MC10g_new0348 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC10g_new0348
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionUnknown protein
LocationMC10: 17095954 .. 17096313 (-)
RNA-Seq ExpressionMC10g_new0348
SyntenyMC10g_new0348
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCATCCTTTTCATATAGTGATCATTCAGCCGGATAGAGGTCATATTATGGGAGCGAAGACGAAGTACCTGGAGAATATGTTACGTTTGTGGATGAAGTTGAGGCGTTTGCTGTTTTGGGAGGGCATTTATTTGGCGATGGAGGCAGGGATCTGTCCAATTCAGCTCGAGACCGATTCAATGCGTGCCTTTAACCTTTTGATTCGAGAGGAGGAAGACCTATCGAGGACAGATTTGATTATTAATCTGGTGAAATCAAATTTCTCGGATATCTTGCATGCATCTTACATTTTCATCAAGAGGGACGAAAACGTAGTCGTGCACTTGCTAGCGCAAAGGGCTTTGCTTCGACAAGAATAA

mRNA sequence

ATGCATCCTTTTCATATAGTGATCATTCAGCCGGATAGAGGTCATATTATGGGAGCGAAGACGAAGTACCTGGAGAATATGTTACGTTTGTGGATGAAGTTGAGGCGTTTGCTGTTTTGGGAGGGCATTTATTTGGCGATGGAGGCAGGGATCTGTCCAATTCAGCTCGAGACCGATTCAATGCGTGCCTTTAACCTTTTGATTCGAGAGGAGGAAGACCTATCGAGGACAGATTTGATTATTAATCTGGTGAAATCAAATTTCTCGGATATCTTGCATGCATCTTACATTTTCATCAAGAGGGACGAAAACGTAGTCGTGCACTTGCTAGCGCAAAGGGCTTTGCTTCGACAAGAATAA

Coding sequence (CDS)

ATGCATCCTTTTCATATAGTGATCATTCAGCCGGATAGAGGTCATATTATGGGAGCGAAGACGAAGTACCTGGAGAATATGTTACGTTTGTGGATGAAGTTGAGGCGTTTGCTGTTTTGGGAGGGCATTTATTTGGCGATGGAGGCAGGGATCTGTCCAATTCAGCTCGAGACCGATTCAATGCGTGCCTTTAACCTTTTGATTCGAGAGGAGGAAGACCTATCGAGGACAGATTTGATTATTAATCTGGTGAAATCAAATTTCTCGGATATCTTGCATGCATCTTACATTTTCATCAAGAGGGACGAAAACGTAGTCGTGCACTTGCTAGCGCAAAGGGCTTTGCTTCGACAAGAATAA

Protein sequence

MHPFHIVIIQPDRGHIMGAKTKYLENMLRLWMKLRRLLFWEGIYLAMEAGICPIQLETDSMRAFNLLIREEEDLSRTDLIINLVKSNFSDILHASYIFIKRDENVVVHLLAQRALLRQE
Homology
BLAST of MC10g_new0348 vs. NCBI nr
Match: XP_022139684.1 (uncharacterized protein LOC111010533 [Momordica charantia])

HSP 1 Score: 93.6 bits (231), Expect = 1.23e-20
Identity = 52/114 (45.61%), Postives = 73/114 (64.04%), Query Frame = 0

Query: 6   IVIIQPDRGHIMGAKTKYLENMLRLWMKLRRLLFWEGIYLAMEAGICPIQLETDSMRAFN 65
           ++II+  RG ++ + TKYLE++  +      L   EG+ +AME GI PI LETDS+R +N
Sbjct: 117 VIIIRDHRGQVLASATKYLEHVASV-DDAEALAAVEGLRVAMETGISPILLETDSLRIYN 176

Query: 66  LLIREEEDLSRTDLIINLVKSNFSDILHASYIFIKRDENVVVHLLAQRALLRQE 119
           L  R++E LS+T  II  VK++ +  L  SY F KR  N + HLLA+RAL  QE
Sbjct: 177 LFARDKEGLSKTGSIIEYVKTHLATRLQVSYSFTKRTGNTIAHLLARRALQSQE 229

BLAST of MC10g_new0348 vs. NCBI nr
Match: XP_022140628.1 (uncharacterized protein LOC111011237 [Momordica charantia])

HSP 1 Score: 92.4 bits (228), Expect = 1.62e-20
Identity = 51/113 (45.13%), Postives = 74/113 (65.49%), Query Frame = 0

Query: 7   VIIQPDRGHIMGAKTKYLENMLRLWMKLRRLLFWEGIYLAMEAGICPIQLETDSMRAFNL 66
           +II+ DRG +M + TKYLEN+  + M    ++  EG+ LA + G+ P+ LETDS R FNL
Sbjct: 61  IIIRNDRGQVMASATKYLENIQSVDMA-EAIVAVEGLQLASKIGVNPVILETDSSRIFNL 120

Query: 67  LIREEEDLSRTDLIINLVKSNFSDILHASYIFIKRDENVVVHLLAQRALLRQE 119
             +  EDLS T  I+   K+ ++  LHAS+ F+KR+ N   H+LA+RALL +E
Sbjct: 121 FSQPSEDLSETGEIVLKAKNFWTQSLHASFNFVKREGNKAAHMLARRALLLRE 172

BLAST of MC10g_new0348 vs. NCBI nr
Match: XP_022150918.1 (uncharacterized protein LOC111018954 [Momordica charantia])

HSP 1 Score: 65.5 bits (158), Expect = 1.74e-09
Identity = 44/113 (38.94%), Postives = 60/113 (53.10%), Query Frame = 0

Query: 7    VIIQPDRGHIMGAKTKYLENMLRLWMKLRRLLFWEGIYLAMEAGICPIQLETDSMRAFNL 66
            +II  DRG +M A TKYLEN+  + M    +   EG+ LA E G+ P             
Sbjct: 1018 IIIHNDRGQVMAAATKYLENIQSVDMA-EAIAAVEGLQLASEIGMHPAL----------- 1077

Query: 67   LIREEEDLSRTDLIINLVKSNFSDILHASYIFIKRDENVVVHLLAQRALLRQE 119
                 EDLS T  I+   K+ ++  LHAS+ F+KR+ N   H+LA+RALL  E
Sbjct: 1078 -----EDLSETGEIVLKAKNFWTQSLHASFNFVKREGNKAAHMLARRALLLHE 1113

BLAST of MC10g_new0348 vs. NCBI nr
Match: XP_022150944.1 (uncharacterized protein LOC111018973 [Momordica charantia])

HSP 1 Score: 55.1 bits (131), Expect = 5.26e-06
Identity = 35/79 (44.30%), Postives = 47/79 (59.49%), Query Frame = 0

Query: 40  WEGIYLAMEAGICPIQLETDSMRAFNLLIREEEDLSRTDLIINLVKSNFSDILHA---SY 99
           +EGI LA+EAG    Q+ETDS+R FNLL  +  D S   ++ +++K   S   HA   S+
Sbjct: 163 YEGILLAVEAGFIRFQIETDSLRIFNLLTTDCVDDSEVGVLCSVIKLFLSS--HAERVSF 222

Query: 100 IFIKRDENVVVHLLAQRAL 115
            F  R+ N   HLLAQ AL
Sbjct: 223 SFTHRNGNAXAHLLAQLAL 239

BLAST of MC10g_new0348 vs. NCBI nr
Match: XP_010684444.1 (PREDICTED: uncharacterized protein LOC104899004 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 52.4 bits (124), Expect = 6.99e-06
Identity = 31/75 (41.33%), Postives = 44/75 (58.67%), Query Frame = 0

Query: 41  EGIYLAMEAGICPIQLETDSMRAFNLLIREEEDLSRTDLIINLVKSNFSDILHASYIFIK 100
           +GI +AMEAG   + +ETDSM  F  L ++ +  S   LI+  + S  S     S+ F+K
Sbjct: 5   QGIRVAMEAGFNSLLVETDSMSLFEALRKKRKPASPFGLILKDIYSLMSQCNQISFSFVK 64

Query: 101 RDENVVVHLLAQRAL 115
           R  N V HLLA+R+L
Sbjct: 65  RAGNEVAHLLAKRSL 79

BLAST of MC10g_new0348 vs. ExPASy TrEMBL
Match: A0A6J1CDQ4 (uncharacterized protein LOC111010533 OS=Momordica charantia OX=3673 GN=LOC111010533 PE=4 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 5.96e-21
Identity = 52/114 (45.61%), Postives = 73/114 (64.04%), Query Frame = 0

Query: 6   IVIIQPDRGHIMGAKTKYLENMLRLWMKLRRLLFWEGIYLAMEAGICPIQLETDSMRAFN 65
           ++II+  RG ++ + TKYLE++  +      L   EG+ +AME GI PI LETDS+R +N
Sbjct: 117 VIIIRDHRGQVLASATKYLEHVASV-DDAEALAAVEGLRVAMETGISPILLETDSLRIYN 176

Query: 66  LLIREEEDLSRTDLIINLVKSNFSDILHASYIFIKRDENVVVHLLAQRALLRQE 119
           L  R++E LS+T  II  VK++ +  L  SY F KR  N + HLLA+RAL  QE
Sbjct: 177 LFARDKEGLSKTGSIIEYVKTHLATRLQVSYSFTKRTGNTIAHLLARRALQSQE 229

BLAST of MC10g_new0348 vs. ExPASy TrEMBL
Match: A0A6J1CIF1 (uncharacterized protein LOC111011237 OS=Momordica charantia OX=3673 GN=LOC111011237 PE=4 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 7.87e-21
Identity = 51/113 (45.13%), Postives = 74/113 (65.49%), Query Frame = 0

Query: 7   VIIQPDRGHIMGAKTKYLENMLRLWMKLRRLLFWEGIYLAMEAGICPIQLETDSMRAFNL 66
           +II+ DRG +M + TKYLEN+  + M    ++  EG+ LA + G+ P+ LETDS R FNL
Sbjct: 61  IIIRNDRGQVMASATKYLENIQSVDMA-EAIVAVEGLQLASKIGVNPVILETDSSRIFNL 120

Query: 67  LIREEEDLSRTDLIINLVKSNFSDILHASYIFIKRDENVVVHLLAQRALLRQE 119
             +  EDLS T  I+   K+ ++  LHAS+ F+KR+ N   H+LA+RALL +E
Sbjct: 121 FSQPSEDLSETGEIVLKAKNFWTQSLHASFNFVKREGNKAAHMLARRALLLRE 172

BLAST of MC10g_new0348 vs. ExPASy TrEMBL
Match: A0A6J1DAR4 (uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018954 PE=4 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 8.43e-10
Identity = 44/113 (38.94%), Postives = 60/113 (53.10%), Query Frame = 0

Query: 7    VIIQPDRGHIMGAKTKYLENMLRLWMKLRRLLFWEGIYLAMEAGICPIQLETDSMRAFNL 66
            +II  DRG +M A TKYLEN+  + M    +   EG+ LA E G+ P             
Sbjct: 1018 IIIHNDRGQVMAAATKYLENIQSVDMA-EAIAAVEGLQLASEIGMHPAL----------- 1077

Query: 67   LIREEEDLSRTDLIINLVKSNFSDILHASYIFIKRDENVVVHLLAQRALLRQE 119
                 EDLS T  I+   K+ ++  LHAS+ F+KR+ N   H+LA+RALL  E
Sbjct: 1078 -----EDLSETGEIVLKAKNFWTQSLHASFNFVKREGNKAAHMLARRALLLHE 1113

BLAST of MC10g_new0348 vs. ExPASy TrEMBL
Match: A0A6J1DBJ7 (uncharacterized protein LOC111018973 OS=Momordica charantia OX=3673 GN=LOC111018973 PE=4 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 2.54e-06
Identity = 35/79 (44.30%), Postives = 47/79 (59.49%), Query Frame = 0

Query: 40  WEGIYLAMEAGICPIQLETDSMRAFNLLIREEEDLSRTDLIINLVKSNFSDILHA---SY 99
           +EGI LA+EAG    Q+ETDS+R FNLL  +  D S   ++ +++K   S   HA   S+
Sbjct: 163 YEGILLAVEAGFIRFQIETDSLRIFNLLTTDCVDDSEVGVLCSVIKLFLSS--HAERVSF 222

Query: 100 IFIKRDENVVVHLLAQRAL 115
            F  R+ N   HLLAQ AL
Sbjct: 223 SFTHRNGNAXAHLLAQLAL 239

BLAST of MC10g_new0348 vs. ExPASy TrEMBL
Match: A0A6J1C467 (uncharacterized protein LOC111007775 OS=Momordica charantia OX=3673 GN=LOC111007775 PE=4 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 7.30e-05
Identity = 27/79 (34.18%), Postives = 47/79 (59.49%), Query Frame = 0

Query: 41  EGIYLAMEAGICPIQLETDSMRAFNLLIREEEDLSRTDLIINLVKSNFSDI-LHASYIFI 100
           EG+ LA+EAG+ P Q+ETDS + FNLL  + ED S   ++ + ++   S + +   + F+
Sbjct: 133 EGVCLAIEAGLIPFQIETDSSQVFNLLRTDCEDESEIGVLASSIRHIVSSLHIGGGFSFV 192

Query: 101 KRDENVVVHLLAQRALLRQ 118
            R+ N   H LA+  ++ +
Sbjct: 193 NREGNSGAHTLARMGMVSE 211

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
XP_022139684.11.23e-2045.61uncharacterized protein LOC111010533 [Momordica charantia][more]
XP_022140628.11.62e-2045.13uncharacterized protein LOC111011237 [Momordica charantia][more]
XP_022150918.11.74e-0938.94uncharacterized protein LOC111018954 [Momordica charantia][more]
XP_022150944.15.26e-0644.30uncharacterized protein LOC111018973 [Momordica charantia][more]
XP_010684444.16.99e-0641.33PREDICTED: uncharacterized protein LOC104899004 [Beta vulgaris subsp. vulgaris][more]
Match NameE-valueIdentityDescription
A0A6J1CDQ45.96e-2145.61uncharacterized protein LOC111010533 OS=Momordica charantia OX=3673 GN=LOC111010... [more]
A0A6J1CIF17.87e-2145.13uncharacterized protein LOC111011237 OS=Momordica charantia OX=3673 GN=LOC111011... [more]
A0A6J1DAR48.43e-1038.94uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018... [more]
A0A6J1DBJ72.54e-0644.30uncharacterized protein LOC111018973 OS=Momordica charantia OX=3673 GN=LOC111018... [more]
A0A6J1C4677.30e-0534.18uncharacterized protein LOC111007775 OS=Momordica charantia OX=3673 GN=LOC111007... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 40..114
e-value: 2.5E-8
score: 33.8
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 7..111
e-value: 3.89322E-9
score: 48.8496

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC10g_new0348.1MC10g_new0348.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity