MC09g1753 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g1753
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionU4/U6 small nuclear ribonucleoprotein Prp31 homolog
LocationMC09: 22755157 .. 22759424 (+)
RNA-Seq ExpressionMC09g1753
SyntenyMC09g1753
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GCCCATTACATTCTCTCCCTCATTTAAAAGTTAAATCAAAGCCCAGCCCATTTGACAACCACCCTATCTTCGTCTCTCTCCGATCTGGTGTGAGGACGTCACGGGCTCGTCGAATCGCTGCTTGGAGAACGCACCGCCTCTCGTGGTAATCGCCGTGGAGAGTCGCCGACGCTCGGGTCTGACTTCGCGTGGGTTCGCCGGAGGGTTCTGCACCGCCGCGAGGTGATGCCGCCGACTTCCTATACATCGCGCCACCGTCAGTAGTTTACCGTGTTCGATCGTCGTTAGGTATTTTCTTTTTCTTTTTCTTTGGGATAAAGGCATCGGAGTGTGATTTTATACATTAGGTTTGTGTATATATATGCTCCCTTGACCATGGAAATTGAAATTTTGTTATTCAGCTATTCTTCAACTTGGTGCTGAATTGTTTACGACCAGGAAGGCTTTGTAATCTTCTAGCGCAGTTTAGAAAATGGTCAGTAACCTATTCTCTCTTTGGTTTGCGGCTCTGACACGTTTTGATTTCAGCACTCTCTTCTGAGTTAAGAGTGCTCATTAGTTTTCAGTTTTATTACTGTTAATTAGTTACTAATTCCTGCTGTTAATTAGTTGTATTACTGTTGTAAGAGTGTTCTGGTTCGAAGCCTTTAGATCATCTCCCTCAGTGTTTGATAAAATCACCATTGTTCTACTGCTTCTCACGAGCGATTGAAAAACAAATCGTGCCCTAGTCGCTAGACTCATAGCTCTGATACCATGGCCATCAAAATTAAAAGAGAATAGGACAATGACAAATGCAGAGAAATAGTACTAATATTCTCTTTAATCGAGAATTAGTGAAGAGTACGCCGAATACAGCCTATTATAGGCATGGGTAGTTCTGCACTATTATCTGACTGATTATCAGCCCTTTACAACAGTAATACAACTAATTATCAACAGTAATTAGTAACTAATTAACAGCAATGAAACTGAAAACTAATGAGCACTCGTAACTTTCTGTATGCTGGTCTGTATCAGTTGCTATGATTTTGCGTGTAAACTTGAGTTGACTTATCTACTAGACATTGTGGGTTTGGGAATATATCATTGAATCTGTAGCTCAGTTCTTAGTCTTCTGCTGATGCTATGTTGCTGTCGTTCTTCCTTCAATTGTATAATTGCGTAAGGGATATCTATATAATTGAACAAGTTGAGAATCGTTTCTTTTGTTTCTACTTTATTTTAAAGCATTTATTTTTTATCTTTTTTATTAGGCATTTCAACTTGACATGACGCTACCTCTTTCCGTGATTTATTAGAATCTATCTTGAAAATGTTAATTATCTTGATTATTAACTGTCGTATTCTTTTCTTGTGTTTTGTAGGCTACTTTGGCTGATTCTTTCCTAGCAGATCTTGATGAACTCTCTGATGAAGACAATTTTCTGGTAAGTAATGAATTTTACTTTCCTTTCTGGAAAGCAAAGTCATAATAATGCTATTAGTAGTGTAAATCCATCTACCTTATATTTTGAGGCAATAGTTATTGGCTGTCTACAGGATGAAGAAGATGTCGATGCTGAAAATATGGAAGAGGATATTGATGGGGACCTTGCCGACCTAGAAAGCCTTAATTATGATGATCTGGATAGTGTATCAAAATTGCAGAAAACGCAGAGATACAATGATATAATGCAGGTTAGTTTACATGATAATTTCCTTATTGATTAGGGCATATGCTGTGGCTTGAAGATAAAAGGGTTGGAAGAAACCCAATGCATGCGTTGGATCCCTGCTGTCTTGTCTATTTTTTAATCTTTTATTGAATGATAAGTTATGGCTTATGGGTTATTGTAAAATTGTTGGATGTGTCTTCTTGGCAATTCATTGTTTCTAAATAATATTACATTTGAAGATTGTGAAAATCTATGCAAGTTATGATGACCGTTTCAATTTTACTATCCACAACAATGATGTTACTTGAACCATAACTCCTCTATTTGAAATGTTAAATATTTTAGTGTGCATACATGATATTATTTGTGATGCCTCATTTCCAAGTCACTAACTTTTCTGTTGCCATCTGAATGACAGAAAGTGGAAGGTGCATTGCAGAAGGATTCTAACATCTCCAATCAAGGATTAGTCTTGGAAGATGATCCTGAGTACCAACTGATTGTGGAGTGCAACTCTCTGTCAGTGGATATTGAGAATGAAATTATTATTATTCACAATTTTATACGTGATAAATATCGATTGAAATTTCCAGAGCTTGAATCACTTGTGCACCACCCAATCGATTATGCTCGAGTTGTTAAAAAGATTGGGAACGAAATGGATTTGACTCTTGTTGATCTAGAAGGCCTTTTACCCTCTGCTGTCATTATGGTTGTCTCAGTTACTGCATCCACCACAAGTGGCAAGCCGCTTCCAGAAGAAATTCTCCAGAAAACAATTGATGCATGTGACCGAGCTCTTGCTTTAGATTCAGCAAAGAAAAAGGTCCTTAATTTTGTTGAAAGTAGAATGGGATATATTGCACCAAATCTTTCAGCCATAGTTGGAAGTGCTGTTGCAGCAAAGCTAATGGGAACTGCTGGTGGTCTTGCTGCTTTAGCTAAGATGCCTGCTTGCAATGTTCAGCTTCTTGGTGCAAAGAAAAAAAATCTTGCTGGGTTTTCCACAGCGACCTCACAGTTTCGGGTAGGTTATATTGAGCAAACAGAGATATTCCAATCAACACCTCCTCCTTTGAAGATGCGTGCTTGTCGACTCATAGCTGCAAAATCAACACTTGCAGCACGTGTTGACTCTACCAGGGGGGATCCTACTGGGAAGACTGGAAGAGTTTTCAAAGACGAGATCCTTAAGAAAATTGAGAAGTGGCAAGAACCTCCACCTGCAAAACAACCAAAACCTCTTCCAGTCCCTGATTCAGAACCCAAAAAGAAGAGAGGTGGGCGTCGACTAAGGAAGATGAAGGAAAGGTACTGATATGTTACATGTGTCTTTCATCTCTCTAACTATAGAAGCTTTGATTTGTTGCAGTTTTTGGTGTTCATGGATTGATGTGATTTTCCTTGTCAGATATGCAACAACAGAGATGAGGAAGCTAGCCAACAGAATGCAGTTTGGGGTGCCTGAAGAGAGTTCTTTAGGTAAAGCATTCTTTAAATTTTGAGTAATCTGGATAGATGAAATTTTAGTCCTGCTTCTGCTTCATTTCACATTACATGTCAGTATGGATGCAATGTTGGGTTGTTCAATAAGCATGAGTTGCCAAGTTGTCTGGTGAACCAGTAAATTTTCTGAATTGCAAGTAACACTACCATAACCGTCTTTCCCTCTCTTGAAGAAATATTTCATATCAGAATTGGTTTTATTTTCTTCTGAATACCAGCATATTTAGAAATCTGTTCTGGATGGAAAAAGTAGTTGTTTGAGCATTCTGCAATAATATTCTGCACAAACATTTCACTTTCTTCATTCTTTCTGCACATAAAACAGGAGATGGATTAGGGGAAGGGTAGAAATCTGTTCTGGATGGAAAAAGTAGTTGTTTGAGCATTCTGCAATAATATTCTGCACAAACATTTCACTTTCTTCATTCTTTCTGCACATAAAACAGGAGATGGATTAGGGGAAGGGTAGAAATCTGTTCTGGATGGAAAAAGTAGTTGTTTGAGCATTCTGCAATAATATTCTGCACAAACATTTCACTTTCTTCATTCTTTCTGCACATAAAACAGGAGATGGATTAGGGGAAGGGTATGGAATGCTTGGGCAGGCTGGGAGTGGCAAGCTGCGTGTGTCAGCTGCTCAAAGCAAGCTTGCCGCTAAAGTTGTTAAGAAGTAAGTCATGGCATTGAGCTGGTCCTAATATCTTTCACACACAAAATCCTCCCCACCACAGATATCTGCAGCTCACTATTGGGTTATTTTTTATATTTTCTTTCTAAATTATTTTCAGGTTCAAGGAAAAACGTTATGGTAGCAGTGGTGCTACATCCGGGCTGACATCAAGTTTAGCATTTACACCTGTACAAGTCGGTATTTCTTGCGTTCCTCACCATATCTTCCTATGTTATCTAAGTTAGCCTCAACACAAGTAATTGTCTTTTTCATGACACTGGCCAGATGCTTAATTTCTAGGTTGTAAGGATTTCCTGTTTTCCTTGCAGGGGATTGAACTGTCGAATCCTCAGGCTCATTTGAACCAGCTAGGCAGTGGAACTCAGAGCACCTACTTTTCCGAAACAGGAACATTTTCAAAGATCAGGAAAAAC

mRNA sequence

GCCCATTACATTCTCTCCCTCATTTAAAAGTTAAATCAAAGCCCAGCCCATTTGACAACCACCCTATCTTCGTCTCTCTCCGATCTGGTGTGAGGACGTCACGGGCTCGTCGAATCGCTGCTTGGAGAACGCACCGCCTCTCGTGGTAATCGCCGTGGAGAGTCGCCGACGCTCGGGTCTGACTTCGCGTGGGTTCGCCGGAGGGTTCTGCACCGCCGCGAGGTGATGCCGCCGACTTCCTATACATCGCGCCACCGTCAGTAGTTTACCGTGTTCGATCGTCGTTAGCTATTCTTCAACTTGGTGCTGAATTGTTTACGACCAGGAAGGCTTTGTAATCTTCTAGCGCAGTTTAGAAAATGGCTACTTTGGCTGATTCTTTCCTAGCAGATCTTGATGAACTCTCTGATGAAGACAATTTTCTGGATGAAGAAGATGTCGATGCTGAAAATATGGAAGAGGATATTGATGGGGACCTTGCCGACCTAGAAAGCCTTAATTATGATGATCTGGATAGTGTATCAAAATTGCAGAAAACGCAGAGATACAATGATATAATGCAGAAAGTGGAAGGTGCATTGCAGAAGGATTCTAACATCTCCAATCAAGGATTAGTCTTGGAAGATGATCCTGAGTACCAACTGATTGTGGAGTGCAACTCTCTGTCAGTGGATATTGAGAATGAAATTATTATTATTCACAATTTTATACGTGATAAATATCGATTGAAATTTCCAGAGCTTGAATCACTTGTGCACCACCCAATCGATTATGCTCGAGTTGTTAAAAAGATTGGGAACGAAATGGATTTGACTCTTGTTGATCTAGAAGGCCTTTTACCCTCTGCTGTCATTATGGTTGTCTCAGTTACTGCATCCACCACAAGTGGCAAGCCGCTTCCAGAAGAAATTCTCCAGAAAACAATTGATGCATGTGACCGAGCTCTTGCTTTAGATTCAGCAAAGAAAAAGGTCCTTAATTTTGTTGAAAGTAGAATGGGATATATTGCACCAAATCTTTCAGCCATAGTTGGAAGTGCTGTTGCAGCAAAGCTAATGGGAACTGCTGGTGGTCTTGCTGCTTTAGCTAAGATGCCTGCTTGCAATGTTCAGCTTCTTGGTGCAAAGAAAAAAAATCTTGCTGGGTTTTCCACAGCGACCTCACAGTTTCGGGTAGGTTATATTGAGCAAACAGAGATATTCCAATCAACACCTCCTCCTTTGAAGATGCGTGCTTGTCGACTCATAGCTGCAAAATCAACACTTGCAGCACGTGTTGACTCTACCAGGGGGGATCCTACTGGGAAGACTGGAAGAGTTTTCAAAGACGAGATCCTTAAGAAAATTGAGAAGTGGCAAGAACCTCCACCTGCAAAACAACCAAAACCTCTTCCAGTCCCTGATTCAGAACCCAAAAAGAAGAGAGGTGGGCGTCGACTAAGGAAGATGAAGGAAAGATATGCAACAACAGAGATGAGGAAGCTAGCCAACAGAATGCAGTTTGGGGTGCCTGAAGAGAGTTCTTTAGGAGATGGATTAGGGGAAGGGTATGGAATGCTTGGGCAGGCTGGGAGTGGCAAGCTGCGTGTGTCAGCTGCTCAAAGCAAGCTTGCCGCTAAAGTTGTTAAGAAGTTCAAGGAAAAACGTTATGGTAGCAGTGGTGCTACATCCGGGCTGACATCAAGTTTAGCATTTACACCTGTACAAGGGATTGAACTGTCGAATCCTCAGGCTCATTTGAACCAGCTAGGCAGTGGAACTCAGAGCACCTACTTTTCCGAAACAGGAACATTTTCAAAGATCAGGAAAAAC

Coding sequence (CDS)

ATGGCTACTTTGGCTGATTCTTTCCTAGCAGATCTTGATGAACTCTCTGATGAAGACAATTTTCTGGATGAAGAAGATGTCGATGCTGAAAATATGGAAGAGGATATTGATGGGGACCTTGCCGACCTAGAAAGCCTTAATTATGATGATCTGGATAGTGTATCAAAATTGCAGAAAACGCAGAGATACAATGATATAATGCAGAAAGTGGAAGGTGCATTGCAGAAGGATTCTAACATCTCCAATCAAGGATTAGTCTTGGAAGATGATCCTGAGTACCAACTGATTGTGGAGTGCAACTCTCTGTCAGTGGATATTGAGAATGAAATTATTATTATTCACAATTTTATACGTGATAAATATCGATTGAAATTTCCAGAGCTTGAATCACTTGTGCACCACCCAATCGATTATGCTCGAGTTGTTAAAAAGATTGGGAACGAAATGGATTTGACTCTTGTTGATCTAGAAGGCCTTTTACCCTCTGCTGTCATTATGGTTGTCTCAGTTACTGCATCCACCACAAGTGGCAAGCCGCTTCCAGAAGAAATTCTCCAGAAAACAATTGATGCATGTGACCGAGCTCTTGCTTTAGATTCAGCAAAGAAAAAGGTCCTTAATTTTGTTGAAAGTAGAATGGGATATATTGCACCAAATCTTTCAGCCATAGTTGGAAGTGCTGTTGCAGCAAAGCTAATGGGAACTGCTGGTGGTCTTGCTGCTTTAGCTAAGATGCCTGCTTGCAATGTTCAGCTTCTTGGTGCAAAGAAAAAAAATCTTGCTGGGTTTTCCACAGCGACCTCACAGTTTCGGGTAGGTTATATTGAGCAAACAGAGATATTCCAATCAACACCTCCTCCTTTGAAGATGCGTGCTTGTCGACTCATAGCTGCAAAATCAACACTTGCAGCACGTGTTGACTCTACCAGGGGGGATCCTACTGGGAAGACTGGAAGAGTTTTCAAAGACGAGATCCTTAAGAAAATTGAGAAGTGGCAAGAACCTCCACCTGCAAAACAACCAAAACCTCTTCCAGTCCCTGATTCAGAACCCAAAAAGAAGAGAGGTGGGCGTCGACTAAGGAAGATGAAGGAAAGATATGCAACAACAGAGATGAGGAAGCTAGCCAACAGAATGCAGTTTGGGGTGCCTGAAGAGAGTTCTTTAGGAGATGGATTAGGGGAAGGGTATGGAATGCTTGGGCAGGCTGGGAGTGGCAAGCTGCGTGTGTCAGCTGCTCAAAGCAAGCTTGCCGCTAAAGTTGTTAAGAAGTTCAAGGAAAAACGTTATGGTAGCAGTGGTGCTACATCCGGGCTGACATCAAGTTTAGCATTTACACCTGTACAAGGGATTGAACTGTCGAATCCTCAGGCTCATTTGAACCAGCTAGGCAGTGGAACTCAGAGCACCTACTTTTCCGAAACAGGAACATTTTCAAAGATCAGGAAAAAC

Protein sequence

MATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKTQRYNDIMQKVEGALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDKYRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLPEEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLAALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKSTLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLRKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFSKIRKN
Homology
BLAST of MC09g1753 vs. ExPASy Swiss-Prot
Match: Q8RXN6 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog OS=Arabidopsis thaliana OX=3702 GN=PRP31 PE=1 SV=1)

HSP 1 Score: 697.2 bits (1798), Expect = 1.3e-199
Identity = 376/485 (77.53%), Postives = 421/485 (86.80%), Query Frame = 0

Query: 1   MATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKT 60
           MATL DSFLADLDELSD +  LDE D D    EED+D D+ADLE+LNYDDLD+VSKLQK+
Sbjct: 1   MATLEDSFLADLDELSDNEAELDENDGDVGKEEEDVDMDMADLETLNYDDLDNVSKLQKS 60

Query: 61  QRYNDIMQKVEGALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDK 120
           QRY DIM KVE AL KDS+ + +G VLEDDPEY+LIV+CN LSVDIENEI+I+HNFI+DK
Sbjct: 61  QRYADIMHKVEEALGKDSDGAEKGTVLEDDPEYKLIVDCNQLSVDIENEIVIVHNFIKDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           Y+LKF ELESLVHHPIDYA VVKKIGNE DL LVDL  LLPSA+IMVVSVTA TT G  L
Sbjct: 121 YKLKFQELESLVHHPIDYACVVKKIGNETDLALVDLADLLPSAIIMVVSVTALTTKGSAL 180

Query: 181 PEEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240
           PE++LQK ++ACDRAL LDSA+KKVL FVES+MG IAPNLSAIVGSAVAAKLMGTAGGL+
Sbjct: 181 PEDVLQKVLEACDRALDLDSARKKVLEFVESKMGSIAPNLSAIVGSAVAAKLMGTAGGLS 240

Query: 241 ALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKS 300
           ALAKMPACNVQ+LG K+KNLAGFS+ATSQ RVGY+EQTEI+QSTPP L+ RA RL+AAKS
Sbjct: 241 ALAKMPACNVQVLGHKRKNLAGFSSATSQSRVGYLEQTEIYQSTPPGLQARAGRLVAAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVD+TRGDP G +G+ F++EI KKIEKWQEPPPA+QPKPLPVPDSEPKK+RGGRRL
Sbjct: 301 TLAARVDATRGDPLGISGKAFREEIRKKIEKWQEPPPARQPKPLPVPDSEPKKRRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLA-- 420
           RKMKERY  T+MRKLANRM FG PEESSLGDGLGEGYGMLGQAGS +LRVS+  SKL   
Sbjct: 361 RKMKERYQVTDMRKLANRMAFGTPEESSLGDGLGEGYGMLGQAGSNRLRVSSVPSKLKIN 420

Query: 421 AKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTF 480
           AKV KK KE++Y     TSGLTSSLAFTPVQGIEL NPQ  L  LGSGTQSTYFSE+GTF
Sbjct: 421 AKVAKKLKERQYAGGATTSGLTSSLAFTPVQGIELCNPQQALG-LGSGTQSTYFSESGTF 480

Query: 481 SKIRK 484
           SK++K
Sbjct: 481 SKLKK 484

BLAST of MC09g1753 vs. ExPASy Swiss-Prot
Match: Q5U5C5 (U4/U6 small nuclear ribonucleoprotein Prp31 OS=Xenopus laevis OX=8355 GN=prpf31 PE=2 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 7.6e-107
Identity = 235/495 (47.47%), Postives = 330/495 (66.67%), Query Frame = 0

Query: 3   TLADSFLADLDELS--DEDNFLDEEDVDA-ENMEEDIDGDLADLESLNYDDLDSVSKLQK 62
           +LAD  LADL+E +  +E+N +DE+D++  E ++E++  D      LN + + S++KL  
Sbjct: 2   SLADELLADLEEAAEEEEENLIDEDDLETIEEVDEEMQVD------LNAESVKSIAKLSD 61

Query: 63  TQRYNDIMQKVEGALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRD 122
           ++ +++I+ K+EG +QK    S     +E  PEY++IV+ N+L+V+IENE+ IIH FIRD
Sbjct: 62  SKLFSEILLKIEGYIQKQPKASEVMGPVEAAPEYKVIVDANNLTVEIENELNIIHKFIRD 121

Query: 123 KYRLKFPELESLVHHPIDYARVVKKIGNEMD--LTLVDLEGLLPSAVIMVVSVTASTTSG 182
           KY  +FPELESLV + +DY R VK++GN +D      +L+ +L +A IMVVSVTASTT G
Sbjct: 122 KYSKRFPELESLVPNALDYIRTVKELGNNLDKCKNNENLQQILTNATIMVVSVTASTTQG 181

Query: 183 KPLPEEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAG 242
           + L +E L++  +ACD AL L+ +K ++  +VESRM +IAPNLS IVG++ AAK+MG AG
Sbjct: 182 QQLTDEELERIEEACDMALELNQSKHRIYEYVESRMSFIAPNLSIIVGASTAAKIMGIAG 241

Query: 243 GLAALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIA 302
           GL  L+KMPACNV LLGA++K L GFS+ +     GYI  +EI QS P  L  +A RL++
Sbjct: 242 GLTNLSKMPACNVMLLGAQRKTLTGFSSTSVLPHTGYIYHSEIVQSLPSDLHRKAARLVS 301

Query: 303 AKSTLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGG 362
           AK TLA+RVDS   +P GK G   K+EI +K +KWQEPPP KQ KPLP P    +KKRGG
Sbjct: 302 AKCTLASRVDSFHENPEGKIGYDLKEEIERKFDKWQEPPPVKQVKPLPAPLDGQRKKRGG 361

Query: 363 RRLRKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLR---VSAAQ 422
           RR RKMKER   TE+RK ANRM FG  EE +  + LG   G LG++GSG++R   V+ A 
Sbjct: 362 RRYRKMKERLGLTEIRKQANRMSFGEIEEDAYQEDLGFSLGHLGKSGSGRIRQAQVNEAT 421

Query: 423 SKLAAKVVKKFKEKR---YGSSGA----TSGLTSSLAFTPVQGIELSNPQAHLNQLGSGT 482
               +K +++  +K+   YG        +SG  SS+AFTP+QG+E+ NPQA   ++    
Sbjct: 422 KARISKTLQRTLQKQSVVYGGKSTVRDRSSGTASSVAFTPLQGLEIVNPQAAEKKVAEAN 481

BLAST of MC09g1753 vs. ExPASy Swiss-Prot
Match: Q8CCF0 (U4/U6 small nuclear ribonucleoprotein Prp31 OS=Mus musculus OX=10090 GN=Prpf31 PE=1 SV=3)

HSP 1 Score: 386.0 bits (990), Expect = 6.5e-106
Identity = 234/496 (47.18%), Postives = 331/496 (66.73%), Query Frame = 0

Query: 3   TLADSFLADLDELSDED---NFLDEEDVDA-ENMEEDIDGDLADLESLNYDDLDSVSKLQ 62
           +LAD  LADL+E ++E+   ++ +EE+  A E+++E+   DL+       D + S++KL 
Sbjct: 2   SLADELLADLEEAAEEEEGGSYGEEEEEPAIEDVQEETQLDLSG------DSVKSIAKLW 61

Query: 63  KTQRYNDIMQKVEGALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIR 122
            ++ + +IM K+E  + K +N+S     +E  PEY++IV+ N+L+V+IENE+ IIH FIR
Sbjct: 62  DSKMFAEIMMKIEEYISKQANVSEVMGPVEAAPEYRVIVDANNLTVEIENELNIIHKFIR 121

Query: 123 DKYRLKFPELESLVHHPIDYARVVKKIGNEMD--LTLVDLEGLLPSAVIMVVSVTASTTS 182
           DKY  +FPELESLV + +DY R VK++GN +D      +L+ +L +A IMVVSVTASTT 
Sbjct: 122 DKYSKRFPELESLVPNALDYIRTVKELGNSLDKCKNNENLQQILTNATIMVVSVTASTTQ 181

Query: 183 GKPLPEEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTA 242
           G+ L +E L++  +ACD AL L+++K ++  +VESRM +IAPNLS I+G++ AAK+MG A
Sbjct: 182 GQQLSDEELERLEEACDMALELNASKHRIYEYVESRMSFIAPNLSIIIGASTAAKIMGVA 241

Query: 243 GGLAALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLI 302
           GGL  L+KMPACN+ LLGA++K L+GFS+ +     GYI  ++I QS PP L+ +A RL+
Sbjct: 242 GGLTNLSKMPACNIMLLGAQRKTLSGFSSTSVLPHTGYIYHSDIVQSLPPDLRRKAARLV 301

Query: 303 AAKSTLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRG 362
           AAK TLAARVDS      GK G   KDEI +K +KWQEPPP KQ KPLP P    +KKRG
Sbjct: 302 AAKCTLAARVDSFHESTEGKVGYELKDEIERKFDKWQEPPPVKQVKPLPAPLDGQRKKRG 361

Query: 363 GRRLRKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLR---VSAA 422
           GRR RKMKER   TE+RK ANRM FG  EE +  + LG   G LG++GSG++R   V+ A
Sbjct: 362 GRRYRKMKERLGLTEIRKQANRMSFGEIEEDAYQEDLGFSLGHLGKSGSGRVRQTQVNEA 421

Query: 423 QSKLAAKVVKKFKEKR---YGSSGA----TSGLTSSLAFTPVQGIELSNPQAHLNQLGSG 482
                +K +++  +K+   YG        +SG  SS+AFTP+QG+E+ NPQA   ++   
Sbjct: 422 TKARISKTLQRTLQKQSVVYGGKSTIRDRSSGTASSVAFTPLQGLEIVNPQAAEKKVAEA 481

BLAST of MC09g1753 vs. ExPASy Swiss-Prot
Match: Q6NVP6 (U4/U6 small nuclear ribonucleoprotein Prp31 OS=Xenopus tropicalis OX=8364 GN=prpf31 PE=2 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 1.9e-105
Identity = 232/495 (46.87%), Postives = 329/495 (66.46%), Query Frame = 0

Query: 3   TLADSFLADLDELS--DEDNFLDEEDVDA-ENMEEDIDGDLADLESLNYDDLDSVSKLQK 62
           +LAD  LADL+E +  +E+N +DE+D++  E ++E++  D      LN + + S++KL  
Sbjct: 2   SLADELLADLEEAAEEEEENLIDEDDLETIEEVQEEMQVD------LNAESVKSIAKLSD 61

Query: 63  TQRYNDIMQKVEGALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRD 122
           ++ +++I+ K++G ++K    S     +E  PEY++IV+ N+L+V+IENE+ IIH FIRD
Sbjct: 62  SKLFSEILLKIDGYIKKQPKASEVMGPVEAAPEYKVIVDANNLTVEIENELNIIHKFIRD 121

Query: 123 KYRLKFPELESLVHHPIDYARVVKKIGNEMD--LTLVDLEGLLPSAVIMVVSVTASTTSG 182
           KY  +FPELESLV + +DY R VK++GN +D      +L+ +L +A IMVVSVTASTT G
Sbjct: 122 KYSKRFPELESLVPNALDYIRTVKELGNNLDKCKNNENLQQILTNATIMVVSVTASTTQG 181

Query: 183 KPLPEEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAG 242
           + L +E L++  +ACD AL L+ +K ++  +VESRM +IAPNLS IVG++ AAK+MG AG
Sbjct: 182 QQLTDEELERIEEACDMALELNQSKHRIYEYVESRMSFIAPNLSIIVGASTAAKIMGIAG 241

Query: 243 GLAALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIA 302
           GL  L+KMPACNV LLGA++K L+GFS+ +     GYI  ++I QS PP L  +A RL++
Sbjct: 242 GLTNLSKMPACNVMLLGAQRKTLSGFSSTSVLPHTGYIYHSDIVQSLPPDLHRKAARLVS 301

Query: 303 AKSTLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGG 362
           AK TLAARVDS      GK G   K+EI +K +KWQEPPP KQ KPLP P    +KKRGG
Sbjct: 302 AKCTLAARVDSFHESSEGKVGYDLKEEIERKFDKWQEPPPVKQVKPLPAPLDGQRKKRGG 361

Query: 363 RRLRKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLR---VSAAQ 422
           RR RKMKER   TE+RK ANRM F   EE +  + LG   G LG++GSG++R   V+ A 
Sbjct: 362 RRYRKMKERLGLTEIRKQANRMSFAEIEEDAYQEDLGFSLGHLGKSGSGRIRQAQVNEAT 421

Query: 423 SKLAAKVVKKFKEKR---YGSSGA----TSGLTSSLAFTPVQGIELSNPQAHLNQLGSGT 482
               +K +++  +K+   YG        +SG  SS+AFTP+QG+E+ NPQA   ++    
Sbjct: 422 KARISKTLQRTLQKQSVVYGGKSTIRDRSSGTASSVAFTPLQGLEIVNPQAAEKKVAEAN 481

BLAST of MC09g1753 vs. ExPASy Swiss-Prot
Match: Q8WWY3 (U4/U6 small nuclear ribonucleoprotein Prp31 OS=Homo sapiens OX=9606 GN=PRPF31 PE=1 SV=2)

HSP 1 Score: 382.1 bits (980), Expect = 9.3e-105
Identity = 233/496 (46.98%), Postives = 329/496 (66.33%), Query Frame = 0

Query: 3   TLADSFLADLDELSDED---NFLDEEDVDA-ENMEEDIDGDLADLESLNYDDLDSVSKLQ 62
           +LAD  LADL+E ++E+   ++ +EE+  A E+++E+   DL+       D + +++KL 
Sbjct: 2   SLADELLADLEEAAEEEEGGSYGEEEEEPAIEDVQEETQLDLSG------DSVKTIAKLW 61

Query: 63  KTQRYNDIMQKVEGALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIR 122
            ++ + +IM K+E  + K +  S     +E  PEY++IV+ N+L+V+IENE+ IIH FIR
Sbjct: 62  DSKMFAEIMMKIEEYISKQAKASEVMGPVEAAPEYRVIVDANNLTVEIENELNIIHKFIR 121

Query: 123 DKYRLKFPELESLVHHPIDYARVVKKIGNEMD--LTLVDLEGLLPSAVIMVVSVTASTTS 182
           DKY  +FPELESLV + +DY R VK++GN +D      +L+ +L +A IMVVSVTASTT 
Sbjct: 122 DKYSKRFPELESLVPNALDYIRTVKELGNSLDKCKNNENLQQILTNATIMVVSVTASTTQ 181

Query: 183 GKPLPEEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTA 242
           G+ L EE L++  +ACD AL L+++K ++  +VESRM +IAPNLS I+G++ AAK+MG A
Sbjct: 182 GQQLSEEELERLEEACDMALELNASKHRIYEYVESRMSFIAPNLSIIIGASTAAKIMGVA 241

Query: 243 GGLAALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLI 302
           GGL  L+KMPACN+ LLGA++K L+GFS+ +     GYI  ++I QS PP L+ +A RL+
Sbjct: 242 GGLTNLSKMPACNIMLLGAQRKTLSGFSSTSVLPHTGYIYHSDIVQSLPPDLRRKAARLV 301

Query: 303 AAKSTLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRG 362
           AAK TLAARVDS      GK G   KDEI +K +KWQEPPP KQ KPLP P    +KKRG
Sbjct: 302 AAKCTLAARVDSFHESTEGKVGYELKDEIERKFDKWQEPPPVKQVKPLPAPLDGQRKKRG 361

Query: 363 GRRLRKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLR---VSAA 422
           GRR RKMKER   TE+RK ANRM FG  EE +  + LG   G LG++GSG++R   V+ A
Sbjct: 362 GRRYRKMKERLGLTEIRKQANRMSFGEIEEDAYQEDLGFSLGHLGKSGSGRVRQTQVNEA 421

Query: 423 QSKLAAKVVKKFKEKR---YGSSGA----TSGLTSSLAFTPVQGIELSNPQAHLNQLGSG 482
                +K +++  +K+   YG        +SG  SS+AFTP+QG+E+ NPQA   ++   
Sbjct: 422 TKARISKTLQRTLQKQSVVYGGKSTIRDRSSGTASSVAFTPLQGLEIVNPQAAEKKVAEA 481

BLAST of MC09g1753 vs. NCBI nr
Match: XP_022151138.1 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 [Momordica charantia])

HSP 1 Score: 905 bits (2339), Expect = 0.0
Identity = 484/484 (100.00%), Postives = 484/484 (100.00%), Query Frame = 0

Query: 1   MATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKT 60
           MATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKT
Sbjct: 1   MATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKT 60

Query: 61  QRYNDIMQKVEGALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDK 120
           QRYNDIMQKVEGALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDK
Sbjct: 61  QRYNDIMQKVEGALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL
Sbjct: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180

Query: 181 PEEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240
           PEEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA
Sbjct: 181 PEEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240

Query: 241 ALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKS 300
           ALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKS
Sbjct: 241 ALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL
Sbjct: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420
           RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK
Sbjct: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420

Query: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFSK 480
           VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFSK
Sbjct: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFSK 480

Query: 481 IRKN 484
           IRKN
Sbjct: 481 IRKN 484

BLAST of MC09g1753 vs. NCBI nr
Match: XP_022945419.1 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog [Cucurbita moschata] >XP_022945420.1 U4/U6 small nuclear ribonucleoprotein Prp31 homolog [Cucurbita moschata] >KAG6573747.1 U4/U6 small nuclear ribonucleoprotein Prp31-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 886 bits (2289), Expect = 0.0
Identity = 472/484 (97.52%), Postives = 479/484 (98.97%), Query Frame = 0

Query: 1   MATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKT 60
           MATLADSFLADLDELSDEDNF DEEDVDAENMEEDIDGDLADLESLNYDDLD+VSKLQK+
Sbjct: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60

Query: 61  QRYNDIMQKVEGALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDK 120
           QRY+DIMQKVE ALQKDSNISNQGLVLEDDPEYQLIVECN+LSVDIENEIIIIHNFIRDK
Sbjct: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL
Sbjct: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180

Query: 181 PEEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240
           PEEILQKTIDACDRALALDSAKK VLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGL 
Sbjct: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLG 240

Query: 241 ALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKS 300
           +LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRL++AKS
Sbjct: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLVSAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL
Sbjct: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420
           RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK
Sbjct: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420

Query: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFSK 480
           VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLG GTQSTYFSETGTFSK
Sbjct: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGGGTQSTYFSETGTFSK 480

Query: 481 IRKN 484
           IRKN
Sbjct: 481 IRKN 484

BLAST of MC09g1753 vs. NCBI nr
Match: XP_022956658.1 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 [Cucurbita moschata] >XP_023004221.1 U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 [Cucurbita maxima] >XP_023526610.1 U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 [Cucurbita pepo subsp. pepo] >KAG6601489.1 U4/U6 small nuclear ribonucleoprotein Prp31-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 885 bits (2287), Expect = 0.0
Identity = 472/484 (97.52%), Postives = 475/484 (98.14%), Query Frame = 0

Query: 1   MATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKT 60
           MATLADSFLADLDELSDEDNFLDEED DAENMEEDIDGDLADLESLNYDDLDSVSKLQKT
Sbjct: 1   MATLADSFLADLDELSDEDNFLDEEDADAENMEEDIDGDLADLESLNYDDLDSVSKLQKT 60

Query: 61  QRYNDIMQKVEGALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDK 120
           QRYNDIMQKV  AL+ DSN+SNQG VLEDDPEYQLIVECN+LSVDIENEIIIIHNFIRDK
Sbjct: 61  QRYNDIMQKVADALEMDSNVSNQGFVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL
Sbjct: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180

Query: 181 PEEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240
           PEEILQKTIDACDRAL LDSAKK VLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGL 
Sbjct: 181 PEEILQKTIDACDRALTLDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLV 240

Query: 241 ALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKS 300
           ALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKS
Sbjct: 241 ALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL
Sbjct: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420
           RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK
Sbjct: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420

Query: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFSK 480
           VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLG GTQSTYFSETGTFSK
Sbjct: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGGGTQSTYFSETGTFSK 480

Query: 481 IRKN 484
           IRKN
Sbjct: 481 IRKN 484

BLAST of MC09g1753 vs. NCBI nr
Match: XP_022966885.1 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog [Cucurbita maxima])

HSP 1 Score: 884 bits (2283), Expect = 0.0
Identity = 471/484 (97.31%), Postives = 480/484 (99.17%), Query Frame = 0

Query: 1   MATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKT 60
           MATLADSFLADLDELSDEDNF DEEDVDAENMEEDIDGDLADLESLNYDDLD+VSKLQK+
Sbjct: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60

Query: 61  QRYNDIMQKVEGALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDK 120
           QRY+DIMQKVE ALQKDSNISNQGLVLEDDPEYQLIVECN+LSVDIENEIIIIHNFIRDK
Sbjct: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL
Sbjct: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180

Query: 181 PEEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240
           PEEILQKTIDACDRALALDSAKK VLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA
Sbjct: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240

Query: 241 ALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKS 300
           +LAKMPACNVQLLGAKKKNLAGFSTATSQFR+GYIEQTEIFQSTPP LKMRACRL++AKS
Sbjct: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRLGYIEQTEIFQSTPPHLKMRACRLVSAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL
Sbjct: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420
           RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK
Sbjct: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420

Query: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFSK 480
           VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAH+NQLGSGTQSTYFSETGTFSK
Sbjct: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHMNQLGSGTQSTYFSETGTFSK 480

Query: 481 IRKN 484
           IRKN
Sbjct: 481 IRKN 484

BLAST of MC09g1753 vs. NCBI nr
Match: KAG7032269.1 (U4/U6 small nuclear ribonucleoprotein Prp31-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 883 bits (2282), Expect = 0.0
Identity = 471/483 (97.52%), Postives = 474/483 (98.14%), Query Frame = 0

Query: 2   ATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKTQ 61
           ATLADSFLADLDELSDEDNFLDEED DAENMEEDIDGDLADLESLNYDDLDSVSKLQKTQ
Sbjct: 64  ATLADSFLADLDELSDEDNFLDEEDADAENMEEDIDGDLADLESLNYDDLDSVSKLQKTQ 123

Query: 62  RYNDIMQKVEGALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDKY 121
           RYNDIMQKV  AL+ DSN+SNQG VLEDDPEYQLIVECN+LSVDIENEIIIIHNFIRDKY
Sbjct: 124 RYNDIMQKVADALEMDSNVSNQGFVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDKY 183

Query: 122 RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP 181
           RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP
Sbjct: 184 RLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLP 243

Query: 182 EEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLAA 241
           EEILQKTIDACDRAL LDSAKK VLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGL A
Sbjct: 244 EEILQKTIDACDRALTLDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLVA 303

Query: 242 LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKST 301
           LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKST
Sbjct: 304 LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKST 363

Query: 302 LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR 361
           LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR
Sbjct: 364 LAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLR 423

Query: 362 KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV 421
           KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV
Sbjct: 424 KMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKV 483

Query: 422 VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFSKI 481
           VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLG GTQSTYFSETGTFSKI
Sbjct: 484 VKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGGGTQSTYFSETGTFSKI 543

Query: 482 RKN 484
           RKN
Sbjct: 544 RKN 546

BLAST of MC09g1753 vs. ExPASy TrEMBL
Match: A0A6J1DBC9 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 OS=Momordica charantia OX=3673 GN=LOC111019139 PE=3 SV=1)

HSP 1 Score: 905 bits (2339), Expect = 0.0
Identity = 484/484 (100.00%), Postives = 484/484 (100.00%), Query Frame = 0

Query: 1   MATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKT 60
           MATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKT
Sbjct: 1   MATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKT 60

Query: 61  QRYNDIMQKVEGALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDK 120
           QRYNDIMQKVEGALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDK
Sbjct: 61  QRYNDIMQKVEGALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL
Sbjct: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180

Query: 181 PEEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240
           PEEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA
Sbjct: 181 PEEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240

Query: 241 ALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKS 300
           ALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKS
Sbjct: 241 ALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL
Sbjct: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420
           RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK
Sbjct: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420

Query: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFSK 480
           VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFSK
Sbjct: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFSK 480

Query: 481 IRKN 484
           IRKN
Sbjct: 481 IRKN 484

BLAST of MC09g1753 vs. ExPASy TrEMBL
Match: A0A6J1G0W2 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog OS=Cucurbita moschata OX=3662 GN=LOC111449657 PE=3 SV=1)

HSP 1 Score: 886 bits (2289), Expect = 0.0
Identity = 472/484 (97.52%), Postives = 479/484 (98.97%), Query Frame = 0

Query: 1   MATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKT 60
           MATLADSFLADLDELSDEDNF DEEDVDAENMEEDIDGDLADLESLNYDDLD+VSKLQK+
Sbjct: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60

Query: 61  QRYNDIMQKVEGALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDK 120
           QRY+DIMQKVE ALQKDSNISNQGLVLEDDPEYQLIVECN+LSVDIENEIIIIHNFIRDK
Sbjct: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL
Sbjct: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180

Query: 181 PEEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240
           PEEILQKTIDACDRALALDSAKK VLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGL 
Sbjct: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLG 240

Query: 241 ALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKS 300
           +LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRL++AKS
Sbjct: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLVSAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL
Sbjct: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420
           RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK
Sbjct: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420

Query: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFSK 480
           VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLG GTQSTYFSETGTFSK
Sbjct: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGGGTQSTYFSETGTFSK 480

Query: 481 IRKN 484
           IRKN
Sbjct: 481 IRKN 484

BLAST of MC09g1753 vs. ExPASy TrEMBL
Match: A0A6J1KRI6 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111497587 PE=3 SV=1)

HSP 1 Score: 885 bits (2287), Expect = 0.0
Identity = 472/484 (97.52%), Postives = 475/484 (98.14%), Query Frame = 0

Query: 1   MATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKT 60
           MATLADSFLADLDELSDEDNFLDEED DAENMEEDIDGDLADLESLNYDDLDSVSKLQKT
Sbjct: 1   MATLADSFLADLDELSDEDNFLDEEDADAENMEEDIDGDLADLESLNYDDLDSVSKLQKT 60

Query: 61  QRYNDIMQKVEGALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDK 120
           QRYNDIMQKV  AL+ DSN+SNQG VLEDDPEYQLIVECN+LSVDIENEIIIIHNFIRDK
Sbjct: 61  QRYNDIMQKVADALEMDSNVSNQGFVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL
Sbjct: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180

Query: 181 PEEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240
           PEEILQKTIDACDRAL LDSAKK VLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGL 
Sbjct: 181 PEEILQKTIDACDRALTLDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLV 240

Query: 241 ALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKS 300
           ALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKS
Sbjct: 241 ALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL
Sbjct: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420
           RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK
Sbjct: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420

Query: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFSK 480
           VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLG GTQSTYFSETGTFSK
Sbjct: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGGGTQSTYFSETGTFSK 480

Query: 481 IRKN 484
           IRKN
Sbjct: 481 IRKN 484

BLAST of MC09g1753 vs. ExPASy TrEMBL
Match: A0A6J1GXP7 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458320 PE=3 SV=1)

HSP 1 Score: 885 bits (2287), Expect = 0.0
Identity = 472/484 (97.52%), Postives = 475/484 (98.14%), Query Frame = 0

Query: 1   MATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKT 60
           MATLADSFLADLDELSDEDNFLDEED DAENMEEDIDGDLADLESLNYDDLDSVSKLQKT
Sbjct: 1   MATLADSFLADLDELSDEDNFLDEEDADAENMEEDIDGDLADLESLNYDDLDSVSKLQKT 60

Query: 61  QRYNDIMQKVEGALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDK 120
           QRYNDIMQKV  AL+ DSN+SNQG VLEDDPEYQLIVECN+LSVDIENEIIIIHNFIRDK
Sbjct: 61  QRYNDIMQKVADALEMDSNVSNQGFVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL
Sbjct: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180

Query: 181 PEEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240
           PEEILQKTIDACDRAL LDSAKK VLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGL 
Sbjct: 181 PEEILQKTIDACDRALTLDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLV 240

Query: 241 ALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKS 300
           ALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKS
Sbjct: 241 ALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL
Sbjct: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420
           RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK
Sbjct: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420

Query: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFSK 480
           VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLG GTQSTYFSETGTFSK
Sbjct: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGGGTQSTYFSETGTFSK 480

Query: 481 IRKN 484
           IRKN
Sbjct: 481 IRKN 484

BLAST of MC09g1753 vs. ExPASy TrEMBL
Match: A0A6J1HSU1 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog OS=Cucurbita maxima OX=3661 GN=LOC111466452 PE=3 SV=1)

HSP 1 Score: 884 bits (2283), Expect = 0.0
Identity = 471/484 (97.31%), Postives = 480/484 (99.17%), Query Frame = 0

Query: 1   MATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKT 60
           MATLADSFLADLDELSDEDNF DEEDVDAENMEEDIDGDLADLESLNYDDLD+VSKLQK+
Sbjct: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60

Query: 61  QRYNDIMQKVEGALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDK 120
           QRY+DIMQKVE ALQKDSNISNQGLVLEDDPEYQLIVECN+LSVDIENEIIIIHNFIRDK
Sbjct: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL
Sbjct: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180

Query: 181 PEEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240
           PEEILQKTIDACDRALALDSAKK VLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA
Sbjct: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240

Query: 241 ALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKS 300
           +LAKMPACNVQLLGAKKKNLAGFSTATSQFR+GYIEQTEIFQSTPP LKMRACRL++AKS
Sbjct: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRLGYIEQTEIFQSTPPHLKMRACRLVSAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL
Sbjct: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420
           RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK
Sbjct: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420

Query: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFSK 480
           VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAH+NQLGSGTQSTYFSETGTFSK
Sbjct: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHMNQLGSGTQSTYFSETGTFSK 480

Query: 481 IRKN 484
           IRKN
Sbjct: 481 IRKN 484

BLAST of MC09g1753 vs. TAIR 10
Match: AT1G60170.1 (pre-mRNA processing ribonucleoprotein binding region-containing protein )

HSP 1 Score: 697.2 bits (1798), Expect = 9.3e-201
Identity = 376/485 (77.53%), Postives = 421/485 (86.80%), Query Frame = 0

Query: 1   MATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKT 60
           MATL DSFLADLDELSD +  LDE D D    EED+D D+ADLE+LNYDDLD+VSKLQK+
Sbjct: 1   MATLEDSFLADLDELSDNEAELDENDGDVGKEEEDVDMDMADLETLNYDDLDNVSKLQKS 60

Query: 61  QRYNDIMQKVEGALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDK 120
           QRY DIM KVE AL KDS+ + +G VLEDDPEY+LIV+CN LSVDIENEI+I+HNFI+DK
Sbjct: 61  QRYADIMHKVEEALGKDSDGAEKGTVLEDDPEYKLIVDCNQLSVDIENEIVIVHNFIKDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           Y+LKF ELESLVHHPIDYA VVKKIGNE DL LVDL  LLPSA+IMVVSVTA TT G  L
Sbjct: 121 YKLKFQELESLVHHPIDYACVVKKIGNETDLALVDLADLLPSAIIMVVSVTALTTKGSAL 180

Query: 181 PEEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240
           PE++LQK ++ACDRAL LDSA+KKVL FVES+MG IAPNLSAIVGSAVAAKLMGTAGGL+
Sbjct: 181 PEDVLQKVLEACDRALDLDSARKKVLEFVESKMGSIAPNLSAIVGSAVAAKLMGTAGGLS 240

Query: 241 ALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKS 300
           ALAKMPACNVQ+LG K+KNLAGFS+ATSQ RVGY+EQTEI+QSTPP L+ RA RL+AAKS
Sbjct: 241 ALAKMPACNVQVLGHKRKNLAGFSSATSQSRVGYLEQTEIYQSTPPGLQARAGRLVAAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVD+TRGDP G +G+ F++EI KKIEKWQEPPPA+QPKPLPVPDSEPKK+RGGRRL
Sbjct: 301 TLAARVDATRGDPLGISGKAFREEIRKKIEKWQEPPPARQPKPLPVPDSEPKKRRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLA-- 420
           RKMKERY  T+MRKLANRM FG PEESSLGDGLGEGYGMLGQAGS +LRVS+  SKL   
Sbjct: 361 RKMKERYQVTDMRKLANRMAFGTPEESSLGDGLGEGYGMLGQAGSNRLRVSSVPSKLKIN 420

Query: 421 AKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTF 480
           AKV KK KE++Y     TSGLTSSLAFTPVQGIEL NPQ  L  LGSGTQSTYFSE+GTF
Sbjct: 421 AKVAKKLKERQYAGGATTSGLTSSLAFTPVQGIELCNPQQALG-LGSGTQSTYFSESGTF 480

Query: 481 SKIRK 484
           SK++K
Sbjct: 481 SKLKK 484

BLAST of MC09g1753 vs. TAIR 10
Match: AT1G70400.1 (CONTAINS InterPro DOMAIN/s: NOSIC (InterPro:IPR012976); BEST Arabidopsis thaliana protein match is: pre-mRNA processing ribonucleoprotein binding region-containing protein (TAIR:AT1G60170.1); Has 479 Blast hits to 479 proteins in 178 species: Archae - 0; Bacteria - 0; Metazoa - 125; Fungi - 138; Plants - 124; Viruses - 0; Other Eukaryotes - 92 (source: NCBI BLink). )

HSP 1 Score: 213.0 bits (541), Expect = 5.3e-55
Identity = 118/172 (68.60%), Postives = 137/172 (79.65%), Query Frame = 0

Query: 39  DLADLESLNYDDLDSVSKLQKTQRYNDIMQKVEGALQKDSNISNQGLVLEDDPEYQLIVE 98
           D+ +L +L YDDLDSVSKLQK++RY DIMQ+VE AL        +G VLE     +LIV+
Sbjct: 2   DMTELNTLTYDDLDSVSKLQKSRRYADIMQQVEEAL--------EGSVLE---YKKLIVD 61

Query: 99  CNSLSVDIENEIIIIHNFIRDKYRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEG 158
           C  L VDIENEI+I+ NFIRDKYR+KF ELE LV HPIDYARVVK+IGNEMDL LVDLEG
Sbjct: 62  CKQLLVDIENEIVIVQNFIRDKYRVKFQELELLVPHPIDYARVVKRIGNEMDLKLVDLEG 121

Query: 159 LLPSAVIMVVSVTASTTSGKPLPEEILQKTIDACDRALALDSAKKKVLNFVE 211
           LLPSA+IMV+ VTA TT G  LPE++L KTIDACDRAL LDSA+KKVL FV+
Sbjct: 122 LLPSAMIMVLLVTALTTKGNQLPEDVLLKTIDACDRALDLDSARKKVLEFVD 162

BLAST of MC09g1753 vs. TAIR 10
Match: AT1G70400.2 (CONTAINS InterPro DOMAIN/s: NOSIC (InterPro:IPR012976); BEST Arabidopsis thaliana protein match is: pre-mRNA processing ribonucleoprotein binding region-containing protein (TAIR:AT1G60170.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 205.3 bits (521), Expect = 1.1e-52
Identity = 114/166 (68.67%), Postives = 132/166 (79.52%), Query Frame = 0

Query: 39  DLADLESLNYDDLDSVSKLQKTQRYNDIMQKVEGALQKDSNISNQGLVLEDDPEYQLIVE 98
           D+ +L +L YDDLDSVSKLQK++RY DIMQ+VE AL        +G VLE     +LIV+
Sbjct: 2   DMTELNTLTYDDLDSVSKLQKSRRYADIMQQVEEAL--------EGSVLE---YKKLIVD 61

Query: 99  CNSLSVDIENEIIIIHNFIRDKYRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEG 158
           C  L VDIENEI+I+ NFIRDKYR+KF ELE LV HPIDYARVVK+IGNEMDL LVDLEG
Sbjct: 62  CKQLLVDIENEIVIVQNFIRDKYRVKFQELELLVPHPIDYARVVKRIGNEMDLKLVDLEG 121

Query: 159 LLPSAVIMVVSVTASTTSGKPLPEEILQKTIDACDRALALDSAKKK 205
           LLPSA+IMV+ VTA TT G  LPE++L KTIDACDRAL LDSA+KK
Sbjct: 122 LLPSAMIMVLLVTALTTKGNQLPEDVLLKTIDACDRALDLDSARKK 156

BLAST of MC09g1753 vs. TAIR 10
Match: AT1G70400.3 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: egg cell; CONTAINS InterPro DOMAIN/s: NOSIC (InterPro:IPR012976); BEST Arabidopsis thaliana protein match is: pre-mRNA processing ribonucleoprotein binding region-containing protein (TAIR:AT1G60170.1); Has 484 Blast hits to 484 proteins in 190 species: Archae - 0; Bacteria - 0; Metazoa - 149; Fungi - 134; Plants - 124; Viruses - 0; Other Eukaryotes - 77 (source: NCBI BLink). )

HSP 1 Score: 205.3 bits (521), Expect = 1.1e-52
Identity = 114/166 (68.67%), Postives = 132/166 (79.52%), Query Frame = 0

Query: 39  DLADLESLNYDDLDSVSKLQKTQRYNDIMQKVEGALQKDSNISNQGLVLEDDPEYQLIVE 98
           D+ +L +L YDDLDSVSKLQK++RY DIMQ+VE AL        +G VLE     +LIV+
Sbjct: 2   DMTELNTLTYDDLDSVSKLQKSRRYADIMQQVEEAL--------EGSVLE---YKKLIVD 61

Query: 99  CNSLSVDIENEIIIIHNFIRDKYRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEG 158
           C  L VDIENEI+I+ NFIRDKYR+KF ELE LV HPIDYARVVK+IGNEMDL LVDLEG
Sbjct: 62  CKQLLVDIENEIVIVQNFIRDKYRVKFQELELLVPHPIDYARVVKRIGNEMDLKLVDLEG 121

Query: 159 LLPSAVIMVVSVTASTTSGKPLPEEILQKTIDACDRALALDSAKKK 205
           LLPSA+IMV+ VTA TT G  LPE++L KTIDACDRAL LDSA+KK
Sbjct: 122 LLPSAMIMVLLVTALTTKGNQLPEDVLLKTIDACDRALDLDSARKK 156

BLAST of MC09g1753 vs. TAIR 10
Match: AT3G05060.1 (NOP56-like pre RNA processing ribonucleoprotein )

HSP 1 Score: 101.3 bits (251), Expect = 2.3e-21
Identity = 70/239 (29.29%), Postives = 118/239 (49.37%), Query Frame = 0

Query: 79  NISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDKYRLKFPELESLVHHPIDY 138
           +++   L    D    +I++   L  D++ E+      +R+ Y   FPEL  ++   I Y
Sbjct: 144 SLARYKLKFSSDKVDTMIIQAIGLLDDLDKELNTYAMRVREWYGWHFPELAKIISDNILY 203

Query: 139 ARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLPEEILQKTIDACDRALAL 198
           A+ VK +GN ++   +D   +L   +   +   A  + G  + +  L    + CD+ L+L
Sbjct: 204 AKSVKLMGNRVNAAKLDFSEILADEIEADLKDAAVISMGTEVSDLDLLHIRELCDQVLSL 263

Query: 199 DSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLAALAKMPACNVQLLGAKKK 258
              + ++ ++++SRM  IAPNL+A+VG  V A+L+   G L  L+K P   VQ+LGA+K 
Sbjct: 264 SEYRAQLYDYLKSRMNTIAPNLTALVGELVGARLISHGGSLLNLSKQPGSTVQILGAEKA 323

Query: 259 NLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKSTLAARVDSTRGDPTGKT 318
                 T  +  + G I    +     P  K +  R +AAK+ LA RVD+  GD    T
Sbjct: 324 LFRALKTKHATPKYGLIFHASLVGQAAPKHKGKISRSLAAKTVLAIRVDAL-GDSQDNT 381

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8RXN61.3e-19977.53U4/U6 small nuclear ribonucleoprotein Prp31 homolog OS=Arabidopsis thaliana OX=3... [more]
Q5U5C57.6e-10747.47U4/U6 small nuclear ribonucleoprotein Prp31 OS=Xenopus laevis OX=8355 GN=prpf31 ... [more]
Q8CCF06.5e-10647.18U4/U6 small nuclear ribonucleoprotein Prp31 OS=Mus musculus OX=10090 GN=Prpf31 P... [more]
Q6NVP61.9e-10546.87U4/U6 small nuclear ribonucleoprotein Prp31 OS=Xenopus tropicalis OX=8364 GN=prp... [more]
Q8WWY39.3e-10546.98U4/U6 small nuclear ribonucleoprotein Prp31 OS=Homo sapiens OX=9606 GN=PRPF31 PE... [more]
Match NameE-valueIdentityDescription
XP_022151138.10.0100.00U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 [Momordica charan... [more]
XP_022945419.10.097.52U4/U6 small nuclear ribonucleoprotein Prp31 homolog [Cucurbita moschata] >XP_022... [more]
XP_022956658.10.097.52U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 [Cucurbita moscha... [more]
XP_022966885.10.097.31U4/U6 small nuclear ribonucleoprotein Prp31 homolog [Cucurbita maxima][more]
KAG7032269.10.097.52U4/U6 small nuclear ribonucleoprotein Prp31-like protein, partial [Cucurbita arg... [more]
Match NameE-valueIdentityDescription
A0A6J1DBC90.0100.00U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 OS=Momordica char... [more]
A0A6J1G0W20.097.52U4/U6 small nuclear ribonucleoprotein Prp31 homolog OS=Cucurbita moschata OX=366... [more]
A0A6J1KRI60.097.52U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 OS=Cucurbita maxi... [more]
A0A6J1GXP70.097.52U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 OS=Cucurbita mosc... [more]
A0A6J1HSU10.097.31U4/U6 small nuclear ribonucleoprotein Prp31 homolog OS=Cucurbita maxima OX=3661 ... [more]
Match NameE-valueIdentityDescription
AT1G60170.19.3e-20177.53pre-mRNA processing ribonucleoprotein binding region-containing protein [more]
AT1G70400.15.3e-5568.60CONTAINS InterPro DOMAIN/s: NOSIC (InterPro:IPR012976); BEST Arabidopsis thalian... [more]
AT1G70400.21.1e-5268.67CONTAINS InterPro DOMAIN/s: NOSIC (InterPro:IPR012976); BEST Arabidopsis thalian... [more]
AT1G70400.31.1e-5268.67FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
AT3G05060.12.3e-2129.29NOP56-like pre RNA processing ribonucleoprotein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012976NOSICSMARTSM00931NOSIC_2coord: 95..147
e-value: 4.8E-23
score: 92.6
IPR019175Prp31 C-terminalPFAMPF09785Prp31_Ccoord: 339..456
e-value: 3.8E-43
score: 147.3
NoneNo IPR availableGENE3D1.10.287.4070coord: 78..216
e-value: 3.6E-57
score: 194.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 330..361
NoneNo IPR availablePANTHERPTHR13904:SF0U4/U6 SMALL NUCLEAR RIBONUCLEOPROTEIN PRP31coord: 1..481
IPR002687Nop domainPFAMPF01798Nopcoord: 103..331
e-value: 1.1E-73
score: 247.5
IPR002687Nop domainPROSITEPS51358NOPcoord: 216..334
score: 37.543999
IPR042239Nop, C-terminal domainGENE3D1.10.246.90Nop domaincoord: 217..342
e-value: 1.1E-40
score: 140.3
IPR027105U4/U6 small nuclear ribonucleoprotein Prp31PANTHERPTHR13904PRE-MRNA SPLICING FACTOR PRP31coord: 1..481
IPR036070Nop domain superfamilySUPERFAMILY89124Nop domaincoord: 88..334

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g1753.1MC09g1753.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000244 spliceosomal tri-snRNP complex assembly
biological_process GO:0000398 mRNA splicing, via spliceosome
cellular_component GO:0071011 precatalytic spliceosome
cellular_component GO:0005687 U4 snRNP
cellular_component GO:0046540 U4/U6 x U5 tri-snRNP complex
molecular_function GO:0003723 RNA binding