MC09g1055 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g1055
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionphosphoglucan phosphatase LSF2, chloroplastic
LocationMC09: 16441404 .. 16446900 (+)
RNA-Seq ExpressionMC09g1055
SyntenyMC09g1055
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTAGTCGGAGATATATGCCAATTACTATTGAGTTATTAGTGATATTTTTTTGTAATACGATGTAAATGCCGATTTAGGTAGGTGGTAAGTTTGAATTCATTCTCTCTTATTAATTCACCTTAATCCATTCAGACCAATGAGCCATCCAATATTTTTGCAATCTATAAAGATTTAATTAAATGTACAACACCTTTGTAACTCTGTGGCAGGTTAAGAGTGATCAAATTAAAAATTAGAGCCTGTAAATATATATTTTTTTTCTTTTATGAGTTGAACAATGGGATGAACTCAACTTTACTCGGTATTGCATCGACATCCCTTGTTAGGTAGTTAGCCAAATTTTGCCATGGATAGCTGGAATCGTCTCCTGGAAGTAATTTTCACGTGCAAGGTCGGTGGATTTGCTTCTAAAAGCTAAACCCCCAAATTCAACCCACCCAATTTCATCGAAAATGGCAACTTCAAGTTTTCCCCCTTCTTTTTCATCCCTCTCCGTAATTCTTCCCCTTCAGAATCATACACCCAACAAATTTTTATCATTCCCTTCGAAGCTTTCGAAACCCCACTTGCAATTCCACAGAATCTGCTGCGGAATGCCTGAGACTGGGGTTCGGCAAAACCCCACAAGCGGCGGAGCTTCCACTTCCAGGAACAGCACGGAGATGTACAATATGACTATGAAGAAGATGATGAAGAACCCTTATGAGTATCACCATGAACTGGGTCAGTTTCTTCTTTTCTTCATCCTGTTTTTCGATTTTTCCTTTTGATCCTTGAATTCTGTAATTCACGGCCAGCCATTTTATATGCTTCGATCGCTTTCCTTCTGCAATTTGTTAATGGGTGATTTTGGAGCATTTGTGTTGTTTGGAATGAGAAGTAATTGGGCGATAGGATTGATATTGGGACTTTAGAAGTTCATGAATAGAAGTTTTTTTTTTTTTTTTTTTTTCTGTTTCCTAGATATTGAGCCTTCTCTGTGGATTTCTAATGATTTATGATAGCTTTTCGCAAAGTTACCCAATGTTAATGGTGCTTTGAGTAATTAGAGGGCAAAGACATTTTGTAACCTCTTTGAGTTCTTTGAGAAGTCCATAAAATTGTGGGCTCTCTCTTTGAGTTTCAGTTTACTAGATTTGATTTTAGGAGCAGTTTTACTGGTTACTTAGGTTCTGTTTGGTTCATGATTCAAATAGAAGGGATTAAATAGATAAGAGAGAAGAAGCTATTAGAGAGAGAGACTCTGCTTTTGAGAAAATACAGGGTTCTACTTTTCAGGAAATCTCTTTCTGTATTCATTTGAAAACAAAGAGAACATTGAAAGAATGATTTCAAAAGTTGGCTTAAATGGGTAATTGTCTTTGAAAATCATTTTCGAAAACACAAGTACCAAATGCAATTCAGTGTATCCTTGGCATCCCTTGTTTTCAAACACGTAAAAGGATAGCGGGTTTGATACCAAATCGGTATGAAGTAACTTTTCCAGTTAGAATTCTGATAGACAAAACTTGAAGTAATATCAGTTTGCTCATAATGCTCCTGCAATCAGGTATGAACTATACTGTGATAGCTGACAATTTGATTGTCGGTTCCCAGCCTCAAAAACCCAAAGATATCGATTTCTTGAAGGAGGAAGAAGGTGTTGCCTATATTCTGAATTTACAGCAGGACAAGGATGTTGAGTATTGGGGAGTCGACTTACGATCAATTATAGAAAGATGTAAAGAACTTGGAATTCATCATATGAGGAAACCGGTAAGCACATATACTTTTCATACCCTGTGTCTTAAATCTCTGGTAGACCCATTTTCAAATATATACTGATTTCTCTCCCTTTTTAATCTTGAACTGCTGGAAACTGATTTCTAACTGTTCTATGTTGTATGTACTTTCATCAAATGGAAATATGAAATCTGGGTATGGGAAAAGCTTTTTCTTAAGCAGGAAATGTTCTTCACTCAAGTTTATTTTCAGCTTATCTGTGCATTAAGAAAGTAAATTAAAAAAAGGAAAGCATGGTTTCTCATAAAGATAATAATAATAAATAAAAGTTTATATACTATTTTGGTTGCTATACTTTTAGCTCTTGTTTATTTTGATCCATGTAATTTCAAAACATAGATCTTGGTCCATGTACTTTCATATTTTATTATTTTATCCATCTTAGTCCTTATACTTTCTGAATGTCTACTTTTAAAAAATGATTGTTTTGGTCCATTCATTTTCCTTTTTGTCTCATTTTTTTCCTATCACAATTTCAACGTGACGATATACTCAATAAATTTCTTGCAATGTACTCATAACTTTGTATTAAAGGGTTTTAATTTGTGTTAAAATGTTGCAAACAAAGACCAAAGTAGTCACTTTTTTTCTCACAAAAAAAAGTAGCGACATTTTAAAAGTACAAGGCTAAATTGGATGTTTTGAGTGTAAAAAACTAAAATGAACCAAAGTTGAACGTATAGGACCCAAATGAACATTTTTAAAGTATAAGGACTTGAATGAACTGAGCATGAATTTCCAGGGACTAAAATAGTATTTAAACTACAAATAAATAAATAATTAATTAAATAAATTTAGCGTAACTCATTTGCTTAAGTTTTTGGGTTCAAGGGTGATTTAACATATTATTATAGTAGTCTAGTATGAGGTCCTTTATTCGAATTTCAATAATATAATTTTCTTCTTATTTAATATTGATAATAACTCGTGGAGTATTTTAGAGTTTGAAATTAGCTTAAGCTTTTGGATTCAACGATTATTTAGCACTACGAAACAAACGAAACAAGTGAGAGACTAGTATCTCTTGTAGCAACTTGCACTAACGTCGTGAATTTGTCTGCTCTGTTTGTTTTTGGTGGCACGTCGGGTTAACCCATTCTTTTGTAGGCAAGAGATTTTGATCCAGATTCCATGAGAAATGGATTACCTAAAGCTGTTTCCTTGCTAGAATGGGCGATCTCGGAGGGTAAAGGAAAAGTTTATGTCCATTGCACCGCTGGACTAGGAAGGGCTCCTGCCGTTGCAATAGCTTACTTGTTCTGGTTTTGTGGAATGAATGTGAGTTCTTCTTTTACTTAGAAATTAATGCAGTACATTAATAATGCCTGCTCTTGACATGTTTTGAAGCTGGAAAGTGTAAAATATGACAGTAGATATCAATGTTGACATTTTCTGATTTACACAGACTTTTTTACTCCTCAAAATAAAACTACCAAGTTATTGGTTTCTTATTTTGGCTATGTGACAGTTAAATACCGCATATGAAGCACTGACCTCGAAGCGACCTTGTGGACCGAGTAAAAGAGCGATTCGTGGAGCCACATACGATCTATGTAAAACAGATCAATGGAAGGAGCCATTCGAGAGCCTTCCCGACAATGCATTTGAAGGTGTTGCAGATTGGGAGAGAAACTTGATTCAGGATCGGGTCCGCTCTCTCCGCAAAACTTGAGTAATTGTGCATCGTTTTCCCTTTGCTTACTTGGTCCTGAAACTTCCTAGTTTGAGTTGAAATTTTATAAGGTCTTCTAGGGGCAATTTGGTTTAAGGTTTCGAGAAATATCCATCGGTCAAGGAATAAGATGTTTGGGAATCAAAGATAACTGTTTTTGGTTAGGTAAGGAAATACCACCAGATTTTCCTAAAAAATAATTATAGGTGTTTATTATAATAATTATTATTATTTAAATATCCGAGTTTATTTTGTGTTATTGCTCTAGAAAATATGATTTTTCTAAAAAATAATTATAGGTGTTTATTATAATTATTATTATTATTTAAATATCCGAGTTTATTTTGTGTTATTGCTCTAGAAAATATGAATTTTATGTCACCATAGTGATGGATAACTAATCAAATTTAATTCAAATAAATAATATAAATAAAATTATTTAAAGTTATTAGTTATTTTATATTGACATTCTAAAATATTATTTTATGTTAGTTATGTCTAATTATTGAATTATTTCTATATAATTCAATTTGATTCTATTCAACTCAACCTAATTGATTCCTACTTTTCATTTAATTTTTATTAATTAGTAAAAATTAATTTACATCAATTAAGTTTAATTAGTAAATTGATTGTAGAGTGATTTAAGTTATTTTAGTTAAATAATTTAAAATCAATTTAGTAAATTGATTTTTTTAGCAATCCATTTTAATGGATTAATTTATATCAAATATATTTAATTAATTAAGTATAAATCATATAAATTTGAAATTTTAGTTATATTGAGCAATTAAGCACCACAATTAATAGTAAATGTTTTTCATTTTTTAGTATGACGTGGGGTGAAAAGTCAAGCTTATGACTTTTTGGTCGTAGATAGCTACTTTAATCTGATGAGTTATATTCAAGCCAACATTAATAAATATTAGTTAATTAAAATTCAACCTATTTTATAATTAATTAGTTCAAATTTTATTATTAAAAAAACTCGTTTAGAGTTTTATTGGAAGGTTAAAATATATATATTAAAATATTTGTAGTTTTCAAATTGGTTAAAAAACACATTTGGTCTCAGTACTTTCATTAAAGTAACGATTTAGTCTCTGAACTTTTAATTGTAACGATTTAGTTCCTACAGTTAAAAATTTGTAACAATTTACTCCCTACTATGAAAATTCTCATCAAAATTAAGCGTCAATTTTTATCATATTATGATTTAATTTTTGTAATTTATAAACTAATTCGATCCTTAATCGGTTTATCAATCTAAATATATAAAAAATTTAACCAAAAAAATTTACAATGGAGATTAAATTATTACAAATTTTAAACTTTAGAAATTAAATTGTTATATTTTAAATTTTATGGACTAAATTGTTACCAATTTGAAAGTATAGGGATTAAATCATTATTTTTTAAAGTTTATGAACTAAATCATTACTTCAACTAAAATACGAGGACCAAAAGTGTTATTTAACCTATTTAAATCTATTAAACTAGTATAAGAAAAAATATATATAATAAATTAAACTTTTGGACATTTGACAAACTCACGACAATAGAATGGTAAAGAAAATCCTTGATATCTTGAAATAAGTTTTTATCAAGTAAAAGTGTTCAAAATTATGTATCAAATTTGGGTTTAACTTGCCTATTGTCGCCTAACAAAATTTCTAATCAAATGGTTTAAACTTTCTTTTTATTTGTTGCAGCTCTATTTGGACTATCATGATTTCGACCTGGAATTGGTCGAGGAAATCAAAATAGGTAGTTGTTCGTGGTAAAACATGAAGGTTCGATAATGTTCGGTATTACTAGGATGATGGATTGGACAACAAATGTAGCAATAAGGTGACACAATTTGCTATATTGCATAAATGTCAATCTGTATAGAACACTAAAATGATTCAAAGTAATATATAGTGGTGTTAATGGTACTTTTGGATCAAACCTAATCGTGAATTGTATATTTATTCAATCCAAACCATCTTTATTAAATATTAAATTCAACTCAATCCAATCATGAAATATTTAGGTTGAGT

mRNA sequence

CTTAGTCGGAGATATATGCCAATTACTATTGAGTTATTAGTGATATTTTTTTGTAATACGATGTAAATGCCGATTTAGGTAGGTGGTAAGTTTGAATTCATTCTCTCTTATTAATTCACCTTAATCCATTCAGACCAATGAGCCATCCAATATTTTTGCAATCTATAAAGATTTAATTAAATGTACAACACCTTTGTAACTCTGTGGCAGGTTAAGAGTGATCAAATTAAAAATTAGAGCCTGTAAATATATATTTTTTTTCTTTTATGAGTTGAACAATGGGATGAACTCAACTTTACTCGGTATTGCATCGACATCCCTTGTTAGGTAGTTAGCCAAATTTTGCCATGGATAGCTGGAATCGTCTCCTGGAAGTAATTTTCACGTGCAAGGTCGGTGGATTTGCTTCTAAAAGCTAAACCCCCAAATTCAACCCACCCAATTTCATCGAAAATGGCAACTTCAAGTTTTCCCCCTTCTTTTTCATCCCTCTCCGTAATTCTTCCCCTTCAGAATCATACACCCAACAAATTTTTATCATTCCCTTCGAAGCTTTCGAAACCCCACTTGCAATTCCACAGAATCTGCTGCGGAATGCCTGAGACTGGGGTTCGGCAAAACCCCACAAGCGGCGGAGCTTCCACTTCCAGGAACAGCACGGAGATGTACAATATGACTATGAAGAAGATGATGAAGAACCCTTATGAGTATCACCATGAACTGGGTATGAACTATACTGTGATAGCTGACAATTTGATTGTCGGTTCCCAGCCTCAAAAACCCAAAGATATCGATTTCTTGAAGGAGGAAGAAGGTGTTGCCTATATTCTGAATTTACAGCAGGACAAGGATGTTGAGTATTGGGGAGTCGACTTACGATCAATTATAGAAAGATGTAAAGAACTTGGAATTCATCATATGAGGAAACCGGCAAGAGATTTTGATCCAGATTCCATGAGAAATGGATTACCTAAAGCTGTTTCCTTGCTAGAATGGGCGATCTCGGAGGGTAAAGGAAAAGTTTATGTCCATTGCACCGCTGGACTAGGAAGGGCTCCTGCCGTTGCAATAGCTTACTTGTTCTGGTTTTGTGGAATGAATTTAAATACCGCATATGAAGCACTGACCTCGAAGCGACCTTGTGGACCGAGTAAAAGAGCGATTCGTGGAGCCACATACGATCTATGTAAAACAGATCAATGGAAGGAGCCATTCGAGAGCCTTCCCGACAATGCATTTGAAGGTGTTGCAGATTGGGAGAGAAACTTGATTCAGGATCGGCTCTATTTGGACTATCATGATTTCGACCTGGAATTGGTCGAGGAAATCAAAATAGGTAGTTGTTCGTGGTAAAACATGAAGGTTCGATAATGTTCGGTATTACTAGGATGATGGATTGGACAACAAATGTAGCAATAAGGTGACACAATTTGCTATATTGCATAAATGTCAATCTGTATAGAACACTAAAATGATTCAAAGTAATATATAGTGGTGTTAATGGTACTTTTGGATCAAACCTAATCGTGAATTGTATATTTATTCAATCCAAACCATCTTTATTAAATATTAAATTCAACTCAATCCAATCATGAAATATTTAGGTTGAGT

Coding sequence (CDS)

ATGGCAACTTCAAGTTTTCCCCCTTCTTTTTCATCCCTCTCCGTAATTCTTCCCCTTCAGAATCATACACCCAACAAATTTTTATCATTCCCTTCGAAGCTTTCGAAACCCCACTTGCAATTCCACAGAATCTGCTGCGGAATGCCTGAGACTGGGGTTCGGCAAAACCCCACAAGCGGCGGAGCTTCCACTTCCAGGAACAGCACGGAGATGTACAATATGACTATGAAGAAGATGATGAAGAACCCTTATGAGTATCACCATGAACTGGGTATGAACTATACTGTGATAGCTGACAATTTGATTGTCGGTTCCCAGCCTCAAAAACCCAAAGATATCGATTTCTTGAAGGAGGAAGAAGGTGTTGCCTATATTCTGAATTTACAGCAGGACAAGGATGTTGAGTATTGGGGAGTCGACTTACGATCAATTATAGAAAGATGTAAAGAACTTGGAATTCATCATATGAGGAAACCGGCAAGAGATTTTGATCCAGATTCCATGAGAAATGGATTACCTAAAGCTGTTTCCTTGCTAGAATGGGCGATCTCGGAGGGTAAAGGAAAAGTTTATGTCCATTGCACCGCTGGACTAGGAAGGGCTCCTGCCGTTGCAATAGCTTACTTGTTCTGGTTTTGTGGAATGAATTTAAATACCGCATATGAAGCACTGACCTCGAAGCGACCTTGTGGACCGAGTAAAAGAGCGATTCGTGGAGCCACATACGATCTATGTAAAACAGATCAATGGAAGGAGCCATTCGAGAGCCTTCCCGACAATGCATTTGAAGGTGTTGCAGATTGGGAGAGAAACTTGATTCAGGATCGGCTCTATTTGGACTATCATGATTTCGACCTGGAATTGGTCGAGGAAATCAAAATAGGTAGTTGTTCGTGGTAA

Protein sequence

MATSSFPPSFSSLSVILPLQNHTPNKFLSFPSKLSKPHLQFHRICCGMPETGVRQNPTSGGASTSRNSTEMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKPKDIDFLKEEEGVAYILNLQQDKDVEYWGVDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLEWAISEGKGKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLCKTDQWKEPFESLPDNAFEGVADWERNLIQDRLYLDYHDFDLELVEEIKIGSCSW
Homology
BLAST of MC09g1055 vs. ExPASy Swiss-Prot
Match: Q9SRK5 (Phosphoglucan phosphatase LSF2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LSF2 PE=1 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 1.5e-100
Identity = 172/259 (66.41%), Postives = 209/259 (80.69%), Query Frame = 0

Query: 20  QNHTPNKFLSFPSKLSKPHLQFHRICCGMPETGVRQNPTSGGAS-TSRNSTEMYNMTMKK 79
           +N   + F S  +K S   L+F R   G+      +NP + G S +S+N  E YN  MK+
Sbjct: 19  ENEKSSCFTSI-NKKSSLDLRFPRNLAGVSCKFSGENPGTNGVSLSSKNKMEDYNTAMKR 78

Query: 80  MMKNPYEYHHELGMNYTVIADNLIVGSQPQKPKDIDFLKEEEGVAYILNLQQDKDVEYWG 139
           +M++PYEYHH+LGMNYT+I D LIVGSQPQKP+DID LK+E+ VAYILNLQQDKD+EYWG
Sbjct: 79  LMRSPYEYHHDLGMNYTLIRDELIVGSQPQKPEDIDHLKQEQNVAYILNLQQDKDIEYWG 138

Query: 140 VDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLEWAISEGKGKVYVHCTAGL 199
           +DL SI+ RCKELGI HMR+PA+DFDP S+R+ LPKAVS LEWA+SEGKG+VYVHC+AGL
Sbjct: 139 IDLDSIVRRCKELGIRHMRRPAKDFDPLSLRSQLPKAVSSLEWAVSEGKGRVYVHCSAGL 198

Query: 200 GRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLCKTDQWKEPFESLP 259
           GRAP V+IAY++WFC MNLNTAY+ L SKRPCGP+K AIRGATYDL K D WKEPFESLP
Sbjct: 199 GRAPGVSIAYMYWFCDMNLNTAYDTLVSKRPCGPNKGAIRGATYDLAKNDPWKEPFESLP 258

Query: 260 DNAFEGVADWERNLIQDRL 278
           +NAFE +ADWER LIQ+R+
Sbjct: 259 ENAFEDIADWERKLIQERV 276

BLAST of MC09g1055 vs. ExPASy Swiss-Prot
Match: Q9FEB5 (Phosphoglucan phosphatase DSP4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DSP4 PE=1 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 3.9e-37
Identity = 90/206 (43.69%), Postives = 115/206 (55.83%), Query Frame = 0

Query: 61  GASTSRNSTEMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKPKDIDFLKEEE 120
           G +     ++ Y+  M + M     Y HELGMNY  I  +LIVGS  Q P+D+D L+ + 
Sbjct: 65  GVAKDEEKSDEYSQDMTQAMGAVLTYRHELGMNYNFIRPDLIVGSCLQTPEDVDKLR-KI 124

Query: 121 GVAYILNLQQDKDVEYWGVDLRSIIERCKELG-IHHMRKPARDFDPDSMRNGLPKAVSLL 180
           GV  I  LQQD D+EY+GVD+ SI    K+   I H+R   RDFD   +R  LP  V  L
Sbjct: 125 GVKTIFCLQQDPDLEYFGVDISSIQAYAKKYSDIQHIRCEIRDFDAFDLRMRLPAVVGTL 184

Query: 181 EWAISEGKGKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRG 240
             A+    G  YVHCTAG+GRAPAVA+ Y+FW  G  L  A++ L SKR C P   AIR 
Sbjct: 185 YKAVKRNGGVTYVHCTAGMGRAPAVALTYMFWVQGYKLMEAHKLLMSKRSCFPKLDAIRN 244

Query: 241 ATYDLCKTDQWKEPFESLPDNAFEGV 266
           AT D+    + K    +L D  F  V
Sbjct: 245 ATIDILTGLKRKTVTLTLKDKGFSRV 269

BLAST of MC09g1055 vs. ExPASy Swiss-Prot
Match: G4LTX4 (Phosphoglucan phosphatase DSP4, amyloplastic OS=Castanea sativa OX=21020 GN=DSP4 PE=1 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 1.5e-36
Identity = 92/224 (41.07%), Postives = 118/224 (52.68%), Query Frame = 0

Query: 38  HLQFHRICCGMPETGVRQNPTSGGASTSRNSTEMYNMTMKKMMKNPYEYHHELGMNYTVI 97
           ++  HR       +G   +  +  A+     +E Y+  M + M     Y HELG+NY  I
Sbjct: 37  NVNLHRSMAVKAISGPTSSAETSDANVEEEKSETYSTNMTEAMGAVLTYRHELGINYNFI 96

Query: 98  ADNLIVGSQPQKPKDIDFLKEEEGVAYILNLQQDKDVEYWGVDLRSIIERCKEL-GIHHM 157
             +LIVGS  Q P+D+D L+   GV  I  LQQ+ D+EY+GVD+  I E  K    I H+
Sbjct: 97  RPDLIVGSCLQTPEDVDKLR-SIGVKTIFCLQQNSDLEYFGVDINGIREYAKTYDDIQHL 156

Query: 158 RKPARDFDPDSMRNGLPKAVSLLEWAISEGKGKVYVHCTAGLGRAPAVAIAYLFWFCGMN 217
           R   RDFD   +R  LP  VS L  AI+   G  YVHCTAGLGRAPAV +AY+FW  G  
Sbjct: 157 RAEIRDFDAFDLRVRLPAVVSKLYKAINRNGGVTYVHCTAGLGRAPAVTLAYMFWVQGYK 216

Query: 218 LNTAYEALTSKRPCGPSKRAIRGATYDLCK-------TDQWKEP 254
           L  A+  L SKR C P   AI+ A  D+         T  WK P
Sbjct: 217 LIEAHNLLQSKRSCFPKLDAIKSAAADILTGLRKKVVTLTWKNP 259

BLAST of MC09g1055 vs. ExPASy Swiss-Prot
Match: F4J117 (Phosphoglucan phosphatase LSF1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LSF1 PE=1 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 1.9e-23
Identity = 59/164 (35.98%), Postives = 88/164 (53.66%), Query Frame = 0

Query: 81  KNPYEYHHELGMNYTVIADNLIVGSQPQKPKDIDFLKEEEGVAYILNLQQDKDVEYWGVD 140
           K    Y+H LGM Y+ I + + VGS  Q  +D++ L  E G+  ILN Q   + + WG+D
Sbjct: 279 KGELSYNHALGMRYSKITEQIYVGSCIQTEEDVENL-SEAGITAILNFQGGTEAQNWGID 338

Query: 141 LRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLEWAISEGKGKVYVHCTAGLGR 200
            +SI + C++  +  +  P +D D   +R  LP  V LL   + +   +V+V CT G  R
Sbjct: 339 SQSINDACQKSEVLMINYPIKDADSFDLRKKLPLCVGLLLRLLKKNH-RVFVTCTTGFDR 398

Query: 201 APAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDL 245
           + A  IAYL W    +L+ AY  +T    C P + AI  AT+DL
Sbjct: 399 SSACVIAYLHWMTDTSLHAAYSFVTGLHACKPDRPAIAWATWDL 440

BLAST of MC09g1055 vs. ExPASy Swiss-Prot
Match: Q1M199 (Laforin OS=Canis lupus familiaris OX=9615 GN=EPM2A PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 2.8e-11
Identity = 49/164 (29.88%), Postives = 80/164 (48.78%), Query Frame = 0

Query: 80  MKNPYEYHHEL----GMNYTVIADNLIVGSQPQKPKDIDF-LKEEEGVAYILNLQQDKDV 139
           MK+  +++  +     M+Y+ I  N+ +GS P++ + I   LK E G+  ++N Q + D+
Sbjct: 139 MKHTTDFYFNIAGHQAMHYSRILPNIWLGSCPRQVEHITIKLKHELGITAVMNFQTEWDI 198

Query: 140 EYWGVDLR---------SIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLEWAISE 199
                            ++I+  KE G+ ++  P  D   +     LP+AV LL  A+ E
Sbjct: 199 VQNSSGCNRYPEPMTPDTMIKLYKEEGLVYIWMPTPDMSTEGRVQMLPQAVCLLH-ALLE 258

Query: 200 GKGKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRP 230
               VYVHC AG+GR+ A    +L +  G NL      L +KRP
Sbjct: 259 NGHTVYVHCNAGVGRSTAAVCGWLQYVMGWNLRKVQYFLMAKRP 301

BLAST of MC09g1055 vs. NCBI nr
Match: XP_022146766.1 (phosphoglucan phosphatase LSF2, chloroplastic [Momordica charantia] >XP_022146767.1 phosphoglucan phosphatase LSF2, chloroplastic [Momordica charantia])

HSP 1 Score: 584 bits (1505), Expect = 2.79e-210
Identity = 276/277 (99.64%), Postives = 277/277 (100.00%), Query Frame = 0

Query: 1   MATSSFPPSFSSLSVILPLQNHTPNKFLSFPSKLSKPHLQFHRICCGMPETGVRQNPTSG 60
           MATSSFPPSFSSLSVILPLQNHTPNKFLSFPSKLSKPHLQFHRICCGMPETGVRQNPTSG
Sbjct: 1   MATSSFPPSFSSLSVILPLQNHTPNKFLSFPSKLSKPHLQFHRICCGMPETGVRQNPTSG 60

Query: 61  GASTSRNSTEMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKPKDIDFLKEEE 120
           GASTSRNSTEMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKPKDIDFLKEEE
Sbjct: 61  GASTSRNSTEMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKPKDIDFLKEEE 120

Query: 121 GVAYILNLQQDKDVEYWGVDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLE 180
           GVAYILNLQQDKDVEYWGVDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLE
Sbjct: 121 GVAYILNLQQDKDVEYWGVDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLE 180

Query: 181 WAISEGKGKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGA 240
           WAISEGKGKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGA
Sbjct: 181 WAISEGKGKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGA 240

Query: 241 TYDLCKTDQWKEPFESLPDNAFEGVADWERNLIQDRL 277
           TYDLCKTDQWKEPFESLPDNAFEGVADWERNLIQDR+
Sbjct: 241 TYDLCKTDQWKEPFESLPDNAFEGVADWERNLIQDRV 277

BLAST of MC09g1055 vs. NCBI nr
Match: XP_011655031.1 (phosphoglucan phosphatase LSF2, chloroplastic [Cucumis sativus] >XP_011655032.1 phosphoglucan phosphatase LSF2, chloroplastic [Cucumis sativus] >KGN50717.1 hypothetical protein Csa_005846 [Cucumis sativus])

HSP 1 Score: 482 bits (1241), Expect = 4.61e-170
Identity = 222/270 (82.22%), Postives = 248/270 (91.85%), Query Frame = 0

Query: 8   PSFSSLSVILPLQNHTPNKFLSFPSKLSKPHLQFHRICCGMPETGVRQNPTSGGASTSRN 67
           P FSSL  + PLQN+ PNKFL F S LSKP  +F+RICCG+PE+G++QNP++   S+S+N
Sbjct: 7   PCFSSLFGVFPLQNNAPNKFLPFSSILSKPLYRFNRICCGVPESGIQQNPSTSRPSSSKN 66

Query: 68  STEMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKPKDIDFLKEEEGVAYILN 127
             E YN+ MKKMM+NPYEYHHELGMNYTVIADNLIVGSQPQKP+DI+FLKEEEGVAYILN
Sbjct: 67  RMEDYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDINFLKEEEGVAYILN 126

Query: 128 LQQDKDVEYWGVDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLEWAISEGK 187
           LQQDKDVEYWG+DL+SIIERCKELGIHHMR+PA+DFDPDS+R GLPKAVSLLEWAIS+GK
Sbjct: 127 LQQDKDVEYWGIDLQSIIERCKELGIHHMRQPAKDFDPDSLRTGLPKAVSLLEWAISKGK 186

Query: 188 GKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLCKT 247
           GKVYVHCTAGLGRAPAVAIAYL+WFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDL K 
Sbjct: 187 GKVYVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLSKN 246

Query: 248 DQWKEPFESLPDNAFEGVADWERNLIQDRL 277
           D WKEPFESLPDNAFE +ADWERNLI+DR+
Sbjct: 247 DPWKEPFESLPDNAFEDIADWERNLIRDRV 276

BLAST of MC09g1055 vs. NCBI nr
Match: XP_038891555.1 (phosphoglucan phosphatase LSF2, chloroplastic [Benincasa hispida])

HSP 1 Score: 482 bits (1240), Expect = 3.10e-169
Identity = 224/278 (80.58%), Postives = 250/278 (89.93%), Query Frame = 0

Query: 1   MATSSFPPSFSSLSVILPLQNHTPNKFLSFPSKLSKPHLQFHRICCGMPETGVRQNPTSG 60
           MA SS P  FS L    P QN+ PNKFL F S +SKPH + +RICCG PE+G++Q P++ 
Sbjct: 44  MAASSLP-CFSLLFAFFPHQNNAPNKFLPFSSIISKPHFRLNRICCGAPESGIQQIPSTS 103

Query: 61  GASTSRNSTEMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKPKDIDFLKEEE 120
            AS+S+N  E YN+ MKKMM+NPYEYHHELGMNYTVIADNLIVGSQPQKP+DI+FLKEEE
Sbjct: 104 RASSSKNRMEEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDINFLKEEE 163

Query: 121 GVAYILNLQQDKDVEYWGVDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLE 180
           GVAYILNLQQDKDVEYWG+DL+SII+RCKELGIHHMR+PA+DFDPDS+RNGLPKAVSLLE
Sbjct: 164 GVAYILNLQQDKDVEYWGIDLQSIIKRCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLE 223

Query: 181 WAISEGKGKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGA 240
           WAISEGKGKVYVHCTAGLGRAPAVAIAYL+WFCGMNLNTAYEALTSKRPCGPSKRAIRGA
Sbjct: 224 WAISEGKGKVYVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKRAIRGA 283

Query: 241 TYDLCKTDQWKEPFESLPDNAFEGVADWERNLIQDRLY 278
           TYDL K D WKEPFESLPDNAFE +ADWERNLI+DR++
Sbjct: 284 TYDLSKNDPWKEPFESLPDNAFEDIADWERNLIRDRVH 320

BLAST of MC09g1055 vs. NCBI nr
Match: XP_008456918.1 (PREDICTED: phosphoglucan phosphatase LSF2, chloroplastic isoform X3 [Cucumis melo] >XP_008456919.1 PREDICTED: phosphoglucan phosphatase LSF2, chloroplastic isoform X3 [Cucumis melo])

HSP 1 Score: 474 bits (1219), Expect = 1.03e-166
Identity = 218/270 (80.74%), Postives = 245/270 (90.74%), Query Frame = 0

Query: 8   PSFSSLSVILPLQNHTPNKFLSFPSKLSKPHLQFHRICCGMPETGVRQNPTSGGASTSRN 67
           P FSSL  + PLQN+ PNK L F S LSKP  +F+RICCG+PE+G++QNP++   S+S+N
Sbjct: 7   PCFSSLFGVFPLQNNAPNKLLPFSSTLSKPLYRFNRICCGVPESGIQQNPSTSRPSSSKN 66

Query: 68  STEMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKPKDIDFLKEEEGVAYILN 127
             + YN+ MKKMM+NPYEYHHELGMNYTVIADNLIVGSQPQKP+DI+FLKEEEGVAYILN
Sbjct: 67  RMDEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDINFLKEEEGVAYILN 126

Query: 128 LQQDKDVEYWGVDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLEWAISEGK 187
           LQQDKDVEYWG++L+SIIERCKELGI HMR+PA+DFDPDS+R GLPKAVSLLEWAIS+GK
Sbjct: 127 LQQDKDVEYWGINLQSIIERCKELGIRHMRQPAKDFDPDSLRTGLPKAVSLLEWAISKGK 186

Query: 188 GKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLCKT 247
           GKVYVHCTAGLGRAPAVAIAYL+WFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDL K 
Sbjct: 187 GKVYVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLSKN 246

Query: 248 DQWKEPFESLPDNAFEGVADWERNLIQDRL 277
           D WKEPFESLPDNAFE +ADWERNLI DR+
Sbjct: 247 DPWKEPFESLPDNAFEDIADWERNLIWDRV 276

BLAST of MC09g1055 vs. NCBI nr
Match: XP_008456916.1 (PREDICTED: phosphoglucan phosphatase LSF2, chloroplastic isoform X2 [Cucumis melo])

HSP 1 Score: 474 bits (1219), Expect = 1.38e-166
Identity = 218/270 (80.74%), Postives = 245/270 (90.74%), Query Frame = 0

Query: 8   PSFSSLSVILPLQNHTPNKFLSFPSKLSKPHLQFHRICCGMPETGVRQNPTSGGASTSRN 67
           P FSSL  + PLQN+ PNK L F S LSKP  +F+RICCG+PE+G++QNP++   S+S+N
Sbjct: 7   PCFSSLFGVFPLQNNAPNKLLPFSSTLSKPLYRFNRICCGVPESGIQQNPSTSRPSSSKN 66

Query: 68  STEMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKPKDIDFLKEEEGVAYILN 127
             + YN+ MKKMM+NPYEYHHELGMNYTVIADNLIVGSQPQKP+DI+FLKEEEGVAYILN
Sbjct: 67  RMDEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDINFLKEEEGVAYILN 126

Query: 128 LQQDKDVEYWGVDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLEWAISEGK 187
           LQQDKDVEYWG++L+SIIERCKELGI HMR+PA+DFDPDS+R GLPKAVSLLEWAIS+GK
Sbjct: 127 LQQDKDVEYWGINLQSIIERCKELGIRHMRQPAKDFDPDSLRTGLPKAVSLLEWAISKGK 186

Query: 188 GKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLCKT 247
           GKVYVHCTAGLGRAPAVAIAYL+WFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDL K 
Sbjct: 187 GKVYVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLSKN 246

Query: 248 DQWKEPFESLPDNAFEGVADWERNLIQDRL 277
           D WKEPFESLPDNAFE +ADWERNLI DR+
Sbjct: 247 DPWKEPFESLPDNAFEDIADWERNLIWDRV 276

BLAST of MC09g1055 vs. ExPASy TrEMBL
Match: A0A6J1D0C4 (phosphoglucan phosphatase LSF2, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111015894 PE=4 SV=1)

HSP 1 Score: 584 bits (1505), Expect = 1.35e-210
Identity = 276/277 (99.64%), Postives = 277/277 (100.00%), Query Frame = 0

Query: 1   MATSSFPPSFSSLSVILPLQNHTPNKFLSFPSKLSKPHLQFHRICCGMPETGVRQNPTSG 60
           MATSSFPPSFSSLSVILPLQNHTPNKFLSFPSKLSKPHLQFHRICCGMPETGVRQNPTSG
Sbjct: 1   MATSSFPPSFSSLSVILPLQNHTPNKFLSFPSKLSKPHLQFHRICCGMPETGVRQNPTSG 60

Query: 61  GASTSRNSTEMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKPKDIDFLKEEE 120
           GASTSRNSTEMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKPKDIDFLKEEE
Sbjct: 61  GASTSRNSTEMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKPKDIDFLKEEE 120

Query: 121 GVAYILNLQQDKDVEYWGVDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLE 180
           GVAYILNLQQDKDVEYWGVDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLE
Sbjct: 121 GVAYILNLQQDKDVEYWGVDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLE 180

Query: 181 WAISEGKGKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGA 240
           WAISEGKGKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGA
Sbjct: 181 WAISEGKGKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGA 240

Query: 241 TYDLCKTDQWKEPFESLPDNAFEGVADWERNLIQDRL 277
           TYDLCKTDQWKEPFESLPDNAFEGVADWERNLIQDR+
Sbjct: 241 TYDLCKTDQWKEPFESLPDNAFEGVADWERNLIQDRV 277

BLAST of MC09g1055 vs. ExPASy TrEMBL
Match: A0A0A0KQM6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G218830 PE=4 SV=1)

HSP 1 Score: 482 bits (1241), Expect = 2.23e-170
Identity = 222/270 (82.22%), Postives = 248/270 (91.85%), Query Frame = 0

Query: 8   PSFSSLSVILPLQNHTPNKFLSFPSKLSKPHLQFHRICCGMPETGVRQNPTSGGASTSRN 67
           P FSSL  + PLQN+ PNKFL F S LSKP  +F+RICCG+PE+G++QNP++   S+S+N
Sbjct: 7   PCFSSLFGVFPLQNNAPNKFLPFSSILSKPLYRFNRICCGVPESGIQQNPSTSRPSSSKN 66

Query: 68  STEMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKPKDIDFLKEEEGVAYILN 127
             E YN+ MKKMM+NPYEYHHELGMNYTVIADNLIVGSQPQKP+DI+FLKEEEGVAYILN
Sbjct: 67  RMEDYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDINFLKEEEGVAYILN 126

Query: 128 LQQDKDVEYWGVDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLEWAISEGK 187
           LQQDKDVEYWG+DL+SIIERCKELGIHHMR+PA+DFDPDS+R GLPKAVSLLEWAIS+GK
Sbjct: 127 LQQDKDVEYWGIDLQSIIERCKELGIHHMRQPAKDFDPDSLRTGLPKAVSLLEWAISKGK 186

Query: 188 GKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLCKT 247
           GKVYVHCTAGLGRAPAVAIAYL+WFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDL K 
Sbjct: 187 GKVYVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLSKN 246

Query: 248 DQWKEPFESLPDNAFEGVADWERNLIQDRL 277
           D WKEPFESLPDNAFE +ADWERNLI+DR+
Sbjct: 247 DPWKEPFESLPDNAFEDIADWERNLIRDRV 276

BLAST of MC09g1055 vs. ExPASy TrEMBL
Match: A0A1S3C4E0 (phosphoglucan phosphatase LSF2, chloroplastic isoform X3 OS=Cucumis melo OX=3656 GN=LOC103496723 PE=4 SV=1)

HSP 1 Score: 474 bits (1219), Expect = 5.01e-167
Identity = 218/270 (80.74%), Postives = 245/270 (90.74%), Query Frame = 0

Query: 8   PSFSSLSVILPLQNHTPNKFLSFPSKLSKPHLQFHRICCGMPETGVRQNPTSGGASTSRN 67
           P FSSL  + PLQN+ PNK L F S LSKP  +F+RICCG+PE+G++QNP++   S+S+N
Sbjct: 7   PCFSSLFGVFPLQNNAPNKLLPFSSTLSKPLYRFNRICCGVPESGIQQNPSTSRPSSSKN 66

Query: 68  STEMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKPKDIDFLKEEEGVAYILN 127
             + YN+ MKKMM+NPYEYHHELGMNYTVIADNLIVGSQPQKP+DI+FLKEEEGVAYILN
Sbjct: 67  RMDEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDINFLKEEEGVAYILN 126

Query: 128 LQQDKDVEYWGVDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLEWAISEGK 187
           LQQDKDVEYWG++L+SIIERCKELGI HMR+PA+DFDPDS+R GLPKAVSLLEWAIS+GK
Sbjct: 127 LQQDKDVEYWGINLQSIIERCKELGIRHMRQPAKDFDPDSLRTGLPKAVSLLEWAISKGK 186

Query: 188 GKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLCKT 247
           GKVYVHCTAGLGRAPAVAIAYL+WFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDL K 
Sbjct: 187 GKVYVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLSKN 246

Query: 248 DQWKEPFESLPDNAFEGVADWERNLIQDRL 277
           D WKEPFESLPDNAFE +ADWERNLI DR+
Sbjct: 247 DPWKEPFESLPDNAFEDIADWERNLIWDRV 276

BLAST of MC09g1055 vs. ExPASy TrEMBL
Match: A0A1S3C3W8 (phosphoglucan phosphatase LSF2, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103496723 PE=4 SV=1)

HSP 1 Score: 474 bits (1219), Expect = 6.69e-167
Identity = 218/270 (80.74%), Postives = 245/270 (90.74%), Query Frame = 0

Query: 8   PSFSSLSVILPLQNHTPNKFLSFPSKLSKPHLQFHRICCGMPETGVRQNPTSGGASTSRN 67
           P FSSL  + PLQN+ PNK L F S LSKP  +F+RICCG+PE+G++QNP++   S+S+N
Sbjct: 7   PCFSSLFGVFPLQNNAPNKLLPFSSTLSKPLYRFNRICCGVPESGIQQNPSTSRPSSSKN 66

Query: 68  STEMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKPKDIDFLKEEEGVAYILN 127
             + YN+ MKKMM+NPYEYHHELGMNYTVIADNLIVGSQPQKP+DI+FLKEEEGVAYILN
Sbjct: 67  RMDEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDINFLKEEEGVAYILN 126

Query: 128 LQQDKDVEYWGVDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLEWAISEGK 187
           LQQDKDVEYWG++L+SIIERCKELGI HMR+PA+DFDPDS+R GLPKAVSLLEWAIS+GK
Sbjct: 127 LQQDKDVEYWGINLQSIIERCKELGIRHMRQPAKDFDPDSLRTGLPKAVSLLEWAISKGK 186

Query: 188 GKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLCKT 247
           GKVYVHCTAGLGRAPAVAIAYL+WFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDL K 
Sbjct: 187 GKVYVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLSKN 246

Query: 248 DQWKEPFESLPDNAFEGVADWERNLIQDRL 277
           D WKEPFESLPDNAFE +ADWERNLI DR+
Sbjct: 247 DPWKEPFESLPDNAFEDIADWERNLIWDRV 276

BLAST of MC09g1055 vs. ExPASy TrEMBL
Match: A0A1S3C5J9 (phosphoglucan phosphatase LSF2, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496723 PE=4 SV=1)

HSP 1 Score: 473 bits (1218), Expect = 9.85e-167
Identity = 218/269 (81.04%), Postives = 244/269 (90.71%), Query Frame = 0

Query: 8   PSFSSLSVILPLQNHTPNKFLSFPSKLSKPHLQFHRICCGMPETGVRQNPTSGGASTSRN 67
           P FSSL  + PLQN+ PNK L F S LSKP  +F+RICCG+PE+G++QNP++   S+S+N
Sbjct: 7   PCFSSLFGVFPLQNNAPNKLLPFSSTLSKPLYRFNRICCGVPESGIQQNPSTSRPSSSKN 66

Query: 68  STEMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKPKDIDFLKEEEGVAYILN 127
             + YN+ MKKMM+NPYEYHHELGMNYTVIADNLIVGSQPQKP+DI+FLKEEEGVAYILN
Sbjct: 67  RMDEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDINFLKEEEGVAYILN 126

Query: 128 LQQDKDVEYWGVDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLEWAISEGK 187
           LQQDKDVEYWG++L+SIIERCKELGI HMR+PA+DFDPDS+R GLPKAVSLLEWAIS+GK
Sbjct: 127 LQQDKDVEYWGINLQSIIERCKELGIRHMRQPAKDFDPDSLRTGLPKAVSLLEWAISKGK 186

Query: 188 GKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLCKT 247
           GKVYVHCTAGLGRAPAVAIAYL+WFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDL K 
Sbjct: 187 GKVYVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLSKN 246

Query: 248 DQWKEPFESLPDNAFEGVADWERNLIQDR 276
           D WKEPFESLPDNAFE +ADWERNLI DR
Sbjct: 247 DPWKEPFESLPDNAFEDIADWERNLIWDR 275

BLAST of MC09g1055 vs. TAIR 10
Match: AT3G10940.1 (dual specificity protein phosphatase (DsPTP1) family protein )

HSP 1 Score: 367.5 bits (942), Expect = 1.0e-101
Identity = 172/259 (66.41%), Postives = 209/259 (80.69%), Query Frame = 0

Query: 20  QNHTPNKFLSFPSKLSKPHLQFHRICCGMPETGVRQNPTSGGAS-TSRNSTEMYNMTMKK 79
           +N   + F S  +K S   L+F R   G+      +NP + G S +S+N  E YN  MK+
Sbjct: 19  ENEKSSCFTSI-NKKSSLDLRFPRNLAGVSCKFSGENPGTNGVSLSSKNKMEDYNTAMKR 78

Query: 80  MMKNPYEYHHELGMNYTVIADNLIVGSQPQKPKDIDFLKEEEGVAYILNLQQDKDVEYWG 139
           +M++PYEYHH+LGMNYT+I D LIVGSQPQKP+DID LK+E+ VAYILNLQQDKD+EYWG
Sbjct: 79  LMRSPYEYHHDLGMNYTLIRDELIVGSQPQKPEDIDHLKQEQNVAYILNLQQDKDIEYWG 138

Query: 140 VDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLEWAISEGKGKVYVHCTAGL 199
           +DL SI+ RCKELGI HMR+PA+DFDP S+R+ LPKAVS LEWA+SEGKG+VYVHC+AGL
Sbjct: 139 IDLDSIVRRCKELGIRHMRRPAKDFDPLSLRSQLPKAVSSLEWAVSEGKGRVYVHCSAGL 198

Query: 200 GRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLCKTDQWKEPFESLP 259
           GRAP V+IAY++WFC MNLNTAY+ L SKRPCGP+K AIRGATYDL K D WKEPFESLP
Sbjct: 199 GRAPGVSIAYMYWFCDMNLNTAYDTLVSKRPCGPNKGAIRGATYDLAKNDPWKEPFESLP 258

Query: 260 DNAFEGVADWERNLIQDRL 278
           +NAFE +ADWER LIQ+R+
Sbjct: 259 ENAFEDIADWERKLIQERV 276

BLAST of MC09g1055 vs. TAIR 10
Match: AT3G52180.1 (dual specificity protein phosphatase (DsPTP1) family protein )

HSP 1 Score: 156.8 bits (395), Expect = 2.8e-38
Identity = 90/206 (43.69%), Postives = 115/206 (55.83%), Query Frame = 0

Query: 61  GASTSRNSTEMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKPKDIDFLKEEE 120
           G +     ++ Y+  M + M     Y HELGMNY  I  +LIVGS  Q P+D+D L+ + 
Sbjct: 65  GVAKDEEKSDEYSQDMTQAMGAVLTYRHELGMNYNFIRPDLIVGSCLQTPEDVDKLR-KI 124

Query: 121 GVAYILNLQQDKDVEYWGVDLRSIIERCKELG-IHHMRKPARDFDPDSMRNGLPKAVSLL 180
           GV  I  LQQD D+EY+GVD+ SI    K+   I H+R   RDFD   +R  LP  V  L
Sbjct: 125 GVKTIFCLQQDPDLEYFGVDISSIQAYAKKYSDIQHIRCEIRDFDAFDLRMRLPAVVGTL 184

Query: 181 EWAISEGKGKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRG 240
             A+    G  YVHCTAG+GRAPAVA+ Y+FW  G  L  A++ L SKR C P   AIR 
Sbjct: 185 YKAVKRNGGVTYVHCTAGMGRAPAVALTYMFWVQGYKLMEAHKLLMSKRSCFPKLDAIRN 244

Query: 241 ATYDLCKTDQWKEPFESLPDNAFEGV 266
           AT D+    + K    +L D  F  V
Sbjct: 245 ATIDILTGLKRKTVTLTLKDKGFSRV 269

BLAST of MC09g1055 vs. TAIR 10
Match: AT3G52180.2 (dual specificity protein phosphatase (DsPTP1) family protein )

HSP 1 Score: 156.8 bits (395), Expect = 2.8e-38
Identity = 90/206 (43.69%), Postives = 115/206 (55.83%), Query Frame = 0

Query: 61  GASTSRNSTEMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKPKDIDFLKEEE 120
           G +     ++ Y+  M + M     Y HELGMNY  I  +LIVGS  Q P+D+D L+ + 
Sbjct: 65  GVAKDEEKSDEYSQDMTQAMGAVLTYRHELGMNYNFIRPDLIVGSCLQTPEDVDKLR-KI 124

Query: 121 GVAYILNLQQDKDVEYWGVDLRSIIERCKELG-IHHMRKPARDFDPDSMRNGLPKAVSLL 180
           GV  I  LQQD D+EY+GVD+ SI    K+   I H+R   RDFD   +R  LP  V  L
Sbjct: 125 GVKTIFCLQQDPDLEYFGVDISSIQAYAKKYSDIQHIRCEIRDFDAFDLRMRLPAVVGTL 184

Query: 181 EWAISEGKGKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRG 240
             A+    G  YVHCTAG+GRAPAVA+ Y+FW  G  L  A++ L SKR C P   AIR 
Sbjct: 185 YKAVKRNGGVTYVHCTAGMGRAPAVALTYMFWVQGYKLMEAHKLLMSKRSCFPKLDAIRN 244

Query: 241 ATYDLCKTDQWKEPFESLPDNAFEGV 266
           AT D+    + K    +L D  F  V
Sbjct: 245 ATIDILTGLKRKTVTLTLKDKGFSRV 269

BLAST of MC09g1055 vs. TAIR 10
Match: AT3G01510.1 (like SEX4 1 )

HSP 1 Score: 111.3 bits (277), Expect = 1.3e-24
Identity = 59/164 (35.98%), Postives = 88/164 (53.66%), Query Frame = 0

Query: 81  KNPYEYHHELGMNYTVIADNLIVGSQPQKPKDIDFLKEEEGVAYILNLQQDKDVEYWGVD 140
           K    Y+H LGM Y+ I + + VGS  Q  +D++ L  E G+  ILN Q   + + WG+D
Sbjct: 279 KGELSYNHALGMRYSKITEQIYVGSCIQTEEDVENL-SEAGITAILNFQGGTEAQNWGID 338

Query: 141 LRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLEWAISEGKGKVYVHCTAGLGR 200
            +SI + C++  +  +  P +D D   +R  LP  V LL   + +   +V+V CT G  R
Sbjct: 339 SQSINDACQKSEVLMINYPIKDADSFDLRKKLPLCVGLLLRLLKKNH-RVFVTCTTGFDR 398

Query: 201 APAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDL 245
           + A  IAYL W    +L+ AY  +T    C P + AI  AT+DL
Sbjct: 399 SSACVIAYLHWMTDTSLHAAYSFVTGLHACKPDRPAIAWATWDL 440

BLAST of MC09g1055 vs. TAIR 10
Match: AT3G55270.1 (mitogen-activated protein kinase phosphatase 1 )

HSP 1 Score: 42.4 bits (98), Expect = 7.7e-04
Identity = 16/46 (34.78%), Postives = 29/46 (63.04%), Query Frame = 0

Query: 183 ISEGKGKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKR 229
           + E  G+++VHC  G+ R+ ++ IAYL W  G + + A++ + S R
Sbjct: 224 VREQSGRIFVHCCQGVSRSTSLVIAYLMWREGQSFDDAFQYVKSAR 269

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SRK51.5e-10066.41Phosphoglucan phosphatase LSF2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9FEB53.9e-3743.69Phosphoglucan phosphatase DSP4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
G4LTX41.5e-3641.07Phosphoglucan phosphatase DSP4, amyloplastic OS=Castanea sativa OX=21020 GN=DSP4... [more]
F4J1171.9e-2335.98Phosphoglucan phosphatase LSF1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
Q1M1992.8e-1129.88Laforin OS=Canis lupus familiaris OX=9615 GN=EPM2A PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022146766.12.79e-21099.64phosphoglucan phosphatase LSF2, chloroplastic [Momordica charantia] >XP_02214676... [more]
XP_011655031.14.61e-17082.22phosphoglucan phosphatase LSF2, chloroplastic [Cucumis sativus] >XP_011655032.1 ... [more]
XP_038891555.13.10e-16980.58phosphoglucan phosphatase LSF2, chloroplastic [Benincasa hispida][more]
XP_008456918.11.03e-16680.74PREDICTED: phosphoglucan phosphatase LSF2, chloroplastic isoform X3 [Cucumis mel... [more]
XP_008456916.11.38e-16680.74PREDICTED: phosphoglucan phosphatase LSF2, chloroplastic isoform X2 [Cucumis mel... [more]
Match NameE-valueIdentityDescription
A0A6J1D0C41.35e-21099.64phosphoglucan phosphatase LSF2, chloroplastic OS=Momordica charantia OX=3673 GN=... [more]
A0A0A0KQM62.23e-17082.22Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G218830 PE=4 SV=1[more]
A0A1S3C4E05.01e-16780.74phosphoglucan phosphatase LSF2, chloroplastic isoform X3 OS=Cucumis melo OX=3656... [more]
A0A1S3C3W86.69e-16780.74phosphoglucan phosphatase LSF2, chloroplastic isoform X2 OS=Cucumis melo OX=3656... [more]
A0A1S3C5J99.85e-16781.04phosphoglucan phosphatase LSF2, chloroplastic isoform X1 OS=Cucumis melo OX=3656... [more]
Match NameE-valueIdentityDescription
AT3G10940.11.0e-10166.41dual specificity protein phosphatase (DsPTP1) family protein [more]
AT3G52180.12.8e-3843.69dual specificity protein phosphatase (DsPTP1) family protein [more]
AT3G52180.22.8e-3843.69dual specificity protein phosphatase (DsPTP1) family protein [more]
AT3G01510.11.3e-2435.98like SEX4 1 [more]
AT3G55270.17.7e-0434.78mitogen-activated protein kinase phosphatase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020422Dual specificity protein phosphatase domainSMARTSM00195dsp_5coord: 93..243
e-value: 1.8E-4
score: 21.8
IPR020422Dual specificity protein phosphatase domainPROSITEPS50054TYR_PHOSPHATASE_DUALcoord: 93..253
score: 28.037785
IPR000340Dual specificity phosphatase, catalytic domainPFAMPF00782DSPccoord: 116..230
e-value: 8.3E-16
score: 58.0
IPR029021Protein-tyrosine phosphatase-likeGENE3D3.90.190.10Protein tyrosine phosphatase superfamilycoord: 76..280
e-value: 1.1E-66
score: 226.2
IPR029021Protein-tyrosine phosphatase-likeSUPERFAMILY52799(Phosphotyrosine protein) phosphatases IIcoord: 85..233
NoneNo IPR availablePANTHERPTHR46642:SF2PHOSPHOGLUCAN PHOSPHATASE LSF2, CHLOROPLASTICcoord: 33..277
NoneNo IPR availablePANTHERPTHR46642DUAL SPECIFICITY PHOSPHATASE, SUBGROUP, CATALYTIC DOMAINcoord: 33..277
NoneNo IPR availableCDDcd14526DSP_laforin-likecoord: 92..239
e-value: 3.10027E-79
score: 235.168
IPR000387Tyrosine specific protein phosphatases domainPROSITEPS50056TYR_PHOSPHATASE_2coord: 170..229
score: 11.5674

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g1055.1MC09g1055.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046838 phosphorylated carbohydrate dephosphorylation
biological_process GO:0006470 protein dephosphorylation
biological_process GO:0005983 starch catabolic process
biological_process GO:0016311 dephosphorylation
cellular_component GO:0009507 chloroplast
molecular_function GO:0019203 carbohydrate phosphatase activity
molecular_function GO:0008138 protein tyrosine/serine/threonine phosphatase activity
molecular_function GO:2001070 starch binding
molecular_function GO:0016791 phosphatase activity