MC11g0443 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC11g0443
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionsynaptotagmin-4-like
LocationMC11: 3468450 .. 3472938 (+)
RNA-Seq ExpressionMC11g0443
SyntenyMC11g0443
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAAATCGTTGGGCGGATCCACGTGGCACAATTACGCAACGGTTAACTTGAAACCAAGTACCACGATTGCTCCTGTGAAATTCCGATCCACTGTATAACAAAGAGACGCGAAACCAAAGGAAAGAACCAATTCCTCTTGTTCTTGTTCTTGTTCTTCTTCGTCTTCTTCCGACGCCAAATTCCGATATGGAACAAATCTTCACTCATACATTCTCTTCTTCCGCCATTTCCGCATTGCAATTCCGTCCGCAGATTCCATTCGTCACTCGCCCGGAGCATGATCTCTGATTCCATTAACTATTGGCTTCTTCCTCTGTTTCGTTTACCTTTGGTTTCTCTCCCGCCATGTCGCTCTTTTTCGGTATCTTCATCGGCGTTGTTGTCGGAGTCCTACTGGTTATTGCTTTTGCTCGCGCCGGGAATGTTCGCGCCAAGCATCGCTCTGATTTGGTATCTCTTTATCTTTCTTCACTTTGCCCTTGGTTCAATATTATGATGCCCTGTTCTTGCACTTATTTTCTGTTCCATTGGTATTACTGGCGATTCAGGCGACGACAGTTGCGGCATTTGCAAGGATGACGGCACAGGATTCCAGAAAAATCCTTCCGAAGGAGTTTTATCCGTCGTGGGTTGTATTTACGAAGCAACAAAAGGTTGGTTCCGTTTTTTTCTTCTTTAATCAAACAGAAGATCTCGTTCCAAAATGGCAGTGGATTAATTTCAGGATTTAGAATTATAATTTCTTCATCGTTTGCAGTTAACTTGGCTCAATCACCAGCTTGATAGAATCTGGCCATTTGTTGACGCGGTAATCCAAATCTTCACTACTCTGATTCCTTAACTCATTGTTATGTACATTAGAACGTGCTTTTGATCCTCGAGTCGATTCGTCCTTTTCCTTCCTGTTATTATCTCCTGGGATTCCATGGAATGACGATTTCTGTAGGCAGCATCGGAGCTGATAAGGAGCAGTGTGGAGCCAATTCTCGAACAATTTCGACCGGTTGTATTATCTTCTTTGAAGTTCTCAAAGTTGACCCTTGGTACTATCGCTCCTCATTTTACAGGTAATAAATTGGTTCAATATTATCGATTAATTGAATGCATACACCAGATTCAGTACCAGTTATCACGACCTCCTTAATTAAAACAACGTAAATTCGTTCTATAGGAATCGCTGTACTCGAGGACGAAGCAGAAACTGATGGAATAACTTTAGAGTTGGAGATGCAGTGGGATGGTAATCCAAACATTGTTCTTGACATAAAAACTAAAGTTGGCGTTTCTTTACCAGTTCAGGTGATTAGGAGTTTCCTATATCTGACGGTGAAAAATAATTTAAGTGATAGACTTATCATGATTTTTGAATGATTACTTAAGTTTAAAAGCTCGTAAGCAGTTATGTAATTTCTTCTCCCTCGCCGAACTCTTCTCTTATTTCCTGACTTATTTATTTAGGTAAAAGATATTGCATTCACAGGGCTTTTCAGATTAATATTCAAGCCATTAGTTGATGAGTTTCCTTGTTTTGGAGCTGTGTGTTATTCCCTGAGGAAAAAGGTAAATTGTCAGGAAGATTATACATTTCTGAAGAGCTTTTTATGTGTAGTTCATTCAATACTTGTGCATCACAATAGTTCATGTTACTGCTGGCAGAAAAATCTGGATTTTAAACTTAAGATTGTTGGAGGGGACATATCCTCTATTCCTGGGGTTTCTGATGCTATTGAGGTAAATCAGACTTATTTGATCTTTCTGGCTCTTGTTGGCACATCAAATTTCTGTAGTATAAGCTAAACTAGTGGAATATTCTTGTCTAGGGAACAATTCGAGATGCCATCGAAGGTAGCATAACATGGCCGGTTCGAAATATTGTGCCCATCATAGCAGGGGATTATAGGTATGTTAAGCAACCAGAAAATAAAACATCAGTTCATGATCGTTTACCATTAGTTTTTTAACATGTGATGCTCAAGGTTAGCTAGTTTCAATCATATGTGGTTTCCTTTTATTATCGTAGCGACTTAGAGGCAAAGCCTGTTGGAACATTGGAAGTGAAGCTCGTGCAGGCAAAGGAATTAACCAATAAAGACATTATAGGAAAGTCGGATCCTTATGCTATGGTGTTTGTACGGCCATTGAAAGAAAGAACGAGAACAAGTAAAACCATTGTAAGACAAAGCTTCTCTTTTCGTTACATTTCTGGTCTTTAATGGCTGAACTGAGATAAGTTTTCCTTCTGCTGTATTCGGCCAGAATAACCAATTGAATCCCATATGGAATGAGCACTTTGATTTCGTAGTTGAAGATCCATCAACGCAGCACTTGACTATAAGAATTTTTGACGACGAAGGAGTTCAGGCATCTGAGCTAATTGGCTGTGCTCAAGTAGCATTAAAGGACCTCGAGCCTGGGAAAGTGAAGGACATTTGGTTGAAGCTGGTCAAAGATTTGGAAATTCAGAGGGATAACAAAAACAGGGGTCAGGTCAGAGCTCCAATGACATCAAGTTCTTCCTTTTGAAGTTATTTTGGGTTCAGTTAAAAGGAGTTATCTACCATGTAATTCACGAGTTTCTTCTCAAAACCAATTGAAAATGAGAGGGGTAGCTTATGTATCTTATAGACCTTGTTAGGTCCCTTGGTCTTTTCGACATGGGATTCTCAATGTCCACTCAATTGGTGGCTCTTTTGGGCTCACCAATCTTGGTTGGATCCTATTTTTCGATCGAATATTCGTATGAGCTTGTTGGGCTCTTATATTATATAATATTGAGTTCCATCAAAAAATCTATTGATAATTGGAAATGATAGGAGTAATCCATATATCTTATAAACTTTGTTAGTTCTTTTTGACGAGAGATCCTTAACATCTACTCAACTTATATCCATGCTTGACAGGTGCATTTGGAGCTTCTTTACTACCCCTACGGCACTGATCAGAGCCTCTATATGAACCCATTTAACCCAGATTATGCATTGACCTCGGTGGAGAAGGCTCTGAAGACAGTGAAGAGTGTGAACACGGTTACAAATGGCTCAGAAGATGCAGATTCAGTAAAACAAAGCTCCCCAAAGAAGAGGGACGTGATTGTAAGAGGAGTGTTGTCTGTGACAGTAATAGCTGCTGAAGATTTGCCGGCTGTAGATTTCATGGGAAAAGCCGATCCTTACGTCGTTCTCATTATGAAGAAATCCGACACTAAGGTTAAAACCAGGGTGAGATTCTCAGCTTTGTGTCGAAAGAAAGAATTAAAAATTTACACTCTAGTTTGCAGTTTTTCTTTTTCAACTTGATTGTTCACTGTCATATTAAGTAGACAAAACATCATCAGTATTCAATATCACCATTTCCTTGATGATATCAATTCGTCAATATTGTAATATCAAACTTCAAAATCTCATTGGACGATTTGATTCACTAGGTTGTGCATGACACCCTGAACCCAGTCTGGAATCAAACGTTTGACTTCCTGGTGGAGGATGCATTACACGATATGCTAATGGTGGAGGTCTGGGATCATGACACTTTTGGAAAGGTAATCTTCTCCATCTCAACTGTTTGAACTGAAATTAAGTGTAGAATTTGAACTGAAATTTTAGTTTGATTCATGAAGTCACCGAGGAAAGAGTTTGAGCTCGATGTGCCTATTAATACTGCCAATCGAAGCATTAGCTCACTATAAATTCCCTGAAGTTGAAGGTTCCTTCAGAAATGATTTTGCAGATGTCTATAGCTACCCTCTCGTACCACATTAAACTACAACTAAATCCAATAAACTATTAATCAGTTGAAAAATGATATTGTAGAGATCCAAACATAGTATCTCCGGTTCTCTTCTTACATCATACTACACTCAACTAAATTCAAAAAGAAAAACTATAGTACATTTGATCTTTTCAACACTTGTTTCATGTTTATACAGGATAAACTGGGGAGGGTCATAATGACACTGACAAGAGCGATACTGGAAGGGGAAGTTCAGGACTCTTATCCACTGGAAGGAGCCAAATCAGGGAGAATTTTTCTGCATCTCAAGTGGGCAGCTCAGCCAATATTCCGAGATACTTGACCAAAACCTTCTCATTCTTTTTTTCTCATCTCGTCTTACTCAGGTATCTGATTAAAACTTCGCGGCGGGAGGAAAGAATAATACTTACTTATTGACCACCATTAAAAATTTGATAACTATTTATTATTTTAAAAAAGGAAAATTATCTCTTCTCCCTCTCTGATTTATTTGTAATGGTGGTCAGGATGGGATGAAACTTATGTTTACGAGTAAGTATCTTCATATTCACCACAATTAAAAAATTGACAGCTAAAATAGATGAATGATAGCTGTCAATTTTTTAATAGCGATAAGTAGGAGATATTGCTGTTGGTATGCATCTAAGTTTTATTCATCGGTATTATTTGAGAGGGGAATTCATCACTTACGATCGACCACTCAAGCCAATTCTTTCCCTATTGTTTAAATAGATCAGTG

mRNA sequence

GGAAATCGTTGGGCGGATCCACGTGGCACAATTACGCAACGGTTAACTTGAAACCAAGTACCACGATTGCTCCTGTGAAATTCCGATCCACTGTATAACAAAGAGACGCGAAACCAAAGGAAAGAACCAATTCCTCTTGTTCTTGTTCTTGTTCTTCTTCGTCTTCTTCCGACGCCAAATTCCGATATGGAACAAATCTTCACTCATACATTCTCTTCTTCCGCCATTTCCGCATTGCAATTCCGTCCGCAGATTCCATTCGTCACTCGCCCGGAGCATGATCTCTGATTCCATTAACTATTGGCTTCTTCCTCTGTTTCGTTTACCTTTGGTTTCTCTCCCGCCATGTCGCTCTTTTTCGGTATCTTCATCGGCGTTGTTGTCGGAGTCCTACTGGTTATTGCTTTTGCTCGCGCCGGGAATGTTCGCGCCAAGCATCGCTCTGATTTGGCGACGACAGTTGCGGCATTTGCAAGGATGACGGCACAGGATTCCAGAAAAATCCTTCCGAAGGAGTTTTATCCGTCGTGGGTTGTATTTACGAAGCAACAAAAGTTAACTTGGCTCAATCACCAGCTTGATAGAATCTGGCCATTTGTTGACGCGGCAGCATCGGAGCTGATAAGGAGCAGTGTGGAGCCAATTCTCGAACAATTTCGACCGGTTGTATTATCTTCTTTGAAGTTCTCAAAGTTGACCCTTGGTACTATCGCTCCTCATTTTACAGGAATCGCTGTACTCGAGGACGAAGCAGAAACTGATGGAATAACTTTAGAGTTGGAGATGCAGTGGGATGGTAATCCAAACATTGTTCTTGACATAAAAACTAAAGTTGGCGTTTCTTTACCAGTTCAGGTAAAAGATATTGCATTCACAGGGCTTTTCAGATTAATATTCAAGCCATTAGTTGATGAGTTTCCTTGTTTTGGAGCTGTGTGTTATTCCCTGAGGAAAAAGAAAAATCTGGATTTTAAACTTAAGATTGTTGGAGGGGACATATCCTCTATTCCTGGGGTTTCTGATGCTATTGAGGGAACAATTCGAGATGCCATCGAAGGTAGCATAACATGGCCGGTTCGAAATATTGTGCCCATCATAGCAGGGGATTATAGCGACTTAGAGGCAAAGCCTGTTGGAACATTGGAAGTGAAGCTCGTGCAGGCAAAGGAATTAACCAATAAAGACATTATAGGAAAGTCGGATCCTTATGCTATGGTGTTTGTACGGCCATTGAAAGAAAGAACGAGAACAAGTAAAACCATTAATAACCAATTGAATCCCATATGGAATGAGCACTTTGATTTCGTAGTTGAAGATCCATCAACGCAGCACTTGACTATAAGAATTTTTGACGACGAAGGAGTTCAGGCATCTGAGCTAATTGGCTGTGCTCAAGTAGCATTAAAGGACCTCGAGCCTGGGAAAGTGAAGGACATTTGGTTGAAGCTGGTCAAAGATTTGGAAATTCAGAGGGATAACAAAAACAGGGGTCAGGTGCATTTGGAGCTTCTTTACTACCCCTACGGCACTGATCAGAGCCTCTATATGAACCCATTTAACCCAGATTATGCATTGACCTCGGTGGAGAAGGCTCTGAAGACAGTGAAGAGTGTGAACACGGTTACAAATGGCTCAGAAGATGCAGATTCAGTAAAACAAAGCTCCCCAAAGAAGAGGGACGTGATTGTAAGAGGAGTGTTGTCTGTGACAGTAATAGCTGCTGAAGATTTGCCGGCTGTAGATTTCATGGGAAAAGCCGATCCTTACGTCGTTCTCATTATGAAGAAATCCGACACTAAGGTTAAAACCAGGGTTGTGCATGACACCCTGAACCCAGTCTGGAATCAAACGTTTGACTTCCTGGTGGAGGATGCATTACACGATATGCTAATGGTGGAGGTCTGGGATCATGACACTTTTGGAAAGGATAAACTGGGGAGGGTCATAATGACACTGACAAGAGCGATACTGGAAGGGGAAGTTCAGGACTCTTATCCACTGGAAGGAGCCAAATCAGGGAGAATTTTTCTGCATCTCAAGTGGGCAGCTCAGCCAATATTCCGAGATACTTGACCAAAACCTTCTCATTCTTTTTTTCTCATCTCGTCTTACTCAGGTATCTGATTAAAACTTCGCGGCGGGAGGAAAGAATAATACTTACTTATTGACCACCATTAAAAATTTGATAACTATTTATTATTTTAAAAAAGGAAAATTATCTCTTCTCCCTCTCTGATTTATTTGTAATGGTGGTCAGGATGGGATGAAACTTATGTTTACGAGTAAGTATCTTCATATTCACCACAATTAAAAAATTGACAGCTAAAATAGATGAATGATAGCTGTCAATTTTTTAATAGCGATAAGTAGGAGATATTGCTGTTGGTATGCATCTAAGTTTTATTCATCGGTATTATTTGAGAGGGGAATTCATCACTTACGATCGACCACTCAAGCCAATTCTTTCCCTATTGTTTAAATAGATCAGTG

Coding sequence (CDS)

ATGTCGCTCTTTTTCGGTATCTTCATCGGCGTTGTTGTCGGAGTCCTACTGGTTATTGCTTTTGCTCGCGCCGGGAATGTTCGCGCCAAGCATCGCTCTGATTTGGCGACGACAGTTGCGGCATTTGCAAGGATGACGGCACAGGATTCCAGAAAAATCCTTCCGAAGGAGTTTTATCCGTCGTGGGTTGTATTTACGAAGCAACAAAAGTTAACTTGGCTCAATCACCAGCTTGATAGAATCTGGCCATTTGTTGACGCGGCAGCATCGGAGCTGATAAGGAGCAGTGTGGAGCCAATTCTCGAACAATTTCGACCGGTTGTATTATCTTCTTTGAAGTTCTCAAAGTTGACCCTTGGTACTATCGCTCCTCATTTTACAGGAATCGCTGTACTCGAGGACGAAGCAGAAACTGATGGAATAACTTTAGAGTTGGAGATGCAGTGGGATGGTAATCCAAACATTGTTCTTGACATAAAAACTAAAGTTGGCGTTTCTTTACCAGTTCAGGTAAAAGATATTGCATTCACAGGGCTTTTCAGATTAATATTCAAGCCATTAGTTGATGAGTTTCCTTGTTTTGGAGCTGTGTGTTATTCCCTGAGGAAAAAGAAAAATCTGGATTTTAAACTTAAGATTGTTGGAGGGGACATATCCTCTATTCCTGGGGTTTCTGATGCTATTGAGGGAACAATTCGAGATGCCATCGAAGGTAGCATAACATGGCCGGTTCGAAATATTGTGCCCATCATAGCAGGGGATTATAGCGACTTAGAGGCAAAGCCTGTTGGAACATTGGAAGTGAAGCTCGTGCAGGCAAAGGAATTAACCAATAAAGACATTATAGGAAAGTCGGATCCTTATGCTATGGTGTTTGTACGGCCATTGAAAGAAAGAACGAGAACAAGTAAAACCATTAATAACCAATTGAATCCCATATGGAATGAGCACTTTGATTTCGTAGTTGAAGATCCATCAACGCAGCACTTGACTATAAGAATTTTTGACGACGAAGGAGTTCAGGCATCTGAGCTAATTGGCTGTGCTCAAGTAGCATTAAAGGACCTCGAGCCTGGGAAAGTGAAGGACATTTGGTTGAAGCTGGTCAAAGATTTGGAAATTCAGAGGGATAACAAAAACAGGGGTCAGGTGCATTTGGAGCTTCTTTACTACCCCTACGGCACTGATCAGAGCCTCTATATGAACCCATTTAACCCAGATTATGCATTGACCTCGGTGGAGAAGGCTCTGAAGACAGTGAAGAGTGTGAACACGGTTACAAATGGCTCAGAAGATGCAGATTCAGTAAAACAAAGCTCCCCAAAGAAGAGGGACGTGATTGTAAGAGGAGTGTTGTCTGTGACAGTAATAGCTGCTGAAGATTTGCCGGCTGTAGATTTCATGGGAAAAGCCGATCCTTACGTCGTTCTCATTATGAAGAAATCCGACACTAAGGTTAAAACCAGGGTTGTGCATGACACCCTGAACCCAGTCTGGAATCAAACGTTTGACTTCCTGGTGGAGGATGCATTACACGATATGCTAATGGTGGAGGTCTGGGATCATGACACTTTTGGAAAGGATAAACTGGGGAGGGTCATAATGACACTGACAAGAGCGATACTGGAAGGGGAAGTTCAGGACTCTTATCCACTGGAAGGAGCCAAATCAGGGAGAATTTTTCTGCATCTCAAGTGGGCAGCTCAGCCAATATTCCGAGATACTTGA

Protein sequence

MSLFFGIFIGVVVGVLLVIAFARAGNVRAKHRSDLATTVAAFARMTAQDSRKILPKEFYPSWVVFTKQQKLTWLNHQLDRIWPFVDAAASELIRSSVEPILEQFRPVVLSSLKFSKLTLGTIAPHFTGIAVLEDEAETDGITLELEMQWDGNPNIVLDIKTKVGVSLPVQVKDIAFTGLFRLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIVGGDISSIPGVSDAIEGTIRDAIEGSITWPVRNIVPIIAGDYSDLEAKPVGTLEVKLVQAKELTNKDIIGKSDPYAMVFVRPLKERTRTSKTINNQLNPIWNEHFDFVVEDPSTQHLTIRIFDDEGVQASELIGCAQVALKDLEPGKVKDIWLKLVKDLEIQRDNKNRGQVHLELLYYPYGTDQSLYMNPFNPDYALTSVEKALKTVKSVNTVTNGSEDADSVKQSSPKKRDVIVRGVLSVTVIAAEDLPAVDFMGKADPYVVLIMKKSDTKVKTRVVHDTLNPVWNQTFDFLVEDALHDMLMVEVWDHDTFGKDKLGRVIMTLTRAILEGEVQDSYPLEGAKSGRIFLHLKWAAQPIFRDT
Homology
BLAST of MC11g0443 vs. ExPASy Swiss-Prot
Match: A0JJX5 (Synaptotagmin-4 OS=Arabidopsis thaliana OX=3702 GN=SYT4 PE=2 SV=1)

HSP 1 Score: 820.8 bits (2119), Expect = 9.3e-237
Identity = 397/574 (69.16%), Postives = 484/574 (84.32%), Query Frame = 0

Query: 1   MSLFFGIFIGVVVGVLLVIAFARAGNVRAKHRSDLATTVAAFARMTAQDSRKILPKEFYP 60
           M   FG+FIG+ V   LV+AFAR  +VR+  R+DLA T+AAFARMT QDSRK+LP +FYP
Sbjct: 1   MGFLFGLFIGIAVSFGLVVAFARYSSVRSTRRADLAKTIAAFARMTVQDSRKLLPGDFYP 60

Query: 61  SWVVFTKQQKLTWLNHQLDRIWPFVDAAASELIRSSVEPILEQFRPVVLSSLKFSKLTLG 120
           SWVVF+++QKL WLN +L++IWP+V+ AASELI+SSVEP+LEQ+ P +L+SLKFSK TLG
Sbjct: 61  SWVVFSQRQKLNWLNLELEKIWPYVNEAASELIKSSVEPVLEQYTPAMLASLKFSKFTLG 120

Query: 121 TIAPHFTGIAVLEDEAETDGITLELEMQWDGNPNIVLDIKTKVGVSLPVQVKDIAFTGLF 180
           T+AP FTG+++LE E+  +GIT+ELEMQWDGNP IVLD+KT +GVSLP++VK+I FTG+F
Sbjct: 121 TVAPQFTGVSILESESGPNGITMELEMQWDGNPKIVLDVKTLLGVSLPIEVKNIGFTGVF 180

Query: 181 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIVGGDISSIPGVSDAIEGTIRDAIEGSI 240
           RLIFKPLVDEFPCFGA+ YSLR+KK LDF LK++GG+++SIPG+SDAIE TIRDAIE SI
Sbjct: 181 RLIFKPLVDEFPCFGALSYSLREKKGLDFTLKVIGGELTSIPGISDAIEETIRDAIEDSI 240

Query: 241 TWPVRNIVPIIAGDYSDLEAKPVGTLEVKLVQAKELTNKDIIGKSDPYAMVFVRPLKERT 300
           TWPVR I+PI+ GDYSDLE KPVG L+VK+VQAK+L NKD+IGKSDPYA+VF+RPL +RT
Sbjct: 241 TWPVRKIIPILPGDYSDLELKPVGKLDVKVVQAKDLANKDMIGKSDPYAIVFIRPLPDRT 300

Query: 301 RTSKTINNQLNPIWNEHFDFVVEDPSTQHLTIRIFDDEGVQASELIGCAQVALKDLEPGK 360
           + +KTI+N LNPIWNEHF+F+VED STQHLT+R+FDDEGV +S+LIG AQV L +L PGK
Sbjct: 301 KKTKTISNSLNPIWNEHFEFIVEDVSTQHLTVRVFDDEGVGSSQLIGAAQVPLNELVPGK 360

Query: 361 VKDIWLKLVKDLEIQRDNKNRGQVHLELLYYPYGTDQSLYMNPFNPDYALTSVEKALKTV 420
           VKDIWLKLVKDLEIQRD KNRGQV LELLY P G +  L  NPFNPDY+LT +EK LK  
Sbjct: 361 VKDIWLKLVKDLEIQRDTKNRGQVQLELLYCPLGKEGGL-KNPFNPDYSLTILEKVLK-- 420

Query: 421 KSVNTVTNGSEDADSVKQSSPKKRDVIVRGVLSVTVIAAEDLPAVDFMGKADPYVVLIMK 480
                 +  S+  D  K  + KK+DVIVRGVLSVTV+AAEDLPAVDFMGKAD +VV+ +K
Sbjct: 421 ----PESEDSDATDMKKLVTSKKKDVIVRGVLSVTVVAAEDLPAVDFMGKADAFVVITLK 480

Query: 481 KSDTKVKTRVVHDTLNPVWNQTFDFLVEDALHDMLMVEVWDHDTFGKDKLGRVIMTLTRA 540
           KS+TK KTRVV D+LNPVWNQTFDF+VEDALHD+L +EVWDHD FGKDK+GRVIMTLTR 
Sbjct: 481 KSETKSKTRVVPDSLNPVWNQTFDFVVEDALHDLLTLEVWDHDKFGKDKIGRVIMTLTRV 540

Query: 541 ILEGEVQDSYPLEGAKSGRIFLHLKWAAQPIFRD 575
           +LEGE Q+ + L+GAKSG++ +HLKW  +   RD
Sbjct: 541 MLEGEFQEWFELDGAKSGKLCVHLKWTPRLKLRD 567

BLAST of MC11g0443 vs. ExPASy Swiss-Prot
Match: Q8L706 (Synaptotagmin-5 OS=Arabidopsis thaliana OX=3702 GN=SYT5 PE=2 SV=1)

HSP 1 Score: 810.1 bits (2091), Expect = 1.6e-233
Identity = 391/575 (68.00%), Postives = 482/575 (83.83%), Query Frame = 0

Query: 1   MSLFFGIFIGVVVGVLLVIAFARAGNVRAKHRSDLATTVAAFARMTAQDSRKILPKEFYP 60
           M    G+ IG++VG+ ++I F +  N R+K RS+LA TVAAFARMT +DSRK+LP EFYP
Sbjct: 1   MGFIVGVVIGLLVGIAIIIGFVKLENSRSKLRSELANTVAAFARMTVEDSRKLLPPEFYP 60

Query: 61  SWVVFTKQQKLTWLNHQLDRIWPFVDAAASELIRSSVEPILEQFRPVVLSSLKFSKLTLG 120
           SWVVF+++QKLTWLNH L +IWP+VD AASELI++SVEP+LEQ+RP +++SL FSKLTLG
Sbjct: 61  SWVVFSERQKLTWLNHHLTKIWPYVDEAASELIKASVEPVLEQYRPAIVASLTFSKLTLG 120

Query: 121 TIAPHFTGIAVLEDEAETDGITLELEMQWDGNPNIVLDIKTKVGVSLPVQVKDIAFTGLF 180
           T+AP FTG++V+  + + +GITLEL+MQWDGNPNIVL +KT VGVSLP+QVK+I FTG+F
Sbjct: 121 TVAPQFTGVSVI--DGDKNGITLELDMQWDGNPNIVLGVKTLVGVSLPIQVKNIGFTGVF 180

Query: 181 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIVGGDISSIPGVSDAIEGTIRDAIEGSI 240
           RLIF+PLV++FPCFGAV  SLR+KK LDF LK+VGGDIS+IPG+S+AIE TIRDA+E SI
Sbjct: 181 RLIFRPLVEDFPCFGAVSVSLREKKKLDFTLKVVGGDISAIPGLSEAIEETIRDAVEDSI 240

Query: 241 TWPVRNIVPIIAGDYSDLEAKPVGTLEVKLVQAKELTNKDIIGKSDPYAMVFVRPLKERT 300
           TWPVR ++PII GDYSDLE KPVG LEVKLVQAK LTNKD++GKSDP+A +F+RPL+E+T
Sbjct: 241 TWPVRKVIPIIPGDYSDLELKPVGMLEVKLVQAKNLTNKDLVGKSDPFAKMFIRPLREKT 300

Query: 301 RTSKTINNQLNPIWNEHFDFVVEDPSTQHLTIRIFDDEGVQASELIGCAQVALKDLEPGK 360
           + SKTINN LNPIWNEHF+FVVED STQHL +RI+DDEGVQASELIGCAQ+ L +LEPGK
Sbjct: 301 KRSKTINNDLNPIWNEHFEFVVEDASTQHLVVRIYDDEGVQASELIGCAQIRLCELEPGK 360

Query: 361 VKDIWLKLVKDLEIQRDNKNRGQVHLELLYYPYGTDQSLYMNPFNPDYALTSVEKALKTV 420
           VKD+WLKLVKDLEIQRD KNRG+VHLELLY PYG+   + +NPF    ++TS+E+ LK  
Sbjct: 361 VKDVWLKLVKDLEIQRDTKNRGEVHLELLYIPYGSGNGI-VNPFVTS-SMTSLERVLK-- 420

Query: 421 KSVNTVTNGSEDADSVKQSSPKKRDVIVRGVLSVTVIAAEDLPAVDFMGKADPYVVLIMK 480
                    ++  D    SS K++DVIVRGVLSVTVI+AE++P  D MGKADPYVVL MK
Sbjct: 421 ---------NDTTDEENASSRKRKDVIVRGVLSVTVISAEEIPIQDLMGKADPYVVLSMK 480

Query: 481 KSDTKVKTRVVHDTLNPVWNQTFDFLVEDALHDMLMVEVWDHDTFGKDKLGRVIMTLTRA 540
           KS  K KTRVV+D+LNPVWNQTFDF+VED LHDML++EVWDHDTFGKD +GR I+TLTR 
Sbjct: 481 KSGAKSKTRVVNDSLNPVWNQTFDFVVEDGLHDMLVLEVWDHDTFGKDYIGRCILTLTRV 540

Query: 541 ILEGEVQDSYPLEGAKSGRIFLHLKWAAQPIFRDT 576
           I+E E +D YPL+ +K+G++ LHLKW AQ I+RD+
Sbjct: 541 IMEEEYKDWYPLDESKTGKLQLHLKWMAQSIYRDS 560

BLAST of MC11g0443 vs. ExPASy Swiss-Prot
Match: Q9LEX1 (Calcium-dependent lipid-binding protein OS=Arabidopsis thaliana OX=3702 GN=CLB PE=1 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 1.7e-76
Identity = 158/406 (38.92%), Postives = 248/406 (61.08%), Query Frame = 0

Query: 1   MSLFFGIFIGVVVGVLLVIAFARAGNVRAKHRSDLATTVAAFARMTAQDSRKILPKEFYP 60
           M L  GI  G++ GV L+  ++R    R+  R   A  +     ++  D +KI    F P
Sbjct: 1   MGLISGILFGIIFGVALMAGWSRMMTHRSSKRVAKAVDMKLLGSLSRDDLKKICGDNF-P 60

Query: 61  SWVVFTKQQKLTWLNHQLDRIWPFVDAAASELIRSSVEPILEQFRPVVLSSLKFSKLTLG 120
            W+ F   +++ WLN  L ++WP++  AA+ +IR SVEP+LE +RP  ++SLKFSKLTLG
Sbjct: 61  QWISFPAFEQVKWLNKLLSKMWPYIAEAATMVIRDSVEPLLEDYRPPGITSLKFSKLTLG 120

Query: 121 TIAPHFTGIAVLEDEAETDGITLELEMQWDGNPNIVLDIKTKVGVSLPVQVKDIAFTGLF 180
            +AP   GI V     +   +T++++++W G+PNIVL + T +  S+P+Q+KD+    + 
Sbjct: 121 NVAPKIEGIRV--QSFKEGQVTMDVDLRWGGDPNIVLGV-TALVASIPIQLKDLQVFTVA 180

Query: 181 RLIFKPLVDEFPCFGAVCYSL--RKKKNLDFKLKIVGGDISSIPGVSDAIEGTIRDAIEG 240
           R+IF+ L DE PC  AV  +L    K  +D+ LK VGG +++IPG+SD I+ T+   ++ 
Sbjct: 181 RVIFQ-LADEIPCISAVVVALLAEPKPRIDYTLKAVGGSLTAIPGLSDMIDDTVDTIVKD 240

Query: 241 SITWPVRNIVPI--IAGDYSDLEAKPVGTLEVKLVQAKELTNKDIIGKSDPYAMVFVRPL 300
            + WP R +VPI  I  D SDLE KP G L V +V+A  L NK++IGKSDPYA +++RP+
Sbjct: 241 MLQWPHRIVVPIGGIPVDLSDLELKPQGKLIVTVVKATNLKNKELIGKSDPYATIYIRPV 300

Query: 301 -KERTRTSKTINNQLNPIWNEHFDFVVEDPSTQHLTIRIFDDEGVQASELIGCAQVALKD 360
            K +T   K I N LNP+W++ F+ + ED  TQ LT+ +FD + V   E +G  ++ L  
Sbjct: 301 FKYKT---KAIENNLNPVWDQTFELIAEDKETQSLTVEVFDKD-VGQDERLGLVKLPLSS 360

Query: 361 LEPGKVKDIWLKLVKDLEI--QRDNKNRGQVHLELLYYPYGTDQSL 400
           LE G  K++ L L+  L+    +D K+RG + L++ Y+ +  ++ +
Sbjct: 361 LEAGVTKELELNLLSSLDTLKVKDKKDRGSITLKVHYHEFNKEEQM 397

BLAST of MC11g0443 vs. ExPASy Swiss-Prot
Match: Q7XA06 (Synaptotagmin-3 OS=Arabidopsis thaliana OX=3702 GN=SYT3 PE=2 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 3.7e-68
Identity = 167/570 (29.30%), Postives = 293/570 (51.40%), Query Frame = 0

Query: 6   GIFIGVVVGVLLVIAFARAGNVRAKHRSDLATTVAAFARMTAQDSRKILPKEFYPSWVVF 65
           GI IG+++G  ++I    +       R  + T+++            +LP    P W+  
Sbjct: 16  GIPIGLILGFFVLIYSQPSHQEYPPARPLVETSISVLL--------DLLPD--IPLWMKN 75

Query: 66  TKQQKLTWLNHQLDRIWPFVDAAASELIRSSVEPILEQF-RPVVLSSLKFSKLTLGTIAP 125
              +++ W N  +  +WP++D A   +IRSSV+P+   +     + S++F  L+LGT+ P
Sbjct: 76  PDYERVDWFNKFISYMWPYLDKAVCGIIRSSVQPLFADYIGTFCIESIEFENLSLGTLPP 135

Query: 126 HFTGIAVLEDEAETDGITLELEMQWDGNPNIVLDIKTKVGVSLPVQVKDIAFTGLFRLIF 185
              G+   E   +   +  E  ++W GNPNIVL +K  + + + VQ+ D+ F  + R+  
Sbjct: 136 TVHGVKFYETNEKE--LLFEPSIKWAGNPNIVLVLKV-LSLRIRVQLVDLQFFAIVRVAL 195

Query: 186 KPLVDEFPCFGAVCYSLRKKKNLDFKLKIVGGDISSIPGVSDAIEGTIRDAIEGSITWPV 245
           KPL+  FPCFG V  SL +K ++DF LK++GGD+ SIPG+   ++ TI+  +     WP 
Sbjct: 196 KPLLPTFPCFGMVVVSLMEKPHVDFGLKVLGGDLMSIPGLYRYVQETIKRQVSSMYHWPQ 255

Query: 246 RNIVPIIAGDYSDLEAKPVGTLEVKLVQAKELTNKDIIGKSDPYAMVFVRPLKERTRTSK 305
              +PI+    + ++ KPVG L V +++A+ L  KD++G SDPY  + +   K   + + 
Sbjct: 256 VLEIPILDSSTASVK-KPVGLLHVSILRARNLLKKDLLGTSDPYVKLSLTGEKLPAKKTT 315

Query: 306 TINNQLNPIWNEHFDFVVEDPSTQHLTIRIFDDEGVQASELIGCAQVALKDLEPGKVKDI 365
                LNP WNEHF  +V+DP++Q L + +FD + V   + +G   + L+ + PG+ K+ 
Sbjct: 316 IKKRNLNPEWNEHFKLIVKDPNSQVLQLEVFDWDKVGGHDRLGMQMIPLQKINPGERKEF 375

Query: 366 WLKLVKDLEI---QRDNKNRGQVHLELLYYPYGTDQSLYMNPFNPDYALTSVEKALKTVK 425
            L L+K+  +     D K RG++ ++L Y P+                    E+++K  K
Sbjct: 376 NLDLIKNSNVVMDSGDKKKRGRLEVDLRYVPFR-------------------EESIKRRK 435

Query: 426 SVNTVTNGSEDADSVKQSSPKKRDVIVRGVLSVTVIAAEDLPAVDFMGKADPYVVLIMKK 485
             +     SED D + Q+          G+LSV V +A+D+        ++PY V++ + 
Sbjct: 436 E-SREEKSSEDDDFLSQA----------GLLSVAVQSAKDVEGK--KKHSNPYAVVLFR- 495

Query: 486 SDTKVKTRVVHDTLNPVWNQTFDFLVED-ALHDMLMVEVWDHDT----FGKDKLGRVIMT 545
              K KT+++  T +P WN+ F F +E+  + + + VEV    T      K++LG V + 
Sbjct: 496 -GEKKKTKMLKKTRDPRWNEEFQFTLEEPPVKESIRVEVMSKGTGFHFRSKEELGHVDIN 537

Query: 546 LTRAILEGEVQDSYPLEGAKSGRIFLHLKW 567
           L   +  G +   Y L  +++G I + ++W
Sbjct: 556 LDDVVDNGRINQKYHLINSRNGIIHIEIRW 537

BLAST of MC11g0443 vs. ExPASy Swiss-Prot
Match: B6ETT4 (Synaptotagmin-2 OS=Arabidopsis thaliana OX=3702 GN=SYT2 PE=2 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 6.6e-65
Identity = 181/572 (31.64%), Postives = 286/572 (50.00%), Query Frame = 0

Query: 5   FGIFIGVVVGVLLVIAFARAGNVRAKHRSDLA-TTVAAFARMTAQDSRKILPKEFYPSWV 64
           FG  IG+V+G  L I F           +D+    +     + ++    + P+   P WV
Sbjct: 15  FGTTIGIVIGYYLFIYF---------QSTDVEDPEIKPLVELDSETIATMFPE--IPMWV 74

Query: 65  VFTKQQKLTWLNHQLDRIWPFVDAAASELIRSSVEPIL-EQFRPVVLSSLKFSKLTLGTI 124
                 ++ WLN  +  +WP++D A  ++ +S  +PI+ EQ     + S++F  LTLG++
Sbjct: 75  KNPDFDRIDWLNKLIGHMWPYMDKAICKMAKSIAKPIIAEQIPNYKIDSVEFEMLTLGSL 134

Query: 125 APHFTGIAVLEDEAETDGITLELEMQWDGNPNIVLDIKTKVGVSLPVQVKDIAFTGLFRL 184
            P F G+ V   + +   I +EL ++W GNPNI++  K   G+   VQV D+      R+
Sbjct: 135 PPSFQGMKVYATDDKE--IIMELSVKWAGNPNIIVVAKA-FGLKATVQVIDLQVYATPRI 194

Query: 185 IFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIVGGDISSIPGVSDAIEGTIRDAIEGSITW 244
             KPLV  FPCF  +  SL  K  +DF LK++G D+ +IPG+   ++  I+D +     W
Sbjct: 195 TLKPLVPSFPCFANIFVSLMDKPQVDFGLKLLGADVMAIPGLYRFVQEIIKDQVANMYLW 254

Query: 245 PVRNIVPIIAGDYSDLEAKPVGTLEVKLVQAKELTNKDIIGKSDPYAMVFVRPLKERTRT 304
           P    V I+  D S    KPVG L VK+++A +L  KD++G SDPY  + +   K   + 
Sbjct: 255 PKTLNVQIM--DPSKAMKKPVGLLSVKVIKAIKLKKKDLLGGSDPYVKLTLSGDKVPGKK 314

Query: 305 SKTINNQLNPIWNEHFDFVVEDPSTQHLTIRIFDDEGVQASELIGCAQVALKDLEPGKVK 364
           +   ++ LNP WNE FD VV++P +Q L + ++D E V   + IG   + LKDL P + K
Sbjct: 315 TVVKHSNLNPEWNEEFDLVVKEPESQELQLIVYDWEQVGKHDKIGMNVIQLKDLTPEEPK 374

Query: 365 DIWLKLVKDLEIQR--DNKNRGQVHLELLYYPYGTDQSLYMNPFNPDYALTSVEKALKTV 424
            + L+L+K +E +     K+RGQ+ +E+ Y P+  D  +  N  +P+    +VEKA +  
Sbjct: 375 LMTLELLKSMEPKEPVSEKSRGQLVVEVEYKPF-KDDDIPENIDDPN----AVEKAPEGT 434

Query: 425 KSVNTVTNGSEDADSVKQSSPKKRDVIVRGVLSVTVIAAEDLPAVDFMGK--ADPYVVLI 484
            S                           G+L V V  AEDL      GK   +P V L+
Sbjct: 435 PSTG-------------------------GLLVVIVHEAEDLE-----GKYHTNPSVRLL 494

Query: 485 MKKSDTKVKTRVVHDTLNPVWNQTFDF-LVEDALHDMLMVEVWDHDT---FGKDKLGRVI 544
            +  +   KT+ V     P W++ F F L E  ++D L VEV    +     K+ LG V+
Sbjct: 495 FRGEER--KTKRVKKNREPRWDEDFQFPLDEPPINDKLHVEVISSSSRLIHPKETLGYVV 533

Query: 545 MTLTRAILEGEVQDSYPLEGAKSGRIFLHLKW 567
           + L   +    + D Y L  +K+GRI + L+W
Sbjct: 555 INLGDVVSNRRINDKYHLIDSKNGRIQIELQW 533

BLAST of MC11g0443 vs. NCBI nr
Match: XP_022137630.1 (synaptotagmin-4-like [Momordica charantia])

HSP 1 Score: 1138 bits (2944), Expect = 0.0
Identity = 574/575 (99.83%), Postives = 575/575 (100.00%), Query Frame = 0

Query: 1   MSLFFGIFIGVVVGVLLVIAFARAGNVRAKHRSDLATTVAAFARMTAQDSRKILPKEFYP 60
           MSLFFGIFIGVVVGVLLVIAFARAGNVRAKHRSDLATTVAAFARMTAQDSRKILPKEFYP
Sbjct: 1   MSLFFGIFIGVVVGVLLVIAFARAGNVRAKHRSDLATTVAAFARMTAQDSRKILPKEFYP 60

Query: 61  SWVVFTKQQKLTWLNHQLDRIWPFVDAAASELIRSSVEPILEQFRPVVLSSLKFSKLTLG 120
           SWVVFTKQQKLTWLNHQLD+IWPFVDAAASELIRSSVEPILEQFRPVVLSSLKFSKLTLG
Sbjct: 61  SWVVFTKQQKLTWLNHQLDKIWPFVDAAASELIRSSVEPILEQFRPVVLSSLKFSKLTLG 120

Query: 121 TIAPHFTGIAVLEDEAETDGITLELEMQWDGNPNIVLDIKTKVGVSLPVQVKDIAFTGLF 180
           TIAPHFTGIAVLEDEAETDGITLELEMQWDGNPNIVLDIKTKVGVSLPVQVKDIAFTGLF
Sbjct: 121 TIAPHFTGIAVLEDEAETDGITLELEMQWDGNPNIVLDIKTKVGVSLPVQVKDIAFTGLF 180

Query: 181 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIVGGDISSIPGVSDAIEGTIRDAIEGSI 240
           RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIVGGDISSIPGVSDAIEGTIRDAIEGSI
Sbjct: 181 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIVGGDISSIPGVSDAIEGTIRDAIEGSI 240

Query: 241 TWPVRNIVPIIAGDYSDLEAKPVGTLEVKLVQAKELTNKDIIGKSDPYAMVFVRPLKERT 300
           TWPVRNIVPIIAGDYSDLEAKPVGTLEVKLVQAKELTNKDIIGKSDPYAMVFVRPLKERT
Sbjct: 241 TWPVRNIVPIIAGDYSDLEAKPVGTLEVKLVQAKELTNKDIIGKSDPYAMVFVRPLKERT 300

Query: 301 RTSKTINNQLNPIWNEHFDFVVEDPSTQHLTIRIFDDEGVQASELIGCAQVALKDLEPGK 360
           RTSKTINNQLNPIWNEHFDFVVEDPSTQHLTIRIFDDEGVQASELIGCAQVALKDLEPGK
Sbjct: 301 RTSKTINNQLNPIWNEHFDFVVEDPSTQHLTIRIFDDEGVQASELIGCAQVALKDLEPGK 360

Query: 361 VKDIWLKLVKDLEIQRDNKNRGQVHLELLYYPYGTDQSLYMNPFNPDYALTSVEKALKTV 420
           VKDIWLKLVKDLEIQRDNKNRGQVHLELLYYPYGTDQSLYMNPFNPDYALTSVEKALKTV
Sbjct: 361 VKDIWLKLVKDLEIQRDNKNRGQVHLELLYYPYGTDQSLYMNPFNPDYALTSVEKALKTV 420

Query: 421 KSVNTVTNGSEDADSVKQSSPKKRDVIVRGVLSVTVIAAEDLPAVDFMGKADPYVVLIMK 480
           KSVNTVTNGSEDADSVKQSSPKKRDVIVRGVLSVTVIAAEDLPAVDFMGKADPYVVLIMK
Sbjct: 421 KSVNTVTNGSEDADSVKQSSPKKRDVIVRGVLSVTVIAAEDLPAVDFMGKADPYVVLIMK 480

Query: 481 KSDTKVKTRVVHDTLNPVWNQTFDFLVEDALHDMLMVEVWDHDTFGKDKLGRVIMTLTRA 540
           KSDTKVKTRVVHDTLNPVWNQTFDFLVEDALHDMLMVEVWDHDTFGKDKLGRVIMTLTRA
Sbjct: 481 KSDTKVKTRVVHDTLNPVWNQTFDFLVEDALHDMLMVEVWDHDTFGKDKLGRVIMTLTRA 540

Query: 541 ILEGEVQDSYPLEGAKSGRIFLHLKWAAQPIFRDT 575
           ILEGEVQDSYPLEGAKSGRIFLHLKWAAQPIFRDT
Sbjct: 541 ILEGEVQDSYPLEGAKSGRIFLHLKWAAQPIFRDT 575

BLAST of MC11g0443 vs. NCBI nr
Match: XP_038897304.1 (synaptotagmin-4-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1050 bits (2715), Expect = 0.0
Identity = 523/575 (90.96%), Postives = 552/575 (96.00%), Query Frame = 0

Query: 1   MSLFFGIFIGVVVGVLLVIAFARAGNVRAKHRSDLATTVAAFARMTAQDSRKILPKEFYP 60
           MS F GIF+GVVVGVLL+IA ARAGNVRAKHRSDLATT+AAFARMTAQDSRKILPKEFYP
Sbjct: 1   MSFFSGIFLGVVVGVLLIIALARAGNVRAKHRSDLATTIAAFARMTAQDSRKILPKEFYP 60

Query: 61  SWVVFTKQQKLTWLNHQLDRIWPFVDAAASELIRSSVEPILEQFRPVVLSSLKFSKLTLG 120
           SWVVFT++QKLTWLN QLD+IWP+VDAAASELIRS+VEP+LEQFRP +LSSLKFSKLTLG
Sbjct: 61  SWVVFTQRQKLTWLNLQLDKIWPYVDAAASELIRSNVEPVLEQFRPAILSSLKFSKLTLG 120

Query: 121 TIAPHFTGIAVLEDEAETDGITLELEMQWDGNPNIVLDIKTKVGVSLPVQVKDIAFTGLF 180
           T+AP+FTGIAVLEDE ET GITLELEMQWDGNPNIVLDIKTK+GVSLPVQVKDIAFTGLF
Sbjct: 121 TVAPNFTGIAVLEDEPETGGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVKDIAFTGLF 180

Query: 181 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIVGGDISSIPGVSDAIEGTIRDAIEGSI 240
           RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKI+GGDISSIPGVSDAIE TIRDAIEG+I
Sbjct: 181 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIIGGDISSIPGVSDAIEETIRDAIEGTI 240

Query: 241 TWPVRNIVPIIAGDYSDLEAKPVGTLEVKLVQAKELTNKDIIGKSDPYAMVFVRPLKERT 300
           TWPVR IVPIIAGDYSDLEAKPVGTLEVKLVQAKELTNKDIIGKSDPYA++FVRPLKER 
Sbjct: 241 TWPVRKIVPIIAGDYSDLEAKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLFVRPLKERM 300

Query: 301 RTSKTINNQLNPIWNEHFDFVVEDPSTQHLTIRIFDDEGVQASELIGCAQVALKDLEPGK 360
           +TSKTINNQLNPIWNEHFDFVVED STQHLTIR+FDDEGVQASEL+GCAQVALKDLEPGK
Sbjct: 301 KTSKTINNQLNPIWNEHFDFVVEDASTQHLTIRVFDDEGVQASELLGCAQVALKDLEPGK 360

Query: 361 VKDIWLKLVKDLEIQRDNKNRGQVHLELLYYPYGTDQSLYMNPFNPDYALTSVEKALKTV 420
           VKD+WLKLVKDL+IQRDNK RGQVHLELLYYP+GTDQSLY+NPFNPDYALTSVEKALK  
Sbjct: 361 VKDVWLKLVKDLDIQRDNKYRGQVHLELLYYPFGTDQSLYINPFNPDYALTSVEKALKPA 420

Query: 421 KSVNTVTNGSEDADSVKQSSPKKRDVIVRGVLSVTVIAAEDLPAVDFMGKADPYVVLIMK 480
                  NGSEDADS KQSSPKKRDVIVRGVLSVTVIAAEDLPAVDFMGKADPYVVLIMK
Sbjct: 421 P------NGSEDADSGKQSSPKKRDVIVRGVLSVTVIAAEDLPAVDFMGKADPYVVLIMK 480

Query: 481 KSDTKVKTRVVHDTLNPVWNQTFDFLVEDALHDMLMVEVWDHDTFGKDKLGRVIMTLTRA 540
           KS+TKVKTRVVHDTLNPVWNQTFDFLVEDALHDML+VEVWDHDTFGKDKLGRVIMTLTRA
Sbjct: 481 KSETKVKTRVVHDTLNPVWNQTFDFLVEDALHDMLIVEVWDHDTFGKDKLGRVIMTLTRA 540

Query: 541 ILEGEVQDSYPLEGAKSGRIFLHLKWAAQPIFRDT 575
           ILEGE+QD +PLEGAKSGRI+LHLKWAAQPIFRDT
Sbjct: 541 ILEGEIQDCFPLEGAKSGRIYLHLKWAAQPIFRDT 569

BLAST of MC11g0443 vs. NCBI nr
Match: XP_008466436.1 (PREDICTED: synaptotagmin-4-like [Cucumis melo])

HSP 1 Score: 1043 bits (2698), Expect = 0.0
Identity = 516/575 (89.74%), Postives = 551/575 (95.83%), Query Frame = 0

Query: 1   MSLFFGIFIGVVVGVLLVIAFARAGNVRAKHRSDLATTVAAFARMTAQDSRKILPKEFYP 60
           MS F GIFIGVVVGVLL+IA AR GNVRAKHRSDLATT+AAFARMTAQDSRKILPKEFYP
Sbjct: 1   MSFFSGIFIGVVVGVLLIIALARVGNVRAKHRSDLATTIAAFARMTAQDSRKILPKEFYP 60

Query: 61  SWVVFTKQQKLTWLNHQLDRIWPFVDAAASELIRSSVEPILEQFRPVVLSSLKFSKLTLG 120
           SWVVFT++QKLTWLN QLD+IWP+VDAAASELIRS+VEP+LEQFRP++LSSLKFSKLTLG
Sbjct: 61  SWVVFTQRQKLTWLNLQLDKIWPYVDAAASELIRSNVEPVLEQFRPMILSSLKFSKLTLG 120

Query: 121 TIAPHFTGIAVLEDEAETDGITLELEMQWDGNPNIVLDIKTKVGVSLPVQVKDIAFTGLF 180
           ++AP+ TGIAVLEDE++T+GITLELEMQWDGNPNIVLDIKTK+GVSLPVQVKD+AFTGLF
Sbjct: 121 SVAPNLTGIAVLEDESDTNGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVKDVAFTGLF 180

Query: 181 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIVGGDISSIPGVSDAIEGTIRDAIEGSI 240
           RL+FKPLVDEFPCFGAVCYSLRKKKNLDFKLKI+GGDISSIPGVSDAIE TIRDAIEG+I
Sbjct: 181 RLMFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIIGGDISSIPGVSDAIEETIRDAIEGTI 240

Query: 241 TWPVRNIVPIIAGDYSDLEAKPVGTLEVKLVQAKELTNKDIIGKSDPYAMVFVRPLKERT 300
           TWPVR IVPI+AGDYSDLE KPVGTLEVKLVQAKELTNKDIIGKSDPYA++FVRPLKER 
Sbjct: 241 TWPVRQIVPILAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLFVRPLKERM 300

Query: 301 RTSKTINNQLNPIWNEHFDFVVEDPSTQHLTIRIFDDEGVQASELIGCAQVALKDLEPGK 360
           +TSKTINNQLNPIWNEHFDF+VED STQHLTIR+FDDEGVQASELIGCAQVALKDLEPGK
Sbjct: 301 KTSKTINNQLNPIWNEHFDFIVEDASTQHLTIRVFDDEGVQASELIGCAQVALKDLEPGK 360

Query: 361 VKDIWLKLVKDLEIQRDNKNRGQVHLELLYYPYGTDQSLYMNPFNPDYALTSVEKALKTV 420
           VKD+WLKLVKDLEIQRDNK RGQVHLELLYYP+GTDQSLY+NPFNPDYALTSVEKALK  
Sbjct: 361 VKDVWLKLVKDLEIQRDNKYRGQVHLELLYYPFGTDQSLYINPFNPDYALTSVEKALKMA 420

Query: 421 KSVNTVTNGSEDADSVKQSSPKKRDVIVRGVLSVTVIAAEDLPAVDFMGKADPYVVLIMK 480
                  NGSEDADS KQSSPKKRD IVRGVLSVTVIAAEDLPAVDFMGKADPYVVL MK
Sbjct: 421 P------NGSEDADSGKQSSPKKRDTIVRGVLSVTVIAAEDLPAVDFMGKADPYVVLTMK 480

Query: 481 KSDTKVKTRVVHDTLNPVWNQTFDFLVEDALHDMLMVEVWDHDTFGKDKLGRVIMTLTRA 540
           KS+TKVKTRVVHDT+NPVWNQTFDFLVEDALHDMLM+EVWDHDTFGKDKLGRVIMTLTRA
Sbjct: 481 KSETKVKTRVVHDTVNPVWNQTFDFLVEDALHDMLMIEVWDHDTFGKDKLGRVIMTLTRA 540

Query: 541 ILEGEVQDSYPLEGAKSGRIFLHLKWAAQPIFRDT 575
           ILEGE+QDS+PLEGAKSGRIFLHLKWAAQPIFRDT
Sbjct: 541 ILEGEIQDSFPLEGAKSGRIFLHLKWAAQPIFRDT 569

BLAST of MC11g0443 vs. NCBI nr
Match: XP_004136361.2 (synaptotagmin-4 isoform X1 [Cucumis sativus] >KGN60080.1 hypothetical protein Csa_000728 [Cucumis sativus])

HSP 1 Score: 1041 bits (2693), Expect = 0.0
Identity = 517/575 (89.91%), Postives = 550/575 (95.65%), Query Frame = 0

Query: 1   MSLFFGIFIGVVVGVLLVIAFARAGNVRAKHRSDLATTVAAFARMTAQDSRKILPKEFYP 60
           MS F GIF+GVVVGVLLVIAFARAGN RAKHRSDLATT+AAFARMTAQDSRKILPKEFYP
Sbjct: 1   MSFFSGIFLGVVVGVLLVIAFARAGNARAKHRSDLATTIAAFARMTAQDSRKILPKEFYP 60

Query: 61  SWVVFTKQQKLTWLNHQLDRIWPFVDAAASELIRSSVEPILEQFRPVVLSSLKFSKLTLG 120
           SWVVFT++QKLTWLN QLD+IWP+VDAAASELIRS+VEP+LE+FRPV+LSSLKFSKLTLG
Sbjct: 61  SWVVFTQRQKLTWLNLQLDKIWPYVDAAASELIRSNVEPVLEEFRPVILSSLKFSKLTLG 120

Query: 121 TIAPHFTGIAVLEDEAETDGITLELEMQWDGNPNIVLDIKTKVGVSLPVQVKDIAFTGLF 180
           T+AP FTGI+VLEDE +T GITLELEMQWDGNPNIVLDIKTK+GVSLPVQVKDIAFTGLF
Sbjct: 121 TVAPSFTGISVLEDEPDTGGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVKDIAFTGLF 180

Query: 181 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIVGGDISSIPGVSDAIEGTIRDAIEGSI 240
           RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKI+GGDISSIPGVSDAIE TIRDAIEG+I
Sbjct: 181 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIIGGDISSIPGVSDAIEETIRDAIEGTI 240

Query: 241 TWPVRNIVPIIAGDYSDLEAKPVGTLEVKLVQAKELTNKDIIGKSDPYAMVFVRPLKERT 300
           TWPVR IVPI+AGDYSDLE KPVGTLEVKLVQAKELTNKDIIGKSDPYA++FVRPLKER 
Sbjct: 241 TWPVRKIVPILAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLFVRPLKERM 300

Query: 301 RTSKTINNQLNPIWNEHFDFVVEDPSTQHLTIRIFDDEGVQASELIGCAQVALKDLEPGK 360
           +TSKTINNQLNPIWNEHF+F+VED STQHLTIR+FDDEGVQASELIGCAQVALKDLEPGK
Sbjct: 301 KTSKTINNQLNPIWNEHFNFIVEDASTQHLTIRVFDDEGVQASELIGCAQVALKDLEPGK 360

Query: 361 VKDIWLKLVKDLEIQRDNKNRGQVHLELLYYPYGTDQSLYMNPFNPDYALTSVEKALKTV 420
           VKD+WLKLVKDLEIQRDNK RGQVHLELLYYPYGTDQSLY+NPFNPDYALTSVEKALK  
Sbjct: 361 VKDVWLKLVKDLEIQRDNKYRGQVHLELLYYPYGTDQSLYINPFNPDYALTSVEKALKMA 420

Query: 421 KSVNTVTNGSEDADSVKQSSPKKRDVIVRGVLSVTVIAAEDLPAVDFMGKADPYVVLIMK 480
            S      GSEDADS K SSPKKRD IVRGVLSVTVIAAEDLPAVDFMGKADPYVVLIMK
Sbjct: 421 PS------GSEDADSGKPSSPKKRDTIVRGVLSVTVIAAEDLPAVDFMGKADPYVVLIMK 480

Query: 481 KSDTKVKTRVVHDTLNPVWNQTFDFLVEDALHDMLMVEVWDHDTFGKDKLGRVIMTLTRA 540
           KS+TKVKTRVVHDT+NPVWNQTFDFLVEDALHDML+VEVWDHDTFGKDKLGRVIMTLTRA
Sbjct: 481 KSETKVKTRVVHDTVNPVWNQTFDFLVEDALHDMLIVEVWDHDTFGKDKLGRVIMTLTRA 540

Query: 541 ILEGEVQDSYPLEGAKSGRIFLHLKWAAQPIFRDT 575
           ILEGE+QD++PLEGAKSGR+FLHLKWAAQP+FRDT
Sbjct: 541 ILEGEIQDNFPLEGAKSGRVFLHLKWAAQPMFRDT 569

BLAST of MC11g0443 vs. NCBI nr
Match: XP_022937174.1 (synaptotagmin-4 [Cucurbita moschata])

HSP 1 Score: 1028 bits (2659), Expect = 0.0
Identity = 509/575 (88.52%), Postives = 542/575 (94.26%), Query Frame = 0

Query: 1   MSLFFGIFIGVVVGVLLVIAFARAGNVRAKHRSDLATTVAAFARMTAQDSRKILPKEFYP 60
           MS F GIF+GVVVGVLL+IA AR GN+RAKHRSDLA T+AAFARMTAQDSRKILPKEFYP
Sbjct: 1   MSFFSGIFLGVVVGVLLIIALARVGNIRAKHRSDLAKTIAAFARMTAQDSRKILPKEFYP 60

Query: 61  SWVVFTKQQKLTWLNHQLDRIWPFVDAAASELIRSSVEPILEQFRPVVLSSLKFSKLTLG 120
           SWVVFT++QKL WLNHQLD+IWP+VD AASELIRS+VEP+LEQFRP VLSSLKFSKLTLG
Sbjct: 61  SWVVFTQRQKLNWLNHQLDKIWPYVDEAASELIRSNVEPVLEQFRPAVLSSLKFSKLTLG 120

Query: 121 TIAPHFTGIAVLEDEAETDGITLELEMQWDGNPNIVLDIKTKVGVSLPVQVKDIAFTGLF 180
           T+APHFTGIAVLEDE E +GITLELEMQWDGNPNIVLDIKTK+GVSLPVQVKD+AFTGLF
Sbjct: 121 TVAPHFTGIAVLEDELEPNGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVKDVAFTGLF 180

Query: 181 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIVGGDISSIPGVSDAIEGTIRDAIEGSI 240
           RLIFKPLVDEFPCFGA+CYSLRKKKNLDFKLKIVGGDISSIPGVSDAIE TIRDAIEG+I
Sbjct: 181 RLIFKPLVDEFPCFGALCYSLRKKKNLDFKLKIVGGDISSIPGVSDAIEETIRDAIEGTI 240

Query: 241 TWPVRNIVPIIAGDYSDLEAKPVGTLEVKLVQAKELTNKDIIGKSDPYAMVFVRPLKERT 300
           TWPVR IVPIIAGDYSDLE KPVGTLEVKLVQAKELTNKDIIGKSDPYA++FVRPLKER 
Sbjct: 241 TWPVRMIVPIIAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLFVRPLKERM 300

Query: 301 RTSKTINNQLNPIWNEHFDFVVEDPSTQHLTIRIFDDEGVQASELIGCAQVALKDLEPGK 360
           +TSKTINNQLNPIWNEHFDF+VED STQHLTIR+FDDEGVQASELIGCAQVALKDLEPGK
Sbjct: 301 KTSKTINNQLNPIWNEHFDFIVEDASTQHLTIRVFDDEGVQASELIGCAQVALKDLEPGK 360

Query: 361 VKDIWLKLVKDLEIQRDNKNRGQVHLELLYYPYGTDQSLYMNPFNPDYALTSVEKALKTV 420
           VKD+WLKL+KDL+IQRDNKNRGQVHLELLYYP+GTDQ  + NPFNPDYALTSVEK LKT 
Sbjct: 361 VKDVWLKLLKDLDIQRDNKNRGQVHLELLYYPFGTDQGRHKNPFNPDYALTSVEKVLKTA 420

Query: 421 KSVNTVTNGSEDADSVKQSSPKKRDVIVRGVLSVTVIAAEDLPAVDFMGKADPYVVLIMK 480
            +      GSEDA S K SSPKKRD IVRGVLSVTVIAAEDLPAVD+MGKADPYVVLIMK
Sbjct: 421 PT------GSEDAGSRKPSSPKKRDTIVRGVLSVTVIAAEDLPAVDYMGKADPYVVLIMK 480

Query: 481 KSDTKVKTRVVHDTLNPVWNQTFDFLVEDALHDMLMVEVWDHDTFGKDKLGRVIMTLTRA 540
           KS+TKVKTRVVHDTLNPVWNQTFDFLVEDALHDMLMVEVWDHDTFGKDKLGRVIMTLTRA
Sbjct: 481 KSETKVKTRVVHDTLNPVWNQTFDFLVEDALHDMLMVEVWDHDTFGKDKLGRVIMTLTRA 540

Query: 541 ILEGEVQDSYPLEGAKSGRIFLHLKWAAQPIFRDT 575
           ILEGE+QD YPLEGAKSGRI+L+LKWAAQP+FRD 
Sbjct: 541 ILEGEIQDCYPLEGAKSGRIYLNLKWAAQPLFRDN 569

BLAST of MC11g0443 vs. ExPASy TrEMBL
Match: A0A6J1C768 (synaptotagmin-4-like OS=Momordica charantia OX=3673 GN=LOC111009025 PE=3 SV=1)

HSP 1 Score: 1138 bits (2944), Expect = 0.0
Identity = 574/575 (99.83%), Postives = 575/575 (100.00%), Query Frame = 0

Query: 1   MSLFFGIFIGVVVGVLLVIAFARAGNVRAKHRSDLATTVAAFARMTAQDSRKILPKEFYP 60
           MSLFFGIFIGVVVGVLLVIAFARAGNVRAKHRSDLATTVAAFARMTAQDSRKILPKEFYP
Sbjct: 1   MSLFFGIFIGVVVGVLLVIAFARAGNVRAKHRSDLATTVAAFARMTAQDSRKILPKEFYP 60

Query: 61  SWVVFTKQQKLTWLNHQLDRIWPFVDAAASELIRSSVEPILEQFRPVVLSSLKFSKLTLG 120
           SWVVFTKQQKLTWLNHQLD+IWPFVDAAASELIRSSVEPILEQFRPVVLSSLKFSKLTLG
Sbjct: 61  SWVVFTKQQKLTWLNHQLDKIWPFVDAAASELIRSSVEPILEQFRPVVLSSLKFSKLTLG 120

Query: 121 TIAPHFTGIAVLEDEAETDGITLELEMQWDGNPNIVLDIKTKVGVSLPVQVKDIAFTGLF 180
           TIAPHFTGIAVLEDEAETDGITLELEMQWDGNPNIVLDIKTKVGVSLPVQVKDIAFTGLF
Sbjct: 121 TIAPHFTGIAVLEDEAETDGITLELEMQWDGNPNIVLDIKTKVGVSLPVQVKDIAFTGLF 180

Query: 181 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIVGGDISSIPGVSDAIEGTIRDAIEGSI 240
           RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIVGGDISSIPGVSDAIEGTIRDAIEGSI
Sbjct: 181 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIVGGDISSIPGVSDAIEGTIRDAIEGSI 240

Query: 241 TWPVRNIVPIIAGDYSDLEAKPVGTLEVKLVQAKELTNKDIIGKSDPYAMVFVRPLKERT 300
           TWPVRNIVPIIAGDYSDLEAKPVGTLEVKLVQAKELTNKDIIGKSDPYAMVFVRPLKERT
Sbjct: 241 TWPVRNIVPIIAGDYSDLEAKPVGTLEVKLVQAKELTNKDIIGKSDPYAMVFVRPLKERT 300

Query: 301 RTSKTINNQLNPIWNEHFDFVVEDPSTQHLTIRIFDDEGVQASELIGCAQVALKDLEPGK 360
           RTSKTINNQLNPIWNEHFDFVVEDPSTQHLTIRIFDDEGVQASELIGCAQVALKDLEPGK
Sbjct: 301 RTSKTINNQLNPIWNEHFDFVVEDPSTQHLTIRIFDDEGVQASELIGCAQVALKDLEPGK 360

Query: 361 VKDIWLKLVKDLEIQRDNKNRGQVHLELLYYPYGTDQSLYMNPFNPDYALTSVEKALKTV 420
           VKDIWLKLVKDLEIQRDNKNRGQVHLELLYYPYGTDQSLYMNPFNPDYALTSVEKALKTV
Sbjct: 361 VKDIWLKLVKDLEIQRDNKNRGQVHLELLYYPYGTDQSLYMNPFNPDYALTSVEKALKTV 420

Query: 421 KSVNTVTNGSEDADSVKQSSPKKRDVIVRGVLSVTVIAAEDLPAVDFMGKADPYVVLIMK 480
           KSVNTVTNGSEDADSVKQSSPKKRDVIVRGVLSVTVIAAEDLPAVDFMGKADPYVVLIMK
Sbjct: 421 KSVNTVTNGSEDADSVKQSSPKKRDVIVRGVLSVTVIAAEDLPAVDFMGKADPYVVLIMK 480

Query: 481 KSDTKVKTRVVHDTLNPVWNQTFDFLVEDALHDMLMVEVWDHDTFGKDKLGRVIMTLTRA 540
           KSDTKVKTRVVHDTLNPVWNQTFDFLVEDALHDMLMVEVWDHDTFGKDKLGRVIMTLTRA
Sbjct: 481 KSDTKVKTRVVHDTLNPVWNQTFDFLVEDALHDMLMVEVWDHDTFGKDKLGRVIMTLTRA 540

Query: 541 ILEGEVQDSYPLEGAKSGRIFLHLKWAAQPIFRDT 575
           ILEGEVQDSYPLEGAKSGRIFLHLKWAAQPIFRDT
Sbjct: 541 ILEGEVQDSYPLEGAKSGRIFLHLKWAAQPIFRDT 575

BLAST of MC11g0443 vs. ExPASy TrEMBL
Match: A0A1S3CR93 (synaptotagmin-4-like OS=Cucumis melo OX=3656 GN=LOC103503844 PE=3 SV=1)

HSP 1 Score: 1043 bits (2698), Expect = 0.0
Identity = 516/575 (89.74%), Postives = 551/575 (95.83%), Query Frame = 0

Query: 1   MSLFFGIFIGVVVGVLLVIAFARAGNVRAKHRSDLATTVAAFARMTAQDSRKILPKEFYP 60
           MS F GIFIGVVVGVLL+IA AR GNVRAKHRSDLATT+AAFARMTAQDSRKILPKEFYP
Sbjct: 1   MSFFSGIFIGVVVGVLLIIALARVGNVRAKHRSDLATTIAAFARMTAQDSRKILPKEFYP 60

Query: 61  SWVVFTKQQKLTWLNHQLDRIWPFVDAAASELIRSSVEPILEQFRPVVLSSLKFSKLTLG 120
           SWVVFT++QKLTWLN QLD+IWP+VDAAASELIRS+VEP+LEQFRP++LSSLKFSKLTLG
Sbjct: 61  SWVVFTQRQKLTWLNLQLDKIWPYVDAAASELIRSNVEPVLEQFRPMILSSLKFSKLTLG 120

Query: 121 TIAPHFTGIAVLEDEAETDGITLELEMQWDGNPNIVLDIKTKVGVSLPVQVKDIAFTGLF 180
           ++AP+ TGIAVLEDE++T+GITLELEMQWDGNPNIVLDIKTK+GVSLPVQVKD+AFTGLF
Sbjct: 121 SVAPNLTGIAVLEDESDTNGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVKDVAFTGLF 180

Query: 181 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIVGGDISSIPGVSDAIEGTIRDAIEGSI 240
           RL+FKPLVDEFPCFGAVCYSLRKKKNLDFKLKI+GGDISSIPGVSDAIE TIRDAIEG+I
Sbjct: 181 RLMFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIIGGDISSIPGVSDAIEETIRDAIEGTI 240

Query: 241 TWPVRNIVPIIAGDYSDLEAKPVGTLEVKLVQAKELTNKDIIGKSDPYAMVFVRPLKERT 300
           TWPVR IVPI+AGDYSDLE KPVGTLEVKLVQAKELTNKDIIGKSDPYA++FVRPLKER 
Sbjct: 241 TWPVRQIVPILAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLFVRPLKERM 300

Query: 301 RTSKTINNQLNPIWNEHFDFVVEDPSTQHLTIRIFDDEGVQASELIGCAQVALKDLEPGK 360
           +TSKTINNQLNPIWNEHFDF+VED STQHLTIR+FDDEGVQASELIGCAQVALKDLEPGK
Sbjct: 301 KTSKTINNQLNPIWNEHFDFIVEDASTQHLTIRVFDDEGVQASELIGCAQVALKDLEPGK 360

Query: 361 VKDIWLKLVKDLEIQRDNKNRGQVHLELLYYPYGTDQSLYMNPFNPDYALTSVEKALKTV 420
           VKD+WLKLVKDLEIQRDNK RGQVHLELLYYP+GTDQSLY+NPFNPDYALTSVEKALK  
Sbjct: 361 VKDVWLKLVKDLEIQRDNKYRGQVHLELLYYPFGTDQSLYINPFNPDYALTSVEKALKMA 420

Query: 421 KSVNTVTNGSEDADSVKQSSPKKRDVIVRGVLSVTVIAAEDLPAVDFMGKADPYVVLIMK 480
                  NGSEDADS KQSSPKKRD IVRGVLSVTVIAAEDLPAVDFMGKADPYVVL MK
Sbjct: 421 P------NGSEDADSGKQSSPKKRDTIVRGVLSVTVIAAEDLPAVDFMGKADPYVVLTMK 480

Query: 481 KSDTKVKTRVVHDTLNPVWNQTFDFLVEDALHDMLMVEVWDHDTFGKDKLGRVIMTLTRA 540
           KS+TKVKTRVVHDT+NPVWNQTFDFLVEDALHDMLM+EVWDHDTFGKDKLGRVIMTLTRA
Sbjct: 481 KSETKVKTRVVHDTVNPVWNQTFDFLVEDALHDMLMIEVWDHDTFGKDKLGRVIMTLTRA 540

Query: 541 ILEGEVQDSYPLEGAKSGRIFLHLKWAAQPIFRDT 575
           ILEGE+QDS+PLEGAKSGRIFLHLKWAAQPIFRDT
Sbjct: 541 ILEGEIQDSFPLEGAKSGRIFLHLKWAAQPIFRDT 569

BLAST of MC11g0443 vs. ExPASy TrEMBL
Match: A0A0A0LDN3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G876030 PE=3 SV=1)

HSP 1 Score: 1041 bits (2693), Expect = 0.0
Identity = 517/575 (89.91%), Postives = 550/575 (95.65%), Query Frame = 0

Query: 1   MSLFFGIFIGVVVGVLLVIAFARAGNVRAKHRSDLATTVAAFARMTAQDSRKILPKEFYP 60
           MS F GIF+GVVVGVLLVIAFARAGN RAKHRSDLATT+AAFARMTAQDSRKILPKEFYP
Sbjct: 1   MSFFSGIFLGVVVGVLLVIAFARAGNARAKHRSDLATTIAAFARMTAQDSRKILPKEFYP 60

Query: 61  SWVVFTKQQKLTWLNHQLDRIWPFVDAAASELIRSSVEPILEQFRPVVLSSLKFSKLTLG 120
           SWVVFT++QKLTWLN QLD+IWP+VDAAASELIRS+VEP+LE+FRPV+LSSLKFSKLTLG
Sbjct: 61  SWVVFTQRQKLTWLNLQLDKIWPYVDAAASELIRSNVEPVLEEFRPVILSSLKFSKLTLG 120

Query: 121 TIAPHFTGIAVLEDEAETDGITLELEMQWDGNPNIVLDIKTKVGVSLPVQVKDIAFTGLF 180
           T+AP FTGI+VLEDE +T GITLELEMQWDGNPNIVLDIKTK+GVSLPVQVKDIAFTGLF
Sbjct: 121 TVAPSFTGISVLEDEPDTGGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVKDIAFTGLF 180

Query: 181 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIVGGDISSIPGVSDAIEGTIRDAIEGSI 240
           RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKI+GGDISSIPGVSDAIE TIRDAIEG+I
Sbjct: 181 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIIGGDISSIPGVSDAIEETIRDAIEGTI 240

Query: 241 TWPVRNIVPIIAGDYSDLEAKPVGTLEVKLVQAKELTNKDIIGKSDPYAMVFVRPLKERT 300
           TWPVR IVPI+AGDYSDLE KPVGTLEVKLVQAKELTNKDIIGKSDPYA++FVRPLKER 
Sbjct: 241 TWPVRKIVPILAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLFVRPLKERM 300

Query: 301 RTSKTINNQLNPIWNEHFDFVVEDPSTQHLTIRIFDDEGVQASELIGCAQVALKDLEPGK 360
           +TSKTINNQLNPIWNEHF+F+VED STQHLTIR+FDDEGVQASELIGCAQVALKDLEPGK
Sbjct: 301 KTSKTINNQLNPIWNEHFNFIVEDASTQHLTIRVFDDEGVQASELIGCAQVALKDLEPGK 360

Query: 361 VKDIWLKLVKDLEIQRDNKNRGQVHLELLYYPYGTDQSLYMNPFNPDYALTSVEKALKTV 420
           VKD+WLKLVKDLEIQRDNK RGQVHLELLYYPYGTDQSLY+NPFNPDYALTSVEKALK  
Sbjct: 361 VKDVWLKLVKDLEIQRDNKYRGQVHLELLYYPYGTDQSLYINPFNPDYALTSVEKALKMA 420

Query: 421 KSVNTVTNGSEDADSVKQSSPKKRDVIVRGVLSVTVIAAEDLPAVDFMGKADPYVVLIMK 480
            S      GSEDADS K SSPKKRD IVRGVLSVTVIAAEDLPAVDFMGKADPYVVLIMK
Sbjct: 421 PS------GSEDADSGKPSSPKKRDTIVRGVLSVTVIAAEDLPAVDFMGKADPYVVLIMK 480

Query: 481 KSDTKVKTRVVHDTLNPVWNQTFDFLVEDALHDMLMVEVWDHDTFGKDKLGRVIMTLTRA 540
           KS+TKVKTRVVHDT+NPVWNQTFDFLVEDALHDML+VEVWDHDTFGKDKLGRVIMTLTRA
Sbjct: 481 KSETKVKTRVVHDTVNPVWNQTFDFLVEDALHDMLIVEVWDHDTFGKDKLGRVIMTLTRA 540

Query: 541 ILEGEVQDSYPLEGAKSGRIFLHLKWAAQPIFRDT 575
           ILEGE+QD++PLEGAKSGR+FLHLKWAAQP+FRDT
Sbjct: 541 ILEGEIQDNFPLEGAKSGRVFLHLKWAAQPMFRDT 569

BLAST of MC11g0443 vs. ExPASy TrEMBL
Match: A0A6J1FAF6 (synaptotagmin-4 OS=Cucurbita moschata OX=3662 GN=LOC111443550 PE=3 SV=1)

HSP 1 Score: 1028 bits (2659), Expect = 0.0
Identity = 509/575 (88.52%), Postives = 542/575 (94.26%), Query Frame = 0

Query: 1   MSLFFGIFIGVVVGVLLVIAFARAGNVRAKHRSDLATTVAAFARMTAQDSRKILPKEFYP 60
           MS F GIF+GVVVGVLL+IA AR GN+RAKHRSDLA T+AAFARMTAQDSRKILPKEFYP
Sbjct: 1   MSFFSGIFLGVVVGVLLIIALARVGNIRAKHRSDLAKTIAAFARMTAQDSRKILPKEFYP 60

Query: 61  SWVVFTKQQKLTWLNHQLDRIWPFVDAAASELIRSSVEPILEQFRPVVLSSLKFSKLTLG 120
           SWVVFT++QKL WLNHQLD+IWP+VD AASELIRS+VEP+LEQFRP VLSSLKFSKLTLG
Sbjct: 61  SWVVFTQRQKLNWLNHQLDKIWPYVDEAASELIRSNVEPVLEQFRPAVLSSLKFSKLTLG 120

Query: 121 TIAPHFTGIAVLEDEAETDGITLELEMQWDGNPNIVLDIKTKVGVSLPVQVKDIAFTGLF 180
           T+APHFTGIAVLEDE E +GITLELEMQWDGNPNIVLDIKTK+GVSLPVQVKD+AFTGLF
Sbjct: 121 TVAPHFTGIAVLEDELEPNGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVKDVAFTGLF 180

Query: 181 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIVGGDISSIPGVSDAIEGTIRDAIEGSI 240
           RLIFKPLVDEFPCFGA+CYSLRKKKNLDFKLKIVGGDISSIPGVSDAIE TIRDAIEG+I
Sbjct: 181 RLIFKPLVDEFPCFGALCYSLRKKKNLDFKLKIVGGDISSIPGVSDAIEETIRDAIEGTI 240

Query: 241 TWPVRNIVPIIAGDYSDLEAKPVGTLEVKLVQAKELTNKDIIGKSDPYAMVFVRPLKERT 300
           TWPVR IVPIIAGDYSDLE KPVGTLEVKLVQAKELTNKDIIGKSDPYA++FVRPLKER 
Sbjct: 241 TWPVRMIVPIIAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLFVRPLKERM 300

Query: 301 RTSKTINNQLNPIWNEHFDFVVEDPSTQHLTIRIFDDEGVQASELIGCAQVALKDLEPGK 360
           +TSKTINNQLNPIWNEHFDF+VED STQHLTIR+FDDEGVQASELIGCAQVALKDLEPGK
Sbjct: 301 KTSKTINNQLNPIWNEHFDFIVEDASTQHLTIRVFDDEGVQASELIGCAQVALKDLEPGK 360

Query: 361 VKDIWLKLVKDLEIQRDNKNRGQVHLELLYYPYGTDQSLYMNPFNPDYALTSVEKALKTV 420
           VKD+WLKL+KDL+IQRDNKNRGQVHLELLYYP+GTDQ  + NPFNPDYALTSVEK LKT 
Sbjct: 361 VKDVWLKLLKDLDIQRDNKNRGQVHLELLYYPFGTDQGRHKNPFNPDYALTSVEKVLKTA 420

Query: 421 KSVNTVTNGSEDADSVKQSSPKKRDVIVRGVLSVTVIAAEDLPAVDFMGKADPYVVLIMK 480
            +      GSEDA S K SSPKKRD IVRGVLSVTVIAAEDLPAVD+MGKADPYVVLIMK
Sbjct: 421 PT------GSEDAGSRKPSSPKKRDTIVRGVLSVTVIAAEDLPAVDYMGKADPYVVLIMK 480

Query: 481 KSDTKVKTRVVHDTLNPVWNQTFDFLVEDALHDMLMVEVWDHDTFGKDKLGRVIMTLTRA 540
           KS+TKVKTRVVHDTLNPVWNQTFDFLVEDALHDMLMVEVWDHDTFGKDKLGRVIMTLTRA
Sbjct: 481 KSETKVKTRVVHDTLNPVWNQTFDFLVEDALHDMLMVEVWDHDTFGKDKLGRVIMTLTRA 540

Query: 541 ILEGEVQDSYPLEGAKSGRIFLHLKWAAQPIFRDT 575
           ILEGE+QD YPLEGAKSGRI+L+LKWAAQP+FRD 
Sbjct: 541 ILEGEIQDCYPLEGAKSGRIYLNLKWAAQPLFRDN 569

BLAST of MC11g0443 vs. ExPASy TrEMBL
Match: A0A6J1ILB0 (synaptotagmin-4 OS=Cucurbita maxima OX=3661 GN=LOC111476623 PE=3 SV=1)

HSP 1 Score: 1023 bits (2644), Expect = 0.0
Identity = 505/575 (87.83%), Postives = 541/575 (94.09%), Query Frame = 0

Query: 1   MSLFFGIFIGVVVGVLLVIAFARAGNVRAKHRSDLATTVAAFARMTAQDSRKILPKEFYP 60
           MS F GIF+GVVVGVLL+IA AR GN+RAKHRSDLA T+AAFARMTAQDSRKILPKEFYP
Sbjct: 1   MSFFSGIFLGVVVGVLLIIALARVGNIRAKHRSDLAKTIAAFARMTAQDSRKILPKEFYP 60

Query: 61  SWVVFTKQQKLTWLNHQLDRIWPFVDAAASELIRSSVEPILEQFRPVVLSSLKFSKLTLG 120
           SWVVFT++QKL WLNHQLD+IWP+VD AASELIRS+VEP+LEQFRP VLSSLKFSKLTLG
Sbjct: 61  SWVVFTQRQKLNWLNHQLDKIWPYVDEAASELIRSNVEPVLEQFRPAVLSSLKFSKLTLG 120

Query: 121 TIAPHFTGIAVLEDEAETDGITLELEMQWDGNPNIVLDIKTKVGVSLPVQVKDIAFTGLF 180
           T+APHFTGIAVLEDE E +GITLELEMQWDGNPNIVLDIKTK+GVSLPVQVKD+AFTGLF
Sbjct: 121 TVAPHFTGIAVLEDELEPNGITLELEMQWDGNPNIVLDIKTKLGVSLPVQVKDVAFTGLF 180

Query: 181 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIVGGDISSIPGVSDAIEGTIRDAIEGSI 240
           RLIFKPLVDEFPCFGA+CYSLRKKKNLDFKLKIVGGDISSIPGVSDAIE TIRDAIEG+I
Sbjct: 181 RLIFKPLVDEFPCFGALCYSLRKKKNLDFKLKIVGGDISSIPGVSDAIEETIRDAIEGTI 240

Query: 241 TWPVRNIVPIIAGDYSDLEAKPVGTLEVKLVQAKELTNKDIIGKSDPYAMVFVRPLKERT 300
            WPVR IVPIIAGDYSDLE KPVGTLEVKLVQAKELTNKDIIGKSDPYA++FVRPLK+R 
Sbjct: 241 MWPVRMIVPIIAGDYSDLEVKPVGTLEVKLVQAKELTNKDIIGKSDPYAVLFVRPLKDRM 300

Query: 301 RTSKTINNQLNPIWNEHFDFVVEDPSTQHLTIRIFDDEGVQASELIGCAQVALKDLEPGK 360
           +TSKTINNQLNPIWNEHFDFVVED STQHLTIR+FDDEGVQASELIGCAQVALKDLEPGK
Sbjct: 301 KTSKTINNQLNPIWNEHFDFVVEDASTQHLTIRVFDDEGVQASELIGCAQVALKDLEPGK 360

Query: 361 VKDIWLKLVKDLEIQRDNKNRGQVHLELLYYPYGTDQSLYMNPFNPDYALTSVEKALKTV 420
           VKD+WLKL+KDL+IQRDNKNRGQVHLELLYYP+GTDQ  + NPFNPDYALTSVEK LKT 
Sbjct: 361 VKDVWLKLLKDLDIQRDNKNRGQVHLELLYYPFGTDQGRHKNPFNPDYALTSVEKVLKTA 420

Query: 421 KSVNTVTNGSEDADSVKQSSPKKRDVIVRGVLSVTVIAAEDLPAVDFMGKADPYVVLIMK 480
            +      GS+DA S K SSPKKRD IVRGVLSVTVIAAEDLPAVD+MGKADPYVVLIMK
Sbjct: 421 PT------GSDDAGSRKPSSPKKRDTIVRGVLSVTVIAAEDLPAVDYMGKADPYVVLIMK 480

Query: 481 KSDTKVKTRVVHDTLNPVWNQTFDFLVEDALHDMLMVEVWDHDTFGKDKLGRVIMTLTRA 540
           KS+TKVKTRVVHDTLNPVWNQTFDFLVEDALHDMLMVE+WDHDTFGKDKLGRV+MTLTRA
Sbjct: 481 KSETKVKTRVVHDTLNPVWNQTFDFLVEDALHDMLMVELWDHDTFGKDKLGRVVMTLTRA 540

Query: 541 ILEGEVQDSYPLEGAKSGRIFLHLKWAAQPIFRDT 575
           ILEGE+QD YPLEGAKSGRI+L+LKWAAQP+FRD 
Sbjct: 541 ILEGEIQDCYPLEGAKSGRIYLNLKWAAQPLFRDN 569

BLAST of MC11g0443 vs. TAIR 10
Match: AT5G11100.1 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 820.8 bits (2119), Expect = 6.6e-238
Identity = 397/574 (69.16%), Postives = 484/574 (84.32%), Query Frame = 0

Query: 1   MSLFFGIFIGVVVGVLLVIAFARAGNVRAKHRSDLATTVAAFARMTAQDSRKILPKEFYP 60
           M   FG+FIG+ V   LV+AFAR  +VR+  R+DLA T+AAFARMT QDSRK+LP +FYP
Sbjct: 1   MGFLFGLFIGIAVSFGLVVAFARYSSVRSTRRADLAKTIAAFARMTVQDSRKLLPGDFYP 60

Query: 61  SWVVFTKQQKLTWLNHQLDRIWPFVDAAASELIRSSVEPILEQFRPVVLSSLKFSKLTLG 120
           SWVVF+++QKL WLN +L++IWP+V+ AASELI+SSVEP+LEQ+ P +L+SLKFSK TLG
Sbjct: 61  SWVVFSQRQKLNWLNLELEKIWPYVNEAASELIKSSVEPVLEQYTPAMLASLKFSKFTLG 120

Query: 121 TIAPHFTGIAVLEDEAETDGITLELEMQWDGNPNIVLDIKTKVGVSLPVQVKDIAFTGLF 180
           T+AP FTG+++LE E+  +GIT+ELEMQWDGNP IVLD+KT +GVSLP++VK+I FTG+F
Sbjct: 121 TVAPQFTGVSILESESGPNGITMELEMQWDGNPKIVLDVKTLLGVSLPIEVKNIGFTGVF 180

Query: 181 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIVGGDISSIPGVSDAIEGTIRDAIEGSI 240
           RLIFKPLVDEFPCFGA+ YSLR+KK LDF LK++GG+++SIPG+SDAIE TIRDAIE SI
Sbjct: 181 RLIFKPLVDEFPCFGALSYSLREKKGLDFTLKVIGGELTSIPGISDAIEETIRDAIEDSI 240

Query: 241 TWPVRNIVPIIAGDYSDLEAKPVGTLEVKLVQAKELTNKDIIGKSDPYAMVFVRPLKERT 300
           TWPVR I+PI+ GDYSDLE KPVG L+VK+VQAK+L NKD+IGKSDPYA+VF+RPL +RT
Sbjct: 241 TWPVRKIIPILPGDYSDLELKPVGKLDVKVVQAKDLANKDMIGKSDPYAIVFIRPLPDRT 300

Query: 301 RTSKTINNQLNPIWNEHFDFVVEDPSTQHLTIRIFDDEGVQASELIGCAQVALKDLEPGK 360
           + +KTI+N LNPIWNEHF+F+VED STQHLT+R+FDDEGV +S+LIG AQV L +L PGK
Sbjct: 301 KKTKTISNSLNPIWNEHFEFIVEDVSTQHLTVRVFDDEGVGSSQLIGAAQVPLNELVPGK 360

Query: 361 VKDIWLKLVKDLEIQRDNKNRGQVHLELLYYPYGTDQSLYMNPFNPDYALTSVEKALKTV 420
           VKDIWLKLVKDLEIQRD KNRGQV LELLY P G +  L  NPFNPDY+LT +EK LK  
Sbjct: 361 VKDIWLKLVKDLEIQRDTKNRGQVQLELLYCPLGKEGGL-KNPFNPDYSLTILEKVLK-- 420

Query: 421 KSVNTVTNGSEDADSVKQSSPKKRDVIVRGVLSVTVIAAEDLPAVDFMGKADPYVVLIMK 480
                 +  S+  D  K  + KK+DVIVRGVLSVTV+AAEDLPAVDFMGKAD +VV+ +K
Sbjct: 421 ----PESEDSDATDMKKLVTSKKKDVIVRGVLSVTVVAAEDLPAVDFMGKADAFVVITLK 480

Query: 481 KSDTKVKTRVVHDTLNPVWNQTFDFLVEDALHDMLMVEVWDHDTFGKDKLGRVIMTLTRA 540
           KS+TK KTRVV D+LNPVWNQTFDF+VEDALHD+L +EVWDHD FGKDK+GRVIMTLTR 
Sbjct: 481 KSETKSKTRVVPDSLNPVWNQTFDFVVEDALHDLLTLEVWDHDKFGKDKIGRVIMTLTRV 540

Query: 541 ILEGEVQDSYPLEGAKSGRIFLHLKWAAQPIFRD 575
           +LEGE Q+ + L+GAKSG++ +HLKW  +   RD
Sbjct: 541 MLEGEFQEWFELDGAKSGKLCVHLKWTPRLKLRD 567

BLAST of MC11g0443 vs. TAIR 10
Match: AT1G05500.1 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 810.1 bits (2091), Expect = 1.2e-234
Identity = 391/575 (68.00%), Postives = 482/575 (83.83%), Query Frame = 0

Query: 1   MSLFFGIFIGVVVGVLLVIAFARAGNVRAKHRSDLATTVAAFARMTAQDSRKILPKEFYP 60
           M    G+ IG++VG+ ++I F +  N R+K RS+LA TVAAFARMT +DSRK+LP EFYP
Sbjct: 1   MGFIVGVVIGLLVGIAIIIGFVKLENSRSKLRSELANTVAAFARMTVEDSRKLLPPEFYP 60

Query: 61  SWVVFTKQQKLTWLNHQLDRIWPFVDAAASELIRSSVEPILEQFRPVVLSSLKFSKLTLG 120
           SWVVF+++QKLTWLNH L +IWP+VD AASELI++SVEP+LEQ+RP +++SL FSKLTLG
Sbjct: 61  SWVVFSERQKLTWLNHHLTKIWPYVDEAASELIKASVEPVLEQYRPAIVASLTFSKLTLG 120

Query: 121 TIAPHFTGIAVLEDEAETDGITLELEMQWDGNPNIVLDIKTKVGVSLPVQVKDIAFTGLF 180
           T+AP FTG++V+  + + +GITLEL+MQWDGNPNIVL +KT VGVSLP+QVK+I FTG+F
Sbjct: 121 TVAPQFTGVSVI--DGDKNGITLELDMQWDGNPNIVLGVKTLVGVSLPIQVKNIGFTGVF 180

Query: 181 RLIFKPLVDEFPCFGAVCYSLRKKKNLDFKLKIVGGDISSIPGVSDAIEGTIRDAIEGSI 240
           RLIF+PLV++FPCFGAV  SLR+KK LDF LK+VGGDIS+IPG+S+AIE TIRDA+E SI
Sbjct: 181 RLIFRPLVEDFPCFGAVSVSLREKKKLDFTLKVVGGDISAIPGLSEAIEETIRDAVEDSI 240

Query: 241 TWPVRNIVPIIAGDYSDLEAKPVGTLEVKLVQAKELTNKDIIGKSDPYAMVFVRPLKERT 300
           TWPVR ++PII GDYSDLE KPVG LEVKLVQAK LTNKD++GKSDP+A +F+RPL+E+T
Sbjct: 241 TWPVRKVIPIIPGDYSDLELKPVGMLEVKLVQAKNLTNKDLVGKSDPFAKMFIRPLREKT 300

Query: 301 RTSKTINNQLNPIWNEHFDFVVEDPSTQHLTIRIFDDEGVQASELIGCAQVALKDLEPGK 360
           + SKTINN LNPIWNEHF+FVVED STQHL +RI+DDEGVQASELIGCAQ+ L +LEPGK
Sbjct: 301 KRSKTINNDLNPIWNEHFEFVVEDASTQHLVVRIYDDEGVQASELIGCAQIRLCELEPGK 360

Query: 361 VKDIWLKLVKDLEIQRDNKNRGQVHLELLYYPYGTDQSLYMNPFNPDYALTSVEKALKTV 420
           VKD+WLKLVKDLEIQRD KNRG+VHLELLY PYG+   + +NPF    ++TS+E+ LK  
Sbjct: 361 VKDVWLKLVKDLEIQRDTKNRGEVHLELLYIPYGSGNGI-VNPFVTS-SMTSLERVLK-- 420

Query: 421 KSVNTVTNGSEDADSVKQSSPKKRDVIVRGVLSVTVIAAEDLPAVDFMGKADPYVVLIMK 480
                    ++  D    SS K++DVIVRGVLSVTVI+AE++P  D MGKADPYVVL MK
Sbjct: 421 ---------NDTTDEENASSRKRKDVIVRGVLSVTVISAEEIPIQDLMGKADPYVVLSMK 480

Query: 481 KSDTKVKTRVVHDTLNPVWNQTFDFLVEDALHDMLMVEVWDHDTFGKDKLGRVIMTLTRA 540
           KS  K KTRVV+D+LNPVWNQTFDF+VED LHDML++EVWDHDTFGKD +GR I+TLTR 
Sbjct: 481 KSGAKSKTRVVNDSLNPVWNQTFDFVVEDGLHDMLVLEVWDHDTFGKDYIGRCILTLTRV 540

Query: 541 ILEGEVQDSYPLEGAKSGRIFLHLKWAAQPIFRDT 576
           I+E E +D YPL+ +K+G++ LHLKW AQ I+RD+
Sbjct: 541 IMEEEYKDWYPLDESKTGKLQLHLKWMAQSIYRDS 560

BLAST of MC11g0443 vs. TAIR 10
Match: AT3G61050.1 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 288.5 bits (737), Expect = 1.2e-77
Identity = 158/406 (38.92%), Postives = 248/406 (61.08%), Query Frame = 0

Query: 1   MSLFFGIFIGVVVGVLLVIAFARAGNVRAKHRSDLATTVAAFARMTAQDSRKILPKEFYP 60
           M L  GI  G++ GV L+  ++R    R+  R   A  +     ++  D +KI    F P
Sbjct: 1   MGLISGILFGIIFGVALMAGWSRMMTHRSSKRVAKAVDMKLLGSLSRDDLKKICGDNF-P 60

Query: 61  SWVVFTKQQKLTWLNHQLDRIWPFVDAAASELIRSSVEPILEQFRPVVLSSLKFSKLTLG 120
            W+ F   +++ WLN  L ++WP++  AA+ +IR SVEP+LE +RP  ++SLKFSKLTLG
Sbjct: 61  QWISFPAFEQVKWLNKLLSKMWPYIAEAATMVIRDSVEPLLEDYRPPGITSLKFSKLTLG 120

Query: 121 TIAPHFTGIAVLEDEAETDGITLELEMQWDGNPNIVLDIKTKVGVSLPVQVKDIAFTGLF 180
            +AP   GI V     +   +T++++++W G+PNIVL + T +  S+P+Q+KD+    + 
Sbjct: 121 NVAPKIEGIRV--QSFKEGQVTMDVDLRWGGDPNIVLGV-TALVASIPIQLKDLQVFTVA 180

Query: 181 RLIFKPLVDEFPCFGAVCYSL--RKKKNLDFKLKIVGGDISSIPGVSDAIEGTIRDAIEG 240
           R+IF+ L DE PC  AV  +L    K  +D+ LK VGG +++IPG+SD I+ T+   ++ 
Sbjct: 181 RVIFQ-LADEIPCISAVVVALLAEPKPRIDYTLKAVGGSLTAIPGLSDMIDDTVDTIVKD 240

Query: 241 SITWPVRNIVPI--IAGDYSDLEAKPVGTLEVKLVQAKELTNKDIIGKSDPYAMVFVRPL 300
            + WP R +VPI  I  D SDLE KP G L V +V+A  L NK++IGKSDPYA +++RP+
Sbjct: 241 MLQWPHRIVVPIGGIPVDLSDLELKPQGKLIVTVVKATNLKNKELIGKSDPYATIYIRPV 300

Query: 301 -KERTRTSKTINNQLNPIWNEHFDFVVEDPSTQHLTIRIFDDEGVQASELIGCAQVALKD 360
            K +T   K I N LNP+W++ F+ + ED  TQ LT+ +FD + V   E +G  ++ L  
Sbjct: 301 FKYKT---KAIENNLNPVWDQTFELIAEDKETQSLTVEVFDKD-VGQDERLGLVKLPLSS 360

Query: 361 LEPGKVKDIWLKLVKDLEI--QRDNKNRGQVHLELLYYPYGTDQSL 400
           LE G  K++ L L+  L+    +D K+RG + L++ Y+ +  ++ +
Sbjct: 361 LEAGVTKELELNLLSSLDTLKVKDKKDRGSITLKVHYHEFNKEEQM 397

BLAST of MC11g0443 vs. TAIR 10
Match: AT3G61050.2 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 288.5 bits (737), Expect = 1.2e-77
Identity = 158/406 (38.92%), Postives = 248/406 (61.08%), Query Frame = 0

Query: 1   MSLFFGIFIGVVVGVLLVIAFARAGNVRAKHRSDLATTVAAFARMTAQDSRKILPKEFYP 60
           M L  GI  G++ GV L+  ++R    R+  R   A  +     ++  D +KI    F P
Sbjct: 1   MGLISGILFGIIFGVALMAGWSRMMTHRSSKRVAKAVDMKLLGSLSRDDLKKICGDNF-P 60

Query: 61  SWVVFTKQQKLTWLNHQLDRIWPFVDAAASELIRSSVEPILEQFRPVVLSSLKFSKLTLG 120
            W+ F   +++ WLN  L ++WP++  AA+ +IR SVEP+LE +RP  ++SLKFSKLTLG
Sbjct: 61  QWISFPAFEQVKWLNKLLSKMWPYIAEAATMVIRDSVEPLLEDYRPPGITSLKFSKLTLG 120

Query: 121 TIAPHFTGIAVLEDEAETDGITLELEMQWDGNPNIVLDIKTKVGVSLPVQVKDIAFTGLF 180
            +AP   GI V     +   +T++++++W G+PNIVL + T +  S+P+Q+KD+    + 
Sbjct: 121 NVAPKIEGIRV--QSFKEGQVTMDVDLRWGGDPNIVLGV-TALVASIPIQLKDLQVFTVA 180

Query: 181 RLIFKPLVDEFPCFGAVCYSL--RKKKNLDFKLKIVGGDISSIPGVSDAIEGTIRDAIEG 240
           R+IF+ L DE PC  AV  +L    K  +D+ LK VGG +++IPG+SD I+ T+   ++ 
Sbjct: 181 RVIFQ-LADEIPCISAVVVALLAEPKPRIDYTLKAVGGSLTAIPGLSDMIDDTVDTIVKD 240

Query: 241 SITWPVRNIVPI--IAGDYSDLEAKPVGTLEVKLVQAKELTNKDIIGKSDPYAMVFVRPL 300
            + WP R +VPI  I  D SDLE KP G L V +V+A  L NK++IGKSDPYA +++RP+
Sbjct: 241 MLQWPHRIVVPIGGIPVDLSDLELKPQGKLIVTVVKATNLKNKELIGKSDPYATIYIRPV 300

Query: 301 -KERTRTSKTINNQLNPIWNEHFDFVVEDPSTQHLTIRIFDDEGVQASELIGCAQVALKD 360
            K +T   K I N LNP+W++ F+ + ED  TQ LT+ +FD + V   E +G  ++ L  
Sbjct: 301 FKYKT---KAIENNLNPVWDQTFELIAEDKETQSLTVEVFDKD-VGQDERLGLVKLPLSS 360

Query: 361 LEPGKVKDIWLKLVKDLEI--QRDNKNRGQVHLELLYYPYGTDQSL 400
           LE G  K++ L L+  L+    +D K+RG + L++ Y+ +  ++ +
Sbjct: 361 LEAGVTKELELNLLSSLDTLKVKDKKDRGSITLKVHYHEFNKEEQM 397

BLAST of MC11g0443 vs. TAIR 10
Match: AT5G04220.2 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 260.8 bits (665), Expect = 2.6e-69
Identity = 167/570 (29.30%), Postives = 293/570 (51.40%), Query Frame = 0

Query: 6   GIFIGVVVGVLLVIAFARAGNVRAKHRSDLATTVAAFARMTAQDSRKILPKEFYPSWVVF 65
           GI IG+++G  ++I    +       R  + T+++            +LP    P W+  
Sbjct: 16  GIPIGLILGFFVLIYSQPSHQEYPPARPLVETSISVLL--------DLLPD--IPLWMKN 75

Query: 66  TKQQKLTWLNHQLDRIWPFVDAAASELIRSSVEPILEQF-RPVVLSSLKFSKLTLGTIAP 125
              +++ W N  +  +WP++D A   +IRSSV+P+   +     + S++F  L+LGT+ P
Sbjct: 76  PDYERVDWFNKFISYMWPYLDKAVCGIIRSSVQPLFADYIGTFCIESIEFENLSLGTLPP 135

Query: 126 HFTGIAVLEDEAETDGITLELEMQWDGNPNIVLDIKTKVGVSLPVQVKDIAFTGLFRLIF 185
              G+   E   +   +  E  ++W GNPNIVL +K  + + + VQ+ D+ F  + R+  
Sbjct: 136 TVHGVKFYETNEKE--LLFEPSIKWAGNPNIVLVLKV-LSLRIRVQLVDLQFFAIVRVAL 195

Query: 186 KPLVDEFPCFGAVCYSLRKKKNLDFKLKIVGGDISSIPGVSDAIEGTIRDAIEGSITWPV 245
           KPL+  FPCFG V  SL +K ++DF LK++GGD+ SIPG+   ++ TI+  +     WP 
Sbjct: 196 KPLLPTFPCFGMVVVSLMEKPHVDFGLKVLGGDLMSIPGLYRYVQETIKRQVSSMYHWPQ 255

Query: 246 RNIVPIIAGDYSDLEAKPVGTLEVKLVQAKELTNKDIIGKSDPYAMVFVRPLKERTRTSK 305
              +PI+    + ++ KPVG L V +++A+ L  KD++G SDPY  + +   K   + + 
Sbjct: 256 VLEIPILDSSTASVK-KPVGLLHVSILRARNLLKKDLLGTSDPYVKLSLTGEKLPAKKTT 315

Query: 306 TINNQLNPIWNEHFDFVVEDPSTQHLTIRIFDDEGVQASELIGCAQVALKDLEPGKVKDI 365
                LNP WNEHF  +V+DP++Q L + +FD + V   + +G   + L+ + PG+ K+ 
Sbjct: 316 IKKRNLNPEWNEHFKLIVKDPNSQVLQLEVFDWDKVGGHDRLGMQMIPLQKINPGERKEF 375

Query: 366 WLKLVKDLEI---QRDNKNRGQVHLELLYYPYGTDQSLYMNPFNPDYALTSVEKALKTVK 425
            L L+K+  +     D K RG++ ++L Y P+                    E+++K  K
Sbjct: 376 NLDLIKNSNVVMDSGDKKKRGRLEVDLRYVPFR-------------------EESIKRRK 435

Query: 426 SVNTVTNGSEDADSVKQSSPKKRDVIVRGVLSVTVIAAEDLPAVDFMGKADPYVVLIMKK 485
             +     SED D + Q+          G+LSV V +A+D+        ++PY V++ + 
Sbjct: 436 E-SREEKSSEDDDFLSQA----------GLLSVAVQSAKDVEGK--KKHSNPYAVVLFR- 495

Query: 486 SDTKVKTRVVHDTLNPVWNQTFDFLVED-ALHDMLMVEVWDHDT----FGKDKLGRVIMT 545
              K KT+++  T +P WN+ F F +E+  + + + VEV    T      K++LG V + 
Sbjct: 496 -GEKKKTKMLKKTRDPRWNEEFQFTLEEPPVKESIRVEVMSKGTGFHFRSKEELGHVDIN 537

Query: 546 LTRAILEGEVQDSYPLEGAKSGRIFLHLKW 567
           L   +  G +   Y L  +++G I + ++W
Sbjct: 556 LDDVVDNGRINQKYHLINSRNGIIHIEIRW 537

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0JJX59.3e-23769.16Synaptotagmin-4 OS=Arabidopsis thaliana OX=3702 GN=SYT4 PE=2 SV=1[more]
Q8L7061.6e-23368.00Synaptotagmin-5 OS=Arabidopsis thaliana OX=3702 GN=SYT5 PE=2 SV=1[more]
Q9LEX11.7e-7638.92Calcium-dependent lipid-binding protein OS=Arabidopsis thaliana OX=3702 GN=CLB P... [more]
Q7XA063.7e-6829.30Synaptotagmin-3 OS=Arabidopsis thaliana OX=3702 GN=SYT3 PE=2 SV=1[more]
B6ETT46.6e-6531.64Synaptotagmin-2 OS=Arabidopsis thaliana OX=3702 GN=SYT2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022137630.10.099.83synaptotagmin-4-like [Momordica charantia][more]
XP_038897304.10.090.96synaptotagmin-4-like isoform X1 [Benincasa hispida][more]
XP_008466436.10.089.74PREDICTED: synaptotagmin-4-like [Cucumis melo][more]
XP_004136361.20.089.91synaptotagmin-4 isoform X1 [Cucumis sativus] >KGN60080.1 hypothetical protein Cs... [more]
XP_022937174.10.088.52synaptotagmin-4 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1C7680.099.83synaptotagmin-4-like OS=Momordica charantia OX=3673 GN=LOC111009025 PE=3 SV=1[more]
A0A1S3CR930.089.74synaptotagmin-4-like OS=Cucumis melo OX=3656 GN=LOC103503844 PE=3 SV=1[more]
A0A0A0LDN30.089.91Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G876030 PE=3 SV=1[more]
A0A6J1FAF60.088.52synaptotagmin-4 OS=Cucurbita moschata OX=3662 GN=LOC111443550 PE=3 SV=1[more]
A0A6J1ILB00.087.83synaptotagmin-4 OS=Cucurbita maxima OX=3661 GN=LOC111476623 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G11100.16.6e-23869.16Calcium-dependent lipid-binding (CaLB domain) family protein [more]
AT1G05500.11.2e-23468.00Calcium-dependent lipid-binding (CaLB domain) family protein [more]
AT3G61050.11.2e-7738.92Calcium-dependent lipid-binding (CaLB domain) family protein [more]
AT3G61050.21.2e-7738.92Calcium-dependent lipid-binding (CaLB domain) family protein [more]
AT5G04220.22.6e-6929.30Calcium-dependent lipid-binding (CaLB domain) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000008C2 domainPRINTSPR00360C2DOMAINcoord: 515..523
score: 53.62
coord: 467..479
score: 39.27
coord: 493..506
score: 50.66
IPR000008C2 domainSMARTSM00239C2_3ccoord: 451..549
e-value: 1.4E-22
score: 91.1
coord: 265..365
e-value: 4.6E-21
score: 86.0
IPR000008C2 domainPFAMPF00168C2coord: 264..368
e-value: 2.7E-23
score: 82.2
coord: 450..551
e-value: 4.2E-25
score: 88.0
IPR000008C2 domainPROSITEPS50004C2coord: 245..366
score: 20.26465
IPR000008C2 domainPROSITEPS50004C2coord: 432..550
score: 21.258888
IPR039010Synaptotagmin, SMP domainPFAMPF17047SMP_LBDcoord: 70..251
e-value: 8.3E-16
score: 58.0
IPR035892C2 domain superfamilyGENE3D2.60.40.150C2 domaincoord: 256..392
e-value: 1.7E-32
score: 114.3
coord: 431..568
e-value: 1.3E-34
score: 121.2
IPR035892C2 domain superfamilySUPERFAMILY49562C2 domain (Calcium/lipid-binding domain, CaLB)coord: 262..393
IPR035892C2 domain superfamilySUPERFAMILY49562C2 domain (Calcium/lipid-binding domain, CaLB)coord: 449..566
IPR045050Synaptotagmin, plantPANTHERPTHR10774EXTENDED SYNAPTOTAGMIN-RELATEDcoord: 1..574
NoneNo IPR availablePANTHERPTHR10774:SF178SYNAPTOTAGMIN-4coord: 1..574
NoneNo IPR availableCDDcd00030C2coord: 266..368
e-value: 8.04604E-25
score: 97.1374
NoneNo IPR availableCDDcd00030C2coord: 452..552
e-value: 3.14553E-27
score: 103.686
IPR031468Synaptotagmin-like mitochondrial-lipid-binding domainPROSITEPS51847SMPcoord: 67..251
score: 26.938791

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC11g0443.1MC11g0443.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006869 lipid transport
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008289 lipid binding
molecular_function GO:0046872 metal ion binding