MC10g1386 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC10g1386
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionF-box/kelch-repeat protein SKIP25
LocationMC10: 17291344 .. 17292492 (-)
RNA-Seq ExpressionMC10g1386
SyntenyMC10g1386
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTCACCGCTGCCACTTCCCCCGCCGTCCCCTTTGACTCCCCCTCCAAGCGCCGGAAACTGGCTCTCCACCACCGCCCCTCTCCGCCCCTCCTCCCGGATCTTCCCGATGACGTCGCCCACTTCTGCCTCTCCCATCTCCCACCTTCCCTCCTCTTCTCCGTCTGCCGTTCATGGCGGCGCCTCATCTATTCCCCTTCGTTTCCCCCTTTTCTCTCTCTATACGCCGTCGTTTCCTCCTCCTCTCTCTCTCCTCCATCAAACCCCTCTCCCAATTCCCTCCAATTCTTCAATTTCGATCCAATCTCTGCTAAATGGAACCCCCTCCCTCTTCCCCCAAATCGCCCGCCGTTGAATCTCGCGATCCGCCACCCCTCCTTCATTTCCCGGAATCTCCCCGTCCAGTCCATCGCCGTCGCCGGCAAGCTCGTCCTCCTCGCCGCCACCGCCGACAACTTCGCCCCAGCCCTCCCTCGCCCTCTCATCTTCTCCCCTCTCTCCGGCTCCTGGGAGTTCGGCCCACCCCTCGACAGCCCCCGCCGGTGGTGCGCCGCCGGAGCCCTCCGCGGATCCGTCTACGTCGCCAGCGGAATCGGGTCATTCTTCTCCTCCGACGTCGCTCGCTCCGTCGAGAGATGGGAGTTGAAACCCGGCGGCGGAGGTGATCGGTCGGTTTGGGAGAAGGTGAGTGGGTTGAAGGATGGGAGATTCAGCAGAGACGCGATTGATGCTGTGGGGTGGAGGGGGAAGCTGTGTATGGTGAATGTGAAAGGGGATGCGGCGAAGGAAGGGGCGGTGTACGATCTGGAGAAGGATGAGTGGGAGGAGATGCCGGAGGGGATGATCGGAGGGTGGAGAGGGCCGGTGGCGGCCATGGATGAGGAGGTCATGTATGTGGTGGAGGAGGGGAGCGGTAGTTTTCGAAGGTACGATCCGGAGAGGGATTTGTGGGAGGAGATGATGGAGTCGAATAGGCTCAAAGGGGCGGTGCAGATGGCGGCCGGCGGCGGGAGAGCGTGCGTCGTCTGCGGCGGCGGAAGTAGAGGGGAAATCGCGGTGGTGGACGTGGCCGCGTCGCCGCCGAGGCTCTGGTTCGAGCCGGCCCCGGCCGGGATGGAGGTCGTCGCCGTCCACGTGCTGCCGAGAATG

mRNA sequence

ATGGTCACCGCTGCCACTTCCCCCGCCGTCCCCTTTGACTCCCCCTCCAAGCGCCGGAAACTGGCTCTCCACCACCGCCCCTCTCCGCCCCTCCTCCCGGATCTTCCCGATGACGTCGCCCACTTCTGCCTCTCCCATCTCCCACCTTCCCTCCTCTTCTCCGTCTGCCGTTCATGGCGGCGCCTCATCTATTCCCCTTCGTTTCCCCCTTTTCTCTCTCTATACGCCGTCGTTTCCTCCTCCTCTCTCTCTCCTCCATCAAACCCCTCTCCCAATTCCCTCCAATTCTTCAATTTCGATCCAATCTCTGCTAAATGGAACCCCCTCCCTCTTCCCCCAAATCGCCCGCCGTTGAATCTCGCGATCCGCCACCCCTCCTTCATTTCCCGGAATCTCCCCGTCCAGTCCATCGCCGTCGCCGGCAAGCTCGTCCTCCTCGCCGCCACCGCCGACAACTTCGCCCCAGCCCTCCCTCGCCCTCTCATCTTCTCCCCTCTCTCCGGCTCCTGGGAGTTCGGCCCACCCCTCGACAGCCCCCGCCGGTGGTGCGCCGCCGGAGCCCTCCGCGGATCCGTCTACGTCGCCAGCGGAATCGGGTCATTCTTCTCCTCCGACGTCGCTCGCTCCGTCGAGAGATGGGAGTTGAAACCCGGCGGCGGAGGTGATCGGTCGGTTTGGGAGAAGGTGAGTGGGTTGAAGGATGGGAGATTCAGCAGAGACGCGATTGATGCTGTGGGGTGGAGGGGGAAGCTGTGTATGGTGAATGTGAAAGGGGATGCGGCGAAGGAAGGGGCGGTGTACGATCTGGAGAAGGATGAGTGGGAGGAGATGCCGGAGGGGATGATCGGAGGGTGGAGAGGGCCGGTGGCGGCCATGGATGAGGAGGTCATGTATGTGGTGGAGGAGGGGAGCGGTAGTTTTCGAAGGTACGATCCGGAGAGGGATTTGTGGGAGGAGATGATGGAGTCGAATAGGCTCAAAGGGGCGGTGCAGATGGCGGCCGGCGGCGGGAGAGCGTGCGTCGTCTGCGGCGGCGGAAGTAGAGGGGAAATCGCGGTGGTGGACGTGGCCGCGTCGCCGCCGAGGCTCTGGTTCGAGCCGGCCCCGGCCGGGATGGAGGTCGTCGCCGTCCACGTGCTGCCGAGAATG

Coding sequence (CDS)

ATGGTCACCGCTGCCACTTCCCCCGCCGTCCCCTTTGACTCCCCCTCCAAGCGCCGGAAACTGGCTCTCCACCACCGCCCCTCTCCGCCCCTCCTCCCGGATCTTCCCGATGACGTCGCCCACTTCTGCCTCTCCCATCTCCCACCTTCCCTCCTCTTCTCCGTCTGCCGTTCATGGCGGCGCCTCATCTATTCCCCTTCGTTTCCCCCTTTTCTCTCTCTATACGCCGTCGTTTCCTCCTCCTCTCTCTCTCCTCCATCAAACCCCTCTCCCAATTCCCTCCAATTCTTCAATTTCGATCCAATCTCTGCTAAATGGAACCCCCTCCCTCTTCCCCCAAATCGCCCGCCGTTGAATCTCGCGATCCGCCACCCCTCCTTCATTTCCCGGAATCTCCCCGTCCAGTCCATCGCCGTCGCCGGCAAGCTCGTCCTCCTCGCCGCCACCGCCGACAACTTCGCCCCAGCCCTCCCTCGCCCTCTCATCTTCTCCCCTCTCTCCGGCTCCTGGGAGTTCGGCCCACCCCTCGACAGCCCCCGCCGGTGGTGCGCCGCCGGAGCCCTCCGCGGATCCGTCTACGTCGCCAGCGGAATCGGGTCATTCTTCTCCTCCGACGTCGCTCGCTCCGTCGAGAGATGGGAGTTGAAACCCGGCGGCGGAGGTGATCGGTCGGTTTGGGAGAAGGTGAGTGGGTTGAAGGATGGGAGATTCAGCAGAGACGCGATTGATGCTGTGGGGTGGAGGGGGAAGCTGTGTATGGTGAATGTGAAAGGGGATGCGGCGAAGGAAGGGGCGGTGTACGATCTGGAGAAGGATGAGTGGGAGGAGATGCCGGAGGGGATGATCGGAGGGTGGAGAGGGCCGGTGGCGGCCATGGATGAGGAGGTCATGTATGTGGTGGAGGAGGGGAGCGGTAGTTTTCGAAGGTACGATCCGGAGAGGGATTTGTGGGAGGAGATGATGGAGTCGAATAGGCTCAAAGGGGCGGTGCAGATGGCGGCCGGCGGCGGGAGAGCGTGCGTCGTCTGCGGCGGCGGAAGTAGAGGGGAAATCGCGGTGGTGGACGTGGCCGCGTCGCCGCCGAGGCTCTGGTTCGAGCCGGCCCCGGCCGGGATGGAGGTCGTCGCCGTCCACGTGCTGCCGAGAATG

Protein sequence

MVTAATSPAVPFDSPSKRRKLALHHRPSPPLLPDLPDDVAHFCLSHLPPSLLFSVCRSWRRLIYSPSFPPFLSLYAVVSSSSLSPPSNPSPNSLQFFNFDPISAKWNPLPLPPNRPPLNLAIRHPSFISRNLPVQSIAVAGKLVLLAATADNFAPALPRPLIFSPLSGSWEFGPPLDSPRRWCAAGALRGSVYVASGIGSFFSSDVARSVERWELKPGGGGDRSVWEKVSGLKDGRFSRDAIDAVGWRGKLCMVNVKGDAAKEGAVYDLEKDEWEEMPEGMIGGWRGPVAAMDEEVMYVVEEGSGSFRRYDPERDLWEEMMESNRLKGAVQMAAGGGRACVVCGGGSRGEIAVVDVAASPPRLWFEPAPAGMEVVAVHVLPRM
Homology
BLAST of MC10g1386 vs. ExPASy Swiss-Prot
Match: Q8GX29 (F-box/kelch-repeat protein SKIP25 OS=Arabidopsis thaliana OX=3702 GN=SKIP25 PE=1 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 1.3e-101
Identity = 204/389 (52.44%), Postives = 265/389 (68.12%), Query Frame = 0

Query: 3   TAATSPAVPFDSPSKRRKLALHHRPSPPLLPDLPDDVAHFCLSHL-PPSLLFSVCRSWRR 62
           TAA SP      P+KRR+    +  S  L+  LPD ++  CLS +  PSLL +VC  WRR
Sbjct: 14  TAAESP------PAKRRRTVTGNENS-ALIEGLPDHISEICLSLVHRPSLLSAVCTRWRR 73

Query: 63  LIYSPSFPPFLSLYAV-VSSSSLSPPSNPSPNSLQFFNFDPISAKWNPLPLPPNRPPLN- 122
           L+YSP FP F SLYA+ V S+S +   NP   S++F  F+P+S+KW PLP PP  PPL+ 
Sbjct: 74  LLYSPEFPSFPSLYALFVDSTSDTGRVNP---SVRFMCFNPVSSKWYPLPPPPPDPPLHR 133

Query: 123 LAIRHPSFISRNLPVQSIAVAGKLVLLAATADNFAPALPRPLIFSPLSGSWEFGPPLDSP 182
           +  RHPSFIS NLP+Q ++ AGKL+L+A +    +PA+  PLIF P+S SW  GP + SP
Sbjct: 134 ILYRHPSFISFNLPIQCVSAAGKLILIAGSNQQLSPAISHPLIFDPISSSWSSGPRIGSP 193

Query: 183 RRWCAAGALRGSVYVASGIGSFFSSDVARSVERWEL-KPGGGGDRSVWEKVSGLKDGRFS 242
           RRWCA GA  G++Y+ASGI S FSS VA+SVE+ +L +      R  WEK+  ++D RFS
Sbjct: 194 RRWCATGACDGAIYIASGISSQFSSTVAKSVEKLDLTEQNRNNHRFNWEKLRDMRDLRFS 253

Query: 243 RDAIDAVGWRGKLCMVNVKGDAAKEGAVYDLEKDEWEEMPEGMIGGWRGPVAAMDEEVMY 302
           R+AIDAVG+R KL MVNVKGDA KEGA+YD+ KD+WE MPE M+ GWRGPVAAM+EE++Y
Sbjct: 254 REAIDAVGYRRKLLMVNVKGDAVKEGAIYDVVKDDWEPMPEEMLVGWRGPVAAMEEEILY 313

Query: 303 VVEEGSGSFRRYDPERDLWEEMM----ESNRLKGAVQMAAGGGRACVVCGGGSRGEIAVV 362
            V+E  G+ R+YD E+  W E++        LKGA Q+ A  G+ CVV G    G+I VV
Sbjct: 314 SVDERRGTVRKYDDEKREWREVVVVEGGEEMLKGATQVTADSGKLCVVTGD---GKIVVV 373

Query: 363 DVAASPPRLWFEPAPAGMEVVAVHVLPRM 384
           DVAA P ++W    P G+E V+VHVLPRM
Sbjct: 374 DVAAEPAKIWNVEIPDGLEPVSVHVLPRM 389

BLAST of MC10g1386 vs. ExPASy Swiss-Prot
Match: Q8LAW2 (F-box protein AFR OS=Arabidopsis thaliana OX=3702 GN=AFR PE=1 SV=2)

HSP 1 Score: 81.3 bits (199), Expect = 2.7e-14
Identity = 92/380 (24.21%), Postives = 146/380 (38.42%), Query Frame = 0

Query: 28  SPPLLPDLPDDVAHFCLSHLP---PSLLFSVCRSWRRLIYSPSFPPFLSLYAVVSSSSLS 87
           S PL+  LP+D+A  CL  LP    +L  SV  SW + I +P F     L++  S S  S
Sbjct: 25  SQPLISGLPNDIAELCLLRLPYPYHALYRSVSSSWNKTITNPRF-----LFSKQSLSISS 84

Query: 88  P-----PSNPSPNSLQFFNFDPISAKWNPLPLPPNRPPLNLAIRHPSFISRNLPVQSIAV 147
           P       N S   +Q+ + D  S +W  LP  PN       I  P  +S      S+  
Sbjct: 85  PYLFVFAFNKSTARIQWQSLDLASGRWFVLPPMPNS---FTKISSPHALS----CASMPR 144

Query: 148 AGKLVLLAATADNFAPALPRPLIFSPLSGSWEFGPPLDSPRRWCAAGALRGSVYVASG-I 207
            GKL +L     N +      ++++ L+  W    P+ SPR +  +G + G +    G +
Sbjct: 145 QGKLFVLGGGDVNRS-----AVVYTALTNRWSCISPMMSPRTYFVSGNVNGKIMAVGGSV 204

Query: 208 GSFFSSDVARSVERWELKPGGGGDRSVWEKVSGL-------KDGRFSRDAIDAVGWRGKL 267
           G   + +    VE ++       D   W  V  L             ++     GW    
Sbjct: 205 GG--NGEATTEVESYD------PDNDTWTVVKKLPMVLAKYDSAVIGKEMCVTEGWAWPF 264

Query: 268 CMVNVKGDAAKEGAVYDLEKDEWEEMPEGMIGGWRGPVAAMDEEVMYVVEEGSGSFRRYD 327
               +       G VYD ++  W EM  GM  GW G    + + +  + E G    + Y 
Sbjct: 265 MFPPM-------GQVYDSDEGTWREMSGGMKEGWTGVSVVIRDRLFVISEHGDFPMKVYC 324

Query: 328 PERDLWE----EMMESNRLKGAVQMAAGGGRACVVCGG----------GSRGEIAVVDVA 378
            + D W     E ++  +++    +     R  VV  G          G  G+ +V    
Sbjct: 325 SDDDTWRYVSGEKLQGEKMRRPFAVTGADDRVFVVASGINVAEGRVSEGQNGDFSVEWRM 372

BLAST of MC10g1386 vs. ExPASy Swiss-Prot
Match: Q93W93 (F-box/kelch-repeat protein At1g55270 OS=Arabidopsis thaliana OX=3702 GN=At1g55270 PE=2 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 5.6e-12
Identity = 80/355 (22.54%), Postives = 133/355 (37.46%), Query Frame = 0

Query: 26  RPSPPLLPDLPDDVAHFCLSHLPPS---LLFSVCRSWRRLIYSPSFPPFLSLYAVVSSSS 85
           R  PPLLP LPDD+A  CL  +P +    L  VC+ W RL     F     L   +S   
Sbjct: 73  RIQPPLLPGLPDDLAVACLIRVPRAEHRKLRLVCKRWYRLASGNFFYSQRKLLG-MSEEW 132

Query: 86  LSPPSNPSPNSLQFFNFDPISAKWNPLPLPPNRPPLNLAIRHPSFISRNLPVQSIAVAGK 145
           +          + +  FDPIS  W PLP  P               S  +      ++G 
Sbjct: 133 VYVFKRDRDGKISWNTFDPISQLWQPLPPVPRE------------YSEAVGFGCAVLSGC 192

Query: 146 LVLLAATADNFAPALPRPLIFSPLSGSWEFGPPLDSPRRWCAAGALRGSVYVASGIGSFF 205
            + L    D    ++ R + ++  +  W   P +   R +     +   +YVA G     
Sbjct: 193 HLYLFGGKDPLRGSMRRVIFYNARTNKWHRAPDMLRKRHFFGCCVINNCLYVAGGECEGI 252

Query: 206 SSDVARSVERWELKPGGGGDRSVWEKVSGLKDGRFSRDAIDAVGWRGKLCMVNVKGDAAK 265
              + RS E ++       +++ W   S + D   +   +  V +  K  +  +      
Sbjct: 253 QRTL-RSAEVYD------PNKNRW---SFIADMSTAMVPLIGVVYDKKWFLKGLGSHQLV 312

Query: 266 EGAVYDLEKDEWEEMPEGMIGGWRGPVAAMDEEVMYVVEEGSGSFRRYDPERDLWEEMME 325
               YD E + W  + +GM+ GWR P  +++  +  +        R +D   D W + M+
Sbjct: 313 MSEAYDPEVNSWSPVSDGMVAGWRNPCTSLNGRLYGLDCRDGCKLRVFDESTDSWNKFMD 372

Query: 326 SNRLKG------AVQMAAGGGRACVVCGGGSRGEIAVVDVAASPPRLWFEPAPAG 372
           S    G      A  +     + C++    S   + V +   + PRLW   A  G
Sbjct: 373 SKAHLGNSKSLEAAALVPLHNKLCIIRNNMSMSLVDVSNPDKNNPRLWENIAVKG 404

BLAST of MC10g1386 vs. ExPASy Swiss-Prot
Match: Q9C6Z0 (F-box/kelch-repeat protein At1g30090 OS=Arabidopsis thaliana OX=3702 GN=At1g30090 PE=2 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 6.4e-08
Identity = 76/303 (25.08%), Postives = 116/303 (38.28%), Query Frame = 0

Query: 30  PLLPDLPDDVAHFCLSHLPPSLLF---SVCRSWRRLIYSPSFPPFLSLYAVVSSSSLSPP 89
           PL+P LPDDVA  CL  +P        SVC+ W  L     F    + +A         P
Sbjct: 52  PLIPGLPDDVALNCLLRVPVQSHVSSKSVCKRWHLL-----FGTKETFFAKRKEFGFKDP 111

Query: 90  ------SNPSPNSLQFFNFDPISAKWNPLPLPPNRPPLNLAIRHPSFISRNLPVQ-SIAV 149
                  +     +Q+   D  +  W+ +P  P R      +    F S ++P + ++ V
Sbjct: 112 WLFVVGFSRCTGKIQWKVLDLRNLTWHEIPAMPCRD----KVCPHGFRSVSMPREGTMFV 171

Query: 150 AGKLVLLAATADNFAPALPRPLIFSPLSGSWEFGPPLDSPRRWCAAGALRGSVYVASG-I 209
            G +V     +D+  P L   L +  +   W     + + R + A+G + G +Y A G  
Sbjct: 172 CGGMV-----SDSDCP-LDLVLKYDMVKNHWTVTNKMITARSFFASGVIDGMIYAAGGNA 231

Query: 210 GSFFSSDVARSVERWELKPGGGGDRSVWEKVSGLKDGRFSRDAIDAVGWRGKLCMVN--- 269
              +  D A       L P  G  R V   V+ +        + D     GKL +     
Sbjct: 232 ADLYELDCAE-----VLNPLDGNWRPVSNMVAHMA-------SYDTAVLNGKLLVTEGWL 291

Query: 270 VKGDAAKEGAVYDLEKDEWEEMPEGMIGGWRGPVAAMDEEVMYVVEEGSGSFRRYDPERD 319
                +  G VYD   D+WE M  G+  GW G    + + +  V E      + YDP  D
Sbjct: 292 WPFFVSPRGQVYDPRTDQWETMSMGLREGWTGTSVVIYDRLFIVSELERMKMKVYDPVTD 327

BLAST of MC10g1386 vs. ExPASy Swiss-Prot
Match: Q9LM55 (F-box/kelch-repeat protein At1g22040 OS=Arabidopsis thaliana OX=3702 GN=At1g22040 PE=2 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 1.5e-04
Identity = 92/379 (24.27%), Postives = 139/379 (36.68%), Query Frame = 0

Query: 5   ATSPAVPFDSPS-KRRKLALHHRPSP--PLLPDLPDDVAHFCLSHLPP---SLLFSVCRS 64
           ATS  V   S S KRRK++  +       L+P LPD+++   L+ LP    S +  V R 
Sbjct: 14  ATSQDVECSSESRKRRKISSENDEEECCRLIPSLPDELSIQILARLPRICYSSVRLVSRR 73

Query: 65  WRRLIYSPSFPPFLSLYAVVSSSSLSPPSNPSPNSLQFFNFDPISAKWNPLPLPP----- 124
           WR  + S S    L      +   L   +    + L ++  DP+S KW  LP  P     
Sbjct: 74  WRSAV-STSEVYSLRKELGRTEEWLYVLTKGHEDKLLWYALDPVSTKWQRLPPMPVVVYE 133

Query: 125 -------------NRPPLNLAIRHPSFISR-----NLPVQSIAV----AGKLVLLAATAD 184
                          P  N+     SF+ R      +P    A+     G  V+   +  
Sbjct: 134 EESRKSLSGLWNMITPSFNVGAIVRSFLGRRDSSEQMPFCGCAIGAVDGGLYVIGGLSRS 193

Query: 185 NFAPALPRPLIFSPLSGSWEFGPPLDSPRRWCAAGALRGSVYVASGI-------GSFFSS 244
                + R   F P+  SW     + + R +   G L   +YV  G+           S+
Sbjct: 194 KTVSCVWR---FDPILNSWSEVSSMLASRAYSKTGVLNKKLYVVGGVDRGRGGLSPLQSA 253

Query: 245 DV-ARSVERWELKPGGGGDRSVWEKVSGLKDGRFSRDAIDAVG-----WRGKLCM----- 304
           +V   S + W   P        + K   L +  F  D +  +      + G+LC+     
Sbjct: 254 EVYDPSTDAWSEVPS-----MPFSKAQVLPNA-FLADLLKPIATGMTCYNGRLCVPQSLY 313

Query: 305 -----VNVKGDAAKEGAVYDLEKDEWEEMPEGMIGGWRGPVAAMDEEV-----MYVVEEG 319
                V+V G+      VYD E + W EMP GM  GW    A     V     +Y  +  
Sbjct: 314 SWPFFVDVGGE------VYDPETNLWVEMPSGMGEGWPARQAGTKLSVVVDGELYAFDPS 373

BLAST of MC10g1386 vs. NCBI nr
Match: XP_008448576.1 (PREDICTED: F-box/kelch-repeat protein SKIP25 [Cucumis melo] >KAA0052979.1 F-box/kelch-repeat protein SKIP25 [Cucumis melo var. makuwa] >TYK11435.1 F-box/kelch-repeat protein SKIP25 [Cucumis melo var. makuwa])

HSP 1 Score: 578 bits (1489), Expect = 6.46e-205
Identity = 292/383 (76.24%), Postives = 316/383 (82.51%), Query Frame = 0

Query: 1   MVTAATSPAVPFDSPSKRRKLALHHRPSPPLLPDLPDDVAHFCLSHLPPSLLFSVCRSWR 60
           MVT AT+ + P    SKR KLA H  PS PLLP LPD VA FCLSH+PPSLLFSV RSWR
Sbjct: 1   MVTTATADS-PTPPSSKRNKLAFHQHPSSPLLPGLPDHVAQFCLSHVPPSLLFSVSRSWR 60

Query: 61  RLIYSPSFPPFLSLYAVVSSSSLSPPSNPSPNSLQFFNFDPISAKWNPLPLPPNRPPLNL 120
           RL+YSPSFPPF SLYA++SSSS         NSL FFNFDPIS+KW+PLP PPN P  +L
Sbjct: 61  RLLYSPSFPPFSSLYALLSSSS---------NSLDFFNFDPISSKWSPLPPPPNSPSSHL 120

Query: 121 AIRHPSFISRNLPVQSIAVAGKLVLLAATADNFAPALPRPLIFSPLSGSWEFGPPLDSPR 180
            I HPSF+SR+LPVQS++V+G L+LLAAT  N  PALPRPL+FSP S SW   PPL +PR
Sbjct: 121 LIHHPSFLSRHLPVQSLSVSGHLLLLAATTHNLLPALPRPLLFSPFSNSWRLAPPLPTPR 180

Query: 181 RWCAAGALRGSVYVASGIGSFFSSDVARSVERWELKPGGGGDRSVWEKVSGLKDGRFSRD 240
           RWCAAGALRGSVYVASGIGSFFS+DVARSVERW+LK  G GD   WEKVSGLKDGRFSRD
Sbjct: 181 RWCAAGALRGSVYVASGIGSFFSTDVARSVERWDLKANGAGD---WEKVSGLKDGRFSRD 240

Query: 241 AIDAVGWRGKLCMVNVKGDAAKEGAVYDLEKDEWEEMPEGMIGGWRGPVAAMDEEVMYVV 300
           AIDAVGW+GKLCMVNVKG A KEG VYDLEKDEWE+MPEGMIGGWRGP AAMDEE MYVV
Sbjct: 241 AIDAVGWKGKLCMVNVKGHALKEGLVYDLEKDEWEDMPEGMIGGWRGPAAAMDEEDMYVV 300

Query: 301 EEGSGSFRRYDPERDLWEEMMESNRLKGAVQMAAGGGRACVVCGGGSRGEIAVVDVAASP 360
           +E SGS RRYDPERD WE++MESNRLKGAVQMAAGGGR CVVCGG SRGEI VVDV ASP
Sbjct: 301 DEVSGSLRRYDPERDFWEDVMESNRLKGAVQMAAGGGRVCVVCGG-SRGEIVVVDVVASP 360

Query: 361 PRLWFEPAPAGMEVVAVHVLPRM 383
            RLW EP P G EVVAVHVLPRM
Sbjct: 361 ARLWVEPTPPGTEVVAVHVLPRM 369

BLAST of MC10g1386 vs. NCBI nr
Match: XP_022965312.1 (F-box/kelch-repeat protein SKIP25-like [Cucurbita maxima])

HSP 1 Score: 571 bits (1472), Expect = 2.16e-202
Identity = 294/386 (76.17%), Postives = 316/386 (81.87%), Query Frame = 0

Query: 1   MVTAATSPAVPFDSPSKRRKLALH---HRPSPPLLPDLPDDVAHFCLSHLPPSLLFSVCR 60
           MVTAAT       +P KR KLALH   HRPSPPLLP LPDDVA  CLSHLPPSLLFSVCR
Sbjct: 1   MVTAAT------PTP-KRSKLALHPHHHRPSPPLLPGLPDDVAQLCLSHLPPSLLFSVCR 60

Query: 61  SWRRLIYSPSFPPFLSLYAVVSSSSLSPPSNPSPNSLQFFNFDPISAKWNPLPLPPNRPP 120
           SWRRL+YSPSFPPF SLYA++SSS         P+S+ FFNFDPIS+KW  LP  PN P 
Sbjct: 61  SWRRLLYSPSFPPFFSLYALLSSS---------PDSIDFFNFDPISSKWTSLPPHPNPPS 120

Query: 121 LNLAIRHPSFISRNLPVQSIAVAGKLVLLAATADNFAPALPRPLIFSPLSGSWEFGPPLD 180
           L L I HPSF+SR+LPVQS+AV+G L+LLAAT  N  PALPRPL+FSPLS SW F PPL 
Sbjct: 121 LQLLIHHPSFLSRHLPVQSLAVSGHLLLLAATTHNLLPALPRPLLFSPLSNSWRFAPPLP 180

Query: 181 SPRRWCAAGALRGSVYVASGIGSFFSSDVARSVERWELKPGGGGDRSVWEKVSGLKDGRF 240
           +PRRWCAAG+LRGSVYVASGIGSFFS+DVARSVERW+LKP  GG    WEKVSGLKDGRF
Sbjct: 181 TPRRWCAAGSLRGSVYVASGIGSFFSTDVARSVERWDLKPIDGG---AWEKVSGLKDGRF 240

Query: 241 SRDAIDAVGWRGKLCMVNVKGDAAKEGAVYDLEKDEWEEMPEGMIGGWRGPVAAMDEEVM 300
           SRDA DAVGWRGKLCMVNVKGDA KEG VYDLE+DEWEEM EGMIGGWRGPVA M+EE M
Sbjct: 241 SRDATDAVGWRGKLCMVNVKGDARKEGLVYDLERDEWEEMSEGMIGGWRGPVATMEEEEM 300

Query: 301 YVVEEGSGSFRRYDPERDLWEEMMESNRLKGAVQMAAGGGRACVVCGGGSRGEIAVVDVA 360
           YVV+E +GS RRYDPERD WEE+ ESNRLKGAVQMAAGGGR CVVC G SR EI VVDV 
Sbjct: 301 YVVDEVTGSLRRYDPERDFWEEVTESNRLKGAVQMAAGGGRVCVVCSG-SRREIVVVDVV 360

Query: 361 ASPPRLWFEPAPAGMEVVAVHVLPRM 383
           ASPPRLW E  P GMEVVAVHVLPRM
Sbjct: 361 ASPPRLWVEATPPGMEVVAVHVLPRM 366

BLAST of MC10g1386 vs. NCBI nr
Match: XP_022937172.1 (F-box/kelch-repeat protein SKIP25-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 568 bits (1465), Expect = 2.80e-201
Identity = 292/386 (75.65%), Postives = 318/386 (82.38%), Query Frame = 0

Query: 1   MVTAATSPAVPFDSPSKRRKLALHHR---PSPPLLPDLPDDVAHFCLSHLPPSLLFSVCR 60
           MVTA TSP+      SKR KLALHH    PSPPLLP LPD VA  CLS LPPSLLFSV R
Sbjct: 1   MVTA-TSPS------SKRNKLALHHHRHCPSPPLLPGLPDHVAQLCLSRLPPSLLFSVSR 60

Query: 61  SWRRLIYSPSFPPFLSLYAVVSSSSLSPPSNPSPNSLQFFNFDPISAKWNPLPLPPNRPP 120
           SWRRLIYSP+FPPF S+YA++SSS         PNSL FF FDPIS+KWN LP PPN PP
Sbjct: 61  SWRRLIYSPAFPPFFSIYALLSSS---------PNSLDFFTFDPISSKWNRLPPPPNPPP 120

Query: 121 LNLAIRHPSFISRNLPVQSIAVAGKLVLLAATADNFAPALPRPLIFSPLSGSWEFGPPLD 180
           L+L I HPSF+SR+LPVQS+AV+G L+LLAAT  N  PALPRPLIFSP+S SW+FGPPL 
Sbjct: 121 LDLMIHHPSFLSRHLPVQSLAVSGHLLLLAATTQNLLPALPRPLIFSPVSNSWQFGPPLI 180

Query: 181 SPRRWCAAGALRGSVYVASGIGSFFSSDVARSVERWELKPGGGGDRSVWEKVSGLKDGRF 240
           +PRRWCAAGALRGSVYVASGIGSFFS+DVARSVERW+LK   GG    WEKVSGLKD RF
Sbjct: 181 TPRRWCAAGALRGSVYVASGIGSFFSTDVARSVERWDLKKPRGGGGGDWEKVSGLKDRRF 240

Query: 241 SRDAIDAVGWRGKLCMVNVKGDAAKEGAVYDLEKDEWEEMPEGMIGGWRGPVAAMDEEVM 300
           SRDAIDAVGWRGKLCMVNVKGDA KEG VYDLE+D WEEMPEGM+GGWRGPVAAMDEE M
Sbjct: 241 SRDAIDAVGWRGKLCMVNVKGDAPKEGLVYDLERDLWEEMPEGMVGGWRGPVAAMDEEEM 300

Query: 301 YVVEEGSGSFRRYDPERDLWEEMMESNRLKGAVQMAAGGGRACVVCGGGSRGEIAVVDVA 360
           YVV+EG G  RRY+P  DLWE++++SNRLKGAVQMAAGGGR C+VCGG SRGEI VVDV 
Sbjct: 301 YVVDEGGGCLRRYNPVGDLWEQVIQSNRLKGAVQMAAGGGRVCIVCGG-SRGEIVVVDVV 360

Query: 361 ASPPRLWFEPAPAGMEVVAVHVLPRM 383
           ASPP L  EP P GMEVVAVHVLPRM
Sbjct: 361 ASPPWLRVEPTPPGMEVVAVHVLPRM 369

BLAST of MC10g1386 vs. NCBI nr
Match: KAG6596685.1 (F-box/kelch-repeat protein SKIP25, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 568 bits (1465), Expect = 3.24e-201
Identity = 292/390 (74.87%), Postives = 319/390 (81.79%), Query Frame = 0

Query: 1   MVTAATSPAVPFDSPSKRRKLALHHR-------PSPPLLPDLPDDVAHFCLSHLPPSLLF 60
           MVT+ TSP+      SKR KLALHH        PSPPLLP LPD VA  CLS LPPSLLF
Sbjct: 1   MVTS-TSPS------SKRNKLALHHHHHRHRHCPSPPLLPGLPDHVAQLCLSRLPPSLLF 60

Query: 61  SVCRSWRRLIYSPSFPPFLSLYAVVSSSSLSPPSNPSPNSLQFFNFDPISAKWNPLPLPP 120
           SV RSWRRLIYSP+FPPF S+YA++SSS         PNSL FF FDPIS+KWN LP PP
Sbjct: 61  SVSRSWRRLIYSPAFPPFFSIYALLSSS---------PNSLDFFTFDPISSKWNRLPPPP 120

Query: 121 NRPPLNLAIRHPSFISRNLPVQSIAVAGKLVLLAATADNFAPALPRPLIFSPLSGSWEFG 180
           N PPL+L I HPSF+SR+LPVQS+AV+G L+LLAAT  N  PALPRPLIFSP+S SW+FG
Sbjct: 121 NPPPLDLMIHHPSFLSRHLPVQSLAVSGHLLLLAATTQNLLPALPRPLIFSPVSNSWQFG 180

Query: 181 PPLDSPRRWCAAGALRGSVYVASGIGSFFSSDVARSVERWELKPGGGGDRSVWEKVSGLK 240
           PPL +PRRWCAAGALRGSVYVASGIGSFFS+DVARSVERW+LK   GG R  WEKVSGLK
Sbjct: 181 PPLITPRRWCAAGALRGSVYVASGIGSFFSTDVARSVERWDLKKPRGGGRGDWEKVSGLK 240

Query: 241 DGRFSRDAIDAVGWRGKLCMVNVKGDAAKEGAVYDLEKDEWEEMPEGMIGGWRGPVAAMD 300
           D RFSRDAIDAVGWRGKLCMVNVKGDA KEG VYDLE+D WEEMPEGM+GGWRGPVAAMD
Sbjct: 241 DRRFSRDAIDAVGWRGKLCMVNVKGDAPKEGLVYDLERDLWEEMPEGMVGGWRGPVAAMD 300

Query: 301 EEVMYVVEEGSGSFRRYDPERDLWEEMMESNRLKGAVQMAAGGGRACVVCGGGSRGEIAV 360
           EE MYVV+EG G  RRY+P  DLWE++++SNRLKGAVQMAAGGGR C+VCGG SRGEI V
Sbjct: 301 EEEMYVVDEGGGCLRRYNPVGDLWEQVIQSNRLKGAVQMAAGGGRVCIVCGG-SRGEIVV 360

Query: 361 VDVAASPPRLWFEPAPAGMEVVAVHVLPRM 383
           VDV ASPP L  EP P GMEVVAVHVLPRM
Sbjct: 361 VDVVASPPWLRVEPTPPGMEVVAVHVLPRM 373

BLAST of MC10g1386 vs. NCBI nr
Match: XP_022923249.1 (F-box/kelch-repeat protein SKIP25-like [Cucurbita moschata] >KAG6577762.1 F-box/kelch-repeat protein SKIP25, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 568 bits (1463), Expect = 5.45e-201
Identity = 287/372 (77.15%), Postives = 307/372 (82.53%), Query Frame = 0

Query: 17  KRRKLALHH-----RPSPPLLPDLPDDVAHFCLSHLPPSLLFSVCRSWRRLIYSPSFPPF 76
           KR KLALHH     RPSPPLLP LPDDVA  CL HLPPSLLFSVCRSWRRL+YSPSFPPF
Sbjct: 10  KRSKLALHHHHHRHRPSPPLLPGLPDDVAQLCLCHLPPSLLFSVCRSWRRLLYSPSFPPF 69

Query: 77  LSLYAVVSSSSLSPPSNPSPNSLQFFNFDPISAKWNPLPLPPNRPPLNLAIRHPSFISRN 136
            SLYA++SSS         P+S+ FFNFDPIS+KW  LP  PN P L L I HPSF+SR+
Sbjct: 70  FSLYALLSSS---------PDSIDFFNFDPISSKWTSLPPHPNPPSLQLLIHHPSFLSRH 129

Query: 137 LPVQSIAVAGKLVLLAATADNFAPALPRPLIFSPLSGSWEFGPPLDSPRRWCAAGALRGS 196
           LPVQS+AV+G L+LLAAT  N  PALPRPL+FSPLS SW F PPL +PRRWCAAGALRGS
Sbjct: 130 LPVQSLAVSGHLLLLAATTHNLLPALPRPLLFSPLSNSWRFAPPLPTPRRWCAAGALRGS 189

Query: 197 VYVASGIGSFFSSDVARSVERWELKPGGGGDRSVWEKVSGLKDGRFSRDAIDAVGWRGKL 256
           VYVASGIGSFFS+DVARSVERW+LKP  GG    WEKVSGLKDGRFSRDA DAVGWRGKL
Sbjct: 190 VYVASGIGSFFSTDVARSVERWDLKPIDGG---AWEKVSGLKDGRFSRDATDAVGWRGKL 249

Query: 257 CMVNVKGDAAKEGAVYDLEKDEWEEMPEGMIGGWRGPVAAMDEEVMYVVEEGSGSFRRYD 316
           CMVNVKGDA KEG VYDLE+DEWEEM EGMIGGWRGPVA M+EE MYVV+E +GS RRYD
Sbjct: 250 CMVNVKGDARKEGLVYDLERDEWEEMSEGMIGGWRGPVATMEEEEMYVVDEVTGSLRRYD 309

Query: 317 PERDLWEEMMESNRLKGAVQMAAGGGRACVVCGGGSRGEIAVVDVAASPPRLWFEPAPAG 376
           PERD WEE+ ESNRLKGAVQMAAGGGR CVVC G SR EI VVDV ASPPRLW E  P G
Sbjct: 310 PERDFWEEVTESNRLKGAVQMAAGGGRVCVVCSG-SRREIVVVDVVASPPRLWVEATPPG 368

Query: 377 MEVVAVHVLPRM 383
           MEVVAVHVLPRM
Sbjct: 370 MEVVAVHVLPRM 368

BLAST of MC10g1386 vs. ExPASy TrEMBL
Match: A0A5A7UHG3 (F-box/kelch-repeat protein SKIP25 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00900 PE=4 SV=1)

HSP 1 Score: 578 bits (1489), Expect = 3.13e-205
Identity = 292/383 (76.24%), Postives = 316/383 (82.51%), Query Frame = 0

Query: 1   MVTAATSPAVPFDSPSKRRKLALHHRPSPPLLPDLPDDVAHFCLSHLPPSLLFSVCRSWR 60
           MVT AT+ + P    SKR KLA H  PS PLLP LPD VA FCLSH+PPSLLFSV RSWR
Sbjct: 1   MVTTATADS-PTPPSSKRNKLAFHQHPSSPLLPGLPDHVAQFCLSHVPPSLLFSVSRSWR 60

Query: 61  RLIYSPSFPPFLSLYAVVSSSSLSPPSNPSPNSLQFFNFDPISAKWNPLPLPPNRPPLNL 120
           RL+YSPSFPPF SLYA++SSSS         NSL FFNFDPIS+KW+PLP PPN P  +L
Sbjct: 61  RLLYSPSFPPFSSLYALLSSSS---------NSLDFFNFDPISSKWSPLPPPPNSPSSHL 120

Query: 121 AIRHPSFISRNLPVQSIAVAGKLVLLAATADNFAPALPRPLIFSPLSGSWEFGPPLDSPR 180
            I HPSF+SR+LPVQS++V+G L+LLAAT  N  PALPRPL+FSP S SW   PPL +PR
Sbjct: 121 LIHHPSFLSRHLPVQSLSVSGHLLLLAATTHNLLPALPRPLLFSPFSNSWRLAPPLPTPR 180

Query: 181 RWCAAGALRGSVYVASGIGSFFSSDVARSVERWELKPGGGGDRSVWEKVSGLKDGRFSRD 240
           RWCAAGALRGSVYVASGIGSFFS+DVARSVERW+LK  G GD   WEKVSGLKDGRFSRD
Sbjct: 181 RWCAAGALRGSVYVASGIGSFFSTDVARSVERWDLKANGAGD---WEKVSGLKDGRFSRD 240

Query: 241 AIDAVGWRGKLCMVNVKGDAAKEGAVYDLEKDEWEEMPEGMIGGWRGPVAAMDEEVMYVV 300
           AIDAVGW+GKLCMVNVKG A KEG VYDLEKDEWE+MPEGMIGGWRGP AAMDEE MYVV
Sbjct: 241 AIDAVGWKGKLCMVNVKGHALKEGLVYDLEKDEWEDMPEGMIGGWRGPAAAMDEEDMYVV 300

Query: 301 EEGSGSFRRYDPERDLWEEMMESNRLKGAVQMAAGGGRACVVCGGGSRGEIAVVDVAASP 360
           +E SGS RRYDPERD WE++MESNRLKGAVQMAAGGGR CVVCGG SRGEI VVDV ASP
Sbjct: 301 DEVSGSLRRYDPERDFWEDVMESNRLKGAVQMAAGGGRVCVVCGG-SRGEIVVVDVVASP 360

Query: 361 PRLWFEPAPAGMEVVAVHVLPRM 383
            RLW EP P G EVVAVHVLPRM
Sbjct: 361 ARLWVEPTPPGTEVVAVHVLPRM 369

BLAST of MC10g1386 vs. ExPASy TrEMBL
Match: A0A1S3BK00 (F-box/kelch-repeat protein SKIP25 OS=Cucumis melo OX=3656 GN=LOC103490713 PE=4 SV=1)

HSP 1 Score: 578 bits (1489), Expect = 3.13e-205
Identity = 292/383 (76.24%), Postives = 316/383 (82.51%), Query Frame = 0

Query: 1   MVTAATSPAVPFDSPSKRRKLALHHRPSPPLLPDLPDDVAHFCLSHLPPSLLFSVCRSWR 60
           MVT AT+ + P    SKR KLA H  PS PLLP LPD VA FCLSH+PPSLLFSV RSWR
Sbjct: 1   MVTTATADS-PTPPSSKRNKLAFHQHPSSPLLPGLPDHVAQFCLSHVPPSLLFSVSRSWR 60

Query: 61  RLIYSPSFPPFLSLYAVVSSSSLSPPSNPSPNSLQFFNFDPISAKWNPLPLPPNRPPLNL 120
           RL+YSPSFPPF SLYA++SSSS         NSL FFNFDPIS+KW+PLP PPN P  +L
Sbjct: 61  RLLYSPSFPPFSSLYALLSSSS---------NSLDFFNFDPISSKWSPLPPPPNSPSSHL 120

Query: 121 AIRHPSFISRNLPVQSIAVAGKLVLLAATADNFAPALPRPLIFSPLSGSWEFGPPLDSPR 180
            I HPSF+SR+LPVQS++V+G L+LLAAT  N  PALPRPL+FSP S SW   PPL +PR
Sbjct: 121 LIHHPSFLSRHLPVQSLSVSGHLLLLAATTHNLLPALPRPLLFSPFSNSWRLAPPLPTPR 180

Query: 181 RWCAAGALRGSVYVASGIGSFFSSDVARSVERWELKPGGGGDRSVWEKVSGLKDGRFSRD 240
           RWCAAGALRGSVYVASGIGSFFS+DVARSVERW+LK  G GD   WEKVSGLKDGRFSRD
Sbjct: 181 RWCAAGALRGSVYVASGIGSFFSTDVARSVERWDLKANGAGD---WEKVSGLKDGRFSRD 240

Query: 241 AIDAVGWRGKLCMVNVKGDAAKEGAVYDLEKDEWEEMPEGMIGGWRGPVAAMDEEVMYVV 300
           AIDAVGW+GKLCMVNVKG A KEG VYDLEKDEWE+MPEGMIGGWRGP AAMDEE MYVV
Sbjct: 241 AIDAVGWKGKLCMVNVKGHALKEGLVYDLEKDEWEDMPEGMIGGWRGPAAAMDEEDMYVV 300

Query: 301 EEGSGSFRRYDPERDLWEEMMESNRLKGAVQMAAGGGRACVVCGGGSRGEIAVVDVAASP 360
           +E SGS RRYDPERD WE++MESNRLKGAVQMAAGGGR CVVCGG SRGEI VVDV ASP
Sbjct: 301 DEVSGSLRRYDPERDFWEDVMESNRLKGAVQMAAGGGRVCVVCGG-SRGEIVVVDVVASP 360

Query: 361 PRLWFEPAPAGMEVVAVHVLPRM 383
            RLW EP P G EVVAVHVLPRM
Sbjct: 361 ARLWVEPTPPGTEVVAVHVLPRM 369

BLAST of MC10g1386 vs. ExPASy TrEMBL
Match: A0A6J1HK01 (F-box/kelch-repeat protein SKIP25-like OS=Cucurbita maxima OX=3661 GN=LOC111465215 PE=4 SV=1)

HSP 1 Score: 571 bits (1472), Expect = 1.05e-202
Identity = 294/386 (76.17%), Postives = 316/386 (81.87%), Query Frame = 0

Query: 1   MVTAATSPAVPFDSPSKRRKLALH---HRPSPPLLPDLPDDVAHFCLSHLPPSLLFSVCR 60
           MVTAAT       +P KR KLALH   HRPSPPLLP LPDDVA  CLSHLPPSLLFSVCR
Sbjct: 1   MVTAAT------PTP-KRSKLALHPHHHRPSPPLLPGLPDDVAQLCLSHLPPSLLFSVCR 60

Query: 61  SWRRLIYSPSFPPFLSLYAVVSSSSLSPPSNPSPNSLQFFNFDPISAKWNPLPLPPNRPP 120
           SWRRL+YSPSFPPF SLYA++SSS         P+S+ FFNFDPIS+KW  LP  PN P 
Sbjct: 61  SWRRLLYSPSFPPFFSLYALLSSS---------PDSIDFFNFDPISSKWTSLPPHPNPPS 120

Query: 121 LNLAIRHPSFISRNLPVQSIAVAGKLVLLAATADNFAPALPRPLIFSPLSGSWEFGPPLD 180
           L L I HPSF+SR+LPVQS+AV+G L+LLAAT  N  PALPRPL+FSPLS SW F PPL 
Sbjct: 121 LQLLIHHPSFLSRHLPVQSLAVSGHLLLLAATTHNLLPALPRPLLFSPLSNSWRFAPPLP 180

Query: 181 SPRRWCAAGALRGSVYVASGIGSFFSSDVARSVERWELKPGGGGDRSVWEKVSGLKDGRF 240
           +PRRWCAAG+LRGSVYVASGIGSFFS+DVARSVERW+LKP  GG    WEKVSGLKDGRF
Sbjct: 181 TPRRWCAAGSLRGSVYVASGIGSFFSTDVARSVERWDLKPIDGG---AWEKVSGLKDGRF 240

Query: 241 SRDAIDAVGWRGKLCMVNVKGDAAKEGAVYDLEKDEWEEMPEGMIGGWRGPVAAMDEEVM 300
           SRDA DAVGWRGKLCMVNVKGDA KEG VYDLE+DEWEEM EGMIGGWRGPVA M+EE M
Sbjct: 241 SRDATDAVGWRGKLCMVNVKGDARKEGLVYDLERDEWEEMSEGMIGGWRGPVATMEEEEM 300

Query: 301 YVVEEGSGSFRRYDPERDLWEEMMESNRLKGAVQMAAGGGRACVVCGGGSRGEIAVVDVA 360
           YVV+E +GS RRYDPERD WEE+ ESNRLKGAVQMAAGGGR CVVC G SR EI VVDV 
Sbjct: 301 YVVDEVTGSLRRYDPERDFWEEVTESNRLKGAVQMAAGGGRVCVVCSG-SRREIVVVDVV 360

Query: 361 ASPPRLWFEPAPAGMEVVAVHVLPRM 383
           ASPPRLW E  P GMEVVAVHVLPRM
Sbjct: 361 ASPPRLWVEATPPGMEVVAVHVLPRM 366

BLAST of MC10g1386 vs. ExPASy TrEMBL
Match: A0A6J1FAD8 (F-box/kelch-repeat protein SKIP25-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111443540 PE=4 SV=1)

HSP 1 Score: 568 bits (1465), Expect = 1.36e-201
Identity = 292/386 (75.65%), Postives = 318/386 (82.38%), Query Frame = 0

Query: 1   MVTAATSPAVPFDSPSKRRKLALHHR---PSPPLLPDLPDDVAHFCLSHLPPSLLFSVCR 60
           MVTA TSP+      SKR KLALHH    PSPPLLP LPD VA  CLS LPPSLLFSV R
Sbjct: 1   MVTA-TSPS------SKRNKLALHHHRHCPSPPLLPGLPDHVAQLCLSRLPPSLLFSVSR 60

Query: 61  SWRRLIYSPSFPPFLSLYAVVSSSSLSPPSNPSPNSLQFFNFDPISAKWNPLPLPPNRPP 120
           SWRRLIYSP+FPPF S+YA++SSS         PNSL FF FDPIS+KWN LP PPN PP
Sbjct: 61  SWRRLIYSPAFPPFFSIYALLSSS---------PNSLDFFTFDPISSKWNRLPPPPNPPP 120

Query: 121 LNLAIRHPSFISRNLPVQSIAVAGKLVLLAATADNFAPALPRPLIFSPLSGSWEFGPPLD 180
           L+L I HPSF+SR+LPVQS+AV+G L+LLAAT  N  PALPRPLIFSP+S SW+FGPPL 
Sbjct: 121 LDLMIHHPSFLSRHLPVQSLAVSGHLLLLAATTQNLLPALPRPLIFSPVSNSWQFGPPLI 180

Query: 181 SPRRWCAAGALRGSVYVASGIGSFFSSDVARSVERWELKPGGGGDRSVWEKVSGLKDGRF 240
           +PRRWCAAGALRGSVYVASGIGSFFS+DVARSVERW+LK   GG    WEKVSGLKD RF
Sbjct: 181 TPRRWCAAGALRGSVYVASGIGSFFSTDVARSVERWDLKKPRGGGGGDWEKVSGLKDRRF 240

Query: 241 SRDAIDAVGWRGKLCMVNVKGDAAKEGAVYDLEKDEWEEMPEGMIGGWRGPVAAMDEEVM 300
           SRDAIDAVGWRGKLCMVNVKGDA KEG VYDLE+D WEEMPEGM+GGWRGPVAAMDEE M
Sbjct: 241 SRDAIDAVGWRGKLCMVNVKGDAPKEGLVYDLERDLWEEMPEGMVGGWRGPVAAMDEEEM 300

Query: 301 YVVEEGSGSFRRYDPERDLWEEMMESNRLKGAVQMAAGGGRACVVCGGGSRGEIAVVDVA 360
           YVV+EG G  RRY+P  DLWE++++SNRLKGAVQMAAGGGR C+VCGG SRGEI VVDV 
Sbjct: 301 YVVDEGGGCLRRYNPVGDLWEQVIQSNRLKGAVQMAAGGGRVCIVCGG-SRGEIVVVDVV 360

Query: 361 ASPPRLWFEPAPAGMEVVAVHVLPRM 383
           ASPP L  EP P GMEVVAVHVLPRM
Sbjct: 361 ASPPWLRVEPTPPGMEVVAVHVLPRM 369

BLAST of MC10g1386 vs. ExPASy TrEMBL
Match: A0A6J1E5V4 (F-box/kelch-repeat protein SKIP25-like OS=Cucurbita moschata OX=3662 GN=LOC111430994 PE=4 SV=1)

HSP 1 Score: 568 bits (1463), Expect = 2.64e-201
Identity = 287/372 (77.15%), Postives = 307/372 (82.53%), Query Frame = 0

Query: 17  KRRKLALHH-----RPSPPLLPDLPDDVAHFCLSHLPPSLLFSVCRSWRRLIYSPSFPPF 76
           KR KLALHH     RPSPPLLP LPDDVA  CL HLPPSLLFSVCRSWRRL+YSPSFPPF
Sbjct: 10  KRSKLALHHHHHRHRPSPPLLPGLPDDVAQLCLCHLPPSLLFSVCRSWRRLLYSPSFPPF 69

Query: 77  LSLYAVVSSSSLSPPSNPSPNSLQFFNFDPISAKWNPLPLPPNRPPLNLAIRHPSFISRN 136
            SLYA++SSS         P+S+ FFNFDPIS+KW  LP  PN P L L I HPSF+SR+
Sbjct: 70  FSLYALLSSS---------PDSIDFFNFDPISSKWTSLPPHPNPPSLQLLIHHPSFLSRH 129

Query: 137 LPVQSIAVAGKLVLLAATADNFAPALPRPLIFSPLSGSWEFGPPLDSPRRWCAAGALRGS 196
           LPVQS+AV+G L+LLAAT  N  PALPRPL+FSPLS SW F PPL +PRRWCAAGALRGS
Sbjct: 130 LPVQSLAVSGHLLLLAATTHNLLPALPRPLLFSPLSNSWRFAPPLPTPRRWCAAGALRGS 189

Query: 197 VYVASGIGSFFSSDVARSVERWELKPGGGGDRSVWEKVSGLKDGRFSRDAIDAVGWRGKL 256
           VYVASGIGSFFS+DVARSVERW+LKP  GG    WEKVSGLKDGRFSRDA DAVGWRGKL
Sbjct: 190 VYVASGIGSFFSTDVARSVERWDLKPIDGG---AWEKVSGLKDGRFSRDATDAVGWRGKL 249

Query: 257 CMVNVKGDAAKEGAVYDLEKDEWEEMPEGMIGGWRGPVAAMDEEVMYVVEEGSGSFRRYD 316
           CMVNVKGDA KEG VYDLE+DEWEEM EGMIGGWRGPVA M+EE MYVV+E +GS RRYD
Sbjct: 250 CMVNVKGDARKEGLVYDLERDEWEEMSEGMIGGWRGPVATMEEEEMYVVDEVTGSLRRYD 309

Query: 317 PERDLWEEMMESNRLKGAVQMAAGGGRACVVCGGGSRGEIAVVDVAASPPRLWFEPAPAG 376
           PERD WEE+ ESNRLKGAVQMAAGGGR CVVC G SR EI VVDV ASPPRLW E  P G
Sbjct: 310 PERDFWEEVTESNRLKGAVQMAAGGGRVCVVCSG-SRREIVVVDVVASPPRLWVEATPPG 368

Query: 377 MEVVAVHVLPRM 383
           MEVVAVHVLPRM
Sbjct: 370 MEVVAVHVLPRM 368

BLAST of MC10g1386 vs. TAIR 10
Match: AT1G31350.1 (KAR-UP F-box 1 )

HSP 1 Score: 371.3 bits (952), Expect = 9.2e-103
Identity = 204/389 (52.44%), Postives = 265/389 (68.12%), Query Frame = 0

Query: 3   TAATSPAVPFDSPSKRRKLALHHRPSPPLLPDLPDDVAHFCLSHL-PPSLLFSVCRSWRR 62
           TAA SP      P+KRR+    +  S  L+  LPD ++  CLS +  PSLL +VC  WRR
Sbjct: 14  TAAESP------PAKRRRTVTGNENS-ALIEGLPDHISEICLSLVHRPSLLSAVCTRWRR 73

Query: 63  LIYSPSFPPFLSLYAV-VSSSSLSPPSNPSPNSLQFFNFDPISAKWNPLPLPPNRPPLN- 122
           L+YSP FP F SLYA+ V S+S +   NP   S++F  F+P+S+KW PLP PP  PPL+ 
Sbjct: 74  LLYSPEFPSFPSLYALFVDSTSDTGRVNP---SVRFMCFNPVSSKWYPLPPPPPDPPLHR 133

Query: 123 LAIRHPSFISRNLPVQSIAVAGKLVLLAATADNFAPALPRPLIFSPLSGSWEFGPPLDSP 182
           +  RHPSFIS NLP+Q ++ AGKL+L+A +    +PA+  PLIF P+S SW  GP + SP
Sbjct: 134 ILYRHPSFISFNLPIQCVSAAGKLILIAGSNQQLSPAISHPLIFDPISSSWSSGPRIGSP 193

Query: 183 RRWCAAGALRGSVYVASGIGSFFSSDVARSVERWEL-KPGGGGDRSVWEKVSGLKDGRFS 242
           RRWCA GA  G++Y+ASGI S FSS VA+SVE+ +L +      R  WEK+  ++D RFS
Sbjct: 194 RRWCATGACDGAIYIASGISSQFSSTVAKSVEKLDLTEQNRNNHRFNWEKLRDMRDLRFS 253

Query: 243 RDAIDAVGWRGKLCMVNVKGDAAKEGAVYDLEKDEWEEMPEGMIGGWRGPVAAMDEEVMY 302
           R+AIDAVG+R KL MVNVKGDA KEGA+YD+ KD+WE MPE M+ GWRGPVAAM+EE++Y
Sbjct: 254 REAIDAVGYRRKLLMVNVKGDAVKEGAIYDVVKDDWEPMPEEMLVGWRGPVAAMEEEILY 313

Query: 303 VVEEGSGSFRRYDPERDLWEEMM----ESNRLKGAVQMAAGGGRACVVCGGGSRGEIAVV 362
            V+E  G+ R+YD E+  W E++        LKGA Q+ A  G+ CVV G    G+I VV
Sbjct: 314 SVDERRGTVRKYDDEKREWREVVVVEGGEEMLKGATQVTADSGKLCVVTGD---GKIVVV 373

Query: 363 DVAASPPRLWFEPAPAGMEVVAVHVLPRM 384
           DVAA P ++W    P G+E V+VHVLPRM
Sbjct: 374 DVAAEPAKIWNVEIPDGLEPVSVHVLPRM 389

BLAST of MC10g1386 vs. TAIR 10
Match: AT2G24540.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 81.3 bits (199), Expect = 1.9e-15
Identity = 92/380 (24.21%), Postives = 146/380 (38.42%), Query Frame = 0

Query: 28  SPPLLPDLPDDVAHFCLSHLP---PSLLFSVCRSWRRLIYSPSFPPFLSLYAVVSSSSLS 87
           S PL+  LP+D+A  CL  LP    +L  SV  SW + I +P F     L++  S S  S
Sbjct: 25  SQPLISGLPNDIAELCLLRLPYPYHALYRSVSSSWNKTITNPRF-----LFSKQSLSISS 84

Query: 88  P-----PSNPSPNSLQFFNFDPISAKWNPLPLPPNRPPLNLAIRHPSFISRNLPVQSIAV 147
           P       N S   +Q+ + D  S +W  LP  PN       I  P  +S      S+  
Sbjct: 85  PYLFVFAFNKSTARIQWQSLDLASGRWFVLPPMPNS---FTKISSPHALS----CASMPR 144

Query: 148 AGKLVLLAATADNFAPALPRPLIFSPLSGSWEFGPPLDSPRRWCAAGALRGSVYVASG-I 207
            GKL +L     N +      ++++ L+  W    P+ SPR +  +G + G +    G +
Sbjct: 145 QGKLFVLGGGDVNRS-----AVVYTALTNRWSCISPMMSPRTYFVSGNVNGKIMAVGGSV 204

Query: 208 GSFFSSDVARSVERWELKPGGGGDRSVWEKVSGL-------KDGRFSRDAIDAVGWRGKL 267
           G   + +    VE ++       D   W  V  L             ++     GW    
Sbjct: 205 GG--NGEATTEVESYD------PDNDTWTVVKKLPMVLAKYDSAVIGKEMCVTEGWAWPF 264

Query: 268 CMVNVKGDAAKEGAVYDLEKDEWEEMPEGMIGGWRGPVAAMDEEVMYVVEEGSGSFRRYD 327
               +       G VYD ++  W EM  GM  GW G    + + +  + E G    + Y 
Sbjct: 265 MFPPM-------GQVYDSDEGTWREMSGGMKEGWTGVSVVIRDRLFVISEHGDFPMKVYC 324

Query: 328 PERDLWE----EMMESNRLKGAVQMAAGGGRACVVCGG----------GSRGEIAVVDVA 378
            + D W     E ++  +++    +     R  VV  G          G  G+ +V    
Sbjct: 325 SDDDTWRYVSGEKLQGEKMRRPFAVTGADDRVFVVASGINVAEGRVSEGQNGDFSVEWRM 372

BLAST of MC10g1386 vs. TAIR 10
Match: AT1G55270.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 73.6 bits (179), Expect = 4.0e-13
Identity = 80/355 (22.54%), Postives = 133/355 (37.46%), Query Frame = 0

Query: 26  RPSPPLLPDLPDDVAHFCLSHLPPS---LLFSVCRSWRRLIYSPSFPPFLSLYAVVSSSS 85
           R  PPLLP LPDD+A  CL  +P +    L  VC+ W RL     F     L   +S   
Sbjct: 73  RIQPPLLPGLPDDLAVACLIRVPRAEHRKLRLVCKRWYRLASGNFFYSQRKLLG-MSEEW 132

Query: 86  LSPPSNPSPNSLQFFNFDPISAKWNPLPLPPNRPPLNLAIRHPSFISRNLPVQSIAVAGK 145
           +          + +  FDPIS  W PLP  P               S  +      ++G 
Sbjct: 133 VYVFKRDRDGKISWNTFDPISQLWQPLPPVPRE------------YSEAVGFGCAVLSGC 192

Query: 146 LVLLAATADNFAPALPRPLIFSPLSGSWEFGPPLDSPRRWCAAGALRGSVYVASGIGSFF 205
            + L    D    ++ R + ++  +  W   P +   R +     +   +YVA G     
Sbjct: 193 HLYLFGGKDPLRGSMRRVIFYNARTNKWHRAPDMLRKRHFFGCCVINNCLYVAGGECEGI 252

Query: 206 SSDVARSVERWELKPGGGGDRSVWEKVSGLKDGRFSRDAIDAVGWRGKLCMVNVKGDAAK 265
              + RS E ++       +++ W   S + D   +   +  V +  K  +  +      
Sbjct: 253 QRTL-RSAEVYD------PNKNRW---SFIADMSTAMVPLIGVVYDKKWFLKGLGSHQLV 312

Query: 266 EGAVYDLEKDEWEEMPEGMIGGWRGPVAAMDEEVMYVVEEGSGSFRRYDPERDLWEEMME 325
               YD E + W  + +GM+ GWR P  +++  +  +        R +D   D W + M+
Sbjct: 313 MSEAYDPEVNSWSPVSDGMVAGWRNPCTSLNGRLYGLDCRDGCKLRVFDESTDSWNKFMD 372

Query: 326 SNRLKG------AVQMAAGGGRACVVCGGGSRGEIAVVDVAASPPRLWFEPAPAG 372
           S    G      A  +     + C++    S   + V +   + PRLW   A  G
Sbjct: 373 SKAHLGNSKSLEAAALVPLHNKLCIIRNNMSMSLVDVSNPDKNNPRLWENIAVKG 404

BLAST of MC10g1386 vs. TAIR 10
Match: AT1G30090.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 60.1 bits (144), Expect = 4.6e-09
Identity = 76/303 (25.08%), Postives = 116/303 (38.28%), Query Frame = 0

Query: 30  PLLPDLPDDVAHFCLSHLPPSLLF---SVCRSWRRLIYSPSFPPFLSLYAVVSSSSLSPP 89
           PL+P LPDDVA  CL  +P        SVC+ W  L     F    + +A         P
Sbjct: 52  PLIPGLPDDVALNCLLRVPVQSHVSSKSVCKRWHLL-----FGTKETFFAKRKEFGFKDP 111

Query: 90  ------SNPSPNSLQFFNFDPISAKWNPLPLPPNRPPLNLAIRHPSFISRNLPVQ-SIAV 149
                  +     +Q+   D  +  W+ +P  P R      +    F S ++P + ++ V
Sbjct: 112 WLFVVGFSRCTGKIQWKVLDLRNLTWHEIPAMPCRD----KVCPHGFRSVSMPREGTMFV 171

Query: 150 AGKLVLLAATADNFAPALPRPLIFSPLSGSWEFGPPLDSPRRWCAAGALRGSVYVASG-I 209
            G +V     +D+  P L   L +  +   W     + + R + A+G + G +Y A G  
Sbjct: 172 CGGMV-----SDSDCP-LDLVLKYDMVKNHWTVTNKMITARSFFASGVIDGMIYAAGGNA 231

Query: 210 GSFFSSDVARSVERWELKPGGGGDRSVWEKVSGLKDGRFSRDAIDAVGWRGKLCMVN--- 269
              +  D A       L P  G  R V   V+ +        + D     GKL +     
Sbjct: 232 ADLYELDCAE-----VLNPLDGNWRPVSNMVAHMA-------SYDTAVLNGKLLVTEGWL 291

Query: 270 VKGDAAKEGAVYDLEKDEWEEMPEGMIGGWRGPVAAMDEEVMYVVEEGSGSFRRYDPERD 319
                +  G VYD   D+WE M  G+  GW G    + + +  V E      + YDP  D
Sbjct: 292 WPFFVSPRGQVYDPRTDQWETMSMGLREGWTGTSVVIYDRLFIVSELERMKMKVYDPVTD 327

BLAST of MC10g1386 vs. TAIR 10
Match: AT1G22040.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 48.9 bits (115), Expect = 1.1e-05
Identity = 92/379 (24.27%), Postives = 139/379 (36.68%), Query Frame = 0

Query: 5   ATSPAVPFDSPS-KRRKLALHHRPSP--PLLPDLPDDVAHFCLSHLPP---SLLFSVCRS 64
           ATS  V   S S KRRK++  +       L+P LPD+++   L+ LP    S +  V R 
Sbjct: 14  ATSQDVECSSESRKRRKISSENDEEECCRLIPSLPDELSIQILARLPRICYSSVRLVSRR 73

Query: 65  WRRLIYSPSFPPFLSLYAVVSSSSLSPPSNPSPNSLQFFNFDPISAKWNPLPLPP----- 124
           WR  + S S    L      +   L   +    + L ++  DP+S KW  LP  P     
Sbjct: 74  WRSAV-STSEVYSLRKELGRTEEWLYVLTKGHEDKLLWYALDPVSTKWQRLPPMPVVVYE 133

Query: 125 -------------NRPPLNLAIRHPSFISR-----NLPVQSIAV----AGKLVLLAATAD 184
                          P  N+     SF+ R      +P    A+     G  V+   +  
Sbjct: 134 EESRKSLSGLWNMITPSFNVGAIVRSFLGRRDSSEQMPFCGCAIGAVDGGLYVIGGLSRS 193

Query: 185 NFAPALPRPLIFSPLSGSWEFGPPLDSPRRWCAAGALRGSVYVASGI-------GSFFSS 244
                + R   F P+  SW     + + R +   G L   +YV  G+           S+
Sbjct: 194 KTVSCVWR---FDPILNSWSEVSSMLASRAYSKTGVLNKKLYVVGGVDRGRGGLSPLQSA 253

Query: 245 DV-ARSVERWELKPGGGGDRSVWEKVSGLKDGRFSRDAIDAVG-----WRGKLCM----- 304
           +V   S + W   P        + K   L +  F  D +  +      + G+LC+     
Sbjct: 254 EVYDPSTDAWSEVPS-----MPFSKAQVLPNA-FLADLLKPIATGMTCYNGRLCVPQSLY 313

Query: 305 -----VNVKGDAAKEGAVYDLEKDEWEEMPEGMIGGWRGPVAAMDEEV-----MYVVEEG 319
                V+V G+      VYD E + W EMP GM  GW    A     V     +Y  +  
Sbjct: 314 SWPFFVDVGGE------VYDPETNLWVEMPSGMGEGWPARQAGTKLSVVVDGELYAFDPS 373

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GX291.3e-10152.44F-box/kelch-repeat protein SKIP25 OS=Arabidopsis thaliana OX=3702 GN=SKIP25 PE=1... [more]
Q8LAW22.7e-1424.21F-box protein AFR OS=Arabidopsis thaliana OX=3702 GN=AFR PE=1 SV=2[more]
Q93W935.6e-1222.54F-box/kelch-repeat protein At1g55270 OS=Arabidopsis thaliana OX=3702 GN=At1g5527... [more]
Q9C6Z06.4e-0825.08F-box/kelch-repeat protein At1g30090 OS=Arabidopsis thaliana OX=3702 GN=At1g3009... [more]
Q9LM551.5e-0424.27F-box/kelch-repeat protein At1g22040 OS=Arabidopsis thaliana OX=3702 GN=At1g2204... [more]
Match NameE-valueIdentityDescription
XP_008448576.16.46e-20576.24PREDICTED: F-box/kelch-repeat protein SKIP25 [Cucumis melo] >KAA0052979.1 F-box/... [more]
XP_022965312.12.16e-20276.17F-box/kelch-repeat protein SKIP25-like [Cucurbita maxima][more]
XP_022937172.12.80e-20175.65F-box/kelch-repeat protein SKIP25-like isoform X2 [Cucurbita moschata][more]
KAG6596685.13.24e-20174.87F-box/kelch-repeat protein SKIP25, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022923249.15.45e-20177.15F-box/kelch-repeat protein SKIP25-like [Cucurbita moschata] >KAG6577762.1 F-box/... [more]
Match NameE-valueIdentityDescription
A0A5A7UHG33.13e-20576.24F-box/kelch-repeat protein SKIP25 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3BK003.13e-20576.24F-box/kelch-repeat protein SKIP25 OS=Cucumis melo OX=3656 GN=LOC103490713 PE=4 S... [more]
A0A6J1HK011.05e-20276.17F-box/kelch-repeat protein SKIP25-like OS=Cucurbita maxima OX=3661 GN=LOC1114652... [more]
A0A6J1FAD81.36e-20175.65F-box/kelch-repeat protein SKIP25-like isoform X2 OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1E5V42.64e-20177.15F-box/kelch-repeat protein SKIP25-like OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
Match NameE-valueIdentityDescription
AT1G31350.19.2e-10352.44KAR-UP F-box 1 [more]
AT2G24540.11.9e-1524.21Galactose oxidase/kelch repeat superfamily protein [more]
AT1G55270.14.0e-1322.54Galactose oxidase/kelch repeat superfamily protein [more]
AT1G30090.14.6e-0925.08Galactose oxidase/kelch repeat superfamily protein [more]
AT1G22040.11.1e-0524.27Galactose oxidase/kelch repeat superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015915Kelch-type beta propellerGENE3D2.120.10.80coord: 74..352
e-value: 2.6E-20
score: 74.5
IPR015915Kelch-type beta propellerSUPERFAMILY117281Kelch motifcoord: 92..354
NoneNo IPR availablePANTHERPTHR47590:SF1F-BOX/KELCH-REPEAT PROTEIN SKIP25coord: 14..383
NoneNo IPR availablePANTHERPTHR47590F-BOX/KELCH-REPEAT PROTEIN SKIP25coord: 14..383
IPR036047F-box-like domain superfamilySUPERFAMILY81383F-box domaincoord: 26..68

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC10g1386.1MC10g1386.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding