MC10g_new0157 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC10g_new0157
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionepidermis-specific secreted glycoprotein EP1-like
LocationMC10: 7700184 .. 7701879 (+)
RNA-Seq ExpressionMC10g_new0157
SyntenyMC10g_new0157
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCAAACAGCACTTAAAGGGACCACCTATATCCAAAACTATCAAATACAATGGACAGTAATGGAAAGACCAGGCAGCCAAAAACAGATCAATAAATAAAGATTTTTTTATTCTAATCTGAAATTACACAAATACCCTTGTCAGCTCCTATAAATACACATCTTCGTCTTCGTCCATTCCTCATTATCCAGTTCTTCAAGTTAACAGCAAACATGAGAATTCCGCCATTGTTGACGCCTCTGCTGCTCTCTTGCTTCTTCCTCTCTCTCTCTCAAGCTCTGGTTCCTCCCGACCAGACCTTCAAGTTCGTCAATGAGGGTGACTTCGGCGACTTCGCCGTCGAGTACGGCGCCAATTACAGAGTCCTCAGCATCGCGCAATCGCCGTTTCAGCTCGCGTTCTACAACACTACGCCGAACGCCTACACTCTGGCTCTGCGGATGGCGATCCTCCGCTCCGAATCGGCGATGCGGTGGGTGTGGGAGGCCAATCGCGGCCGTCCGGTGCGCGACAACGCCACTTTCTCCCTCGGCGCCGACGGAAACCTAGTCCTCGCCGATTCCGACGGCTCCGTCGTCTGGCAATCGAACACCGCCAACAAAGGCGTCGTCGGATTCAAATTGCTCCCCAACGGCAACATGGTCCTCCACGACTCCAGCGGCAAATTCCTCTGGCAGAGCTTCGATTCCCCGACGGATACTCTGTTAGTCGGCCAATCGCTGCGCCTCGGCGGCGCAATCAAGCTCGTAAGCCGCGCCTCCGAGAAATTAAACTCCAACGGACCGTACAGCTTCGTAATGGAGAAAAAAACCCTAAATCTCTACTACAAAAGCCCTAATTCCCCGAAGCCGATGCGCTACTTCACATCCTCAAATAGGTTCACAATCCAGAAAGGATCTCTCTCCAAGGTCACTCTCAACGCCGATGTAGATCCAGATCAAGGATTCGCCACAGAAATAAAGCTAGACTACGAATCCGCCGGCTCGACGGAGAGCGGCGGCACGATTGTGGCGCGGCCGAAGTACAACGCCACATTGACGTTCCTCCGGCTGGGAATCGACGGCAATCTCCGCCTCATCACATACAACGACAAGGTCGATTGGGGCCCGTCAGAGGTTTCGTTCACGCTGTTCGACAGAGACTCGAATTGGGAGAGCGAGTGCCAGTGGCCGGAGCGGTGCGGGCAGTTGGGGCTGTGTGAGGAGAACCAATGCGTGGGCTGCCCGACGGCGAACGGCGTGGTGGGGTGGAGCAAGAGCTGCGCGGCGGAGAAGGTGAGATCCTGCGATCCGAAGAGCTTCCATTACTACAAAATCGAAGGTGTGGATCATTTTCTGGCGAAGTATAACAGAGGAGAAGGGCCGATGGGGCAGAGGGAGTGCGAGAAGAAGTGCAGTTTGGACTGCAAATGTTTGGGGTATTTTTATCAAACCAAAGGGTCGCTTTGTTGGGTTGCGAATGAGTTGAAAACTCTCATAAAAGTGGCCAACTCCACTCATTTGGGGTTCATCAAAACGCCCAATAAGTAGAACAAACAGAGAGTTTCTTTTCCTCTCATGGAATATTTGTAATATTGTTGCAACTTGTAAGTGTAAGATGTGTGTGTTATAATAATGCTTTCCCTTTTGTAGTTTTCAAATAATATTGAGGGTTGGTTTAAAATTTAAATACAATTTGGCTCCTAATTTGGTTCATCT

mRNA sequence

ATGAGAATTCCGCCATTGTTGACGCCTCTGCTGCTCTCTTGCTTCTTCCTCTCTCTCTCTCAAGCTCTGGTTCCTCCCGACCAGACCTTCAAGTTCGTCAATGAGGGTGACTTCGGCGACTTCGCCGTCGAGTACGGCGCCAATTACAGAGTCCTCAGCATCGCGCAATCGCCGTTTCAGCTCGCGTTCTACAACACTACGCCGAACGCCTACACTCTGGCTCTGCGGATGGCGATCCTCCGCTCCGAATCGGCGATGCGGTGGGTGTGGGAGGCCAATCGCGGCCGTCCGGTGCGCGACAACGCCACTTTCTCCCTCGGCGCCGACGGAAACCTAGTCCTCGCCGATTCCGACGGCTCCGTCGTCTGGCAATCGAACACCGCCAACAAAGGCGTCGTCGGATTCAAATTGCTCCCCAACGGCAACATGGTCCTCCACGACTCCAGCGGCAAATTCCTCTGGCAGAGCTTCGATTCCCCGACGGATACTCTGTTAGTCGGCCAATCGCTGCGCCTCGGCGGCGCAATCAAGCTCGTAAGCCGCGCCTCCGAGAAATTAAACTCCAACGGACCGTACAGCTTCGTAATGGAGAAAAAAACCCTAAATCTCTACTACAAAAGCCCTAATTCCCCGAAGCCGATGCGCTACTTCACATCCTCAAATAGGTTCACAATCCAGAAAGGATCTCTCTCCAAGGTCACTCTCAACGCCGATGTAGATCCAGATCAAGGATTCGCCACAGAAATAAAGCTAGACTACGAATCCGCCGGCTCGACGGAGAGCGGCGGCACGATTGTGGCGCGGCCGAAGTACAACGCCACATTGACGTTCCTCCGGCTGGGAATCGACGGCAATCTCCGCCTCATCACATACAACGACAAGGTCGATTGGGGCCCGTCAGAGGTTTCGTTCACGCTGTTCGACAGAGACTCGAATTGGGAGAGCGAGTGCCAGTGGCCGGAGCGGTGCGGGCAGTTGGGGCTGTGTGAGGAGAACCAATGCGTGGGCTGCCCGACGGCGAACGGCGTGGTGGGGTGGAGCAAGAGCTGCGCGGCGGAGAAGGTGAGATCCTGCGATCCGAAGAGCTTCCATTACTACAAAATCGAAGGTGTGGATCATTTTCTGGCGAAGTATAACAGAGGAGAAGGGCCGATGGGGCAGAGGGAGTGCGAGAAGAAGTGCAGTTTGGACTGCAAATGTTTGGGGTATTTTTATCAAACCAAAGGGTCGCTTTGTTGGGTTGCGAATGAGTTGAAAACTCTCATAAAAGTGGCCAACTCCACTCATTTGGGGTTCATCAAAACGCCCAATAAGTAG

Coding sequence (CDS)

ATGAGAATTCCGCCATTGTTGACGCCTCTGCTGCTCTCTTGCTTCTTCCTCTCTCTCTCTCAAGCTCTGGTTCCTCCCGACCAGACCTTCAAGTTCGTCAATGAGGGTGACTTCGGCGACTTCGCCGTCGAGTACGGCGCCAATTACAGAGTCCTCAGCATCGCGCAATCGCCGTTTCAGCTCGCGTTCTACAACACTACGCCGAACGCCTACACTCTGGCTCTGCGGATGGCGATCCTCCGCTCCGAATCGGCGATGCGGTGGGTGTGGGAGGCCAATCGCGGCCGTCCGGTGCGCGACAACGCCACTTTCTCCCTCGGCGCCGACGGAAACCTAGTCCTCGCCGATTCCGACGGCTCCGTCGTCTGGCAATCGAACACCGCCAACAAAGGCGTCGTCGGATTCAAATTGCTCCCCAACGGCAACATGGTCCTCCACGACTCCAGCGGCAAATTCCTCTGGCAGAGCTTCGATTCCCCGACGGATACTCTGTTAGTCGGCCAATCGCTGCGCCTCGGCGGCGCAATCAAGCTCGTAAGCCGCGCCTCCGAGAAATTAAACTCCAACGGACCGTACAGCTTCGTAATGGAGAAAAAAACCCTAAATCTCTACTACAAAAGCCCTAATTCCCCGAAGCCGATGCGCTACTTCACATCCTCAAATAGGTTCACAATCCAGAAAGGATCTCTCTCCAAGGTCACTCTCAACGCCGATGTAGATCCAGATCAAGGATTCGCCACAGAAATAAAGCTAGACTACGAATCCGCCGGCTCGACGGAGAGCGGCGGCACGATTGTGGCGCGGCCGAAGTACAACGCCACATTGACGTTCCTCCGGCTGGGAATCGACGGCAATCTCCGCCTCATCACATACAACGACAAGGTCGATTGGGGCCCGTCAGAGGTTTCGTTCACGCTGTTCGACAGAGACTCGAATTGGGAGAGCGAGTGCCAGTGGCCGGAGCGGTGCGGGCAGTTGGGGCTGTGTGAGGAGAACCAATGCGTGGGCTGCCCGACGGCGAACGGCGTGGTGGGGTGGAGCAAGAGCTGCGCGGCGGAGAAGGTGAGATCCTGCGATCCGAAGAGCTTCCATTACTACAAAATCGAAGGTGTGGATCATTTTCTGGCGAAGTATAACAGAGGAGAAGGGCCGATGGGGCAGAGGGAGTGCGAGAAGAAGTGCAGTTTGGACTGCAAATGTTTGGGGTATTTTTATCAAACCAAAGGGTCGCTTTGTTGGGTTGCGAATGAGTTGAAAACTCTCATAAAAGTGGCCAACTCCACTCATTTGGGGTTCATCAAAACGCCCAATAAGTAG

Protein sequence

MRIPPLLTPLLLSCFFLSLSQALVPPDQTFKFVNEGDFGDFAVEYGANYRVLSIAQSPFQLAFYNTTPNAYTLALRMAILRSESAMRWVWEANRGRPVRDNATFSLGADGNLVLADSDGSVVWQSNTANKGVVGFKLLPNGNMVLHDSSGKFLWQSFDSPTDTLLVGQSLRLGGAIKLVSRASEKLNSNGPYSFVMEKKTLNLYYKSPNSPKPMRYFTSSNRFTIQKGSLSKVTLNADVDPDQGFATEIKLDYESAGSTESGGTIVARPKYNATLTFLRLGIDGNLRLITYNDKVDWGPSEVSFTLFDRDSNWESECQWPERCGQLGLCEENQCVGCPTANGVVGWSKSCAAEKVRSCDPKSFHYYKIEGVDHFLAKYNRGEGPMGQRECEKKCSLDCKCLGYFYQTKGSLCWVANELKTLIKVANSTHLGFIKTPNK
Homology
BLAST of MC10g_new0157 vs. ExPASy Swiss-Prot
Match: Q9ZVA4 (EP1-like glycoprotein 3 OS=Arabidopsis thaliana OX=3702 GN=At1g78850 PE=1 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 5.4e-120
Identity = 215/433 (49.65%), Postives = 288/433 (66.51%), Query Frame = 0

Query: 11  LLSCFFLSL----SQALVPPDQTFKFVNEGDFGDFA-VEYGANYRVLSIAQSPFQLAFYN 70
           L  CF LS+    SQA VP D  F+ VNEG + D++ +EY  + R        F+L FYN
Sbjct: 7   LALCFTLSIFLIGSQAKVPVDDQFRVVNEGGYTDYSPIEYNPDVRGFVPFSDNFRLCFYN 66

Query: 71  TTPNAYTLALRMAILRSESAMRWVWEANRGRPVRDNATFSLGADGNLVLADSDGSVVWQS 130
           TTPNAYTLALR+     ES +RWVWEANRG PV++NAT + G DGNLVLA++DG +VWQ+
Sbjct: 67  TTPNAYTLALRIGNRVQESTLRWVWEANRGSPVKENATLTFGEDGNLVLAEADGRLVWQT 126

Query: 131 NTANKGVVGFKLLPNGNMVLHDSSGKFLWQSFDSPTDTLLVGQSLRLGGAIKLVSRASEK 190
           NTANKG VG K+L NGNMV++DSSGKF+WQSFDSPTDTLLVGQSL+L G  KLVSR S  
Sbjct: 127 NTANKGAVGIKILENGNMVIYDSSGKFVWQSFDSPTDTLLVGQSLKLNGRTKLVSRLSPS 186

Query: 191 LNSNGPYSFVMEKKTLNLYYKSPNSPKPMRYFTSSNRFTIQKGSLSKVTLNADVDPDQGF 250
           +N+NGPYS VME K L LYY +  +PKP+ YF     F  +      +T  A  D D  +
Sbjct: 187 VNTNGPYSLVMEAKKLVLYYTTNKTPKPIAYF--EYEFFTKITQFQSMTFQAVEDSDTTW 246

Query: 251 ATEIKLDYESAGSTESGGTIVARPKYNATLTFLRLGIDGNLRLITYNDKVDWGPSEVSFT 310
              + ++   +GS  +  T ++RPK+NATL+F+RL  DGN+R+ +Y+        +V++T
Sbjct: 247 G--LVMEGVDSGSKFNVSTFLSRPKHNATLSFIRLESDGNIRVWSYSTLATSTAWDVTYT 306

Query: 311 LF-DRDSNWESECQWPERCGQLGLCEENQCVGCPTANGVVGWSKSCAAEKVRSCDPKSFH 370
            F + D++   EC+ PE C   GLC++ QC  CP+  G++GW ++C +  + SCDPK+FH
Sbjct: 307 AFTNADTDGNDECRIPEHCLGFGLCKKGQCNACPSDKGLLGWDETCKSPSLASCDPKTFH 366

Query: 371 YYKIEGVDHFLAKYNRGEGPMGQRECEKKCSLDCKCLGYFYQTKGSLCWVANELKTLIKV 430
           Y+KIEG D F+ KYN G     +  C  KC+ DCKCLG+FY  K S CW+  ELKTL + 
Sbjct: 367 YFKIEGADSFMTKYNGGSSTT-ESACGDKCTRDCKCLGFFYNRKSSRCWLGYELKTLTRT 426

Query: 431 ANSTHLGFIKTPN 438
            +S+ + ++K PN
Sbjct: 427 GDSSLVAYVKAPN 434

BLAST of MC10g_new0157 vs. ExPASy Swiss-Prot
Match: Q9ZVA5 (EP1-like glycoprotein 4 OS=Arabidopsis thaliana OX=3702 GN=At1g78860 PE=3 SV=1)

HSP 1 Score: 427.6 bits (1098), Expect = 1.8e-118
Identity = 212/424 (50.00%), Postives = 283/424 (66.75%), Query Frame = 0

Query: 16  FLSLSQALVPPDQTFKFVNEGDFGDFA-VEYGANYRVLSIAQSPFQLAFYNTTPNAYTLA 75
           FL  +QA VP D  F+ VNEG + D++ +EY  + R        F+L FYNTT NAYTLA
Sbjct: 16  FLVGAQAKVPVDDQFRVVNEGGYTDYSPIEYNPDVRGFVPFSDNFRLCFYNTTQNAYTLA 75

Query: 76  LRMAILRSESAMRWVWEANRGRPVRDNATFSLGADGNLVLADSDGSVVWQSNTANKGVVG 135
           LR+     ES +RWVWEANRG PV++NAT + G DGNLVLA++DG VVWQ+NTANKGVVG
Sbjct: 76  LRIGNRAQESTLRWVWEANRGSPVKENATLTFGEDGNLVLAEADGRVVWQTNTANKGVVG 135

Query: 136 FKLLPNGNMVLHDSSGKFLWQSFDSPTDTLLVGQSLRLGGAIKLVSRASEKLNSNGPYSF 195
            K+L NGNMV++DS+GKF+WQSFDSPTDTLLVGQSL+L G  KLVSR S  +N+NGPYS 
Sbjct: 136 IKILENGNMVIYDSNGKFVWQSFDSPTDTLLVGQSLKLNGQNKLVSRLSPSVNANGPYSL 195

Query: 196 VMEKKTLNLYYKSPNSPKPMRYFTSSNRFTIQKGSLSKVTLNADVDPDQGFATEIKLDYE 255
           VME K L LYY +  +PKP+ Y+     F  +   L  +T  A  D D  +   + ++  
Sbjct: 196 VMEAKKLVLYYTTNKTPKPIGYY--EYEFFTKIAQLQSMTFQAVEDADTTWG--LHMEGV 255

Query: 256 SAGSTESGGTIVARPKYNATLTFLRLGIDGNLRLITYNDKVDWGPSEVSFTLFDRD-SNW 315
            +GS  +  T ++RPK+NATL+FLRL  DGN+R+ +Y+        +V++T F  D ++ 
Sbjct: 256 DSGSQFNVSTFLSRPKHNATLSFLRLESDGNIRVWSYSTLATSTAWDVTYTAFTNDNTDG 315

Query: 316 ESECQWPERCGQLGLCEENQCVGCPTANGVVGWSKSCAAEKVRSCDPKSFHYYKIEGVDH 375
             EC+ PE C   GLC++ QC  CP+  G++GW ++C    + SCDPK+FHY+KIEG D 
Sbjct: 316 NDECRIPEHCLGFGLCKKGQCNACPSDIGLLGWDETCKIPSLASCDPKTFHYFKIEGADS 375

Query: 376 FLAKYNRGEGPMGQRECEKKCSLDCKCLGYFYQTKGSLCWVANELKTLIKVANSTHLGFI 435
           F+ KYN G     +  C  KC+ DCKCLG+FY  K S CW+  ELKTL K  +++ + ++
Sbjct: 376 FMTKYN-GGSTTTESACGDKCTRDCKCLGFFYNRKSSRCWLGYELKTLTKTGDTSLVAYV 434

Query: 436 KTPN 438
           K PN
Sbjct: 436 KAPN 434

BLAST of MC10g_new0157 vs. ExPASy Swiss-Prot
Match: Q39688 (Epidermis-specific secreted glycoprotein EP1 OS=Daucus carota OX=4039 GN=EP1 PE=1 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 4.8e-116
Identity = 216/381 (56.69%), Postives = 265/381 (69.55%), Query Frame = 0

Query: 4   PPLLTPLLLSCFFLSLSQALVPPDQTFKFVNEGDFGDFAVEYGANYRVLSIAQSPFQLAF 63
           P  LT LL     +     LVP ++TFKFVNEG+ G +  EY  +YR L    SPFQL F
Sbjct: 6   PLTLTILLFFIQRIDFCHTLVPANETFKFVNEGELGQYISEYFGDYRPLDPFTSPFQLCF 65

Query: 64  YNTTPNAYTLALRMAILRSESAMRWVWEANRGRPVRDNATFSLGADGNLVLADSDGSVVW 123
           YN TP A+TLALRM + R+ES MRWVWEANRG PV +NAT + G DGNLVLA S+G V W
Sbjct: 66  YNQTPTAFTLALRMGLRRTESLMRWVWEANRGNPVDENATLTFGPDGNLVLARSNGQVAW 125

Query: 124 QSNTANKGVVGFKLLPNGNMVLHDSSGKFLWQSFDSPTDTLLVGQSLRLGGAIKLVSRAS 183
           Q++TANKGVVG K+LPNGNMVL+DS GKFLWQSFD+PTDTLLVGQSL++G   KLVSRAS
Sbjct: 126 QTSTANKGVVGLKILPNGNMVLYDSKGKFLWQSFDTPTDTLLVGQSLKMGAVTKLVSRAS 185

Query: 184 EKLNSNGPYSFVMEKKTLNLYYKSPNSPKPMRYFTSSNRFTIQKG-SLSKVTLNADVDPD 243
              N NGPYS VME K L+LYYK   SPKP+RY++ S    + K  SL  VT   + + D
Sbjct: 186 PGENVNGPYSLVMEPKGLHLYYKPTTSPKPIRYYSFSLFTKLNKNESLQNVTFEFENEND 245

Query: 244 QGFATEIKLDYESAGSTESGGTIVARPKYNATLTFLRLGIDGNLRLITYNDKVDWGPSEV 303
           QGFA  + L Y ++ S   G +I+ R KYN TL+FLRL IDGN+++ TYNDKVD+G  EV
Sbjct: 246 QGFAFLLSLKYGTSNSL-GGASILNRIKYNTTLSFLRLEIDGNVKIYTYNDKVDYGAWEV 305

Query: 304 SFTLFDR------------DSNWESECQWPERCGQLGLCEENQCVGCPTANG-VVGWSKS 363
           ++TLF +              +  SECQ P++CG  GLCEE+QCVGCPT++G V+ WSK+
Sbjct: 306 TYTLFLKAPPPLFQVSLAATESESSECQLPKKCGNFGLCEESQCVGCPTSSGPVLAWSKT 365

Query: 364 CAAEKVRSCDPKSFHYYKIEG 371
           C   K+ SC PK FHY K+ G
Sbjct: 366 CEPPKLSSCGPKDFHYNKLGG 385

BLAST of MC10g_new0157 vs. ExPASy Swiss-Prot
Match: Q9ZVA2 (EP1-like glycoprotein 2 OS=Arabidopsis thaliana OX=3702 GN=At1g78830 PE=1 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 3.5e-103
Identity = 203/453 (44.81%), Postives = 273/453 (60.26%), Query Frame = 0

Query: 6   LLTPLLLSCFFLSLSQALVPPDQTFKFVNEGDFGDFAVEYGANYRVL-----SIAQSPFQ 65
           +L  L L+   +S+  A VPP++ F+ VNEG+FG++  EY A+YR +     S   SPFQ
Sbjct: 6   ILVTLALAIATVSVVIAQVPPEKQFRVVNEGEFGEYITEYDASYRFIESSNQSFFTSPFQ 65

Query: 66  LAFYNTTPNAYTLALRMAILRSESAMRWVWEANRGRPVRDNATFSLGADGNLVLADSDGS 125
           L FYNTTP+AY LALR+ + R ES MRW+W+ANR  PV +NAT SLG +GNLVLA++DG 
Sbjct: 66  LLFYNTTPSAYILALRVGLRRDESTMRWIWDANRNNPVGENATLSLGRNGNLVLAEADGR 125

Query: 126 VVWQSNTANKGVVGFKLLPNGNMVLHDSSGKFLWQSFDSPTDTLLVGQSLRLGGAIKLVS 185
           V WQ+NTANKGV GF++LPNGN+VLHD +GKF+WQSFD PTDTLL GQSL++ G  KLVS
Sbjct: 126 VKWQTNTANKGVTGFQILPNGNIVLHDKNGKFVWQSFDHPTDTLLTGQSLKVNGVNKLVS 185

Query: 186 RASEKLNSNGPYSFVMEKKTLNLYYKSPNSPK-----PMRYFTSSNRFTIQK-------G 245
           R S+   S+GPYS V++KK L +Y     +P      P   F  +  F + +        
Sbjct: 186 RTSDSNGSDGPYSMVLDKKGLTMYVNKTGTPLVYGGWPDHDFRGTVTFAVTREFDNLTEP 245

Query: 246 SLSKVTLNADVDPDQGFATEIK-LDYESAGSTESGGTI-VARPKYNATLTFLRLGIDGNL 305
           S  ++ L     P        + L     GS   GGT+ + +  YN T+++LRLG DG+L
Sbjct: 246 SAYELLLEPAPQPATNPGNNRRLLQVRPIGS--GGGTLNLNKINYNGTISYLRLGSDGSL 305

Query: 306 RLITYNDKVDWGPSEVSFTLFDRDSNWESECQWPERCGQLGLCEENQCVGCPTANGVVGW 365
           +  +Y     +   E SF+ F   + +  +C  P  CG  G C+   C  CPT  G++GW
Sbjct: 306 KAYSYFPAATYLKWEESFSFF--STYFVRQCGLPSFCGDYGYCDRGMCNACPTPKGLLGW 365

Query: 366 SKSCAAEKV----RSCDPKSFHYYKIEGVDHFLAKY-NRGEGPMGQRECEKKCSLDCKCL 425
           S  CA  K          K+ +YYKI GV+HF   Y N G+GP    +C+ KC  DCKCL
Sbjct: 366 SDKCAPPKTTQFCSGVKGKTVNYYKIVGVEHFTGPYVNDGQGPTSVNDCKAKCDRDCKCL 425

Query: 426 GYFYQTKGSLCWVANELKTLIKVANSTHLGFIK 435
           GYFY+ K   C +A  L TLIK AN++ + +IK
Sbjct: 426 GYFYKEKDKKCLLAPLLGTLIKDANTSSVAYIK 454

BLAST of MC10g_new0157 vs. ExPASy Swiss-Prot
Match: Q9ZVA1 (EP1-like glycoprotein 1 OS=Arabidopsis thaliana OX=3702 GN=At1g78820 PE=2 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 1.9e-96
Identity = 194/455 (42.64%), Postives = 267/455 (58.68%), Query Frame = 0

Query: 6   LLTPLLLSCFFLSLSQALVPPDQTFKFVNEGDFGDFAVEYGANYRVLSIAQS-----PFQ 65
           L+T L +S   +S+  A VPP++ F+ +NE  +  +  EY A+YR L+         PFQ
Sbjct: 8   LITALAIST--VSVVMAQVPPEKQFRVLNEPGYAPYITEYDASYRFLNSPNQNFFTIPFQ 67

Query: 66  LAFYNTTPNAYTLALRMAILRSESAMRWVWEANRGRPVRDNATFSLGADGNLVLADSDGS 125
           L FYNTTP+AY LALR+   R  S  RW+W+ANR  PV DN+T S G +GNLVLA+ +G 
Sbjct: 68  LMFYNTTPSAYVLALRVGTRRDMSFTRWIWDANRNNPVGDNSTLSFGRNGNLVLAELNGQ 127

Query: 126 VVWQSNTANKGVVGFKLLPNGNMVLHDSSGKFLWQSFDSPTDTLLVGQSLRLGGAIKLVS 185
           V WQ+NTANKGV GF++LPNGNMVLHD  GKF+WQSFD PTDTLLVGQSL++ G  KLVS
Sbjct: 128 VKWQTNTANKGVTGFQILPNGNMVLHDKHGKFVWQSFDHPTDTLLVGQSLKVNGVNKLVS 187

Query: 186 RASEKLNSNGPYSFVMEKKTLNLYYKSPNSPKPMRYFTSSNRFTIQKGSLSKVTLNADVD 245
           R S+   S+GPYS V++ K L +Y     +P     +T  +     +G+++        +
Sbjct: 188 RTSDMNGSDGPYSMVLDNKGLTMYVNKTGTPLVYGGWTDHD----FRGTVTFAVTREFDN 247

Query: 246 PDQGFATEIKLDYESAGST---------------ESGGTI-VARPKYNATLTFLRLGIDG 305
             +  A E+ L+     +T                 GGT+ + +  YN T+++LRLG DG
Sbjct: 248 LTEPSAYELLLEPAPQPATNPGNNRRLLQVRPIGSGGGTLNLNKINYNGTISYLRLGSDG 307

Query: 306 NLRLITYNDKVDWGPSEVSFTLFDRDSNWESECQWPERCGQLGLCEENQCVGCPTANGVV 365
           +L+  +Y     +   E +F  F   + +  +C  P  CG  G C+   CVGCPT  G++
Sbjct: 308 SLKAFSYFPAATYLEWEETFAFF--SNYFVRQCGLPTFCGDYGYCDRGMCVGCPTPKGLL 367

Query: 366 GWSKSCAAEK-VRSCD---PKSFHYYKIEGVDHFLAKY-NRGEGPMGQRECEKKCSLDCK 425
            WS  CA  K  + C     K+ +YYKI GV+HF   Y N G+GP    +C+ KC  DCK
Sbjct: 368 AWSDKCAPPKTTQFCSGGKGKAVNYYKIVGVEHFTGPYVNDGQGPTSVNDCKAKCDRDCK 427

Query: 426 CLGYFYQTKGSLCWVANELKTLIKVANSTHLGFIK 435
           CLGYFY+ K   C +A  L TLIK AN++ + +IK
Sbjct: 428 CLGYFYKEKDKKCLLAPLLGTLIKDANTSSVAYIK 454

BLAST of MC10g_new0157 vs. NCBI nr
Match: XP_038898869.1 (epidermis-specific secreted glycoprotein EP1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 774 bits (1998), Expect = 1.88e-280
Identity = 367/436 (84.17%), Postives = 403/436 (92.43%), Query Frame = 0

Query: 4   PPLLTPLLLSCFFL--SLSQALVPPDQTFKFVNEGDFGDFAVEYGANYRVLSIAQSPFQL 63
           P LLTPLLLS FFL  SLS ALVPP++TF+FVNEGDFGDFAVEY A YR LSI+ SPFQL
Sbjct: 3   PTLLTPLLLSFFFLFISLSLALVPPNETFRFVNEGDFGDFAVEYDATYRPLSISNSPFQL 62

Query: 64  AFYNTTPNAYTLALRMAILRSESAMRWVWEANRGRPVRDNATFSLGADGNLVLADSDGSV 123
            FYNTTPNAYTLALRMAILRSESA RWVWEANRGRPVR+NATFSLGADGNL+LA++DG+V
Sbjct: 63  MFYNTTPNAYTLALRMAILRSESAKRWVWEANRGRPVRENATFSLGADGNLILAEADGTV 122

Query: 124 VWQSNTANKGVVGFKLLPNGNMVLHDSSGKFLWQSFDSPTDTLLVGQSLRLGGAIKLVSR 183
           VWQSNTANKGVV  +LLPNGNMVL DS+GKF+WQSFDSPTDTLLVGQSLRLGGA+KLVSR
Sbjct: 123 VWQSNTANKGVVRLELLPNGNMVLIDSNGKFVWQSFDSPTDTLLVGQSLRLGGAMKLVSR 182

Query: 184 ASEKLNSNGPYSFVMEKKTLNLYYKSPNSPKPMRYFTSSNRFTIQKGSLSKVTLNADVDP 243
           ASEK N NGPYSFVME+K L LYYKSPNSPKPMRYF SSN FTIQKG+LS+VTLNA+V+P
Sbjct: 183 ASEKSNVNGPYSFVMERKALALYYKSPNSPKPMRYFASSNWFTIQKGTLSRVTLNAEVEP 242

Query: 244 DQGFATEIKLDYESAGSTESGGTIVARPKYNATLTFLRLGIDGNLRLITYNDKVDWGPSE 303
           DQGFATE+ L+YE AGSTE+GGTI+ RPKYN+TLTFLRLGIDGN+RL TYNDKVDWGPSE
Sbjct: 243 DQGFATELNLNYEVAGSTETGGTILTRPKYNSTLTFLRLGIDGNVRLFTYNDKVDWGPSE 302

Query: 304 VSFTLFDRDSNWESECQWPERCGQLGLCEENQCVGCPTANGVVGWSKSCAAEKVRSCDPK 363
           +SFTLFDR+SN E+ECQWPERCGQLG+CEENQCV CPT  G++GWSK C A+KV SCDPK
Sbjct: 303 ISFTLFDRESNTENECQWPERCGQLGVCEENQCVACPTEKGLLGWSKMCMAKKVSSCDPK 362

Query: 364 SFHYYKIEGVDHFLAKYNRGEGPMGQRECEKKCSLDCKCLGYFYQTKGSLCWVANELKTL 423
           SFHYYK+EGVDHFL KYN+GEGPMGQ+ECEKKC+LDCKCLGYFYQT+GSLCWVANELKTL
Sbjct: 363 SFHYYKLEGVDHFLTKYNKGEGPMGQKECEKKCNLDCKCLGYFYQTRGSLCWVANELKTL 422

Query: 424 IKVANSTHLGFIKTPN 437
           IKV NSTHLGFIKTPN
Sbjct: 423 IKVDNSTHLGFIKTPN 438

BLAST of MC10g_new0157 vs. NCBI nr
Match: XP_038880567.1 (epidermis-specific secreted glycoprotein EP1-like [Benincasa hispida])

HSP 1 Score: 773 bits (1995), Expect = 5.38e-280
Identity = 367/436 (84.17%), Postives = 402/436 (92.20%), Query Frame = 0

Query: 4   PPLLTPLLLSC--FFLSLSQALVPPDQTFKFVNEGDFGDFAVEYGANYRVLSIAQSPFQL 63
           PPLLTPLLLS   FF+SLS ALVPP++TF+FVNEGDFG F VEY A YR LSI+ SPFQL
Sbjct: 3   PPLLTPLLLSFLFFFISLSLALVPPNETFRFVNEGDFGVFIVEYDATYRPLSISNSPFQL 62

Query: 64  AFYNTTPNAYTLALRMAILRSESAMRWVWEANRGRPVRDNATFSLGADGNLVLADSDGSV 123
            FYNTTPNAYTLALRMAILRSESAMRWVWEANRG PVR+NATFSLGADGNLVLA+SDG+V
Sbjct: 63  MFYNTTPNAYTLALRMAILRSESAMRWVWEANRGHPVRENATFSLGADGNLVLAESDGTV 122

Query: 124 VWQSNTANKGVVGFKLLPNGNMVLHDSSGKFLWQSFDSPTDTLLVGQSLRLGGAIKLVSR 183
           VWQSNTANKGVV  +LLPNGNMVL DS+GKF+WQSFDSPTDTLLVGQSLRLGGA+KLVSR
Sbjct: 123 VWQSNTANKGVVRLELLPNGNMVLLDSNGKFVWQSFDSPTDTLLVGQSLRLGGAMKLVSR 182

Query: 184 ASEKLNSNGPYSFVMEKKTLNLYYKSPNSPKPMRYFTSSNRFTIQKGSLSKVTLNADVDP 243
           ASEK N NGPYSFVME+K L LYYKSPNSPKPMRYF SSN FTIQKG+LS+VTLNA+V+P
Sbjct: 183 ASEKSNVNGPYSFVMERKALALYYKSPNSPKPMRYFASSNWFTIQKGTLSRVTLNAEVEP 242

Query: 244 DQGFATEIKLDYESAGSTESGGTIVARPKYNATLTFLRLGIDGNLRLITYNDKVDWGPSE 303
           DQGFATE+ L+YE AGSTE+GGTI+ RPKYN+TLTFLRLGIDGN+RL TYNDKVDWGPSE
Sbjct: 243 DQGFATELNLNYEVAGSTETGGTILTRPKYNSTLTFLRLGIDGNVRLFTYNDKVDWGPSE 302

Query: 304 VSFTLFDRDSNWESECQWPERCGQLGLCEENQCVGCPTANGVVGWSKSCAAEKVRSCDPK 363
           +SFTLFDR+SN E+ECQWPERCGQLG+CEENQCV CPT  G++GWSK C A+KV SCDPK
Sbjct: 303 ISFTLFDRESNTENECQWPERCGQLGVCEENQCVACPTEKGLLGWSKMCMAKKVSSCDPK 362

Query: 364 SFHYYKIEGVDHFLAKYNRGEGPMGQRECEKKCSLDCKCLGYFYQTKGSLCWVANELKTL 423
           SFHYYK+EGVDHFL KYN+GEGPMGQ+ECEKKC+LDCKCLGYFYQT+GSLCWVANELKTL
Sbjct: 363 SFHYYKLEGVDHFLTKYNKGEGPMGQKECEKKCNLDCKCLGYFYQTRGSLCWVANELKTL 422

Query: 424 IKVANSTHLGFIKTPN 437
           IKV NSTHLGFIKTPN
Sbjct: 423 IKVDNSTHLGFIKTPN 438

BLAST of MC10g_new0157 vs. NCBI nr
Match: XP_038898943.1 (epidermis-specific secreted glycoprotein EP1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 768 bits (1982), Expect = 5.14e-278
Identity = 364/436 (83.49%), Postives = 401/436 (91.97%), Query Frame = 0

Query: 4   PPLLTPLLLSCFFL--SLSQALVPPDQTFKFVNEGDFGDFAVEYGANYRVLSIAQSPFQL 63
           P LLTPLLLS FFL  SLS ALVPP++TF+FVNEGDFGDFAVEY A YR LSI+ SPFQL
Sbjct: 3   PTLLTPLLLSFFFLFISLSLALVPPNETFRFVNEGDFGDFAVEYDATYRPLSISNSPFQL 62

Query: 64  AFYNTTPNAYTLALRMAILRSESAMRWVWEANRGRPVRDNATFSLGADGNLVLADSDGSV 123
            FYNTTPNAYTLALRMAILRSESA RWVWEANRGRPVR+NATFSLGADGNL+LA++DG+V
Sbjct: 63  MFYNTTPNAYTLALRMAILRSESAKRWVWEANRGRPVRENATFSLGADGNLILAEADGTV 122

Query: 124 VWQSNTANKGVVGFKLLPNGNMVLHDSSGKFLWQSFDSPTDTLLVGQSLRLGGAIKLVSR 183
           VWQSNTANKGVV  +LLPNGNMVL DS+GKF+WQSFD PTDTLLVGQSLRLGGA+KLVSR
Sbjct: 123 VWQSNTANKGVVRLELLPNGNMVLIDSNGKFVWQSFDFPTDTLLVGQSLRLGGAMKLVSR 182

Query: 184 ASEKLNSNGPYSFVMEKKTLNLYYKSPNSPKPMRYFTSSNRFTIQKGSLSKVTLNADVDP 243
           ASEK N NGPYSFVME+K L LY+KSPNSPKPMRYF SSN FTI+KG+LS+VTLNA+VDP
Sbjct: 183 ASEKSNVNGPYSFVMERKALALYFKSPNSPKPMRYFASSNWFTIRKGTLSRVTLNAEVDP 242

Query: 244 DQGFATEIKLDYESAGSTESGGTIVARPKYNATLTFLRLGIDGNLRLITYNDKVDWGPSE 303
           DQGFATE+ L+YE AGSTE+G TI+ RPKYN+TLTFLRLGIDGN+RL TYNDKVDWGPSE
Sbjct: 243 DQGFATELNLNYEVAGSTEAGSTILTRPKYNSTLTFLRLGIDGNVRLFTYNDKVDWGPSE 302

Query: 304 VSFTLFDRDSNWESECQWPERCGQLGLCEENQCVGCPTANGVVGWSKSCAAEKVRSCDPK 363
           +SFTLFDR+SN E+ECQWPERCGQLG+CEENQCV CPT  G++GWSK C A+KV SCDPK
Sbjct: 303 ISFTLFDRESNTENECQWPERCGQLGVCEENQCVACPTEKGLLGWSKMCMAKKVSSCDPK 362

Query: 364 SFHYYKIEGVDHFLAKYNRGEGPMGQRECEKKCSLDCKCLGYFYQTKGSLCWVANELKTL 423
           SFHYYK+EGVDHFL KYN+GEGPMGQ+ECEKKC+LDCKCLGYFYQT+GSLCWVANELKTL
Sbjct: 363 SFHYYKLEGVDHFLTKYNKGEGPMGQKECEKKCNLDCKCLGYFYQTRGSLCWVANELKTL 422

Query: 424 IKVANSTHLGFIKTPN 437
           IKV NSTHLGFIKTPN
Sbjct: 423 IKVDNSTHLGFIKTPN 438

BLAST of MC10g_new0157 vs. NCBI nr
Match: XP_038899017.1 (epidermis-specific secreted glycoprotein EP1-like isoform X3 [Benincasa hispida])

HSP 1 Score: 767 bits (1980), Expect = 1.08e-277
Identity = 366/437 (83.75%), Postives = 402/437 (91.99%), Query Frame = 0

Query: 4   PPLLTPLLLSCFFL--SLSQALVPPDQTFKFVNEGDFGDFAVEYGANYRVLSIAQSPFQL 63
           P LLTPLLLS FFL  SLS ALVPP++TF+FVNEGDFGDFAVEY A YR LSI+ SPFQL
Sbjct: 3   PTLLTPLLLSFFFLFISLSLALVPPNETFRFVNEGDFGDFAVEYDATYRPLSISNSPFQL 62

Query: 64  AFYNTTPNAYTLALRMAILRSESAMRWVWEANRGRPVRDNATFSLGADGNLVLADSDGSV 123
            FYNTTPNAYTLALRMAILRSESA RWVWEANRGRPVR+NATFSLGADGNL+LA++DG+V
Sbjct: 63  MFYNTTPNAYTLALRMAILRSESAKRWVWEANRGRPVRENATFSLGADGNLILAEADGTV 122

Query: 124 VWQSNTANKGVVGFKLLPNGNMVLHDSSGKFLWQSFDSPTDTLLVGQSLRLGGAIKL-VS 183
           VWQSNTANKGVV  +LLPNGNMVL DS+GKF+WQSFD PTDTLLVGQSLRLGGA+KL VS
Sbjct: 123 VWQSNTANKGVVRLELLPNGNMVLIDSNGKFVWQSFDFPTDTLLVGQSLRLGGAMKLLVS 182

Query: 184 RASEKLNSNGPYSFVMEKKTLNLYYKSPNSPKPMRYFTSSNRFTIQKGSLSKVTLNADVD 243
           RASEK N NGPYSFVME+K L LYYKSPNSPKPMRYF SSN FTIQKG+LS+VTLNA+V+
Sbjct: 183 RASEKSNVNGPYSFVMERKALALYYKSPNSPKPMRYFASSNWFTIQKGTLSRVTLNAEVE 242

Query: 244 PDQGFATEIKLDYESAGSTESGGTIVARPKYNATLTFLRLGIDGNLRLITYNDKVDWGPS 303
           PDQGFATE+ L+YE AGSTE+GGTI+ RPKYN+TLTFLRLGIDGN+RL TYNDKVDWGPS
Sbjct: 243 PDQGFATELNLNYEVAGSTETGGTILTRPKYNSTLTFLRLGIDGNVRLFTYNDKVDWGPS 302

Query: 304 EVSFTLFDRDSNWESECQWPERCGQLGLCEENQCVGCPTANGVVGWSKSCAAEKVRSCDP 363
           E+SFTLFDR+SN E+ECQWPERCGQLG+CEENQCV CPT  G++GWSK C A+KV SCDP
Sbjct: 303 EISFTLFDRESNTENECQWPERCGQLGVCEENQCVACPTEKGLLGWSKMCMAKKVSSCDP 362

Query: 364 KSFHYYKIEGVDHFLAKYNRGEGPMGQRECEKKCSLDCKCLGYFYQTKGSLCWVANELKT 423
           KSFHYYK+EGVDHFL KYN+GEGPMGQ+ECEKKC+LDCKCLGYFYQT+GSLCWVANELKT
Sbjct: 363 KSFHYYKLEGVDHFLTKYNKGEGPMGQKECEKKCNLDCKCLGYFYQTRGSLCWVANELKT 422

Query: 424 LIKVANSTHLGFIKTPN 437
           LIKV NSTHLGFIKTPN
Sbjct: 423 LIKVDNSTHLGFIKTPN 439

BLAST of MC10g_new0157 vs. NCBI nr
Match: XP_023551867.1 (epidermis-specific secreted glycoprotein EP1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 764 bits (1972), Expect = 1.72e-276
Identity = 361/436 (82.80%), Postives = 399/436 (91.51%), Query Frame = 0

Query: 5   PLLTPLLLSCFFL--SLSQALVPPDQTFKFVNEGDFGDFAVEYGANYRVLSIAQSPFQLA 64
           PLLTPLL+S FFL  S S ALVP ++TFKFVNEGDFG+F +EY   YRVLSI + PFQLA
Sbjct: 4   PLLTPLLISLFFLFFSFSLALVPANETFKFVNEGDFGEFIIEYDGTYRVLSIFRFPFQLA 63

Query: 65  FYNTTPNAYTLALRMAILRSESAMRWVWEANRGRPVRDNATFSLGADGNLVLADSDGSVV 124
           FYNTTPNAYTLALR++I RSESA+RWVWEANRGRPVR+NATFSL A+GNLVLA++DG+VV
Sbjct: 64  FYNTTPNAYTLALRISIRRSESAIRWVWEANRGRPVRENATFSLSANGNLVLAEADGTVV 123

Query: 125 WQSNTANKGVVGFKLLPNGNMVLHDSSGKFLWQSFDSPTDTLLVGQSLRLGGAIKLVSRA 184
           WQSNTANKGVVGF+LLP+GNMVL DS+GKFLWQSFDSPTDTLLVGQSLRLGG  KLVSRA
Sbjct: 124 WQSNTANKGVVGFELLPSGNMVLLDSNGKFLWQSFDSPTDTLLVGQSLRLGGPTKLVSRA 183

Query: 185 SEKLNSNGPYSFVMEKKTLNLYYKSPNSPKPMRYFTSSNRFTIQKGSLSKVTLNADVDPD 244
           SE +N NGPYS VME+K L+LYYKSPNSPKPMRYFTSS+ FTIQKG+LSK+TLNA V+PD
Sbjct: 184 SEVMNVNGPYSLVMERKALSLYYKSPNSPKPMRYFTSSDWFTIQKGTLSKITLNAAVEPD 243

Query: 245 QGFATEIKLDYESAGSTESGGTIVARPKYNATLTFLRLGIDGNLRLITYNDKVDWGPSEV 304
           QGFATE+ L+YE +GS ESGG I+ RPKYN+TLTFLRLGIDGNLRLITYNDKVDWGPSE+
Sbjct: 244 QGFATELTLNYEVSGSMESGGPILTRPKYNSTLTFLRLGIDGNLRLITYNDKVDWGPSEI 303

Query: 305 SFTLFDRDSNWESECQWPERCGQLGLCEENQCVGCPTANGVVGWSKSCAAEKVRSCDPKS 364
           SFTLFDRDS WE+ECQWPERCGQ GLCE+NQCV CPT NG+ GWSKSCA +KV SCDPKS
Sbjct: 304 SFTLFDRDSTWENECQWPERCGQFGLCEDNQCVACPTENGLAGWSKSCAPKKVSSCDPKS 363

Query: 365 FHYYKIEGVDHFLAKYNRGEGPMGQRECEKKCSLDCKCLGYFYQTKGSLCWVANELKTLI 424
           FHYYK+ GVDHFL KYN+GEGPMGQ+ECEKKC+LDCKCLGYFYQTKGSLCWVANELKTLI
Sbjct: 364 FHYYKLVGVDHFLTKYNKGEGPMGQKECEKKCNLDCKCLGYFYQTKGSLCWVANELKTLI 423

Query: 425 KVANSTHLGFIKTPNK 438
           KVANSTHLGFIKTPNK
Sbjct: 424 KVANSTHLGFIKTPNK 439

BLAST of MC10g_new0157 vs. ExPASy TrEMBL
Match: A0A6J1ETQ8 (epidermis-specific secreted glycoprotein EP1-like OS=Cucurbita moschata OX=3662 GN=LOC111437646 PE=4 SV=1)

HSP 1 Score: 757 bits (1955), Expect = 3.11e-274
Identity = 356/436 (81.65%), Postives = 402/436 (92.20%), Query Frame = 0

Query: 5   PLLTPLLLSCFFL--SLSQALVPPDQTFKFVNEGDFGDFAVEYGANYRVLSIAQSPFQLA 64
           PLLTPLL+S FFL  S S ALVP ++TFKFVNEG+FGDFAVEYG  YRVLSI + PFQLA
Sbjct: 4   PLLTPLLISFFFLFFSFSLALVPANETFKFVNEGEFGDFAVEYGGTYRVLSIFRFPFQLA 63

Query: 65  FYNTTPNAYTLALRMAILRSESAMRWVWEANRGRPVRDNATFSLGADGNLVLADSDGSVV 124
           FYNTTPNAYTLALR++I RSESA+RWVWEANRGRPVR+NATFSL A+GNLVLA++DG+VV
Sbjct: 64  FYNTTPNAYTLALRISIRRSESAIRWVWEANRGRPVRENATFSLSANGNLVLAEADGTVV 123

Query: 125 WQSNTANKGVVGFKLLPNGNMVLHDSSGKFLWQSFDSPTDTLLVGQSLRLGGAIKLVSRA 184
           WQSNTANKGVVGF+LLP+GNMVL DS+GKFLWQSFDSPTDTLLVGQSLRLGG +KLVSRA
Sbjct: 124 WQSNTANKGVVGFELLPSGNMVLFDSNGKFLWQSFDSPTDTLLVGQSLRLGGPMKLVSRA 183

Query: 185 SEKLNSNGPYSFVMEKKTLNLYYKSPNSPKPMRYFTSSNRFTIQKGSLSKVTLNADVDPD 244
           SE++N NGPYS VME+K L+LYYKSPNSPKPMRY++S++  +++KG L+ +TLNA VDPD
Sbjct: 184 SEEMNVNGPYSLVMERKVLSLYYKSPNSPKPMRYYSSTDMLSVRKGVLANITLNAAVDPD 243

Query: 245 QGFATEIKLDYESAGSTESGGTIVARPKYNATLTFLRLGIDGNLRLITYNDKVDWGPSEV 304
           QGFATE+ L+Y++ GSTESGG I+ RPKYN+TLTFLRLGIDGNLRLITYNDKVDWGPSE+
Sbjct: 244 QGFATELTLNYDT-GSTESGGPILTRPKYNSTLTFLRLGIDGNLRLITYNDKVDWGPSEI 303

Query: 305 SFTLFDRDSNWESECQWPERCGQLGLCEENQCVGCPTANGVVGWSKSCAAEKVRSCDPKS 364
           SFTLFDRDS+WE+ECQWPERCGQ GLCE+NQCV CPT NG+ GWSKSCA +KV SCDPKS
Sbjct: 304 SFTLFDRDSSWENECQWPERCGQFGLCEDNQCVACPTENGLAGWSKSCAPKKVSSCDPKS 363

Query: 365 FHYYKIEGVDHFLAKYNRGEGPMGQRECEKKCSLDCKCLGYFYQTKGSLCWVANELKTLI 424
           FHYYK+ GVDHFL KYN+GEGPMGQ+ECEKKC+LDCKCLGYFYQTKGSLCWVANELKTLI
Sbjct: 364 FHYYKLVGVDHFLTKYNKGEGPMGQKECEKKCNLDCKCLGYFYQTKGSLCWVANELKTLI 423

Query: 425 KVANSTHLGFIKTPNK 438
           KVANSTHLGFIKTPNK
Sbjct: 424 KVANSTHLGFIKTPNK 438

BLAST of MC10g_new0157 vs. ExPASy TrEMBL
Match: A0A6J1JA71 (epidermis-specific secreted glycoprotein EP1-like OS=Cucurbita maxima OX=3661 GN=LOC111482665 PE=4 SV=1)

HSP 1 Score: 742 bits (1915), Expect = 3.85e-268
Identity = 348/436 (79.82%), Postives = 394/436 (90.37%), Query Frame = 0

Query: 5   PLLTPLLLSCFFL--SLSQALVPPDQTFKFVNEGDFGDFAVEYGANYRVLSIAQSPFQLA 64
           PLLTP L+S FFL  S S ALVP ++TFKFVNEG+FG+F +EY   YR LSI + PFQL+
Sbjct: 4   PLLTPFLISFFFLFFSFSLALVPANETFKFVNEGEFGEFIIEYDGTYRPLSIFRFPFQLS 63

Query: 65  FYNTTPNAYTLALRMAILRSESAMRWVWEANRGRPVRDNATFSLGADGNLVLADSDGSVV 124
           FYNTTPNAYTLALR++I RSESA+RWVWEANRGRPVR+NATFSL  +GNLVLA++DG+VV
Sbjct: 64  FYNTTPNAYTLALRISIRRSESAIRWVWEANRGRPVRENATFSLSTNGNLVLAEADGTVV 123

Query: 125 WQSNTANKGVVGFKLLPNGNMVLHDSSGKFLWQSFDSPTDTLLVGQSLRLGGAIKLVSRA 184
           WQSNTANKGVVGF+LLP+GNMVL DS+GKFLWQSFDSPTDTLLVGQSLRLGG  KLVSRA
Sbjct: 124 WQSNTANKGVVGFELLPSGNMVLFDSNGKFLWQSFDSPTDTLLVGQSLRLGGPTKLVSRA 183

Query: 185 SEKLNSNGPYSFVMEKKTLNLYYKSPNSPKPMRYFTSSNRFTIQKGSLSKVTLNADVDPD 244
           SE +N NGPYS VME+K L+LYYKSPNSPKPMRY++S++  +++KG L+ +TLNA VDPD
Sbjct: 184 SEVMNVNGPYSLVMERKALSLYYKSPNSPKPMRYYSSTDMLSVRKGVLANITLNAAVDPD 243

Query: 245 QGFATEIKLDYESAGSTESGGTIVARPKYNATLTFLRLGIDGNLRLITYNDKVDWGPSEV 304
           QGFATE+ L+YE+ GSTESGG I+ RPKYN+TLTFLRLGIDGNLRLITYNDKVDWGPSE+
Sbjct: 244 QGFATELTLNYET-GSTESGGPILTRPKYNSTLTFLRLGIDGNLRLITYNDKVDWGPSEI 303

Query: 305 SFTLFDRDSNWESECQWPERCGQLGLCEENQCVGCPTANGVVGWSKSCAAEKVRSCDPKS 364
           SFTLFDRDS WE+ECQWPERCGQ GLCE+NQCV CPT NG+ GWSK+CA +KV SCDPKS
Sbjct: 304 SFTLFDRDSTWENECQWPERCGQFGLCEDNQCVACPTENGLAGWSKTCAPKKVSSCDPKS 363

Query: 365 FHYYKIEGVDHFLAKYNRGEGPMGQRECEKKCSLDCKCLGYFYQTKGSLCWVANELKTLI 424
           FHYYK+ GVDHFL KYN+GEGPMGQ+ECEKKC+LDCKCLGYFYQTKGSLCWVANELKTLI
Sbjct: 364 FHYYKLVGVDHFLTKYNKGEGPMGQKECEKKCNLDCKCLGYFYQTKGSLCWVANELKTLI 423

Query: 425 KVANSTHLGFIKTPNK 438
           KVANSTHLGFIKTPNK
Sbjct: 424 KVANSTHLGFIKTPNK 438

BLAST of MC10g_new0157 vs. ExPASy TrEMBL
Match: A0A0A0L0B9 (Bulb-type lectin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G082380 PE=4 SV=1)

HSP 1 Score: 733 bits (1891), Expect = 1.87e-264
Identity = 355/440 (80.68%), Postives = 393/440 (89.32%), Query Frame = 0

Query: 4   PPLLTPLLLSCFFL-SLSQALVPPDQTFKFVNEGDFGDFAVEYGANYRVLSIAQSPFQLA 63
           PPLLTPLLLS FF  SLS A+VP ++TFKFVNEGDFGDFAVEY   YR LSI+ SPFQL 
Sbjct: 3   PPLLTPLLLSFFFFFSLSFAIVPSNETFKFVNEGDFGDFAVEYDGTYRPLSISNSPFQLM 62

Query: 64  FYNTTPNAYTLALRMAILRSESAMRWVWEANRGRPVRDNATFSLGADGNLVLADSDGSVV 123
           FYNTTPNAYTLALRMAILRSESA RWVWEANRGRPVR+NAT SLG+DGNLVLA++DG+VV
Sbjct: 63  FYNTTPNAYTLALRMAILRSESAKRWVWEANRGRPVRENATLSLGSDGNLVLAEADGTVV 122

Query: 124 WQSNTANKGVVGFKLLPNGNMVLHDSSGKFLWQSFDSPTDTLLVGQSLRLGGAIKLVSRA 183
           WQ+NTANKGVV   LLPNGNMVL DS+GKF+WQSFDSPTDTLLVGQSLR+GG  KLVSRA
Sbjct: 123 WQTNTANKGVVKLDLLPNGNMVLLDSNGKFVWQSFDSPTDTLLVGQSLRIGGVTKLVSRA 182

Query: 184 SEKLNSNGPYSFVMEKKTLNLYYKSPNSPKPMRYFT-SSNRFTIQKGSLSKVTLNADVDP 243
           SEKLN NGPYSFVME+  ++LYYKSPNSPKPMRYF  SSN FTIQKGSL++VTL A+VDP
Sbjct: 183 SEKLNVNGPYSFVMERNAMSLYYKSPNSPKPMRYFAGSSNWFTIQKGSLARVTLRAEVDP 242

Query: 244 DQGFATEIKLDYESAGSTESGGTIVARPKYNATLTFLRLGIDGNLRLITYNDKVDWGPSE 303
           DQGFATE+ L+YE AG TE+GG I++RPKYN+TLTFLRLGIDGNLRL TYNDKVDW PSE
Sbjct: 243 DQGFATELTLNYEVAG-TENGGPILSRPKYNSTLTFLRLGIDGNLRLFTYNDKVDWSPSE 302

Query: 304 VSFTLFDRD---SNWESECQWPERCGQLGLCEENQCVGCPTANGVVGWSKSCAAEKVRSC 363
           ++FTLFDR+    N ESECQWPERCGQ GLCEENQCV CPT  G++GWSK+C A+KV SC
Sbjct: 303 ITFTLFDREFNTGNTESECQWPERCGQFGLCEENQCVACPTEKGLLGWSKTCMAKKVSSC 362

Query: 364 DPKSFHYYKIEGVDHFLAKYNRGEGPMGQRECEKKCSLDCKCLGYFYQTKGSLCWVANEL 423
           DPKSFHYYK+EGVDHFL KYN+GEG + Q++CEKKC+LDCKCLGYFYQTKGSLCWVANEL
Sbjct: 363 DPKSFHYYKVEGVDHFLTKYNKGEG-LRQKDCEKKCNLDCKCLGYFYQTKGSLCWVANEL 422

Query: 424 KTLIKVANSTHLGFIKTPNK 438
           KTLIKV NSTHLGFIKTPNK
Sbjct: 423 KTLIKVDNSTHLGFIKTPNK 440

BLAST of MC10g_new0157 vs. ExPASy TrEMBL
Match: A0A5A7V4R1 (Epidermis-specific secreted glycoprotein EP1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold468G001550 PE=4 SV=1)

HSP 1 Score: 731 bits (1888), Expect = 5.36e-264
Identity = 354/440 (80.45%), Postives = 394/440 (89.55%), Query Frame = 0

Query: 4   PPLLTPLLLSCFFLS-LSQALVPPDQTFKFVNEGDFGDFAVEYGANYRVLSIAQSPFQLA 63
           PPLLTPLLLS FF S LS A+VPP++TFKFVNEGDFGDFAVEY   YR LSI+ SPFQL 
Sbjct: 3   PPLLTPLLLSFFFFSSLSFAIVPPNETFKFVNEGDFGDFAVEYDGTYRPLSISNSPFQLM 62

Query: 64  FYNTTPNAYTLALRMAILRSESAMRWVWEANRGRPVRDNATFSLGADGNLVLADSDGSVV 123
           FYNTTPNAYTLALRMAILRSESA RWVWEANRGRPVR+NAT SLG+DGNLVLA++DG+VV
Sbjct: 63  FYNTTPNAYTLALRMAILRSESAKRWVWEANRGRPVRENATLSLGSDGNLVLAEADGTVV 122

Query: 124 WQSNTANKGVVGFKLLPNGNMVLHDSSGKFLWQSFDSPTDTLLVGQSLRLGGAIKLVSRA 183
           WQ+NTANKGVV   LLPNGNMVL DS+GKF+WQSFDSPTDTLLVGQSLRLGG  KLVSRA
Sbjct: 123 WQTNTANKGVVKLDLLPNGNMVLLDSNGKFVWQSFDSPTDTLLVGQSLRLGGVTKLVSRA 182

Query: 184 SEKLNSNGPYSFVMEKKTLNLYYKSPNSPKPMRYFT-SSNRFTIQKGSLSKVTLNADVDP 243
           SEKLN NGPYSFVME+K ++LYYKSPNSPKPMRYF  SSN FTIQKG+L++VTL A+VDP
Sbjct: 183 SEKLNVNGPYSFVMERKAVSLYYKSPNSPKPMRYFAGSSNWFTIQKGTLARVTLRAEVDP 242

Query: 244 DQGFATEIKLDYESAGSTESGGTIVARPKYNATLTFLRLGIDGNLRLITYNDKVDWGPSE 303
            QGFATE+ L+YE AG TE+GG I++RPKYN+TLTFLRLGIDGNLRL TYND+VDW PSE
Sbjct: 243 GQGFATELTLNYEVAG-TENGGPILSRPKYNSTLTFLRLGIDGNLRLFTYNDQVDWSPSE 302

Query: 304 VSFTLFDRD---SNWESECQWPERCGQLGLCEENQCVGCPTANGVVGWSKSCAAEKVRSC 363
           ++FTLFDR+    N ESECQWPERCGQ GLCEENQCV CPT  G++GWSK+C A+KV SC
Sbjct: 303 ITFTLFDREFNTGNTESECQWPERCGQFGLCEENQCVACPTEKGLLGWSKTCMAKKVSSC 362

Query: 364 DPKSFHYYKIEGVDHFLAKYNRGEGPMGQRECEKKCSLDCKCLGYFYQTKGSLCWVANEL 423
           DPKSFHYYK+EGVDHFL K+N+GEG + Q++CEKKC+LDCKCLGYFYQTKGSLCWVANEL
Sbjct: 363 DPKSFHYYKLEGVDHFLTKFNKGEG-LSQKDCEKKCNLDCKCLGYFYQTKGSLCWVANEL 422

Query: 424 KTLIKVANSTHLGFIKTPNK 438
           KTLIKV NSTHLGFIKTPNK
Sbjct: 423 KTLIKVDNSTHLGFIKTPNK 440

BLAST of MC10g_new0157 vs. ExPASy TrEMBL
Match: A0A5D3CWN6 (Epidermis-specific secreted glycoprotein EP1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G00840 PE=4 SV=1)

HSP 1 Score: 729 bits (1881), Expect = 6.25e-263
Identity = 352/440 (80.00%), Postives = 393/440 (89.32%), Query Frame = 0

Query: 4   PPLLTPLLLSCFFLS-LSQALVPPDQTFKFVNEGDFGDFAVEYGANYRVLSIAQSPFQLA 63
           PPLLTPLLLS FF S LS A+VPP++TFKFVNEGDFGDFAVEY   YR LSI+ SPFQL 
Sbjct: 3   PPLLTPLLLSFFFFSSLSFAIVPPNETFKFVNEGDFGDFAVEYDGTYRPLSISNSPFQLM 62

Query: 64  FYNTTPNAYTLALRMAILRSESAMRWVWEANRGRPVRDNATFSLGADGNLVLADSDGSVV 123
           FYNTTPNAYTLALRMAILRSESA RWVWEANRGRPVR+NAT SLG+DGNLVLA++DG+VV
Sbjct: 63  FYNTTPNAYTLALRMAILRSESAKRWVWEANRGRPVRENATLSLGSDGNLVLAEADGTVV 122

Query: 124 WQSNTANKGVVGFKLLPNGNMVLHDSSGKFLWQSFDSPTDTLLVGQSLRLGGAIKLVSRA 183
           WQ+NTANKGVV   LLPNGNMVL DS+GKF+WQSFDSPTDTLLVGQSLR+GG  KLVSRA
Sbjct: 123 WQTNTANKGVVKLDLLPNGNMVLLDSNGKFVWQSFDSPTDTLLVGQSLRIGGVTKLVSRA 182

Query: 184 SEKLNSNGPYSFVMEKKTLNLYYKSPNSPKPMRYFT-SSNRFTIQKGSLSKVTLNADVDP 243
           SEKLN NGPYSFVME+K ++LYYKSPNSPKPMRYF  SSN FTIQKG+L++VTL A+VDP
Sbjct: 183 SEKLNVNGPYSFVMERKAVSLYYKSPNSPKPMRYFAGSSNWFTIQKGTLARVTLRAEVDP 242

Query: 244 DQGFATEIKLDYESAGSTESGGTIVARPKYNATLTFLRLGIDGNLRLITYNDKVDWGPSE 303
            QGFATE+ L+YE AG TE+GG I++RPKYN+TLTFLRLGIDGNLRL TYND+VDW PSE
Sbjct: 243 GQGFATELTLNYEVAG-TENGGPILSRPKYNSTLTFLRLGIDGNLRLFTYNDQVDWSPSE 302

Query: 304 VSFTLFDRD---SNWESECQWPERCGQLGLCEENQCVGCPTANGVVGWSKSCAAEKVRSC 363
           ++FTLFDR+    N ESECQWPERCGQ GLCEENQCV CPT  G++GWSK+C A+KV SC
Sbjct: 303 ITFTLFDREFNTGNTESECQWPERCGQFGLCEENQCVACPTEKGLLGWSKTCMAKKVSSC 362

Query: 364 DPKSFHYYKIEGVDHFLAKYNRGEGPMGQRECEKKCSLDCKCLGYFYQTKGSLCWVANEL 423
           DPKSFHYYK+EGVDHFL K+N+GEG + Q++CEKKC+LDCKCLGYFYQTKGSLCWVANEL
Sbjct: 363 DPKSFHYYKLEGVDHFLTKFNKGEG-LSQKDCEKKCNLDCKCLGYFYQTKGSLCWVANEL 422

Query: 424 KTLIKVANSTHLGFIKTPNK 438
           KTLIKV NSTHLGFIK PNK
Sbjct: 423 KTLIKVDNSTHLGFIKAPNK 440

BLAST of MC10g_new0157 vs. TAIR 10
Match: AT1G78850.1 (D-mannose binding lectin protein with Apple-like carbohydrate-binding domain )

HSP 1 Score: 432.6 bits (1111), Expect = 3.9e-121
Identity = 215/433 (49.65%), Postives = 288/433 (66.51%), Query Frame = 0

Query: 11  LLSCFFLSL----SQALVPPDQTFKFVNEGDFGDFA-VEYGANYRVLSIAQSPFQLAFYN 70
           L  CF LS+    SQA VP D  F+ VNEG + D++ +EY  + R        F+L FYN
Sbjct: 7   LALCFTLSIFLIGSQAKVPVDDQFRVVNEGGYTDYSPIEYNPDVRGFVPFSDNFRLCFYN 66

Query: 71  TTPNAYTLALRMAILRSESAMRWVWEANRGRPVRDNATFSLGADGNLVLADSDGSVVWQS 130
           TTPNAYTLALR+     ES +RWVWEANRG PV++NAT + G DGNLVLA++DG +VWQ+
Sbjct: 67  TTPNAYTLALRIGNRVQESTLRWVWEANRGSPVKENATLTFGEDGNLVLAEADGRLVWQT 126

Query: 131 NTANKGVVGFKLLPNGNMVLHDSSGKFLWQSFDSPTDTLLVGQSLRLGGAIKLVSRASEK 190
           NTANKG VG K+L NGNMV++DSSGKF+WQSFDSPTDTLLVGQSL+L G  KLVSR S  
Sbjct: 127 NTANKGAVGIKILENGNMVIYDSSGKFVWQSFDSPTDTLLVGQSLKLNGRTKLVSRLSPS 186

Query: 191 LNSNGPYSFVMEKKTLNLYYKSPNSPKPMRYFTSSNRFTIQKGSLSKVTLNADVDPDQGF 250
           +N+NGPYS VME K L LYY +  +PKP+ YF     F  +      +T  A  D D  +
Sbjct: 187 VNTNGPYSLVMEAKKLVLYYTTNKTPKPIAYF--EYEFFTKITQFQSMTFQAVEDSDTTW 246

Query: 251 ATEIKLDYESAGSTESGGTIVARPKYNATLTFLRLGIDGNLRLITYNDKVDWGPSEVSFT 310
              + ++   +GS  +  T ++RPK+NATL+F+RL  DGN+R+ +Y+        +V++T
Sbjct: 247 G--LVMEGVDSGSKFNVSTFLSRPKHNATLSFIRLESDGNIRVWSYSTLATSTAWDVTYT 306

Query: 311 LF-DRDSNWESECQWPERCGQLGLCEENQCVGCPTANGVVGWSKSCAAEKVRSCDPKSFH 370
            F + D++   EC+ PE C   GLC++ QC  CP+  G++GW ++C +  + SCDPK+FH
Sbjct: 307 AFTNADTDGNDECRIPEHCLGFGLCKKGQCNACPSDKGLLGWDETCKSPSLASCDPKTFH 366

Query: 371 YYKIEGVDHFLAKYNRGEGPMGQRECEKKCSLDCKCLGYFYQTKGSLCWVANELKTLIKV 430
           Y+KIEG D F+ KYN G     +  C  KC+ DCKCLG+FY  K S CW+  ELKTL + 
Sbjct: 367 YFKIEGADSFMTKYNGGSSTT-ESACGDKCTRDCKCLGFFYNRKSSRCWLGYELKTLTRT 426

Query: 431 ANSTHLGFIKTPN 438
            +S+ + ++K PN
Sbjct: 427 GDSSLVAYVKAPN 434

BLAST of MC10g_new0157 vs. TAIR 10
Match: AT1G78860.1 (D-mannose binding lectin protein with Apple-like carbohydrate-binding domain )

HSP 1 Score: 427.6 bits (1098), Expect = 1.2e-119
Identity = 212/424 (50.00%), Postives = 283/424 (66.75%), Query Frame = 0

Query: 16  FLSLSQALVPPDQTFKFVNEGDFGDFA-VEYGANYRVLSIAQSPFQLAFYNTTPNAYTLA 75
           FL  +QA VP D  F+ VNEG + D++ +EY  + R        F+L FYNTT NAYTLA
Sbjct: 16  FLVGAQAKVPVDDQFRVVNEGGYTDYSPIEYNPDVRGFVPFSDNFRLCFYNTTQNAYTLA 75

Query: 76  LRMAILRSESAMRWVWEANRGRPVRDNATFSLGADGNLVLADSDGSVVWQSNTANKGVVG 135
           LR+     ES +RWVWEANRG PV++NAT + G DGNLVLA++DG VVWQ+NTANKGVVG
Sbjct: 76  LRIGNRAQESTLRWVWEANRGSPVKENATLTFGEDGNLVLAEADGRVVWQTNTANKGVVG 135

Query: 136 FKLLPNGNMVLHDSSGKFLWQSFDSPTDTLLVGQSLRLGGAIKLVSRASEKLNSNGPYSF 195
            K+L NGNMV++DS+GKF+WQSFDSPTDTLLVGQSL+L G  KLVSR S  +N+NGPYS 
Sbjct: 136 IKILENGNMVIYDSNGKFVWQSFDSPTDTLLVGQSLKLNGQNKLVSRLSPSVNANGPYSL 195

Query: 196 VMEKKTLNLYYKSPNSPKPMRYFTSSNRFTIQKGSLSKVTLNADVDPDQGFATEIKLDYE 255
           VME K L LYY +  +PKP+ Y+     F  +   L  +T  A  D D  +   + ++  
Sbjct: 196 VMEAKKLVLYYTTNKTPKPIGYY--EYEFFTKIAQLQSMTFQAVEDADTTWG--LHMEGV 255

Query: 256 SAGSTESGGTIVARPKYNATLTFLRLGIDGNLRLITYNDKVDWGPSEVSFTLFDRD-SNW 315
            +GS  +  T ++RPK+NATL+FLRL  DGN+R+ +Y+        +V++T F  D ++ 
Sbjct: 256 DSGSQFNVSTFLSRPKHNATLSFLRLESDGNIRVWSYSTLATSTAWDVTYTAFTNDNTDG 315

Query: 316 ESECQWPERCGQLGLCEENQCVGCPTANGVVGWSKSCAAEKVRSCDPKSFHYYKIEGVDH 375
             EC+ PE C   GLC++ QC  CP+  G++GW ++C    + SCDPK+FHY+KIEG D 
Sbjct: 316 NDECRIPEHCLGFGLCKKGQCNACPSDIGLLGWDETCKIPSLASCDPKTFHYFKIEGADS 375

Query: 376 FLAKYNRGEGPMGQRECEKKCSLDCKCLGYFYQTKGSLCWVANELKTLIKVANSTHLGFI 435
           F+ KYN G     +  C  KC+ DCKCLG+FY  K S CW+  ELKTL K  +++ + ++
Sbjct: 376 FMTKYN-GGSTTTESACGDKCTRDCKCLGFFYNRKSSRCWLGYELKTLTKTGDTSLVAYV 434

Query: 436 KTPN 438
           K PN
Sbjct: 436 KAPN 434

BLAST of MC10g_new0157 vs. TAIR 10
Match: AT1G16905.1 (Curculin-like (mannose-binding) lectin family protein )

HSP 1 Score: 396.4 bits (1017), Expect = 3.1e-110
Identity = 207/431 (48.03%), Postives = 281/431 (65.20%), Query Frame = 0

Query: 10  LLLSCFFL-SLSQALVPPDQTFKFVNEGDFGDFAVEYGANYRVLSIAQSPFQLAFYNTTP 69
           +LLS F L SL +  VPP + F+F+N GDFG+  VEYGA+YR L + ++ F+L F+NTTP
Sbjct: 9   ILLSLFLLISLVRPQVPPMEQFRFLNNGDFGESTVEYGASYRDLGVIRNQFRLCFFNTTP 68

Query: 70  NAYTLALRMAILRSESAMRWVWEANRGRPVRDNATFSLGADGNLVLADSDGSVVWQSNTA 129
           NA+TLA+ M    S+S +RWVW+AN  +PV++ A+ S G +GNLVLA  DG VVWQ+ T 
Sbjct: 69  NAFTLAIGMGTGSSDSIIRWVWQANPQKPVQEEASLSFGPEGNLVLAQPDGRVVWQTMTE 128

Query: 130 NKGVVGFKLLPNGNMVLHDSSGKFLWQSFDSPTDTLLVGQSLRLGGA-IKLVSRASEKLN 189
           NKGV+G  +  NGN+VL D  G  +WQSF+ PTDTLLVGQSL L G+  KLVSR      
Sbjct: 129 NKGVIGLTMNENGNLVLFDDGGWPVWQSFEFPTDTLLVGQSLTLDGSKNKLVSR------ 188

Query: 190 SNGPYSFVMEKKTLNLYYKSPNS-PKPMRYFTSSNRFTIQKGSLSKVTLNADVDPDQGFA 249
           +NG YS ++E   L L    P S  K + Y     RF      +   TL +    DQG  
Sbjct: 189 NNGSYSLILEPDRLVLNRLIPRSNNKSLVYHIIEGRF------IPSATLYS--AKDQGTT 248

Query: 250 TEIKLDYESAGSTESGGTIVARPKYNATLTFLRLGIDGNLRLITYNDKVDWGPSEVSFTL 309
           T++ L              +ARP++NA+ +FLRL  DGNLR+ +++ KV +   EV+F L
Sbjct: 249 TQLGLATPGLRPEFPYKHFLARPRFNASQSFLRLDADGNLRIYSFDSKVTFLAWEVTFEL 308

Query: 310 FDRDSNWESECQWPERCGQLGLCEENQCVGCPTANGVVGWSKSCAAEKVRSCDPKSFHYY 369
           F+ D+N  +EC  P +CG  G+CE+NQCV CP   G++GWSK+C  +KV+SCDPKSFHYY
Sbjct: 309 FNHDNN--NECWLPSKCGAFGICEDNQCVACPLGVGLMGWSKACKPKKVKSCDPKSFHYY 368

Query: 370 KIEGVDHFLAKYNRGEGPMGQRECEKKCSLDCKCLGYFYQTKGSLCWVANELKTLIKVAN 429
           ++ GV+HF+ KYN G   +G+ +C   CS DCKCLGYF+      CW++ EL TL+KV++
Sbjct: 369 RLGGVEHFMTKYNVGLA-LGESKCRGLCSGDCKCLGYFFDKSSFKCWISYELGTLVKVSD 422

Query: 430 STHLGFIKTPN 438
           S  + +IKTPN
Sbjct: 429 SRKVAYIKTPN 422

BLAST of MC10g_new0157 vs. TAIR 10
Match: AT1G78830.1 (Curculin-like (mannose-binding) lectin family protein )

HSP 1 Score: 376.7 bits (966), Expect = 2.5e-104
Identity = 203/453 (44.81%), Postives = 273/453 (60.26%), Query Frame = 0

Query: 6   LLTPLLLSCFFLSLSQALVPPDQTFKFVNEGDFGDFAVEYGANYRVL-----SIAQSPFQ 65
           +L  L L+   +S+  A VPP++ F+ VNEG+FG++  EY A+YR +     S   SPFQ
Sbjct: 6   ILVTLALAIATVSVVIAQVPPEKQFRVVNEGEFGEYITEYDASYRFIESSNQSFFTSPFQ 65

Query: 66  LAFYNTTPNAYTLALRMAILRSESAMRWVWEANRGRPVRDNATFSLGADGNLVLADSDGS 125
           L FYNTTP+AY LALR+ + R ES MRW+W+ANR  PV +NAT SLG +GNLVLA++DG 
Sbjct: 66  LLFYNTTPSAYILALRVGLRRDESTMRWIWDANRNNPVGENATLSLGRNGNLVLAEADGR 125

Query: 126 VVWQSNTANKGVVGFKLLPNGNMVLHDSSGKFLWQSFDSPTDTLLVGQSLRLGGAIKLVS 185
           V WQ+NTANKGV GF++LPNGN+VLHD +GKF+WQSFD PTDTLL GQSL++ G  KLVS
Sbjct: 126 VKWQTNTANKGVTGFQILPNGNIVLHDKNGKFVWQSFDHPTDTLLTGQSLKVNGVNKLVS 185

Query: 186 RASEKLNSNGPYSFVMEKKTLNLYYKSPNSPK-----PMRYFTSSNRFTIQK-------G 245
           R S+   S+GPYS V++KK L +Y     +P      P   F  +  F + +        
Sbjct: 186 RTSDSNGSDGPYSMVLDKKGLTMYVNKTGTPLVYGGWPDHDFRGTVTFAVTREFDNLTEP 245

Query: 246 SLSKVTLNADVDPDQGFATEIK-LDYESAGSTESGGTI-VARPKYNATLTFLRLGIDGNL 305
           S  ++ L     P        + L     GS   GGT+ + +  YN T+++LRLG DG+L
Sbjct: 246 SAYELLLEPAPQPATNPGNNRRLLQVRPIGS--GGGTLNLNKINYNGTISYLRLGSDGSL 305

Query: 306 RLITYNDKVDWGPSEVSFTLFDRDSNWESECQWPERCGQLGLCEENQCVGCPTANGVVGW 365
           +  +Y     +   E SF+ F   + +  +C  P  CG  G C+   C  CPT  G++GW
Sbjct: 306 KAYSYFPAATYLKWEESFSFF--STYFVRQCGLPSFCGDYGYCDRGMCNACPTPKGLLGW 365

Query: 366 SKSCAAEKV----RSCDPKSFHYYKIEGVDHFLAKY-NRGEGPMGQRECEKKCSLDCKCL 425
           S  CA  K          K+ +YYKI GV+HF   Y N G+GP    +C+ KC  DCKCL
Sbjct: 366 SDKCAPPKTTQFCSGVKGKTVNYYKIVGVEHFTGPYVNDGQGPTSVNDCKAKCDRDCKCL 425

Query: 426 GYFYQTKGSLCWVANELKTLIKVANSTHLGFIK 435
           GYFY+ K   C +A  L TLIK AN++ + +IK
Sbjct: 426 GYFYKEKDKKCLLAPLLGTLIKDANTSSVAYIK 454

BLAST of MC10g_new0157 vs. TAIR 10
Match: AT1G78820.1 (D-mannose binding lectin protein with Apple-like carbohydrate-binding domain )

HSP 1 Score: 354.4 bits (908), Expect = 1.3e-97
Identity = 194/455 (42.64%), Postives = 267/455 (58.68%), Query Frame = 0

Query: 6   LLTPLLLSCFFLSLSQALVPPDQTFKFVNEGDFGDFAVEYGANYRVLSIAQS-----PFQ 65
           L+T L +S   +S+  A VPP++ F+ +NE  +  +  EY A+YR L+         PFQ
Sbjct: 8   LITALAIST--VSVVMAQVPPEKQFRVLNEPGYAPYITEYDASYRFLNSPNQNFFTIPFQ 67

Query: 66  LAFYNTTPNAYTLALRMAILRSESAMRWVWEANRGRPVRDNATFSLGADGNLVLADSDGS 125
           L FYNTTP+AY LALR+   R  S  RW+W+ANR  PV DN+T S G +GNLVLA+ +G 
Sbjct: 68  LMFYNTTPSAYVLALRVGTRRDMSFTRWIWDANRNNPVGDNSTLSFGRNGNLVLAELNGQ 127

Query: 126 VVWQSNTANKGVVGFKLLPNGNMVLHDSSGKFLWQSFDSPTDTLLVGQSLRLGGAIKLVS 185
           V WQ+NTANKGV GF++LPNGNMVLHD  GKF+WQSFD PTDTLLVGQSL++ G  KLVS
Sbjct: 128 VKWQTNTANKGVTGFQILPNGNMVLHDKHGKFVWQSFDHPTDTLLVGQSLKVNGVNKLVS 187

Query: 186 RASEKLNSNGPYSFVMEKKTLNLYYKSPNSPKPMRYFTSSNRFTIQKGSLSKVTLNADVD 245
           R S+   S+GPYS V++ K L +Y     +P     +T  +     +G+++        +
Sbjct: 188 RTSDMNGSDGPYSMVLDNKGLTMYVNKTGTPLVYGGWTDHD----FRGTVTFAVTREFDN 247

Query: 246 PDQGFATEIKLDYESAGST---------------ESGGTI-VARPKYNATLTFLRLGIDG 305
             +  A E+ L+     +T                 GGT+ + +  YN T+++LRLG DG
Sbjct: 248 LTEPSAYELLLEPAPQPATNPGNNRRLLQVRPIGSGGGTLNLNKINYNGTISYLRLGSDG 307

Query: 306 NLRLITYNDKVDWGPSEVSFTLFDRDSNWESECQWPERCGQLGLCEENQCVGCPTANGVV 365
           +L+  +Y     +   E +F  F   + +  +C  P  CG  G C+   CVGCPT  G++
Sbjct: 308 SLKAFSYFPAATYLEWEETFAFF--SNYFVRQCGLPTFCGDYGYCDRGMCVGCPTPKGLL 367

Query: 366 GWSKSCAAEK-VRSCD---PKSFHYYKIEGVDHFLAKY-NRGEGPMGQRECEKKCSLDCK 425
            WS  CA  K  + C     K+ +YYKI GV+HF   Y N G+GP    +C+ KC  DCK
Sbjct: 368 AWSDKCAPPKTTQFCSGGKGKAVNYYKIVGVEHFTGPYVNDGQGPTSVNDCKAKCDRDCK 427

Query: 426 CLGYFYQTKGSLCWVANELKTLIKVANSTHLGFIK 435
           CLGYFY+ K   C +A  L TLIK AN++ + +IK
Sbjct: 428 CLGYFYKEKDKKCLLAPLLGTLIKDANTSSVAYIK 454

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZVA45.4e-12049.65EP1-like glycoprotein 3 OS=Arabidopsis thaliana OX=3702 GN=At1g78850 PE=1 SV=1[more]
Q9ZVA51.8e-11850.00EP1-like glycoprotein 4 OS=Arabidopsis thaliana OX=3702 GN=At1g78860 PE=3 SV=1[more]
Q396884.8e-11656.69Epidermis-specific secreted glycoprotein EP1 OS=Daucus carota OX=4039 GN=EP1 PE=... [more]
Q9ZVA23.5e-10344.81EP1-like glycoprotein 2 OS=Arabidopsis thaliana OX=3702 GN=At1g78830 PE=1 SV=1[more]
Q9ZVA11.9e-9642.64EP1-like glycoprotein 1 OS=Arabidopsis thaliana OX=3702 GN=At1g78820 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_038898869.11.88e-28084.17epidermis-specific secreted glycoprotein EP1-like isoform X1 [Benincasa hispida][more]
XP_038880567.15.38e-28084.17epidermis-specific secreted glycoprotein EP1-like [Benincasa hispida][more]
XP_038898943.15.14e-27883.49epidermis-specific secreted glycoprotein EP1-like isoform X2 [Benincasa hispida][more]
XP_038899017.11.08e-27783.75epidermis-specific secreted glycoprotein EP1-like isoform X3 [Benincasa hispida][more]
XP_023551867.11.72e-27682.80epidermis-specific secreted glycoprotein EP1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1ETQ83.11e-27481.65epidermis-specific secreted glycoprotein EP1-like OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1JA713.85e-26879.82epidermis-specific secreted glycoprotein EP1-like OS=Cucurbita maxima OX=3661 GN... [more]
A0A0A0L0B91.87e-26480.68Bulb-type lectin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G0... [more]
A0A5A7V4R15.36e-26480.45Epidermis-specific secreted glycoprotein EP1-like OS=Cucumis melo var. makuwa OX... [more]
A0A5D3CWN66.25e-26380.00Epidermis-specific secreted glycoprotein EP1-like OS=Cucumis melo var. makuwa OX... [more]
Match NameE-valueIdentityDescription
AT1G78850.13.9e-12149.65D-mannose binding lectin protein with Apple-like carbohydrate-binding domain [more]
AT1G78860.11.2e-11950.00D-mannose binding lectin protein with Apple-like carbohydrate-binding domain [more]
AT1G16905.13.1e-11048.03Curculin-like (mannose-binding) lectin family protein [more]
AT1G78830.12.5e-10444.81Curculin-like (mannose-binding) lectin family protein [more]
AT1G78820.11.3e-9742.64D-mannose binding lectin protein with Apple-like carbohydrate-binding domain [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001480Bulb-type lectin domainSMARTSM00108blect_4coord: 43..160
e-value: 6.0E-37
score: 138.7
IPR001480Bulb-type lectin domainPFAMPF01453B_lectincoord: 89..172
e-value: 2.7E-19
score: 69.6
IPR001480Bulb-type lectin domainPROSITEPS50927BULB_LECTINcoord: 8..158
score: 15.279498
IPR001480Bulb-type lectin domainCDDcd00028B_lectincoord: 43..160
e-value: 2.13261E-35
score: 125.117
IPR036426Bulb-type lectin domain superfamilyGENE3D2.90.10.10coord: 15..158
e-value: 1.4E-16
score: 62.6
IPR036426Bulb-type lectin domain superfamilySUPERFAMILY51110alpha-D-mannose-specific plant lectinscoord: 68..207
IPR035446S-locus-specific glycoprotein/EP1PIRSFPIRSF002686SLGcoord: 1..438
e-value: 4.9E-148
score: 491.3
NoneNo IPR availablePANTHERPTHR32444:SF64SECRETED GLYCOPROTEIN EP1, PUTATIVE-RELATEDcoord: 14..438
NoneNo IPR availablePANTHERPTHR32444FAMILY NOT NAMEDcoord: 14..438
NoneNo IPR availableCDDcd01098PAN_AP_plantcoord: 356..436
e-value: 1.69853E-11
score: 58.2166

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC10g_new0157.1MC10g_new0157.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0110165 cellular anatomical entity