MC09g0399 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g0399
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionribulose bisphosphate carboxylase/oxygenase activase, chloroplastic
LocationMC09: 3603489 .. 3609868 (-)
RNA-Seq ExpressionMC09g0399
SyntenyMC09g0399
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAATCTCTATATCCGGTCTCTCCCTTCCCTCACTCTTTCAGTACAAAAATTCAACAATCGCGCGCGAAATCCGAATTCGTTCCTTCGCCATTCGATCTTCGAAGGATGTTCCTGGTGAAACCAGCGGCGGCGGCGACGGCGAGCCGTCGGAGAGGCTGAAGAGACTGTCGGAGCAGTCTTCCTGGGAAGCCAAGGACTCCGAGGGCCGGGACTATCTCTATAGGCTTGGCAATGAAGCAGATAACATGAACATCGCCGTCGGAGCTAGAGCTGGCGTTATCGACGACCTTTTCGCCGGCAACTTCCTCGGCAGAGACTGTAAGTGTCCGATTTATCTGTGTTCTTTATTTGTCTATTTGTGGTTTTGCTTTCAGTTTTATTAGTGTTTTTTTTGTGTGTTGAATTTTGTTTTGTAGCGGACATTGTGTTTGATTACCGCCATAAGGTCACAAGATCGTTTGAGTACCTTCAAGGAGACTATTACATCGCTCCTGTTTTCATGGTAATTTCCGCCTCAGTTTTTTTTAGTTATTGATTATTTTTCTTGTTTCTTCATTGTTGTTCAATTGAATTTATATTTTCTGTAACTGCAACGTCGACCGATTTCGTGCTCATTGATTTTGAATCATTTATCCCAGTACTTGGAATCGAAATGTGCCTGATGACTTTAACATTACAATTCGAGTAGAAACGTCCTTTCCAACTCTGTCTTCATTATTGTTTAGTTGAATTATATTTTCTATAACTGCAACCAACTTTGACAGATTGTGCGCTCCATGATTTTGAAACATTTAATTCCTATTCTTGGAATCGAAATAATTGAAACGATGACAACAGAATTATTATGGTAGAAATGTGGACGTTTCCACCTTGGGCAAATCGCTGCATTTTCTTTTTGTTGTTTTTTTTCCAAAATCTTTTCTATTTGTTATTTCTAATCTCTTTTGTTGCTTTTCAAGAAGTATGCGCAGGGTTATGGTCCACGTGTCATATGTCAGTAGCATTCAGCCTTTTTACGTTTTTATTTATTTTTATGTTTCTGATATATTTTGTGTTATAATTTGAGAATGATCATCTCTGCAATAATCTGTCTTCTTACACTTGTTTCATTGATTTTTTCTTTTGACAGGATAAAGTTGGTAAGTTCATAGAAACTTGGTGTTGATAAACGACATATGATGAATTACGTTTGAAGAAGTAAGAGACTTCAAAATATATGTGATGTAGTCAACTTCATTTGTTGCATGATTTTTAAATCCGAACAGTTATCAATTGCTATAAGATGTAAGTTCATATTTAAGTCTAAAAAATTAGTTAGATAAAACCAATCAAACTGGAATTTTCAATTTCAACGATTTATGCATATAAAATTTGTTCAGTCTTGTGTCTTCCTGTACTAAGCACTAATAGTAACCCACATCATCGATGTAAAGTGTGTTTACACAAGCCTGGAATCCCTGACATTTCTATATACCCCATTACCATTACTATTTGATGTTGTATGATTTGTAGGACCTGGAGAACAACAGGTAATTATCACTAGTTGAAACCCAGATCTTAATTTGTGATATATAATTTTGAGATTTTAGAAAAGTAGATCCTTCTAAGCAGTTTCATCAACTACAGCTTCTATAACAATTGATCTGGCCACAAAATCAACAAAAATCCCCATTGTTGGATAAGTCATTATTTCATATTATATGCTACATATTTGCCATCAGATTGTTTAAACTCTTTCTGTCTTTGATAGTGTTTTGTTGTAATGTTTTTGAACTCCGCCTATAGATGTAATTAGCATTTTTCTGCTGATTGTAAGCTTAATATTTTGGTTGTAATCTAACTGGTTAGGCAGTCTCATGGATGTCCTTCTCTTTAGGAAGAGAAATGCAGCATGAAGATATGTTGGTAGGGCTTCTCTTTAGGAATTTAAGACGAAGGAGTTTTGCAGGAAGACTCTACCTGCTTTCATGAAATGAGTACAAACAAGTACCAGTTAAGTTTAACCTGCGTTAACATAGTCTGTGTTGTTGATGCAGTATGCCACATTGTGAAGAACTTCATAGCTCACCTTCTCAATATCAAAGTTCCTCTCATCCTAGGTGTGTATTAATTTATTTTACCAGTTTCTATACAAATTCTTTGATAGTCAAATTAATAGAACATGATGTTAAAAACCTATTCGTGAAAAGTTCATCAATTTATGTGTCAATGGGAGTCTGTTCCATTGCGTTGAATCTTCATCAGGTTTCCTCTTTTTAGGATGGAATATTGTCCTAATCAATCTTGACATGTGTAGCTTCATGATAGTGCTAAAACATTATCAAAGAATTTGTATAACTCCGGTGATGTGAACACGCGTCTCTCCTTTTACCTATTCTAAAAAAAAATTATTTTATGATTTATCTATTAGATTTAATGAATTCTATCTTCATTACTTCCACATTTTTCCATAAAAGATGACTGCATAGTTGGAAATAAAAGAATTTTGTTTCACAGGTATTTGGGGAGGAAAGGGTCAAGGAAAGTCATTTCAGACTGAACTTATATTCCAGACCATGGGAGTGGAACCCGTTATAATGTCTGCTGGAGAATTAGAATCTGAAAGGGCTGGTAAGATAGGGACCTGGGATGACTTATTTTAACTTGTGCGTCCTTAATTTGATGGTGGATTTAAAGCATTCCATATTCTTTAATTAGCTTAATCTGGATATTATTTTGTAAATTAGCTAAGAAAAACGTAATTTTACGGACTAATATTCTCTCAATTGTAACTTAATTGTAGTCGTTCTTTGAGTAAAGCATACATCAATAGGTCAAACTCATGCAGTTATTCATTGCAAATTTGTACGCCATTTCCTTAAGTGAGAAATCTGGTTCTATGTATGGACAAGTCCATATATTTTGTATTCCGATATTGGGGTGGAAATTGGAATGCCATGTTAAATTGTGATTTTCATCAACAGGTGAGCCAGGAAAACTGATACGTGAACGCTATAGAACTGCTTCTCAAGTGATCCAGAATCAAGTTAGAAGCTTTACCACTTACATTCTAGATATATGTATGTCTTGGCTTAAATGTTGCTTCGTGCAACTTTATGTGCACAATGTTCCATATTTTCATATAAAACACCCTTATTCTTCAGTGCCAAAGTGTTATCATTATAGATCGTCTGTTGTAGAATATATTTAATGATTTGACTGTAAGTGCCCTGTGAAAAACCTTGAATTGTATAGACAACATTAGCGAAGGAAGATGAAGTCTAGAAAGTAGCTTTCTTGAGTCTACTATTACTTTATGTCCTCAGTAACAGGTAGCGATGAAGTCTAGAAAGTAGTTCTGATGAGTTGTCTATTATTTTAAGCAGCTTCTTGTGATTTAACACAGCTTTGAATTTGATTGAACTTTCTCATACGTGCCAGTTTCCTTTTCTGTAACATTAGTTACCTCATTTGATCTTGCAAATCTGAAATTTTAATTTAGACGTGTATTTTAAGTGTCAAAAATCACTGGTCATTTTGTGGTCTACTCCATCATTTTGGTCTTACCTTTACATATCTGAAAGATCAGTTTACTTTTCATATGGTTTCTCTTAAAAGTTAATGTGCAATGATTTGGTGTTCTTGTAAAATTATGTTTATTTTGCATTTATTACTTTTCCTGTTAATATTATTTATGATTTCCTCTGGGTCCCTTTTTTCTTTTAAAATATATTATGGCATGAGGTGCAAAGTGCAAGACGATGCTTAATTAGTAGATGAATATATAAAAGATGCTTCATGATTGAAAATCATAATAAGACACATGCTTGTTGCTCTCACTGGCCATAAGGTATATGCACATGCCTCTCAGGTGCACCTTGAACAACTCTGTACTTAGTTGGTCATGCTTAAGCTTGATCCAAAAACCTTTTAGTGTTTTTTCATAGTTATTCTAAAATTAACTTCAATGCATTCAGAAATAATTCACGTATGACCTGGGGACTTTTTTTTTACCACTGCAGGGGAAATTGAGTTGTTTGATGATCAATGATATTGATGCTGGCATTGGTAGATTTGGTAAGATTCTATGTACTTGCTCCTGAACATAAAAAATTTGATAAGTAATAATAAAAATGATGTGAATGCATCATCTGTGACCCCTTAGATAATACAAATTGACATTTGGAGTTAAAATTTATTGTATTGTGCGTTAAAATGATTATTCAATCATATATAACACATATGTTTATATTTTGTTCTTATCTAGGCAATACACAGGTGACAGTCAACAACCAAATTGCTGCTGGAACTCTGATGAATCTTGCTGACAATCCTACCAGAGTGAGCATTGGACAAGATTGGCGAGAATCAGATATTACGAACAGAATTCCAATCATTCTTACAGGAAATGATTTTTCAACTATTTATGCTCCCTTAATTCGTGATGGCAGGATGGAGAAATTCTACTGGTATGAAATTGATGGTACAAGAATTGATGTGCTGTTGACTTCCTGAAGATGTTTTAATAAATTGTACTAGTTTCTCCAGATCTAGCTCTGTGATCATATTTGGATCCAAATGGATGAGCTACATTTTTATACTTGTGATTCTCGCACTTTTTTCTTTCAAGTCATAAATAGTATTAAAGCTAAGGCACTCTTCTCTCAAATGTTGTTTGGTAACAGGCAACCTAACCGTGAAGATATCGTGAATATTGTTCATAGAATGTATGAGAAAGATGGCATATCAAGGTCTCAGGTCATTGACATTGTAAATACTTTTCCTAATCAAGGTAGGTTCCTTTTTCATGTCATGTTTAAAGAACAAGTGTGTGGAAATCGACTCCTTTTTGAGTGAAATATAACTTAATTTGAATGTGTCATTATAATTTTGCATTTAGAAATTGCTCTTCTTTCCTCCTTTCACAGCTTTGGACTTTTATGGAGCTCTGAGGTCACGGACATATGATGCAGCAGTATCTAAGGTGTATAACACTTTCATTGACATCTGTAAGACCTTGATAAATAATATTAATGTTTCTTCCGAAGTATGTATGGAATGAAGATGATAGGAACCATTCCATTTATTTGAAAAGCATAATTAATAGCATGTCATAAATGAAGGTTTTTTCTTCTTTTTTTTCCTACAATTAATGTGTGTAAATTAATCCTTTGATCGAACTCCCTTTTTTCATTTCAGTGGGTTGATGATATTGGAGGCGTGGAAAAGCTGGGAGATAAGCTGCTTAAGAGAAGGAAGAATGAAAAGCTTCCTACATTCACACCACCAGAGGTGGTCAATCTATAAAATGTTCAGATTCGTGCTTTGCACATTCACATGTTGATTCATGAATTGTTTATTTATAATTCAGTATGCTAGAACTAGAATAGATAAAACTTAATATCACATTTAAGTGAGTCATAAAGCTCTCTAAACAAGTCTTGCATGATAATTCACAGCTGATCTGTATCCTTGGTTGACCTGCGTGATCATTATTCACCTTATTTATGGCATTCCAACTGTTTATCCCTGAATCTTTAGTAATGACTAGTTGTATGTCTCAGACATATGGAGTATTTAGGAAGTATATATGTTAATTTATCATCTAAAATTTGGAGTTCCACAATGAATCTTTCCTATTAGTCTTCTGGAGAAACCACATTTTGTCGGGCAGTATATTGTCTGCATTTACGAAAAGTTGAAGGAACAGAACTCACGACTTTCGTTGAAAGTTGTATATAGTCTTTCATGCTCTTTTCTTTATTATTTATTGCAGAGCGAAGTGAAGTATGTTATTCACTACTTTCGTGTCTATGTTCTGAATATGTAAACTTAAAGTAAGTTCACTTGGAGTGACTTTTTTGTTTGGTTGAGCAGCAAACAATGGAGGCATTGCTTGAAGCAGGCTATTCTCTTGTTAAAGAACAGCAATTGATCATGGAGACTAAACTTTCAAAAGAATACATGAAGAACATGGACGACTAGCCCAAAATGTGAGATAGCGACCTAAATTTGTTTGTGATATCTGTTCATATCACTTTTTAAGCAAATGCCAATACATGTATTTTTTTTTTTCACAGAATTTGTTATTTGTTCTGTTTTCTTTTGGGATCACATTAGAACCAAGCATTTACGCTTCACATTGTTCATTATTGCAAATTAGTGTTATATTCACACGACTACGACCTTTATAGTTTGTTATGTCAATAGATCCGTTAGTCAGAGTGAAACTTTATTATCCACTGTCATATTCAGCCGGCCCTTTGGATGATTTGTCTCGAAACTTACCTGTTCATCTTGTCATTATAATATTTTGATCTCATTTTGTTGATATTATTATTACTTCAAAAAACATCCTTCTGACTGATTGCCTCGTTGGATATCGTATACGAAATAG

mRNA sequence

ATGGCAATCTCTATATCCGGTCTCTCCCTTCCCTCACTCTTTCAGTACAAAAATTCAACAATCGCGCGCGAAATCCGAATTCGTTCCTTCGCCATTCGATCTTCGAAGGATGTTCCTGGTGAAACCAGCGGCGGCGGCGACGGCGAGCCGTCGGAGAGGCTGAAGAGACTGTCGGAGCAGTCTTCCTGGGAAGCCAAGGACTCCGAGGGCCGGGACTATCTCTATAGGCTTGGCAATGAAGCAGATAACATGAACATCGCCGTCGGAGCTAGAGCTGGCGTTATCGACGACCTTTTCGCCGGCAACTTCCTCGGCAGAGACTCGGACATTGTGTTTGATTACCGCCATAAGGTCACAAGATCGTTTGAGTACCTTCAAGGAGACTATTACATCGCTCCTGTTTTCATGGATAAAGTTGTATGCCACATTGTGAAGAACTTCATAGCTCACCTTCTCAATATCAAAGTTCCTCTCATCCTAGGTATTTGGGGAGGAAAGGGTCAAGGAAAGTCATTTCAGACTGAACTTATATTCCAGACCATGGGAGTGGAACCCGTTATAATGTCTGCTGGAGAATTAGAATCTGAAAGGGCTGGTGAGCCAGGAAAACTGATACGTGAACGCTATAGAACTGCTTCTCAAGTGATCCAGAATCAAGGGAAATTGAGTTGTTTGATGATCAATGATATTGATGCTGGCATTGGTAGATTTGGCAATACACAGGTGACAGTCAACAACCAAATTGCTGCTGGAACTCTGATGAATCTTGCTGACAATCCTACCAGAGTGAGCATTGGACAAGATTGGCGAGAATCAGATATTACGAACAGAATTCCAATCATTCTTACAGGAAATGATTTTTCAACTATTTATGCTCCCTTAATTCGTGATGGCAGGATGGAGAAATTCTACTGGCAACCTAACCGTGAAGATATCGTGAATATTGTTCATAGAATGTATGAGAAAGATGGCATATCAAGGTCTCAGGTCATTGACATTGTAAATACTTTTCCTAATCAAGCTTTGGACTTTTATGGAGCTCTGAGGTCACGGACATATGATGCAGCAGTATCTAAGTGGGTTGATGATATTGGAGGCGTGGAAAAGCTGGGAGATAAGCTGCTTAAGAGAAGGAAGAATGAAAAGCTTCCTACATTCACACCACCAGAGCAAACAATGGAGGCATTGCTTGAAGCAGGCTATTCTCTTGTTAAAGAACAGCAATTGATCATGGAGACTAAACTTTCAAAAGAATACATGAAGAACATGGACGACTAGCCCAAAATGTGAGATAGCGACCTAAATTTGTTTGTGATATCTGTTCATATCACTTTTTAAGCAAATGCCAATACATGTATTTTTTTTTTTCACAGAATTTGTTATTTGTTCTGTTTTCTTTTGGGATCACATTAGAACCAAGCATTTACGCTTCACATTGTTCATTATTGCAAATTAGTGTTATATTCACACGACTACGACCTTTATAGTTTGTTATGTCAATAGATCCGTTAGTCAGAGTGAAACTTTATTATCCACTGTCATATTCAGCCGGCCCTTTGGATGATTTGTCTCGAAACTTACCTGTTCATCTTGTCATTATAATATTTTGATCTCATTTTGTTGATATTATTATTACTTCAAAAAACATCCTTCTGACTGATTGCCTCGTTGGATATCGTATACGAAATAG

Coding sequence (CDS)

ATGGCAATCTCTATATCCGGTCTCTCCCTTCCCTCACTCTTTCAGTACAAAAATTCAACAATCGCGCGCGAAATCCGAATTCGTTCCTTCGCCATTCGATCTTCGAAGGATGTTCCTGGTGAAACCAGCGGCGGCGGCGACGGCGAGCCGTCGGAGAGGCTGAAGAGACTGTCGGAGCAGTCTTCCTGGGAAGCCAAGGACTCCGAGGGCCGGGACTATCTCTATAGGCTTGGCAATGAAGCAGATAACATGAACATCGCCGTCGGAGCTAGAGCTGGCGTTATCGACGACCTTTTCGCCGGCAACTTCCTCGGCAGAGACTCGGACATTGTGTTTGATTACCGCCATAAGGTCACAAGATCGTTTGAGTACCTTCAAGGAGACTATTACATCGCTCCTGTTTTCATGGATAAAGTTGTATGCCACATTGTGAAGAACTTCATAGCTCACCTTCTCAATATCAAAGTTCCTCTCATCCTAGGTATTTGGGGAGGAAAGGGTCAAGGAAAGTCATTTCAGACTGAACTTATATTCCAGACCATGGGAGTGGAACCCGTTATAATGTCTGCTGGAGAATTAGAATCTGAAAGGGCTGGTGAGCCAGGAAAACTGATACGTGAACGCTATAGAACTGCTTCTCAAGTGATCCAGAATCAAGGGAAATTGAGTTGTTTGATGATCAATGATATTGATGCTGGCATTGGTAGATTTGGCAATACACAGGTGACAGTCAACAACCAAATTGCTGCTGGAACTCTGATGAATCTTGCTGACAATCCTACCAGAGTGAGCATTGGACAAGATTGGCGAGAATCAGATATTACGAACAGAATTCCAATCATTCTTACAGGAAATGATTTTTCAACTATTTATGCTCCCTTAATTCGTGATGGCAGGATGGAGAAATTCTACTGGCAACCTAACCGTGAAGATATCGTGAATATTGTTCATAGAATGTATGAGAAAGATGGCATATCAAGGTCTCAGGTCATTGACATTGTAAATACTTTTCCTAATCAAGCTTTGGACTTTTATGGAGCTCTGAGGTCACGGACATATGATGCAGCAGTATCTAAGTGGGTTGATGATATTGGAGGCGTGGAAAAGCTGGGAGATAAGCTGCTTAAGAGAAGGAAGAATGAAAAGCTTCCTACATTCACACCACCAGAGCAAACAATGGAGGCATTGCTTGAAGCAGGCTATTCTCTTGTTAAAGAACAGCAATTGATCATGGAGACTAAACTTTCAAAAGAATACATGAAGAACATGGACGACTAG

Protein sequence

MAISISGLSLPSLFQYKNSTIAREIRIRSFAIRSSKDVPGETSGGGDGEPSERLKRLSEQSSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRHKVTRSFEYLQGDYYIAPVFMDKVVCHIVKNFIAHLLNIKVPLILGIWGGKGQGKSFQTELIFQTMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLSCLMINDIDAGIGRFGNTQVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIILTGNDFSTIYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQALDFYGALRSRTYDAAVSKWVDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQQLIMETKLSKEYMKNMDD
Homology
BLAST of MC09g0399 vs. ExPASy Swiss-Prot
Match: P93431 (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=RCA PE=1 SV=2)

HSP 1 Score: 339.7 bits (870), Expect = 4.7e-92
Identity = 177/334 (52.99%), Postives = 230/334 (68.86%), Query Frame = 0

Query: 93  GVIDDLF-AGNFLGRDSDIVFDYRHKV----TRSFEYLQGDYYIAPVFMDKVVCHIVKNF 152
           G +D LF A    G    ++  Y +      T  F+   G +YIAP FMDK+V HI KNF
Sbjct: 81  GFVDSLFQAPTGDGTHEAVLSSYEYLSQGLRTYDFDNTMGGFYIAPAFMDKLVVHISKNF 140

Query: 153 IAHLLNIKVPLILGIWGGKGQGKSFQTELIFQTMGVEPVIMSAGELESERAGEPGKLIRE 212
           +  L NIKVPLILGIWGGKGQGKSFQ EL+F  MG+ P++MSAGELES  AGEP KLIR+
Sbjct: 141 MT-LPNIKVPLILGIWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQ 200

Query: 213 RYRTASQVIQNQGKLSCLMINDIDAGIGRF-GNTQVTVNNQIAAGTLMNLADNPTRVSIG 272
           RYR A+ +I+ +GK+ CL IND+DAG GR  G TQ TVNNQ+   TLMN+ADNPT V + 
Sbjct: 201 RYREAADIIK-KGKMCCLFINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLP 260

Query: 273 QDWRESDITNRIPIILTGNDFSTIYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGIS 332
             + + D   R+PII+TGNDFST+YAPLIRDGRMEKFYW P R+D V +   ++  D + 
Sbjct: 261 GMYNKED-NPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTRDDRVGVCKGIFRTDNVP 320

Query: 333 RSQVIDIVNTFPNQALDFYGALRSRTYDAAVSKWVDDIGGVEKLGDKLLKRRKNEKLPTF 392
              ++ IV++FP Q++DF+GALR+R YD  V KWV D  GVE +G +L+  R  E  P F
Sbjct: 321 DEDIVKIVDSFPGQSIDFFGALRARVYDDEVRKWVSDT-GVENIGKRLVNSR--EGPPEF 380

Query: 393 TPPEQTMEALLEAGYSLVKEQQLIMETKLSKEYM 421
             P+ T+E L+E GY LVKEQ+ +   +L+++Y+
Sbjct: 381 EQPKMTIEKLMEYGYMLVKEQENVKRVQLAEQYL 408

BLAST of MC09g0399 vs. ExPASy Swiss-Prot
Match: O49074 (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Solanum pennellii OX=28526 PE=2 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 3.9e-91
Identity = 200/430 (46.51%), Postives = 269/430 (62.56%), Query Frame = 0

Query: 1   MAISIS--GLSLPSLFQYKNSTIAREIRIRSFAIRSSKDVPGETSGGGDGEPSERLKRLS 60
           MA S+S  G +  +     NS     +   +F  +S K V     G    + S R  R+ 
Sbjct: 1   MAASVSTIGAASKAPLSLNNSVAGTSVPSTAFFGKSLKKV--YAKGVSSPKVSNRNLRVV 60

Query: 61  EQSSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLF-AGNFLGRDSDIVFDYRHK 120
            Q   E K+   +  LY      D  +IA G   G++D LF A    G    I+  Y + 
Sbjct: 61  AQEVDETKEDRWKG-LYD-NTSDDQQDIARG--KGLVDSLFQAPTGTGTHHAIMNSYEY- 120

Query: 121 VTRSFEYLQGD-----YYIAPVFMDKVVCHIVKNFIAHLLNIKVPLILGIWGGKGQGKSF 180
           V+++ +  Q D     +YIAP FMDK+V HI KNF+  L NIKVPLILG+WGGKGQGKSF
Sbjct: 121 VSQALKTYQLDNKLDGFYIAPAFMDKLVVHITKNFLT-LPNIKVPLILGVWGGKGQGKSF 180

Query: 181 QTELIFQTMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLSCLMINDIDA 240
           Q EL+F+ MG+ P++MSAGELES  AGEP KLIR+RYR A+++I+ +G + CL IND+DA
Sbjct: 181 QCELVFRKMGINPIMMSAGELESGNAGEPAKLIRQRYREAAEIIR-KGNMCCLFINDLDA 240

Query: 241 GIGRF-GNTQVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIILTGNDFSTIY 300
           G GR  G TQ TVNNQ+   TLMN+ADNPT V +   + + +   R+PII+TGNDFST+Y
Sbjct: 241 GAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKQE-NARVPIIVTGNDFSTLY 300

Query: 301 APLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQALDFYGALRSR 360
           APLIRDGRMEKFYW P RED + +   ++  D +    V+ IV++FP Q++DF+GALR+R
Sbjct: 301 APLIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVPEEAVVKIVDSFPGQSIDFFGALRAR 360

Query: 361 TYDAAVSKWVDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQQLIM 420
            YD  V KWV    G+E +G+KLL  R     PTF  P+ T+E LLE G  LV+EQ+ + 
Sbjct: 361 VYDDEVRKWVSGT-GIELIGEKLLNSRDGP--PTFEQPKMTLEKLLEYGNMLVQEQENVK 417

Query: 421 ETKLSKEYMK 422
             +L++ Y+K
Sbjct: 421 RVQLAETYLK 417

BLAST of MC09g0399 vs. ExPASy Swiss-Prot
Match: Q40565 (Ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic OS=Nicotiana tabacum OX=4097 GN=RCA PE=2 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 8.8e-91
Identity = 200/443 (45.15%), Postives = 270/443 (60.95%), Query Frame = 0

Query: 1   MAISIS--GLSLPSLFQYKNSTIAREIRIRSFAIRSSKDVPGETSGGGDGEPSERLKRLS 60
           MA S+S  G +  +     NS     +   +F  ++ K V G+      G  S ++   S
Sbjct: 1   MATSVSTIGAANKAPLSLNNSVAGTSVPSTAFFGKTLKKVYGK------GVSSPKVTNRS 60

Query: 61  EQSSWEAKDSEGRDYLYR-------LGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIV 120
            + + E KD++ +   Y             D  +IA G   G++D LF        +   
Sbjct: 61  LRIAAEEKDADPKKQTYSDRWKGLVQDFSDDQQDIARG--KGMVDSLF-------QAPTG 120

Query: 121 FDYRHKVTRSFEYL-QG-----------DYYIAPVFMDKVVCHIVKNFIAHLLNIKVPLI 180
               H V +S+EY+ QG            +YIAP FMDK+V HI KNF+  L NIKVPLI
Sbjct: 121 TGTHHAVLQSYEYVSQGLRQYNMDNTLDGFYIAPSFMDKLVVHITKNFL-KLPNIKVPLI 180

Query: 181 LGIWGGKGQGKSFQTELIFQTMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQ 240
           LG+WGGKGQGKSFQ EL+F+ MG+ P++MSAGELES  AGEP KLIR+RYR A+++I+ +
Sbjct: 181 LGVWGGKGQGKSFQCELVFRKMGINPIMMSAGELESGNAGEPAKLIRQRYREAAEIIR-K 240

Query: 241 GKLSCLMINDIDAGIGRF-GNTQVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRI 300
           G + CL IND+DAG GR  G TQ TVNNQ+   TLMN+ADNPT V +   + + +   R+
Sbjct: 241 GNICCLFINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKQE-NARV 300

Query: 301 PIILTGNDFSTIYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFP 360
           PII+TGNDFST+YAPLIRDGRMEKFYW P RED + +   ++  D +    VI IV+TFP
Sbjct: 301 PIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVPEEAVIKIVDTFP 360

Query: 361 NQALDFYGALRSRTYDAAVSKWVDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLE 420
            Q++DF+GALR+R YD  V KWV    G+E +GDKLL     +  PTF  P+ T+E LLE
Sbjct: 361 GQSIDFFGALRARVYDDEVRKWVSGT-GIEAIGDKLL--NSFDGPPTFEQPKMTVEKLLE 420

Query: 421 AGYSLVKEQQLIMETKLSKEYMK 422
            G  LV+EQ+ +   +L++ Y+K
Sbjct: 421 YGNMLVQEQENVKRVQLAETYLK 422

BLAST of MC09g0399 vs. ExPASy Swiss-Prot
Match: Q40460 (Ribulose bisphosphate carboxylase/oxygenase activase 1, chloroplastic OS=Nicotiana tabacum OX=4097 PE=1 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 3.3e-90
Identity = 197/439 (44.87%), Postives = 267/439 (60.82%), Query Frame = 0

Query: 1   MAISIS--GLSLPSLFQYKNSTIAREIRIRSFAIRSSKDVPGETSGGGDGEP---SERLK 60
           MA S+S  G    +     NS     +   +F  ++ K V G+    G   P   ++ L+
Sbjct: 1   MATSVSTIGAVNKTPLSLNNSVAGTSVPSTAFFGKTLKKVYGK----GVSSPKVTNKSLR 60

Query: 61  RLSEQSSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYR 120
            ++EQ   + K     D    L  +  +    +    G++D LF        +       
Sbjct: 61  IVAEQIDVDPKKQTDSDRWKGLVQDFSDDQQDITRGKGMVDSLF-------QAPTGTGTH 120

Query: 121 HKVTRSFEYL-QG-----------DYYIAPVFMDKVVCHIVKNFIAHLLNIKVPLILGIW 180
           H V +S+EY+ QG            +YIAP FMDK+V HI KNF+  L NIKVPLILGIW
Sbjct: 121 HAVLQSYEYVSQGLRQYNLDNKLDGFYIAPAFMDKLVVHITKNFL-KLPNIKVPLILGIW 180

Query: 181 GGKGQGKSFQTELIFQTMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLS 240
           GGKGQGKSFQ EL+F+ MG+ P++MSAGELES  AGEP KLIR+RYR A+++I+ +G + 
Sbjct: 181 GGKGQGKSFQCELVFRKMGINPIMMSAGELESGNAGEPAKLIRQRYREAAEIIR-KGNMC 240

Query: 241 CLMINDIDAGIGRF-GNTQVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIIL 300
           CL IND+DAG GR  G TQ TVNNQ+   TLMN+ADNPT V +   + + +   R+PII+
Sbjct: 241 CLFINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKQE-NARVPIIV 300

Query: 301 TGNDFSTIYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQAL 360
           TGNDFST+YAPLIRDGRMEKFYW P RED + +   ++  D +    V+ IV+ FP Q++
Sbjct: 301 TGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCTGIFRTDNVPAEDVVKIVDNFPGQSI 360

Query: 361 DFYGALRSRTYDAAVSKWVDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYS 420
           DF+GALR+R YD  V KWV    G+EK+GDKLL     +  PTF  P+ T+E LLE G  
Sbjct: 361 DFFGALRARVYDDEVRKWVSGT-GIEKIGDKLL--NSFDGPPTFEQPKMTIEKLLEYGNM 420

Query: 421 LVKEQQLIMETKLSKEYMK 422
           LV+EQ+ +   +L+ +Y+K
Sbjct: 421 LVQEQENVKRVQLADKYLK 422

BLAST of MC09g0399 vs. ExPASy Swiss-Prot
Match: Q9ZT00 (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Zea mays OX=4577 GN=RCA1 PE=2 SV=3)

HSP 1 Score: 331.6 bits (849), Expect = 1.3e-89
Identity = 183/386 (47.41%), Postives = 251/386 (65.03%), Query Frame = 0

Query: 42  TSGGGDGEPSERLKRLSEQSSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAG 101
           T  G     + R K ++ +   E K ++  D    L  +  +    +    G++D+LF  
Sbjct: 36  TYHGKSSSSNSRFKAMAAKEVDETKQTD-EDRWKGLAYDISDDQQDITRGKGLVDNLFQA 95

Query: 102 NFLGRDSDIV----FDYRHKVTRS--FEYLQGDYYIAPVFMDKVVCHIVKNFIAHLLNIK 161
             +G  + +     +DY  +  +S  F+ +   +YIA  FMDK+V H+ KNF+  L NIK
Sbjct: 96  P-MGDGTHVAVLSSYDYISQGQKSYNFDNMMDGFYIAKGFMDKLVVHLSKNFMT-LPNIK 155

Query: 162 VPLILGIWGGKGQGKSFQTELIFQTMGVEPVIMSAGELESERAGEPGKLIRERYRTASQV 221
           VPLILGIWGGKGQGKSFQ EL+F  MG+ P++MSAGELES  AGEP KLIR+RYR AS +
Sbjct: 156 VPLILGIWGGKGQGKSFQCELVFAKMGITPIMMSAGELESGNAGEPAKLIRQRYREASDL 215

Query: 222 IQNQGKLSCLMINDIDAGIGRF-GNTQVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDI 281
           I+ +GK+SCL IND+DAG GR  G TQ TVNNQ+   TLMN+ADNPT V +   + + D 
Sbjct: 216 IK-KGKMSCLFINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKED- 275

Query: 282 TNRIPIILTGNDFSTIYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIV 341
             R+PII+TGNDFST+YAPLIRDGRMEKFYW P RED + +   ++  DG+    V+ +V
Sbjct: 276 NPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFRTDGVDEEHVVQLV 335

Query: 342 NTFPNQALDFYGALRSRTYDAAVSKWVDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTME 401
           +TFP Q++DF+GALR+R YD  V +WV +  GVE +  KL+  +  E  PTF  P+ T+E
Sbjct: 336 DTFPGQSIDFFGALRARVYDDEVRRWVSET-GVENIARKLVNSK--EGPPTFEQPKITIE 395

Query: 402 ALLEAGYSLVKEQQLIMETKLSKEYM 421
            LLE G+ LV EQ+ +   +L+ +Y+
Sbjct: 396 KLLEYGHMLVAEQENVKRVQLADKYL 413

BLAST of MC09g0399 vs. NCBI nr
Match: XP_022154094.1 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic isoform X1 [Momordica charantia] >XP_022154101.1 ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic isoform X1 [Momordica charantia] >XP_022154111.1 ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 842 bits (2176), Expect = 4.92e-308
Identity = 425/425 (100.00%), Postives = 425/425 (100.00%), Query Frame = 0

Query: 1   MAISISGLSLPSLFQYKNSTIAREIRIRSFAIRSSKDVPGETSGGGDGEPSERLKRLSEQ 60
           MAISISGLSLPSLFQYKNSTIAREIRIRSFAIRSSKDVPGETSGGGDGEPSERLKRLSEQ
Sbjct: 1   MAISISGLSLPSLFQYKNSTIAREIRIRSFAIRSSKDVPGETSGGGDGEPSERLKRLSEQ 60

Query: 61  SSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRHKVTR 120
           SSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRHKVTR
Sbjct: 61  SSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRHKVTR 120

Query: 121 SFEYLQGDYYIAPVFMDKVVCHIVKNFIAHLLNIKVPLILGIWGGKGQGKSFQTELIFQT 180
           SFEYLQGDYYIAPVFMDKVVCHIVKNFIAHLLNIKVPLILGIWGGKGQGKSFQTELIFQT
Sbjct: 121 SFEYLQGDYYIAPVFMDKVVCHIVKNFIAHLLNIKVPLILGIWGGKGQGKSFQTELIFQT 180

Query: 181 MGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLSCLMINDIDAGIGRFGNT 240
           MGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLSCLMINDIDAGIGRFGNT
Sbjct: 181 MGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLSCLMINDIDAGIGRFGNT 240

Query: 241 QVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIILTGNDFSTIYAPLIRDGRM 300
           QVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIILTGNDFSTIYAPLIRDGRM
Sbjct: 241 QVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIILTGNDFSTIYAPLIRDGRM 300

Query: 301 EKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQALDFYGALRSRTYDAAVSKW 360
           EKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQALDFYGALRSRTYDAAVSKW
Sbjct: 301 EKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQALDFYGALRSRTYDAAVSKW 360

Query: 361 VDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQQLIMETKLSKEYM 420
           VDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQQLIMETKLSKEYM
Sbjct: 361 VDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQQLIMETKLSKEYM 420

Query: 421 KNMDD 425
           KNMDD
Sbjct: 421 KNMDD 425

BLAST of MC09g0399 vs. NCBI nr
Match: XP_022960536.1 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic [Cucurbita moschata])

HSP 1 Score: 775 bits (2002), Expect = 1.56e-281
Identity = 386/425 (90.82%), Postives = 407/425 (95.76%), Query Frame = 0

Query: 1   MAISISGLSLPSLFQYKNSTIAREIRIRSFAIRSSKDVPGETSGGGDGEPSERLKRLSEQ 60
           MAI+ISG+SLPSLF YKNS IAR+ RIRSF+IRS KDV GE  GGG GEPS++ KRLSE+
Sbjct: 1   MAIAISGVSLPSLFHYKNSIIARKSRIRSFSIRSCKDVSGEGDGGG-GEPSQKPKRLSEK 60

Query: 61  SSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRHKVTR 120
           SSWEAKDSEG+DYLYRLG+EADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYR KVTR
Sbjct: 61  SSWEAKDSEGKDYLYRLGSEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRQKVTR 120

Query: 121 SFEYLQGDYYIAPVFMDKVVCHIVKNFIAHLLNIKVPLILGIWGGKGQGKSFQTELIFQT 180
           SFE+LQGDYYIAPVFMDKVVCHIVKNFI HLLNIKVPLILGIWGGKGQGKSFQTELIFQ 
Sbjct: 121 SFEHLQGDYYIAPVFMDKVVCHIVKNFIVHLLNIKVPLILGIWGGKGQGKSFQTELIFQA 180

Query: 181 MGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLSCLMINDIDAGIGRFGNT 240
           MGVEPVIMSAGELESERAGEPGKLIRERYRTASQV+QNQGKLSCLMINDIDAGIGRF NT
Sbjct: 181 MGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQGKLSCLMINDIDAGIGRFENT 240

Query: 241 QVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIILTGNDFSTIYAPLIRDGRM 300
           QVTVNNQI AGTLMNLADNP RVSIGQDWRE+DITNRIPIILTGNDFSTIYAPLIRDGRM
Sbjct: 241 QVTVNNQIVAGTLMNLADNPNRVSIGQDWREADITNRIPIILTGNDFSTIYAPLIRDGRM 300

Query: 301 EKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQALDFYGALRSRTYDAAVSKW 360
           EKFYWQPNREDIVNIVHRMYEKDGISR+QVIDIVNTFPNQALDFYGALRSRTYD ++SKW
Sbjct: 301 EKFYWQPNREDIVNIVHRMYEKDGISRAQVIDIVNTFPNQALDFYGALRSRTYDTSISKW 360

Query: 361 VDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQQLIMETKLSKEYM 420
           VDDIGG+EKLG+KLLKRRKNE+LPTFTPPEQTM+ LLEAGYSL+KEQQ +METKLSKEYM
Sbjct: 361 VDDIGGMEKLGEKLLKRRKNEELPTFTPPEQTMDTLLEAGYSLIKEQQFVMETKLSKEYM 420

Query: 421 KNMDD 425
           KNMDD
Sbjct: 421 KNMDD 424

BLAST of MC09g0399 vs. NCBI nr
Match: XP_023515166.1 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 773 bits (1997), Expect = 8.69e-281
Identity = 386/425 (90.82%), Postives = 406/425 (95.53%), Query Frame = 0

Query: 1   MAISISGLSLPSLFQYKNSTIAREIRIRSFAIRSSKDVPGETSGGGDGEPSERLKRLSEQ 60
           MAI+ISG+SLPSLF YKNS IAR+ RIRSF+IRS KDV GE  GGG  EPS++ KRLSE+
Sbjct: 1   MAIAISGVSLPSLFHYKNSIIARKSRIRSFSIRSCKDVSGEGDGGG--EPSQKPKRLSEK 60

Query: 61  SSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRHKVTR 120
           SSWEAKDSEG+DYLYRLG+EADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYR KVTR
Sbjct: 61  SSWEAKDSEGKDYLYRLGSEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRQKVTR 120

Query: 121 SFEYLQGDYYIAPVFMDKVVCHIVKNFIAHLLNIKVPLILGIWGGKGQGKSFQTELIFQT 180
           SFE+LQGDYYIAPVFMDKVVCHIVKNFI HLLNIKVPLILGIWGGKGQGKSFQTELIFQ 
Sbjct: 121 SFEHLQGDYYIAPVFMDKVVCHIVKNFIVHLLNIKVPLILGIWGGKGQGKSFQTELIFQA 180

Query: 181 MGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLSCLMINDIDAGIGRFGNT 240
           MGVEPVIMSAGELESERAGEPGKLIRERYRTASQV+QNQGKLSCLMINDIDAGIGRF NT
Sbjct: 181 MGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQGKLSCLMINDIDAGIGRFENT 240

Query: 241 QVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIILTGNDFSTIYAPLIRDGRM 300
           QVTVNNQI AGTLMNLADNP RVSIGQDWRE+DITNRIPIILTGNDFSTIYAPLIRDGRM
Sbjct: 241 QVTVNNQIVAGTLMNLADNPNRVSIGQDWREADITNRIPIILTGNDFSTIYAPLIRDGRM 300

Query: 301 EKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQALDFYGALRSRTYDAAVSKW 360
           EKFYWQPNREDIVNIVHRMYEKDGISR+QVIDIVNTFPNQALDFYGALRSRTYD ++SKW
Sbjct: 301 EKFYWQPNREDIVNIVHRMYEKDGISRAQVIDIVNTFPNQALDFYGALRSRTYDTSISKW 360

Query: 361 VDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQQLIMETKLSKEYM 420
           VDDIGG+EKLG+KLLKRRKNE+LPTFTPPEQTM+ LLEAGYSLVKEQQ +METKLSKEYM
Sbjct: 361 VDDIGGMEKLGEKLLKRRKNEELPTFTPPEQTMDTLLEAGYSLVKEQQFVMETKLSKEYM 420

Query: 421 KNMDD 425
           KNMDD
Sbjct: 421 KNMDD 423

BLAST of MC09g0399 vs. NCBI nr
Match: XP_023004209.1 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 773 bits (1997), Expect = 8.69e-281
Identity = 386/425 (90.82%), Postives = 405/425 (95.29%), Query Frame = 0

Query: 1   MAISISGLSLPSLFQYKNSTIAREIRIRSFAIRSSKDVPGETSGGGDGEPSERLKRLSEQ 60
           MAI+IS +SLPSLFQYKN  IAR+ RIRSF+IRS KDV GE  GGG  EP ++ KRLSE+
Sbjct: 1   MAIAISAVSLPSLFQYKNLIIARKSRIRSFSIRSCKDVSGEGDGGG--EPLQKPKRLSEK 60

Query: 61  SSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRHKVTR 120
           SSWEAKDSEG+DYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYR KVTR
Sbjct: 61  SSWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRQKVTR 120

Query: 121 SFEYLQGDYYIAPVFMDKVVCHIVKNFIAHLLNIKVPLILGIWGGKGQGKSFQTELIFQT 180
           SFE+LQGDYYIAPVFMDKVVCHIVKNFI HLLNIKVPLILGIWGGKGQGKSFQTELIFQ 
Sbjct: 121 SFEHLQGDYYIAPVFMDKVVCHIVKNFIVHLLNIKVPLILGIWGGKGQGKSFQTELIFQA 180

Query: 181 MGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLSCLMINDIDAGIGRFGNT 240
           MGVEPVIMSAGELESERAGEPGKLIRERYRTASQV+QNQGKLSCLMINDIDAGIGRFGNT
Sbjct: 181 MGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQGKLSCLMINDIDAGIGRFGNT 240

Query: 241 QVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIILTGNDFSTIYAPLIRDGRM 300
           QVTVNNQI AGTLMNLADNP RVSIGQDWRE+DITNRIPIILTGNDFSTIYAPLIRDGRM
Sbjct: 241 QVTVNNQIVAGTLMNLADNPNRVSIGQDWREADITNRIPIILTGNDFSTIYAPLIRDGRM 300

Query: 301 EKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQALDFYGALRSRTYDAAVSKW 360
           EKFYWQPNREDIVNIVHRMYEKDGISR+QVIDIVNTFPNQALDFYGALRSRTYD ++SKW
Sbjct: 301 EKFYWQPNREDIVNIVHRMYEKDGISRAQVIDIVNTFPNQALDFYGALRSRTYDTSISKW 360

Query: 361 VDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQQLIMETKLSKEYM 420
           VDDIGG+EKLG+KLLKRRKNE+LPTFTPPEQTM+ LLEAGYSLVKEQQ +METKLSKEYM
Sbjct: 361 VDDIGGMEKLGEKLLKRRKNEELPTFTPPEQTMDTLLEAGYSLVKEQQFVMETKLSKEYM 420

Query: 421 KNMDD 425
           KNMDD
Sbjct: 421 KNMDD 423

BLAST of MC09g0399 vs. NCBI nr
Match: XP_038900248.1 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 768 bits (1983), Expect = 1.37e-278
Identity = 384/427 (89.93%), Postives = 407/427 (95.32%), Query Frame = 0

Query: 1   MAISISGLSLPSLFQYKNSTIAREIRIRSFAIRSSKDVPGET-SGGGDG-EPSERLKRLS 60
           MA SISGLSLPS+FQY++S +AR+ R RSF+IRSS DV GE  SGGGDG EPS R KRLS
Sbjct: 1   MAFSISGLSLPSVFQYESSILARKPRFRSFSIRSSNDVSGEVGSGGGDGSEPSNRPKRLS 60

Query: 61  EQSSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRHKV 120
           EQS+WEAKDSEG+DYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLG+DSDIVFDYR KV
Sbjct: 61  EQSTWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGKDSDIVFDYRQKV 120

Query: 121 TRSFEYLQGDYYIAPVFMDKVVCHIVKNFIAHLLNIKVPLILGIWGGKGQGKSFQTELIF 180
           TRSF +LQGDYYIAPVFMDKVVCHIVKNF+ HLLN+KVPLILG+WGGKGQGKSFQTELIF
Sbjct: 121 TRSFGHLQGDYYIAPVFMDKVVCHIVKNFMVHLLNVKVPLILGVWGGKGQGKSFQTELIF 180

Query: 181 QTMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLSCLMINDIDAGIGRFG 240
           Q MGVEPVIMSAGELESERAGEPGKLIRERYRTASQV+QNQGKLSCLMINDIDAGIGRFG
Sbjct: 181 QAMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQGKLSCLMINDIDAGIGRFG 240

Query: 241 NTQVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIILTGNDFSTIYAPLIRDG 300
           +TQVTVNNQI +GTLMNLADNPTRVS+GQDWRE+DI NRIPIILTGNDFSTIYAPLIRDG
Sbjct: 241 STQVTVNNQIVSGTLMNLADNPTRVSVGQDWREADILNRIPIILTGNDFSTIYAPLIRDG 300

Query: 301 RMEKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQALDFYGALRSRTYDAAVS 360
           RMEKFYWQPNREDIVNIVHRMYEKDGISR+QV DIVN FPNQALDFYGALRSRTYDAA+S
Sbjct: 301 RMEKFYWQPNREDIVNIVHRMYEKDGISRAQVADIVNIFPNQALDFYGALRSRTYDAAIS 360

Query: 361 KWVDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQQLIMETKLSKE 420
           KWVDDIGGVEKLG+KLLKRRKNEKLPTF PPEQT+EALLEAGYSLVKEQ+LIMETKLSKE
Sbjct: 361 KWVDDIGGVEKLGEKLLKRRKNEKLPTFVPPEQTLEALLEAGYSLVKEQKLIMETKLSKE 420

Query: 421 YMKNMDD 425
           YMKNMDD
Sbjct: 421 YMKNMDD 427

BLAST of MC09g0399 vs. ExPASy TrEMBL
Match: A0A6J1DL15 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111021429 PE=4 SV=1)

HSP 1 Score: 842 bits (2176), Expect = 2.38e-308
Identity = 425/425 (100.00%), Postives = 425/425 (100.00%), Query Frame = 0

Query: 1   MAISISGLSLPSLFQYKNSTIAREIRIRSFAIRSSKDVPGETSGGGDGEPSERLKRLSEQ 60
           MAISISGLSLPSLFQYKNSTIAREIRIRSFAIRSSKDVPGETSGGGDGEPSERLKRLSEQ
Sbjct: 1   MAISISGLSLPSLFQYKNSTIAREIRIRSFAIRSSKDVPGETSGGGDGEPSERLKRLSEQ 60

Query: 61  SSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRHKVTR 120
           SSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRHKVTR
Sbjct: 61  SSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRHKVTR 120

Query: 121 SFEYLQGDYYIAPVFMDKVVCHIVKNFIAHLLNIKVPLILGIWGGKGQGKSFQTELIFQT 180
           SFEYLQGDYYIAPVFMDKVVCHIVKNFIAHLLNIKVPLILGIWGGKGQGKSFQTELIFQT
Sbjct: 121 SFEYLQGDYYIAPVFMDKVVCHIVKNFIAHLLNIKVPLILGIWGGKGQGKSFQTELIFQT 180

Query: 181 MGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLSCLMINDIDAGIGRFGNT 240
           MGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLSCLMINDIDAGIGRFGNT
Sbjct: 181 MGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLSCLMINDIDAGIGRFGNT 240

Query: 241 QVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIILTGNDFSTIYAPLIRDGRM 300
           QVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIILTGNDFSTIYAPLIRDGRM
Sbjct: 241 QVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIILTGNDFSTIYAPLIRDGRM 300

Query: 301 EKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQALDFYGALRSRTYDAAVSKW 360
           EKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQALDFYGALRSRTYDAAVSKW
Sbjct: 301 EKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQALDFYGALRSRTYDAAVSKW 360

Query: 361 VDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQQLIMETKLSKEYM 420
           VDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQQLIMETKLSKEYM
Sbjct: 361 VDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQQLIMETKLSKEYM 420

Query: 421 KNMDD 425
           KNMDD
Sbjct: 421 KNMDD 425

BLAST of MC09g0399 vs. ExPASy TrEMBL
Match: A0A6J1H7W0 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111461246 PE=4 SV=1)

HSP 1 Score: 775 bits (2002), Expect = 7.56e-282
Identity = 386/425 (90.82%), Postives = 407/425 (95.76%), Query Frame = 0

Query: 1   MAISISGLSLPSLFQYKNSTIAREIRIRSFAIRSSKDVPGETSGGGDGEPSERLKRLSEQ 60
           MAI+ISG+SLPSLF YKNS IAR+ RIRSF+IRS KDV GE  GGG GEPS++ KRLSE+
Sbjct: 1   MAIAISGVSLPSLFHYKNSIIARKSRIRSFSIRSCKDVSGEGDGGG-GEPSQKPKRLSEK 60

Query: 61  SSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRHKVTR 120
           SSWEAKDSEG+DYLYRLG+EADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYR KVTR
Sbjct: 61  SSWEAKDSEGKDYLYRLGSEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRQKVTR 120

Query: 121 SFEYLQGDYYIAPVFMDKVVCHIVKNFIAHLLNIKVPLILGIWGGKGQGKSFQTELIFQT 180
           SFE+LQGDYYIAPVFMDKVVCHIVKNFI HLLNIKVPLILGIWGGKGQGKSFQTELIFQ 
Sbjct: 121 SFEHLQGDYYIAPVFMDKVVCHIVKNFIVHLLNIKVPLILGIWGGKGQGKSFQTELIFQA 180

Query: 181 MGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLSCLMINDIDAGIGRFGNT 240
           MGVEPVIMSAGELESERAGEPGKLIRERYRTASQV+QNQGKLSCLMINDIDAGIGRF NT
Sbjct: 181 MGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQGKLSCLMINDIDAGIGRFENT 240

Query: 241 QVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIILTGNDFSTIYAPLIRDGRM 300
           QVTVNNQI AGTLMNLADNP RVSIGQDWRE+DITNRIPIILTGNDFSTIYAPLIRDGRM
Sbjct: 241 QVTVNNQIVAGTLMNLADNPNRVSIGQDWREADITNRIPIILTGNDFSTIYAPLIRDGRM 300

Query: 301 EKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQALDFYGALRSRTYDAAVSKW 360
           EKFYWQPNREDIVNIVHRMYEKDGISR+QVIDIVNTFPNQALDFYGALRSRTYD ++SKW
Sbjct: 301 EKFYWQPNREDIVNIVHRMYEKDGISRAQVIDIVNTFPNQALDFYGALRSRTYDTSISKW 360

Query: 361 VDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQQLIMETKLSKEYM 420
           VDDIGG+EKLG+KLLKRRKNE+LPTFTPPEQTM+ LLEAGYSL+KEQQ +METKLSKEYM
Sbjct: 361 VDDIGGMEKLGEKLLKRRKNEELPTFTPPEQTMDTLLEAGYSLIKEQQFVMETKLSKEYM 420

Query: 421 KNMDD 425
           KNMDD
Sbjct: 421 KNMDD 424

BLAST of MC09g0399 vs. ExPASy TrEMBL
Match: A0A6J1KVM6 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111497605 PE=4 SV=1)

HSP 1 Score: 773 bits (1997), Expect = 4.21e-281
Identity = 386/425 (90.82%), Postives = 405/425 (95.29%), Query Frame = 0

Query: 1   MAISISGLSLPSLFQYKNSTIAREIRIRSFAIRSSKDVPGETSGGGDGEPSERLKRLSEQ 60
           MAI+IS +SLPSLFQYKN  IAR+ RIRSF+IRS KDV GE  GGG  EP ++ KRLSE+
Sbjct: 1   MAIAISAVSLPSLFQYKNLIIARKSRIRSFSIRSCKDVSGEGDGGG--EPLQKPKRLSEK 60

Query: 61  SSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRHKVTR 120
           SSWEAKDSEG+DYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYR KVTR
Sbjct: 61  SSWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRQKVTR 120

Query: 121 SFEYLQGDYYIAPVFMDKVVCHIVKNFIAHLLNIKVPLILGIWGGKGQGKSFQTELIFQT 180
           SFE+LQGDYYIAPVFMDKVVCHIVKNFI HLLNIKVPLILGIWGGKGQGKSFQTELIFQ 
Sbjct: 121 SFEHLQGDYYIAPVFMDKVVCHIVKNFIVHLLNIKVPLILGIWGGKGQGKSFQTELIFQA 180

Query: 181 MGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLSCLMINDIDAGIGRFGNT 240
           MGVEPVIMSAGELESERAGEPGKLIRERYRTASQV+QNQGKLSCLMINDIDAGIGRFGNT
Sbjct: 181 MGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQGKLSCLMINDIDAGIGRFGNT 240

Query: 241 QVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIILTGNDFSTIYAPLIRDGRM 300
           QVTVNNQI AGTLMNLADNP RVSIGQDWRE+DITNRIPIILTGNDFSTIYAPLIRDGRM
Sbjct: 241 QVTVNNQIVAGTLMNLADNPNRVSIGQDWREADITNRIPIILTGNDFSTIYAPLIRDGRM 300

Query: 301 EKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQALDFYGALRSRTYDAAVSKW 360
           EKFYWQPNREDIVNIVHRMYEKDGISR+QVIDIVNTFPNQALDFYGALRSRTYD ++SKW
Sbjct: 301 EKFYWQPNREDIVNIVHRMYEKDGISRAQVIDIVNTFPNQALDFYGALRSRTYDTSISKW 360

Query: 361 VDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQQLIMETKLSKEYM 420
           VDDIGG+EKLG+KLLKRRKNE+LPTFTPPEQTM+ LLEAGYSLVKEQQ +METKLSKEYM
Sbjct: 361 VDDIGGMEKLGEKLLKRRKNEELPTFTPPEQTMDTLLEAGYSLVKEQQFVMETKLSKEYM 420

Query: 421 KNMDD 425
           KNMDD
Sbjct: 421 KNMDD 423

BLAST of MC09g0399 vs. ExPASy TrEMBL
Match: A0A5A7UYP6 (Ribulose bisphosphate carboxylase/oxygenase activase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G001550 PE=4 SV=1)

HSP 1 Score: 763 bits (1969), Expect = 8.70e-277
Identity = 376/426 (88.26%), Postives = 405/426 (95.07%), Query Frame = 0

Query: 1   MAISISGLSLPSLFQYKNSTIAREIRIRSFAIRSSKDVPGETSGGGDG-EPSERLKRLSE 60
           MA SISGLSLPS+F+YK+S +AR+ R  SF++RSS DV  E   GGDG + S+R KRLSE
Sbjct: 1   MAFSISGLSLPSVFRYKSSILARKPRFHSFSVRSSNDVSSEVGSGGDGSDSSKRPKRLSE 60

Query: 61  QSSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRHKVT 120
           QS+WEAKDSEG+DYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLG+DSDIVFDYR KVT
Sbjct: 61  QSTWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGKDSDIVFDYRQKVT 120

Query: 121 RSFEYLQGDYYIAPVFMDKVVCHIVKNFIAHLLNIKVPLILGIWGGKGQGKSFQTELIFQ 180
           RSFE+LQGDYYIAP FMDKVVCHIVKNFI HLLN+KVPLILG+WGGKGQGKSFQTELIFQ
Sbjct: 121 RSFEHLQGDYYIAPTFMDKVVCHIVKNFIVHLLNVKVPLILGVWGGKGQGKSFQTELIFQ 180

Query: 181 TMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLSCLMINDIDAGIGRFGN 240
            MGVEPVIMSAGELESERAGEPGKLIRERYRTASQV+QNQGK+SCLMINDIDAGIGRFG 
Sbjct: 181 MMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQGKMSCLMINDIDAGIGRFGQ 240

Query: 241 TQVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIILTGNDFSTIYAPLIRDGR 300
           TQVTVNNQI +GTLMNLADNPTRVS+GQDWRE+DI +RIPIILTGNDFSTIYAPLIRDGR
Sbjct: 241 TQVTVNNQIVSGTLMNLADNPTRVSVGQDWREADILHRIPIILTGNDFSTIYAPLIRDGR 300

Query: 301 MEKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQALDFYGALRSRTYDAAVSK 360
           MEKFYWQPNREDIVNIVHRMYEKDGISR+QV+DIVNTFPNQALDFYGALRSRTYD+A+SK
Sbjct: 301 MEKFYWQPNREDIVNIVHRMYEKDGISRAQVVDIVNTFPNQALDFYGALRSRTYDSAISK 360

Query: 361 WVDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQQLIMETKLSKEY 420
           WVDDIGGVEKLG+KLL+RRKNEKLPTFTPPEQT+EALL+AGYSLVKEQQLIMETKLSKEY
Sbjct: 361 WVDDIGGVEKLGEKLLRRRKNEKLPTFTPPEQTLEALLKAGYSLVKEQQLIMETKLSKEY 420

Query: 421 MKNMDD 425
           MKNMDD
Sbjct: 421 MKNMDD 426

BLAST of MC09g0399 vs. ExPASy TrEMBL
Match: A0A1S3CFE2 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103499863 PE=4 SV=1)

HSP 1 Score: 763 bits (1969), Expect = 8.70e-277
Identity = 376/426 (88.26%), Postives = 405/426 (95.07%), Query Frame = 0

Query: 1   MAISISGLSLPSLFQYKNSTIAREIRIRSFAIRSSKDVPGETSGGGDG-EPSERLKRLSE 60
           MA SISGLSLPS+F+YK+S +AR+ R  SF++RSS DV  E   GGDG + S+R KRLSE
Sbjct: 1   MAFSISGLSLPSVFRYKSSILARKPRFHSFSVRSSNDVSSEVGSGGDGSDSSKRPKRLSE 60

Query: 61  QSSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRHKVT 120
           QS+WEAKDSEG+DYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLG+DSDIVFDYR KVT
Sbjct: 61  QSTWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGKDSDIVFDYRQKVT 120

Query: 121 RSFEYLQGDYYIAPVFMDKVVCHIVKNFIAHLLNIKVPLILGIWGGKGQGKSFQTELIFQ 180
           RSFE+LQGDYYIAP FMDKVVCHIVKNFI HLLN+KVPLILG+WGGKGQGKSFQTELIFQ
Sbjct: 121 RSFEHLQGDYYIAPTFMDKVVCHIVKNFIVHLLNVKVPLILGVWGGKGQGKSFQTELIFQ 180

Query: 181 TMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLSCLMINDIDAGIGRFGN 240
            MGVEPVIMSAGELESERAGEPGKLIRERYRTASQV+QNQGK+SCLMINDIDAGIGRFG 
Sbjct: 181 MMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQGKMSCLMINDIDAGIGRFGQ 240

Query: 241 TQVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIILTGNDFSTIYAPLIRDGR 300
           TQVTVNNQI +GTLMNLADNPTRVS+GQDWRE+DI +RIPIILTGNDFSTIYAPLIRDGR
Sbjct: 241 TQVTVNNQIVSGTLMNLADNPTRVSVGQDWREADILHRIPIILTGNDFSTIYAPLIRDGR 300

Query: 301 MEKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQALDFYGALRSRTYDAAVSK 360
           MEKFYWQPNREDIVNIVHRMYEKDGISR+QV+DIVNTFPNQALDFYGALRSRTYD+A+SK
Sbjct: 301 MEKFYWQPNREDIVNIVHRMYEKDGISRAQVVDIVNTFPNQALDFYGALRSRTYDSAISK 360

Query: 361 WVDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQQLIMETKLSKEY 420
           WVDDIGGVEKLG+KLL+RRKNEKLPTFTPPEQT+EALL+AGYSLVKEQQLIMETKLSKEY
Sbjct: 361 WVDDIGGVEKLGEKLLRRRKNEKLPTFTPPEQTLEALLKAGYSLVKEQQLIMETKLSKEY 420

Query: 421 MKNMDD 425
           MKNMDD
Sbjct: 421 MKNMDD 426

BLAST of MC09g0399 vs. TAIR 10
Match: AT1G73110.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 622.5 bits (1604), Expect = 2.6e-178
Identity = 302/408 (74.02%), Postives = 351/408 (86.03%), Query Frame = 0

Query: 18  NSTIAREIRIRSFAIRSSKDVPGETSGGGDGEPSERLKRLSEQSSWEAKDSEGRDYLYRL 77
           N+T+    ++ S          G      DG    R K+LSEQSSWE KDSEG+DYLYRL
Sbjct: 26  NATLINTRKLSSIVCSKPSSNDGGKVANDDGGAKPR-KKLSEQSSWEVKDSEGKDYLYRL 85

Query: 78  GNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRHKVTRSFEYLQGDYYIAPVFMD 137
           G E+DN+NIAVGAR+G+IDD+F G+FLG+DSDIVFDYR K TRSFE+LQGDYYIAP F+D
Sbjct: 86  GAESDNVNIAVGARSGMIDDVFIGDFLGKDSDIVFDYRQKATRSFEHLQGDYYIAPSFLD 145

Query: 138 KVVCHIVKNFIAHLLNIKVPLILGIWGGKGQGKSFQTELIFQTMGVEPVIMSAGELESER 197
           KV  HIVKN++A  LNIK+PLILGIWGGKGQGK+FQTELIF+TMGVEPVIMSAGELES+R
Sbjct: 146 KVAVHIVKNYLAPSLNIKIPLILGIWGGKGQGKTFQTELIFKTMGVEPVIMSAGELESDR 205

Query: 198 AGEPGKLIRERYRTASQVIQNQGKLSCLMINDIDAGIGRFGNTQVTVNNQIAAGTLMNLA 257
           AGEPG+LIR+RYRTASQVIQNQGK+S LMINDIDAG+GRFG TQ+TVNNQI  GTLMNLA
Sbjct: 206 AGEPGRLIRDRYRTASQVIQNQGKMSVLMINDIDAGLGRFGETQMTVNNQIVVGTLMNLA 265

Query: 258 DNPTRVSIGQDWRESDITNRIPIILTGNDFSTIYAPLIRDGRMEKFYWQPNREDIVNIVH 317
           DNPTRVS+GQ+WRE+D+ NR+P+I+TGNDFST+YAPLIR+GRMEKFYWQP REDIVNIV 
Sbjct: 266 DNPTRVSVGQEWREADMVNRVPLIVTGNDFSTLYAPLIREGRMEKFYWQPTREDIVNIVS 325

Query: 318 RMYEKDGISRSQVIDIVNTFPNQALDFYGALRSRTYDAAVSKWVDDIGGVEKLGDKLLKR 377
           RMYEKDGISR  VI IV+ FPNQALDFYGALRSRTYD ++ KWVD+ GG+E LG  LL+R
Sbjct: 326 RMYEKDGISRKDVISIVDKFPNQALDFYGALRSRTYDRSILKWVDEAGGMETLGKVLLRR 385

Query: 378 RKNEKLPTFTPPEQTMEALLEAGYSLVKEQQLIMETKLSKEYMKNMDD 426
           +K +++P FT PEQT+EALLE+GYSL+ EQ+LIMETKLSKEYMKNMDD
Sbjct: 386 KKTQEVPQFTAPEQTVEALLESGYSLINEQKLIMETKLSKEYMKNMDD 432

BLAST of MC09g0399 vs. TAIR 10
Match: AT2G39730.1 (rubisco activase )

HSP 1 Score: 323.2 bits (827), Expect = 3.2e-88
Identity = 168/318 (52.83%), Postives = 217/318 (68.24%), Query Frame = 0

Query: 116 HKVTRSFEY------------LQGDYYIAPVFMDKVVCHIVKNFIAHLLNIKVPLILGIW 175
           H V  S+EY            +   +YIAP FMDK+V HI KNF+  L NIKVPLILGIW
Sbjct: 106 HAVLSSYEYVSQGLRQYNLDNMMDGFYIAPAFMDKLVVHITKNFLT-LPNIKVPLILGIW 165

Query: 176 GGKGQGKSFQTELIFQTMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLS 235
           GGKGQGKSFQ EL+   MG+ P++MSAGELES  AGEP KLIR+RYR A+ +I+ +GK+ 
Sbjct: 166 GGKGQGKSFQCELVMAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIK-KGKMC 225

Query: 236 CLMINDIDAGIGRF-GNTQVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIIL 295
           CL IND+DAG GR  G TQ TVNNQ+   TLMN+ADNPT V +   + + +   R+PII 
Sbjct: 226 CLFINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEE-NARVPIIC 285

Query: 296 TGNDFSTIYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQAL 355
           TGNDFST+YAPLIRDGRMEKFYW P RED + +   ++  D I    ++ +V+ FP Q++
Sbjct: 286 TGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFRTDKIKDEDIVTLVDQFPGQSI 345

Query: 356 DFYGALRSRTYDAAVSKWVDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYS 415
           DF+GALR+R YD  V K+V+ + GVEK+G +L+  R  E  P F  PE T E L+E G  
Sbjct: 346 DFFGALRARVYDDEVRKFVESL-GVEKIGKRLVNSR--EGPPVFEQPEMTYEKLMEYGNM 405

Query: 416 LVKEQQLIMETKLSKEYM 421
           LV EQ+ +   +L++ Y+
Sbjct: 406 LVMEQENVKRVQLAETYL 417

BLAST of MC09g0399 vs. TAIR 10
Match: AT2G39730.2 (rubisco activase )

HSP 1 Score: 323.2 bits (827), Expect = 3.2e-88
Identity = 168/318 (52.83%), Postives = 217/318 (68.24%), Query Frame = 0

Query: 116 HKVTRSFEY------------LQGDYYIAPVFMDKVVCHIVKNFIAHLLNIKVPLILGIW 175
           H V  S+EY            +   +YIAP FMDK+V HI KNF+  L NIKVPLILGIW
Sbjct: 106 HAVLSSYEYVSQGLRQYNLDNMMDGFYIAPAFMDKLVVHITKNFLT-LPNIKVPLILGIW 165

Query: 176 GGKGQGKSFQTELIFQTMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLS 235
           GGKGQGKSFQ EL+   MG+ P++MSAGELES  AGEP KLIR+RYR A+ +I+ +GK+ 
Sbjct: 166 GGKGQGKSFQCELVMAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIK-KGKMC 225

Query: 236 CLMINDIDAGIGRF-GNTQVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIIL 295
           CL IND+DAG GR  G TQ TVNNQ+   TLMN+ADNPT V +   + + +   R+PII 
Sbjct: 226 CLFINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEE-NARVPIIC 285

Query: 296 TGNDFSTIYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQAL 355
           TGNDFST+YAPLIRDGRMEKFYW P RED + +   ++  D I    ++ +V+ FP Q++
Sbjct: 286 TGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFRTDKIKDEDIVTLVDQFPGQSI 345

Query: 356 DFYGALRSRTYDAAVSKWVDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYS 415
           DF+GALR+R YD  V K+V+ + GVEK+G +L+  R  E  P F  PE T E L+E G  
Sbjct: 346 DFFGALRARVYDDEVRKFVESL-GVEKIGKRLVNSR--EGPPVFEQPEMTYEKLMEYGNM 405

Query: 416 LVKEQQLIMETKLSKEYM 421
           LV EQ+ +   +L++ Y+
Sbjct: 406 LVMEQENVKRVQLAETYL 417

BLAST of MC09g0399 vs. TAIR 10
Match: AT2G39730.3 (rubisco activase )

HSP 1 Score: 323.2 bits (827), Expect = 3.2e-88
Identity = 168/318 (52.83%), Postives = 217/318 (68.24%), Query Frame = 0

Query: 116 HKVTRSFEY------------LQGDYYIAPVFMDKVVCHIVKNFIAHLLNIKVPLILGIW 175
           H V  S+EY            +   +YIAP FMDK+V HI KNF+  L NIKVPLILGIW
Sbjct: 106 HAVLSSYEYVSQGLRQYNLDNMMDGFYIAPAFMDKLVVHITKNFLT-LPNIKVPLILGIW 165

Query: 176 GGKGQGKSFQTELIFQTMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLS 235
           GGKGQGKSFQ EL+   MG+ P++MSAGELES  AGEP KLIR+RYR A+ +I+ +GK+ 
Sbjct: 166 GGKGQGKSFQCELVMAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIK-KGKMC 225

Query: 236 CLMINDIDAGIGRF-GNTQVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIIL 295
           CL IND+DAG GR  G TQ TVNNQ+   TLMN+ADNPT V +   + + +   R+PII 
Sbjct: 226 CLFINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEE-NARVPIIC 285

Query: 296 TGNDFSTIYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQAL 355
           TGNDFST+YAPLIRDGRMEKFYW P RED + +   ++  D I    ++ +V+ FP Q++
Sbjct: 286 TGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFRTDKIKDEDIVTLVDQFPGQSI 345

Query: 356 DFYGALRSRTYDAAVSKWVDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYS 415
           DF+GALR+R YD  V K+V+ + GVEK+G +L+  R  E  P F  PE T E L+E G  
Sbjct: 346 DFFGALRARVYDDEVRKFVESL-GVEKIGKRLVNSR--EGPPVFEQPEMTYEKLMEYGNM 405

Query: 416 LVKEQQLIMETKLSKEYM 421
           LV EQ+ +   +L++ Y+
Sbjct: 406 LVMEQENVKRVQLAETYL 417

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P934314.7e-9252.99Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Oryza sat... [more]
O490743.9e-9146.51Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Solanum p... [more]
Q405658.8e-9145.15Ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic OS=Nicotia... [more]
Q404603.3e-9044.87Ribulose bisphosphate carboxylase/oxygenase activase 1, chloroplastic OS=Nicotia... [more]
Q9ZT001.3e-8947.41Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Zea mays ... [more]
Match NameE-valueIdentityDescription
XP_022154094.14.92e-308100.00ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic isoform X1 [... [more]
XP_022960536.11.56e-28190.82ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic [Cucurbita m... [more]
XP_023515166.18.69e-28190.82ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic [Cucurbita p... [more]
XP_023004209.18.69e-28190.82ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic [Cucurbita m... [more]
XP_038900248.11.37e-27889.93ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic isoform X1 [... [more]
Match NameE-valueIdentityDescription
A0A6J1DL152.38e-308100.00ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic isoform X1 O... [more]
A0A6J1H7W07.56e-28290.82ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Cucurbita... [more]
A0A6J1KVM64.21e-28190.82ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Cucurbita... [more]
A0A5A7UYP68.70e-27788.26Ribulose bisphosphate carboxylase/oxygenase activase OS=Cucumis melo var. makuwa... [more]
A0A1S3CFE28.70e-27788.26ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Cucumis m... [more]
Match NameE-valueIdentityDescription
AT1G73110.12.6e-17874.02P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT2G39730.13.2e-8852.83rubisco activase [more]
AT2G39730.23.2e-8852.83rubisco activase [more]
AT2G39730.33.2e-8852.83rubisco activase [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003959ATPase, AAA-type, corePFAMPF00004AAAcoord: 162..304
e-value: 6.5E-12
score: 46.0
NoneNo IPR availableGENE3D1.10.8.1070coord: 308..423
e-value: 9.5E-40
score: 137.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 48..64
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 36..64
NoneNo IPR availablePANTHERPTHR32429:SF11OSJNBA0011F23.7 PROTEINcoord: 41..425
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 122..307
e-value: 1.2E-31
score: 111.7
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 152..330
IPR044960Ribulose bisphosphate carboxylase/oxygenase activase-likePANTHERPTHR32429FAMILY NOT NAMEDcoord: 41..425

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g0399.1MC09g0399.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity