MC11g0942 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC11g0942
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionbeta-amyrin 11-oxidase-like
LocationMC11: 8012646 .. 8017432 (+)
RNA-Seq ExpressionMC11g0942
SyntenyMC11g0942
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTTGAGCAATTTTTGGGGCATTTTGGGAGCTGTTTTAGGGTTATATATCCTTGTGTTTGGGTTATTGAGGAAATTCAACCACCTATGGTATTTTCTTAGGTTGGAAAGGAAGATTTACGATACACTCCCTCCCGGGGATATGGGATGGCCTCTTATTGGCTCTTTATTAACGTTTTACAAAGCATATAAAATCGGCGGCAACCCTAACTATGTTATGGATGATCTCGTTTCTAGGTATCTCTCTTCAACTTCTTCCTCATCAAGAATAAGATTATGGATGAAAATAACTGGATATATATTATAATTGAACTTTTGATCAATTTAGGTTGAGTCTGGTATGCCTTCTAATTTAAATACTTCAAAAAATTTCATAACCCATTTTTTGGTAGAAGTGGTTTTAATTAAAATCATTGTGTTTGGTTACATTTTGTTCAAATGATCTTAATCACAAATTTGTTCGGTGATTAGGGATGTTTAAAAAAACCCTACAACCCGATAAATCCAACCCAAATCGTAAGGGTTTGGTTGGGTTAGATGAAGCATCGAATTAGGTTACCTTTAAAAAATCGGATCAATCCAATCCAAACTCAAATTTATGTTTATGTGTATACCAATTTTTTTAATTTTATATATATATATATATNATGTCATCGAGATAATTAAAAATTAAAAAAAAAACACAACCTGACAACCCAACCCGAAAATATTGCGTTGGGTTGGGTTCACCATCCAATTCAAACTCGACACTTCCTATATATGGTCACCTCAAAATTTTTGGTTGGTCCCAAAATTACTCATAACCTGATCCAACCCAACCCGTGAACACCCTACCTTACATTCATGAATGATTCTATAAATGTCAAATTACCATGAAGTGATAGTCACATCAATCACTCTAGAGAAGTAATTTTTATTTATTATCAAATAATAAAGGTTTTGAAAAACTATTTGAGACACCTCCTTTCTCAAACCGTCCAACGTTTTTTTAAAAGATGTGATGATTATTGGAATATTTTCCCAACTTTGATAGGTTTTTTCTTTTGTTTATATTTTTTAAATATGAAAAGAGAGTCATAGAGAACTTTTCTAGCTTCATATATGTTAAAAGAAACCATAAAAGGTGTGAAGAGAGTTCTTTTTCAACAAAAAAAAAAAAAAAGTTAAAAGTTTTCCGCGGTGGTTTGAATAGAGGGTTTCACCCATCAAAATCTAAGTAAAAAATGGACTCTCCTCTCCTTGTCACGATATCAAAGTCCAAAAATGAGCATCTGGTCTAAAATGGAAAAATCGATGGGCTAGAGCAAAAATATCACGTTAAGAATCCTACACTTCTCTACTATAAATTTTTGTAAGGTCTATTTTCTTAGGATCTTAATATTTTCCACCTTGATCAATTTAAACTTTTATTCGAGGCAGATATGGAAAAGTTGGGATGTACAAATGCCACTTGTATGGGACTCCAACAATCATTGTGACTAATCCAGAAGCTTGTCGACGCATATACTCAGACGACGAACACTTCAAATTCAATTATCCCAAGACGGTGAACATATTACTCGGGGATGGCTCTTTAACGAGGCTCAATCCCAAAATTTTACACCGATTTATTGCACCTCCCATAAATGGCTCTGAGCCATTGTCTCGCTATGTGGAATTCATTGAGAAGTTAATTGTGGAAGCATTGGAGGAATGGAGCAGCAGAAGAGAAATAGAGTTGGTGCATGAGTTGAGAGGCCTCACCTTCAAAATCATTACTCATGTCTTCGTTGGGACTGCTTTAACACACTTTCAAACTTCTGAAATTGGTAAATTATATGCCAAGATATCTCCTGTCATCATGTCCATTTTCTCTTACGACTTGCCCGGTTCTGCTTTTCGTCAAGCAGTCAAGGTATTAAGCTCGTTTGATCGTTTTTGGACTTTGATACCATATCAATATTGATGGACAATAAGAGGAGTAGTCCATGTCTATTATAAACCATTGTTAGGTCCCTTCTCTTTTTAATGTGGGATCCTTAACGGTTTGTTATATTTTGAAAAGTGCCTGTTCCCTTCGATAATGAAAAAAATGGACCTTACAATGATTTATAATAGATATGAGTTATGTCTTTCATTGCCAATTGATTTTGAGATGAAATCCATGAATATTAATAATGTTTATGCCAAAAGTATATCATTTATATTAATTAACTATAGTTTGTTCGGAGTGAAGATCATGTGTATATATGTGAGAGACAACATCTCTAAAGTTTTTTTACGTAAACATCAAAAGCAGAGTCATGAGGGTTCTTAAAATGGACAAATATATCACTGTTGAGAGTCACTTATCACTAACATACCCATGTTTCAATTTTTGCAATTGTATATCTTTCTCGAGTAGAGGATTTATAATTTTTTCATATATTATTATTTATAAACGCATATCACTGAAAGGCTCTACTGAGACAAAGATTTTGTGTATAACGTTGCAGGCAAGAGACGAGATATTGAACATCCTGGGGCCCGTGATAGCGGAGAGGAGAAGAGTTATTGAAAAGAATGAAGAAATGGAGGAGAAGTTTCAAGTTGATGTAGCTCTTAAGGCCAAGGATGCTAATGGGGAATTTTCTTTCAACAACAATGCAGTTGTATGCATGTTGCTTGGACTGCTGTTTGCTGGTCGTGATACATCAGCTCATGCTGCTATGTGGCTTCTCATATACGTATTACAACACCCTCAAGTCTACCAAAAGGCCAAGGTTGGTGTTAAGAGCCTAGCTTCGAGAATATATTAAATGATTAAATCACCCATTCTTGTCTCGAACAAAATGCAAGACTTTAGTTACTTAGTACCTGTTTGATAACGTTTTCATTTTTTTCTTGTTTCTTCTTTTTTCTAATAAAGAAAAATAGAATTAGGTGGCCCCAGCACCTATGGCTTCAAGAATATTTATTAAAAAAAAATTGAAAAATAAGGAACCTAGATAGTAGCAAAATGTTTAGGTTTCTTAATAAACTTAGAAACAAAAAACTTAAACATTACCCAACGAGCCATTATTGTTGTTTTCTTTCTTTTTTTTAAGAATTTTGATGATGTTTTTATTTCCGTTTTGGGTTTTTCAGGAAGAGCAAGAGATGATCCTAAAACAAAGACCATCTACTCAAAAGGGATTGATTTATAAAGAAATTAAACAAATGACATATGCTGCAAAGGTTTGTGTTTTTTTTTTTTTGTCTTAATTATGAATTTATATATATATATACAGTTTTTTACAAAAAAAAAATTCTAATTAAATAATTAAAAAAAAGGTTCAAATCATTTTATCTGACATGAGTGTTCTATATAGAAAAAAAATCCCAACTATTTGAAACGATTTATGTATTAACATAGTAGTACAAAACCGTAACAATGTTTTTCTTTTTGTTGAGTTGGGGACATGGACGAGATACCATATTAATTGGCAATAAGAAAAGTAGTTCACTTATATTATAAATCTTCGTTAGCCTTTTATCTTTTTAATATGGAATCTTTAACATGTTCCCTCAAGATTATAGCTCTTTTGACTCATCGATCTTGATCAAATTTTATTTTTAGATCCGATGATCATTTGAGCTTTGAGCTTCTTGAGAGATACAAGGGAATGAAAGTATCCCTAATTTTAGTAAAATACAATTCAAACTTGATTTTTCTTTTATTAATTTATTATTTCAGGTCATTAACGAGACGTTAAGAGTGAGTCATGTTACCACAGTAAGTTGTCGTAAAGCAACCACGGACATAAATATCCATGGTCAGTAATTATACTTGTATTTATTATTATGTATGCATATATATATATATGTCCATTTCTCTTTTAATTTTGTTGTTAAATTTTTGTGATTTTTTTTTATTATTAAGTTATATAATTACTTCATAGTAATTATTTACTTGTTGATAAATTTTTTCCCTCATTTTAGTTATCATTTTCTTTTTTCTTTGCTTATTTGTTTTAGGTTACACTATACCGAAAGAATGGAATGTAATAATTATGGTGAGAGACATTCATATGGATTCTCAAATATATCCAAATCCACAACAATTTGATCCTTCAAGATGGGATGTACGTTAAGATCCCCATCAATTCGATTTTCCTATTTTTATTTATTTATTTATTTATAAAATACATTTTTTTTTCTTGTGGGCAGAATTATACTCCAAAACCAGGGACGTTCCTGTCATTTGGGATGGGAGGTAGATTTTGTCCAGGAAATGAACTTGCAAGGCTTGAAATGACTATTTTACTTCACCATTTTCTCCTTAATTACAAGTAAGTTGCCTTTCATTTTTTGGTGAATTCAGAAAATATTTTTTTTTTTTTTTTTGAGATTTCTGCATATACAACCTTGTTGAGTAAAATATTTATTAAAACTATTTTCTGCTTAATTTGAATTTTTATTCTGGGCTAAATATACAAGATAGTCTATATTTTTAGGAAGAACATTATTCGAGTTGCGTTGTTCGTGTATCCAAATATGTTAACAAAACAATAATTTGAAAGATATATATCAATTATATTTGAAAATATGTGGGGGAGGGAGATTGAACTCACAGTCTACTGGTGGGAGACATATGTCAATTACCGTTAAACTATACTCGTTTTGACAACGAAAAATTGAAAGATTAAGTAGCCATTATAAATGATGATGCATATATAACAAATTTTTTTTTATTAATGTTGTAGATGTGAACGGCTCAATCCAGAAGCCCTAGTCTCCTCCCTGCCTATCCCTGTGCCTGTTGACAATTGCCTCGCCAAGTTTTCTAAG

mRNA sequence

ATTTTGAGCAATTTTTGGGGCATTTTGGGAGCTGTTTTAGGGTTATATATCCTTGTGTTTGGGTTATTGAGGAAATTCAACCACCTATGGTATTTTCTTAGGTTGGAAAGGAAGATTTACGATACACTCCCTCCCGGGGATATGGGATGGCCTCTTATTGGCTCTTTATTAACGTTTTACAAAGCATATAAAATCGGCGGCAACCCTAACTATGTTATGGATGATCTCGTTTCTAGATATGGAAAAGTTGGGATGTACAAATGCCACTTGTATGGGACTCCAACAATCATTGTGACTAATCCAGAAGCTTGTCGACGCATATACTCAGACGACGAACACTTCAAATTCAATTATCCCAAGACGGTGAACATATTACTCGGGGATGGCTCTTTAACGAGGCTCAATCCCAAAATTTTACACCGATTTATTGCACCTCCCATAAATGGCTCTGAGCCATTGTCTCGCTATGTGGAATTCATTGAGAAGTTAATTGTGGAAGCATTGGAGGAATGGAGCAGCAGAAGAGAAATAGAGTTGGTGCATGAGTTGAGAGGCCTCACCTTCAAAATCATTACTCATGTCTTCGTTGGGACTGCTTTAACACACTTTCAAACTTCTGAAATTGGTAAATTATATGCCAAGATATCTCCTGTCATCATGTCCATTTTCTCTTACGACTTGCCCGGTTCTGCTTTTCGTCAAGCAGTCAAGGCAAGAGACGAGATATTGAACATCCTGGGGCCCGTGATAGCGGAGAGGAGAAGAGTTATTGAAAAGAATGAAGAAATGGAGGAGAAGTTTCAAGTTGATGTAGCTCTTAAGGCCAAGGATGCTAATGGGGAATTTTCTTTCAACAACAATGCAGTTGTATGCATGTTGCTTGGACTGCTGTTTGCTGGTCGTGATACATCAGCTCATGCTGCTATGTGGCTTCTCATATACGTATTACAACACCCTCAAGTCTACCAAAAGGCCAAGGAAGAGCAAGAGATGATCCTAAAACAAAGACCATCTACTCAAAAGGGATTGATTTATAAAGAAATTAAACAAATGACATATGCTGCAAAGGTCATTAACGAGACGTTAAGAGTGAGTCATGTTACCACAGTAAGTTGTCGTAAAGCAACCACGGACATAAATATCCATGGTTACACTATACCGAAAGAATGGAATGTAATAATTATGGTGAGAGACATTCATATGGATTCTCAAATATATCCAAATCCACAACAATTTGATCCTTCAAGATGGGATAATTATACTCCAAAACCAGGGACGTTCCTGTCATTTGGGATGGGAGGTAGATTTTGTCCAGGAAATGAACTTGCAAGGCTTGAAATGACTATTTTACTTCACCATTTTCTCCTTAATTACAAATGTGAACGGCTCAATCCAGAAGCCCTAGTCTCCTCCCTGCCTATCCCTGTGCCTGTTGACAATTGCCTCGCCAAGTTTTCTAAG

Coding sequence (CDS)

ATTTTGAGCAATTTTTGGGGCATTTTGGGAGCTGTTTTAGGGTTATATATCCTTGTGTTTGGGTTATTGAGGAAATTCAACCACCTATGGTATTTTCTTAGGTTGGAAAGGAAGATTTACGATACACTCCCTCCCGGGGATATGGGATGGCCTCTTATTGGCTCTTTATTAACGTTTTACAAAGCATATAAAATCGGCGGCAACCCTAACTATGTTATGGATGATCTCGTTTCTAGATATGGAAAAGTTGGGATGTACAAATGCCACTTGTATGGGACTCCAACAATCATTGTGACTAATCCAGAAGCTTGTCGACGCATATACTCAGACGACGAACACTTCAAATTCAATTATCCCAAGACGGTGAACATATTACTCGGGGATGGCTCTTTAACGAGGCTCAATCCCAAAATTTTACACCGATTTATTGCACCTCCCATAAATGGCTCTGAGCCATTGTCTCGCTATGTGGAATTCATTGAGAAGTTAATTGTGGAAGCATTGGAGGAATGGAGCAGCAGAAGAGAAATAGAGTTGGTGCATGAGTTGAGAGGCCTCACCTTCAAAATCATTACTCATGTCTTCGTTGGGACTGCTTTAACACACTTTCAAACTTCTGAAATTGGTAAATTATATGCCAAGATATCTCCTGTCATCATGTCCATTTTCTCTTACGACTTGCCCGGTTCTGCTTTTCGTCAAGCAGTCAAGGCAAGAGACGAGATATTGAACATCCTGGGGCCCGTGATAGCGGAGAGGAGAAGAGTTATTGAAAAGAATGAAGAAATGGAGGAGAAGTTTCAAGTTGATGTAGCTCTTAAGGCCAAGGATGCTAATGGGGAATTTTCTTTCAACAACAATGCAGTTGTATGCATGTTGCTTGGACTGCTGTTTGCTGGTCGTGATACATCAGCTCATGCTGCTATGTGGCTTCTCATATACGTATTACAACACCCTCAAGTCTACCAAAAGGCCAAGGAAGAGCAAGAGATGATCCTAAAACAAAGACCATCTACTCAAAAGGGATTGATTTATAAAGAAATTAAACAAATGACATATGCTGCAAAGGTCATTAACGAGACGTTAAGAGTGAGTCATGTTACCACAGTAAGTTGTCGTAAAGCAACCACGGACATAAATATCCATGGTTACACTATACCGAAAGAATGGAATGTAATAATTATGGTGAGAGACATTCATATGGATTCTCAAATATATCCAAATCCACAACAATTTGATCCTTCAAGATGGGATAATTATACTCCAAAACCAGGGACGTTCCTGTCATTTGGGATGGGAGGTAGATTTTGTCCAGGAAATGAACTTGCAAGGCTTGAAATGACTATTTTACTTCACCATTTTCTCCTTAATTACAAATGTGAACGGCTCAATCCAGAAGCCCTAGTCTCCTCCCTGCCTATCCCTGTGCCTGTTGACAATTGCCTCGCCAAGTTTTCTAAG

Protein sequence

ILSNFWGILGAVLGLYILVFGLLRKFNHLWYFLRLERKIYDTLPPGDMGWPLIGSLLTFYKAYKIGGNPNYVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEHFKFNYPKTVNILLGDGSLTRLNPKILHRFIAPPINGSEPLSRYVEFIEKLIVEALEEWSSRREIELVHELRGLTFKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQAVKARDEILNILGPVIAERRRVIEKNEEMEEKFQVDVALKAKDANGEFSFNNNAVVCMLLGLLFAGRDTSAHAAMWLLIYVLQHPQVYQKAKEEQEMILKQRPSTQKGLIYKEIKQMTYAAKVINETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQFDPSRWDNYTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYKCERLNPEALVSSLPIPVPVDNCLAKFSK
Homology
BLAST of MC11g0942 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 1.7e-114
Identity = 216/490 (44.08%), Postives = 321/490 (65.51%), Query Frame = 0

Query: 6   WGILGAVLGLYILVFG--LLRKFNHLWYFLRLERKIYDTLPPGDMGWPLIGSLLTFYKAY 65
           W  + A   L   +FG   +R  N  +Y ++L RK +  LPPGDMGWPLIG LL+F K +
Sbjct: 5   WVCMSAATLLVCYIFGSKFVRNLNGWYYDVKLRRKEH-PLPPGDMGWPLIGDLLSFIKDF 64

Query: 66  KIGGNPNYVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEHFKFNYPKTVN 125
              G+P+  +++LV +YG+ G+YK HL+G P+IIV  P+ CRR+ +DD +FK  YPK++ 
Sbjct: 65  S-SGHPDSFINNLVLKYGRSGIYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYPKSIK 124

Query: 126 ILLGDG---SLTRLNPKILHRFIAPPINGSEPLSRYVEFIEKLIVEALEEWSS-RREIEL 185
            L        ++    ++  R I  PI G + L+ Y+E +E++++ +LEE SS +  +EL
Sbjct: 125 ELARCRPMIDVSNAEHRLFRRLITSPIVGHKALAMYLERLEEIVINSLEELSSMKHPVEL 184

Query: 186 VHELRGLTFKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQAVKAR 245
           + E++ ++FK I HVF+G++       +IG  +  +   + SI   ++PG  F +A++AR
Sbjct: 185 LKEMKKVSFKAIVHVFMGSSNQDI-IKKIGSSFTDLYNGMFSI-PINVPGFTFHKALEAR 244

Query: 246 DEILNILGPVIAERRRVIEK--NEEMEEKFQVDVALKAKDANGEFSFNNNAVVCMLLGLL 305
            ++  I+ PV+ ERR +IE    E  + K  +D+ L+ KD NG     +  +  +L+GLL
Sbjct: 245 KKLAKIVQPVVDERRLMIENGPQEGSQRKDLIDILLEVKDENGR-KLEDEDISDLLIGLL 304

Query: 306 FAGRDTSAHAAMWLLIYVLQHPQVYQKAKEEQEMILKQRPSTQKGLIYKEIKQMTYAAKV 365
           FAG +++A + MW + Y+ QHP + +KAKEEQE I + R S+QK L  KEIKQM Y ++V
Sbjct: 305 FAGHESTATSLMWSITYLTQHPHILKKAKEEQEEITRTRFSSQKQLSLKEIKQMVYLSQV 364

Query: 366 INETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQFDPSRW 425
           I+ETLR +++   + R+AT D+NI+GY IPK W V+I  R IHMDS+ YPNP++F+PSRW
Sbjct: 365 IDETLRCANIAFATFREATADVNINGYIIPKGWRVLIWARAIHMDSEYYPNPEEFNPSRW 424

Query: 426 DNYTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYKCERLNPEALVSSLPIPVP 485
           D+Y  K GTFL FG G R CPG +LA+LE++I LH+FL NY+ ER+NPE  V+SLP+  P
Sbjct: 425 DDYNAKAGTFLPFGAGSRLCPGADLAKLEISIFLHYFLRNYRLERINPECHVTSLPVSKP 484

Query: 486 VDNCLAKFSK 488
            DNCLAK  K
Sbjct: 485 TDNCLAKVIK 489

BLAST of MC11g0942 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 382.9 bits (982), Expect = 5.5e-105
Identity = 197/480 (41.04%), Postives = 305/480 (63.54%), Query Frame = 0

Query: 12  VLGLYILVFGLLRKFNHLWYFLRL-ERKIYDTLPPGDMGWPLIGSLLTFYKAYKIGGNPN 71
           +LGL++L + +L++ N   Y  +L E+K Y  LPPGD+GWP+IG++ +F +A+K   +P 
Sbjct: 15  ILGLFVLKW-VLKRVNVWIYVSKLGEKKHY--LPPGDLGWPVIGNMWSFLRAFKT-SDPE 74

Query: 72  YVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEHFKFNYPKTVNILLGDGS 131
             +   ++RYG+ G+YK H++G P ++VT PE CRR+ +DD+ F   +PK+   L+G  S
Sbjct: 75  SFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKS 134

Query: 132 LTRLN---PKILHRFIAPPINGSEPLSRYVEFIEKLIVEALEEWSSRREIELVHELRGLT 191
              ++    K L R  + P+NG E LS Y++FIE+ +   LE+WS   EIE +  LR LT
Sbjct: 135 FVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGEIEFLSHLRKLT 194

Query: 192 FKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQAVKARDEILNILG 251
           FK+I ++F+ +   H   S + + Y  ++  + ++   +LPG A+ +A+KAR +++    
Sbjct: 195 FKVIMYIFLSSESEHVMDS-LEREYTNLNYGVRAM-GINLPGFAYHRALKARKKLVAAFQ 254

Query: 252 PVIAERRRVIEKNEEMEEKFQVDVALKAKDANGEFSFNNNAVVCMLLGLLFAGRDTSAHA 311
            ++  RR   ++N     K  +D  +  KD NG    ++  ++ +LL  L AG ++S H 
Sbjct: 255 SIVTNRRNQRKQNISSNRKDMLDNLIDVKDENGRV-LDDEEIIDLLLMYLNAGHESSGHL 314

Query: 312 AMWLLIYVLQHPQVYQKAKEEQEMILKQRPSTQKGLIYKEIKQMTYAAKVINETLRVSHV 371
            MW  I + +HP + QKAKEEQE I+K+R   QK L  KE ++M Y ++VI+ETLRV   
Sbjct: 315 TMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVITF 374

Query: 372 TTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQFDPSRWDNYTPKPGTF 431
           +  + R+A +D+ + GY IPK W V+   R++H+D +IYP+P++FDPSRW+ YTPK GTF
Sbjct: 375 SLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTF 434

Query: 432 LSFGMGGRFCPGNELARLEMTILLHHFLLNYKCERLNPEALVSSLPIPVPVDNCLAKFSK 488
           L FG+G   CPGN+LA+LE++I LHHFLL Y+ ER NP   V  LP   P DNCLA+ ++
Sbjct: 435 LPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARITR 486

BLAST of MC11g0942 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 7.4e-102
Identity = 191/479 (39.87%), Postives = 304/479 (63.47%), Query Frame = 0

Query: 12  VLGLYILVFGLLRKFNHLWYFLRLERKIYDTLPPGDMGWPLIGSLLTFYKAYKIGGNPNY 71
           VLG + L + L+RK N +W +     +    LPPGD+GWP IG++L+F +A+K   +P+ 
Sbjct: 16  VLGCFGLNW-LVRKVN-VWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKT-SDPDS 75

Query: 72  VMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEHFKFNYPKTVNILLGDGSL 131
               L+ RYG  G+YK H++G P+IIVT  + CRR+ +DD+ FK  +P +   L+G  S 
Sbjct: 76  FTRTLIKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSF 135

Query: 132 TRLN---PKILHRFIAPPINGSEPLSRYVEFIEKLIVEALEEWSSRREIELVHELRGLTF 191
             ++    K L R  A P+NG E LS Y+ +IE+ ++  L++W+   E E +  LR LTF
Sbjct: 136 VGISFEEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGEFEFLTHLRKLTF 195

Query: 192 KIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQAVKARDEILNILGP 251
           +II ++F+ +   +   + + + Y  ++  + ++ + ++PG A+ +A+KAR  ++     
Sbjct: 196 RIIMYIFLSSESENVMDA-LEREYTALNYGVRAM-AVNIPGFAYHRALKARKTLVAAFQS 255

Query: 252 VIAERRRVIEKNEEMEEKFQVDVALKAKDANGEFSFNNNAVVCMLLGLLFAGRDTSAHAA 311
           ++ ERR   ++N    +K  +D  L  KD +G+ + ++  ++ +LL  L AG ++S H  
Sbjct: 256 IVTERRNQRKQNILSNKKDMLDNLLNVKDEDGK-TLDDEEIIDVLLMYLNAGHESSGHTI 315

Query: 312 MWLLIYVLQHPQVYQKAKEEQEMILKQRPSTQKGLIYKEIKQMTYAAKVINETLRVSHVT 371
           MW  +++ +HP+V Q+AK EQEMILK RP  QKGL  KE ++M + ++V++ETLRV   +
Sbjct: 316 MWATVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFS 375

Query: 372 TVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQFDPSRWDN-YTPKPGTF 431
             + R+A TD+ ++GY IPK W V+   RD+H+D +++P+P++FDP+RWDN + PK G F
Sbjct: 376 LTAFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAF 435

Query: 432 LSFGMGGRFCPGNELARLEMTILLHHFLLNYKCERLNPEALVSSLPIPVPVDNCLAKFS 487
           L FG G   CPGN+LA+LE++I LHHFLL Y+ +R NPE  V  LP   P DNCLA+ S
Sbjct: 436 LPFGAGSHLCPGNDLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTDNCLARIS 488

BLAST of MC11g0942 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 1.1e-97
Identity = 182/490 (37.14%), Postives = 300/490 (61.22%), Query Frame = 0

Query: 4   NFWGILGAVLGLYILVFGLLRKFNHLWYFLRLERKIYDTLPPGDMGWPLIGSLLTFYKAY 63
           + W +  AV+   +LV  ++R+ +       L  +    LPPG+MGWP++GS+  F +A+
Sbjct: 16  DLWVLAAAVVAGVVLVDAVVRRAHDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRAF 75

Query: 64  KIGGNPNYVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEHFKFNYPKTVN 123
           K  GNP+  +   + R+G+ G+Y+  ++ +PTI+   PEAC+++  DDE F   +PK   
Sbjct: 76  K-SGNPDAFIASFIRRFGRTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATV 135

Query: 124 ILLGDGSLTRL---NPKILHRFIAPPINGSEPLSRYVEFIEKLIVEALEEWSSRR--EIE 183
            L+G  S   +   + + + +  A PING + L+ Y+ FI++ +V +L  WSS    ++E
Sbjct: 136 TLIGPKSFVNMSYDDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVE 195

Query: 184 LVHELRGLTFKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQAVKA 243
            + ELR +TFKII  +F+  A        + + Y  ++   M   + +LPG A+ +A++A
Sbjct: 196 FLTELRRMTFKIIVQIFMSGA-DDATMEALERSYTDLN-YGMRAMAINLPGFAYYRALRA 255

Query: 244 RDEILNILGPVIAERRRVIEKNEEMEEKF-QVDVALKAKDANGEFSFNNNAVVCMLLGLL 303
           R +++++L  V+  RR    K  +       +D  ++A+D  G     ++ +V +L+  L
Sbjct: 256 RRKLVSVLQGVLDGRRAAAAKGFKRSGAMDMMDRLIEAEDERGR-RLADDEIVDVLIMYL 315

Query: 304 FAGRDTSAHAAMWLLIYVLQHPQVYQKAKEEQEMILKQRPSTQKGLIYKEIKQMTYAAKV 363
            AG ++S H  MW  +++ ++P ++ +AK EQE I++  P+TQ GL  ++ K+M + ++V
Sbjct: 316 NAGHESSGHITMWATVFLQENPDIFARAKAEQEEIMRSIPATQNGLTLRDFKKMHFLSQV 375

Query: 364 INETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQFDPSRW 423
           ++ETLR  +++ VS R+AT DI ++GY IPK W V +  R +HMD Q+YP+P+ F+PSRW
Sbjct: 376 VDETLRCVNISFVSFRQATRDIFVNGYLIPKGWKVQLWYRSVHMDDQVYPDPKMFNPSRW 435

Query: 424 DNYTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYKCERLNPEALVSSLPIPVP 483
           +   PK GTFL FG+G R CPGN+LA+LE+++ LHHFLL YK +R NP+  V  LP P P
Sbjct: 436 EGPPPKAGTFLPFGLGARLCPGNDLAKLEISVFLHHFLLGYKLKRANPKCRVRYLPHPRP 495

Query: 484 VDNCLAKFSK 488
           VDNCLA  +K
Sbjct: 496 VDNCLATITK 501

BLAST of MC11g0942 vs. ExPASy Swiss-Prot
Match: Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 1.5e-97
Identity = 185/492 (37.60%), Postives = 289/492 (58.74%), Query Frame = 0

Query: 5   FWGILGAVLGLYILVFGLLRKFNHLWYF-LRLERKIYDTLPPGDMGWPLIGSLLTFYKAY 64
           +W +   V  L ++      +  H WY+   L       LPPGDMGWPL+G +  F +A+
Sbjct: 6   WWAVAAVVAALAVVALDAAVRAAHAWYWTASLGAGRRGRLPPGDMGWPLVGGMWAFLRAF 65

Query: 65  KIGGNPNYVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEHFKFNYPKTVN 124
           K  G P+  +D    R+G+ G+Y+  ++ +PTI+ T PEAC+++  DD+ F   +PK   
Sbjct: 66  K-SGRPDSFIDSFARRFGRAGLYRAFMFSSPTIMATTPEACKQVLMDDDAFVTGWPKATV 125

Query: 125 ILLGDGSLTRL---NPKILHRFIAPPINGSEPLSRYVEFIEKLIVEALEEWSSR---REI 184
            L+G  S   +     + L +  A PING + L+ Y+ FI+  +V  L  WS R      
Sbjct: 126 ALIGPKSFVNMGYDEHRRLRKLTAAPINGFDALTSYLGFIDDTVVTTLRGWSERGGDGHF 185

Query: 185 ELVHELRGLTFKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQAVK 244
           E + ELR +TF+II  +F+G A      +E+ + Y +++   M   + DLPG A+ +A++
Sbjct: 186 EFLTELRRMTFRIIVQIFMGGA-DERTAAELERTYTELN-YGMRAMAIDLPGFAYHKAIR 245

Query: 245 ARDEILNILGPVIAERRRVIEKNE--EMEEKFQVDVALKAKDANGEFSFNNNAVVCMLLG 304
           AR  ++  L  V+ ERR    K           +D  +  +D  G     ++ ++ +L+ 
Sbjct: 246 ARRRLVAALQRVLDERRARGGKTAAGAAAPVDMMDRLIAVEDEGGR-RLQDDEIIDVLVM 305

Query: 305 LLFAGRDTSAHAAMWLLIYVLQHPQVYQKAKEEQEMILKQRPSTQKGLIYKEIKQMTYAA 364
            L AG ++S H  MW  +++ ++P++  KAK EQE I++  P  QKGL  ++ ++M Y +
Sbjct: 306 YLNAGHESSGHITMWATVFLQENPEILAKAKAEQEAIMRSIPPGQKGLTLRDFRKMAYLS 365

Query: 365 KVINETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQFDPS 424
           +V++ETLR  +++ VS R+AT D+ ++GY IPK W V +  R +HMD Q+YP+P++FDPS
Sbjct: 366 QVVDETLRFVNISFVSFRQATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPKKFDPS 425

Query: 425 RWDNYTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYKCERLNPEALVSSLPIP 484
           RW+   P+ GTFL FG+G R CPGN+LA+LE+++ LHHFLL YK  R NP   V  LP P
Sbjct: 426 RWEGPPPRAGTFLPFGLGTRLCPGNDLAKLEISVFLHHFLLGYKLTRKNPNCRVRYLPHP 485

Query: 485 VPVDNCLAKFSK 488
            PVDNCLAK ++
Sbjct: 486 RPVDNCLAKITR 493

BLAST of MC11g0942 vs. NCBI nr
Match: BBI21930.1 (cytochrome P450 monooxygenase [Momordica charantia])

HSP 1 Score: 640 bits (1650), Expect = 8.79e-226
Identity = 301/488 (61.68%), Postives = 387/488 (79.30%), Query Frame = 0

Query: 1   ILSNFWGILGAVLGLYILVFGLLRKFNHLWYFLRLERKIYDTLPPGDMGWPLIGSLLTFY 60
           IL+NFW +LGA +GLY  VFG LR FN LWYFLRL  KIY+ LPPG MGWPL+GS L+FY
Sbjct: 3   ILNNFWALLGASIGLYFFVFGFLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLSFY 62

Query: 61  KAYKIGGNPNYVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEHFKFNYPK 120
           KA+KIGG+PN +++ LVSRYG+V MYK HLYG+P IIVT+PE CRR+Y DDE FK +YPK
Sbjct: 63  KAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSYPK 122

Query: 121 TVNILLGDGSLTRLNPKILHRFIAPPINGSEPLSRYVEFIEKLIVEALEEWSS-RREIEL 180
           +VNIL GD SL +++ KI+HR +A PINGSE LS+Y+ FIE+ +V+ LEEW S +  +EL
Sbjct: 123 SVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPVEL 182

Query: 181 VHELRGLTFKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQAVKAR 240
           + E + LTFK+IT VF+G A T  + +++  LYA+I   I+SIF YDLPG ++R+A+KAR
Sbjct: 183 LLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALKAR 242

Query: 241 DEILNILGPVIAERRRVIEKNEEMEEKFQVDVALKAKDANGEFSFNNNAVVCMLLGLLFA 300
            ++ NI+ PVI  RRR++E+NEE+EE  Q+D  +KA D NGE  FN+NA++CMLLGLL+A
Sbjct: 243 SKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFNDNAIICMLLGLLYA 302

Query: 301 GRDTSAHAAMWLLIYVLQHPQVYQKAKEEQEMILKQRPSTQKGLIYKEIKQMTYAAKVIN 360
           G  TSAH A W +++V ++P VYQKAKEEQEMI+KQRPSTQKGL +KEIKQM YA KVIN
Sbjct: 303 GYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKVIN 362

Query: 361 ETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQFDPSRWDN 420
           E LR++++   + R ATTD+N++GY IPK W V I  R +HMD +IY NP +FDPSRWDN
Sbjct: 363 EMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRWDN 422

Query: 421 YTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYKCERLNPEALVSSLPIPVPVD 480
           Y PKPG F+ FG+G R+CPGNELARLE+ ILLHHFLL Y+ ER+NP+A +S++P+P PVD
Sbjct: 423 YVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYRWERVNPKAPLSNIPVPAPVD 482

Query: 481 NCLAKFSK 487
           NCL K +K
Sbjct: 483 NCLTKITK 490

BLAST of MC11g0942 vs. NCBI nr
Match: XP_022157134.1 (beta-amyrin 11-oxidase-like isoform X1 [Momordica charantia])

HSP 1 Score: 638 bits (1646), Expect = 3.56e-225
Identity = 301/488 (61.68%), Postives = 386/488 (79.10%), Query Frame = 0

Query: 1   ILSNFWGILGAVLGLYILVFGLLRKFNHLWYFLRLERKIYDTLPPGDMGWPLIGSLLTFY 60
           IL+NFW +LGA +GLY  VFG LR FN LWYFLRL  KIY+ LPPG MGWPL+GS L+FY
Sbjct: 3   ILNNFWALLGASIGLYFFVFGFLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLSFY 62

Query: 61  KAYKIGGNPNYVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEHFKFNYPK 120
           KA+KIGG+PN +++ LVSRYG+V MYK HLYG+P IIVT+PE CRR+Y DDE FK +YPK
Sbjct: 63  KAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSYPK 122

Query: 121 TVNILLGDGSLTRLNPKILHRFIAPPINGSEPLSRYVEFIEKLIVEALEEWSS-RREIEL 180
           +VNIL GD SL +++ KI+HR +A PINGSE LS+Y+ FIE+ +V+ LEEW S +  +EL
Sbjct: 123 SVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPVEL 182

Query: 181 VHELRGLTFKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQAVKAR 240
           + E + LTFK+IT VF+G A T  + +++  LYA+I   I+SIF YDLPG ++R+A+KAR
Sbjct: 183 LLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALKAR 242

Query: 241 DEILNILGPVIAERRRVIEKNEEMEEKFQVDVALKAKDANGEFSFNNNAVVCMLLGLLFA 300
            ++ NI+ PVI  RRR++E+NEE+EE  Q+D  +KA D NGE  FN+NA+ CMLLGLL+A
Sbjct: 243 SKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFNDNAIFCMLLGLLYA 302

Query: 301 GRDTSAHAAMWLLIYVLQHPQVYQKAKEEQEMILKQRPSTQKGLIYKEIKQMTYAAKVIN 360
           G  TSAH A W +++V ++P VYQKAKEEQEMI+KQRPSTQKGL +KEIKQM YA KVIN
Sbjct: 303 GYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKVIN 362

Query: 361 ETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQFDPSRWDN 420
           E LR++++   + R ATTD+N++GY IPK W V I  R +HMD +IY NP +FDPSRWDN
Sbjct: 363 EMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRWDN 422

Query: 421 YTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYKCERLNPEALVSSLPIPVPVD 480
           Y PKPG F+ FG+G R+CPGNELARLE+ ILLHHFLL Y+ ER+NP+A +S++P+P PVD
Sbjct: 423 YVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYRWERVNPKAPLSNIPVPAPVD 482

Query: 481 NCLAKFSK 487
           NCL K +K
Sbjct: 483 NCLTKITK 490

BLAST of MC11g0942 vs. NCBI nr
Match: XP_022157153.1 (beta-amyrin 11-oxidase-like [Momordica charantia] >BBI21929.1 cytochrome P450 monooxygenase [Momordica charantia])

HSP 1 Score: 630 bits (1624), Expect = 7.85e-222
Identity = 295/488 (60.45%), Postives = 381/488 (78.07%), Query Frame = 0

Query: 1   ILSNFWGILGAVLGLYILVFGLLRKFNHLWYFLRLERKIYDTLPPGDMGWPLIGSLLTFY 60
           +LSNF  ILGA+LGLYILVFG LRKFN +WY  +L + +Y+ LPPGDMGWPL+GS L+FY
Sbjct: 3   LLSNFGAILGALLGLYILVFGFLRKFNDIWYRFKLGKNVYNALPPGDMGWPLVGSSLSFY 62

Query: 61  KAYKIGGNPNYVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEHFKFNYPK 120
           +A+KIGG+PN VMD+L+SR+G+V MYK HLYG P IIVT+PE CRRIY DDEHFK NYPK
Sbjct: 63  QAFKIGGDPNAVMDNLISRFGRVRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPK 122

Query: 121 TVNILLGDGSLTRLNPKILHRFIAPPINGSEPLSRYVEFIEKLIVEALEEWSSRR-EIEL 180
           +V +L  DGSL +++ KI+HR +A PING E LSRYVEFIE  +VE LEEW S +  + L
Sbjct: 123 SVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPVPL 182

Query: 181 VHELRGLTFKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQAVKAR 240
           +HE + LTFKII  VF+G   T  + +E+  LYA+I P IMSIF Y+LPG ++R+A+KAR
Sbjct: 183 LHEFKRLTFKIIIGVFLGPVFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKAR 242

Query: 241 DEILNILGPVIAERRRVIEKNEEMEEKFQVDVALKAKDANGEFSFNNNAVVCMLLGLLFA 300
            ++ NI+ PVI  R++ +E N ++EE  Q+D  +KA D NGE  FN+NA++CMLLGLL+A
Sbjct: 243 IKVENIVRPVIQNRKKALENNVDLEETCQLDAVIKATDKNGERVFNDNAIICMLLGLLYA 302

Query: 301 GRDTSAHAAMWLLIYVLQHPQVYQKAKEEQEMILKQRPSTQKGLIYKEIKQMTYAAKVIN 360
           G  TSAH A W +++V ++P VYQKAKEEQEMI+KQRP  QKGL +KEIKQM Y AK+IN
Sbjct: 303 GYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPPNQKGLNFKEIKQMKYTAKIIN 362

Query: 361 ETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQFDPSRWDN 420
           E LR++++   + R AT D+N++GYTIPK W   + +R +HMD +IY NP +F+PSRWDN
Sbjct: 363 EVLRLNNIVFTNFRMATKDVNVNGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWDN 422

Query: 421 YTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYKCERLNPEALVSSLPIPVPVD 480
           YTPKPG ++ FG+G RFCPGNELA+LEMT+LLHHFLL Y+ ER+N +ALVS++PIP  VD
Sbjct: 423 YTPKPGEYIPFGIGSRFCPGNELAKLEMTVLLHHFLLGYRWERVNRKALVSNIPIPALVD 482

Query: 481 NCLAKFSK 487
            CL   +K
Sbjct: 483 ECLTNITK 490

BLAST of MC11g0942 vs. NCBI nr
Match: XP_022157141.1 (beta-amyrin 11-oxidase-like isoform X2 [Momordica charantia])

HSP 1 Score: 572 bits (1474), Expect = 8.30e-200
Identity = 269/441 (61.00%), Postives = 350/441 (79.37%), Query Frame = 0

Query: 48  MGWPLIGSLLTFYKAYKIGGNPNYVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRI 107
           MGWPL+GS L+FYKA+KIGG+PN +++ LVSRYG+V MYK HLYG+P IIVT+PE CRR+
Sbjct: 1   MGWPLLGSSLSFYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRV 60

Query: 108 YSDDEHFKFNYPKTVNILLGDGSLTRLNPKILHRFIAPPINGSEPLSRYVEFIEKLIVEA 167
           Y DDE FK +YPK+VNIL GD SL +++ KI+HR +A PINGSE LS+Y+ FIE+ +V+ 
Sbjct: 61  YLDDERFKLSYPKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKG 120

Query: 168 LEEWSS-RREIELVHELRGLTFKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYD 227
           LEEW S +  +EL+ E + LTFK+IT VF+G A T  + +++  LYA+I   I+SIF YD
Sbjct: 121 LEEWGSVKGPVELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYD 180

Query: 228 LPGSAFRQAVKARDEILNILGPVIAERRRVIEKNEEMEEKFQVDVALKAKDANGEFSFNN 287
           LPG ++R+A+KAR ++ NI+ PVI  RRR++E+NEE+EE  Q+D  +KA D NGE  FN+
Sbjct: 181 LPGFSYRRALKARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFND 240

Query: 288 NAVVCMLLGLLFAGRDTSAHAAMWLLIYVLQHPQVYQKAKEEQEMILKQRPSTQKGLIYK 347
           NA+ CMLLGLL+AG  TSAH A W +++V ++P VYQKAKEEQEMI+KQRPSTQKGL +K
Sbjct: 241 NAIFCMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFK 300

Query: 348 EIKQMTYAAKVINETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIY 407
           EIKQM YA KVINE LR++++   + R ATTD+N++GY IPK W V I  R +HMD +IY
Sbjct: 301 EIKQMKYATKVINEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIY 360

Query: 408 PNPQQFDPSRWDNYTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYKCERLNPE 467
            NP +FDPSRWDNY PKPG F+ FG+G R+CPGNELARLE+ ILLHHFLL Y+ ER+NP+
Sbjct: 361 QNPLEFDPSRWDNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYRWERVNPK 420

Query: 468 ALVSSLPIPVPVDNCLAKFSK 487
           A +S++P+P PVDNCL K +K
Sbjct: 421 APLSNIPVPAPVDNCLTKITK 441

BLAST of MC11g0942 vs. NCBI nr
Match: XP_022139739.1 (beta-amyrin 11-oxidase-like [Momordica charantia])

HSP 1 Score: 539 bits (1389), Expect = 1.26e-189
Identity = 268/268 (100.00%), Postives = 268/268 (100.00%), Query Frame = 0

Query: 220 MSIFSYDLPGSAFRQAVKARDEILNILGPVIAERRRVIEKNEEMEEKFQVDVALKAKDAN 279
           MSIFSYDLPGSAFRQAVKARDEILNILGPVIAERRRVIEKNEEMEEKFQVDVALKAKDAN
Sbjct: 1   MSIFSYDLPGSAFRQAVKARDEILNILGPVIAERRRVIEKNEEMEEKFQVDVALKAKDAN 60

Query: 280 GEFSFNNNAVVCMLLGLLFAGRDTSAHAAMWLLIYVLQHPQVYQKAKEEQEMILKQRPST 339
           GEFSFNNNAVVCMLLGLLFAGRDTSAHAAMWLLIYVLQHPQVYQKAKEEQEMILKQRPST
Sbjct: 61  GEFSFNNNAVVCMLLGLLFAGRDTSAHAAMWLLIYVLQHPQVYQKAKEEQEMILKQRPST 120

Query: 340 QKGLIYKEIKQMTYAAKVINETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDI 399
           QKGLIYKEIKQMTYAAKVINETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDI
Sbjct: 121 QKGLIYKEIKQMTYAAKVINETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDI 180

Query: 400 HMDSQIYPNPQQFDPSRWDNYTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYK 459
           HMDSQIYPNPQQFDPSRWDNYTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYK
Sbjct: 181 HMDSQIYPNPQQFDPSRWDNYTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYK 240

Query: 460 CERLNPEALVSSLPIPVPVDNCLAKFSK 487
           CERLNPEALVSSLPIPVPVDNCLAKFSK
Sbjct: 241 CERLNPEALVSSLPIPVPVDNCLAKFSK 268

BLAST of MC11g0942 vs. ExPASy TrEMBL
Match: A0A5K7U6M8 (Cytochrome P450 monooxygenase OS=Momordica charantia OX=3673 GN=McCYP88L8 PE=2 SV=1)

HSP 1 Score: 640 bits (1650), Expect = 4.25e-226
Identity = 301/488 (61.68%), Postives = 387/488 (79.30%), Query Frame = 0

Query: 1   ILSNFWGILGAVLGLYILVFGLLRKFNHLWYFLRLERKIYDTLPPGDMGWPLIGSLLTFY 60
           IL+NFW +LGA +GLY  VFG LR FN LWYFLRL  KIY+ LPPG MGWPL+GS L+FY
Sbjct: 3   ILNNFWALLGASIGLYFFVFGFLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLSFY 62

Query: 61  KAYKIGGNPNYVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEHFKFNYPK 120
           KA+KIGG+PN +++ LVSRYG+V MYK HLYG+P IIVT+PE CRR+Y DDE FK +YPK
Sbjct: 63  KAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSYPK 122

Query: 121 TVNILLGDGSLTRLNPKILHRFIAPPINGSEPLSRYVEFIEKLIVEALEEWSS-RREIEL 180
           +VNIL GD SL +++ KI+HR +A PINGSE LS+Y+ FIE+ +V+ LEEW S +  +EL
Sbjct: 123 SVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPVEL 182

Query: 181 VHELRGLTFKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQAVKAR 240
           + E + LTFK+IT VF+G A T  + +++  LYA+I   I+SIF YDLPG ++R+A+KAR
Sbjct: 183 LLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALKAR 242

Query: 241 DEILNILGPVIAERRRVIEKNEEMEEKFQVDVALKAKDANGEFSFNNNAVVCMLLGLLFA 300
            ++ NI+ PVI  RRR++E+NEE+EE  Q+D  +KA D NGE  FN+NA++CMLLGLL+A
Sbjct: 243 SKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFNDNAIICMLLGLLYA 302

Query: 301 GRDTSAHAAMWLLIYVLQHPQVYQKAKEEQEMILKQRPSTQKGLIYKEIKQMTYAAKVIN 360
           G  TSAH A W +++V ++P VYQKAKEEQEMI+KQRPSTQKGL +KEIKQM YA KVIN
Sbjct: 303 GYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKVIN 362

Query: 361 ETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQFDPSRWDN 420
           E LR++++   + R ATTD+N++GY IPK W V I  R +HMD +IY NP +FDPSRWDN
Sbjct: 363 EMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRWDN 422

Query: 421 YTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYKCERLNPEALVSSLPIPVPVD 480
           Y PKPG F+ FG+G R+CPGNELARLE+ ILLHHFLL Y+ ER+NP+A +S++P+P PVD
Sbjct: 423 YVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYRWERVNPKAPLSNIPVPAPVD 482

Query: 481 NCLAKFSK 487
           NCL K +K
Sbjct: 483 NCLTKITK 490

BLAST of MC11g0942 vs. ExPASy TrEMBL
Match: A0A6J1DVM6 (beta-amyrin 11-oxidase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111023928 PE=3 SV=1)

HSP 1 Score: 638 bits (1646), Expect = 1.72e-225
Identity = 301/488 (61.68%), Postives = 386/488 (79.10%), Query Frame = 0

Query: 1   ILSNFWGILGAVLGLYILVFGLLRKFNHLWYFLRLERKIYDTLPPGDMGWPLIGSLLTFY 60
           IL+NFW +LGA +GLY  VFG LR FN LWYFLRL  KIY+ LPPG MGWPL+GS L+FY
Sbjct: 3   ILNNFWALLGASIGLYFFVFGFLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLSFY 62

Query: 61  KAYKIGGNPNYVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEHFKFNYPK 120
           KA+KIGG+PN +++ LVSRYG+V MYK HLYG+P IIVT+PE CRR+Y DDE FK +YPK
Sbjct: 63  KAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSYPK 122

Query: 121 TVNILLGDGSLTRLNPKILHRFIAPPINGSEPLSRYVEFIEKLIVEALEEWSS-RREIEL 180
           +VNIL GD SL +++ KI+HR +A PINGSE LS+Y+ FIE+ +V+ LEEW S +  +EL
Sbjct: 123 SVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPVEL 182

Query: 181 VHELRGLTFKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQAVKAR 240
           + E + LTFK+IT VF+G A T  + +++  LYA+I   I+SIF YDLPG ++R+A+KAR
Sbjct: 183 LLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALKAR 242

Query: 241 DEILNILGPVIAERRRVIEKNEEMEEKFQVDVALKAKDANGEFSFNNNAVVCMLLGLLFA 300
            ++ NI+ PVI  RRR++E+NEE+EE  Q+D  +KA D NGE  FN+NA+ CMLLGLL+A
Sbjct: 243 SKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFNDNAIFCMLLGLLYA 302

Query: 301 GRDTSAHAAMWLLIYVLQHPQVYQKAKEEQEMILKQRPSTQKGLIYKEIKQMTYAAKVIN 360
           G  TSAH A W +++V ++P VYQKAKEEQEMI+KQRPSTQKGL +KEIKQM YA KVIN
Sbjct: 303 GYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKVIN 362

Query: 361 ETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQFDPSRWDN 420
           E LR++++   + R ATTD+N++GY IPK W V I  R +HMD +IY NP +FDPSRWDN
Sbjct: 363 EMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRWDN 422

Query: 421 YTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYKCERLNPEALVSSLPIPVPVD 480
           Y PKPG F+ FG+G R+CPGNELARLE+ ILLHHFLL Y+ ER+NP+A +S++P+P PVD
Sbjct: 423 YVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYRWERVNPKAPLSNIPVPAPVD 482

Query: 481 NCLAKFSK 487
           NCL K +K
Sbjct: 483 NCLTKITK 490

BLAST of MC11g0942 vs. ExPASy TrEMBL
Match: A0A5K7U6A4 (Cytochrome P450 monooxygenase OS=Momordica charantia OX=3673 GN=McCYP88L7 PE=2 SV=1)

HSP 1 Score: 630 bits (1624), Expect = 3.80e-222
Identity = 295/488 (60.45%), Postives = 381/488 (78.07%), Query Frame = 0

Query: 1   ILSNFWGILGAVLGLYILVFGLLRKFNHLWYFLRLERKIYDTLPPGDMGWPLIGSLLTFY 60
           +LSNF  ILGA+LGLYILVFG LRKFN +WY  +L + +Y+ LPPGDMGWPL+GS L+FY
Sbjct: 3   LLSNFGAILGALLGLYILVFGFLRKFNDIWYRFKLGKNVYNALPPGDMGWPLVGSSLSFY 62

Query: 61  KAYKIGGNPNYVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEHFKFNYPK 120
           +A+KIGG+PN VMD+L+SR+G+V MYK HLYG P IIVT+PE CRRIY DDEHFK NYPK
Sbjct: 63  QAFKIGGDPNAVMDNLISRFGRVRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPK 122

Query: 121 TVNILLGDGSLTRLNPKILHRFIAPPINGSEPLSRYVEFIEKLIVEALEEWSSRR-EIEL 180
           +V +L  DGSL +++ KI+HR +A PING E LSRYVEFIE  +VE LEEW S +  + L
Sbjct: 123 SVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPVPL 182

Query: 181 VHELRGLTFKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQAVKAR 240
           +HE + LTFKII  VF+G   T  + +E+  LYA+I P IMSIF Y+LPG ++R+A+KAR
Sbjct: 183 LHEFKRLTFKIIIGVFLGPVFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKAR 242

Query: 241 DEILNILGPVIAERRRVIEKNEEMEEKFQVDVALKAKDANGEFSFNNNAVVCMLLGLLFA 300
            ++ NI+ PVI  R++ +E N ++EE  Q+D  +KA D NGE  FN+NA++CMLLGLL+A
Sbjct: 243 IKVENIVRPVIQNRKKALENNVDLEETCQLDAVIKATDKNGERVFNDNAIICMLLGLLYA 302

Query: 301 GRDTSAHAAMWLLIYVLQHPQVYQKAKEEQEMILKQRPSTQKGLIYKEIKQMTYAAKVIN 360
           G  TSAH A W +++V ++P VYQKAKEEQEMI+KQRP  QKGL +KEIKQM Y AK+IN
Sbjct: 303 GYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPPNQKGLNFKEIKQMKYTAKIIN 362

Query: 361 ETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQFDPSRWDN 420
           E LR++++   + R AT D+N++GYTIPK W   + +R +HMD +IY NP +F+PSRWDN
Sbjct: 363 EVLRLNNIVFTNFRMATKDVNVNGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWDN 422

Query: 421 YTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYKCERLNPEALVSSLPIPVPVD 480
           YTPKPG ++ FG+G RFCPGNELA+LEMT+LLHHFLL Y+ ER+N +ALVS++PIP  VD
Sbjct: 423 YTPKPGEYIPFGIGSRFCPGNELAKLEMTVLLHHFLLGYRWERVNRKALVSNIPIPALVD 482

Query: 481 NCLAKFSK 487
            CL   +K
Sbjct: 483 ECLTNITK 490

BLAST of MC11g0942 vs. ExPASy TrEMBL
Match: A0A6J1DX36 (beta-amyrin 11-oxidase-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111023928 PE=3 SV=1)

HSP 1 Score: 572 bits (1474), Expect = 4.02e-200
Identity = 269/441 (61.00%), Postives = 350/441 (79.37%), Query Frame = 0

Query: 48  MGWPLIGSLLTFYKAYKIGGNPNYVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRI 107
           MGWPL+GS L+FYKA+KIGG+PN +++ LVSRYG+V MYK HLYG+P IIVT+PE CRR+
Sbjct: 1   MGWPLLGSSLSFYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRV 60

Query: 108 YSDDEHFKFNYPKTVNILLGDGSLTRLNPKILHRFIAPPINGSEPLSRYVEFIEKLIVEA 167
           Y DDE FK +YPK+VNIL GD SL +++ KI+HR +A PINGSE LS+Y+ FIE+ +V+ 
Sbjct: 61  YLDDERFKLSYPKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKG 120

Query: 168 LEEWSS-RREIELVHELRGLTFKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYD 227
           LEEW S +  +EL+ E + LTFK+IT VF+G A T  + +++  LYA+I   I+SIF YD
Sbjct: 121 LEEWGSVKGPVELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYD 180

Query: 228 LPGSAFRQAVKARDEILNILGPVIAERRRVIEKNEEMEEKFQVDVALKAKDANGEFSFNN 287
           LPG ++R+A+KAR ++ NI+ PVI  RRR++E+NEE+EE  Q+D  +KA D NGE  FN+
Sbjct: 181 LPGFSYRRALKARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFND 240

Query: 288 NAVVCMLLGLLFAGRDTSAHAAMWLLIYVLQHPQVYQKAKEEQEMILKQRPSTQKGLIYK 347
           NA+ CMLLGLL+AG  TSAH A W +++V ++P VYQKAKEEQEMI+KQRPSTQKGL +K
Sbjct: 241 NAIFCMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFK 300

Query: 348 EIKQMTYAAKVINETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIY 407
           EIKQM YA KVINE LR++++   + R ATTD+N++GY IPK W V I  R +HMD +IY
Sbjct: 301 EIKQMKYATKVINEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIY 360

Query: 408 PNPQQFDPSRWDNYTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYKCERLNPE 467
            NP +FDPSRWDNY PKPG F+ FG+G R+CPGNELARLE+ ILLHHFLL Y+ ER+NP+
Sbjct: 361 QNPLEFDPSRWDNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYRWERVNPK 420

Query: 468 ALVSSLPIPVPVDNCLAKFSK 487
           A +S++P+P PVDNCL K +K
Sbjct: 421 APLSNIPVPAPVDNCLTKITK 441

BLAST of MC11g0942 vs. ExPASy TrEMBL
Match: A0A6J1CDV4 (beta-amyrin 11-oxidase-like OS=Momordica charantia OX=3673 GN=LOC111010578 PE=3 SV=1)

HSP 1 Score: 539 bits (1389), Expect = 6.08e-190
Identity = 268/268 (100.00%), Postives = 268/268 (100.00%), Query Frame = 0

Query: 220 MSIFSYDLPGSAFRQAVKARDEILNILGPVIAERRRVIEKNEEMEEKFQVDVALKAKDAN 279
           MSIFSYDLPGSAFRQAVKARDEILNILGPVIAERRRVIEKNEEMEEKFQVDVALKAKDAN
Sbjct: 1   MSIFSYDLPGSAFRQAVKARDEILNILGPVIAERRRVIEKNEEMEEKFQVDVALKAKDAN 60

Query: 280 GEFSFNNNAVVCMLLGLLFAGRDTSAHAAMWLLIYVLQHPQVYQKAKEEQEMILKQRPST 339
           GEFSFNNNAVVCMLLGLLFAGRDTSAHAAMWLLIYVLQHPQVYQKAKEEQEMILKQRPST
Sbjct: 61  GEFSFNNNAVVCMLLGLLFAGRDTSAHAAMWLLIYVLQHPQVYQKAKEEQEMILKQRPST 120

Query: 340 QKGLIYKEIKQMTYAAKVINETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDI 399
           QKGLIYKEIKQMTYAAKVINETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDI
Sbjct: 121 QKGLIYKEIKQMTYAAKVINETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDI 180

Query: 400 HMDSQIYPNPQQFDPSRWDNYTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYK 459
           HMDSQIYPNPQQFDPSRWDNYTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYK
Sbjct: 181 HMDSQIYPNPQQFDPSRWDNYTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYK 240

Query: 460 CERLNPEALVSSLPIPVPVDNCLAKFSK 487
           CERLNPEALVSSLPIPVPVDNCLAKFSK
Sbjct: 241 CERLNPEALVSSLPIPVPVDNCLAKFSK 268

BLAST of MC11g0942 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 382.9 bits (982), Expect = 3.9e-106
Identity = 197/480 (41.04%), Postives = 305/480 (63.54%), Query Frame = 0

Query: 12  VLGLYILVFGLLRKFNHLWYFLRL-ERKIYDTLPPGDMGWPLIGSLLTFYKAYKIGGNPN 71
           +LGL++L + +L++ N   Y  +L E+K Y  LPPGD+GWP+IG++ +F +A+K   +P 
Sbjct: 15  ILGLFVLKW-VLKRVNVWIYVSKLGEKKHY--LPPGDLGWPVIGNMWSFLRAFKT-SDPE 74

Query: 72  YVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEHFKFNYPKTVNILLGDGS 131
             +   ++RYG+ G+YK H++G P ++VT PE CRR+ +DD+ F   +PK+   L+G  S
Sbjct: 75  SFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKS 134

Query: 132 LTRLN---PKILHRFIAPPINGSEPLSRYVEFIEKLIVEALEEWSSRREIELVHELRGLT 191
              ++    K L R  + P+NG E LS Y++FIE+ +   LE+WS   EIE +  LR LT
Sbjct: 135 FVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGEIEFLSHLRKLT 194

Query: 192 FKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQAVKARDEILNILG 251
           FK+I ++F+ +   H   S + + Y  ++  + ++   +LPG A+ +A+KAR +++    
Sbjct: 195 FKVIMYIFLSSESEHVMDS-LEREYTNLNYGVRAM-GINLPGFAYHRALKARKKLVAAFQ 254

Query: 252 PVIAERRRVIEKNEEMEEKFQVDVALKAKDANGEFSFNNNAVVCMLLGLLFAGRDTSAHA 311
            ++  RR   ++N     K  +D  +  KD NG    ++  ++ +LL  L AG ++S H 
Sbjct: 255 SIVTNRRNQRKQNISSNRKDMLDNLIDVKDENGRV-LDDEEIIDLLLMYLNAGHESSGHL 314

Query: 312 AMWLLIYVLQHPQVYQKAKEEQEMILKQRPSTQKGLIYKEIKQMTYAAKVINETLRVSHV 371
            MW  I + +HP + QKAKEEQE I+K+R   QK L  KE ++M Y ++VI+ETLRV   
Sbjct: 315 TMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVITF 374

Query: 372 TTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQFDPSRWDNYTPKPGTF 431
           +  + R+A +D+ + GY IPK W V+   R++H+D +IYP+P++FDPSRW+ YTPK GTF
Sbjct: 375 SLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTF 434

Query: 432 LSFGMGGRFCPGNELARLEMTILLHHFLLNYKCERLNPEALVSSLPIPVPVDNCLAKFSK 488
           L FG+G   CPGN+LA+LE++I LHHFLL Y+ ER NP   V  LP   P DNCLA+ ++
Sbjct: 435 LPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARITR 486

BLAST of MC11g0942 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 382.9 bits (982), Expect = 3.9e-106
Identity = 197/480 (41.04%), Postives = 305/480 (63.54%), Query Frame = 0

Query: 12  VLGLYILVFGLLRKFNHLWYFLRL-ERKIYDTLPPGDMGWPLIGSLLTFYKAYKIGGNPN 71
           +LGL++L + +L++ N   Y  +L E+K Y  LPPGD+GWP+IG++ +F +A+K   +P 
Sbjct: 15  ILGLFVLKW-VLKRVNVWIYVSKLGEKKHY--LPPGDLGWPVIGNMWSFLRAFKT-SDPE 74

Query: 72  YVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEHFKFNYPKTVNILLGDGS 131
             +   ++RYG+ G+YK H++G P ++VT PE CRR+ +DD+ F   +PK+   L+G  S
Sbjct: 75  SFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKS 134

Query: 132 LTRLN---PKILHRFIAPPINGSEPLSRYVEFIEKLIVEALEEWSSRREIELVHELRGLT 191
              ++    K L R  + P+NG E LS Y++FIE+ +   LE+WS   EIE +  LR LT
Sbjct: 135 FVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGEIEFLSHLRKLT 194

Query: 192 FKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQAVKARDEILNILG 251
           FK+I ++F+ +   H   S + + Y  ++  + ++   +LPG A+ +A+KAR +++    
Sbjct: 195 FKVIMYIFLSSESEHVMDS-LEREYTNLNYGVRAM-GINLPGFAYHRALKARKKLVAAFQ 254

Query: 252 PVIAERRRVIEKNEEMEEKFQVDVALKAKDANGEFSFNNNAVVCMLLGLLFAGRDTSAHA 311
            ++  RR   ++N     K  +D  +  KD NG    ++  ++ +LL  L AG ++S H 
Sbjct: 255 SIVTNRRNQRKQNISSNRKDMLDNLIDVKDENGRV-LDDEEIIDLLLMYLNAGHESSGHL 314

Query: 312 AMWLLIYVLQHPQVYQKAKEEQEMILKQRPSTQKGLIYKEIKQMTYAAKVINETLRVSHV 371
            MW  I + +HP + QKAKEEQE I+K+R   QK L  KE ++M Y ++VI+ETLRV   
Sbjct: 315 TMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVITF 374

Query: 372 TTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQFDPSRWDNYTPKPGTF 431
           +  + R+A +D+ + GY IPK W V+   R++H+D +IYP+P++FDPSRW+ YTPK GTF
Sbjct: 375 SLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTF 434

Query: 432 LSFGMGGRFCPGNELARLEMTILLHHFLLNYKCERLNPEALVSSLPIPVPVDNCLAKFSK 488
           L FG+G   CPGN+LA+LE++I LHHFLL Y+ ER NP   V  LP   P DNCLA+ ++
Sbjct: 435 LPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARITR 486

BLAST of MC11g0942 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 372.5 bits (955), Expect = 5.3e-103
Identity = 191/479 (39.87%), Postives = 304/479 (63.47%), Query Frame = 0

Query: 12  VLGLYILVFGLLRKFNHLWYFLRLERKIYDTLPPGDMGWPLIGSLLTFYKAYKIGGNPNY 71
           VLG + L + L+RK N +W +     +    LPPGD+GWP IG++L+F +A+K   +P+ 
Sbjct: 16  VLGCFGLNW-LVRKVN-VWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKT-SDPDS 75

Query: 72  VMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEHFKFNYPKTVNILLGDGSL 131
               L+ RYG  G+YK H++G P+IIVT  + CRR+ +DD+ FK  +P +   L+G  S 
Sbjct: 76  FTRTLIKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSF 135

Query: 132 TRLN---PKILHRFIAPPINGSEPLSRYVEFIEKLIVEALEEWSSRREIELVHELRGLTF 191
             ++    K L R  A P+NG E LS Y+ +IE+ ++  L++W+   E E +  LR LTF
Sbjct: 136 VGISFEEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGEFEFLTHLRKLTF 195

Query: 192 KIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQAVKARDEILNILGP 251
           +II ++F+ +   +   + + + Y  ++  + ++ + ++PG A+ +A+KAR  ++     
Sbjct: 196 RIIMYIFLSSESENVMDA-LEREYTALNYGVRAM-AVNIPGFAYHRALKARKTLVAAFQS 255

Query: 252 VIAERRRVIEKNEEMEEKFQVDVALKAKDANGEFSFNNNAVVCMLLGLLFAGRDTSAHAA 311
           ++ ERR   ++N    +K  +D  L  KD +G+ + ++  ++ +LL  L AG ++S H  
Sbjct: 256 IVTERRNQRKQNILSNKKDMLDNLLNVKDEDGK-TLDDEEIIDVLLMYLNAGHESSGHTI 315

Query: 312 MWLLIYVLQHPQVYQKAKEEQEMILKQRPSTQKGLIYKEIKQMTYAAKVINETLRVSHVT 371
           MW  +++ +HP+V Q+AK EQEMILK RP  QKGL  KE ++M + ++V++ETLRV   +
Sbjct: 316 MWATVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFS 375

Query: 372 TVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQFDPSRWDN-YTPKPGTF 431
             + R+A TD+ ++GY IPK W V+   RD+H+D +++P+P++FDP+RWDN + PK G F
Sbjct: 376 LTAFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAF 435

Query: 432 LSFGMGGRFCPGNELARLEMTILLHHFLLNYKCERLNPEALVSSLPIPVPVDNCLAKFS 487
           L FG G   CPGN+LA+LE++I LHHFLL Y+ +R NPE  V  LP   P DNCLA+ S
Sbjct: 436 LPFGAGSHLCPGNDLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTDNCLARIS 488

BLAST of MC11g0942 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 226.1 bits (575), Expect = 6.1e-59
Identity = 156/487 (32.03%), Postives = 244/487 (50.10%), Query Frame = 0

Query: 3   SNFWGILGA------VLGLYILVFGLLRKFNHLWYFLRLERKIYDTLPPGDMGWPLIGSL 62
           SNF+  L A       L + ++V  LL K+   W   RL       LPPG MG P IG  
Sbjct: 9   SNFFSSLYADEPALITLTIVVVVVVLLFKWWLHWKEQRLR------LPPGSMGLPYIGET 68

Query: 63  LTFYKAYKIGGNPNYVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEH-FK 122
           L  Y       NPN       ++YG +  +K H+ G P +++++PEA R +     H FK
Sbjct: 69  LRLYTE-----NPNSFFATRQNKYGDI--FKTHILGCPCVMISSPEAARMVLVSKAHLFK 128

Query: 123 FNYPKTVNILLGDGSLTRLNPKILHRFIAPPINGS---EPLSRYVEFIEKLIVEALEEWS 182
             YP +   ++G  +L   +    H  +   +  S     L   V  IE L+++ L  W+
Sbjct: 129 PTYPPSKERMIGPEALF-FHQGPYHSTLKRLVQSSFMPSALRPTVSHIELLVLQTLSSWT 188

Query: 183 SRREIELVHELRGLTFKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAF 242
           S++ I  +  ++   F +      G          I  LY ++     S+   DLPG+ F
Sbjct: 189 SQKSINTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSM-PLDLPGTLF 248

Query: 243 RQAVKARDEILNILGPVIAERRRVIEKNEE--MEEKFQVDVALKAKDANGEFSFNNNAVV 302
            +++KAR E+         E R+VIEK  E   EE   + V L AKD       +++ + 
Sbjct: 249 HKSMKARIELSE-------ELRKVIEKRRENGREEGGLLGVLLGAKDQKRN-GLSDSQIA 308

Query: 303 CMLLGLLFAGRDTSAHAAMWLLIYVLQHPQVYQKAKEEQEMILKQRPSTQKGLIYKEIKQ 362
             ++G++FA  DT+A    WLL Y+  HP + Q+   EQ  I ++     + + +++ ++
Sbjct: 309 DNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRK 368

Query: 363 MTYAAKVINETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQ 422
           M    +VI ETLR + V + + R+A  D+   GY IPK W V+ + R IH  S+ +P+P+
Sbjct: 369 MPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPE 428

Query: 423 QFDPSRWDNYTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYKCERLNPEALVS 478
           +FDPSR++   PKP T++ FG G   CPG+ELA+LEM ILLHH   +++ E +  E  + 
Sbjct: 429 KFDPSRFE-VAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEGIQ 471

BLAST of MC11g0942 vs. TAIR 10
Match: AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 226.1 bits (575), Expect = 6.1e-59
Identity = 156/487 (32.03%), Postives = 244/487 (50.10%), Query Frame = 0

Query: 3   SNFWGILGA------VLGLYILVFGLLRKFNHLWYFLRLERKIYDTLPPGDMGWPLIGSL 62
           SNF+  L A       L + ++V  LL K+   W   RL       LPPG MG P IG  
Sbjct: 9   SNFFSSLYADEPALITLTIVVVVVVLLFKWWLHWKEQRLR------LPPGSMGLPYIGET 68

Query: 63  LTFYKAYKIGGNPNYVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEH-FK 122
           L  Y       NPN       ++YG +  +K H+ G P +++++PEA R +     H FK
Sbjct: 69  LRLYTE-----NPNSFFATRQNKYGDI--FKTHILGCPCVMISSPEAARMVLVSKAHLFK 128

Query: 123 FNYPKTVNILLGDGSLTRLNPKILHRFIAPPINGS---EPLSRYVEFIEKLIVEALEEWS 182
             YP +   ++G  +L   +    H  +   +  S     L   V  IE L+++ L  W+
Sbjct: 129 PTYPPSKERMIGPEALF-FHQGPYHSTLKRLVQSSFMPSALRPTVSHIELLVLQTLSSWT 188

Query: 183 SRREIELVHELRGLTFKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAF 242
           S++ I  +  ++   F +      G          I  LY ++     S+   DLPG+ F
Sbjct: 189 SQKSINTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSM-PLDLPGTLF 248

Query: 243 RQAVKARDEILNILGPVIAERRRVIEKNEE--MEEKFQVDVALKAKDANGEFSFNNNAVV 302
            +++KAR E+         E R+VIEK  E   EE   + V L AKD       +++ + 
Sbjct: 249 HKSMKARIELSE-------ELRKVIEKRRENGREEGGLLGVLLGAKDQKRN-GLSDSQIA 308

Query: 303 CMLLGLLFAGRDTSAHAAMWLLIYVLQHPQVYQKAKEEQEMILKQRPSTQKGLIYKEIKQ 362
             ++G++FA  DT+A    WLL Y+  HP + Q+   EQ  I ++     + + +++ ++
Sbjct: 309 DNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRK 368

Query: 363 MTYAAKVINETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQ 422
           M    +VI ETLR + V + + R+A  D+   GY IPK W V+ + R IH  S+ +P+P+
Sbjct: 369 MPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPE 428

Query: 423 QFDPSRWDNYTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYKCERLNPEALVS 478
           +FDPSR++   PKP T++ FG G   CPG+ELA+LEM ILLHH   +++ E +  E  + 
Sbjct: 429 KFDPSRFE-VAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEGIQ 471

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
B5BSX11.7e-11444.08Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1[more]
Q9C5Y25.5e-10541.04Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2[more]
O230517.4e-10239.87Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1[more]
Q5VRM71.1e-9737.14Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ... [more]
Q9AXH91.5e-9737.60Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
BBI21930.18.79e-22661.68cytochrome P450 monooxygenase [Momordica charantia][more]
XP_022157134.13.56e-22561.68beta-amyrin 11-oxidase-like isoform X1 [Momordica charantia][more]
XP_022157153.17.85e-22260.45beta-amyrin 11-oxidase-like [Momordica charantia] >BBI21929.1 cytochrome P450 mo... [more]
XP_022157141.18.30e-20061.00beta-amyrin 11-oxidase-like isoform X2 [Momordica charantia][more]
XP_022139739.11.26e-189100.00beta-amyrin 11-oxidase-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A5K7U6M84.25e-22661.68Cytochrome P450 monooxygenase OS=Momordica charantia OX=3673 GN=McCYP88L8 PE=2 S... [more]
A0A6J1DVM61.72e-22561.68beta-amyrin 11-oxidase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A5K7U6A43.80e-22260.45Cytochrome P450 monooxygenase OS=Momordica charantia OX=3673 GN=McCYP88L7 PE=2 S... [more]
A0A6J1DX364.02e-20061.00beta-amyrin 11-oxidase-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1CDV46.08e-190100.00beta-amyrin 11-oxidase-like OS=Momordica charantia OX=3673 GN=LOC111010578 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT2G32440.13.9e-10641.04ent-kaurenoic acid hydroxylase 2 [more]
AT2G32440.23.9e-10641.04ent-kaurenoic acid hydroxylase 2 [more]
AT1G05160.15.3e-10339.87cytochrome P450, family 88, subfamily A, polypeptide 3 [more]
AT2G29090.16.1e-5932.03cytochrome P450, family 707, subfamily A, polypeptide 2 [more]
AT2G29090.26.1e-5932.03cytochrome P450, family 707, subfamily A, polypeptide 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002403Cytochrome P450, E-class, group IVPRINTSPR00465EP450IVcoord: 351..367
score: 31.22
coord: 290..316
score: 29.63
coord: 400..418
score: 34.82
coord: 437..455
score: 28.95
IPR001128Cytochrome P450PRINTSPR00385P450coord: 299..316
score: 28.44
coord: 356..367
score: 38.02
coord: 428..437
score: 50.2
coord: 437..448
score: 42.89
IPR001128Cytochrome P450PFAMPF00067p450coord: 44..461
e-value: 2.2E-68
score: 231.1
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 37..486
e-value: 3.3E-92
score: 311.6
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 43..477
NoneNo IPR availablePANTHERPTHR24286:SF289ENT-KAURENOIC ACID OXIDASE 2coord: 16..475
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 16..475
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 430..439

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC11g0942.1MC11g0942.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen