MC10g0355 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC10g0355
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Trypsin inhibitor 1-like
Location	MC10: 2871608 .. 2871886 (-)
RNA-Seq Expression	MC10g0355
Synteny	MC10g0355

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAGCAAGAAGATTGTTGTTGTGGTGGCGCTGGTGGCGATGATGCTGGTCGCAACATCGGCCGCCTTCGACGAGGGCGACACGAGACCCACGAGACCTCTCGTCTCCGACGATGGAGCTGTAGTTGGTCAAGGCATGAATGATTATCCAAGAAAAATGTTTGTGAAAGTTGTGTATTACGAGAATCAAAGAGGGTGTCCGAGAATCTTGAAGCAATGCAAACAGGACTCGGATTGCCCCGGTGAGTGTATTTGCATGGCGCATGGGTTCTGCGGT

mRNA sequence

ATGGAGAGCAAGAAGATTGTTGTTGTGGTGGCGCTGGTGGCGATGATGCTGGTCGCAACATCGGCCGCCTTCGACGAGGGCGACACGAGACCCACGAGACCTCTCGTCTCCGACGATGGAGCTGTAGTTGGTCAAGGCATGAATGATTATCCAAGAAAAATGTTTGTGAAAGTTGTGTATTACGAGAATCAAAGAGGGTGTCCGAGAATCTTGAAGCAATGCAAACAGGACTCGGATTGCCCCGGTGAGTGTATTTGCATGGCGCATGGGTTCTGCGGT

Coding sequence (CDS)

ATGGAGAGCAAGAAGATTGTTGTTGTGGTGGCGCTGGTGGCGATGATGCTGGTCGCAACATCGGCCGCCTTCGACGAGGGCGACACGAGACCCACGAGACCTCTCGTCTCCGACGATGGAGCTGTAGTTGGTCAAGGCATGAATGATTATCCAAGAAAAATGTTTGTGAAAGTTGTGTATTACGAGAATCAAAGAGGGTGTCCGAGAATCTTGAAGCAATGCAAACAGGACTCGGATTGCCCCGGTGAGTGTATTTGCATGGCGCATGGGTTCTGCGGT

Protein sequence

MESKKIVVVVALVAMMLVATSAAFDEGDTRPTRPLVSDDGAVVGQGMNDYPRKMFVKVVYYENQRGCPRILKQCKQDSDCPGECICMAHGFCG

Homology

[Close]

BLAST of MC10g0355 vs. ExPASy Swiss-Prot
Match: Q9S747 (Trypsin inhibitor 3 OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 1.0e-12
Identity = 29/30 (96.67%), Postives = 30/30 (100.00%), Query Frame = 0


Query: 64 QRGCPRILKQCKQDSDCPGECICMAHGFCG 94
          +RGCPRILKQCKQDSDCPGECICMAHGFCG
Sbjct: 1  ERGCPRILKQCKQDSDCPGECICMAHGFCG 30

[Close]

BLAST of MC10g0355 vs. ExPASy Swiss-Prot
Match: P10294 (Trypsin inhibitor 1 OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 2.6e-11
Identity = 27/30 (90.00%), Postives = 29/30 (96.67%), Query Frame = 0


Query: 64 QRGCPRILKQCKQDSDCPGECICMAHGFCG 94
          +R CPRILKQCK+DSDCPGECICMAHGFCG
Sbjct: 1  ERRCPRILKQCKRDSDCPGECICMAHGFCG 30

[Close]

BLAST of MC10g0355 vs. ExPASy Swiss-Prot
Match: P82410 (Trypsin inhibitor 3 OS=Momordica cochinchinensis OX=3674 PE=1 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 1.2e-08
Identity = 22/30 (73.33%), Postives = 27/30 (90.00%), Query Frame = 0


Query: 64 QRGCPRILKQCKQDSDCPGECICMAHGFCG 94
          QR CPRILK+C++DSDCPGECIC  +G+CG
Sbjct: 1  QRACPRILKKCRRDSDCPGECICKENGYCG 30

[Close]

BLAST of MC10g0355 vs. ExPASy Swiss-Prot
Match: P07853 (Trypsin inhibitor 4 OS=Cucurbita maxima OX=3661 PE=1 SV=2)

HSP 1 Score: 54.3 bits (129), Expect = 8.6e-07
Identity = 19/30 (63.33%), Postives = 25/30 (83.33%), Query Frame = 0


Query: 64 QRGCPRILKQCKQDSDCPGECICMAHGFCG 94
          +R CPRIL +CK+DSDC  EC+C+ HG+CG
Sbjct: 3  ERVCPRILMKCKKDSDCLAECVCLEHGYCG 32

[Close]

BLAST of MC10g0355 vs. ExPASy Swiss-Prot
Match: P01074 (Trypsin inhibitor 1 OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 1.1e-06
Identity = 19/29 (65.52%), Postives = 24/29 (82.76%), Query Frame = 0


Query: 65 RGCPRILKQCKQDSDCPGECICMAHGFCG 94
          R CPRIL +CK+DSDC  EC+C+ HG+CG
Sbjct: 1  RVCPRILMECKKDSDCLAECVCLEHGYCG 29

[Close]

BLAST of MC10g0355 vs. NCBI nr
Match: AIZ03437.1 (TIPRE1 precursor, partial [Momordica anigosantha])

HSP 1 Score: 82.0 bits (201), Expect = 7.14e-18
Identity = 44/83 (53.01%), Postives = 55/83 (66.27%), Query Frame = 0


Query: 11 ALVAMMLVATSAAFDEGDTRPTRPLVSDDGAVVGQGMNDYPRKMFVKVVYYENQRGCPRI 70
          ALVAMMLVATSA F+ GDT     L+S+  A  GQ +N          VY E QR CP+I
Sbjct: 3  ALVAMMLVATSADFNGGDTIH---LISNGRAQTGQDINSGG-------VYSEEQRACPKI 62

Query: 71 LKQCKQDSDCPGECICMAHGFCG 93
          LK+C++DSDCPG C+C  +G+CG
Sbjct: 63 LKRCRRDSDCPGACVCQDNGYCG 75

[Close]

BLAST of MC10g0355 vs. NCBI nr
Match: AIZ03439.1 (TIPRE3 precursor, partial [Momordica anigosantha])

HSP 1 Score: 84.3 bits (207), Expect = 1.40e-17
Identity = 44/82 (53.66%), Postives = 55/82 (67.07%), Query Frame = 0


Query: 12 LVAMMLVATSAAFDEGDTRPTRPLVSDDGAVVGQGMNDYPRKMFVKVVYYENQRGCPRIL 71
          LVA MLVATSA F++GDT     L+S+D A  GQ +N          VY E QR CPRIL
Sbjct: 4  LVATMLVATSADFNDGDTID---LISNDRAQTGQDINGGG-------VYSEEQRACPRIL 63

Query: 72 KQCKQDSDCPGECICMAHGFCG 93
          K+C++DSDCPG C+C  +G+CG
Sbjct: 64 KRCRRDSDCPGACVCQGNGYCG 75

[Close]

BLAST of MC10g0355 vs. NCBI nr
Match: AIZ03438.1 (TIPRE2 precursor, partial [Momordica anigosantha])

HSP 1 Score: 82.0 bits (201), Expect = 3.17e-17
Identity = 44/83 (53.01%), Postives = 55/83 (66.27%), Query Frame = 0


Query: 11 ALVAMMLVATSAAFDEGDTRPTRPLVSDDGAVVGQGMNDYPRKMFVKVVYYENQRGCPRI 70
          ALVAMMLVATSA F+ GDT     L+S+  A  GQ +N          VY E QR CP+I
Sbjct: 3  ALVAMMLVATSADFNGGDTIH---LISNGRAQTGQDINSGG-------VYSEEQRACPKI 62

Query: 71 LKQCKQDSDCPGECICMAHGFCG 93
          LK+C++DSDCPG C+C  +G+CG
Sbjct: 63 LKRCRRDSDCPGACVCQDNGYCG 75

[Close]

BLAST of MC10g0355 vs. NCBI nr
Match: AIZ03440.1 (TIPRE4 precursor, partial [Momordica anigosantha])

HSP 1 Score: 84.3 bits (207), Expect = 3.67e-17
Identity = 44/82 (53.66%), Postives = 55/82 (67.07%), Query Frame = 0


Query: 12 LVAMMLVATSAAFDEGDTRPTRPLVSDDGAVVGQGMNDYPRKMFVKVVYYENQRGCPRIL 71
          LVA MLVATSA F++GDT     L+S+D A  GQ +N          VY E QR CPRIL
Sbjct: 4  LVATMLVATSADFNDGDTID---LISNDRAQTGQDINGGG-------VYSEEQRACPRIL 63

Query: 72 KQCKQDSDCPGECICMAHGFCG 93
          K+C++DSDCPG C+C  +G+CG
Sbjct: 64 KRCRRDSDCPGACVCQGNGYCG 75

[Close]

BLAST of MC10g0355 vs. NCBI nr
Match: AIZ03441.1 (TIPRE5 precursor, partial [Momordica friesiorum])

HSP 1 Score: 79.0 bits (193), Expect = 1.17e-16
Identity = 43/83 (51.81%), Postives = 54/83 (65.06%), Query Frame = 0


Query: 11 ALVAMMLVATSAAFDEGDTRPTRPLVSDDGAVVGQGMNDYPRKMFVKVVYYENQRGCPRI 70
          ALVAMMLVATSA  + GDT     L+S+  A  GQ +N          VY E QR CP+I
Sbjct: 3  ALVAMMLVATSADSNGGDTIH---LISNGRAQTGQDINSGG-------VYSEEQRACPKI 62

Query: 71 LKQCKQDSDCPGECICMAHGFCG 93
          LK+C++DSDCPG C+C  +G+CG
Sbjct: 63 LKRCRRDSDCPGACVCQDNGYCG 75

[Close]

BLAST of MC10g0355 vs. ExPASy TrEMBL
Match: A0A0A7HG47 (TIPRE1 (Fragment) OS=Momordica anigosantha OX=703354 GN=TIPRE1 PE=3 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 3.45e-18
Identity = 44/83 (53.01%), Postives = 55/83 (66.27%), Query Frame = 0


Query: 11 ALVAMMLVATSAAFDEGDTRPTRPLVSDDGAVVGQGMNDYPRKMFVKVVYYENQRGCPRI 70
          ALVAMMLVATSA F+ GDT     L+S+  A  GQ +N          VY E QR CP+I
Sbjct: 3  ALVAMMLVATSADFNGGDTIH---LISNGRAQTGQDINSGG-------VYSEEQRACPKI 62

Query: 71 LKQCKQDSDCPGECICMAHGFCG 93
          LK+C++DSDCPG C+C  +G+CG
Sbjct: 63 LKRCRRDSDCPGACVCQDNGYCG 75

[Close]

BLAST of MC10g0355 vs. ExPASy TrEMBL
Match: A0A0A7HF94 (TIPRE3 (Fragment) OS=Momordica anigosantha OX=703354 GN=TIPRE3 PE=3 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 6.78e-18
Identity = 44/82 (53.66%), Postives = 55/82 (67.07%), Query Frame = 0


Query: 12 LVAMMLVATSAAFDEGDTRPTRPLVSDDGAVVGQGMNDYPRKMFVKVVYYENQRGCPRIL 71
          LVA MLVATSA F++GDT     L+S+D A  GQ +N          VY E QR CPRIL
Sbjct: 4  LVATMLVATSADFNDGDTID---LISNDRAQTGQDINGGG-------VYSEEQRACPRIL 63

Query: 72 KQCKQDSDCPGECICMAHGFCG 93
          K+C++DSDCPG C+C  +G+CG
Sbjct: 64 KRCRRDSDCPGACVCQGNGYCG 75

[Close]

BLAST of MC10g0355 vs. ExPASy TrEMBL
Match: A0A0A7HF33 (TIPRE2 (Fragment) OS=Momordica anigosantha OX=703354 GN=TIPRE2 PE=3 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.53e-17
Identity = 44/83 (53.01%), Postives = 55/83 (66.27%), Query Frame = 0


Query: 11 ALVAMMLVATSAAFDEGDTRPTRPLVSDDGAVVGQGMNDYPRKMFVKVVYYENQRGCPRI 70
          ALVAMMLVATSA F+ GDT     L+S+  A  GQ +N          VY E QR CP+I
Sbjct: 3  ALVAMMLVATSADFNGGDTIH---LISNGRAQTGQDINSGG-------VYSEEQRACPKI 62

Query: 71 LKQCKQDSDCPGECICMAHGFCG 93
          LK+C++DSDCPG C+C  +G+CG
Sbjct: 63 LKRCRRDSDCPGACVCQDNGYCG 75

[Close]

BLAST of MC10g0355 vs. ExPASy TrEMBL
Match: A0A0A7HIT2 (TIPRE4 (Fragment) OS=Momordica anigosantha OX=703354 GN=TIPRE4 PE=3 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.78e-17
Identity = 44/82 (53.66%), Postives = 55/82 (67.07%), Query Frame = 0


Query: 12 LVAMMLVATSAAFDEGDTRPTRPLVSDDGAVVGQGMNDYPRKMFVKVVYYENQRGCPRIL 71
          LVA MLVATSA F++GDT     L+S+D A  GQ +N          VY E QR CPRIL
Sbjct: 4  LVATMLVATSADFNDGDTID---LISNDRAQTGQDINGGG-------VYSEEQRACPRIL 63

Query: 72 KQCKQDSDCPGECICMAHGFCG 93
          K+C++DSDCPG C+C  +G+CG
Sbjct: 64 KRCRRDSDCPGACVCQGNGYCG 75

[Close]

BLAST of MC10g0355 vs. ExPASy TrEMBL
Match: A0A0A7HIA9 (TIPRE5 (Fragment) OS=Momordica friesiorum OX=703365 GN=TIPRE5 PE=3 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 5.64e-17
Identity = 43/83 (51.81%), Postives = 54/83 (65.06%), Query Frame = 0


Query: 11 ALVAMMLVATSAAFDEGDTRPTRPLVSDDGAVVGQGMNDYPRKMFVKVVYYENQRGCPRI 70
          ALVAMMLVATSA  + GDT     L+S+  A  GQ +N          VY E QR CP+I
Sbjct: 3  ALVAMMLVATSADSNGGDTIH---LISNGRAQTGQDINSGG-------VYSEEQRACPKI 62

Query: 71 LKQCKQDSDCPGECICMAHGFCG 93
          LK+C++DSDCPG C+C  +G+CG
Sbjct: 63 LKRCRRDSDCPGACVCQDNGYCG 75

The following BLAST results are available for this feature:

BLAST of MC10g0355 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9S747	1.0e-12	96.67	Trypsin inhibitor 3 OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P10294	2.6e-11	90.00	Trypsin inhibitor 1 OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P82410	1.2e-08	73.33	Trypsin inhibitor 3 OS=Momordica cochinchinensis OX=3674 PE=1 SV=1	[more]
P07853	8.6e-07	63.33	Trypsin inhibitor 4 OS=Cucurbita maxima OX=3661 PE=1 SV=2	[more]
P01074	1.1e-06	65.52	Trypsin inhibitor 1 OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]

BLAST of MC10g0355 vs. NCBI nr


Match Name	E-value	Identity	Description
AIZ03437.1	7.14e-18	53.01	TIPRE1 precursor, partial [Momordica anigosantha]	[more]
AIZ03439.1	1.40e-17	53.66	TIPRE3 precursor, partial [Momordica anigosantha]	[more]
AIZ03438.1	3.17e-17	53.01	TIPRE2 precursor, partial [Momordica anigosantha]	[more]
AIZ03440.1	3.67e-17	53.66	TIPRE4 precursor, partial [Momordica anigosantha]	[more]
AIZ03441.1	1.17e-16	51.81	TIPRE5 precursor, partial [Momordica friesiorum]	[more]

BLAST of MC10g0355 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A7HG47	3.45e-18	53.01	TIPRE1 (Fragment) OS=Momordica anigosantha OX=703354 GN=TIPRE1 PE=3 SV=1	[more]
A0A0A7HF94	6.78e-18	53.66	TIPRE3 (Fragment) OS=Momordica anigosantha OX=703354 GN=TIPRE3 PE=3 SV=1	[more]
A0A0A7HF33	1.53e-17	53.01	TIPRE2 (Fragment) OS=Momordica anigosantha OX=703354 GN=TIPRE2 PE=3 SV=1	[more]
A0A0A7HIT2	1.78e-17	53.66	TIPRE4 (Fragment) OS=Momordica anigosantha OX=703354 GN=TIPRE4 PE=3 SV=1	[more]
A0A0A7HIA9	5.64e-17	51.81	TIPRE5 (Fragment) OS=Momordica friesiorum OX=703365 GN=TIPRE5 PE=3 SV=1	[more]

BLAST of MC10g0355 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SMART	SM00286	PTI_1	coord: 65..93 e-value: 4.4E-13 score: 59.5
None	No IPR available	GENE3D	4.10.75.20		coord: 44..93 e-value: 4.4E-15 score: 57.2
IPR000737	Proteinase inhibitor I7, squash	PFAM	PF00299	Squash	coord: 65..93 e-value: 2.5E-15 score: 56.2
IPR000737	Proteinase inhibitor I7, squash	PROSITE	PS00286	SQUASH_INHIBITOR	coord: 67..86
IPR000737	Proteinase inhibitor I7, squash	CDD	cd00150	PlantTI	coord: 67..93 e-value: 5.65614E-12 score: 53.1503
IPR011052	Proteinase/amylase inhibitor domain superfamily	SUPERFAMILY	57027	Plant inhibitors of proteinases and amylases	coord: 62..93

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC10g0355.1	MC10g0355.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0110165	cellular anatomical entity
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity
molecular_function	GO:0004866	endopeptidase inhibitor activity