Homology
BLAST of MC10g1035 vs. ExPASy Swiss-Prot
Match:
F4I6M1 (DNA polymerase I A, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=POLIA PE=2 SV=1)
HSP 1 Score: 1181.8 bits (3056), Expect = 0.0e+00
Identity = 582/833 (69.87%), Postives = 686/833 (82.35%), Query Frame = 0
Query: 302 VEEFSEITING--TGTKMMEAQANDHKPDIKERLNSVYDSVLVVDSVSAAKEVVSMLTTK 361
+ + S T NG + + + + +++E L +YD VL+VD+V AAK+ V+ L +
Sbjct: 226 ISDKSSGTANGNKNTVAISKVERSTEPSNVRENLGKIYDKVLIVDNVQAAKDTVAKLVNQ 285
Query: 362 YKNLVHACDTEVAKIDVKQETPVDHGEIICFSIYSGPNANFGSGKSCIWVDVLDGGGKEI 421
++N VH+CDTEV+ I+VK+ETPVDHGE+ICFSIY GP A+FG+GKSCIWVDVL G+E+
Sbjct: 286 FRNHVHSCDTEVSGIEVKEETPVDHGELICFSIYCGPEADFGNGKSCIWVDVLGENGREV 345
Query: 422 LLQFAPFFEDPLIKKVWHNYSFDNHIIENYGIKVSGFHADTMHMARLWDSSRRANGGYSL 481
L +F P+FED I+KVWHNYSFD+HII N+GI++SGFHADTMHMARLWDS+RR GGYSL
Sbjct: 346 LAEFKPYFEDSFIRKVWHNYSFDSHIIRNHGIEISGFHADTMHMARLWDSARRIKGGYSL 405
Query: 482 EALSGDVKVMSGAKLGHEKELIGKVSMKTIFGRKKMKKDGYEGKLTVIPPVEELQREERR 541
EAL+ D KV+ G + E E +GK+SMKTIFG++K+KKDG EGK+ VIPPVEELQRE+R
Sbjct: 406 EALTSDPKVLGGTQTKEEAEFLGKISMKTIFGKRKLKKDGSEGKIVVIPPVEELQREDRE 465
Query: 542 PWVSYSALDSICTLKLYESLKNKLSNMPWERDGEMIPDKTMFNFYEEYWQPFGELLVKME 601
W+SYSALD+I TLKLYES+ KL M W DG+ + +TM +FY E+W+PFGELLVKME
Sbjct: 466 AWISYSALDAISTLKLYESMTKKLQLMDWHLDGKPVLGRTMLDFYHEFWRPFGELLVKME 525
Query: 602 TEGMLVDRAYLAEIEKLAKAEQEVAGNRFRNWASKYCPDAKYMNVGSDAQVRQLLFGGTL 661
EG+LVDR YLAEIEK+AKAEQ+VAG+RFRNWASKYCPDAKYMN+GSD Q+RQL FGG
Sbjct: 526 AEGILVDREYLAEIEKVAKAEQQVAGSRFRNWASKYCPDAKYMNIGSDTQLRQLFFGGIS 585
Query: 662 NSKNPDESLPAERTFKVPNSENVIEEGKKTPGKFRNITLQSILKDKVLSTEMYTASGWPS 721
NS DE LP E+ FKVPN + VIEEGKKTP KFRNI L I D LSTE +TASGWPS
Sbjct: 586 NSH--DEVLPVEKLFKVPNIDKVIEEGKKTPTKFRNIKLHRI-SDSPLSTENFTASGWPS 645
Query: 722 VSGDALKILAGKVSAEFDDFTDAHDDLQSDNEVDNDSETMPHGENKKPVIHESANMSDYG 781
V GD LK LAGKVSAE+D D D + D+D ET E +K + + S YG
Sbjct: 646 VGGDVLKELAGKVSAEYDFMDDVSDISLEEVVEDDDVET---SETQKSKTDDETDTSAYG 705
Query: 782 TAFEAFASKEEGREACHAIAALCEVCSIDSLISNFILPLQGSNISGKNGRVHCSLNINTE 841
TA+ AF E G+EACHAIA+LCEVCSIDSLISNFILPLQGSN+SGK+GRVHCSLNINTE
Sbjct: 706 TAYVAFGGGERGKEACHAIASLCEVCSIDSLISNFILPLQGSNVSGKDGRVHCSLNINTE 765
Query: 842 TGRLSARRPNLQNQPALEKDRYKIRQAFIAAPGNSLIVADYGQLELRILAHLANCKSMLE 901
TGRLSARRPNLQNQPALEKDRYKIR+AF+A+PGN+L+VADYGQLELRILAHL CKSM+E
Sbjct: 766 TGRLSARRPNLQNQPALEKDRYKIRKAFVASPGNTLVVADYGQLELRILAHLTGCKSMME 825
Query: 902 AFKAGGDFHSRTAMNMYPHIRNAVEKGSVLLEWDPQPGEDKPPVPLLKDAFGSERRKAKM 961
AFKAGGDFHSRTAMNMYPH+R AVE G V+LEW P+PGEDKPPVPLLKDAFGSERRKAKM
Sbjct: 826 AFKAGGDFHSRTAMNMYPHVREAVENGQVILEWHPEPGEDKPPVPLLKDAFGSERRKAKM 885
Query: 962 LNFSIAYGKTPVGLSKDWKVTVEEARQTVDLWYNERKEVRIWQELRKKEADEKSCVRTLL 1021
LNFSIAYGKT VGLS+DWKV+ +EA++TVDLWYN+R+EVR WQE+RKKEA E V TLL
Sbjct: 886 LNFSIAYGKTAVGLSRDWKVSTKEAQETVDLWYNDRQEVRKWQEMRKKEAIEDGYVLTLL 945
Query: 1022 GRARRFPSMKHATRAQRGHIERAAINTPVQGSAADVAMCAMLEISNNSGLRELGWRLLLQ 1081
GR+RRFP+ K +RAQR HI+RAAINTPVQGSAADVAMCAMLEIS N L++LGWRLLLQ
Sbjct: 946 GRSRRFPASK--SRAQRNHIQRAAINTPVQGSAADVAMCAMLEISINQQLKKLGWRLLLQ 1005
Query: 1082 VHDEVILEGPTESAEVAKAIVVECMSKPFSGKNILNVDLAVDAKCAQNWYSAK 1133
+HDEVILEGP ESAE+AK IVV+CMSKPF+G+NIL+VDL+VDAKCAQNWY+AK
Sbjct: 1006 IHDEVILEGPIESAEIAKDIVVDCMSKPFNGRNILSVDLSVDAKCAQNWYAAK 1050
BLAST of MC10g1035 vs. ExPASy Swiss-Prot
Match:
Q84ND9 (DNA polymerase I B, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=POLIB PE=2 SV=1)
HSP 1 Score: 1167.1 bits (3018), Expect = 0.0e+00
Identity = 585/806 (72.58%), Postives = 672/806 (83.37%), Query Frame = 0
Query: 329 IKERLNSVYDSVLVVDSVSAAKEVVSMLTTKYKNLVHACDTEVAKIDVKQETPVDHGEII 388
++ L +Y+ V VVD+VS+AKE V++L +Y+NLVHACDTEV++IDVK ETPVDHGE+I
Sbjct: 233 VRANLKKIYNRVRVVDNVSSAKETVALLMNQYRNLVHACDTEVSRIDVKTETPVDHGEMI 292
Query: 389 CFSIYSGPNANFGSGKSCIWVDVLDGGGKEILLQFAPFFEDPLIKKVWHNYSFDNHIIEN 448
CFSIY G A+FG GKSCIWVDVL G++IL +F PFFED IKKVWHNYSFDNHII N
Sbjct: 293 CFSIYCGSEADFGDGKSCIWVDVLGENGRDILAEFKPFFEDSSIKKVWHNYSFDNHIIRN 352
Query: 449 YGIKVSGFHADTMHMARLWDSSRRANGGYSLEALSGDVKVMSGAKLGHEKELIGKVSMKT 508
YGIK+SGFH DTMHMARLWDSSRR +GGYSLEAL+ D KV+ G + E EL GK+SMK
Sbjct: 353 YGIKLSGFHGDTMHMARLWDSSRRISGGYSLEALTSDPKVLGGTETKEEAELFGKISMKK 412
Query: 509 IFGRKKMKKDGYEGKLTVIPPVEELQREERRPWVSYSALDSICTLKLYESLKNKLSNMPW 568
IFG+ K+KKDG EGKL +IPPV+ELQ E+R W+SYSALDSI TLKLYES+K +L W
Sbjct: 413 IFGKGKLKKDGSEGKLVIIPPVKELQMEDREAWISYSALDSISTLKLYESMKKQLQAKKW 472
Query: 569 ERDGEMIPDKTMFNFYEEYWQPFGELLVKMETEGMLVDRAYLAEIEKLAKAEQEVAGNRF 628
DG++I K MF+FY+EYWQPFGELL KME+EGMLVDR YLA+IE +AKAEQE+A +RF
Sbjct: 473 FLDGKLISKKNMFDFYQEYWQPFGELLAKMESEGMLVDRDYLAQIEIVAKAEQEIAVSRF 532
Query: 629 RNWASKYCPDAKYMNVGSDAQVRQLLFGGTLNSKNPDESLPAERTFKVPNSENVIEEGKK 688
RNWASK+CPDAK+MNVGSD Q+RQL FGG NS N DE LP E+ FKVPN + VIEEGKK
Sbjct: 533 RNWASKHCPDAKHMNVGSDTQLRQLFFGGISNSCN-DEDLPYEKLFKVPNVDKVIEEGKK 592
Query: 689 TPGKFRNITLQSILKDKVLSTEMYTASGWPSVSGDALKILAGKVSAEFDDFTDAHDDLQS 748
KFRNI L I D+ L TE +TASGWPSVSGD LK LAGKVSAE+D D
Sbjct: 593 RATKFRNIKLHRI-SDRPLPTEKFTASGWPSVSGDTLKALAGKVSAEYDYMEGVLDTCLE 652
Query: 749 DNEVDNDSETMPHGENKKPVIHES--ANMSDYGTAFEAFASKEEGREACHAIAALCEVCS 808
+N D+D ++P + ++ S ++ S YGTAF+AF E G+EACHAIAALCEVCS
Sbjct: 653 ENIGDDDCISLPDEVVETQHVNTSVESDTSAYGTAFDAFGGGESGKEACHAIAALCEVCS 712
Query: 809 IDSLISNFILPLQGSNISGKNGRVHCSLNINTETGRLSARRPNLQNQPALEKDRYKIRQA 868
IDSLISNFILPLQGSN+SGK+GRVHCSLNINTETGRLSARRPNLQNQPALEKDRYKIRQA
Sbjct: 713 IDSLISNFILPLQGSNVSGKDGRVHCSLNINTETGRLSARRPNLQNQPALEKDRYKIRQA 772
Query: 869 FIAAPGNSLIVADYGQLELRILAHLANCKSMLEAFKAGGDFHSRTAMNMYPHIRNAVEKG 928
FIA+PGNSLIVADYGQLELRILAHLA+C+SM EAF AGGDFHSRTAMNMYPHIR AVE G
Sbjct: 773 FIASPGNSLIVADYGQLELRILAHLASCESMKEAFIAGGDFHSRTAMNMYPHIREAVENG 832
Query: 929 SVLLEWDPQPGEDKPPVPLLKDAFGSERRKAKMLNFSIAYGKTPVGLSKDWKVTVEEARQ 988
VLLEW PQPG++KPPVPLLKDAF SERRKAKMLNFSIAYGKT +GLS+DWKV+ EEA+
Sbjct: 833 EVLLEWHPQPGQEKPPVPLLKDAFASERRKAKMLNFSIAYGKTAIGLSRDWKVSREEAQD 892
Query: 989 TVDLWYNERKEVRIWQELRKKEADEKSCVRTLLGRARRFPSMKHATRAQRGHIERAAINT 1048
TV+LWYN+R+EVR WQELRKKEA +K V TLLGRAR+FP ++ +RAQ+ HIERAAINT
Sbjct: 893 TVNLWYNDRQEVRKWQELRKKEAIQKGYVLTLLGRARKFP--EYRSRAQKNHIERAAINT 952
Query: 1049 PVQGSAADVAMCAMLEISNNSGLRELGWRLLLQVHDEVILEGPTESAEVAKAIVVECMSK 1108
PVQGSAADVAMCAMLEISNN L+ELGW+LLLQVHDEVILEGP+ESAE AK IVV CMS+
Sbjct: 953 PVQGSAADVAMCAMLEISNNQRLKELGWKLLLQVHDEVILEGPSESAENAKDIVVNCMSE 1012
Query: 1109 PFSGKNILNVDLAVDAKCAQNWYSAK 1133
PF+GKNIL+VDL+VDAKCAQNWY+ K
Sbjct: 1013 PFNGKNILSVDLSVDAKCAQNWYAGK 1034
BLAST of MC10g1035 vs. ExPASy Swiss-Prot
Match:
Q6Z4T5 (DNA polymerase I A, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g0175300 PE=2 SV=1)
HSP 1 Score: 1073.9 bits (2776), Expect = 1.1e-312
Identity = 564/871 (64.75%), Postives = 661/871 (75.89%), Query Frame = 0
Query: 269 NGRKQNI----STGKVNNVPKSLKFIEASNEIKRGVDVEEFSEITINGTGTKMMEAQAND 328
+G K+ I S +++PK K + K D +E IT T +A +
Sbjct: 183 DGSKEEIPWHDSVTMESSLPKVSKSEKTLVVDKAIPDKKEHKRITRKVTPNFPDKASLST 242
Query: 329 HKPDIKERLNSVYDSVLVVDSVSAAKEVVSMLTTKYKNLVHACDTEVAKIDVKQETPVDH 388
+ ++ L ++YD VLVVD+V +A+ VV +LTTKYK +HACDTEVA IDVK+ETPV H
Sbjct: 243 ESKNARKLLATIYDKVLVVDNVESARSVVKLLTTKYKGFIHACDTEVANIDVKEETPVGH 302
Query: 389 GEIICFSIYSGPN---ANFGSGKSCIWVDVLDGGGKEILLQFAPFFEDPLIKKVWHNYSF 448
GE+ICFSI SG + A+FG+GK+CIWVDVLD GG+++L++FAPFFEDP IKKVWHNYSF
Sbjct: 303 GEVICFSICSGNSDGEADFGNGKTCIWVDVLD-GGRDVLMEFAPFFEDPFIKKVWHNYSF 362
Query: 449 DNHIIENYGIKVSGFHADTMHMARLWDSSRRANGGYSLEALSGDVKVMSGAKLGHEKELI 508
D H+IEN GIKV+GFHADTMH+ARLWDSSRR +GGYSLE L+ D +VM K
Sbjct: 363 DIHVIENCGIKVAGFHADTMHLARLWDSSRRTDGGYSLEGLTNDYRVMDAVLKDIPK--T 422
Query: 509 GKVSMKTIFGRKKMKKDGYEGKLTVIPPVEELQREERRPWVSYSALDSICTLKLYESLKN 568
GKVSMKTIFGRKK++KDG EGK I PVE+LQRE+R W+ YS+LDS+ TLKLYESLKN
Sbjct: 423 GKVSMKTIFGRKKVRKDGSEGKTISIEPVEKLQREDRELWICYSSLDSMSTLKLYESLKN 482
Query: 569 KLSNMPWERDGEMIPDKTMFNFYEEYWQPFGELLVKMETEGMLVDRAYLAEIEKLAKAEQ 628
KL W D P TM++FYEEYW+PFG LLVKMETEG+LVDRAYL+EIEK A E+
Sbjct: 483 KLEAKEWIFDD--CPRGTMYDFYEEYWRPFGALLVKMETEGVLVDRAYLSEIEKAAVTER 542
Query: 629 EVAGNRFRNWASKYCPDAKYMNVGSDAQVRQLLFGGTLNSKNPDESLPAERTFKVPNSEN 688
E+A ++FR WASK+CPDAKYMNV SD Q+RQL FGG N E+ P +TFKVPN E
Sbjct: 543 ELAADKFRKWASKHCPDAKYMNVNSDNQIRQLFFGGIENRNKRGETWPQSKTFKVPNDEG 602
Query: 689 VIEEGKKTPGKFRNITLQSILKDKVLSTEMYTASGWPSVSGDALKILAGKVSAEFDDFTD 748
+ EGKKTP K R I L +I++D L +M+T +GWPSVSGD L+ LAGK+ TD
Sbjct: 603 IATEGKKTP-KSRTIKLFTIVED--LKIDMFTPTGWPSVSGDVLRSLAGKIP------TD 662
Query: 749 AHDDLQSDNEVDNDSETMPHGENKKPVIHESANMSDYGTAFEAFASKEEGREACHAIAAL 808
+ E D D ++ E + + S YGTA+EAF ++GREACHAIAAL
Sbjct: 663 HIYKIDDGQEFDEDGSSLELPE------QDIEDTSPYGTAYEAFGGGKKGREACHAIAAL 722
Query: 809 CEVCSIDSLISNFILPLQGSNISGKNGRVHCSLNINTETGRLSARRPNLQNQPALEKDRY 868
CEV SID LIS FI+PLQG IS K GR+HCSLNINTETGRLSAR PNLQNQPALEKDRY
Sbjct: 723 CEVFSIDKLISGFIVPLQGDRISCKEGRIHCSLNINTETGRLSARTPNLQNQPALEKDRY 782
Query: 869 KIRQAFIAAPGNSLIVADYGQLELRILAHLANCKSMLEAFKAGGDFHSRTAMNMYPHIRN 928
KIR AF+AAPGN+LIVADYGQLELRILAHL NCKSMLEAFKAGGDFHSRTAMNMY H+R+
Sbjct: 783 KIRHAFVAAPGNTLIVADYGQLELRILAHLTNCKSMLEAFKAGGDFHSRTAMNMYQHVRD 842
Query: 929 AVEKGSVLLEWDPQPGEDKPPVPLLKDAFGSERRKAKMLNFSIAYGKTPVGLSKDWKVTV 988
AVE+ VLLEW PQPG+DKPPVPLLKDAFG+ERRKAKMLNFSIAYGKT VGLS DWKV+V
Sbjct: 843 AVEEKKVLLEWHPQPGQDKPPVPLLKDAFGAERRKAKMLNFSIAYGKTAVGLSWDWKVSV 902
Query: 989 EEARQTVDLWYNERKEVRIWQELRKKEADEKSCVRTLLGRARRFPSMKHATRAQRGHIER 1048
EAR T+ LWY +RKEV WQ+ +K A EK V TLLGR+R+FP+M HA Q+GH+ER
Sbjct: 903 REARDTLKLWYRDRKEVSAWQKKQKAFALEKCEVYTLLGRSRQFPNMTHAGPGQKGHVER 962
Query: 1049 AAINTPVQGSAADVAMCAMLEISNNSGLRELGWRLLLQVHDEVILEGPTESAEVAKAIVV 1108
AAIN PVQGSAADVAMCAMLEI N+ L+ELGWRLLLQVHDEVILEGPTESAE AK IVV
Sbjct: 963 AAINAPVQGSAADVAMCAMLEIERNARLKELGWRLLLQVHDEVILEGPTESAEEAKTIVV 1022
Query: 1109 ECMSKPFSGKNILNVDLAVDAKCAQNWYSAK 1133
ECMSKPF G NIL VDLAVDAK A++WY+AK
Sbjct: 1023 ECMSKPFYGTNILKVDLAVDAKYAKSWYAAK 1033
BLAST of MC10g1035 vs. ExPASy Swiss-Prot
Match:
Q6Z4T3 (DNA polymerase I B, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g0175600 PE=3 SV=1)
HSP 1 Score: 1057.0 bits (2732), Expect = 1.5e-307
Identity = 549/836 (65.67%), Postives = 648/836 (77.51%), Query Frame = 0
Query: 303 EEFSEITINGTGTKMMEAQANDHKPDIKERLNSVYDSVLVVDSVSAAKEVVSMLTTKYKN 362
+E IT T +A + + ++ L ++YD VLVVD+V +A+ VV +LTTKYK
Sbjct: 217 KEHKRITRKVTLNIPDKASLSTESKNARKLLATIYDKVLVVDNVESARSVVKLLTTKYKG 276
Query: 363 LVHACDTEVAKIDVKQETPVDHGEIICFSIYSGPN---ANFGSGKSCIWVDVLDGGGKEI 422
+HACDTEVA IDVK+ETPV HGE+ICFSIYSG + A+FG+GK+CIWVDVLD GG+++
Sbjct: 277 FIHACDTEVANIDVKEETPVGHGEVICFSIYSGNSDGEADFGNGKTCIWVDVLD-GGRDV 336
Query: 423 LLQFAPFFEDPLIKKVWHNYSFDNHIIENYGIKVSGFHADTMHMARLWDSSRRANGGYSL 482
L++FAPFFEDP IKKVWHNYSFD+H+IEN GIKV+GFHADTMH+ARLWDSSRRA+GGYSL
Sbjct: 337 LMEFAPFFEDPSIKKVWHNYSFDSHVIENCGIKVAGFHADTMHLARLWDSSRRADGGYSL 396
Query: 483 EALSGDVKVMSGAKLGHEKELIGKVSMKTIFGRKKMKKDGYEGKLTVIPPVEELQREERR 542
E L+ D ++M+ K GKVSMKTIFGRK ++K+G EGK I PV++LQRE+R
Sbjct: 397 EGLTNDHRIMNAVLKDIHK--TGKVSMKTIFGRKNVRKNGSEGKTISIEPVKKLQREDRE 456
Query: 543 PWVSYSALDSICTLKLYESLKNKLSNMPWERDGEMIPDKTMFNFYEEYWQPFGELLVKME 602
W+ YS+LDS+ TLKLYESLKNKL W DG P TM++FYEEYW+PFG LLVKME
Sbjct: 457 LWICYSSLDSMSTLKLYESLKNKLEAKEWIFDG--CPRGTMYDFYEEYWRPFGALLVKME 516
Query: 603 TEGMLVDRAYLAEIEKLAKAEQEVAGNRFRNWASKYCPDAKYMNVGSDAQVRQLLFGGTL 662
TEGM VDRAYL+EIEK A E+++A ++FR WASK+CPDAKYMNV SD Q+RQL FGG
Sbjct: 517 TEGMFVDRAYLSEIEKTAVVERKLAADKFRKWASKHCPDAKYMNVNSDNQIRQLFFGGIK 576
Query: 663 NSKNPDESLPAERTFKVPNSENVIEEGKKTPGKFRNITLQSILKD-KVLSTE--MYTASG 722
N P E+ P + FKVPN E++ EGKK P K R I L +I++D K+ +TE T +G
Sbjct: 577 NRNKPGETWPQSKAFKVPNDESIATEGKKIP-KSRTIKLFTIVEDLKLFTTEGKKTTKTG 636
Query: 723 WPSVSGDALKILAGKVSAEFDDFTDAHDDLQSDNEVDNDSETMPHGENKKPVIHESANMS 782
W V GD L LAGK+ D DD Q +E D S +P + + S
Sbjct: 637 WLKVRGDVLWSLAGKIPT--DHIYKIDDDGQEFDE-DGSSVELPE--------QDIEDTS 696
Query: 783 DYGTAFEAFASKEEGREACHAIAALCEVCSIDSLISNFILPLQGSNISGKNGRVHCSLNI 842
YGTA+EAF ++GREACHAIAALCEV SID LIS FI+PLQG +IS K GR+HCSLNI
Sbjct: 697 PYGTAYEAFGGGKKGREACHAIAALCEVFSIDKLISGFIVPLQGDHISCKEGRIHCSLNI 756
Query: 843 NTETGRLSARRPNLQNQPALEKDRYKIRQAFIAAPGNSLIVADYGQLELRILAHLANCKS 902
NTETGRLSAR P+LQNQPALEKDRYKIRQAF+AAPGN+LIVADYGQLELRILAHL NCKS
Sbjct: 757 NTETGRLSARTPSLQNQPALEKDRYKIRQAFVAAPGNTLIVADYGQLELRILAHLTNCKS 816
Query: 903 MLEAFKAGGDFHSRTAMNMYPHIRNAVEKGSVLLEWDPQPGEDKPPVPLLKDAFGSERRK 962
MLEAFKAGGDFHSRTAMNMY H+R+AVE+ VLLEW PQPG+DKPPVPLLKDAFG+ERRK
Sbjct: 817 MLEAFKAGGDFHSRTAMNMYQHVRDAVEEKKVLLEWHPQPGQDKPPVPLLKDAFGAERRK 876
Query: 963 AKMLNFSIAYGKTPVGLSKDWKVTVEEARQTVDLWYNERKEVRIWQELRKKEADEKSCVR 1022
AKMLNFSIAYGKT VGLS+DW V V EAR T+ LW+ +RKE+ WQ+ +K A EK V
Sbjct: 877 AKMLNFSIAYGKTAVGLSQDWNVEVREARDTLKLWHRDRKEISAWQKKQKALAFEKCEVY 936
Query: 1023 TLLGRARRFPSMKHATRAQRGHIERAAINTPVQGSAADVAMCAMLEISNNSGLRELGWRL 1082
TLLGR+R+FP+M HA Q+ H+ERAAIN PVQGSAADVAMCAMLEI N+ L+ELGWRL
Sbjct: 937 TLLGRSRQFPNMTHAGPGQKSHVERAAINAPVQGSAADVAMCAMLEIERNARLKELGWRL 996
Query: 1083 LLQVHDEVILEGPTESAEVAKAIVVECMSKPFSGKNILNVDLAVDAKCAQNWYSAK 1133
LLQVHDEVILEGPTESAE AKAIVVECMSKPF G NIL VDLAVDAK A++WY+AK
Sbjct: 997 LLQVHDEVILEGPTESAEEAKAIVVECMSKPFYGTNILKVDLAVDAKYAKSWYAAK 1035
BLAST of MC10g1035 vs. ExPASy Swiss-Prot
Match:
Q9CDS1 (DNA polymerase I OS=Lactococcus lactis subsp. lactis (strain IL1403) OX=272623 GN=polA PE=3 SV=1)
HSP 1 Score: 179.9 bits (455), Expect = 1.6e-43
Identity = 103/307 (33.55%), Postives = 168/307 (54.72%), Query Frame = 0
Query: 827 NGRVHCS-LNINTETGRLSARRPNLQNQPALEKDRYKIRQAFIAAPGNSLIVADYGQLEL 886
+G++H + T+TGRLS+ PNLQN P ++ KIR+AF+ + + L+ +DY Q+EL
Sbjct: 600 DGKIHTRYVQDLTQTGRLSSVDPNLQNIPVRLEEGRKIRKAFVPSKDSLLLSSDYSQIEL 659
Query: 887 RILAHLANCKSMLEAFKAGGDFHSRTAMNMYPHIRNAVEKGSVLLEWDPQPGEDKPPVPL 946
R+LAH++ + +++AFK G D H+ TAM ++ +EK
Sbjct: 660 RVLAHISGDEHLIDAFKHGADIHTSTAMRVF-----GIEKA------------------- 719
Query: 947 LKDAFGSERRKAKMLNFSIAYGKTPVGLSKDWKVTVEEARQTVDLWYNERKEVRIWQELR 1006
+D ++RR AK +NF + YG + GL+++ +T ++A+ ++ ++ ++ + E
Sbjct: 720 -EDVTANDRRNAKAVNFGVVYGISDFGLARNLGITRKDAKNYIETYFERYPGIKTYMENI 779
Query: 1007 KKEADEKSCVRTLLGRARRFPSMKHATRAQRGHIERAAINTPVQGSAADVAMCAMLEISN 1066
+EA +K V T+ R R+ P + RG ER AIN+P+QGSAAD+ AM+ +
Sbjct: 780 VREARDKGFVETMSHRRRKIPDINARNFNVRGFAERTAINSPIQGSAADILKIAMINLDK 839
Query: 1067 NSGLRELGWRLLLQVHDEVILEGPTESAEVAKAIVVECMSKPFSGKNILNVDLAVDAKCA 1126
R+ +LLLQVHDE+IL+ P E + K +V + M + L V L VD
Sbjct: 840 ALSARDFKSKLLLQVHDEIILDVPLEELDEIKVLVKQTMEEAIE----LAVPLKVDENTG 877
Query: 1127 QNWYSAK 1133
+ WY AK
Sbjct: 900 KTWYEAK 877
BLAST of MC10g1035 vs. NCBI nr
Match:
XP_022150985.1 (DNA polymerase I A, chloroplastic/mitochondrial [Momordica charantia])
HSP 1 Score: 2247 bits (5822), Expect = 0.0
Identity = 1132/1132 (100.00%), Postives = 1132/1132 (100.00%), Query Frame = 0
Query: 1 MMTLGVSTTQASSLRTSWPSYFFLWRSNSVSNSSISICASSKALYRSEFSSMKSGDGASP 60
MMTLGVSTTQASSLRTSWPSYFFLWRSNSVSNSSISICASSKALYRSEFSSMKSGDGASP
Sbjct: 1 MMTLGVSTTQASSLRTSWPSYFFLWRSNSVSNSSISICASSKALYRSEFSSMKSGDGASP 60
Query: 61 TLNMFHASIQCRKSSFLSTNSLVETRQYDNERAFLSDVNAWSKSTMQIKQEKHFRFMESG 120
TLNMFHASIQCRKSSFLSTNSLVETRQYDNERAFLSDVNAWSKSTMQIKQEKHFRFMESG
Sbjct: 61 TLNMFHASIQCRKSSFLSTNSLVETRQYDNERAFLSDVNAWSKSTMQIKQEKHFRFMESG 120
Query: 121 ILTKSDEEKLRKMENLVGYGTAHSYNRPQYSKVQCFNANKDSDCINPETNRLSDGFRKQE 180
ILTKSDEEKLRKMENLVGYGTAHSYNRPQYSKVQCFNANKDSDCINPETNRLSDGFRKQE
Sbjct: 121 ILTKSDEEKLRKMENLVGYGTAHSYNRPQYSKVQCFNANKDSDCINPETNRLSDGFRKQE 180
Query: 181 PMNFERSVSAATIDRKTDSDRGPSIKTFKVSRGECNGDIDSFSGGRTMNKPENNDLHNQL 240
PMNFERSVSAATIDRKTDSDRGPSIKTFKVSRGECNGDIDSFSGGRTMNKPENNDLHNQL
Sbjct: 181 PMNFERSVSAATIDRKTDSDRGPSIKTFKVSRGECNGDIDSFSGGRTMNKPENNDLHNQL 240
Query: 241 VPMRSNKRYTISQNGKGSISHHAPNVSPNGRKQNISTGKVNNVPKSLKFIEASNEIKRGV 300
VPMRSNKRYTISQNGKGSISHHAPNVSPNGRKQNISTGKVNNVPKSLKFIEASNEIKRGV
Sbjct: 241 VPMRSNKRYTISQNGKGSISHHAPNVSPNGRKQNISTGKVNNVPKSLKFIEASNEIKRGV 300
Query: 301 DVEEFSEITINGTGTKMMEAQANDHKPDIKERLNSVYDSVLVVDSVSAAKEVVSMLTTKY 360
DVEEFSEITINGTGTKMMEAQANDHKPDIKERLNSVYDSVLVVDSVSAAKEVVSMLTTKY
Sbjct: 301 DVEEFSEITINGTGTKMMEAQANDHKPDIKERLNSVYDSVLVVDSVSAAKEVVSMLTTKY 360
Query: 361 KNLVHACDTEVAKIDVKQETPVDHGEIICFSIYSGPNANFGSGKSCIWVDVLDGGGKEIL 420
KNLVHACDTEVAKIDVKQETPVDHGEIICFSIYSGPNANFGSGKSCIWVDVLDGGGKEIL
Sbjct: 361 KNLVHACDTEVAKIDVKQETPVDHGEIICFSIYSGPNANFGSGKSCIWVDVLDGGGKEIL 420
Query: 421 LQFAPFFEDPLIKKVWHNYSFDNHIIENYGIKVSGFHADTMHMARLWDSSRRANGGYSLE 480
LQFAPFFEDPLIKKVWHNYSFDNHIIENYGIKVSGFHADTMHMARLWDSSRRANGGYSLE
Sbjct: 421 LQFAPFFEDPLIKKVWHNYSFDNHIIENYGIKVSGFHADTMHMARLWDSSRRANGGYSLE 480
Query: 481 ALSGDVKVMSGAKLGHEKELIGKVSMKTIFGRKKMKKDGYEGKLTVIPPVEELQREERRP 540
ALSGDVKVMSGAKLGHEKELIGKVSMKTIFGRKKMKKDGYEGKLTVIPPVEELQREERRP
Sbjct: 481 ALSGDVKVMSGAKLGHEKELIGKVSMKTIFGRKKMKKDGYEGKLTVIPPVEELQREERRP 540
Query: 541 WVSYSALDSICTLKLYESLKNKLSNMPWERDGEMIPDKTMFNFYEEYWQPFGELLVKMET 600
WVSYSALDSICTLKLYESLKNKLSNMPWERDGEMIPDKTMFNFYEEYWQPFGELLVKMET
Sbjct: 541 WVSYSALDSICTLKLYESLKNKLSNMPWERDGEMIPDKTMFNFYEEYWQPFGELLVKMET 600
Query: 601 EGMLVDRAYLAEIEKLAKAEQEVAGNRFRNWASKYCPDAKYMNVGSDAQVRQLLFGGTLN 660
EGMLVDRAYLAEIEKLAKAEQEVAGNRFRNWASKYCPDAKYMNVGSDAQVRQLLFGGTLN
Sbjct: 601 EGMLVDRAYLAEIEKLAKAEQEVAGNRFRNWASKYCPDAKYMNVGSDAQVRQLLFGGTLN 660
Query: 661 SKNPDESLPAERTFKVPNSENVIEEGKKTPGKFRNITLQSILKDKVLSTEMYTASGWPSV 720
SKNPDESLPAERTFKVPNSENVIEEGKKTPGKFRNITLQSILKDKVLSTEMYTASGWPSV
Sbjct: 661 SKNPDESLPAERTFKVPNSENVIEEGKKTPGKFRNITLQSILKDKVLSTEMYTASGWPSV 720
Query: 721 SGDALKILAGKVSAEFDDFTDAHDDLQSDNEVDNDSETMPHGENKKPVIHESANMSDYGT 780
SGDALKILAGKVSAEFDDFTDAHDDLQSDNEVDNDSETMPHGENKKPVIHESANMSDYGT
Sbjct: 721 SGDALKILAGKVSAEFDDFTDAHDDLQSDNEVDNDSETMPHGENKKPVIHESANMSDYGT 780
Query: 781 AFEAFASKEEGREACHAIAALCEVCSIDSLISNFILPLQGSNISGKNGRVHCSLNINTET 840
AFEAFASKEEGREACHAIAALCEVCSIDSLISNFILPLQGSNISGKNGRVHCSLNINTET
Sbjct: 781 AFEAFASKEEGREACHAIAALCEVCSIDSLISNFILPLQGSNISGKNGRVHCSLNINTET 840
Query: 841 GRLSARRPNLQNQPALEKDRYKIRQAFIAAPGNSLIVADYGQLELRILAHLANCKSMLEA 900
GRLSARRPNLQNQPALEKDRYKIRQAFIAAPGNSLIVADYGQLELRILAHLANCKSMLEA
Sbjct: 841 GRLSARRPNLQNQPALEKDRYKIRQAFIAAPGNSLIVADYGQLELRILAHLANCKSMLEA 900
Query: 901 FKAGGDFHSRTAMNMYPHIRNAVEKGSVLLEWDPQPGEDKPPVPLLKDAFGSERRKAKML 960
FKAGGDFHSRTAMNMYPHIRNAVEKGSVLLEWDPQPGEDKPPVPLLKDAFGSERRKAKML
Sbjct: 901 FKAGGDFHSRTAMNMYPHIRNAVEKGSVLLEWDPQPGEDKPPVPLLKDAFGSERRKAKML 960
Query: 961 NFSIAYGKTPVGLSKDWKVTVEEARQTVDLWYNERKEVRIWQELRKKEADEKSCVRTLLG 1020
NFSIAYGKTPVGLSKDWKVTVEEARQTVDLWYNERKEVRIWQELRKKEADEKSCVRTLLG
Sbjct: 961 NFSIAYGKTPVGLSKDWKVTVEEARQTVDLWYNERKEVRIWQELRKKEADEKSCVRTLLG 1020
Query: 1021 RARRFPSMKHATRAQRGHIERAAINTPVQGSAADVAMCAMLEISNNSGLRELGWRLLLQV 1080
RARRFPSMKHATRAQRGHIERAAINTPVQGSAADVAMCAMLEISNNSGLRELGWRLLLQV
Sbjct: 1021 RARRFPSMKHATRAQRGHIERAAINTPVQGSAADVAMCAMLEISNNSGLRELGWRLLLQV 1080
Query: 1081 HDEVILEGPTESAEVAKAIVVECMSKPFSGKNILNVDLAVDAKCAQNWYSAK 1132
HDEVILEGPTESAEVAKAIVVECMSKPFSGKNILNVDLAVDAKCAQNWYSAK
Sbjct: 1081 HDEVILEGPTESAEVAKAIVVECMSKPFSGKNILNVDLAVDAKCAQNWYSAK 1132
BLAST of MC10g1035 vs. NCBI nr
Match:
KAG7015499.1 (DNA polymerase I A, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1870 bits (4845), Expect = 0.0
Identity = 948/1140 (83.16%), Postives = 1025/1140 (89.91%), Query Frame = 0
Query: 1 MMTLGVSTTQASSLRTSWPSYFFLWRSNSVSNSSISICASSKALYRSEFSSMKSGDGASP 60
M+TLG ST+QASSL T WPSYFFLWRSNSVSNSSISICASSK LYR+EFSS+KS ASP
Sbjct: 65 MITLGASTSQASSLITQWPSYFFLWRSNSVSNSSISICASSKRLYRAEFSSLKSVRSASP 124
Query: 61 TLNMFHASIQCRKSSFLSTNSLVETRQYDNERAFLSDVNAWSKSTMQIKQEKHFRFMESG 120
+NMFHAS+QCR+SSFL TNS ETRQ+D ERAFLSD+N WSKST Q+KQEK FRF E+
Sbjct: 125 NVNMFHASLQCRQSSFLCTNSFFETRQHDKERAFLSDINDWSKSTRQLKQEKLFRFSETE 184
Query: 121 ILTKSDEEKLRKMENLVGYGTAHSYNR--PQYSKVQ------CFNANKDSDCINPETNRL 180
ILTK+DEEKLRK ENL+GYGT H YN P YSKVQ C NA+ DS+CINP TN L
Sbjct: 185 ILTKNDEEKLRKKENLIGYGTLHCYNSLCPPYSKVQTNLGSNCSNASNDSNCINPPTNML 244
Query: 181 SDGFRKQEPMNFERSVSAATIDRKTDSDRGPSIKTFKVSRGECNGDIDSFSGGRTMNKPE 240
SD F KQEP+NFER+ + ATI R SDR P ++T K SRGECNGD +S+SG R M+KP
Sbjct: 245 SDEFGKQEPINFERTENGATIYRMI-SDRVPLLETVKFSRGECNGDTNSYSGERPMSKPA 304
Query: 241 NNDLHNQLVPMRSNKRYTISQNGKGSISHHAPNVSPNGRKQNISTGKVNNVPKSLKFIEA 300
NN LH+Q+VPM+SNK+Y++SQNGKGSI H PNVSPNGR +NIS GKVN+V K+LK EA
Sbjct: 305 NNVLHSQVVPMQSNKKYSVSQNGKGSIMRHVPNVSPNGRNRNISLGKVNSVLKTLKCTEA 364
Query: 301 SNEIKRGVDVEEFSEITINGTGTKMMEAQANDHKPDIKERLNSVYDSVLVVDSVSAAKEV 360
+N I +GV VEEFS++ +NG+GTKMME A HKPDIKERLN VY+SVLVVD VSAAKEV
Sbjct: 365 ANGINKGVAVEEFSKVIVNGSGTKMMEVLATAHKPDIKERLNGVYESVLVVDGVSAAKEV 424
Query: 361 VSMLTTKYKNLVHACDTEVAKIDVKQETPVDHGEIICFSIYSGPNANFGSGKSCIWVDVL 420
VSMLTTKYKNLVHACDTEVAKIDVKQETPVDHGEIICFSIYSGP A+FG+GKSCIWVDVL
Sbjct: 425 VSMLTTKYKNLVHACDTEVAKIDVKQETPVDHGEIICFSIYSGPKADFGNGKSCIWVDVL 484
Query: 421 DGGGKEILLQFAPFFEDPLIKKVWHNYSFDNHIIENYGIKVSGFHADTMHMARLWDSSRR 480
DGGGKEILLQFAPFFEDPLI+KVWHNYSFDNHIIENYGIK+SGFHADTMHMARLWDSSRR
Sbjct: 485 DGGGKEILLQFAPFFEDPLIRKVWHNYSFDNHIIENYGIKISGFHADTMHMARLWDSSRR 544
Query: 481 ANGGYSLEALSGDVKVMSGAKLGHEKELIGKVSMKTIFGRKKMKKDGYEGKLTVIPPVEE 540
NGGYSLEALS D KVMSGAKL EKELIGKVSMKTIFGRKKMKKDG EGKL VIPPVEE
Sbjct: 545 MNGGYSLEALSCDTKVMSGAKLDQEKELIGKVSMKTIFGRKKMKKDGSEGKLIVIPPVEE 604
Query: 541 LQREERRPWVSYSALDSICTLKLYESLKNKLSNMPWERDGEMIPDKTMFNFYEEYWQPFG 600
LQREER+PWVSYSALDSICTLKLYESLK KLS+MPWERDGE IPDKTMFNFYE+YW+PFG
Sbjct: 605 LQREERKPWVSYSALDSICTLKLYESLKKKLSDMPWERDGERIPDKTMFNFYEDYWKPFG 664
Query: 601 ELLVKMETEGMLVDRAYLAEIEKLAKAEQEVAGNRFRNWASKYCPDAKYMNVGSDAQVRQ 660
E+LV+METEGMLVDR YLAEIEKLAKAEQEVA NRFRNWAS+YCPDAKYMNVGSDAQVRQ
Sbjct: 665 EVLVRMETEGMLVDRPYLAEIEKLAKAEQEVAANRFRNWASEYCPDAKYMNVGSDAQVRQ 724
Query: 661 LLFGGTLNSKNPDESLPAERTFKVPNSENVIEEGKKTPGKFRNITLQSILKDKVLSTEMY 720
LLFGGT NSKNP+ESLP ERTFK+PNSE V EEGKKTP KFRNITL+ D+ LSTE+Y
Sbjct: 725 LLFGGTCNSKNPEESLPTERTFKIPNSEKVTEEGKKTPSKFRNITLRRF-SDEALSTELY 784
Query: 721 TASGWPSVSGDALKILAGKVSAEFDDFTDAHDDLQSDNEVDNDSETMPHGENKKPVIHES 780
TA+GWPSVSGDALKILAGKVSAEFDDFTD D QSD EV ND ETMPH EN++ ++HE
Sbjct: 785 TATGWPSVSGDALKILAGKVSAEFDDFTD---DPQSDTEVVNDFETMPHEENRRRIVHEC 844
Query: 781 ANMSDYGTAFEAFASKEEGREACHAIAALCEVCSIDSLISNFILPLQGSNISGKNGRVHC 840
ANMSDYGTA +AF KEEG EACHAIAALCE+CSIDSLISNFILPLQGSNISGKNGRVHC
Sbjct: 845 ANMSDYGTALKAFKLKEEGMEACHAIAALCEICSIDSLISNFILPLQGSNISGKNGRVHC 904
Query: 841 SLNINTETGRLSARRPNLQNQPALEKDRYKIRQAFIAAPGNSLIVADYGQLELRILAHLA 900
SLNINTETGRLSARRP+LQNQPALEKDRYKIRQAFIA+PGNSLIVADYGQLELRILAHLA
Sbjct: 905 SLNINTETGRLSARRPSLQNQPALEKDRYKIRQAFIASPGNSLIVADYGQLELRILAHLA 964
Query: 901 NCKSMLEAFKAGGDFHSRTAMNMYPHIRNAVEKGSVLLEWDPQPGEDKPPVPLLKDAFGS 960
NC+SML+AFKAGGDFHSRTAMNMYPHIR AVE+GSVLLEWDPQPGEDKPPVPLLKDAF S
Sbjct: 965 NCQSMLDAFKAGGDFHSRTAMNMYPHIRKAVEEGSVLLEWDPQPGEDKPPVPLLKDAFAS 1024
Query: 961 ERRKAKMLNFSIAYGKTPVGLSKDWKVTVEEARQTVDLWYNERKEVRIWQELRKKEADEK 1020
ERRKAKMLNFSIAYGKTP+GLSKDWKVTVEEA +TVDLWYNER EVR WQELR++EA++K
Sbjct: 1025 ERRKAKMLNFSIAYGKTPIGLSKDWKVTVEEASKTVDLWYNERTEVRRWQELRREEAEKK 1084
Query: 1021 SCVRTLLGRARRFPSMKHATRAQRGHIERAAINTPVQGSAADVAMCAMLEISNNSGLREL 1080
SCVRTLLGRAR+FPSMK TRAQ+GHIERAAINTPVQGSAADVAMCAMLEIS NS LREL
Sbjct: 1085 SCVRTLLGRARQFPSMKQVTRAQKGHIERAAINTPVQGSAADVAMCAMLEISKNSRLREL 1144
Query: 1081 GWRLLLQVHDEVILEGPTESAEVAKAIVVECMSKPFSGKNILNVDLAVDAKCAQNWYSAK 1132
GWRLLLQVHDEVILEGPTESAEVAKAIVV+CMSKPF+GKNIL VDLAVDAKCAQNWYSAK
Sbjct: 1145 GWRLLLQVHDEVILEGPTESAEVAKAIVVDCMSKPFNGKNILKVDLAVDAKCAQNWYSAK 1199
BLAST of MC10g1035 vs. NCBI nr
Match:
KAG7031413.1 (DNA polymerase I A, chloroplastic/mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1866 bits (4834), Expect = 0.0
Identity = 945/1133 (83.41%), Postives = 1022/1133 (90.20%), Query Frame = 0
Query: 1 MMTLGVSTTQASSLRTSWPSYFFLWRSNSVSNSSISICASSKALYRSEFSSMKSGDGASP 60
MMTLGVS++QASSLR WP+YFFLWRSNSVS+SSIS CASSKAL R+EF +KS G S
Sbjct: 1 MMTLGVSSSQASSLRAQWPAYFFLWRSNSVSSSSISFCASSKALCRAEFGPLKSVGGVSS 60
Query: 61 TLNMFHASIQCRKSSFLSTNSLVETRQYDNERAFLSDVNAWSKSTMQIKQEKHFRFMESG 120
+ FHAS QCR+SSFLSTNS VETRQYDNERAFLSDV AWSKSTM++K+EKH RFME+
Sbjct: 61 NMKTFHASCQCRQSSFLSTNSFVETRQYDNERAFLSDVKAWSKSTMRLKEEKHLRFMETE 120
Query: 121 ILTKSDEEKLRKMENLVGYGTAHSYNRPQY-SKVQCFNANKDSDCINPETNRLSDGFRKQ 180
ILT +DEEKLR E+L+GYG++HS + SK+ NANKDSDCINP TN LSDGFRKQ
Sbjct: 121 ILTTNDEEKLRNEEHLIGYGSSHSKIQSNLGSKLS--NANKDSDCINPSTNMLSDGFRKQ 180
Query: 181 EPMNFERSVSAATIDRKTDSDRGPSIKTFKVSRGECNGDIDSFSGGRTMNKPENNDLHNQ 240
PM+FE+ + TI+ SDR S+ T KVS CNG+I S+SG +TM KP NNDLHNQ
Sbjct: 181 GPMSFEQLQNVETIEEMMISDRTLSLDTIKVSSDRCNGNISSYSGEQTMTKPANNDLHNQ 240
Query: 241 LVPMRSNKRYTISQNGKGSISHHAPNVSPNGRKQNISTGKVNNVPKSLKFIEASNEIKRG 300
++PM+SNK YT SQNGKGSI H +PNVSPNGRKQ+I GK+++VPK+LK EA+N +KRG
Sbjct: 241 VIPMQSNKNYTFSQNGKGSIMHRSPNVSPNGRKQSIPLGKMDSVPKTLKLTEAANGLKRG 300
Query: 301 VDVEEFSEITINGTGTKMMEAQANDHKPDIKERLNSVYDSVLVVDSVSAAKEVVSMLTTK 360
VEEFS++TING GTK+ EA A +HKPDIKERLN VYDSV VVDS+ AA+EVVSMLT K
Sbjct: 301 AAVEEFSKMTINGGGTKITEAPATNHKPDIKERLNGVYDSVHVVDSIRAAREVVSMLTMK 360
Query: 361 YKNLVHACDTEVAKIDVKQETPVDHGEIICFSIYSGPNANFGSGKSCIWVDVLDGGGKEI 420
Y+NLVHACDTEVAKIDVKQETPVDHGEIICFSIYSGP A+FG+GKSCIWVDVLDGGGKEI
Sbjct: 361 YRNLVHACDTEVAKIDVKQETPVDHGEIICFSIYSGPTADFGNGKSCIWVDVLDGGGKEI 420
Query: 421 LLQFAPFFEDPLIKKVWHNYSFDNHIIENYGIKVSGFHADTMHMARLWDSSRRANGGYSL 480
LLQFAPFFEDP I+KVWHNYSFDNHIIENYGIKVSGFHADTMHMARLWDSSRR NGGYSL
Sbjct: 421 LLQFAPFFEDPSIRKVWHNYSFDNHIIENYGIKVSGFHADTMHMARLWDSSRRINGGYSL 480
Query: 481 EALSGDVKVMSGAKLGHEKELIGKVSMKTIFGRKKMKKDGYEGKLTVIPPVEELQREERR 540
EALSGD KVMSGAKLG EKELIGK+SMKTIFGRKKMKKDG EGK+ VIPPVEELQREE++
Sbjct: 481 EALSGDTKVMSGAKLGQEKELIGKISMKTIFGRKKMKKDGSEGKIIVIPPVEELQREEKK 540
Query: 541 PWVSYSALDSICTLKLYESLKNKLSNMPWERDGEMIPDKTMFNFYEEYWQPFGELLVKME 600
WVSYSALDSICTLKLYESLKNKLS+MPWER+GEMIP+KTMFNFYEEYWQPFGELLVKME
Sbjct: 541 LWVSYSALDSICTLKLYESLKNKLSDMPWERNGEMIPNKTMFNFYEEYWQPFGELLVKME 600
Query: 601 TEGMLVDRAYLAEIEKLAKAEQEVAGNRFRNWASKYCPDAKYMNVGSDAQVRQLLFGGTL 660
TEGMLVDR YLA+IEKLA AEQ+VA NRFRNWASKYCPDA++MNVGSDAQ+RQLLFGGT
Sbjct: 601 TEGMLVDRPYLAKIEKLAIAEQQVAANRFRNWASKYCPDARHMNVGSDAQLRQLLFGGTS 660
Query: 661 NSKNPDESLPAERTFKVPNSENVIEEGKKTPGKFRNITLQSILKDKVLSTEMYTASGWPS 720
NSKNPDESLP ERTFKVPN+ENVIEEGKKTP KFRNITL+ I + LSTEMYTA+GWPS
Sbjct: 661 NSKNPDESLPTERTFKVPNTENVIEEGKKTPSKFRNITLKRISVED-LSTEMYTATGWPS 720
Query: 721 VSGDALKILAGKVSAEFDDFTDAHDDLQSDNEVDNDSETMPHGENKKPVIHESANMSDYG 780
VSGDALK+LAGKVSAE+D FTD DLQSDNE +DSET H ENKK +IHESANMSDYG
Sbjct: 721 VSGDALKVLAGKVSAEYDYFTD---DLQSDNEFGDDSETTSHEENKKHIIHESANMSDYG 780
Query: 781 TAFEAFASKEEGREACHAIAALCEVCSIDSLISNFILPLQGSNISGKNGRVHCSLNINTE 840
A +AF S E+GREACHAIAALCEVCSIDSLISNFILPLQGSNISGKNGR+HCSLNINTE
Sbjct: 781 AALKAFGSSEKGREACHAIAALCEVCSIDSLISNFILPLQGSNISGKNGRIHCSLNINTE 840
Query: 841 TGRLSARRPNLQNQPALEKDRYKIRQAFIAAPGNSLIVADYGQLELRILAHLANCKSMLE 900
TGRLSARRPNLQNQPALEKDRYKIRQAFIAAPGNSLIVADYGQLELRILAHLANCKSMLE
Sbjct: 841 TGRLSARRPNLQNQPALEKDRYKIRQAFIAAPGNSLIVADYGQLELRILAHLANCKSMLE 900
Query: 901 AFKAGGDFHSRTAMNMYPHIRNAVEKGSVLLEWDPQPGEDKPPVPLLKDAFGSERRKAKM 960
AFKAGGDFHSRTAMNMYPHIR AVE GSVLLEWDPQPGEDKPPVPLLKDAFGSERRKAKM
Sbjct: 901 AFKAGGDFHSRTAMNMYPHIRKAVEDGSVLLEWDPQPGEDKPPVPLLKDAFGSERRKAKM 960
Query: 961 LNFSIAYGKTPVGLSKDWKVTVEEARQTVDLWYNERKEVRIWQELRKKEADEKSCVRTLL 1020
LNFSIAYGKTPVGLSKDWKVTVEEA+QTVDLWYNERKEVR WQ LRK+EA+EKSCVRTLL
Sbjct: 961 LNFSIAYGKTPVGLSKDWKVTVEEAKQTVDLWYNERKEVRTWQNLRKREAEEKSCVRTLL 1020
Query: 1021 GRARRFPSMKHATRAQRGHIERAAINTPVQGSAADVAMCAMLEISNNSGLRELGWRLLLQ 1080
GRARRFPSM HATRA +GHIERAAINTPVQGSAADVAMCAMLEISNNS LRELGWRLLLQ
Sbjct: 1021 GRARRFPSMTHATRAHKGHIERAAINTPVQGSAADVAMCAMLEISNNSRLRELGWRLLLQ 1080
Query: 1081 VHDEVILEGPTESAEVAKAIVVECMSKPFSGKNILNVDLAVDAKCAQNWYSAK 1132
VHDEVILEGPTESAEVAKAIVVECMSKPF+GKNILNVDLAVDAKCA+NWYSAK
Sbjct: 1081 VHDEVILEGPTESAEVAKAIVVECMSKPFNGKNILNVDLAVDAKCARNWYSAK 1127
BLAST of MC10g1035 vs. NCBI nr
Match:
XP_023536872.1 (DNA polymerase I B, chloroplastic/mitochondrial-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1866 bits (4833), Expect = 0.0
Identity = 944/1133 (83.32%), Postives = 1023/1133 (90.29%), Query Frame = 0
Query: 1 MMTLGVSTTQASSLRTSWPSYFFLWRSNSVSNSSISICASSKALYRSEFSSMKSGDGASP 60
MMTLGVS++QASSLR WP+YFFLWRSNSVS+SSIS CASSKAL R+EF +KS G S
Sbjct: 1 MMTLGVSSSQASSLRAQWPAYFFLWRSNSVSSSSISFCASSKALCRAEFGPLKSVGGVSS 60
Query: 61 TLNMFHASIQCRKSSFLSTNSLVETRQYDNERAFLSDVNAWSKSTMQIKQEKHFRFMESG 120
+ FHAS QCR+SSFLSTNS VETRQYDNERAFLSDV AWSKSTM++K+EKH RFME+
Sbjct: 61 NMKTFHASCQCRQSSFLSTNSFVETRQYDNERAFLSDVKAWSKSTMRLKEEKHLRFMETE 120
Query: 121 ILTKSDEEKLRKMENLVGYGTAHSYNRPQY-SKVQCFNANKDSDCINPETNRLSDGFRKQ 180
ILT +DEEKLR E+L+GYGT+HS + SK+ NANKDSDCINP TN LSDGFRKQ
Sbjct: 121 ILTTNDEEKLRNEEHLIGYGTSHSKIQSNLGSKLS--NANKDSDCINPSTNMLSDGFRKQ 180
Query: 181 EPMNFERSVSAATIDRKTDSDRGPSIKTFKVSRGECNGDIDSFSGGRTMNKPENNDLHNQ 240
PM+FE+ + TI+ SDR S+ T KVS CNG+I S+SG +TM KP NNDLHNQ
Sbjct: 181 GPMSFEQIQNVETIEGMIISDRTLSLDTIKVSSDRCNGNISSYSGEQTMTKPANNDLHNQ 240
Query: 241 LVPMRSNKRYTISQNGKGSISHHAPNVSPNGRKQNISTGKVNNVPKSLKFIEASNEIKRG 300
++PM+SNK YT SQNG GSI H +PNVSPNGRKQ+I GK+++VPK+LK EA+N +KRG
Sbjct: 241 VIPMQSNKNYTFSQNGNGSIMHRSPNVSPNGRKQSIPLGKMDSVPKTLKLTEAANGLKRG 300
Query: 301 VDVEEFSEITINGTGTKMMEAQANDHKPDIKERLNSVYDSVLVVDSVSAAKEVVSMLTTK 360
VEEFS++TING GTK+ EA A +HKPDIKERLN VYDSVLVVDS+ AA+E+VSMLT K
Sbjct: 301 AAVEEFSKMTINGGGTKITEAPATNHKPDIKERLNGVYDSVLVVDSIHAAREIVSMLTMK 360
Query: 361 YKNLVHACDTEVAKIDVKQETPVDHGEIICFSIYSGPNANFGSGKSCIWVDVLDGGGKEI 420
Y+NLVHACDTEVAKIDVKQETPVDHGEIICFSIYSGP A+FG+GKSCIWVDVLDGGGKEI
Sbjct: 361 YRNLVHACDTEVAKIDVKQETPVDHGEIICFSIYSGPTADFGNGKSCIWVDVLDGGGKEI 420
Query: 421 LLQFAPFFEDPLIKKVWHNYSFDNHIIENYGIKVSGFHADTMHMARLWDSSRRANGGYSL 480
LLQFAPFFEDP I+KVWHNYSFDNHIIENYGIKVSGFHADTMHMARLWDSSRR NGGYSL
Sbjct: 421 LLQFAPFFEDPSIRKVWHNYSFDNHIIENYGIKVSGFHADTMHMARLWDSSRRINGGYSL 480
Query: 481 EALSGDVKVMSGAKLGHEKELIGKVSMKTIFGRKKMKKDGYEGKLTVIPPVEELQREERR 540
EALSGD KVMSGAKLG EKELIGK+SMKTIFGRKKMKKDG EGK+ VIPPVEELQREE++
Sbjct: 481 EALSGDTKVMSGAKLGQEKELIGKISMKTIFGRKKMKKDGSEGKIIVIPPVEELQREEKK 540
Query: 541 PWVSYSALDSICTLKLYESLKNKLSNMPWERDGEMIPDKTMFNFYEEYWQPFGELLVKME 600
WVSYSALDSICTLKLYESLKNKLS+MPWER+GEMIP+KTMFNFYEEYWQPFGELL+KME
Sbjct: 541 LWVSYSALDSICTLKLYESLKNKLSDMPWERNGEMIPNKTMFNFYEEYWQPFGELLIKME 600
Query: 601 TEGMLVDRAYLAEIEKLAKAEQEVAGNRFRNWASKYCPDAKYMNVGSDAQVRQLLFGGTL 660
TEGMLVDR YLA+IEKLA AEQ+VA NRFRNWASKYCPDA++MNVGSDAQ+RQLLFGGT
Sbjct: 601 TEGMLVDRPYLAKIEKLAIAEQQVAANRFRNWASKYCPDARHMNVGSDAQLRQLLFGGTS 660
Query: 661 NSKNPDESLPAERTFKVPNSENVIEEGKKTPGKFRNITLQSILKDKVLSTEMYTASGWPS 720
NSKNPDESLP ERTFKVPN+ENVIEEGKKTP KFRNITL+ I + LSTEMYTA+GWPS
Sbjct: 661 NSKNPDESLPTERTFKVPNTENVIEEGKKTPSKFRNITLKRISVED-LSTEMYTATGWPS 720
Query: 721 VSGDALKILAGKVSAEFDDFTDAHDDLQSDNEVDNDSETMPHGENKKPVIHESANMSDYG 780
VSGDALK+LAGKVSAE+D FTD DLQSDNE ++SETM H ENKK +IHESANMSDYG
Sbjct: 721 VSGDALKVLAGKVSAEYDYFTD---DLQSDNEFGDNSETMFHEENKKHIIHESANMSDYG 780
Query: 781 TAFEAFASKEEGREACHAIAALCEVCSIDSLISNFILPLQGSNISGKNGRVHCSLNINTE 840
TA +AF S E+GREACHAIAALCEVCSIDSLISNFILPLQGSNISGKNGR+HCSLNINTE
Sbjct: 781 TALKAFGSSEKGREACHAIAALCEVCSIDSLISNFILPLQGSNISGKNGRIHCSLNINTE 840
Query: 841 TGRLSARRPNLQNQPALEKDRYKIRQAFIAAPGNSLIVADYGQLELRILAHLANCKSMLE 900
TGRLSARRPNLQNQPALEKDRYKIRQAFIAAPGNSLIVADYGQLELRILAHLANCKSMLE
Sbjct: 841 TGRLSARRPNLQNQPALEKDRYKIRQAFIAAPGNSLIVADYGQLELRILAHLANCKSMLE 900
Query: 901 AFKAGGDFHSRTAMNMYPHIRNAVEKGSVLLEWDPQPGEDKPPVPLLKDAFGSERRKAKM 960
AFKAGGDFHSRTAMNMYPHIR AVE GSVLLEWDPQPGEDKPPVPLLKDAFGSERRKAKM
Sbjct: 901 AFKAGGDFHSRTAMNMYPHIRKAVEDGSVLLEWDPQPGEDKPPVPLLKDAFGSERRKAKM 960
Query: 961 LNFSIAYGKTPVGLSKDWKVTVEEARQTVDLWYNERKEVRIWQELRKKEADEKSCVRTLL 1020
LNFSIAYGKTPVGLSKDWKVTVEEA+QTVDLWYNERKEVR WQ LRK+EA+EKSCVRTLL
Sbjct: 961 LNFSIAYGKTPVGLSKDWKVTVEEAKQTVDLWYNERKEVRTWQNLRKREAEEKSCVRTLL 1020
Query: 1021 GRARRFPSMKHATRAQRGHIERAAINTPVQGSAADVAMCAMLEISNNSGLRELGWRLLLQ 1080
GRARRFPSM HATRA +GHIERAAINTPVQGSAADVAMCAMLEIS NS LRELGWRLLLQ
Sbjct: 1021 GRARRFPSMTHATRAHKGHIERAAINTPVQGSAADVAMCAMLEISKNSRLRELGWRLLLQ 1080
Query: 1081 VHDEVILEGPTESAEVAKAIVVECMSKPFSGKNILNVDLAVDAKCAQNWYSAK 1132
VHDEVILEGPTESAEVAKAIVVECMSKPF+GKNILNVDLAVDAKCA+NWYSAK
Sbjct: 1081 VHDEVILEGPTESAEVAKAIVVECMSKPFNGKNILNVDLAVDAKCARNWYSAK 1127
BLAST of MC10g1035 vs. NCBI nr
Match:
KAG6600776.1 (DNA polymerase I A, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1862 bits (4823), Expect = 0.0
Identity = 943/1132 (83.30%), Postives = 1020/1132 (90.11%), Query Frame = 0
Query: 2 MTLGVSTTQASSLRTSWPSYFFLWRSNSVSNSSISICASSKALYRSEFSSMKSGDGASPT 61
MTLGVS++QASSLR WP+YFFLWRSNSVS+SSIS CASSKAL R+EF +KS G S
Sbjct: 1 MTLGVSSSQASSLRAQWPAYFFLWRSNSVSSSSISFCASSKALCRAEFGPLKSVGGVSSN 60
Query: 62 LNMFHASIQCRKSSFLSTNSLVETRQYDNERAFLSDVNAWSKSTMQIKQEKHFRFMESGI 121
+ FHAS QCR+SSFLSTNS VETRQYDNERAFLSDV AWSKSTM++K+EKH RFME+ I
Sbjct: 61 MKTFHASCQCRQSSFLSTNSFVETRQYDNERAFLSDVKAWSKSTMRLKEEKHLRFMETEI 120
Query: 122 LTKSDEEKLRKMENLVGYGTAHSYNRPQY-SKVQCFNANKDSDCINPETNRLSDGFRKQE 181
LT +DEEKLR E+L+GYG++HS + SK+ NANKDSDCINP TN LSDGFRKQ
Sbjct: 121 LTTNDEEKLRNEEHLIGYGSSHSKIQSNLGSKLS--NANKDSDCINPSTNMLSDGFRKQG 180
Query: 182 PMNFERSVSAATIDRKTDSDRGPSIKTFKVSRGECNGDIDSFSGGRTMNKPENNDLHNQL 241
PM+FE+ + TI+ SDR S+ T KVS CNG+I S+SG +TM KP +NDLHNQ+
Sbjct: 181 PMSFEQLQNVETIEEMMISDRTLSLDTIKVSSDRCNGNISSYSGEQTMTKPASNDLHNQV 240
Query: 242 VPMRSNKRYTISQNGKGSISHHAPNVSPNGRKQNISTGKVNNVPKSLKFIEASNEIKRGV 301
+PM+SNK YT SQNGKGSI H +PNVSPNGRKQ+I GK+++VPK+LK EA+N +KRG
Sbjct: 241 IPMQSNKNYTFSQNGKGSIMHRSPNVSPNGRKQSIPLGKMDSVPKTLKLTEAANGLKRGA 300
Query: 302 DVEEFSEITINGTGTKMMEAQANDHKPDIKERLNSVYDSVLVVDSVSAAKEVVSMLTTKY 361
VEEFS++TING GTK+ EA A +HKPDIKERLN VYDSV VVDS+ AA+EVVSMLT KY
Sbjct: 301 AVEEFSKMTINGGGTKITEAPATNHKPDIKERLNGVYDSVHVVDSIRAAREVVSMLTMKY 360
Query: 362 KNLVHACDTEVAKIDVKQETPVDHGEIICFSIYSGPNANFGSGKSCIWVDVLDGGGKEIL 421
+NLVHACDTEVAKIDVKQ TPVDHGEIICFSIYSGP A+FG+GKSCIWVDVLDGGGKEIL
Sbjct: 361 RNLVHACDTEVAKIDVKQATPVDHGEIICFSIYSGPTADFGNGKSCIWVDVLDGGGKEIL 420
Query: 422 LQFAPFFEDPLIKKVWHNYSFDNHIIENYGIKVSGFHADTMHMARLWDSSRRANGGYSLE 481
LQFAPFFEDP I+KVWHNYSFDNHIIENYGIKVSGFHADTMHMARLWDSSRR NGGYSLE
Sbjct: 421 LQFAPFFEDPSIRKVWHNYSFDNHIIENYGIKVSGFHADTMHMARLWDSSRRINGGYSLE 480
Query: 482 ALSGDVKVMSGAKLGHEKELIGKVSMKTIFGRKKMKKDGYEGKLTVIPPVEELQREERRP 541
ALSGD KVMSGAKLG EKELIGK+SMKTIFGRKKMKKDG EGK+ VIPPVEELQREE++
Sbjct: 481 ALSGDTKVMSGAKLGQEKELIGKISMKTIFGRKKMKKDGSEGKIIVIPPVEELQREEKKL 540
Query: 542 WVSYSALDSICTLKLYESLKNKLSNMPWERDGEMIPDKTMFNFYEEYWQPFGELLVKMET 601
WVSYSALDSICTLKLYESLKNKLSNMPWER+GEMIP+KTMFNFYEEYWQPFGELLVKMET
Sbjct: 541 WVSYSALDSICTLKLYESLKNKLSNMPWERNGEMIPNKTMFNFYEEYWQPFGELLVKMET 600
Query: 602 EGMLVDRAYLAEIEKLAKAEQEVAGNRFRNWASKYCPDAKYMNVGSDAQVRQLLFGGTLN 661
EGMLVDR YLA+IEKLA AEQ+VA NRFRNWASKYCPDA++MNVGSDAQ+RQLLFGGT N
Sbjct: 601 EGMLVDRPYLAKIEKLAIAEQQVAANRFRNWASKYCPDARHMNVGSDAQLRQLLFGGTSN 660
Query: 662 SKNPDESLPAERTFKVPNSENVIEEGKKTPGKFRNITLQSILKDKVLSTEMYTASGWPSV 721
SKNPDESLP ERTFKVPN+ENVIEEGKKTP KFRNITL+ I + LSTEMYTA+GWPSV
Sbjct: 661 SKNPDESLPTERTFKVPNTENVIEEGKKTPSKFRNITLKRISVED-LSTEMYTATGWPSV 720
Query: 722 SGDALKILAGKVSAEFDDFTDAHDDLQSDNEVDNDSETMPHGENKKPVIHESANMSDYGT 781
SGDALK+LAGKVSAE+D FTD DLQSDNE +DSET H ENKK +IHESANMSDYG
Sbjct: 721 SGDALKVLAGKVSAEYDYFTD---DLQSDNEFGDDSETTSHEENKKHIIHESANMSDYGA 780
Query: 782 AFEAFASKEEGREACHAIAALCEVCSIDSLISNFILPLQGSNISGKNGRVHCSLNINTET 841
A +AF S E+GREACHAIAALCEVCSIDSLISNFILPLQGSNISGKNGR+HCSLNINTET
Sbjct: 781 ALKAFGSSEKGREACHAIAALCEVCSIDSLISNFILPLQGSNISGKNGRIHCSLNINTET 840
Query: 842 GRLSARRPNLQNQPALEKDRYKIRQAFIAAPGNSLIVADYGQLELRILAHLANCKSMLEA 901
GRLSARRPNLQNQPALEKDRYKIRQAFIAAPGNSLIVADYGQLELRILAHLANCKSMLEA
Sbjct: 841 GRLSARRPNLQNQPALEKDRYKIRQAFIAAPGNSLIVADYGQLELRILAHLANCKSMLEA 900
Query: 902 FKAGGDFHSRTAMNMYPHIRNAVEKGSVLLEWDPQPGEDKPPVPLLKDAFGSERRKAKML 961
FKAGGDFHSRTAMNMYPHIR AVE GSVLLEWDPQPGEDKPPVPLLKDAFGSERRKAKML
Sbjct: 901 FKAGGDFHSRTAMNMYPHIRKAVEDGSVLLEWDPQPGEDKPPVPLLKDAFGSERRKAKML 960
Query: 962 NFSIAYGKTPVGLSKDWKVTVEEARQTVDLWYNERKEVRIWQELRKKEADEKSCVRTLLG 1021
NFSIAYGKTPVGLSKDWKVTVEEA+QTVDLWYNERKEVR WQ LRK+EA+EKSCVRTLLG
Sbjct: 961 NFSIAYGKTPVGLSKDWKVTVEEAKQTVDLWYNERKEVRTWQNLRKREAEEKSCVRTLLG 1020
Query: 1022 RARRFPSMKHATRAQRGHIERAAINTPVQGSAADVAMCAMLEISNNSGLRELGWRLLLQV 1081
RARRFPSM HATRA +GHIERAAINTPVQGSAADVAMCAMLEISNNS LRELGWRLLLQV
Sbjct: 1021 RARRFPSMTHATRAHKGHIERAAINTPVQGSAADVAMCAMLEISNNSRLRELGWRLLLQV 1080
Query: 1082 HDEVILEGPTESAEVAKAIVVECMSKPFSGKNILNVDLAVDAKCAQNWYSAK 1132
HDEVILEGPTESAEVAKAIVVECMSKPF+GKNILNVDLAVDAKCA+NWYSAK
Sbjct: 1081 HDEVILEGPTESAEVAKAIVVECMSKPFNGKNILNVDLAVDAKCARNWYSAK 1126
BLAST of MC10g1035 vs. ExPASy TrEMBL
Match:
A0A6J1D9Z5 (DNA polymerase I A, chloroplastic/mitochondrial OS=Momordica charantia OX=3673 GN=LOC111019012 PE=3 SV=1)
HSP 1 Score: 2247 bits (5822), Expect = 0.0
Identity = 1132/1132 (100.00%), Postives = 1132/1132 (100.00%), Query Frame = 0
Query: 1 MMTLGVSTTQASSLRTSWPSYFFLWRSNSVSNSSISICASSKALYRSEFSSMKSGDGASP 60
MMTLGVSTTQASSLRTSWPSYFFLWRSNSVSNSSISICASSKALYRSEFSSMKSGDGASP
Sbjct: 1 MMTLGVSTTQASSLRTSWPSYFFLWRSNSVSNSSISICASSKALYRSEFSSMKSGDGASP 60
Query: 61 TLNMFHASIQCRKSSFLSTNSLVETRQYDNERAFLSDVNAWSKSTMQIKQEKHFRFMESG 120
TLNMFHASIQCRKSSFLSTNSLVETRQYDNERAFLSDVNAWSKSTMQIKQEKHFRFMESG
Sbjct: 61 TLNMFHASIQCRKSSFLSTNSLVETRQYDNERAFLSDVNAWSKSTMQIKQEKHFRFMESG 120
Query: 121 ILTKSDEEKLRKMENLVGYGTAHSYNRPQYSKVQCFNANKDSDCINPETNRLSDGFRKQE 180
ILTKSDEEKLRKMENLVGYGTAHSYNRPQYSKVQCFNANKDSDCINPETNRLSDGFRKQE
Sbjct: 121 ILTKSDEEKLRKMENLVGYGTAHSYNRPQYSKVQCFNANKDSDCINPETNRLSDGFRKQE 180
Query: 181 PMNFERSVSAATIDRKTDSDRGPSIKTFKVSRGECNGDIDSFSGGRTMNKPENNDLHNQL 240
PMNFERSVSAATIDRKTDSDRGPSIKTFKVSRGECNGDIDSFSGGRTMNKPENNDLHNQL
Sbjct: 181 PMNFERSVSAATIDRKTDSDRGPSIKTFKVSRGECNGDIDSFSGGRTMNKPENNDLHNQL 240
Query: 241 VPMRSNKRYTISQNGKGSISHHAPNVSPNGRKQNISTGKVNNVPKSLKFIEASNEIKRGV 300
VPMRSNKRYTISQNGKGSISHHAPNVSPNGRKQNISTGKVNNVPKSLKFIEASNEIKRGV
Sbjct: 241 VPMRSNKRYTISQNGKGSISHHAPNVSPNGRKQNISTGKVNNVPKSLKFIEASNEIKRGV 300
Query: 301 DVEEFSEITINGTGTKMMEAQANDHKPDIKERLNSVYDSVLVVDSVSAAKEVVSMLTTKY 360
DVEEFSEITINGTGTKMMEAQANDHKPDIKERLNSVYDSVLVVDSVSAAKEVVSMLTTKY
Sbjct: 301 DVEEFSEITINGTGTKMMEAQANDHKPDIKERLNSVYDSVLVVDSVSAAKEVVSMLTTKY 360
Query: 361 KNLVHACDTEVAKIDVKQETPVDHGEIICFSIYSGPNANFGSGKSCIWVDVLDGGGKEIL 420
KNLVHACDTEVAKIDVKQETPVDHGEIICFSIYSGPNANFGSGKSCIWVDVLDGGGKEIL
Sbjct: 361 KNLVHACDTEVAKIDVKQETPVDHGEIICFSIYSGPNANFGSGKSCIWVDVLDGGGKEIL 420
Query: 421 LQFAPFFEDPLIKKVWHNYSFDNHIIENYGIKVSGFHADTMHMARLWDSSRRANGGYSLE 480
LQFAPFFEDPLIKKVWHNYSFDNHIIENYGIKVSGFHADTMHMARLWDSSRRANGGYSLE
Sbjct: 421 LQFAPFFEDPLIKKVWHNYSFDNHIIENYGIKVSGFHADTMHMARLWDSSRRANGGYSLE 480
Query: 481 ALSGDVKVMSGAKLGHEKELIGKVSMKTIFGRKKMKKDGYEGKLTVIPPVEELQREERRP 540
ALSGDVKVMSGAKLGHEKELIGKVSMKTIFGRKKMKKDGYEGKLTVIPPVEELQREERRP
Sbjct: 481 ALSGDVKVMSGAKLGHEKELIGKVSMKTIFGRKKMKKDGYEGKLTVIPPVEELQREERRP 540
Query: 541 WVSYSALDSICTLKLYESLKNKLSNMPWERDGEMIPDKTMFNFYEEYWQPFGELLVKMET 600
WVSYSALDSICTLKLYESLKNKLSNMPWERDGEMIPDKTMFNFYEEYWQPFGELLVKMET
Sbjct: 541 WVSYSALDSICTLKLYESLKNKLSNMPWERDGEMIPDKTMFNFYEEYWQPFGELLVKMET 600
Query: 601 EGMLVDRAYLAEIEKLAKAEQEVAGNRFRNWASKYCPDAKYMNVGSDAQVRQLLFGGTLN 660
EGMLVDRAYLAEIEKLAKAEQEVAGNRFRNWASKYCPDAKYMNVGSDAQVRQLLFGGTLN
Sbjct: 601 EGMLVDRAYLAEIEKLAKAEQEVAGNRFRNWASKYCPDAKYMNVGSDAQVRQLLFGGTLN 660
Query: 661 SKNPDESLPAERTFKVPNSENVIEEGKKTPGKFRNITLQSILKDKVLSTEMYTASGWPSV 720
SKNPDESLPAERTFKVPNSENVIEEGKKTPGKFRNITLQSILKDKVLSTEMYTASGWPSV
Sbjct: 661 SKNPDESLPAERTFKVPNSENVIEEGKKTPGKFRNITLQSILKDKVLSTEMYTASGWPSV 720
Query: 721 SGDALKILAGKVSAEFDDFTDAHDDLQSDNEVDNDSETMPHGENKKPVIHESANMSDYGT 780
SGDALKILAGKVSAEFDDFTDAHDDLQSDNEVDNDSETMPHGENKKPVIHESANMSDYGT
Sbjct: 721 SGDALKILAGKVSAEFDDFTDAHDDLQSDNEVDNDSETMPHGENKKPVIHESANMSDYGT 780
Query: 781 AFEAFASKEEGREACHAIAALCEVCSIDSLISNFILPLQGSNISGKNGRVHCSLNINTET 840
AFEAFASKEEGREACHAIAALCEVCSIDSLISNFILPLQGSNISGKNGRVHCSLNINTET
Sbjct: 781 AFEAFASKEEGREACHAIAALCEVCSIDSLISNFILPLQGSNISGKNGRVHCSLNINTET 840
Query: 841 GRLSARRPNLQNQPALEKDRYKIRQAFIAAPGNSLIVADYGQLELRILAHLANCKSMLEA 900
GRLSARRPNLQNQPALEKDRYKIRQAFIAAPGNSLIVADYGQLELRILAHLANCKSMLEA
Sbjct: 841 GRLSARRPNLQNQPALEKDRYKIRQAFIAAPGNSLIVADYGQLELRILAHLANCKSMLEA 900
Query: 901 FKAGGDFHSRTAMNMYPHIRNAVEKGSVLLEWDPQPGEDKPPVPLLKDAFGSERRKAKML 960
FKAGGDFHSRTAMNMYPHIRNAVEKGSVLLEWDPQPGEDKPPVPLLKDAFGSERRKAKML
Sbjct: 901 FKAGGDFHSRTAMNMYPHIRNAVEKGSVLLEWDPQPGEDKPPVPLLKDAFGSERRKAKML 960
Query: 961 NFSIAYGKTPVGLSKDWKVTVEEARQTVDLWYNERKEVRIWQELRKKEADEKSCVRTLLG 1020
NFSIAYGKTPVGLSKDWKVTVEEARQTVDLWYNERKEVRIWQELRKKEADEKSCVRTLLG
Sbjct: 961 NFSIAYGKTPVGLSKDWKVTVEEARQTVDLWYNERKEVRIWQELRKKEADEKSCVRTLLG 1020
Query: 1021 RARRFPSMKHATRAQRGHIERAAINTPVQGSAADVAMCAMLEISNNSGLRELGWRLLLQV 1080
RARRFPSMKHATRAQRGHIERAAINTPVQGSAADVAMCAMLEISNNSGLRELGWRLLLQV
Sbjct: 1021 RARRFPSMKHATRAQRGHIERAAINTPVQGSAADVAMCAMLEISNNSGLRELGWRLLLQV 1080
Query: 1081 HDEVILEGPTESAEVAKAIVVECMSKPFSGKNILNVDLAVDAKCAQNWYSAK 1132
HDEVILEGPTESAEVAKAIVVECMSKPFSGKNILNVDLAVDAKCAQNWYSAK
Sbjct: 1081 HDEVILEGPTESAEVAKAIVVECMSKPFSGKNILNVDLAVDAKCAQNWYSAK 1132
BLAST of MC10g1035 vs. ExPASy TrEMBL
Match:
A0A6J1FW48 (DNA polymerase I B, chloroplastic/mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111447420 PE=3 SV=1)
HSP 1 Score: 1862 bits (4823), Expect = 0.0
Identity = 943/1133 (83.23%), Postives = 1019/1133 (89.94%), Query Frame = 0
Query: 1 MMTLGVSTTQASSLRTSWPSYFFLWRSNSVSNSSISICASSKALYRSEFSSMKSGDGASP 60
MMTLGVS++QASSLR WP+YFFLWRSNSVS+SSIS CASSKAL R+EF +KS G S
Sbjct: 1 MMTLGVSSSQASSLRAQWPAYFFLWRSNSVSSSSISFCASSKALCRAEFGPLKSVGGVSS 60
Query: 61 TLNMFHASIQCRKSSFLSTNSLVETRQYDNERAFLSDVNAWSKSTMQIKQEKHFRFMESG 120
+ FHAS QCR+SSFLSTNS VETRQYDNERAFLSDV AWSKSTM++K+EKH RFME+
Sbjct: 61 NMKTFHASCQCRQSSFLSTNSFVETRQYDNERAFLSDVKAWSKSTMRLKEEKHLRFMETE 120
Query: 121 ILTKSDEEKLRKMENLVGYGTAHSYNRPQY-SKVQCFNANKDSDCINPETNRLSDGFRKQ 180
ILT +DEEKLR E+L+GYGT+HS + SK+ NANKDSDCINP TN LSDGFRKQ
Sbjct: 121 ILTTNDEEKLRNEEHLIGYGTSHSKIQSNLGSKLS--NANKDSDCINPSTNMLSDGFRKQ 180
Query: 181 EPMNFERSVSAATIDRKTDSDRGPSIKTFKVSRGECNGDIDSFSGGRTMNKPENNDLHNQ 240
PM+FE+ + TI+ SDR S+ T KVS CNG+I S+SG +TM KP NNDLHNQ
Sbjct: 181 GPMSFEQLQNVETIEGMMISDRTLSLDTIKVSSDRCNGNISSYSGEQTMTKPANNDLHNQ 240
Query: 241 LVPMRSNKRYTISQNGKGSISHHAPNVSPNGRKQNISTGKVNNVPKSLKFIEASNEIKRG 300
++PMRSNK YT SQNGKGSI H + NVSPNGRKQ+I GK++++PK+LK EA+N +KRG
Sbjct: 241 VIPMRSNKNYTFSQNGKGSIMHRSSNVSPNGRKQSIPLGKMDSLPKTLKLTEAANGLKRG 300
Query: 301 VDVEEFSEITINGTGTKMMEAQANDHKPDIKERLNSVYDSVLVVDSVSAAKEVVSMLTTK 360
VEEFS++TING GTK+ EA A HKPDIKERLN VYDSVLVVDS+ AA+EVVSMLT K
Sbjct: 301 AAVEEFSKMTINGGGTKITEAPATSHKPDIKERLNGVYDSVLVVDSIQAAREVVSMLTMK 360
Query: 361 YKNLVHACDTEVAKIDVKQETPVDHGEIICFSIYSGPNANFGSGKSCIWVDVLDGGGKEI 420
Y+NLVHACDTEVAKIDVKQETPVDHGEIICFSIYSGP A+FG+GKSCIWVDVLDGGGKEI
Sbjct: 361 YRNLVHACDTEVAKIDVKQETPVDHGEIICFSIYSGPTADFGNGKSCIWVDVLDGGGKEI 420
Query: 421 LLQFAPFFEDPLIKKVWHNYSFDNHIIENYGIKVSGFHADTMHMARLWDSSRRANGGYSL 480
LLQFAPFFEDP I+KVWHNYSFDNHIIENYGIKVSGFHADTMHMARLWDSSRR NGGYSL
Sbjct: 421 LLQFAPFFEDPSIRKVWHNYSFDNHIIENYGIKVSGFHADTMHMARLWDSSRRINGGYSL 480
Query: 481 EALSGDVKVMSGAKLGHEKELIGKVSMKTIFGRKKMKKDGYEGKLTVIPPVEELQREERR 540
EALSGD KVMSGAKLG EKELIGK+SMK+IFGRKKMKKDG EGK+ VIPPVEELQREE++
Sbjct: 481 EALSGDTKVMSGAKLGQEKELIGKISMKSIFGRKKMKKDGSEGKIIVIPPVEELQREEKK 540
Query: 541 PWVSYSALDSICTLKLYESLKNKLSNMPWERDGEMIPDKTMFNFYEEYWQPFGELLVKME 600
WVSYS LDSICTLKLYESLKNKLS+MPWER+GEMIP+KTMFNFYEEYWQPFGELLVKME
Sbjct: 541 LWVSYSGLDSICTLKLYESLKNKLSDMPWERNGEMIPNKTMFNFYEEYWQPFGELLVKME 600
Query: 601 TEGMLVDRAYLAEIEKLAKAEQEVAGNRFRNWASKYCPDAKYMNVGSDAQVRQLLFGGTL 660
TEGMLVDR YLA+IEKLA AEQ+VA NRFRNWASKYCPDA++MNVGSDAQ+RQLLFGGT
Sbjct: 601 TEGMLVDRPYLAKIEKLAIAEQQVAANRFRNWASKYCPDARHMNVGSDAQLRQLLFGGTS 660
Query: 661 NSKNPDESLPAERTFKVPNSENVIEEGKKTPGKFRNITLQSILKDKVLSTEMYTASGWPS 720
NSKNPDESLP ERTFKVPN+ENVIEEGKKTP KFRNI L+ I + LSTEMYTA+GWPS
Sbjct: 661 NSKNPDESLPTERTFKVPNTENVIEEGKKTPSKFRNINLKRISVED-LSTEMYTATGWPS 720
Query: 721 VSGDALKILAGKVSAEFDDFTDAHDDLQSDNEVDNDSETMPHGENKKPVIHESANMSDYG 780
VSGDALK+LAGKVSAE+D FTD DLQSDNE +DSET H ENKK +IHESANMSDYG
Sbjct: 721 VSGDALKVLAGKVSAEYDYFTD---DLQSDNEFGDDSETTSHEENKKHIIHESANMSDYG 780
Query: 781 TAFEAFASKEEGREACHAIAALCEVCSIDSLISNFILPLQGSNISGKNGRVHCSLNINTE 840
A +AF S E+GREACHAIAALCEVCSIDSLISNFILPLQGSNISGKNGR+HCSLNINTE
Sbjct: 781 AALKAFGSSEKGREACHAIAALCEVCSIDSLISNFILPLQGSNISGKNGRIHCSLNINTE 840
Query: 841 TGRLSARRPNLQNQPALEKDRYKIRQAFIAAPGNSLIVADYGQLELRILAHLANCKSMLE 900
TGRLSARRPNLQNQPALEKDRYKIRQAFIAAPGNSLIVADYGQLELRILAHLANCKSMLE
Sbjct: 841 TGRLSARRPNLQNQPALEKDRYKIRQAFIAAPGNSLIVADYGQLELRILAHLANCKSMLE 900
Query: 901 AFKAGGDFHSRTAMNMYPHIRNAVEKGSVLLEWDPQPGEDKPPVPLLKDAFGSERRKAKM 960
AFKAGGDFHSRTAMNMYPHIR AVE GSVLLEWDPQPGEDKPPVPLLKDAFGSERRKAKM
Sbjct: 901 AFKAGGDFHSRTAMNMYPHIRKAVEDGSVLLEWDPQPGEDKPPVPLLKDAFGSERRKAKM 960
Query: 961 LNFSIAYGKTPVGLSKDWKVTVEEARQTVDLWYNERKEVRIWQELRKKEADEKSCVRTLL 1020
LNFSIAYGKTPVGLSKDWKVTVEEA+QTVDLWYNERKEVR WQ LRK+EA+EKSCVRTLL
Sbjct: 961 LNFSIAYGKTPVGLSKDWKVTVEEAKQTVDLWYNERKEVRTWQNLRKREAEEKSCVRTLL 1020
Query: 1021 GRARRFPSMKHATRAQRGHIERAAINTPVQGSAADVAMCAMLEISNNSGLRELGWRLLLQ 1080
GRARRFPSM HATRA +GHIERAAINTPVQGSAADVAMCAMLEISNNS LRELGWRLLLQ
Sbjct: 1021 GRARRFPSMTHATRAHKGHIERAAINTPVQGSAADVAMCAMLEISNNSRLRELGWRLLLQ 1080
Query: 1081 VHDEVILEGPTESAEVAKAIVVECMSKPFSGKNILNVDLAVDAKCAQNWYSAK 1132
VHDEVILEGPTESAEVAKAIVVECMSKPF+GKNILNVDLAVDAKCA+NWYSAK
Sbjct: 1081 VHDEVILEGPTESAEVAKAIVVECMSKPFNGKNILNVDLAVDAKCARNWYSAK 1127
BLAST of MC10g1035 vs. ExPASy TrEMBL
Match:
A0A6J1IIP1 (DNA polymerase I A, chloroplastic/mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111476616 PE=3 SV=1)
HSP 1 Score: 1857 bits (4810), Expect = 0.0
Identity = 942/1132 (83.22%), Postives = 1016/1132 (89.75%), Query Frame = 0
Query: 1 MMTLGVSTTQASSLRTSWPSYFFLWRSNSVSNSSISICASSKALYRSEFSSMKSGDGASP 60
MMTLGVST+QASSLR WP+YFFLWRSNSVS SSIS CASSKAL R+EF +KS G S
Sbjct: 1 MMTLGVSTSQASSLRAQWPAYFFLWRSNSVSISSISFCASSKALCRAEFGPLKSVGGVSS 60
Query: 61 TLNMFHASIQCRKSSFLSTNSLVETRQYDNERAFLSDVNAWSKSTMQIKQEKHFRFMESG 120
+ FHAS QCR+SS L TNS VE RQYDNERAFLSDVNAWSKSTM++KQEKHFRFME+
Sbjct: 61 NMKTFHASCQCRQSSSLITNSFVEARQYDNERAFLSDVNAWSKSTMRLKQEKHFRFMETE 120
Query: 121 ILTKSDEEKLRKMENLVGYGTAHSYNRPQYSKVQCFNANKDSDCINPETNRLSDGFRKQE 180
ILT +DEEKLR E L+GYGT+HS + NANK+SDCINP TN LSDGFRKQ
Sbjct: 121 ILTTNDEEKLRNEERLIGYGTSHSKVQSNLGS-NISNANKNSDCINPSTNMLSDGFRKQG 180
Query: 181 PMNFERSVSAATIDRKTDSDRGPSIKTFKVSRGECNGDIDSFSGGRTMNKPENNDLHNQL 240
PM+FE+ + TIDR SDR S++T KVS CNG+I +SG +TM KP NNDLHNQ+
Sbjct: 181 PMSFEQIQNVETIDRMKISDRTLSLETIKVSSDICNGNISFYSGEQTMTKPANNDLHNQV 240
Query: 241 VPMRSNKRYTISQNGKGSISHHAPNVSPNGRKQNISTGKVNNVPKSLKFIEASNEIKRGV 300
+PM SNK YT SQNGKGSI H +PNVSPNG KQ+I GK+++V K+ +F EA+N +KRG
Sbjct: 241 IPMLSNKNYTFSQNGKGSIMHRSPNVSPNGIKQSIPLGKMDSVSKASEFTEAANGLKRGS 300
Query: 301 DVEEFSEITINGTGTKMMEAQANDHKPDIKERLNSVYDSVLVVDSVSAAKEVVSMLTTKY 360
VEEFS++TING GTK+MEA A +HKPDIKERLN VYDSVLVVDS+ AA+EVVSMLT KY
Sbjct: 301 AVEEFSKMTINGGGTKIMEAPATNHKPDIKERLNGVYDSVLVVDSICAAREVVSMLTMKY 360
Query: 361 KNLVHACDTEVAKIDVKQETPVDHGEIICFSIYSGPNANFGSGKSCIWVDVLDGGGKEIL 420
+NLVHACDTEVAKIDVKQETPVDHGEIICFSIYSGP A+FG+GKSCIWVDVLDGGGKEIL
Sbjct: 361 RNLVHACDTEVAKIDVKQETPVDHGEIICFSIYSGPTADFGNGKSCIWVDVLDGGGKEIL 420
Query: 421 LQFAPFFEDPLIKKVWHNYSFDNHIIENYGIKVSGFHADTMHMARLWDSSRRANGGYSLE 480
+QFAPFFEDP I+KVWHNYSFDNHIIENYGIKVSGFHADTMHMARLWDSSRR NGGYSLE
Sbjct: 421 IQFAPFFEDPSIRKVWHNYSFDNHIIENYGIKVSGFHADTMHMARLWDSSRRINGGYSLE 480
Query: 481 ALSGDVKVMSGAKLGHEKELIGKVSMKTIFGRKKMKKDGYEGKLTVIPPVEELQREERRP 540
ALSGD KVMSGAKLG EKELIGK+SMKTIFGRKKMKKDG EGK+ VIPPVEELQREE++
Sbjct: 481 ALSGDTKVMSGAKLGQEKELIGKISMKTIFGRKKMKKDGSEGKIIVIPPVEELQREEKKL 540
Query: 541 WVSYSALDSICTLKLYESLKNKLSNMPWERDGEMIPDKTMFNFYEEYWQPFGELLVKMET 600
WVSYSALDS CTLKLYESLKNKLS MPWER+GEMIP+KTMFNFYEEYWQPFGELLVKMET
Sbjct: 541 WVSYSALDSTCTLKLYESLKNKLSGMPWERNGEMIPNKTMFNFYEEYWQPFGELLVKMET 600
Query: 601 EGMLVDRAYLAEIEKLAKAEQEVAGNRFRNWASKYCPDAKYMNVGSDAQVRQLLFGGTLN 660
EGMLVDR YLA+IEKLA AEQ+VA NRFRNWASKYCPDA++MNVGSDAQ+RQLLFGGT N
Sbjct: 601 EGMLVDRPYLAKIEKLAIAEQQVAANRFRNWASKYCPDARHMNVGSDAQLRQLLFGGTSN 660
Query: 661 SKNPDESLPAERTFKVPNSENVIEEGKKTPGKFRNITLQSILKDKVLSTEMYTASGWPSV 720
SKNP+ESLP ERTFKVPN+ENVIEEGKK+P KFRNITL+ I + LSTEMYTA+GWPSV
Sbjct: 661 SKNPEESLPTERTFKVPNTENVIEEGKKSPSKFRNITLKRISVED-LSTEMYTATGWPSV 720
Query: 721 SGDALKILAGKVSAEFDDFTDAHDDLQSDNEVDNDSETMPHGENKKPVIHESANMSDYGT 780
SGDALK+LAGKVSAE+D FTD DLQSDNE +DSET+ H ENKK +IHESANMSDYGT
Sbjct: 721 SGDALKVLAGKVSAEYDYFTD---DLQSDNEFGDDSETVSHVENKKHIIHESANMSDYGT 780
Query: 781 AFEAFASKEEGREACHAIAALCEVCSIDSLISNFILPLQGSNISGKNGRVHCSLNINTET 840
A +AF S E+GREACHAIAALCEVCSIDSLISNFILPLQGSNISGKNGR+HCSLNINTET
Sbjct: 781 ALKAFGSSEKGREACHAIAALCEVCSIDSLISNFILPLQGSNISGKNGRIHCSLNINTET 840
Query: 841 GRLSARRPNLQNQPALEKDRYKIRQAFIAAPGNSLIVADYGQLELRILAHLANCKSMLEA 900
GRLSARRPNLQNQPALEKDRYKIRQAFIAAPGNSLIVADYGQLELRILAHLANCKSMLEA
Sbjct: 841 GRLSARRPNLQNQPALEKDRYKIRQAFIAAPGNSLIVADYGQLELRILAHLANCKSMLEA 900
Query: 901 FKAGGDFHSRTAMNMYPHIRNAVEKGSVLLEWDPQPGEDKPPVPLLKDAFGSERRKAKML 960
FKAGGDFHSRTAMNMYPHIR AVE GSVLLEWDPQPGEDKPPVPLLKDAFGSERRKAKML
Sbjct: 901 FKAGGDFHSRTAMNMYPHIRKAVEDGSVLLEWDPQPGEDKPPVPLLKDAFGSERRKAKML 960
Query: 961 NFSIAYGKTPVGLSKDWKVTVEEARQTVDLWYNERKEVRIWQELRKKEADEKSCVRTLLG 1020
NFSIAYGKTPVGLSKDWKVTVEEA+QTVDLWYNERKEVR WQ LRK+EA+EKSCVRTLLG
Sbjct: 961 NFSIAYGKTPVGLSKDWKVTVEEAKQTVDLWYNERKEVRSWQNLRKREAEEKSCVRTLLG 1020
Query: 1021 RARRFPSMKHATRAQRGHIERAAINTPVQGSAADVAMCAMLEISNNSGLRELGWRLLLQV 1080
RARRFPSMKHATRA +GHIERAAINTPVQGSAADVAMCAMLEIS NS LRELGWRLLLQV
Sbjct: 1021 RARRFPSMKHATRAHKGHIERAAINTPVQGSAADVAMCAMLEISKNSRLRELGWRLLLQV 1080
Query: 1081 HDEVILEGPTESAEVAKAIVVECMSKPFSGKNILNVDLAVDAKCAQNWYSAK 1132
HDEVILEGPTESAEVAKAIVVECMSKPF+GKNILNVDLAVDAKCAQNWYSAK
Sbjct: 1081 HDEVILEGPTESAEVAKAIVVECMSKPFNGKNILNVDLAVDAKCAQNWYSAK 1127
BLAST of MC10g1035 vs. ExPASy TrEMBL
Match:
A0A6J1EVX1 (DNA polymerase I A, chloroplastic/mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111438488 PE=3 SV=1)
HSP 1 Score: 1848 bits (4786), Expect = 0.0
Identity = 944/1166 (80.96%), Postives = 1021/1166 (87.56%), Query Frame = 0
Query: 1 MMTLGVSTTQASSLRTSWPSYFFLWRSNSVSNSSISICASSKALYRSEFSSMKSGDGASP 60
M+TLG ST+QASSL T WPSYFFLWRSNSVSNSSISICASSK LYR+EFSS+KS ASP
Sbjct: 1 MITLGASTSQASSLITQWPSYFFLWRSNSVSNSSISICASSKRLYRAEFSSLKSVRSASP 60
Query: 61 TLNMFHASIQCRKSSFLSTNSLVETRQYDNERAFLSDVNAWSKSTMQIKQEKHFRFMESG 120
+NMFHAS+QCR+SSFL TNS ETRQ+D ERAFLSD+N WSKST Q+KQEK FRF E+
Sbjct: 61 NVNMFHASLQCRQSSFLCTNSFFETRQHDKERAFLSDINDWSKSTRQLKQEKLFRFSETE 120
Query: 121 ILTKSDEEKLRKMENLVGYGTAHSYNR--PQYSKVQ------CFNANKDSDCINPETNRL 180
ILTK+DEEKLRK ENL+GYGT H YN P YSKVQ C NA+ D +CINP TN L
Sbjct: 121 ILTKNDEEKLRKKENLIGYGTLHCYNSLCPPYSKVQTNLGSNCSNASNDPNCINPPTNVL 180
Query: 181 SDGFRKQEPMNFERSVSAATIDRKTDSDRGPSIKTFKVSRGECNGDIDSFSGGRTMNKPE 240
SD F KQEP+NFER+ + ATIDR SDR P ++T K SRGECNGD +S+SG R M+KP
Sbjct: 181 SDEFGKQEPINFERTENVATIDRMI-SDRVPLLETVKFSRGECNGDTNSYSGERPMSKPA 240
Query: 241 NNDLHNQLVPMRSNKRYTISQNGKGSISHHAPNVSPNGRKQNISTGKVNNVPKSLKFIEA 300
NN LH+Q+VPM+SNK+Y++SQNGKGSI H PNVSP GR +NIS GKVN+V K+ K EA
Sbjct: 241 NNVLHSQVVPMQSNKKYSVSQNGKGSIMRHVPNVSPKGRNRNISLGKVNSVLKTSKCTEA 300
Query: 301 SNEIKRGVDVEEFSEITINGTGTKMMEAQANDHKPDIKERLN------------------ 360
+N I +GV EEFS++ +NG+GTKMME A HKPDIKERLN
Sbjct: 301 ANGINKGVAGEEFSKVIVNGSGTKMMEVLATAHKPDIKERLNGVYESVLVVDGVSAAKEV 360
Query: 361 --------SVYDSVLVVDSVSAAKEVVSMLTTKYKNLVHACDTEVAKIDVKQETPVDHGE 420
SVY+SVLVVD VSAAKEVVSMLTTKYKNLVHACDTEVAKIDVKQETPVDHGE
Sbjct: 361 VSIKERLNSVYESVLVVDGVSAAKEVVSMLTTKYKNLVHACDTEVAKIDVKQETPVDHGE 420
Query: 421 IICFSIYSGPNANFGSGKSCIWVDVLDGGGKEILLQFAPFFEDPLIKKVWHNYSFDNHII 480
IICFSIYSGP A+FG+GKSCIWVDVLDGGGKEILLQFAPFFEDPLI+KVWHNYSFDNHII
Sbjct: 421 IICFSIYSGPKADFGNGKSCIWVDVLDGGGKEILLQFAPFFEDPLIRKVWHNYSFDNHII 480
Query: 481 ENYGIKVSGFHADTMHMARLWDSSRRANGGYSLEALSGDVKVMSGAKLGHEKELIGKVSM 540
ENYGIK+SGFHADTMHMARLWDSSRR NGGYSLEALS D KVMSGAKL EKELIGKVSM
Sbjct: 481 ENYGIKISGFHADTMHMARLWDSSRRMNGGYSLEALSCDTKVMSGAKLDQEKELIGKVSM 540
Query: 541 KTIFGRKKMKKDGYEGKLTVIPPVEELQREERRPWVSYSALDSICTLKLYESLKNKLSNM 600
KTIFGRKKMKKDG EGKL VIPPVEELQREER+PWVSYSALDSICTLKLYESLK KLS+M
Sbjct: 541 KTIFGRKKMKKDGSEGKLIVIPPVEELQREERKPWVSYSALDSICTLKLYESLKKKLSDM 600
Query: 601 PWERDGEMIPDKTMFNFYEEYWQPFGELLVKMETEGMLVDRAYLAEIEKLAKAEQEVAGN 660
PWERDGE IPDKTMFNFYE+YW+PFGE+LV+METEGMLVDR YLAEIEKLAKAE EVA N
Sbjct: 601 PWERDGERIPDKTMFNFYEDYWKPFGEVLVRMETEGMLVDRPYLAEIEKLAKAEHEVAAN 660
Query: 661 RFRNWASKYCPDAKYMNVGSDAQVRQLLFGGTLNSKNPDESLPAERTFKVPNSENVIEEG 720
RFRNWAS+YCPDAKYMNVGSDAQVRQLLFGGT NSKNP+ESLP ERTFK+PNSE V EEG
Sbjct: 661 RFRNWASEYCPDAKYMNVGSDAQVRQLLFGGTCNSKNPEESLPTERTFKIPNSEKVTEEG 720
Query: 721 KKTPGKFRNITLQSILKDKVLSTEMYTASGWPSVSGDALKILAGKVSAEFDDFTDAHDDL 780
KKTP KFRNITL+ D+ LSTE+YTA+GWPSVSGDALKILAGKVSAEFDDFTD D
Sbjct: 721 KKTPSKFRNITLRRF-SDEALSTELYTATGWPSVSGDALKILAGKVSAEFDDFTD---DP 780
Query: 781 QSDNEVDNDSETMPHGENKKPVIHESANMSDYGTAFEAFASKEEGREACHAIAALCEVCS 840
QSD EV ND ETMPH EN++ ++HE ANMSDYGTA +AF KEEG EACHAIAALCE+CS
Sbjct: 781 QSDTEVVNDFETMPHEENRRRIVHECANMSDYGTALKAFKLKEEGMEACHAIAALCEICS 840
Query: 841 IDSLISNFILPLQGSNISGKNGRVHCSLNINTETGRLSARRPNLQNQPALEKDRYKIRQA 900
IDSLISNFILPLQGSNISGKNGRVHCSLNINTETGRLSARRP+LQNQPALEKDRYKIRQA
Sbjct: 841 IDSLISNFILPLQGSNISGKNGRVHCSLNINTETGRLSARRPSLQNQPALEKDRYKIRQA 900
Query: 901 FIAAPGNSLIVADYGQLELRILAHLANCKSMLEAFKAGGDFHSRTAMNMYPHIRNAVEKG 960
FIA+PGNSLIVADYGQLELRILAHLANC+SML+AFKAGGDFHSRTAMNMYPHIR AVE+G
Sbjct: 901 FIASPGNSLIVADYGQLELRILAHLANCQSMLDAFKAGGDFHSRTAMNMYPHIRKAVEEG 960
Query: 961 SVLLEWDPQPGEDKPPVPLLKDAFGSERRKAKMLNFSIAYGKTPVGLSKDWKVTVEEARQ 1020
SVLLEWDPQPGEDKPPVPLLKDAF SERRKAKMLNFSIAYGKTP+GLSKDWKVTVEEA +
Sbjct: 961 SVLLEWDPQPGEDKPPVPLLKDAFASERRKAKMLNFSIAYGKTPIGLSKDWKVTVEEASK 1020
Query: 1021 TVDLWYNERKEVRIWQELRKKEADEKSCVRTLLGRARRFPSMKHATRAQRGHIERAAINT 1080
TVDLWYNER EVR WQELR++E ++KSCVRTLLGRAR+FPSMK TRAQ+GHIERAAINT
Sbjct: 1021 TVDLWYNERTEVRRWQELRREETEKKSCVRTLLGRARQFPSMKQVTRAQKGHIERAAINT 1080
Query: 1081 PVQGSAADVAMCAMLEISNNSGLRELGWRLLLQVHDEVILEGPTESAEVAKAIVVECMSK 1132
PVQGSAADVAMCAMLEIS NS LRELGWRLLLQVHDEVILEGPTESAEVAKAIVV+CMSK
Sbjct: 1081 PVQGSAADVAMCAMLEISKNSRLRELGWRLLLQVHDEVILEGPTESAEVAKAIVVDCMSK 1140
BLAST of MC10g1035 vs. ExPASy TrEMBL
Match:
A0A6J1J7E3 (DNA polymerase I A, chloroplastic/mitochondrial isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483260 PE=3 SV=1)
HSP 1 Score: 1833 bits (4748), Expect = 0.0
Identity = 930/1140 (81.58%), Postives = 1010/1140 (88.60%), Query Frame = 0
Query: 1 MMTLGVSTTQASSLRTSWPSYFFLWRSNSVSNSSISICASSKALYRSEFSSMKSGDGASP 60
M+TLG ST+QASSL T WPSYFFLWRSNSVSNSSISICASSK LYR+EF +K+ SP
Sbjct: 1 MITLGASTSQASSLITQWPSYFFLWRSNSVSNSSISICASSKRLYRAEFCPLKNVGSTSP 60
Query: 61 TLNMFHASIQCRKSSFLSTNSLVETRQYDNERAFLSDVNAWSKSTMQIKQEKHFRFMESG 120
+NMFHAS+QCR+SSFL TNS ETRQ+D ERAFLSDVN WSKST Q+KQEK FRF E+
Sbjct: 61 NVNMFHASLQCRQSSFLHTNSFFETRQHDKERAFLSDVNDWSKSTRQLKQEKLFRFSETE 120
Query: 121 ILTKSDEEKLRKMENLVGYGTAHSYNR--PQYSKVQ------CFNANKDSDCINPETNRL 180
ILTK+DEEKLRK ENL+ YGT H YN P YSKVQ C NA D +CINP TN L
Sbjct: 121 ILTKNDEEKLRKKENLIDYGTLHCYNSLSPPYSKVQSNLGSNCSNACNDPNCINPPTNML 180
Query: 181 SDGFRKQEPMNFERSVSAATIDRKTDSDRGPSIKTFKVSRGECNGDIDSFSGGRTMNKPE 240
SD F +QEP+NFER+ + IDR SDR P ++T K SRGECNGD +S+SG +M+KP
Sbjct: 181 SDEFSRQEPINFERTENVTAIDRMI-SDRVPLLETVKFSRGECNGDTNSYSGEWSMSKPA 240
Query: 241 NNDLHNQLVPMRSNKRYTISQNGKGSISHHAPNVSPNGRKQNISTGKVNNVPKSLKFIEA 300
NN LH+Q+VP++SNK+Y++SQNGKG I H PNVSPNGR +NIS GKVN+V K+ KF EA
Sbjct: 241 NNVLHSQVVPIQSNKKYSVSQNGKGLIIRHVPNVSPNGRNRNISLGKVNSVRKTSKFTEA 300
Query: 301 SNEIKRGVDVEEFSEITINGTGTKMMEAQANDHKPDIKERLNSVYDSVLVVDSVSAAKEV 360
+N I +GV VEEFS++ +NG+ TKMME A KPDIKERLN VY+SVLVVD VSAAKEV
Sbjct: 301 ANGINKGVAVEEFSKVIVNGSVTKMMEVLATADKPDIKERLNGVYESVLVVDGVSAAKEV 360
Query: 361 VSMLTTKYKNLVHACDTEVAKIDVKQETPVDHGEIICFSIYSGPNANFGSGKSCIWVDVL 420
VSMLTTKYKNLVHACDTEVAKIDVKQETPVDHGEIICFSIYSGP A+FG+GKSCIWVDVL
Sbjct: 361 VSMLTTKYKNLVHACDTEVAKIDVKQETPVDHGEIICFSIYSGPKADFGNGKSCIWVDVL 420
Query: 421 DGGGKEILLQFAPFFEDPLIKKVWHNYSFDNHIIENYGIKVSGFHADTMHMARLWDSSRR 480
DGGGKEIL QFAPFFEDPLI+KVWHNYSFDNHIIENYGIK+SGFHADTMHMARLWDSSRR
Sbjct: 421 DGGGKEILRQFAPFFEDPLIRKVWHNYSFDNHIIENYGIKISGFHADTMHMARLWDSSRR 480
Query: 481 ANGGYSLEALSGDVKVMSGAKLGHEKELIGKVSMKTIFGRKKMKKDGYEGKLTVIPPVEE 540
NGGYSLEALS D KVMSGAKLG EKELIGKVSMKTIFGRKKMKKDG EGKL VIPPVEE
Sbjct: 481 MNGGYSLEALSYDTKVMSGAKLGQEKELIGKVSMKTIFGRKKMKKDGSEGKLIVIPPVEE 540
Query: 541 LQREERRPWVSYSALDSICTLKLYESLKNKLSNMPWERDGEMIPDKTMFNFYEEYWQPFG 600
LQREER+PWVSYSALDSICTLKLYESLK LS+MPWERDGE IPDKTMFNFYE+YW+PFG
Sbjct: 541 LQREERKPWVSYSALDSICTLKLYESLKKTLSDMPWERDGERIPDKTMFNFYEDYWKPFG 600
Query: 601 ELLVKMETEGMLVDRAYLAEIEKLAKAEQEVAGNRFRNWASKYCPDAKYMNVGSDAQVRQ 660
E+LV+METEGMLVDR YLAEIEKLAKAEQE+A NRFRNWASKYCPDA+YMNVGSDAQ+RQ
Sbjct: 601 EVLVRMETEGMLVDRPYLAEIEKLAKAEQEIAANRFRNWASKYCPDARYMNVGSDAQLRQ 660
Query: 661 LLFGGTLNSKNPDESLPAERTFKVPNSENVIEEGKKTPGKFRNITLQSILKDKVLSTEMY 720
LLFGGT NSKNP+ESLP ERTFK+PNSE V EEGKKTP KFRNITL+ D+ LSTE+Y
Sbjct: 661 LLFGGTCNSKNPEESLPTERTFKIPNSEKVTEEGKKTPSKFRNITLRRF-SDEALSTELY 720
Query: 721 TASGWPSVSGDALKILAGKVSAEFDDFTDAHDDLQSDNEVDNDSETMPHGENKKPVIHES 780
TA+GWPSVS DALKILAGKVSAEFDDFTD + QSD EV ND ETMP EN++ +IHE
Sbjct: 721 TATGWPSVSRDALKILAGKVSAEFDDFTD---NSQSDTEVVNDFETMPREENRRRIIHEC 780
Query: 781 ANMSDYGTAFEAFASKEEGREACHAIAALCEVCSIDSLISNFILPLQGSNISGKNGRVHC 840
ANMSDYGT AF KEEG EACHAI+ALCE+CSIDSLISNFILPLQGSNISGKNGRVHC
Sbjct: 781 ANMSDYGTTLTAFKLKEEGMEACHAISALCEICSIDSLISNFILPLQGSNISGKNGRVHC 840
Query: 841 SLNINTETGRLSARRPNLQNQPALEKDRYKIRQAFIAAPGNSLIVADYGQLELRILAHLA 900
SLNINTETGRLSARRP+LQNQPALEKDRYKIRQAFIA+PGNSLIVADYGQLELRILAHLA
Sbjct: 841 SLNINTETGRLSARRPSLQNQPALEKDRYKIRQAFIASPGNSLIVADYGQLELRILAHLA 900
Query: 901 NCKSMLEAFKAGGDFHSRTAMNMYPHIRNAVEKGSVLLEWDPQPGEDKPPVPLLKDAFGS 960
NCKSML+AFKAGGDFHSRTAMNMYPHIR AVE+GSVLLEWDPQPGEDKPPVPLLKDAF S
Sbjct: 901 NCKSMLDAFKAGGDFHSRTAMNMYPHIRKAVEEGSVLLEWDPQPGEDKPPVPLLKDAFAS 960
Query: 961 ERRKAKMLNFSIAYGKTPVGLSKDWKVTVEEARQTVDLWYNERKEVRIWQELRKKEADEK 1020
ERRKAKMLNFSIAYGKTP+GLSKDWKVTVEEA +TVDLWYNER EVR WQELR++EA++K
Sbjct: 961 ERRKAKMLNFSIAYGKTPIGLSKDWKVTVEEASKTVDLWYNERTEVRRWQELRREEAEKK 1020
Query: 1021 SCVRTLLGRARRFPSMKHATRAQRGHIERAAINTPVQGSAADVAMCAMLEISNNSGLREL 1080
SCVRTLLGRAR+FPSMK TRAQ+GHIERAAINTPVQGSAADVAMCAMLEIS NS LREL
Sbjct: 1021 SCVRTLLGRARQFPSMKQVTRAQKGHIERAAINTPVQGSAADVAMCAMLEISKNSRLREL 1080
Query: 1081 GWRLLLQVHDEVILEGPTESAEVAKAIVVECMSKPFSGKNILNVDLAVDAKCAQNWYSAK 1132
GWRLLLQVHDEVILEGPTESAEVAKAIVV+CMSKPF+GKNIL VDLAVDAKCAQNWYSAK
Sbjct: 1081 GWRLLLQVHDEVILEGPTESAEVAKAIVVDCMSKPFNGKNILKVDLAVDAKCAQNWYSAK 1135
BLAST of MC10g1035 vs. TAIR 10
Match:
AT1G50840.1 (polymerase gamma 2 )
HSP 1 Score: 1181.8 bits (3056), Expect = 0.0e+00
Identity = 582/833 (69.87%), Postives = 686/833 (82.35%), Query Frame = 0
Query: 302 VEEFSEITING--TGTKMMEAQANDHKPDIKERLNSVYDSVLVVDSVSAAKEVVSMLTTK 361
+ + S T NG + + + + +++E L +YD VL+VD+V AAK+ V+ L +
Sbjct: 226 ISDKSSGTANGNKNTVAISKVERSTEPSNVRENLGKIYDKVLIVDNVQAAKDTVAKLVNQ 285
Query: 362 YKNLVHACDTEVAKIDVKQETPVDHGEIICFSIYSGPNANFGSGKSCIWVDVLDGGGKEI 421
++N VH+CDTEV+ I+VK+ETPVDHGE+ICFSIY GP A+FG+GKSCIWVDVL G+E+
Sbjct: 286 FRNHVHSCDTEVSGIEVKEETPVDHGELICFSIYCGPEADFGNGKSCIWVDVLGENGREV 345
Query: 422 LLQFAPFFEDPLIKKVWHNYSFDNHIIENYGIKVSGFHADTMHMARLWDSSRRANGGYSL 481
L +F P+FED I+KVWHNYSFD+HII N+GI++SGFHADTMHMARLWDS+RR GGYSL
Sbjct: 346 LAEFKPYFEDSFIRKVWHNYSFDSHIIRNHGIEISGFHADTMHMARLWDSARRIKGGYSL 405
Query: 482 EALSGDVKVMSGAKLGHEKELIGKVSMKTIFGRKKMKKDGYEGKLTVIPPVEELQREERR 541
EAL+ D KV+ G + E E +GK+SMKTIFG++K+KKDG EGK+ VIPPVEELQRE+R
Sbjct: 406 EALTSDPKVLGGTQTKEEAEFLGKISMKTIFGKRKLKKDGSEGKIVVIPPVEELQREDRE 465
Query: 542 PWVSYSALDSICTLKLYESLKNKLSNMPWERDGEMIPDKTMFNFYEEYWQPFGELLVKME 601
W+SYSALD+I TLKLYES+ KL M W DG+ + +TM +FY E+W+PFGELLVKME
Sbjct: 466 AWISYSALDAISTLKLYESMTKKLQLMDWHLDGKPVLGRTMLDFYHEFWRPFGELLVKME 525
Query: 602 TEGMLVDRAYLAEIEKLAKAEQEVAGNRFRNWASKYCPDAKYMNVGSDAQVRQLLFGGTL 661
EG+LVDR YLAEIEK+AKAEQ+VAG+RFRNWASKYCPDAKYMN+GSD Q+RQL FGG
Sbjct: 526 AEGILVDREYLAEIEKVAKAEQQVAGSRFRNWASKYCPDAKYMNIGSDTQLRQLFFGGIS 585
Query: 662 NSKNPDESLPAERTFKVPNSENVIEEGKKTPGKFRNITLQSILKDKVLSTEMYTASGWPS 721
NS DE LP E+ FKVPN + VIEEGKKTP KFRNI L I D LSTE +TASGWPS
Sbjct: 586 NSH--DEVLPVEKLFKVPNIDKVIEEGKKTPTKFRNIKLHRI-SDSPLSTENFTASGWPS 645
Query: 722 VSGDALKILAGKVSAEFDDFTDAHDDLQSDNEVDNDSETMPHGENKKPVIHESANMSDYG 781
V GD LK LAGKVSAE+D D D + D+D ET E +K + + S YG
Sbjct: 646 VGGDVLKELAGKVSAEYDFMDDVSDISLEEVVEDDDVET---SETQKSKTDDETDTSAYG 705
Query: 782 TAFEAFASKEEGREACHAIAALCEVCSIDSLISNFILPLQGSNISGKNGRVHCSLNINTE 841
TA+ AF E G+EACHAIA+LCEVCSIDSLISNFILPLQGSN+SGK+GRVHCSLNINTE
Sbjct: 706 TAYVAFGGGERGKEACHAIASLCEVCSIDSLISNFILPLQGSNVSGKDGRVHCSLNINTE 765
Query: 842 TGRLSARRPNLQNQPALEKDRYKIRQAFIAAPGNSLIVADYGQLELRILAHLANCKSMLE 901
TGRLSARRPNLQNQPALEKDRYKIR+AF+A+PGN+L+VADYGQLELRILAHL CKSM+E
Sbjct: 766 TGRLSARRPNLQNQPALEKDRYKIRKAFVASPGNTLVVADYGQLELRILAHLTGCKSMME 825
Query: 902 AFKAGGDFHSRTAMNMYPHIRNAVEKGSVLLEWDPQPGEDKPPVPLLKDAFGSERRKAKM 961
AFKAGGDFHSRTAMNMYPH+R AVE G V+LEW P+PGEDKPPVPLLKDAFGSERRKAKM
Sbjct: 826 AFKAGGDFHSRTAMNMYPHVREAVENGQVILEWHPEPGEDKPPVPLLKDAFGSERRKAKM 885
Query: 962 LNFSIAYGKTPVGLSKDWKVTVEEARQTVDLWYNERKEVRIWQELRKKEADEKSCVRTLL 1021
LNFSIAYGKT VGLS+DWKV+ +EA++TVDLWYN+R+EVR WQE+RKKEA E V TLL
Sbjct: 886 LNFSIAYGKTAVGLSRDWKVSTKEAQETVDLWYNDRQEVRKWQEMRKKEAIEDGYVLTLL 945
Query: 1022 GRARRFPSMKHATRAQRGHIERAAINTPVQGSAADVAMCAMLEISNNSGLRELGWRLLLQ 1081
GR+RRFP+ K +RAQR HI+RAAINTPVQGSAADVAMCAMLEIS N L++LGWRLLLQ
Sbjct: 946 GRSRRFPASK--SRAQRNHIQRAAINTPVQGSAADVAMCAMLEISINQQLKKLGWRLLLQ 1005
Query: 1082 VHDEVILEGPTESAEVAKAIVVECMSKPFSGKNILNVDLAVDAKCAQNWYSAK 1133
+HDEVILEGP ESAE+AK IVV+CMSKPF+G+NIL+VDL+VDAKCAQNWY+AK
Sbjct: 1006 IHDEVILEGPIESAEIAKDIVVDCMSKPFNGRNILSVDLSVDAKCAQNWYAAK 1050
BLAST of MC10g1035 vs. TAIR 10
Match:
AT3G20540.1 (polymerase gamma 1 )
HSP 1 Score: 1167.1 bits (3018), Expect = 0.0e+00
Identity = 585/806 (72.58%), Postives = 672/806 (83.37%), Query Frame = 0
Query: 329 IKERLNSVYDSVLVVDSVSAAKEVVSMLTTKYKNLVHACDTEVAKIDVKQETPVDHGEII 388
++ L +Y+ V VVD+VS+AKE V++L +Y+NLVHACDTEV++IDVK ETPVDHGE+I
Sbjct: 233 VRANLKKIYNRVRVVDNVSSAKETVALLMNQYRNLVHACDTEVSRIDVKTETPVDHGEMI 292
Query: 389 CFSIYSGPNANFGSGKSCIWVDVLDGGGKEILLQFAPFFEDPLIKKVWHNYSFDNHIIEN 448
CFSIY G A+FG GKSCIWVDVL G++IL +F PFFED IKKVWHNYSFDNHII N
Sbjct: 293 CFSIYCGSEADFGDGKSCIWVDVLGENGRDILAEFKPFFEDSSIKKVWHNYSFDNHIIRN 352
Query: 449 YGIKVSGFHADTMHMARLWDSSRRANGGYSLEALSGDVKVMSGAKLGHEKELIGKVSMKT 508
YGIK+SGFH DTMHMARLWDSSRR +GGYSLEAL+ D KV+ G + E EL GK+SMK
Sbjct: 353 YGIKLSGFHGDTMHMARLWDSSRRISGGYSLEALTSDPKVLGGTETKEEAELFGKISMKK 412
Query: 509 IFGRKKMKKDGYEGKLTVIPPVEELQREERRPWVSYSALDSICTLKLYESLKNKLSNMPW 568
IFG+ K+KKDG EGKL +IPPV+ELQ E+R W+SYSALDSI TLKLYES+K +L W
Sbjct: 413 IFGKGKLKKDGSEGKLVIIPPVKELQMEDREAWISYSALDSISTLKLYESMKKQLQAKKW 472
Query: 569 ERDGEMIPDKTMFNFYEEYWQPFGELLVKMETEGMLVDRAYLAEIEKLAKAEQEVAGNRF 628
DG++I K MF+FY+EYWQPFGELL KME+EGMLVDR YLA+IE +AKAEQE+A +RF
Sbjct: 473 FLDGKLISKKNMFDFYQEYWQPFGELLAKMESEGMLVDRDYLAQIEIVAKAEQEIAVSRF 532
Query: 629 RNWASKYCPDAKYMNVGSDAQVRQLLFGGTLNSKNPDESLPAERTFKVPNSENVIEEGKK 688
RNWASK+CPDAK+MNVGSD Q+RQL FGG NS N DE LP E+ FKVPN + VIEEGKK
Sbjct: 533 RNWASKHCPDAKHMNVGSDTQLRQLFFGGISNSCN-DEDLPYEKLFKVPNVDKVIEEGKK 592
Query: 689 TPGKFRNITLQSILKDKVLSTEMYTASGWPSVSGDALKILAGKVSAEFDDFTDAHDDLQS 748
KFRNI L I D+ L TE +TASGWPSVSGD LK LAGKVSAE+D D
Sbjct: 593 RATKFRNIKLHRI-SDRPLPTEKFTASGWPSVSGDTLKALAGKVSAEYDYMEGVLDTCLE 652
Query: 749 DNEVDNDSETMPHGENKKPVIHES--ANMSDYGTAFEAFASKEEGREACHAIAALCEVCS 808
+N D+D ++P + ++ S ++ S YGTAF+AF E G+EACHAIAALCEVCS
Sbjct: 653 ENIGDDDCISLPDEVVETQHVNTSVESDTSAYGTAFDAFGGGESGKEACHAIAALCEVCS 712
Query: 809 IDSLISNFILPLQGSNISGKNGRVHCSLNINTETGRLSARRPNLQNQPALEKDRYKIRQA 868
IDSLISNFILPLQGSN+SGK+GRVHCSLNINTETGRLSARRPNLQNQPALEKDRYKIRQA
Sbjct: 713 IDSLISNFILPLQGSNVSGKDGRVHCSLNINTETGRLSARRPNLQNQPALEKDRYKIRQA 772
Query: 869 FIAAPGNSLIVADYGQLELRILAHLANCKSMLEAFKAGGDFHSRTAMNMYPHIRNAVEKG 928
FIA+PGNSLIVADYGQLELRILAHLA+C+SM EAF AGGDFHSRTAMNMYPHIR AVE G
Sbjct: 773 FIASPGNSLIVADYGQLELRILAHLASCESMKEAFIAGGDFHSRTAMNMYPHIREAVENG 832
Query: 929 SVLLEWDPQPGEDKPPVPLLKDAFGSERRKAKMLNFSIAYGKTPVGLSKDWKVTVEEARQ 988
VLLEW PQPG++KPPVPLLKDAF SERRKAKMLNFSIAYGKT +GLS+DWKV+ EEA+
Sbjct: 833 EVLLEWHPQPGQEKPPVPLLKDAFASERRKAKMLNFSIAYGKTAIGLSRDWKVSREEAQD 892
Query: 989 TVDLWYNERKEVRIWQELRKKEADEKSCVRTLLGRARRFPSMKHATRAQRGHIERAAINT 1048
TV+LWYN+R+EVR WQELRKKEA +K V TLLGRAR+FP ++ +RAQ+ HIERAAINT
Sbjct: 893 TVNLWYNDRQEVRKWQELRKKEAIQKGYVLTLLGRARKFP--EYRSRAQKNHIERAAINT 952
Query: 1049 PVQGSAADVAMCAMLEISNNSGLRELGWRLLLQVHDEVILEGPTESAEVAKAIVVECMSK 1108
PVQGSAADVAMCAMLEISNN L+ELGW+LLLQVHDEVILEGP+ESAE AK IVV CMS+
Sbjct: 953 PVQGSAADVAMCAMLEISNNQRLKELGWKLLLQVHDEVILEGPSESAENAKDIVVNCMSE 1012
Query: 1109 PFSGKNILNVDLAVDAKCAQNWYSAK 1133
PF+GKNIL+VDL+VDAKCAQNWY+ K
Sbjct: 1013 PFNGKNILSVDLSVDAKCAQNWYAGK 1034
BLAST of MC10g1035 vs. TAIR 10
Match:
AT3G20540.2 (polymerase gamma 1 )
HSP 1 Score: 1167.1 bits (3018), Expect = 0.0e+00
Identity = 585/806 (72.58%), Postives = 672/806 (83.37%), Query Frame = 0
Query: 329 IKERLNSVYDSVLVVDSVSAAKEVVSMLTTKYKNLVHACDTEVAKIDVKQETPVDHGEII 388
++ L +Y+ V VVD+VS+AKE V++L +Y+NLVHACDTEV++IDVK ETPVDHGE+I
Sbjct: 248 VRANLKKIYNRVRVVDNVSSAKETVALLMNQYRNLVHACDTEVSRIDVKTETPVDHGEMI 307
Query: 389 CFSIYSGPNANFGSGKSCIWVDVLDGGGKEILLQFAPFFEDPLIKKVWHNYSFDNHIIEN 448
CFSIY G A+FG GKSCIWVDVL G++IL +F PFFED IKKVWHNYSFDNHII N
Sbjct: 308 CFSIYCGSEADFGDGKSCIWVDVLGENGRDILAEFKPFFEDSSIKKVWHNYSFDNHIIRN 367
Query: 449 YGIKVSGFHADTMHMARLWDSSRRANGGYSLEALSGDVKVMSGAKLGHEKELIGKVSMKT 508
YGIK+SGFH DTMHMARLWDSSRR +GGYSLEAL+ D KV+ G + E EL GK+SMK
Sbjct: 368 YGIKLSGFHGDTMHMARLWDSSRRISGGYSLEALTSDPKVLGGTETKEEAELFGKISMKK 427
Query: 509 IFGRKKMKKDGYEGKLTVIPPVEELQREERRPWVSYSALDSICTLKLYESLKNKLSNMPW 568
IFG+ K+KKDG EGKL +IPPV+ELQ E+R W+SYSALDSI TLKLYES+K +L W
Sbjct: 428 IFGKGKLKKDGSEGKLVIIPPVKELQMEDREAWISYSALDSISTLKLYESMKKQLQAKKW 487
Query: 569 ERDGEMIPDKTMFNFYEEYWQPFGELLVKMETEGMLVDRAYLAEIEKLAKAEQEVAGNRF 628
DG++I K MF+FY+EYWQPFGELL KME+EGMLVDR YLA+IE +AKAEQE+A +RF
Sbjct: 488 FLDGKLISKKNMFDFYQEYWQPFGELLAKMESEGMLVDRDYLAQIEIVAKAEQEIAVSRF 547
Query: 629 RNWASKYCPDAKYMNVGSDAQVRQLLFGGTLNSKNPDESLPAERTFKVPNSENVIEEGKK 688
RNWASK+CPDAK+MNVGSD Q+RQL FGG NS N DE LP E+ FKVPN + VIEEGKK
Sbjct: 548 RNWASKHCPDAKHMNVGSDTQLRQLFFGGISNSCN-DEDLPYEKLFKVPNVDKVIEEGKK 607
Query: 689 TPGKFRNITLQSILKDKVLSTEMYTASGWPSVSGDALKILAGKVSAEFDDFTDAHDDLQS 748
KFRNI L I D+ L TE +TASGWPSVSGD LK LAGKVSAE+D D
Sbjct: 608 RATKFRNIKLHRI-SDRPLPTEKFTASGWPSVSGDTLKALAGKVSAEYDYMEGVLDTCLE 667
Query: 749 DNEVDNDSETMPHGENKKPVIHES--ANMSDYGTAFEAFASKEEGREACHAIAALCEVCS 808
+N D+D ++P + ++ S ++ S YGTAF+AF E G+EACHAIAALCEVCS
Sbjct: 668 ENIGDDDCISLPDEVVETQHVNTSVESDTSAYGTAFDAFGGGESGKEACHAIAALCEVCS 727
Query: 809 IDSLISNFILPLQGSNISGKNGRVHCSLNINTETGRLSARRPNLQNQPALEKDRYKIRQA 868
IDSLISNFILPLQGSN+SGK+GRVHCSLNINTETGRLSARRPNLQNQPALEKDRYKIRQA
Sbjct: 728 IDSLISNFILPLQGSNVSGKDGRVHCSLNINTETGRLSARRPNLQNQPALEKDRYKIRQA 787
Query: 869 FIAAPGNSLIVADYGQLELRILAHLANCKSMLEAFKAGGDFHSRTAMNMYPHIRNAVEKG 928
FIA+PGNSLIVADYGQLELRILAHLA+C+SM EAF AGGDFHSRTAMNMYPHIR AVE G
Sbjct: 788 FIASPGNSLIVADYGQLELRILAHLASCESMKEAFIAGGDFHSRTAMNMYPHIREAVENG 847
Query: 929 SVLLEWDPQPGEDKPPVPLLKDAFGSERRKAKMLNFSIAYGKTPVGLSKDWKVTVEEARQ 988
VLLEW PQPG++KPPVPLLKDAF SERRKAKMLNFSIAYGKT +GLS+DWKV+ EEA+
Sbjct: 848 EVLLEWHPQPGQEKPPVPLLKDAFASERRKAKMLNFSIAYGKTAIGLSRDWKVSREEAQD 907
Query: 989 TVDLWYNERKEVRIWQELRKKEADEKSCVRTLLGRARRFPSMKHATRAQRGHIERAAINT 1048
TV+LWYN+R+EVR WQELRKKEA +K V TLLGRAR+FP ++ +RAQ+ HIERAAINT
Sbjct: 908 TVNLWYNDRQEVRKWQELRKKEAIQKGYVLTLLGRARKFP--EYRSRAQKNHIERAAINT 967
Query: 1049 PVQGSAADVAMCAMLEISNNSGLRELGWRLLLQVHDEVILEGPTESAEVAKAIVVECMSK 1108
PVQGSAADVAMCAMLEISNN L+ELGW+LLLQVHDEVILEGP+ESAE AK IVV CMS+
Sbjct: 968 PVQGSAADVAMCAMLEISNNQRLKELGWKLLLQVHDEVILEGPSESAENAKDIVVNCMSE 1027
Query: 1109 PFSGKNILNVDLAVDAKCAQNWYSAK 1133
PF+GKNIL+VDL+VDAKCAQNWY+ K
Sbjct: 1028 PFNGKNILSVDLSVDAKCAQNWYAGK 1049
BLAST of MC10g1035 vs. TAIR 10
Match:
AT4G32700.2 (helicases;ATP-dependent helicases;nucleic acid binding;ATP binding;DNA-directed DNA polymerases;DNA binding )
HSP 1 Score: 92.0 bits (227), Expect = 3.2e-18
Identity = 90/332 (27.11%), Postives = 140/332 (42.17%), Query Frame = 0
Query: 834 LNINTETGRLSARRPNLQN--------------QPALEKDRYKI--RQAFIAAPGN-SLI 893
L +T TGRLS PNLQ+ + + DRYKI R F+ N L+
Sbjct: 1846 LQTSTATGRLSIEEPNLQSVEHEVEFKLDKNGRDVSSDADRYKINARDFFVPTQENWLLL 1905
Query: 894 VADYGQLELRILAHLANCKSMLEAF-KAGGDFHSRTAMNMYPHIRNAVEKGSVLLEWDPQ 953
ADY Q+ELR++AH + S++ + GD + A +W +
Sbjct: 1906 TADYSQIELRLMAHFSRDSSLISKLSQPEGDVFTMIA-----------------AKWTGK 1965
Query: 954 PGEDKPPVPLLKDAFGSERRKAKMLNFSIAYGKTPVGLSKDWKVTVEEARQTVDLWYNER 1013
+ P +R + K L + I YG L++ + T +EA++ + + +
Sbjct: 1966 AEDSVSP---------HDRDQTKRLIYGILYGMGANRLAEQLECTSDEAKEKIRSFKSSF 2025
Query: 1014 KEVRIWQELRKKEADEKSCVRTLLGRARRFPSMKHATRAQRGHIERAAINTPVQGSAADV 1073
V W EK ++TL GR R +K ++ +R A+N+ QGSAAD+
Sbjct: 2026 PAVTSWLNETISFCQEKGYIQTLKGRRRFLSKIKFGNAKEKSKAQRQAVNSMCQGSAADI 2085
Query: 1074 AMCAMLEI-------------SNNSGLR----ELGWRLLLQVHDEVILEGPTESAEVAKA 1131
AM+ I S++S R + R+LLQVHDE++LE ++A
Sbjct: 2086 IKIAMINIYSAIAEDVDTAASSSSSETRFHMLKGRCRILLQVHDELVLEVDPSYVKLAAM 2145
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
F4I6M1 | 0.0e+00 | 69.87 | DNA polymerase I A, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 ... | [more] |
Q84ND9 | 0.0e+00 | 72.58 | DNA polymerase I B, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 ... | [more] |
Q6Z4T5 | 1.1e-312 | 64.75 | DNA polymerase I A, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os... | [more] |
Q6Z4T3 | 1.5e-307 | 65.67 | DNA polymerase I B, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os... | [more] |
Q9CDS1 | 1.6e-43 | 33.55 | DNA polymerase I OS=Lactococcus lactis subsp. lactis (strain IL1403) OX=272623 G... | [more] |
Match Name | E-value | Identity | Description | |
XP_022150985.1 | 0.0 | 100.00 | DNA polymerase I A, chloroplastic/mitochondrial [Momordica charantia] | [more] |
KAG7015499.1 | 0.0 | 83.16 | DNA polymerase I A, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma... | [more] |
KAG7031413.1 | 0.0 | 83.41 | DNA polymerase I A, chloroplastic/mitochondrial [Cucurbita argyrosperma subsp. a... | [more] |
XP_023536872.1 | 0.0 | 83.32 | DNA polymerase I B, chloroplastic/mitochondrial-like [Cucurbita pepo subsp. pepo... | [more] |
KAG6600776.1 | 0.0 | 83.30 | DNA polymerase I A, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1D9Z5 | 0.0 | 100.00 | DNA polymerase I A, chloroplastic/mitochondrial OS=Momordica charantia OX=3673 G... | [more] |
A0A6J1FW48 | 0.0 | 83.23 | DNA polymerase I B, chloroplastic/mitochondrial-like OS=Cucurbita moschata OX=36... | [more] |
A0A6J1IIP1 | 0.0 | 83.22 | DNA polymerase I A, chloroplastic/mitochondrial-like OS=Cucurbita maxima OX=3661... | [more] |
A0A6J1EVX1 | 0.0 | 80.96 | DNA polymerase I A, chloroplastic/mitochondrial-like OS=Cucurbita moschata OX=36... | [more] |
A0A6J1J7E3 | 0.0 | 81.58 | DNA polymerase I A, chloroplastic/mitochondrial isoform X1 OS=Cucurbita maxima O... | [more] |