MC10g0603 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC10g0603
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionprotein HEAT INTOLERANT 4-like
LocationMC10: 4979027 .. 4984040 (-)
RNA-Seq ExpressionMC10g0603
SyntenyMC10g0603
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
TTAAGGAATTTTATTAGACACGCTATTAAGTTATTAAATACTTTTTCTACTTAAAAAAAATTATTATTCTTAAACATCTAGACTAAAATACGTAATTAAAAAAAGACTTTTCGAAATGTAAAGGGAAAATAGAAAATTAGTAATAACTGAAGAAAATAACCAAAAAAAAAAAAAAAATCGCCTCCTCTTCTGGGAGAAAGAAGACATGCGTGTGACTCTGTCGGGCTGTGTGTGAAAAAATTGAAAATTTCAACAAATTTTGAAATAATTTCGATTTCAACATTTTTAGACGTCCCGCCTCCGTGAAGCTGAGGAAAAGGAGCAGCCGCGTTGATCTTACGCTCGATAATCCCCAACCCCAAAACCCTATTTCTCTCTCTCTTATCCTCTCGATCTTTCTACCTCTACATTTGCAATTTATTCGAATCCGGATCCTTCGGTTTCCATCGCAGCAGCCGATTTCTCCTTCTTCTTCGCGCTCTATTCCCCGTTTTCCAGCTGAGCTGCGGCCACAATGAGGAAAGGTACTAAGAGGAAGACAGCTCGAAAAGAAGAAGATAAACCTGTAGAACCTAAGCGCAAAGAAGCTCCGTCTCGAGCCAAGCGGGCCAAGCTCCCCAAACCCGAATCCGAACCCGAGTACTTTGAGGACAAGCGTAACTTGGTATTGTATTTTGTGCCACACCCCTTTTTGTTGCTGTTTTAAACTTTGATTGTCGTGCGCTACTATGGGATTTCTTGCGTCACTTTGCTGGGTCTTGAATTTCCACTGTCGTGAAGTATTCTGAAAAATTGACGTTGGTAATTGATCCTTTTCGGTGCCGAATTTTGTGGATGGTTTCATTTGCTCTTGTGGGTTTCTTGAATAATTAACTTGCGCTTGTAAATTAAGCTCGGTTTTACTTTTGGCAAGCTGTTGGGCTGGAATCAGATGCTTGTTTTTGGTAATTTGATGTTACCTGCAATGGGGTTCTTTTTAGAAATTCTAAGAGTGTATGTAGATTGCGAATTTGTGTTTTCTTGTTTCTTTTCATTTGCGAATGACATCGTAAAATGCAGGAAGATCTGTGGAAAGCAGCATTTCCTGTTGGGACAGAGGTTTGGCATAAACTATGGTACTTGTTTCTTTTTTAACAGAAGTCTTATTGTTGTGTTGCTTATGCATTCACATGTTTTTCTATGTCAGTGGGATCAATTGGACACGGTTTATCAATTCAACTGGAATTTCTCAAATCTAGAAGTGAGTTCAAATAATATAATCTGTCAAAGTCTAATATCAGTTGGTTTTTCGACTTATATTGGATGCATTAGAGTTAAATTTACTGGGGATATAACATTTGTCATTATTGTCAGGATGCATTTGAAGAGGGGGGAAAGTTGTATGGTGAGAAGGTTTATCTATTTGGTTGTACAGAGCGTAAGTAGCTAACTAAGAATCTTGTTTTACATTTTCAAACTTATACAAGACAAATTTATTGAGGTCCAGTATATTGTTTCAACCTCCGTCAAAAGGAAGGCAAGCCTGAAAGAACTTTTCTTTCACCTTACCTTATTTTTTTAGTCTATTTATGTTAATTCATGTTTGTGCAGCTCAATTGGTTCCTTTCAAGGGTGAAAATAAAGTTATATGCATACCTGCTGTGGTGGCTGTAAGTTACATTTCTTTTAGTTTTCGTATATCGTGTTGATTGTTTTTGTTCTATACAAATACAAAGGCTTCTTTATATAACCAATTAGTGGGTTCTTATCCAGATGCTAAGACAACCTTAAAACTTTTTGCTTTTAAAATTTTATTTTTCATTGCTCATGGTGTTTATGTTATGATTTTTTTCTTTTTCTTTATGTAATACCACTGGCCTTTTGGATTCTCTCGTGTCAATCTGCGGCTATTTTTAGCCTTGTGAATTGACTGACTGCCATATAACAAATTTCAAATTGAAAGAAGTTATCGGCTAATTATTACTTTTCTCATTTGTTGACAAGAACATGTGGAGTATACACTTTAAGTTTTAACATATTGCTTCCGAATCTTTCTGCCTCTTCAAACTTCAAAGTGGATATTTCCTTTGAGTATATACTTTCTAAAAGTCTGGATGTTTAACTTAATTTTCTAAAATAATATTTTACCCAATTCACGGGATTTAATTCCTATGACTATTGGTTTTTGCGGCTCTATAGGTTGTATCTCCTTTCCCACCTTCTGATAAAATTGGAATAAACTCTGTTCAAAGGGAGGCTGAAGAGATAGTTCCCATGAAACAGATGAAGATGGACTGGGTTCCTTATATTCCTTTGGAGAAAAGGTCTTAATTTGTGAATTTTTCTTAGCCTCTTTTATATTCTAACTCTAGTTTTTTAATTGACTATGCAATCTAATTCTTCTTCAGAGAGAGCCGAGTTGATAAGTTGAAATCTCAAATATTCATTCTCAGTTGCACTCAGAGAAGGTACAAATATGAGTTGCCTTTCGCTGTTGTGTCACAGCAGTTAATTTCACATTTAATCTATTCATTTTTAAAATGCCAGTCTTCAACAAAAGTGTGTTTATTGCTAACTTAAAATTTTTTAATTTAATGACACGTCATGCGTGATGCTCATTCAAATTGTTTCTGTAGAGCTGCTTTGAAGCATTTGAAAATAGATCGTGTCAAGAAATATGAGTATTGCTTGCCCTGTAAGTTCTTCTATCTATTTGGTTGTTCTTAAAAATTTTATGATTGAATTTATTATATGAGAAGAGGAGCTGTGAGTTCTTGGGAGGAGCAAGTGAAATATCTTGAGAGTGAAACTGATCTCTAGTTGTAGTTTTATACCCATGACACGTTCAATTCTACTTAGTTATAATTTCTTGATTTTTAACGCCATCTAGAAATCCAGTTAATGATTATCGATTTATTTTATACCAGTCTGTCTTATCTTATATGTTGTTATTTTTCTGATAGATTTAAGGAAGATGAATTTAAACAAAACTAAAGTAATTTCTAGTTGTGGTTTTCTGCCTATGACACTTTCAATTTTACTTTATATAATTTCTTCATTTTAATGTCATCTAGAAACCCAGCTCATGGTTATCATTAAATATTATGCACGTAGTTTCCCGCCCTTGCAGTTCCAATTATACTTAATGTGATTTCTTGAATTGTTAATGTCAACTAGATGATCCAGTTAATGATTATTGTTCAATTTTATACAAGTCTGTCTTATCTTATATGCTGTTATTTTTCTGATAGATTTCTACCAGCCTTTTAAGGAAGATGAATTTGAACAAAGCACTGAGGTTCCAATTATTTTTCCGATTGACCCAAAGCCAGTAAGGTTTTAATCCATGCTATTTAAAAATGCTCCTAAATGCATAGTGTGTAGGGAAGAGATTAGTTGAGCATTGTTATTCACAATCTGAAATTTATATGCTTATATTTTCAGATTTTGATGCCCATTTGGATTAGGATGATCATGCAGGGGAACTGCAAATCTATATGTAAGTTTAACTTTTTTGTTATCTGCAGGTTTTCTGTGAGTTTGATTGGGAATTAGATGAACTCGAGGTACAAGATGTTAATTCAAATGGCTGTCTTGATTATTATCTTTGAATAATTTAGGCTGCTGGTCGTTTATGAAATTTTTCTTTAAAGGGAGATTTCCCTAGAGTCTTATACGCTGTTTTCAAATTTCCTCAGGAGTTCACAGATAAGTTGATCGAGGAGGAAGAATTATCTGAAAGTCAAAAGGACGCCTTCAAGGTAGTTCATCTAGTATTGCAGCCAAGTTTCAGCTATTTATCTTTATACAATCTTACCTGATGATGGTTCTCGACTTTTTAGGATTTTGTAAAGGAGAAAGTTAGAGAGGCCAAAAAAGCCAATAGAGAGGTAAATGTTGTTAGTCATTTTGATTTGTTATGAGCAAGTTTAGCGAGTAACAATTACATGCGGATCTTATTCAATTTTCTTTTTCAGGCTAGGGAAGCACGGAAAAAGGCCATTGAGGAAATGAGCAAAGAAACTAAAGAAGCGTTTGAGAAGATGAAATTTTACAAGTTTTACCCTGTACAAACGCCTGATACACCCGATATATCTAATGTTAAGGTAAGATTGAACCTGCTTTCATTCTCTTGGCCAATGATGAGTGGAACATGTTGAACTTGAACTGCATCTTTGATAAATCTCATTCTTATTCATCAACAATGGGTAGAAGCTATAGGAAGACTTTAGAGAGCTTCCACCTCTCTCCAGCCAAACTAATAGTAGACGACCAAAATCCTATGCCCCCATCAGCTAACATTCTATAAATACTCCCCATAGTTCACTCATCAGTTACATCATAAGCGTATTGTTATAAAGTTGGTACCTTTTAACCAACTCGTAATCTAAGTGCTGCCCCCTCCTCTTGGCTCATTTACTCGTACACATTTACAATTGGAGTCTTATCAATCTTAAAATTATTTTCTCTCACATTATTTTTGCCTGCATTTCTGTAGTGTCTAATAATTTTGGCCTTCCAGGCACCATTTATAAATAGGTACTACGGAAAGGCTCATGAAGTTCTGTGATCTTATACCATGGCTGGCACAAGATAGAAATTGATATAAAGCTCCTTCAGGTTCAGATTTTGTTCAACGTTTAAAGGTACTGTTCCCATTATGCTGAAATTTGTGATGCCAATGCCTTCCAACTTATTACATCAATCTAGCGATGACGGGATCTTGAACAGTAGCCCTCCGAAATCCTTAGATAGACTGCTGTAATATTTAGGATCATGCTTTCTTTCTTTGATCGTTCTTTCTTTTTTGAGATTTCTTCTTTCCGTCAGTAAAAAAGGACTTTAGAACCTCACTGACCTCTAGTTGGAGTATATTGGTGTAATTCTGTTCATTATTCCATCCATCACTTCAGTTTTGTATAACCATAGTTTCAGTTCTACATCCTCGAGAACAAATTATGTTACCTGCTTACTGCACTTGAAATGCCATTACACATTTTTACAGTATTTGAAGGATATTTGTTTTATTCCATTT

mRNA sequence

TTAAGGAATTTTATTAGACACGCTATTAAGTTATTAAATACTTTTTCTACTTAAAAAAAATTATTATTCTTAAACATCTAGACTAAAATACGTAATTAAAAAAAGACTTTTCGAAATGTAAAGGGAAAATAGAAAATTAGTAATAACTGAAGAAAATAACCAAAAAAAAAAAAAAAATCGCCTCCTCTTCTGGGAGAAAGAAGACATGCGTGTGACTCTGTCGGGCTGTGTGTGAAAAAATTGAAAATTTCAACAAATTTTGAAATAATTTCGATTTCAACATTTTTAGACGTCCCGCCTCCGTGAAGCTGAGGAAAAGGAGCAGCCGCGTTGATCTTACGCTCGATAATCCCCAACCCCAAAACCCTATTTCTCTCTCTCTTATCCTCTCGATCTTTCTACCTCTACATTTGCAATTTATTCGAATCCGGATCCTTCGGTTTCCATCGCAGCAGCCGATTTCTCCTTCTTCTTCGCGCTCTATTCCCCGTTTTCCAGCTGAGCTGCGGCCACAATGAGGAAAGGTACTAAGAGGAAGACAGCTCGAAAAGAAGAAGATAAACCTGTAGAACCTAAGCGCAAAGAAGCTCCGTCTCGAGCCAAGCGGGCCAAGCTCCCCAAACCCGAATCCGAACCCGAGTACTTTGAGGACAAGCGTAACTTGGAAGATCTGTGGAAAGCAGCATTTCCTGTTGGGACAGAGTGGGATCAATTGGACACGGTTTATCAATTCAACTGGAATTTCTCAAATCTAGAAGATGCATTTGAAGAGGGGGGAAAGTTGTATGGTGAGAAGGTTTATCTATTTGGTTGTACAGAGCCTCAATTGGTTCCTTTCAAGGGTGAAAATAAAGTTATATGCATACCTGCTGTGGTGGCTGTTGTATCTCCTTTCCCACCTTCTGATAAAATTGGAATAAACTCTGTTCAAAGGGAGGCTGAAGAGATAGTTCCCATGAAACAGATGAAGATGGACTGGGTTCCTTATATTCCTTTGGAGAAAAGAGAGAGCCGAGTTGATAAGTTGAAATCTCAAATATTCATTCTCAGTTGCACTCAGAGAAGAGCTGCTTTGAAGCATTTGAAAATAGATCGTGTCAAGAAATATGAGTATTGCTTGCCCTATTTCTACCAGCCTTTTAAGGAAGATGAATTTGAACAAAGCACTGAGGTTCCAATTATTTTTCCGATTGACCCAAAGCCAGTTTTCTGTGAGTTTGATTGGGAATTAGATGAACTCGAGGAGTTCACAGATAAGTTGATCGAGGAGGAAGAATTATCTGAAAGTCAAAAGGACGCCTTCAAGGATTTTGTAAAGGAGAAAGTTAGAGAGGCCAAAAAAGCCAATAGAGAGGCTAGGGAAGCACGGAAAAAGGCCATTGAGGAAATGAGCAAAGAAACTAAAGAAGCGTTTGAGAAGATGAAATTTTACAAGTTTTACCCTGTACAAACGCCTGATACACCCGATATATCTAATGTTAAGGCACCATTTATAAATAGGTACTACGGAAAGGCTCATGAAGTTCTGTGATCTTATACCATGGCTGGCACAAGATAGAAATTGATATAAAGCTCCTTCAGGTTCAGATTTTGTTCAACGTTTAAAGGTACTGTTCCCATTATGCTGAAATTTGTGATGCCAATGCCTTCCAACTTATTACATCAATCTAGCGATGACGGGATCTTGAACAGTAGCCCTCCGAAATCCTTAGATAGACTGCTGTAATATTTAGGATCATGCTTTCTTTCTTTGATCGTTCTTTCTTTTTTGAGATTTCTTCTTTCCGTCAGTAAAAAAGGACTTTAGAACCTCACTGACCTCTAGTTGGAGTATATTGGTGTAATTCTGTTCATTATTCCATCCATCACTTCAGTTTTGTATAACCATAGTTTCAGTTCTACATCCTCGAGAACAAATTATGTTACCTGCTTACTGCACTTGAAATGCCATTACACATTTTTACAGTATTTGAAGGATATTTGTTTTATTCCATTT

Coding sequence (CDS)

ATGAGGAAAGGTACTAAGAGGAAGACAGCTCGAAAAGAAGAAGATAAACCTGTAGAACCTAAGCGCAAAGAAGCTCCGTCTCGAGCCAAGCGGGCCAAGCTCCCCAAACCCGAATCCGAACCCGAGTACTTTGAGGACAAGCGTAACTTGGAAGATCTGTGGAAAGCAGCATTTCCTGTTGGGACAGAGTGGGATCAATTGGACACGGTTTATCAATTCAACTGGAATTTCTCAAATCTAGAAGATGCATTTGAAGAGGGGGGAAAGTTGTATGGTGAGAAGGTTTATCTATTTGGTTGTACAGAGCCTCAATTGGTTCCTTTCAAGGGTGAAAATAAAGTTATATGCATACCTGCTGTGGTGGCTGTTGTATCTCCTTTCCCACCTTCTGATAAAATTGGAATAAACTCTGTTCAAAGGGAGGCTGAAGAGATAGTTCCCATGAAACAGATGAAGATGGACTGGGTTCCTTATATTCCTTTGGAGAAAAGAGAGAGCCGAGTTGATAAGTTGAAATCTCAAATATTCATTCTCAGTTGCACTCAGAGAAGAGCTGCTTTGAAGCATTTGAAAATAGATCGTGTCAAGAAATATGAGTATTGCTTGCCCTATTTCTACCAGCCTTTTAAGGAAGATGAATTTGAACAAAGCACTGAGGTTCCAATTATTTTTCCGATTGACCCAAAGCCAGTTTTCTGTGAGTTTGATTGGGAATTAGATGAACTCGAGGAGTTCACAGATAAGTTGATCGAGGAGGAAGAATTATCTGAAAGTCAAAAGGACGCCTTCAAGGATTTTGTAAAGGAGAAAGTTAGAGAGGCCAAAAAAGCCAATAGAGAGGCTAGGGAAGCACGGAAAAAGGCCATTGAGGAAATGAGCAAAGAAACTAAAGAAGCGTTTGAGAAGATGAAATTTTACAAGTTTTACCCTGTACAAACGCCTGATACACCCGATATATCTAATGTTAAGGCACCATTTATAAATAGGTACTACGGAAAGGCTCATGAAGTTCTGTGA

Protein sequence

MRKGTKRKTARKEEDKPVEPKRKEAPSRAKRAKLPKPESEPEYFEDKRNLEDLWKAAFPVGTEWDQLDTVYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENKVICIPAVVAVVSPFPPSDKIGINSVQREAEEIVPMKQMKMDWVPYIPLEKRESRVDKLKSQIFILSCTQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDEFEQSTEVPIIFPIDPKPVFCEFDWELDELEEFTDKLIEEEELSESQKDAFKDFVKEKVREAKKANREAREARKKAIEEMSKETKEAFEKMKFYKFYPVQTPDTPDISNVKAPFINRYYGKAHEVL
Homology
BLAST of MC10g0603 vs. ExPASy Swiss-Prot
Match: A2RVJ8 (Protein HEAT INTOLERANT 4 OS=Arabidopsis thaliana OX=3702 GN=HIT4 PE=1 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 4.6e-135
Identity = 236/337 (70.03%), Postives = 289/337 (85.76%), Query Frame = 0

Query: 2   RKGTKRKTARKEEDKPVEPKRKEAPSRAKRAKLPKPESEPEYFEDKRNLEDLWKAAFPVG 61
           R+G KRK A K +D  ++ ++K  P +AK+ +  K + EP YFE+KR+LEDLWK AFPVG
Sbjct: 101 RRGGKRKRATK-KDTEIKDEKKPVP-KAKKPRAAKVKEEPVYFEEKRSLEDLWKVAFPVG 160

Query: 62  TEWDQLDTVYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENKVICIPAVV 121
           TEWDQLD +Y+FNW+F NLE+A EEGGKLYG+KVY+FGCTEPQLVP+KG NK++ +PAVV
Sbjct: 161 TEWDQLDALYEFNWDFQNLEEALEEGGKLYGKKVYVFGCTEPQLVPYKGANKIVHVPAVV 220

Query: 122 AVVSPFPPSDKIGINSVQREAEEIVPMKQMKMDWVPYIPLEKRESRVDKLKSQIFILSCT 181
            + SPFPPSDKIGI SVQRE EEI+PMK+MKMDW+PYIP+EKR+ +VDK+ SQIF L CT
Sbjct: 221 VIESPFPPSDKIGITSVQREVEEIIPMKKMKMDWLPYIPIEKRDRQVDKMNSQIFTLGCT 280

Query: 182 QRRAALKHLKIDRVKKYEYCLPYFYQPFKEDEFEQSTEVPIIFPIDPKPVFCEFDWELDE 241
           QRR+AL+H+K D++KK+EYCLPYFYQPFKEDE EQSTEV I+FP +P PV CEFDWE DE
Sbjct: 281 QRRSALRHMKEDQLKKFEYCLPYFYQPFKEDELEQSTEVQIMFPSEP-PVVCEFDWEFDE 340

Query: 242 LEEFTDKLIEEEELSESQKDAFKDFVKEKVREAKKANREAREARKKAIEEMSKETKEAFE 301
           L+EF DKL+EEE L   Q D FK++VKE+VR AKKANREA++ARKKAIEEMS++TK+AF+
Sbjct: 341 LQEFVDKLVEEEALPAEQADEFKEYVKEQVRAAKKANREAKDARKKAIEEMSEDTKQAFQ 400

Query: 302 KMKFYKFYPVQTPDTPDISNVKAPFINRYYGKAHEVL 339
           KMKFYKFYP  +PDTPD+S V++PFINRYYGKAHEVL
Sbjct: 401 KMKFYKFYPQPSPDTPDVSGVQSPFINRYYGKAHEVL 434

BLAST of MC10g0603 vs. NCBI nr
Match: XP_022136810.1 (uncharacterized protein LOC111008415 [Momordica charantia])

HSP 1 Score: 666 bits (1718), Expect = 3.57e-241
Identity = 338/338 (100.00%), Postives = 338/338 (100.00%), Query Frame = 0

Query: 1   MRKGTKRKTARKEEDKPVEPKRKEAPSRAKRAKLPKPESEPEYFEDKRNLEDLWKAAFPV 60
           MRKGTKRKTARKEEDKPVEPKRKEAPSRAKRAKLPKPESEPEYFEDKRNLEDLWKAAFPV
Sbjct: 1   MRKGTKRKTARKEEDKPVEPKRKEAPSRAKRAKLPKPESEPEYFEDKRNLEDLWKAAFPV 60

Query: 61  GTEWDQLDTVYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENKVICIPAV 120
           GTEWDQLDTVYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENKVICIPAV
Sbjct: 61  GTEWDQLDTVYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENKVICIPAV 120

Query: 121 VAVVSPFPPSDKIGINSVQREAEEIVPMKQMKMDWVPYIPLEKRESRVDKLKSQIFILSC 180
           VAVVSPFPPSDKIGINSVQREAEEIVPMKQMKMDWVPYIPLEKRESRVDKLKSQIFILSC
Sbjct: 121 VAVVSPFPPSDKIGINSVQREAEEIVPMKQMKMDWVPYIPLEKRESRVDKLKSQIFILSC 180

Query: 181 TQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDEFEQSTEVPIIFPIDPKPVFCEFDWELD 240
           TQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDEFEQSTEVPIIFPIDPKPVFCEFDWELD
Sbjct: 181 TQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDEFEQSTEVPIIFPIDPKPVFCEFDWELD 240

Query: 241 ELEEFTDKLIEEEELSESQKDAFKDFVKEKVREAKKANREAREARKKAIEEMSKETKEAF 300
           ELEEFTDKLIEEEELSESQKDAFKDFVKEKVREAKKANREAREARKKAIEEMSKETKEAF
Sbjct: 241 ELEEFTDKLIEEEELSESQKDAFKDFVKEKVREAKKANREAREARKKAIEEMSKETKEAF 300

Query: 301 EKMKFYKFYPVQTPDTPDISNVKAPFINRYYGKAHEVL 338
           EKMKFYKFYPVQTPDTPDISNVKAPFINRYYGKAHEVL
Sbjct: 301 EKMKFYKFYPVQTPDTPDISNVKAPFINRYYGKAHEVL 338

BLAST of MC10g0603 vs. NCBI nr
Match: XP_038878042.1 (protein HEAT INTOLERANT 4-like [Benincasa hispida])

HSP 1 Score: 638 bits (1646), Expect = 3.36e-230
Identity = 321/338 (94.97%), Postives = 327/338 (96.75%), Query Frame = 0

Query: 1   MRKGTKRKTARKEEDKPVEPKRKEAPSRAKRAKLPKPESEPEYFEDKRNLEDLWKAAFPV 60
           MRKGTKRK  RKE+DKP EPK KEAPSRAKR KLPKPESEPEYFEDKRNLEDLWKAAFPV
Sbjct: 1   MRKGTKRKATRKEQDKPAEPKPKEAPSRAKRTKLPKPESEPEYFEDKRNLEDLWKAAFPV 60

Query: 61  GTEWDQLDTVYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENKVICIPAV 120
           GTEWDQLD+VYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENKVICIP V
Sbjct: 61  GTEWDQLDSVYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENKVICIPVV 120

Query: 121 VAVVSPFPPSDKIGINSVQREAEEIVPMKQMKMDWVPYIPLEKRESRVDKLKSQIFILSC 180
           VAV SPFPPSDKIGINSVQREAEEIVPMKQMKMDWVPYIPLEKR+SRVDKLKSQIFILSC
Sbjct: 121 VAVASPFPPSDKIGINSVQREAEEIVPMKQMKMDWVPYIPLEKRDSRVDKLKSQIFILSC 180

Query: 181 TQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDEFEQSTEVPIIFPIDPKPVFCEFDWELD 240
           TQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDEFEQSTEVPIIFP DPKPVFCEFDW  D
Sbjct: 181 TQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDEFEQSTEVPIIFPADPKPVFCEFDWGFD 240

Query: 241 ELEEFTDKLIEEEELSESQKDAFKDFVKEKVREAKKANREAREARKKAIEEMSKETKEAF 300
           ELEEFTDKLIE+EELSE+QKDAFKDFV+EKVREAKKANREAREAR KAIEEMSKETKEAF
Sbjct: 241 ELEEFTDKLIEDEELSENQKDAFKDFVREKVREAKKANREAREARNKAIEEMSKETKEAF 300

Query: 301 EKMKFYKFYPVQTPDTPDISNVKAPFINRYYGKAHEVL 338
           EKMKFYKFYPVQTPDTPDISNVKAPFINRYYGKAHEVL
Sbjct: 301 EKMKFYKFYPVQTPDTPDISNVKAPFINRYYGKAHEVL 338

BLAST of MC10g0603 vs. NCBI nr
Match: XP_004149148.1 (protein HEAT INTOLERANT 4-like [Cucumis sativus] >XP_031740732.1 protein HEAT INTOLERANT 4 [Cucumis sativus] >KAE8637581.1 hypothetical protein CSA_017858 [Cucumis sativus])

HSP 1 Score: 637 bits (1642), Expect = 1.37e-229
Identity = 318/338 (94.08%), Postives = 328/338 (97.04%), Query Frame = 0

Query: 1   MRKGTKRKTARKEEDKPVEPKRKEAPSRAKRAKLPKPESEPEYFEDKRNLEDLWKAAFPV 60
           MRKGTKRK ARKEEDKP EPK  +APSRAKR KLPKPESEPEYFEDKRN+EDLWKAAFPV
Sbjct: 1   MRKGTKRKAARKEEDKPAEPKPDKAPSRAKRTKLPKPESEPEYFEDKRNMEDLWKAAFPV 60

Query: 61  GTEWDQLDTVYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENKVICIPAV 120
           GTEWDQLD+VYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENKVICIP V
Sbjct: 61  GTEWDQLDSVYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENKVICIPVV 120

Query: 121 VAVVSPFPPSDKIGINSVQREAEEIVPMKQMKMDWVPYIPLEKRESRVDKLKSQIFILSC 180
           VAV SPFPPSDKIGINSVQREAEEI+PMKQMKMDWVPYIPLEKR+ RVDKLKSQIFILSC
Sbjct: 121 VAVASPFPPSDKIGINSVQREAEEIIPMKQMKMDWVPYIPLEKRDRRVDKLKSQIFILSC 180

Query: 181 TQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDEFEQSTEVPIIFPIDPKPVFCEFDWELD 240
           TQRRAALKHLKIDR+KKYEYCLPYFYQPFK+DEFEQSTEVPIIFP+DPKPVFCEFDWE D
Sbjct: 181 TQRRAALKHLKIDRLKKYEYCLPYFYQPFKDDEFEQSTEVPIIFPVDPKPVFCEFDWEFD 240

Query: 241 ELEEFTDKLIEEEELSESQKDAFKDFVKEKVREAKKANREAREARKKAIEEMSKETKEAF 300
           ELEEFTDKLIEEEELSESQKDAFKDFV+EKVREAKKANREAREARKKAIEEMS ETKEAF
Sbjct: 241 ELEEFTDKLIEEEELSESQKDAFKDFVREKVREAKKANREAREARKKAIEEMSNETKEAF 300

Query: 301 EKMKFYKFYPVQTPDTPDISNVKAPFINRYYGKAHEVL 338
           EKMKFYKFYPVQTPD+PDISNVKAPFINRYYGKAHEVL
Sbjct: 301 EKMKFYKFYPVQTPDSPDISNVKAPFINRYYGKAHEVL 338

BLAST of MC10g0603 vs. NCBI nr
Match: XP_008453747.1 (PREDICTED: uncharacterized protein LOC103494391 [Cucumis melo])

HSP 1 Score: 625 bits (1613), Expect = 3.60e-225
Identity = 313/338 (92.60%), Postives = 324/338 (95.86%), Query Frame = 0

Query: 1   MRKGTKRKTARKEEDKPVEPKRKEAPSRAKRAKLPKPESEPEYFEDKRNLEDLWKAAFPV 60
           MRKGTKRK  RKEEDKP EPK +++P RAKR KLPKPESEPEYFEDKRN+EDLWKAAFPV
Sbjct: 1   MRKGTKRKATRKEEDKPAEPKPEKSPPRAKRTKLPKPESEPEYFEDKRNMEDLWKAAFPV 60

Query: 61  GTEWDQLDTVYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENKVICIPAV 120
           GTEWDQLD+VY+FNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENK+  IP V
Sbjct: 61  GTEWDQLDSVYKFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENKLTIIPVV 120

Query: 121 VAVVSPFPPSDKIGINSVQREAEEIVPMKQMKMDWVPYIPLEKRESRVDKLKSQIFILSC 180
           VAV SPFPPSDKIGINSVQREAEEIVPMKQMKMDWVPYIPLEKR+ RVDKLKSQIFILSC
Sbjct: 121 VAVASPFPPSDKIGINSVQREAEEIVPMKQMKMDWVPYIPLEKRDRRVDKLKSQIFILSC 180

Query: 181 TQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDEFEQSTEVPIIFPIDPKPVFCEFDWELD 240
           TQRRAALKHLKIDRVKKYEYCLPYFYQPFK+DEFEQSTEVPIIFP+DPKPVFCEFDWELD
Sbjct: 181 TQRRAALKHLKIDRVKKYEYCLPYFYQPFKDDEFEQSTEVPIIFPVDPKPVFCEFDWELD 240

Query: 241 ELEEFTDKLIEEEELSESQKDAFKDFVKEKVREAKKANREAREARKKAIEEMSKETKEAF 300
           ELEEFTDKLIE EELSESQKDAFKDFV+EKVRE KKANREAREARKKAIEEMS ETKEAF
Sbjct: 241 ELEEFTDKLIENEELSESQKDAFKDFVREKVRETKKANREAREARKKAIEEMSNETKEAF 300

Query: 301 EKMKFYKFYPVQTPDTPDISNVKAPFINRYYGKAHEVL 338
           EKMKFYKFYPVQTPDTPDISNVKAPFINRYYGKAHEVL
Sbjct: 301 EKMKFYKFYPVQTPDTPDISNVKAPFINRYYGKAHEVL 338

BLAST of MC10g0603 vs. NCBI nr
Match: XP_022981812.1 (protein HEAT INTOLERANT 4-like [Cucurbita maxima])

HSP 1 Score: 623 bits (1606), Expect = 4.20e-224
Identity = 315/338 (93.20%), Postives = 324/338 (95.86%), Query Frame = 0

Query: 1   MRKGTKRKTARKEEDKPVEPKRKEAPSRAKRAKLPKPESEPEYFEDKRNLEDLWKAAFPV 60
           MRKGTKRK  RKE+ KP +PK KE PSR KR K+PKPESEPEYFED+RNLEDLWKAAFPV
Sbjct: 1   MRKGTKRKATRKEDHKPAQPKPKEVPSRTKRIKVPKPESEPEYFEDQRNLEDLWKAAFPV 60

Query: 61  GTEWDQLDTVYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENKVICIPAV 120
           GTEWDQLD+VYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVP+KGENKV  IPAV
Sbjct: 61  GTEWDQLDSVYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPYKGENKVTVIPAV 120

Query: 121 VAVVSPFPPSDKIGINSVQREAEEIVPMKQMKMDWVPYIPLEKRESRVDKLKSQIFILSC 180
           VAVVSPFPPSDKIGINSVQREAEEIVPMKQMKMDWVPYIPLEKR+S+VDKLKSQIFILSC
Sbjct: 121 VAVVSPFPPSDKIGINSVQREAEEIVPMKQMKMDWVPYIPLEKRDSQVDKLKSQIFILSC 180

Query: 181 TQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDEFEQSTEVPIIFPIDPKPVFCEFDWELD 240
           TQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDE EQSTEVPIIFPIDPKPVFCEFDWELD
Sbjct: 181 TQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDEIEQSTEVPIIFPIDPKPVFCEFDWELD 240

Query: 241 ELEEFTDKLIEEEELSESQKDAFKDFVKEKVREAKKANREAREARKKAIEEMSKETKEAF 300
           ELEEFTDKLIEEEEL E+QKDAFK FVKEKVREAKKANREAREARKKAIEEMSKETKEAF
Sbjct: 241 ELEEFTDKLIEEEELPENQKDAFKAFVKEKVREAKKANREAREARKKAIEEMSKETKEAF 300

Query: 301 EKMKFYKFYPVQTPDTPDISNVKAPFINRYYGKAHEVL 338
           EKMKFYKFYPVQT DTPDISNVKAPFINRYYGKAHEVL
Sbjct: 301 EKMKFYKFYPVQTSDTPDISNVKAPFINRYYGKAHEVL 338

BLAST of MC10g0603 vs. ExPASy TrEMBL
Match: A0A6J1C502 (uncharacterized protein LOC111008415 OS=Momordica charantia OX=3673 GN=LOC111008415 PE=4 SV=1)

HSP 1 Score: 666 bits (1718), Expect = 1.73e-241
Identity = 338/338 (100.00%), Postives = 338/338 (100.00%), Query Frame = 0

Query: 1   MRKGTKRKTARKEEDKPVEPKRKEAPSRAKRAKLPKPESEPEYFEDKRNLEDLWKAAFPV 60
           MRKGTKRKTARKEEDKPVEPKRKEAPSRAKRAKLPKPESEPEYFEDKRNLEDLWKAAFPV
Sbjct: 1   MRKGTKRKTARKEEDKPVEPKRKEAPSRAKRAKLPKPESEPEYFEDKRNLEDLWKAAFPV 60

Query: 61  GTEWDQLDTVYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENKVICIPAV 120
           GTEWDQLDTVYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENKVICIPAV
Sbjct: 61  GTEWDQLDTVYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENKVICIPAV 120

Query: 121 VAVVSPFPPSDKIGINSVQREAEEIVPMKQMKMDWVPYIPLEKRESRVDKLKSQIFILSC 180
           VAVVSPFPPSDKIGINSVQREAEEIVPMKQMKMDWVPYIPLEKRESRVDKLKSQIFILSC
Sbjct: 121 VAVVSPFPPSDKIGINSVQREAEEIVPMKQMKMDWVPYIPLEKRESRVDKLKSQIFILSC 180

Query: 181 TQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDEFEQSTEVPIIFPIDPKPVFCEFDWELD 240
           TQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDEFEQSTEVPIIFPIDPKPVFCEFDWELD
Sbjct: 181 TQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDEFEQSTEVPIIFPIDPKPVFCEFDWELD 240

Query: 241 ELEEFTDKLIEEEELSESQKDAFKDFVKEKVREAKKANREAREARKKAIEEMSKETKEAF 300
           ELEEFTDKLIEEEELSESQKDAFKDFVKEKVREAKKANREAREARKKAIEEMSKETKEAF
Sbjct: 241 ELEEFTDKLIEEEELSESQKDAFKDFVKEKVREAKKANREAREARKKAIEEMSKETKEAF 300

Query: 301 EKMKFYKFYPVQTPDTPDISNVKAPFINRYYGKAHEVL 338
           EKMKFYKFYPVQTPDTPDISNVKAPFINRYYGKAHEVL
Sbjct: 301 EKMKFYKFYPVQTPDTPDISNVKAPFINRYYGKAHEVL 338

BLAST of MC10g0603 vs. ExPASy TrEMBL
Match: A0A0A0KUL7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G037620 PE=4 SV=1)

HSP 1 Score: 637 bits (1642), Expect = 6.63e-230
Identity = 318/338 (94.08%), Postives = 328/338 (97.04%), Query Frame = 0

Query: 1   MRKGTKRKTARKEEDKPVEPKRKEAPSRAKRAKLPKPESEPEYFEDKRNLEDLWKAAFPV 60
           MRKGTKRK ARKEEDKP EPK  +APSRAKR KLPKPESEPEYFEDKRN+EDLWKAAFPV
Sbjct: 1   MRKGTKRKAARKEEDKPAEPKPDKAPSRAKRTKLPKPESEPEYFEDKRNMEDLWKAAFPV 60

Query: 61  GTEWDQLDTVYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENKVICIPAV 120
           GTEWDQLD+VYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENKVICIP V
Sbjct: 61  GTEWDQLDSVYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENKVICIPVV 120

Query: 121 VAVVSPFPPSDKIGINSVQREAEEIVPMKQMKMDWVPYIPLEKRESRVDKLKSQIFILSC 180
           VAV SPFPPSDKIGINSVQREAEEI+PMKQMKMDWVPYIPLEKR+ RVDKLKSQIFILSC
Sbjct: 121 VAVASPFPPSDKIGINSVQREAEEIIPMKQMKMDWVPYIPLEKRDRRVDKLKSQIFILSC 180

Query: 181 TQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDEFEQSTEVPIIFPIDPKPVFCEFDWELD 240
           TQRRAALKHLKIDR+KKYEYCLPYFYQPFK+DEFEQSTEVPIIFP+DPKPVFCEFDWE D
Sbjct: 181 TQRRAALKHLKIDRLKKYEYCLPYFYQPFKDDEFEQSTEVPIIFPVDPKPVFCEFDWEFD 240

Query: 241 ELEEFTDKLIEEEELSESQKDAFKDFVKEKVREAKKANREAREARKKAIEEMSKETKEAF 300
           ELEEFTDKLIEEEELSESQKDAFKDFV+EKVREAKKANREAREARKKAIEEMS ETKEAF
Sbjct: 241 ELEEFTDKLIEEEELSESQKDAFKDFVREKVREAKKANREAREARKKAIEEMSNETKEAF 300

Query: 301 EKMKFYKFYPVQTPDTPDISNVKAPFINRYYGKAHEVL 338
           EKMKFYKFYPVQTPD+PDISNVKAPFINRYYGKAHEVL
Sbjct: 301 EKMKFYKFYPVQTPDSPDISNVKAPFINRYYGKAHEVL 338

BLAST of MC10g0603 vs. ExPASy TrEMBL
Match: A0A1S3BXT3 (uncharacterized protein LOC103494391 OS=Cucumis melo OX=3656 GN=LOC103494391 PE=4 SV=1)

HSP 1 Score: 625 bits (1613), Expect = 1.74e-225
Identity = 313/338 (92.60%), Postives = 324/338 (95.86%), Query Frame = 0

Query: 1   MRKGTKRKTARKEEDKPVEPKRKEAPSRAKRAKLPKPESEPEYFEDKRNLEDLWKAAFPV 60
           MRKGTKRK  RKEEDKP EPK +++P RAKR KLPKPESEPEYFEDKRN+EDLWKAAFPV
Sbjct: 1   MRKGTKRKATRKEEDKPAEPKPEKSPPRAKRTKLPKPESEPEYFEDKRNMEDLWKAAFPV 60

Query: 61  GTEWDQLDTVYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENKVICIPAV 120
           GTEWDQLD+VY+FNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENK+  IP V
Sbjct: 61  GTEWDQLDSVYKFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENKLTIIPVV 120

Query: 121 VAVVSPFPPSDKIGINSVQREAEEIVPMKQMKMDWVPYIPLEKRESRVDKLKSQIFILSC 180
           VAV SPFPPSDKIGINSVQREAEEIVPMKQMKMDWVPYIPLEKR+ RVDKLKSQIFILSC
Sbjct: 121 VAVASPFPPSDKIGINSVQREAEEIVPMKQMKMDWVPYIPLEKRDRRVDKLKSQIFILSC 180

Query: 181 TQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDEFEQSTEVPIIFPIDPKPVFCEFDWELD 240
           TQRRAALKHLKIDRVKKYEYCLPYFYQPFK+DEFEQSTEVPIIFP+DPKPVFCEFDWELD
Sbjct: 181 TQRRAALKHLKIDRVKKYEYCLPYFYQPFKDDEFEQSTEVPIIFPVDPKPVFCEFDWELD 240

Query: 241 ELEEFTDKLIEEEELSESQKDAFKDFVKEKVREAKKANREAREARKKAIEEMSKETKEAF 300
           ELEEFTDKLIE EELSESQKDAFKDFV+EKVRE KKANREAREARKKAIEEMS ETKEAF
Sbjct: 241 ELEEFTDKLIENEELSESQKDAFKDFVREKVRETKKANREAREARKKAIEEMSNETKEAF 300

Query: 301 EKMKFYKFYPVQTPDTPDISNVKAPFINRYYGKAHEVL 338
           EKMKFYKFYPVQTPDTPDISNVKAPFINRYYGKAHEVL
Sbjct: 301 EKMKFYKFYPVQTPDTPDISNVKAPFINRYYGKAHEVL 338

BLAST of MC10g0603 vs. ExPASy TrEMBL
Match: A0A6J1IV15 (protein HEAT INTOLERANT 4-like OS=Cucurbita maxima OX=3661 GN=LOC111480844 PE=4 SV=1)

HSP 1 Score: 623 bits (1606), Expect = 2.03e-224
Identity = 315/338 (93.20%), Postives = 324/338 (95.86%), Query Frame = 0

Query: 1   MRKGTKRKTARKEEDKPVEPKRKEAPSRAKRAKLPKPESEPEYFEDKRNLEDLWKAAFPV 60
           MRKGTKRK  RKE+ KP +PK KE PSR KR K+PKPESEPEYFED+RNLEDLWKAAFPV
Sbjct: 1   MRKGTKRKATRKEDHKPAQPKPKEVPSRTKRIKVPKPESEPEYFEDQRNLEDLWKAAFPV 60

Query: 61  GTEWDQLDTVYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENKVICIPAV 120
           GTEWDQLD+VYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVP+KGENKV  IPAV
Sbjct: 61  GTEWDQLDSVYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPYKGENKVTVIPAV 120

Query: 121 VAVVSPFPPSDKIGINSVQREAEEIVPMKQMKMDWVPYIPLEKRESRVDKLKSQIFILSC 180
           VAVVSPFPPSDKIGINSVQREAEEIVPMKQMKMDWVPYIPLEKR+S+VDKLKSQIFILSC
Sbjct: 121 VAVVSPFPPSDKIGINSVQREAEEIVPMKQMKMDWVPYIPLEKRDSQVDKLKSQIFILSC 180

Query: 181 TQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDEFEQSTEVPIIFPIDPKPVFCEFDWELD 240
           TQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDE EQSTEVPIIFPIDPKPVFCEFDWELD
Sbjct: 181 TQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDEIEQSTEVPIIFPIDPKPVFCEFDWELD 240

Query: 241 ELEEFTDKLIEEEELSESQKDAFKDFVKEKVREAKKANREAREARKKAIEEMSKETKEAF 300
           ELEEFTDKLIEEEEL E+QKDAFK FVKEKVREAKKANREAREARKKAIEEMSKETKEAF
Sbjct: 241 ELEEFTDKLIEEEELPENQKDAFKAFVKEKVREAKKANREAREARKKAIEEMSKETKEAF 300

Query: 301 EKMKFYKFYPVQTPDTPDISNVKAPFINRYYGKAHEVL 338
           EKMKFYKFYPVQT DTPDISNVKAPFINRYYGKAHEVL
Sbjct: 301 EKMKFYKFYPVQTSDTPDISNVKAPFINRYYGKAHEVL 338

BLAST of MC10g0603 vs. ExPASy TrEMBL
Match: A0A6J1EUP5 (protein HEAT INTOLERANT 4-like OS=Cucurbita moschata OX=3662 GN=LOC111436085 PE=4 SV=1)

HSP 1 Score: 622 bits (1604), Expect = 3.95e-224
Identity = 316/338 (93.49%), Postives = 324/338 (95.86%), Query Frame = 0

Query: 1   MRKGTKRKTARKEEDKPVEPKRKEAPSRAKRAKLPKPESEPEYFEDKRNLEDLWKAAFPV 60
           MRKGTKRKT +KEEDKP EPK  E PSR+KR K+PKPESEP+YFEDKRNLEDLWKAAFPV
Sbjct: 1   MRKGTKRKTTQKEEDKPAEPKPVE-PSRSKRTKVPKPESEPQYFEDKRNLEDLWKAAFPV 60

Query: 61  GTEWDQLDTVYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENKVICIPAV 120
           GTEWDQLDTVYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENKVICIP V
Sbjct: 61  GTEWDQLDTVYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENKVICIPVV 120

Query: 121 VAVVSPFPPSDKIGINSVQREAEEIVPMKQMKMDWVPYIPLEKRESRVDKLKSQIFILSC 180
           VAV SPFPPSDKIGINSVQREAEEIVPMKQMKMDWVPYIPL++R S+VDKLKSQIFILSC
Sbjct: 121 VAVASPFPPSDKIGINSVQREAEEIVPMKQMKMDWVPYIPLDQRGSQVDKLKSQIFILSC 180

Query: 181 TQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDEFEQSTEVPIIFPIDPKPVFCEFDWELD 240
           TQRRA LKHLKIDRVKK+EYCLPYFY PFKEDE EQSTEVPIIFPIDPKPVFCEFDWELD
Sbjct: 181 TQRRAGLKHLKIDRVKKFEYCLPYFYHPFKEDEVEQSTEVPIIFPIDPKPVFCEFDWELD 240

Query: 241 ELEEFTDKLIEEEELSESQKDAFKDFVKEKVREAKKANREAREARKKAIEEMSKETKEAF 300
           ELEEFTDKLIEEEEL ESQKDAFKDFVKEKVREAKKANR AREARKKAIEEMSKETKEAF
Sbjct: 241 ELEEFTDKLIEEEELPESQKDAFKDFVKEKVREAKKANRLAREARKKAIEEMSKETKEAF 300

Query: 301 EKMKFYKFYPVQTPDTPDISNVKAPFINRYYGKAHEVL 338
           EKMKFYKFYPVQTPDTPDISNVKAPFINRYYGKAHEVL
Sbjct: 301 EKMKFYKFYPVQTPDTPDISNVKAPFINRYYGKAHEVL 337

BLAST of MC10g0603 vs. TAIR 10
Match: AT5G10010.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: nucleolus; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G64910.1); Has 33260 Blast hits to 16857 proteins in 1270 species: Archae - 88; Bacteria - 3040; Metazoa - 11915; Fungi - 3137; Plants - 1371; Viruses - 424; Other Eukaryotes - 13285 (source: NCBI BLink). )

HSP 1 Score: 482.3 bits (1240), Expect = 3.3e-136
Identity = 236/337 (70.03%), Postives = 289/337 (85.76%), Query Frame = 0

Query: 2   RKGTKRKTARKEEDKPVEPKRKEAPSRAKRAKLPKPESEPEYFEDKRNLEDLWKAAFPVG 61
           R+G KRK A K +D  ++ ++K  P +AK+ +  K + EP YFE+KR+LEDLWK AFPVG
Sbjct: 101 RRGGKRKRATK-KDTEIKDEKKPVP-KAKKPRAAKVKEEPVYFEEKRSLEDLWKVAFPVG 160

Query: 62  TEWDQLDTVYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENKVICIPAVV 121
           TEWDQLD +Y+FNW+F NLE+A EEGGKLYG+KVY+FGCTEPQLVP+KG NK++ +PAVV
Sbjct: 161 TEWDQLDALYEFNWDFQNLEEALEEGGKLYGKKVYVFGCTEPQLVPYKGANKIVHVPAVV 220

Query: 122 AVVSPFPPSDKIGINSVQREAEEIVPMKQMKMDWVPYIPLEKRESRVDKLKSQIFILSCT 181
            + SPFPPSDKIGI SVQRE EEI+PMK+MKMDW+PYIP+EKR+ +VDK+ SQIF L CT
Sbjct: 221 VIESPFPPSDKIGITSVQREVEEIIPMKKMKMDWLPYIPIEKRDRQVDKMNSQIFTLGCT 280

Query: 182 QRRAALKHLKIDRVKKYEYCLPYFYQPFKEDEFEQSTEVPIIFPIDPKPVFCEFDWELDE 241
           QRR+AL+H+K D++KK+EYCLPYFYQPFKEDE EQSTEV I+FP +P PV CEFDWE DE
Sbjct: 281 QRRSALRHMKEDQLKKFEYCLPYFYQPFKEDELEQSTEVQIMFPSEP-PVVCEFDWEFDE 340

Query: 242 LEEFTDKLIEEEELSESQKDAFKDFVKEKVREAKKANREAREARKKAIEEMSKETKEAFE 301
           L+EF DKL+EEE L   Q D FK++VKE+VR AKKANREA++ARKKAIEEMS++TK+AF+
Sbjct: 341 LQEFVDKLVEEEALPAEQADEFKEYVKEQVRAAKKANREAKDARKKAIEEMSEDTKQAFQ 400

Query: 302 KMKFYKFYPVQTPDTPDISNVKAPFINRYYGKAHEVL 339
           KMKFYKFYP  +PDTPD+S V++PFINRYYGKAHEVL
Sbjct: 401 KMKFYKFYPQPSPDTPDVSGVQSPFINRYYGKAHEVL 434

BLAST of MC10g0603 vs. TAIR 10
Match: AT5G64910.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G10010.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 340.1 bits (871), Expect = 2.0e-93
Identity = 180/324 (55.56%), Postives = 234/324 (72.22%), Query Frame = 0

Query: 1   MRKGTKRKTARKEEDKPVEPKRKEAPSRAKRAKLPKPE-SEPEYFEDKRNLEDLWKAAFP 60
           +R+G +++  + E +K V       P   KRAK  K + SEPEYFE+KRNLEDLWKA F 
Sbjct: 141 LRRGKRKRGTKTEAEKKV-----STPRAKKRAKTTKAQASEPEYFEEKRNLEDLWKATFS 200

Query: 61  VGTEWDQLDTVYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENKVICIPA 120
           VGTEWDQ D + +FNW+F+NLE+A EEGG+LYG++VY+FGCTE   V +K ENK + +P 
Sbjct: 201 VGTEWDQQDALNEFNWDFTNLEEALEEGGELYGKQVYVFGCTESHSVTYKDENKDVLVPV 260

Query: 121 VVAVVSPFPPSDKIGINSVQREAEEIVPMKQMKMDWVPYIPLEKRESRVDKLKSQIFILS 180
           VV + SP PPSD+IG+ SVQ E  EI+ MK MKM WVPYIPLE+R+ +VD     IFIL 
Sbjct: 261 VVCIDSPIPPSDEIGVASVQGEVGEIIAMKTMKMAWVPYIPLEQRDRQVDNKNFPIFILG 320

Query: 181 CTQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDEFEQSTEVPIIFPIDPKPVFCEFDWEL 240
           CTQRR+ALKHL  DRVKK+ YCLPY   P+K D+ E+ST V I+FP +P PV CE+DW  
Sbjct: 321 CTQRRSALKHLPDDRVKKFNYCLPYINNPYKVDDSEKSTVVKIMFPSEP-PVECEYDWVK 380

Query: 241 DELEEFTDKLIEEEELSESQKDAFKDFVKEKVREAKKANREAREARKKAIEEMSKETKEA 300
             +EEFTD LI EE L   QK AF++FVKEK  +A  A   A+EA +KA E +S+ETK+A
Sbjct: 381 SVIEEFTDSLINEEVLLPEQKVAFEEFVKEKSDKAMAAYDTAQEALEKAKEGLSEETKKA 440

Query: 301 FEKMKFYKFYPVQTPDTPDISNVK 324
           +++M+ YKFYP+ +PDTP  + ++
Sbjct: 441 YQEMRLYKFYPLPSPDTPHTAGIE 458

BLAST of MC10g0603 vs. TAIR 10
Match: AT5G64910.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G10010.1). )

HSP 1 Score: 333.6 bits (854), Expect = 1.9e-91
Identity = 179/324 (55.25%), Postives = 233/324 (71.91%), Query Frame = 0

Query: 1   MRKGTKRKTARKEEDKPVEPKRKEAPSRAKRAKLPKPE-SEPEYFEDKRNLEDLWKAAFP 60
           +R+G +++  + E +K V       P   KRAK  K + SEPEYFE+KRNLEDLWKA F 
Sbjct: 141 LRRGKRKRGTKTEAEKKV-----STPRAKKRAKTTKAQASEPEYFEEKRNLEDLWKATFS 200

Query: 61  VGTEWDQLDTVYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENKVICIPA 120
           VGTEWDQ D + +FNW+F+NLE+A EEGG+LYG++VY+FGCTE     +K ENK + +P 
Sbjct: 201 VGTEWDQQDALNEFNWDFTNLEEALEEGGELYGKQVYVFGCTE---FTYKDENKDVLVPV 260

Query: 121 VVAVVSPFPPSDKIGINSVQREAEEIVPMKQMKMDWVPYIPLEKRESRVDKLKSQIFILS 180
           VV + SP PPSD+IG+ SVQ E  EI+ MK MKM WVPYIPLE+R+ +VD     IFIL 
Sbjct: 261 VVCIDSPIPPSDEIGVASVQGEVGEIIAMKTMKMAWVPYIPLEQRDRQVDNKNFPIFILG 320

Query: 181 CTQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDEFEQSTEVPIIFPIDPKPVFCEFDWEL 240
           CTQRR+ALKHL  DRVKK+ YCLPY   P+K D+ E+ST V I+FP +P PV CE+DW  
Sbjct: 321 CTQRRSALKHLPDDRVKKFNYCLPYINNPYKVDDSEKSTVVKIMFPSEP-PVECEYDWVK 380

Query: 241 DELEEFTDKLIEEEELSESQKDAFKDFVKEKVREAKKANREAREARKKAIEEMSKETKEA 300
             +EEFTD LI EE L   QK AF++FVKEK  +A  A   A+EA +KA E +S+ETK+A
Sbjct: 381 SVIEEFTDSLINEEVLLPEQKVAFEEFVKEKSDKAMAAYDTAQEALEKAKEGLSEETKKA 440

Query: 301 FEKMKFYKFYPVQTPDTPDISNVK 324
           +++M+ YKFYP+ +PDTP  + ++
Sbjct: 441 YQEMRLYKFYPLPSPDTPHTAGIE 455

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A2RVJ84.6e-13570.03Protein HEAT INTOLERANT 4 OS=Arabidopsis thaliana OX=3702 GN=HIT4 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022136810.13.57e-241100.00uncharacterized protein LOC111008415 [Momordica charantia][more]
XP_038878042.13.36e-23094.97protein HEAT INTOLERANT 4-like [Benincasa hispida][more]
XP_004149148.11.37e-22994.08protein HEAT INTOLERANT 4-like [Cucumis sativus] >XP_031740732.1 protein HEAT IN... [more]
XP_008453747.13.60e-22592.60PREDICTED: uncharacterized protein LOC103494391 [Cucumis melo][more]
XP_022981812.14.20e-22493.20protein HEAT INTOLERANT 4-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1C5021.73e-241100.00uncharacterized protein LOC111008415 OS=Momordica charantia OX=3673 GN=LOC111008... [more]
A0A0A0KUL76.63e-23094.08Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G037620 PE=4 SV=1[more]
A0A1S3BXT31.74e-22592.60uncharacterized protein LOC103494391 OS=Cucumis melo OX=3656 GN=LOC103494391 PE=... [more]
A0A6J1IV152.03e-22493.20protein HEAT INTOLERANT 4-like OS=Cucurbita maxima OX=3661 GN=LOC111480844 PE=4 ... [more]
A0A6J1EUP53.95e-22493.49protein HEAT INTOLERANT 4-like OS=Cucurbita moschata OX=3662 GN=LOC111436085 PE=... [more]
Match NameE-valueIdentityDescription
AT5G10010.13.3e-13670.03unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT5G64910.12.0e-9355.56unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT5G64910.21.9e-9155.25unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 264..298
NoneNo IPR availableGENE3D6.10.250.2770coord: 1..76
e-value: 1.4E-14
score: 56.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 8..43
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..43
NoneNo IPR availablePANTHERPTHR33704:SF1PROTEIN HEAT INTOLERANT 4-RELATEDcoord: 1..337
IPR039313Protein HEAT INTOLERANT 4PANTHERPTHR33704PROTEIN HEAT INTOLERANT 4-RELATEDcoord: 1..337

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC10g0603.1MC10g0603.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1900034 regulation of cellular response to heat