTGATATTGGGTATTATAAATGAAAGACAGAAGGCTATGGAGGCAGGTGAAGCTGCATACAGTGATCTATTAGGCATACTGATGGAATCTAATCTCAACGAAATTCGACAACATGGAAATAACAAAAATGTTGGAATGAGTATAGAAGATGTTATCGAAGAATGCAAGCTTTTCTATCTTGCTGGCCAAGAAACTACAGCTACATTACTAATTTGGACTATGATTTTATTGAGTTCATACTCAGAATGGCAAGAAAGAGCAAGAGCAGAGGTTCGAGAAGTCTTTGATAACAGAAAGCCAGATTATAACGGTTTAAGTCGTCTAAAAATTGTAACTATGATTTTAAACGAAGTTCTTAGGTTATACCCACCGGTAAGTGTGCTTGATCGTGTCGTTGAAAAGGAAATGAAGCTTGGAAATTTGTCTTTACCAGCGGGAGTGATATTGAGGTTACCAATCATTTTCATCCATCATGATCGTGAGCTATGGGGTGAAGATGCACTTGAATTCAATCCACAAAGATTTTTTGATGGAGTTTCTAAAGCAACAAAAAATCAAACCATTTTTATCCCATTTGGTTTGGGTCCTCGAACATGCCTCGGACAAAACTTCGCCATGATTGAGGCAAAAATGGCACTGTCAACAATTCTACAGCAGTTCTCATTCCAGCTTTCTCCATCATATACTCATGCTCCCTTCGTAACTATAACAACCCAACCTCAACATGGAGCTCATATCATCCTCCACAAACTCAACTAA

Protein sequence

MILLHASSLSFIEPSKFTEKNSFTWNGPIPSVLTMEPEQVKTAFMQVDDFRKPKMDHLIKLIADGLVNHEGPKWEKHRKIINPAFHLEKLKDMVPAFYQCCDEMVNKWERMVSKEGQCELDVIPYLQTMTVDAISRTTFGSSYEKGKKIFELQKQLNFSVIKYLSMMTNPIWRFLPTKSKNKMKKMGKEIKSLILGIINERQKAMEAGEAAYSDLLGILMESNLNEIRQHGNNKNVGMSIEDVIEECKLFYLAGQETTATLLIWTMILLSSYSEWQERARAEVREVFDNRKPDYNGLSRLKIVTMILNEVLRLYPPVSVLDRVVEKEMKLGNLSLPAGVILRLPIIFIHHDRELWGEDALEFNPQRFFDGVSKATKNQTIFIPFGLGPRTCLGQNFAMIEAKMALSTILQQFSFQLSPSYTHAPFVTITTQPQHGAHIILHKLN

Homology

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BLAST of MC11g_new0170 vs. ExPASy Swiss-Prot
Match: H2DH21 (Cytochrome P450 CYP72A219 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 2.0e-146
Identity = 250/425 (58.82%), Postives = 324/425 (76.24%), Query Frame = 0


Query: 20  KNSFTWNGPIPSVLTMEPEQVKTAFMQVDDFRKPKMDHLIKLIADGLVNHEGPKWEKHRK 79
           KNSF W GP P V  M P+ +K    +   F+KP+ + L KL+A G+ + EG +W KHRK
Sbjct: 94  KNSFLWLGPKPMVHIMNPDHIKDVLSKFYQFQKPRHNPLTKLLATGVADAEGDRWAKHRK 153

Query: 80  IINPAFHLEKLKDMVPAFYQCCDEMVNKWERMVSKEGQCELDVIPYLQTMTVDAISRTTF 139
           +INPAFHLEKLK+M+PA Y    E+V KWE MVS +GQ ELDV+PYL+T+T D ISRT F
Sbjct: 154 LINPAFHLEKLKNMLPAIYLSSSEIVTKWEEMVSTKGQFELDVLPYLETLTSDVISRTAF 213

Query: 140 GSSYEKGKKIFELQKQLNFSVIKYLSMMTNPIWRFLPTKSKNKMKKMGKEIKSLILGIIN 199
           GSSYE+G+KIF+LQ++    +I+    +  P  RFLPTK   +MK++ KE+K  +  IIN
Sbjct: 214 GSSYEEGRKIFQLQREQAELIIQASQTIYLPGMRFLPTKRNKRMKEIAKEVKIALKSIIN 273

Query: 200 ERQKAMEAGE-AAYSDLLGILMESNLNEIRQHGNNKNVGMSIEDVIEECKLFYLAGQETT 259
           +R KAMEAGE +++ DLLGIL+ESN  EI+QHGN  N G+++++VIEECKLF+ AGQETT
Sbjct: 274 KRLKAMEAGERSSHDDLLGILLESNSKEIKQHGNT-NFGLTVDEVIEECKLFFFAGQETT 333

Query: 260 ATLLIWTMILLSSYSEWQERARAEVREVFDNRKPDYNGLSRLKIVTMILNEVLRLYPPVS 319
           + LL+WTMILLS + +WQ+RA+ EV   F N KPD++GL+ LK+V MIL EVLRLYPP+ 
Sbjct: 334 SNLLVWTMILLSQHQDWQKRAKEEVLRTFGNNKPDFDGLNHLKVVNMILLEVLRLYPPIL 393

Query: 320 VLDRVVEKEMKLGNLSLPAGVILRLPIIFIHHDRELWGEDALEFNPQRFFDGVSKATKNQ 379
            LDR + +E+KLG +SLPAGVIL LPII +H+D+E+WG+DA EFNP+RF +GV KATK +
Sbjct: 394 SLDRTIYEEIKLGEISLPAGVILLLPIILLHYDQEIWGDDAKEFNPERFSEGVLKATKGR 453

Query: 380 TIFIPFGLGPRTCLGQNFAMIEAKMALSTILQQFSFQLSPSYTHAPFVTITTQPQHGAHI 439
             + PF  GPR C+GQNFAM+EAKMA++ ILQ+FSF LSPSY HAP   IT QPQ+GAH+
Sbjct: 454 VTYFPFSWGPRICIGQNFAMLEAKMAMAMILQRFSFVLSPSYAHAPHAIITLQPQYGAHL 513

Query: 440 ILHKL 444
           ILH L
Sbjct: 514 ILHSL 517

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BLAST of MC11g_new0170 vs. ExPASy Swiss-Prot
Match: A0A0S2IHL2 (Cytochrome P450 72A397 OS=Kalopanax septemlobus OX=228393 GN=CYP72A397 PE=1 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 1.0e-145
Identity = 249/424 (58.73%), Postives = 321/424 (75.71%), Query Frame = 0


Query: 20  KNSFTWNGPIPSVLTMEPEQVKTAFMQVDDFRKPKMDHLIKLIADGLVNHEGPKWEKHRK 79
           K+SF W GP P V  M+PE +K   ++   F KPK + L+KL ADGL NHEG  W KHRK
Sbjct: 98  KSSFIWIGPTPRVQIMDPEIIKEIMVKSYKFNKPKRNPLVKLFADGLANHEGELWAKHRK 157

Query: 80  IINPAFHLEKLKDMVPAFYQCCDEMVNKWERMVSKEGQCELDVIPYLQTMTVDAISRTTF 139
           ++NPAFHLE+LK M+PA Y  C EMV+KW++M+SK+G  ELDV P+LQ +T D IS T F
Sbjct: 158 LLNPAFHLERLKCMLPAMYFSCIEMVSKWDKMISKDGSRELDVWPFLQRLTSDVISHTAF 217

Query: 140 GSSYEKGKKIFELQKQLNFSVIKYLSMMTNPIWRFLPTKSKNKMKKMGKEIKSLILGIIN 199
           GSSYE+G  +FELQ +    V+K L  +  P W +LPTK   KMK++ ++ +S ++ IIN
Sbjct: 218 GSSYEEGNIVFELQTEQAELVMKTLQSVYIPGWSYLPTKRNRKMKEIDRKTQSCLMNIIN 277

Query: 200 ERQKAMEAGEAAYSDLLGILMESNLNEIRQHGNNKNVGMSIEDVIEECKLFYLAGQETTA 259
           ++ KAM+AGE +  D+LGIL+ESNL E    G  KNVGMSI++V+ ECK FY AGQETT+
Sbjct: 278 KKTKAMQAGEGSTDDILGILLESNLKEQLGQG-KKNVGMSIQEVMGECKQFYFAGQETTS 337

Query: 260 TLLIWTMILLSSYSEWQERARAEVREVFDNRKPDYNGLSRLKIVTMILNEVLRLYPPVSV 319
            LL+WTM+LLS +  WQ RAR EV + F N KPD++ L+ LKIVTMIL EVLRLYPPV  
Sbjct: 338 GLLVWTMVLLSIHPNWQARAREEVLQQFGNAKPDFDNLNHLKIVTMILYEVLRLYPPVDT 397

Query: 320 LDRVVEKEMKLGNLSLPAGVILRLPIIFIHHDRELWGEDALEFNPQRFFDGVSKATKNQT 379
           L R V++E  LG+++LPAGV + LPI+ +HHD+ +WG+DA EFNP+RF +GVSKATKNQ 
Sbjct: 398 LFRRVDQETTLGDITLPAGVQISLPIMILHHDQNIWGDDAKEFNPERFSEGVSKATKNQV 457

Query: 380 IFIPFGLGPRTCLGQNFAMIEAKMALSTILQQFSFQLSPSYTHAPFVTITTQPQHGAHII 439
           +F PFG GPR C+GQNFA++EAK+AL+ ILQ+FSF+LSPSYTHAP   +T QPQHGA++I
Sbjct: 458 VFFPFGWGPRICIGQNFALLEAKLALAIILQRFSFELSPSYTHAPTTVLTVQPQHGANLI 517

Query: 440 LHKL 444
           LHKL
Sbjct: 518 LHKL 520

[Close]

BLAST of MC11g_new0170 vs. ExPASy Swiss-Prot
Match: A0A481NR20 (Cytochrome P450 72A552 OS=Barbarea vulgaris OX=50459 GN=CYP72A552 PE=1 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 2.7e-143
Identity = 246/425 (57.88%), Postives = 321/425 (75.53%), Query Frame = 0


Query: 20  KNSFTWNGPIPSVLTMEPEQVKTAFMQVDDFRKPKMDHLIKLIADGLVNHEGPKWEKHRK 79
           +  FTW GPIP++  M+PE +K  F +V DF+K ++  L +LIA GLV ++G KW KHRK
Sbjct: 94  RTHFTWLGPIPAITIMDPELIKEVFNRVYDFQKARLFPLARLIATGLVRYDGDKWAKHRK 153

Query: 80  IINPAFHLEKLKDMVPAFYQCCDEMVNKWERMVS-KEGQCELDVIPYLQTMTVDAISRTT 139
           IINPAFHLEKLK+MVPAF+QCC E+V  W+++VS K   CE+DV P L +MT D ISRT 
Sbjct: 154 IINPAFHLEKLKNMVPAFHQCCSEVVGAWDKLVSDKRSSCEVDVWPGLVSMTADMISRTA 213

Query: 140 FGSSYEKGKKIFELQKQLNFSVIKYLSMMTNPIWRFLPTKSKNKMKKMGKEIKSLILGII 199
           FGSSY++G++IFELQ+++   +I+ L     P + +LPTK   +MK   +EIK ++ GI+
Sbjct: 214 FGSSYKEGQRIFELQEEIKELLIQSLGKAFIPGYHYLPTKGNRRMKAADREIKVILRGIV 273

Query: 200 NERQKAMEAGEAAYSDLLGILMESNLNEIRQHGNNKNVGMSIEDVIEECKLFYLAGQETT 259
           N+R +A EAGEA   DLLGIL+ESNL      G  K  GMSIEDV+EECKLFYLAGQETT
Sbjct: 274 NKRLRAREAGEAPSEDLLGILLESNL------GQAKGNGMSIEDVMEECKLFYLAGQETT 333

Query: 260 ATLLIWTMILLSSYSEWQERARAEVREVFDNRKPDYNGLSRLKIVTMILNEVLRLYPPVS 319
           + LL+WTM++LS + +WQ RAR EV++VF +++P+  GL++LK++TMIL EVLRLYPPV+
Sbjct: 334 SVLLVWTMVMLSQHQDWQARAREEVKQVFGDKEPNTEGLNQLKVMTMILYEVLRLYPPVT 393

Query: 320 VLDRVVEKEMKLGNLSLPAGVILRLPIIFIHHDRELWGEDALEFNPQRFFDGVSKATKNQ 379
            L R + KEMKLG+++LPAGV + LPI+ +  D ELWG DA EF P+RF DG+SKA KNQ
Sbjct: 394 QLPRAIHKEMKLGDMTLPAGVHINLPIMLVQRDTELWGNDAAEFKPERFKDGLSKAAKNQ 453

Query: 380 TIFIPFGLGPRTCLGQNFAMIEAKMALSTILQQFSFQLSPSYTHAPFVTITTQPQHGAHI 439
             F  F  GPR C+GQNFA++EAKMA++ ILQ+FS +LSPSY HAP+  IT  PQ GAH+
Sbjct: 454 VSFFSFAWGPRICIGQNFALMEAKMAMALILQRFSLELSPSYVHAPYSVITLHPQFGAHL 512

Query: 440 ILHKL 444
           ILHKL
Sbjct: 514 ILHKL 512

[Close]

BLAST of MC11g_new0170 vs. ExPASy Swiss-Prot
Match: Q9LUC8 (Cytochrome P450 72A13 OS=Arabidopsis thaliana OX=3702 GN=CYP72A13 PE=2 SV=1)

HSP 1 Score: 503.4 bits (1295), Expect = 2.5e-141
Identity = 242/422 (57.35%), Postives = 318/422 (75.36%), Query Frame = 0


Query: 23  FTWNGPIPSVLTMEPEQVKTAFMQVDDFRKPKMDHLIKLIADGLVNHEGPKWEKHRKIIN 82
           FTW GPIP++  M+PEQ+K  F +V DF+K     L +LIA GLV+++G KW KHR+IIN
Sbjct: 97  FTWFGPIPTITIMDPEQIKEVFNKVYDFQKAHTFPLGRLIAAGLVSYDGDKWTKHRRIIN 156

Query: 83  PAFHLEKLKDMVPAFYQCCDEMVNKWERMVS-KEGQCELDVIPYLQTMTVDAISRTTFGS 142
           PAFHLEK+K+MVPAF+Q C E+V +W+++V+ K+  CE+D+ P+L +MT D ISRT FGS
Sbjct: 157 PAFHLEKIKNMVPAFHQSCSEIVGEWDKLVTDKQSSCEVDIWPWLVSMTADVISRTAFGS 216

Query: 143 SYEKGKKIFELQKQLNFSVIKYLSMMTNPIWRFLPTKSKNKMKKMGKEIKSLILGIINER 202
           SY++G++IFELQ +L   +I+       P +R+ PTK   +MK   +EIK ++ GI+N+R
Sbjct: 217 SYKEGQRIFELQAELAQLIIQAFRKAIIPGYRYFPTKGNRRMKAAAREIKFILRGIVNKR 276

Query: 203 QKAMEAGEAAYSDLLGILMESNLNEIRQHGNNKNVGMSIEDVIEECKLFYLAGQETTATL 262
            +A EAGEA   DLLGIL+ESNL      G  K  GMS E+++EECKLFY AGQETT  L
Sbjct: 277 LRAREAGEAPSDDLLGILLESNL------GQTKGNGMSTEELMEECKLFYFAGQETTTVL 336

Query: 263 LIWTMILLSSYSEWQERARAEVREVFDNRKPDYNGLSRLKIVTMILNEVLRLYPPVSVLD 322
           L+WTM+LLS + +WQ RAR EV++VF +++PD  GL++LK++TMIL EVLRLYPPV  L 
Sbjct: 337 LVWTMVLLSQHQDWQARAREEVKQVFGDKEPDAEGLNQLKVMTMILYEVLRLYPPVVQLT 396

Query: 323 RVVEKEMKLGNLSLPAGVILRLPIIFIHHDRELWGEDALEFNPQRFFDGVSKATKNQTIF 382
           R + KEM+LG+L+LP GV + LPI+ I  DRELWG DA EF P RF DG+SKATKNQ  F
Sbjct: 397 RAIHKEMQLGDLTLPGGVQISLPILLIQRDRELWGNDAGEFKPDRFKDGLSKATKNQVSF 456

Query: 383 IPFGLGPRTCLGQNFAMIEAKMALSTILQQFSFQLSPSYTHAPFVTITTQPQHGAHIILH 442
            PF  GPR C+GQNFA++EAKMA++ IL++FSF+LSPSY HAP+  +TT PQ GA +ILH
Sbjct: 457 FPFAWGPRICIGQNFALLEAKMAMTLILRKFSFELSPSYVHAPYTVLTTHPQFGAPLILH 512

Query: 443 KL 444
           KL
Sbjct: 517 KL 512

[Close]

BLAST of MC11g_new0170 vs. ExPASy Swiss-Prot
Match: Q9LUC5 (Cytochrome P450 72A15 OS=Arabidopsis thaliana OX=3702 GN=CYP72A15 PE=2 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 5.7e-141
Identity = 241/422 (57.11%), Postives = 320/422 (75.83%), Query Frame = 0


Query: 23  FTWNGPIPSVLTMEPEQVKTAFMQVDDFRKPKMDHLIKLIADGLVNHEGPKWEKHRKIIN 82
           FTW GPIP++  M+PEQ+K  F +V DF+KP    L  +IA GL N++G KW KHR+IIN
Sbjct: 97  FTWFGPIPTITIMDPEQIKEVFNKVYDFQKPHTFPLATIIAKGLANYDGDKWAKHRRIIN 156

Query: 83  PAFHLEKLKDMVPAFYQCCDEMVNKWERMVSKEG-QCELDVIPYLQTMTVDAISRTTFGS 142
           PAFH+EK+K+MVPAF+Q C E+V +W+++VS +G  CE+DV P L +MT D ISRT FGS
Sbjct: 157 PAFHIEKIKNMVPAFHQSCREVVGEWDQLVSDKGSSCEVDVWPGLVSMTADVISRTAFGS 216

Query: 143 SYEKGKKIFELQKQLNFSVIKYLSMMTNPIWRFLPTKSKNKMKKMGKEIKSLILGIINER 202
           SY++G++IFELQ +L   +I+       P + +LPTKS  +MK   +EI+ ++ GI+N+R
Sbjct: 217 SYKEGQRIFELQAELAQLIIQAFRKAFIPGYSYLPTKSNRRMKAAAREIQVILRGIVNKR 276

Query: 203 QKAMEAGEAAYSDLLGILMESNLNEIRQHGNNKNVGMSIEDVIEECKLFYLAGQETTATL 262
            +A EAGEA   DLLGIL+ESNL +    GN    GMS ED++EECKLFY AGQETT+ L
Sbjct: 277 LRAREAGEAPSDDLLGILLESNLRQ--TEGN----GMSTEDLMEECKLFYFAGQETTSVL 336

Query: 263 LIWTMILLSSYSEWQERARAEVREVFDNRKPDYNGLSRLKIVTMILNEVLRLYPPVSVLD 322
           L+WTM+LLS + +WQ RAR EV++VF +++PD  GL++LK++TMIL EVLRLYPPV+ L 
Sbjct: 337 LVWTMVLLSQHQDWQARAREEVKQVFGDKEPDAEGLNQLKVMTMILYEVLRLYPPVTQLT 396

Query: 323 RVVEKEMKLGNLSLPAGVILRLPIIFIHHDRELWGEDALEFNPQRFFDGVSKATKNQTIF 382
           R + KE+KLG+L+LP GV + LPI+ + HD ELWG DA EFNP RF DG+SKATK+Q  F
Sbjct: 397 RAIHKELKLGDLTLPGGVQISLPILLVQHDIELWGNDAAEFNPDRFKDGLSKATKSQVSF 456

Query: 383 IPFGLGPRTCLGQNFAMIEAKMALSTILQQFSFQLSPSYTHAPFVTITTQPQHGAHIILH 442
            PF  GPR C+GQNFA++EAKMA++ IL++FSF++SPSY HAP+  IT  PQ GA +I+H
Sbjct: 457 FPFAWGPRICIGQNFALLEAKMAMALILRRFSFEISPSYVHAPYTVITIHPQFGAQLIMH 512

Query: 443 KL 444
           KL
Sbjct: 517 KL 512

[Close]

BLAST of MC11g_new0170 vs. NCBI nr
Match: XP_022140103.1 (cytochrome P450 CYP72A219-like [Momordica charantia])

HSP 1 Score: 860 bits (2221), Expect = 5.33e-313
Identity = 425/425 (100.00%), Postives = 425/425 (100.00%), Query Frame = 0


Query: 20  KNSFTWNGPIPSVLTMEPEQVKTAFMQVDDFRKPKMDHLIKLIADGLVNHEGPKWEKHRK 79
           KNSFTWNGPIPSVLTMEPEQVKTAFMQVDDFRKPKMDHLIKLIADGLVNHEGPKWEKHRK
Sbjct: 96  KNSFTWNGPIPSVLTMEPEQVKTAFMQVDDFRKPKMDHLIKLIADGLVNHEGPKWEKHRK 155

Query: 80  IINPAFHLEKLKDMVPAFYQCCDEMVNKWERMVSKEGQCELDVIPYLQTMTVDAISRTTF 139
           IINPAFHLEKLKDMVPAFYQCCDEMVNKWERMVSKEGQCELDVIPYLQTMTVDAISRTTF
Sbjct: 156 IINPAFHLEKLKDMVPAFYQCCDEMVNKWERMVSKEGQCELDVIPYLQTMTVDAISRTTF 215

Query: 140 GSSYEKGKKIFELQKQLNFSVIKYLSMMTNPIWRFLPTKSKNKMKKMGKEIKSLILGIIN 199
           GSSYEKGKKIFELQKQLNFSVIKYLSMMTNPIWRFLPTKSKNKMKKMGKEIKSLILGIIN
Sbjct: 216 GSSYEKGKKIFELQKQLNFSVIKYLSMMTNPIWRFLPTKSKNKMKKMGKEIKSLILGIIN 275

Query: 200 ERQKAMEAGEAAYSDLLGILMESNLNEIRQHGNNKNVGMSIEDVIEECKLFYLAGQETTA 259
           ERQKAMEAGEAAYSDLLGILMESNLNEIRQHGNNKNVGMSIEDVIEECKLFYLAGQETTA
Sbjct: 276 ERQKAMEAGEAAYSDLLGILMESNLNEIRQHGNNKNVGMSIEDVIEECKLFYLAGQETTA 335

Query: 260 TLLIWTMILLSSYSEWQERARAEVREVFDNRKPDYNGLSRLKIVTMILNEVLRLYPPVSV 319
           TLLIWTMILLSSYSEWQERARAEVREVFDNRKPDYNGLSRLKIVTMILNEVLRLYPPVSV
Sbjct: 336 TLLIWTMILLSSYSEWQERARAEVREVFDNRKPDYNGLSRLKIVTMILNEVLRLYPPVSV 395

Query: 320 LDRVVEKEMKLGNLSLPAGVILRLPIIFIHHDRELWGEDALEFNPQRFFDGVSKATKNQT 379
           LDRVVEKEMKLGNLSLPAGVILRLPIIFIHHDRELWGEDALEFNPQRFFDGVSKATKNQT
Sbjct: 396 LDRVVEKEMKLGNLSLPAGVILRLPIIFIHHDRELWGEDALEFNPQRFFDGVSKATKNQT 455

Query: 380 IFIPFGLGPRTCLGQNFAMIEAKMALSTILQQFSFQLSPSYTHAPFVTITTQPQHGAHII 439
           IFIPFGLGPRTCLGQNFAMIEAKMALSTILQQFSFQLSPSYTHAPFVTITTQPQHGAHII
Sbjct: 456 IFIPFGLGPRTCLGQNFAMIEAKMALSTILQQFSFQLSPSYTHAPFVTITTQPQHGAHII 515

Query: 440 LHKLN 444
           LHKLN
Sbjct: 516 LHKLN 520

[Close]

BLAST of MC11g_new0170 vs. NCBI nr
Match: XP_022140083.1 (cytochrome P450 CYP72A219-like isoform X1 [Momordica charantia])

HSP 1 Score: 662 bits (1707), Expect = 8.91e-235
Identity = 329/426 (77.23%), Postives = 376/426 (88.26%), Query Frame = 0


Query: 20  KNSFTWNGPIPSVLTMEPEQVKTAFMQVDDFRKPKMDHLIKLIADGLVNHEGPKWEKHRK 79
           KNSF W+G IPSVL +EPEQVKTA +  DD RKPK+  L+KL+ADGLVNHEGPKW KHRK
Sbjct: 96  KNSFMWDGLIPSVLIVEPEQVKTALLS-DDIRKPKIKPLVKLLADGLVNHEGPKWVKHRK 155

Query: 80  IINPAFHLEKLKDMVPAFYQCCDEMVNKWERMVSKEGQCELDVIPYLQTMTVDAISRTTF 139
           IINPAFHLEKLKDMVPAF QCC++MVNKWE+MVSKEGQCEL+V+PYLQT+  D ISRT F
Sbjct: 156 IINPAFHLEKLKDMVPAFSQCCNKMVNKWEKMVSKEGQCELNVMPYLQTVAADMISRTAF 215

Query: 140 GSSYEKGKKIFELQKQLNFSVIKYLSMMTNPIWRFLPTKSKNKMKKMGKEIKSLILGIIN 199
           GSSYEKGK IFELQKQL+  V+K + M+TNPI RF PTKS +KM K+ KE+K LI  IIN
Sbjct: 216 GSSYEKGKMIFELQKQLSALVVKSM-MITNPILRFFPTKSNDKMVKINKEMKILISSIIN 275

Query: 200 ERQKAMEAGEAAYSDLLGILMESNLNEIRQ-HGNNKNVGMSIEDVIEECKLFYLAGQETT 259
           ER+K MEAGEAA +DLLGIL+ESNLNEIRQ HGNNK+ GMSIEDVIEECKLFYLAGQETT
Sbjct: 276 ERKKDMEAGEAACNDLLGILIESNLNEIRQKHGNNKDAGMSIEDVIEECKLFYLAGQETT 335

Query: 260 ATLLIWTMILLSSYSEWQERARAEVREVFDNRKPDYNGLSRLKIVTMILNEVLRLYPPVS 319
           ATLL+WTMILLSSYS+WQERARAEV +VF NR+PD++GLSRLKIVTMILNEVLRLYPPVS
Sbjct: 336 ATLLVWTMILLSSYSDWQERARAEVLQVFGNREPDFDGLSRLKIVTMILNEVLRLYPPVS 395

Query: 320 VLDRVVEKEMKLGNLSLPAGVILRLPIIFIHHDRELWGEDALEFNPQRFFDGVSKATKNQ 379
           +L+RVV+KE +LG ++LPAGV++RLPI+FI  D ELWGEDA EF P+RF +GVSKATKNQ
Sbjct: 396 MLERVVQKETRLGQITLPAGVMVRLPIVFIQQDPELWGEDAHEFKPERFSEGVSKATKNQ 455

Query: 380 TIFIPFGLGPRTCLGQNFAMIEAKMALSTILQQFSFQLSPSYTHAPFVTITTQPQHGAHI 439
           TIFIPFG GPR C+G NFAMIEAK  LS IL++FSFQLSPSY HAPF +++TQPQHGAHI
Sbjct: 456 TIFIPFGGGPRICIGLNFAMIEAKTTLSIILRRFSFQLSPSYIHAPFSSVSTQPQHGAHI 515

Query: 440 ILHKLN 444
           ILHKL+
Sbjct: 516 ILHKLD 519

[Close]

BLAST of MC11g_new0170 vs. NCBI nr
Match: XP_022156576.1 (cytochrome P450 CYP72A219-like [Momordica charantia])

HSP 1 Score: 651 bits (1679), Expect = 1.73e-230
Identity = 324/425 (76.24%), Postives = 364/425 (85.65%), Query Frame = 0


Query: 20  KNSFTWNGPIPSVLTMEPEQVKTAFMQVDDFRKPKMDHLIKLIADGLVNHEGPKWEKHRK 79
           KNSF W GPIP V  ME EQVKTA+M ++D +KP ++ L+KL+ADGLVNHEG KW KHRK
Sbjct: 96  KNSFMWIGPIPRVHIMEAEQVKTAYMLINDIQKPSINPLVKLLADGLVNHEGAKWVKHRK 155

Query: 80  IINPAFHLEKLKDMVPAFYQCCDEMVNKWERMVSKEGQCELDVIPYLQTMTVDAISRTTF 139
           IINPAFHLEKLKDMVPAF   C+EMV+KWERMVS EG CELDV+PYLQ+MT D ISRT F
Sbjct: 156 IINPAFHLEKLKDMVPAFSHSCNEMVDKWERMVSNEGLCELDVMPYLQSMTADIISRTAF 215

Query: 140 GSSYEKGKKIFELQKQLNFSVIKYLSMMTNPIWRFLPTKSKNKMKKMGKEIKSLILGIIN 199
           GSSYEKGK+IFELQK+L    IK    +  P  RFLPTKS  KMK+M KEIK LILGIIN
Sbjct: 216 GSSYEKGKRIFELQKELGVLAIKSTLRIYIPGSRFLPTKSNTKMKRMDKEIKGLILGIIN 275

Query: 200 ERQKAMEAGEAAYSDLLGILMESNLNEIRQHGNNKNVGMSIEDVIEECKLFYLAGQETTA 259
           ERQKAMEAGEA ++DLLGILMESNLNEIRQHGNNK+VGMSIEDVIEECKLFYLAGQETTA
Sbjct: 276 ERQKAMEAGEAIHNDLLGILMESNLNEIRQHGNNKDVGMSIEDVIEECKLFYLAGQETTA 335

Query: 260 TLLIWTMILLSSYSEWQERARAEVREVFDNRKPDYNGLSRLKIVTMILNEVLRLYPPVSV 319
           TLLIWTMILLSS+SEWQERARAEV +VF NR+PD++GLSRLK+VTMILNEVLRLYPPV +
Sbjct: 336 TLLIWTMILLSSHSEWQERARAEVLQVFGNREPDFDGLSRLKVVTMILNEVLRLYPPVGM 395

Query: 320 LDRVVEKEMKLGNLSLPAGVILRLPIIFIHHDRELWGEDALEFNPQRFFDGVSKATKN-Q 379
           + R+V+KE + G L+LPAGV+L LP I I HDRELWGEDA EF PQRF +GVSKATKN Q
Sbjct: 396 VSRLVQKETRFGQLTLPAGVMLGLPTILIQHDRELWGEDAQEFKPQRFSEGVSKATKNNQ 455

Query: 380 TIFIPFGLGPRTCLGQNFAMIEAKMALSTILQQFSFQLSPSYTHAPFVTITTQPQHGAHI 439
           T FIPFG GPR C+GQNFAMIEAK AL+ ILQ+FSF+LS SYTHAP  +IT QPQHGAH+
Sbjct: 456 TAFIPFGWGPRICIGQNFAMIEAKTALAIILQRFSFELSSSYTHAPTSSITIQPQHGAHV 515

Query: 440 ILHKL 443
           +LHKL
Sbjct: 516 VLHKL 520

[Close]

BLAST of MC11g_new0170 vs. NCBI nr
Match: KAA0064317.1 (cytochrome P450 CYP72A219-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 630 bits (1625), Expect = 1.47e-222
Identity = 308/426 (72.30%), Postives = 355/426 (83.33%), Query Frame = 0


Query: 20  KNSFTWNGPIPSVLTMEPEQVKTAFMQVDDFRKP--KMDHLIKLIADGLVNHEGPKWEKH 79
           KNSF W GPIP V  M+PEQ+KT F  ++D++KP   ++ L KL+ADGL+NHEG KW KH
Sbjct: 74  KNSFMWIGPIPRVHIMDPEQLKTVFSLINDYQKPTASLNPLAKLLADGLLNHEGHKWVKH 133

Query: 80  RKIINPAFHLEKLKDMVPAFYQCCDEMVNKWERMVSKEGQCELDVIPYLQTMTVDAISRT 139
           RKIINPAFHLEKLKDMVPAFY  CDEM++KWE MVS +G CELDV+PYLQ MT D ISRT
Sbjct: 134 RKIINPAFHLEKLKDMVPAFYHSCDEMISKWESMVSVQGSCELDVMPYLQNMTADVISRT 193

Query: 140 TFGSSYEKGKKIFELQKQLNFSVIKYLSMMTNPIWRFLPTKSKNKMKKMGKEIKSLILGI 199
            FGSSYEKGKKIF+LQ +L   VI+    +  P WRFLPTKS NKMK + KEI +LI+GI
Sbjct: 194 AFGSSYEKGKKIFKLQTELAEMVIRSTLGIYIPGWRFLPTKSNNKMKVISKEISTLIMGI 253

Query: 200 INERQKAMEAGEAAYSDLLGILMESNLNEIRQHGNNKNVGMSIEDVIEECKLFYLAGQET 259
           INER+ +M+AGEA  +DLL ILMESNLNEI+QHGNNK++GMSIEDVI+ECKLFY+AGQET
Sbjct: 254 INERETSMKAGEATQTDLLSILMESNLNEIKQHGNNKDIGMSIEDVIDECKLFYIAGQET 313

Query: 260 TATLLIWTMILLSSYSEWQERARAEVREVFDNRKPDYNGLSRLKIVTMILNEVLRLYPPV 319
           TATLL WTM+LLSSYSEWQERARAEV E+F N+KPDY+GLSRLK+VTMILNEVLRLYPPV
Sbjct: 314 TATLLTWTMVLLSSYSEWQERARAEVFEIFGNKKPDYDGLSRLKVVTMILNEVLRLYPPV 373

Query: 320 SVLDRVVEKEMKLGNLSLPAGVILRLPIIFIHHDRELWGEDALEFNPQRFFDGVSKATKN 379
           S+  R ++KE KLG L+LPAGV+L LP + I  D ELWGEDA EF P+RFF+GVSKATKN
Sbjct: 374 SMFGRFIKKETKLGKLTLPAGVMLGLPTVLIQCDPELWGEDAHEFKPERFFEGVSKATKN 433

Query: 380 QTIFIPFGLGPRTCLGQNFAMIEAKMALSTILQQFSFQLSPSYTHAPFVTITTQPQHGAH 439
              F+PFG GPR C+G NFAMIEAKM LS ILQ+FS +LS SYTHAP V ITTQPQHGAH
Sbjct: 434 SGAFVPFGWGPRICIGLNFAMIEAKMTLSMILQRFSLELSSSYTHAPIVAITTQPQHGAH 493

Query: 440 IILHKL 443
           IILHKL
Sbjct: 494 IILHKL 499

[Close]

BLAST of MC11g_new0170 vs. NCBI nr
Match: XP_023536197.1 (cytochrome P450 CYP72A219-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 630 bits (1626), Expect = 1.71e-222
Identity = 309/424 (72.88%), Postives = 353/424 (83.25%), Query Frame = 0


Query: 20  KNSFTWNGPIPSVLTMEPEQVKTAFMQVDDFRKPKMDHLIKLIADGLVNHEGPKWEKHRK 79
           KNSF W GPIP V  M+PEQ+KT F   +D +KP M+ L+KL A GLVNHEG KW KHRK
Sbjct: 94  KNSFMWLGPIPRVNIMDPEQLKTVFALFNDIQKPSMNPLVKLFAGGLVNHEGAKWAKHRK 153

Query: 80  IINPAFHLEKLKDMVPAFYQCCDEMVNKWERMVSKEGQCELDVIPYLQTMTVDAISRTTF 139
           IINPAFHLEKLK MVPAFY  C +MV+KWE MVS+EG C+LDV+PYLQ M  D ISRT F
Sbjct: 154 IINPAFHLEKLKGMVPAFYHSCYDMVSKWESMVSEEGSCDLDVMPYLQQMAADVISRTAF 213

Query: 140 GSSYEKGKKIFELQKQLNFSVIKYLSMMTNPIWRFLPTKSKNKMKKMGKEIKSLILGIIN 199
           GSSYEKGKKIF+LQ +L   VI+ +  +  P WRFLPTKS  KMK++ KEI SLILGIIN
Sbjct: 214 GSSYEKGKKIFKLQTELADLVIQTIMRIYIPGWRFLPTKSNKKMKEISKEISSLILGIIN 273

Query: 200 ERQKAMEAGEAAYSDLLGILMESNLNEIRQHGNNKNVGMSIEDVIEECKLFYLAGQETTA 259
           ER+K+M+AGEA  SDLLGILMESNLNEI QHGNNK++GMSIEDVI+ECK+FY+AGQETTA
Sbjct: 274 EREKSMKAGEAMNSDLLGILMESNLNEIGQHGNNKDIGMSIEDVIDECKIFYIAGQETTA 333

Query: 260 TLLIWTMILLSSYSEWQERARAEVREVFDNRKPDYNGLSRLKIVTMILNEVLRLYPPVSV 319
           TLLIWTMILLSSYSEWQ+RARAEV E+F N+ PDY+GL+RLK+VTMI NEVLRLY PV +
Sbjct: 334 TLLIWTMILLSSYSEWQDRARAEVFEIFGNKNPDYDGLNRLKVVTMIFNEVLRLYTPVGM 393

Query: 320 LDRVVEKEMKLGNLSLPAGVILRLPIIFIHHDRELWGEDALEFNPQRFFDGVSKATKNQT 379
           L R V+KE KLGNL++PAGV L LP++ IH D ELWGEDA EF P+RF +GVSKATKN  
Sbjct: 394 LGRHVKKETKLGNLTVPAGVFLGLPVLLIHRDPELWGEDADEFKPERFSEGVSKATKNPA 453

Query: 380 IFIPFGLGPRTCLGQNFAMIEAKMALSTILQQFSFQLSPSYTHAPFVTITTQPQHGAHII 439
            FIPFG GPR C+G NFAMIEAKMALS ILQ+FSF+LSPSYTHAP   ITTQPQHGAHI+
Sbjct: 454 AFIPFGWGPRICIGLNFAMIEAKMALSIILQRFSFELSPSYTHAPTAAITTQPQHGAHIM 513

Query: 440 LHKL 443
           LHKL
Sbjct: 514 LHKL 517

[Close]

BLAST of MC11g_new0170 vs. ExPASy TrEMBL
Match: A0A6J1CH88 (cytochrome P450 CYP72A219-like OS=Momordica charantia OX=3673 GN=LOC111010835 PE=3 SV=1)

HSP 1 Score: 860 bits (2221), Expect = 2.58e-313
Identity = 425/425 (100.00%), Postives = 425/425 (100.00%), Query Frame = 0


Query: 20  KNSFTWNGPIPSVLTMEPEQVKTAFMQVDDFRKPKMDHLIKLIADGLVNHEGPKWEKHRK 79
           KNSFTWNGPIPSVLTMEPEQVKTAFMQVDDFRKPKMDHLIKLIADGLVNHEGPKWEKHRK
Sbjct: 96  KNSFTWNGPIPSVLTMEPEQVKTAFMQVDDFRKPKMDHLIKLIADGLVNHEGPKWEKHRK 155

Query: 80  IINPAFHLEKLKDMVPAFYQCCDEMVNKWERMVSKEGQCELDVIPYLQTMTVDAISRTTF 139
           IINPAFHLEKLKDMVPAFYQCCDEMVNKWERMVSKEGQCELDVIPYLQTMTVDAISRTTF
Sbjct: 156 IINPAFHLEKLKDMVPAFYQCCDEMVNKWERMVSKEGQCELDVIPYLQTMTVDAISRTTF 215

Query: 140 GSSYEKGKKIFELQKQLNFSVIKYLSMMTNPIWRFLPTKSKNKMKKMGKEIKSLILGIIN 199
           GSSYEKGKKIFELQKQLNFSVIKYLSMMTNPIWRFLPTKSKNKMKKMGKEIKSLILGIIN
Sbjct: 216 GSSYEKGKKIFELQKQLNFSVIKYLSMMTNPIWRFLPTKSKNKMKKMGKEIKSLILGIIN 275

Query: 200 ERQKAMEAGEAAYSDLLGILMESNLNEIRQHGNNKNVGMSIEDVIEECKLFYLAGQETTA 259
           ERQKAMEAGEAAYSDLLGILMESNLNEIRQHGNNKNVGMSIEDVIEECKLFYLAGQETTA
Sbjct: 276 ERQKAMEAGEAAYSDLLGILMESNLNEIRQHGNNKNVGMSIEDVIEECKLFYLAGQETTA 335

Query: 260 TLLIWTMILLSSYSEWQERARAEVREVFDNRKPDYNGLSRLKIVTMILNEVLRLYPPVSV 319
           TLLIWTMILLSSYSEWQERARAEVREVFDNRKPDYNGLSRLKIVTMILNEVLRLYPPVSV
Sbjct: 336 TLLIWTMILLSSYSEWQERARAEVREVFDNRKPDYNGLSRLKIVTMILNEVLRLYPPVSV 395

Query: 320 LDRVVEKEMKLGNLSLPAGVILRLPIIFIHHDRELWGEDALEFNPQRFFDGVSKATKNQT 379
           LDRVVEKEMKLGNLSLPAGVILRLPIIFIHHDRELWGEDALEFNPQRFFDGVSKATKNQT
Sbjct: 396 LDRVVEKEMKLGNLSLPAGVILRLPIIFIHHDRELWGEDALEFNPQRFFDGVSKATKNQT 455

Query: 380 IFIPFGLGPRTCLGQNFAMIEAKMALSTILQQFSFQLSPSYTHAPFVTITTQPQHGAHII 439
           IFIPFGLGPRTCLGQNFAMIEAKMALSTILQQFSFQLSPSYTHAPFVTITTQPQHGAHII
Sbjct: 456 IFIPFGLGPRTCLGQNFAMIEAKMALSTILQQFSFQLSPSYTHAPFVTITTQPQHGAHII 515

Query: 440 LHKLN 444
           LHKLN
Sbjct: 516 LHKLN 520

[Close]

BLAST of MC11g_new0170 vs. ExPASy TrEMBL
Match: A0A6J1CH71 (cytochrome P450 CYP72A219-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010820 PE=3 SV=1)

HSP 1 Score: 662 bits (1707), Expect = 4.31e-235
Identity = 329/426 (77.23%), Postives = 376/426 (88.26%), Query Frame = 0


Query: 20  KNSFTWNGPIPSVLTMEPEQVKTAFMQVDDFRKPKMDHLIKLIADGLVNHEGPKWEKHRK 79
           KNSF W+G IPSVL +EPEQVKTA +  DD RKPK+  L+KL+ADGLVNHEGPKW KHRK
Sbjct: 96  KNSFMWDGLIPSVLIVEPEQVKTALLS-DDIRKPKIKPLVKLLADGLVNHEGPKWVKHRK 155

Query: 80  IINPAFHLEKLKDMVPAFYQCCDEMVNKWERMVSKEGQCELDVIPYLQTMTVDAISRTTF 139
           IINPAFHLEKLKDMVPAF QCC++MVNKWE+MVSKEGQCEL+V+PYLQT+  D ISRT F
Sbjct: 156 IINPAFHLEKLKDMVPAFSQCCNKMVNKWEKMVSKEGQCELNVMPYLQTVAADMISRTAF 215

Query: 140 GSSYEKGKKIFELQKQLNFSVIKYLSMMTNPIWRFLPTKSKNKMKKMGKEIKSLILGIIN 199
           GSSYEKGK IFELQKQL+  V+K + M+TNPI RF PTKS +KM K+ KE+K LI  IIN
Sbjct: 216 GSSYEKGKMIFELQKQLSALVVKSM-MITNPILRFFPTKSNDKMVKINKEMKILISSIIN 275

Query: 200 ERQKAMEAGEAAYSDLLGILMESNLNEIRQ-HGNNKNVGMSIEDVIEECKLFYLAGQETT 259
           ER+K MEAGEAA +DLLGIL+ESNLNEIRQ HGNNK+ GMSIEDVIEECKLFYLAGQETT
Sbjct: 276 ERKKDMEAGEAACNDLLGILIESNLNEIRQKHGNNKDAGMSIEDVIEECKLFYLAGQETT 335

Query: 260 ATLLIWTMILLSSYSEWQERARAEVREVFDNRKPDYNGLSRLKIVTMILNEVLRLYPPVS 319
           ATLL+WTMILLSSYS+WQERARAEV +VF NR+PD++GLSRLKIVTMILNEVLRLYPPVS
Sbjct: 336 ATLLVWTMILLSSYSDWQERARAEVLQVFGNREPDFDGLSRLKIVTMILNEVLRLYPPVS 395

Query: 320 VLDRVVEKEMKLGNLSLPAGVILRLPIIFIHHDRELWGEDALEFNPQRFFDGVSKATKNQ 379
           +L+RVV+KE +LG ++LPAGV++RLPI+FI  D ELWGEDA EF P+RF +GVSKATKNQ
Sbjct: 396 MLERVVQKETRLGQITLPAGVMVRLPIVFIQQDPELWGEDAHEFKPERFSEGVSKATKNQ 455

Query: 380 TIFIPFGLGPRTCLGQNFAMIEAKMALSTILQQFSFQLSPSYTHAPFVTITTQPQHGAHI 439
           TIFIPFG GPR C+G NFAMIEAK  LS IL++FSFQLSPSY HAPF +++TQPQHGAHI
Sbjct: 456 TIFIPFGGGPRICIGLNFAMIEAKTTLSIILRRFSFQLSPSYIHAPFSSVSTQPQHGAHI 515

Query: 440 ILHKLN 444
           ILHKL+
Sbjct: 516 ILHKLD 519

[Close]

BLAST of MC11g_new0170 vs. ExPASy TrEMBL
Match: A0A6J1DVD0 (cytochrome P450 CYP72A219-like OS=Momordica charantia OX=3673 GN=LOC111023453 PE=3 SV=1)

HSP 1 Score: 651 bits (1679), Expect = 8.38e-231
Identity = 324/425 (76.24%), Postives = 364/425 (85.65%), Query Frame = 0


Query: 20  KNSFTWNGPIPSVLTMEPEQVKTAFMQVDDFRKPKMDHLIKLIADGLVNHEGPKWEKHRK 79
           KNSF W GPIP V  ME EQVKTA+M ++D +KP ++ L+KL+ADGLVNHEG KW KHRK
Sbjct: 96  KNSFMWIGPIPRVHIMEAEQVKTAYMLINDIQKPSINPLVKLLADGLVNHEGAKWVKHRK 155

Query: 80  IINPAFHLEKLKDMVPAFYQCCDEMVNKWERMVSKEGQCELDVIPYLQTMTVDAISRTTF 139
           IINPAFHLEKLKDMVPAF   C+EMV+KWERMVS EG CELDV+PYLQ+MT D ISRT F
Sbjct: 156 IINPAFHLEKLKDMVPAFSHSCNEMVDKWERMVSNEGLCELDVMPYLQSMTADIISRTAF 215

Query: 140 GSSYEKGKKIFELQKQLNFSVIKYLSMMTNPIWRFLPTKSKNKMKKMGKEIKSLILGIIN 199
           GSSYEKGK+IFELQK+L    IK    +  P  RFLPTKS  KMK+M KEIK LILGIIN
Sbjct: 216 GSSYEKGKRIFELQKELGVLAIKSTLRIYIPGSRFLPTKSNTKMKRMDKEIKGLILGIIN 275

Query: 200 ERQKAMEAGEAAYSDLLGILMESNLNEIRQHGNNKNVGMSIEDVIEECKLFYLAGQETTA 259
           ERQKAMEAGEA ++DLLGILMESNLNEIRQHGNNK+VGMSIEDVIEECKLFYLAGQETTA
Sbjct: 276 ERQKAMEAGEAIHNDLLGILMESNLNEIRQHGNNKDVGMSIEDVIEECKLFYLAGQETTA 335

Query: 260 TLLIWTMILLSSYSEWQERARAEVREVFDNRKPDYNGLSRLKIVTMILNEVLRLYPPVSV 319
           TLLIWTMILLSS+SEWQERARAEV +VF NR+PD++GLSRLK+VTMILNEVLRLYPPV +
Sbjct: 336 TLLIWTMILLSSHSEWQERARAEVLQVFGNREPDFDGLSRLKVVTMILNEVLRLYPPVGM 395

Query: 320 LDRVVEKEMKLGNLSLPAGVILRLPIIFIHHDRELWGEDALEFNPQRFFDGVSKATKN-Q 379
           + R+V+KE + G L+LPAGV+L LP I I HDRELWGEDA EF PQRF +GVSKATKN Q
Sbjct: 396 VSRLVQKETRFGQLTLPAGVMLGLPTILIQHDRELWGEDAQEFKPQRFSEGVSKATKNNQ 455

Query: 380 TIFIPFGLGPRTCLGQNFAMIEAKMALSTILQQFSFQLSPSYTHAPFVTITTQPQHGAHI 439
           T FIPFG GPR C+GQNFAMIEAK AL+ ILQ+FSF+LS SYTHAP  +IT QPQHGAH+
Sbjct: 456 TAFIPFGWGPRICIGQNFAMIEAKTALAIILQRFSFELSSSYTHAPTSSITIQPQHGAHV 515

Query: 440 ILHKL 443
           +LHKL
Sbjct: 516 VLHKL 520

[Close]

BLAST of MC11g_new0170 vs. ExPASy TrEMBL
Match: A0A5A7VBB4 (Cytochrome P450 CYP72A219-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G00160 PE=3 SV=1)

HSP 1 Score: 630 bits (1625), Expect = 7.11e-223
Identity = 308/426 (72.30%), Postives = 355/426 (83.33%), Query Frame = 0


Query: 20  KNSFTWNGPIPSVLTMEPEQVKTAFMQVDDFRKP--KMDHLIKLIADGLVNHEGPKWEKH 79
           KNSF W GPIP V  M+PEQ+KT F  ++D++KP   ++ L KL+ADGL+NHEG KW KH
Sbjct: 74  KNSFMWIGPIPRVHIMDPEQLKTVFSLINDYQKPTASLNPLAKLLADGLLNHEGHKWVKH 133

Query: 80  RKIINPAFHLEKLKDMVPAFYQCCDEMVNKWERMVSKEGQCELDVIPYLQTMTVDAISRT 139
           RKIINPAFHLEKLKDMVPAFY  CDEM++KWE MVS +G CELDV+PYLQ MT D ISRT
Sbjct: 134 RKIINPAFHLEKLKDMVPAFYHSCDEMISKWESMVSVQGSCELDVMPYLQNMTADVISRT 193

Query: 140 TFGSSYEKGKKIFELQKQLNFSVIKYLSMMTNPIWRFLPTKSKNKMKKMGKEIKSLILGI 199
            FGSSYEKGKKIF+LQ +L   VI+    +  P WRFLPTKS NKMK + KEI +LI+GI
Sbjct: 194 AFGSSYEKGKKIFKLQTELAEMVIRSTLGIYIPGWRFLPTKSNNKMKVISKEISTLIMGI 253

Query: 200 INERQKAMEAGEAAYSDLLGILMESNLNEIRQHGNNKNVGMSIEDVIEECKLFYLAGQET 259
           INER+ +M+AGEA  +DLL ILMESNLNEI+QHGNNK++GMSIEDVI+ECKLFY+AGQET
Sbjct: 254 INERETSMKAGEATQTDLLSILMESNLNEIKQHGNNKDIGMSIEDVIDECKLFYIAGQET 313

Query: 260 TATLLIWTMILLSSYSEWQERARAEVREVFDNRKPDYNGLSRLKIVTMILNEVLRLYPPV 319
           TATLL WTM+LLSSYSEWQERARAEV E+F N+KPDY+GLSRLK+VTMILNEVLRLYPPV
Sbjct: 314 TATLLTWTMVLLSSYSEWQERARAEVFEIFGNKKPDYDGLSRLKVVTMILNEVLRLYPPV 373

Query: 320 SVLDRVVEKEMKLGNLSLPAGVILRLPIIFIHHDRELWGEDALEFNPQRFFDGVSKATKN 379
           S+  R ++KE KLG L+LPAGV+L LP + I  D ELWGEDA EF P+RFF+GVSKATKN
Sbjct: 374 SMFGRFIKKETKLGKLTLPAGVMLGLPTVLIQCDPELWGEDAHEFKPERFFEGVSKATKN 433

Query: 380 QTIFIPFGLGPRTCLGQNFAMIEAKMALSTILQQFSFQLSPSYTHAPFVTITTQPQHGAH 439
              F+PFG GPR C+G NFAMIEAKM LS ILQ+FS +LS SYTHAP V ITTQPQHGAH
Sbjct: 434 SGAFVPFGWGPRICIGLNFAMIEAKMTLSMILQRFSLELSSSYTHAPIVAITTQPQHGAH 493

Query: 440 IILHKL 443
           IILHKL
Sbjct: 494 IILHKL 499

[Close]

BLAST of MC11g_new0170 vs. ExPASy TrEMBL
Match: A0A1S3BT76 (cytochrome P450 CYP72A219-like OS=Cucumis melo OX=3656 GN=LOC103493471 PE=3 SV=1)

HSP 1 Score: 630 bits (1625), Expect = 1.80e-222
Identity = 308/426 (72.30%), Postives = 355/426 (83.33%), Query Frame = 0


Query: 20  KNSFTWNGPIPSVLTMEPEQVKTAFMQVDDFRKP--KMDHLIKLIADGLVNHEGPKWEKH 79
           KNSF W GPIP V  M+PEQ+KT F  ++D++KP   ++ L KL+ADGL+NHEG KW KH
Sbjct: 100 KNSFMWIGPIPRVHIMDPEQLKTVFSLINDYQKPTASLNPLAKLLADGLLNHEGHKWVKH 159

Query: 80  RKIINPAFHLEKLKDMVPAFYQCCDEMVNKWERMVSKEGQCELDVIPYLQTMTVDAISRT 139
           RKIINPAFHLEKLKDMVPAFY  CDEM++KWE MVS +G CELDV+PYLQ MT D ISRT
Sbjct: 160 RKIINPAFHLEKLKDMVPAFYHSCDEMISKWESMVSVQGSCELDVMPYLQNMTADVISRT 219

Query: 140 TFGSSYEKGKKIFELQKQLNFSVIKYLSMMTNPIWRFLPTKSKNKMKKMGKEIKSLILGI 199
            FGSSYEKGKKIF+LQ +L   VI+    +  P WRFLPTKS NKMK + KEI +LI+GI
Sbjct: 220 AFGSSYEKGKKIFKLQTELAEMVIRSTLGIYIPGWRFLPTKSNNKMKVISKEISTLIMGI 279

Query: 200 INERQKAMEAGEAAYSDLLGILMESNLNEIRQHGNNKNVGMSIEDVIEECKLFYLAGQET 259
           INER+ +M+AGEA  +DLL ILMESNLNEI+QHGNNK++GMSIEDVI+ECKLFY+AGQET
Sbjct: 280 INERETSMKAGEATQTDLLSILMESNLNEIKQHGNNKDIGMSIEDVIDECKLFYIAGQET 339

Query: 260 TATLLIWTMILLSSYSEWQERARAEVREVFDNRKPDYNGLSRLKIVTMILNEVLRLYPPV 319
           TATLL WTM+LLSSYSEWQERARAEV E+F N+KPDY+GLSRLK+VTMILNEVLRLYPPV
Sbjct: 340 TATLLTWTMVLLSSYSEWQERARAEVFEIFGNKKPDYDGLSRLKVVTMILNEVLRLYPPV 399

Query: 320 SVLDRVVEKEMKLGNLSLPAGVILRLPIIFIHHDRELWGEDALEFNPQRFFDGVSKATKN 379
           S+  R ++KE KLG L+LPAGV+L LP + I  D ELWGEDA EF P+RFF+GVSKATKN
Sbjct: 400 SMFGRFIKKETKLGKLTLPAGVMLGLPTVLIQCDPELWGEDAHEFKPERFFEGVSKATKN 459

Query: 380 QTIFIPFGLGPRTCLGQNFAMIEAKMALSTILQQFSFQLSPSYTHAPFVTITTQPQHGAH 439
              F+PFG GPR C+G NFAMIEAKM LS ILQ+FS +LS SYTHAP V ITTQPQHGAH
Sbjct: 460 SGAFVPFGWGPRICIGLNFAMIEAKMTLSMILQRFSLELSSSYTHAPIVAITTQPQHGAH 519

Query: 440 IILHKL 443
           IILHKL
Sbjct: 520 IILHKL 525

[Close]

BLAST of MC11g_new0170 vs. TAIR 10
Match: AT3G14660.1 (cytochrome P450, family 72, subfamily A, polypeptide 13 )

HSP 1 Score: 503.4 bits (1295), Expect = 1.8e-142
Identity = 242/422 (57.35%), Postives = 318/422 (75.36%), Query Frame = 0


Query: 23  FTWNGPIPSVLTMEPEQVKTAFMQVDDFRKPKMDHLIKLIADGLVNHEGPKWEKHRKIIN 82
           FTW GPIP++  M+PEQ+K  F +V DF+K     L +LIA GLV+++G KW KHR+IIN
Sbjct: 97  FTWFGPIPTITIMDPEQIKEVFNKVYDFQKAHTFPLGRLIAAGLVSYDGDKWTKHRRIIN 156

Query: 83  PAFHLEKLKDMVPAFYQCCDEMVNKWERMVS-KEGQCELDVIPYLQTMTVDAISRTTFGS 142
           PAFHLEK+K+MVPAF+Q C E+V +W+++V+ K+  CE+D+ P+L +MT D ISRT FGS
Sbjct: 157 PAFHLEKIKNMVPAFHQSCSEIVGEWDKLVTDKQSSCEVDIWPWLVSMTADVISRTAFGS 216

Query: 143 SYEKGKKIFELQKQLNFSVIKYLSMMTNPIWRFLPTKSKNKMKKMGKEIKSLILGIINER 202
           SY++G++IFELQ +L   +I+       P +R+ PTK   +MK   +EIK ++ GI+N+R
Sbjct: 217 SYKEGQRIFELQAELAQLIIQAFRKAIIPGYRYFPTKGNRRMKAAAREIKFILRGIVNKR 276

Query: 203 QKAMEAGEAAYSDLLGILMESNLNEIRQHGNNKNVGMSIEDVIEECKLFYLAGQETTATL 262
            +A EAGEA   DLLGIL+ESNL      G  K  GMS E+++EECKLFY AGQETT  L
Sbjct: 277 LRAREAGEAPSDDLLGILLESNL------GQTKGNGMSTEELMEECKLFYFAGQETTTVL 336

Query: 263 LIWTMILLSSYSEWQERARAEVREVFDNRKPDYNGLSRLKIVTMILNEVLRLYPPVSVLD 322
           L+WTM+LLS + +WQ RAR EV++VF +++PD  GL++LK++TMIL EVLRLYPPV  L 
Sbjct: 337 LVWTMVLLSQHQDWQARAREEVKQVFGDKEPDAEGLNQLKVMTMILYEVLRLYPPVVQLT 396

Query: 323 RVVEKEMKLGNLSLPAGVILRLPIIFIHHDRELWGEDALEFNPQRFFDGVSKATKNQTIF 382
           R + KEM+LG+L+LP GV + LPI+ I  DRELWG DA EF P RF DG+SKATKNQ  F
Sbjct: 397 RAIHKEMQLGDLTLPGGVQISLPILLIQRDRELWGNDAGEFKPDRFKDGLSKATKNQVSF 456

Query: 383 IPFGLGPRTCLGQNFAMIEAKMALSTILQQFSFQLSPSYTHAPFVTITTQPQHGAHIILH 442
            PF  GPR C+GQNFA++EAKMA++ IL++FSF+LSPSY HAP+  +TT PQ GA +ILH
Sbjct: 457 FPFAWGPRICIGQNFALLEAKMAMTLILRKFSFELSPSYVHAPYTVLTTHPQFGAPLILH 512

Query: 443 KL 444
           KL
Sbjct: 517 KL 512

[Close]

BLAST of MC11g_new0170 vs. TAIR 10
Match: AT3G14690.1 (cytochrome P450, family 72, subfamily A, polypeptide 15 )

HSP 1 Score: 502.3 bits (1292), Expect = 4.0e-142
Identity = 241/422 (57.11%), Postives = 320/422 (75.83%), Query Frame = 0


Query: 23  FTWNGPIPSVLTMEPEQVKTAFMQVDDFRKPKMDHLIKLIADGLVNHEGPKWEKHRKIIN 82
           FTW GPIP++  M+PEQ+K  F +V DF+KP    L  +IA GL N++G KW KHR+IIN
Sbjct: 97  FTWFGPIPTITIMDPEQIKEVFNKVYDFQKPHTFPLATIIAKGLANYDGDKWAKHRRIIN 156

Query: 83  PAFHLEKLKDMVPAFYQCCDEMVNKWERMVSKEG-QCELDVIPYLQTMTVDAISRTTFGS 142
           PAFH+EK+K+MVPAF+Q C E+V +W+++VS +G  CE+DV P L +MT D ISRT FGS
Sbjct: 157 PAFHIEKIKNMVPAFHQSCREVVGEWDQLVSDKGSSCEVDVWPGLVSMTADVISRTAFGS 216

Query: 143 SYEKGKKIFELQKQLNFSVIKYLSMMTNPIWRFLPTKSKNKMKKMGKEIKSLILGIINER 202
           SY++G++IFELQ +L   +I+       P + +LPTKS  +MK   +EI+ ++ GI+N+R
Sbjct: 217 SYKEGQRIFELQAELAQLIIQAFRKAFIPGYSYLPTKSNRRMKAAAREIQVILRGIVNKR 276

Query: 203 QKAMEAGEAAYSDLLGILMESNLNEIRQHGNNKNVGMSIEDVIEECKLFYLAGQETTATL 262
            +A EAGEA   DLLGIL+ESNL +    GN    GMS ED++EECKLFY AGQETT+ L
Sbjct: 277 LRAREAGEAPSDDLLGILLESNLRQ--TEGN----GMSTEDLMEECKLFYFAGQETTSVL 336

Query: 263 LIWTMILLSSYSEWQERARAEVREVFDNRKPDYNGLSRLKIVTMILNEVLRLYPPVSVLD 322
           L+WTM+LLS + +WQ RAR EV++VF +++PD  GL++LK++TMIL EVLRLYPPV+ L 
Sbjct: 337 LVWTMVLLSQHQDWQARAREEVKQVFGDKEPDAEGLNQLKVMTMILYEVLRLYPPVTQLT 396

Query: 323 RVVEKEMKLGNLSLPAGVILRLPIIFIHHDRELWGEDALEFNPQRFFDGVSKATKNQTIF 382
           R + KE+KLG+L+LP GV + LPI+ + HD ELWG DA EFNP RF DG+SKATK+Q  F
Sbjct: 397 RAIHKELKLGDLTLPGGVQISLPILLVQHDIELWGNDAAEFNPDRFKDGLSKATKSQVSF 456

Query: 383 IPFGLGPRTCLGQNFAMIEAKMALSTILQQFSFQLSPSYTHAPFVTITTQPQHGAHIILH 442
            PF  GPR C+GQNFA++EAKMA++ IL++FSF++SPSY HAP+  IT  PQ GA +I+H
Sbjct: 457 FPFAWGPRICIGQNFALLEAKMAMALILRRFSFEISPSYVHAPYTVITIHPQFGAQLIMH 512

Query: 443 KL 444
           KL
Sbjct: 517 KL 512

[Close]

BLAST of MC11g_new0170 vs. TAIR 10
Match: AT3G14680.1 (cytochrome P450, family 72, subfamily A, polypeptide 14 )

HSP 1 Score: 496.5 bits (1277), Expect = 2.2e-140
Identity = 237/425 (55.76%), Postives = 316/425 (74.35%), Query Frame = 0


Query: 20  KNSFTWNGPIPSVLTMEPEQVKTAFMQVDDFRKPKMDHLIKLIADGLVNHEGPKWEKHRK 79
           + + TW GPIP++  M+PEQ+K  F +V DF+K     L K++  GLV+++G KW +HR+
Sbjct: 94  RTNLTWFGPIPTITIMDPEQIKEVFNKVYDFQKAHTFPLSKILGTGLVSYDGDKWAQHRR 153

Query: 80  IINPAFHLEKLKDMVPAFYQCCDEMVNKWERMVSKEG-QCELDVIPYLQTMTVDAISRTT 139
           IINPAFHLEK+K+MV  F++ C E+V +W+++VS +G  CE+DV P L +MT D ISRT 
Sbjct: 154 IINPAFHLEKIKNMVHVFHESCSELVGEWDKLVSDKGSSCEVDVWPGLTSMTADVISRTA 213

Query: 140 FGSSYEKGKKIFELQKQLNFSVIKYLSMMTNPIWRFLPTKSKNKMKKMGKEIKSLILGII 199
           FGSSY +G +IFELQ +L   V++       P + +LPTK   +MK   +EI+ ++ GII
Sbjct: 214 FGSSYREGHRIFELQAELAQLVMQAFQKFFIPGYIYLPTKGNRRMKTAAREIQDILRGII 273

Query: 200 NERQKAMEAGEAAYSDLLGILMESNLNEIRQHGNNKNVGMSIEDVIEECKLFYLAGQETT 259
           N+R++A E+GEA   DLLGIL+ESNL      G  +  GMS ED++EECKLFYLAGQETT
Sbjct: 274 NKRERARESGEAPSEDLLGILLESNL------GQTEGNGMSTEDMMEECKLFYLAGQETT 333

Query: 260 ATLLIWTMILLSSYSEWQERARAEVREVFDNRKPDYNGLSRLKIVTMILNEVLRLYPPVS 319
           + LL+WTM+LLS + +WQ RAR EV++VF +++PD  GL++LK++TMIL EVLRLYPPV 
Sbjct: 334 SVLLVWTMVLLSQHQDWQARAREEVKQVFGDKQPDTEGLNQLKVMTMILYEVLRLYPPVV 393

Query: 320 VLDRVVEKEMKLGNLSLPAGVILRLPIIFIHHDRELWGEDALEFNPQRFFDGVSKATKNQ 379
            L R + KEMKLG+L+LP GV + LP++ +H D ELWG DA EF P+RF DG+SKATKNQ
Sbjct: 394 QLTRAIHKEMKLGDLTLPGGVQISLPVLLVHRDTELWGNDAGEFKPERFKDGLSKATKNQ 453

Query: 380 TIFIPFGLGPRTCLGQNFAMIEAKMALSTILQQFSFQLSPSYTHAPFVTITTQPQHGAHI 439
             F PF  GPR C+GQNF ++EAKMA+S ILQ+FSF+LSPSY HAP+  IT  PQ GAH+
Sbjct: 454 VSFFPFAWGPRICIGQNFTLLEAKMAMSLILQRFSFELSPSYVHAPYTIITLYPQFGAHL 512

Query: 440 ILHKL 444
           +LHKL
Sbjct: 514 MLHKL 512

[Close]

BLAST of MC11g_new0170 vs. TAIR 10
Match: AT3G14610.1 (cytochrome P450, family 72, subfamily A, polypeptide 7 )

HSP 1 Score: 495.0 bits (1273), Expect = 6.4e-140
Identity = 241/426 (56.57%), Postives = 313/426 (73.47%), Query Frame = 0


Query: 20  KNSFTWNGPIPSVLTMEPEQVKTAFMQVDDFRKPKMDHLIKLIADGLVNHEGPKWEKHRK 79
           K  F W GP+P+++   PEQ+K  F +V+DF K     LI+L+A GL +++G KW  HR+
Sbjct: 93  KTFFIWIGPLPTIVITNPEQIKEVFNKVNDFEKASTFPLIRLLAGGLASYKGDKWASHRR 152

Query: 80  IINPAFHLEKLKDMVPAFYQCCDEMVNKWERM-VSKEGQCELDVIPYLQTMTVDAISRTT 139
           IINPAFHLEK+K+M+PAFY CC E+V +WE++   KE   E+DV P+L  MT D IS T 
Sbjct: 153 IINPAFHLEKIKNMIPAFYHCCSEVVCQWEKLFTDKESPLEVDVWPWLVNMTADVISHTA 212

Query: 140 FGSSYEKGKKIFELQKQLNFSVIKYLSMMTNPIWRFLPTKSKNKMKKMGKEIKSLILGII 199
           FGSSY++G++IF+LQ +L   + +       P  RF PTKS  +MK + +E+  ++ GI+
Sbjct: 213 FGSSYKEGQRIFQLQGELAELIAQAFKKSYIPGSRFYPTKSNRRMKAIDREVDVILRGIV 272

Query: 200 NERQKAMEAGEAAYSDLLGILMESNLNEIRQHGNNKNVGMSIEDVIEECKLFYLAGQETT 259
           ++R+KA EAGE A  DLLGIL+ESN  E    GN    GMS+EDV++ECKLFY AGQETT
Sbjct: 273 SKREKAREAGEPANDDLLGILLESNSEE--SQGN----GMSVEDVMKECKLFYFAGQETT 332

Query: 260 ATLLIWTMILLSSYSEWQERARAEVREVF-DNRKPDYNGLSRLKIVTMILNEVLRLYPPV 319
           + LL+WTM+LLS + +WQ RAR EV +V  +N KPD   L+ LK++TMI NEVLRLYPPV
Sbjct: 333 SVLLVWTMVLLSHHQDWQARAREEVMQVLGENNKPDMESLNNLKVMTMIFNEVLRLYPPV 392

Query: 320 SVLDRVVEKEMKLGNLSLPAGVILRLPIIFIHHDRELWGEDALEFNPQRFFDGVSKATKN 379
           + L RVV KEMKLG L+LPAG+ + LP I +  D ELWG+DA +F P+RF DG+SKATKN
Sbjct: 393 AQLKRVVNKEMKLGELTLPAGIQIYLPTILVQRDTELWGDDAADFKPERFRDGLSKATKN 452

Query: 380 QTIFIPFGLGPRTCLGQNFAMIEAKMALSTILQQFSFQLSPSYTHAPFVTITTQPQHGAH 439
           Q  F PFG GPR C+GQNFAM+EAKMA++ ILQ+FSF+LSPSY HAP   +TT+PQ GAH
Sbjct: 453 QVSFFPFGWGPRICIGQNFAMLEAKMAMALILQKFSFELSPSYVHAPQTVMTTRPQFGAH 512

Query: 440 IILHKL 444
           +ILHKL
Sbjct: 513 LILHKL 512

[Close]

BLAST of MC11g_new0170 vs. TAIR 10
Match: AT3G14620.1 (cytochrome P450, family 72, subfamily A, polypeptide 8 )

HSP 1 Score: 484.6 bits (1246), Expect = 8.7e-137
Identity = 239/427 (55.97%), Postives = 316/427 (74.00%), Query Frame = 0


Query: 20  KNSFTWNGPIPSVLTMEPEQVKTAFMQVDDFRKPKMDHLIKLIADGLVNHEGPKWEKHRK 79
           K S+ W GPI SV+  +PE +K    +V DF KP +  +++L A G+  +EG KW KHRK
Sbjct: 98  KTSYMWMGPIASVIVTKPEHIKDVLNRVYDFPKPPVHPIVELFATGVALYEGEKWSKHRK 157

Query: 80  IINPAFHLEKLKDMVPAFYQCCDEMVNKWERMVSKEGQC-ELDVIPYLQTMTVDAISRTT 139
           IINP+FHLEKLK M+PAFY+ C EM++KWE++V+++G   E+DV PYL  +T D ISRT 
Sbjct: 158 IINPSFHLEKLKIMIPAFYESCSEMISKWEKLVTEQGSSNEIDVWPYLGDLTSDVISRTA 217

Query: 140 FGSSYEKGKKIFELQKQLNFSVIKYLSMMTNPIWRFLPTKSKNKMKKMGKEIKSLILGII 199
           FGSSYE+GK+IFELQ++    V+K L +   P  RFLPTK+  +M+++ KE+KS +  II
Sbjct: 218 FGSSYEEGKRIFELQEEQGRRVLKALELAFIPGMRFLPTKNNLRMRQINKEVKSRLREII 277

Query: 200 NERQKAMEAGEAAYSDLLGILMESNLNEIRQHGNNKNVGMSIEDVIEECKLFYLAGQETT 259
            +RQ+ M+ GEA  +DLLGIL+ES         N+ + GMSIEDV+EEC+LF+ AGQETT
Sbjct: 278 MKRQRGMDTGEAPKNDLLGILLES---------NSGDHGMSIEDVVEECRLFHFAGQETT 337

Query: 260 ATLLIWTMILLSSYSEWQERARAEVREVF-DNRKPDYNGLSRLKIVTMILNEVLRLYPPV 319
           A LL+WTMI+LS + +WQ++AR E+ +V   N KP+++ LSRLK ++MILNEVLRLYPP 
Sbjct: 338 AVLLVWTMIMLSHHQKWQDQAREEILKVIGKNNKPNFDALSRLKTMSMILNEVLRLYPPG 397

Query: 320 SVLDRVVEKEMKLG-NLSLPAGVILRLPIIFIHHDRELWGEDALEFNPQRFFDGVSKATK 379
            +L R VEKE KLG +++LP G  + +P++ +H D ELWGED  EFNP+RF DG+SKATK
Sbjct: 398 ILLGRTVEKETKLGEDMTLPGGAQVVIPVLMVHRDPELWGEDVHEFNPERFADGISKATK 457

Query: 380 NQTIFIPFGLGPRTCLGQNFAMIEAKMALSTILQQFSFQLSPSYTHAPFVTITTQPQHGA 439
           NQ  F+PFG GPR C GQNFA++EAKMAL  ILQ+FSF+LSPSYTHAP   +T  PQ GA
Sbjct: 458 NQVSFLPFGWGPRFCPGQNFALMEAKMALVLILQRFSFELSPSYTHAPHTVLTLHPQFGA 515

Query: 440 HIILHKL 444
            +I H L
Sbjct: 518 PLIFHML 515

The following BLAST results are available for this feature:

BLAST of MC11g_new0170 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
H2DH21	2.0e-146	58.82	Cytochrome P450 CYP72A219 OS=Panax ginseng OX=4054 PE=2 SV=1	[more]
A0A0S2IHL2	1.0e-145	58.73	Cytochrome P450 72A397 OS=Kalopanax septemlobus OX=228393 GN=CYP72A397 PE=1 SV=1	[more]
A0A481NR20	2.7e-143	57.88	Cytochrome P450 72A552 OS=Barbarea vulgaris OX=50459 GN=CYP72A552 PE=1 SV=1	[more]
Q9LUC8	2.5e-141	57.35	Cytochrome P450 72A13 OS=Arabidopsis thaliana OX=3702 GN=CYP72A13 PE=2 SV=1	[more]
Q9LUC5	5.7e-141	57.11	Cytochrome P450 72A15 OS=Arabidopsis thaliana OX=3702 GN=CYP72A15 PE=2 SV=1	[more]

BLAST of MC11g_new0170 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140103.1	5.33e-313	100.00	cytochrome P450 CYP72A219-like [Momordica charantia]	[more]
XP_022140083.1	8.91e-235	77.23	cytochrome P450 CYP72A219-like isoform X1 [Momordica charantia]	[more]
XP_022156576.1	1.73e-230	76.24	cytochrome P450 CYP72A219-like [Momordica charantia]	[more]
KAA0064317.1	1.47e-222	72.30	cytochrome P450 CYP72A219-like protein [Cucumis melo var. makuwa]	[more]
XP_023536197.1	1.71e-222	72.88	cytochrome P450 CYP72A219-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MC11g_new0170 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CH88	2.58e-313	100.00	cytochrome P450 CYP72A219-like OS=Momordica charantia OX=3673 GN=LOC111010835 PE...	[more]
A0A6J1CH71	4.31e-235	77.23	cytochrome P450 CYP72A219-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A6J1DVD0	8.38e-231	76.24	cytochrome P450 CYP72A219-like OS=Momordica charantia OX=3673 GN=LOC111023453 PE...	[more]
A0A5A7VBB4	7.11e-223	72.30	Cytochrome P450 CYP72A219-like protein OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3BT76	1.80e-222	72.30	cytochrome P450 CYP72A219-like OS=Cucumis melo OX=3656 GN=LOC103493471 PE=3 SV=1	[more]

BLAST of MC11g_new0170 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G14660.1	1.8e-142	57.35	cytochrome P450, family 72, subfamily A, polypeptide 13	[more]
AT3G14690.1	4.0e-142	57.11	cytochrome P450, family 72, subfamily A, polypeptide 15	[more]
AT3G14680.1	2.2e-140	55.76	cytochrome P450, family 72, subfamily A, polypeptide 14	[more]
AT3G14610.1	6.4e-140	56.57	cytochrome P450, family 72, subfamily A, polypeptide 7	[more]
AT3G14620.1	8.7e-137	55.97	cytochrome P450, family 72, subfamily A, polypeptide 8	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 391..402 score: 41.1 coord: 253..270 score: 40.41 coord: 382..391 score: 59.14 coord: 305..316 score: 40.16
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 25..419 e-value: 2.0E-82 score: 277.4
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 262..288 score: 24.87 coord: 391..414 score: 34.09 coord: 242..259 score: 25.64 coord: 381..391 score: 54.43 coord: 345..369 score: 23.64 coord: 129..147 score: 19.66
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 18..442 e-value: 7.5E-104 score: 349.7
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 15..441
None	No IPR available	PANTHER	PTHR24282:SF12	CYTOCHROME P450 72A11-RELATED	coord: 19..434
None	No IPR available	PANTHER	PTHR24282	CYTOCHROME P450 FAMILY MEMBER	coord: 19..434
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 384..393

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC11g_new0170.1	MC11g_new0170.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen