MC09g0397 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC09g0397
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	mitogen-activated protein kinase kinase kinase 17-like
Location	MC09: 3591629 .. 3592967 (+)
RNA-Seq Expression	MC09g0397
Synteny	MC09g0397

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAGAAAGTCCACTGAAAATGACGAAATTGTCCCTACTCCGGTCAACCACGGCGTGCGGTGGCAGCGAGGGCGGCTGATCGGAAAAGGAAGCTTCGGGTCGGTTTTCATGGCGTCTTTACGGCGACCCATCTCTAAATTCAGCATTTTTCCTCCGGTGATGGCCGTCAAGTCCGCAGAAGTCTCCGATTCCGAAACCCTACAGAAGGAGAAGCAAAACTACGACAACTTGAAACAATGTGATTCGTTGATCAAATGCTTTGGAGAAGAGATCACCACCGACGAGAATGGCCAGATGATTTATAATCTGCTTCTCGAGTTCGCCACTGCCGGAACCCTAGCCGATCAAATCAAGAACACAGGTAAATTAAAGTAGTGATCATGGGCCAGAGCTCAACCAAAAAAAAAAAAAACATAAAATTTTTCATCTAACCCTAAAATCTTACATGTTAAGAAAAAATTTGACTGTAACCCTAAAATTTAGAACGGAATAAATTTAGGGTTTAATGAAATTTTTTATGGATTTACAGGTAATTTAAAGTAGTGATCATGGGGCGGAGCTAACAATAAAATTTTTCATCTAACCCTAAAAATCTTACATGTTTAGAATGTAATAAATTTTGGGTTTAATTTAGAATGGGAAAAAAACTACATTGGAATGTGAAATTTTGTGTGTCTTTAAGAAAGTTTTTTGATGGGTATTTTCAGGTGGAAAGGGTTTGGAGGAGAAGGAAGTTTGGAGCTACACAAGATCAATAATCAAAGGGCTAATTCACATTCACAAGAACCAATACGTGCACTGCGATTTGAAGCCGGAGAATATTTTGCTGGTGCCGAGGAATTCGGCCAACGGTCGGCGCTGCGTGGCGAAGATCGGCGATTTGGGGCTGGCGAAGAGGACCAGCAAGACAAAGAAAGACCGTTACTTGAGAGGAACGGTGCCGTACATGGCGCCGGAGACATTGCTCTACAGCGTGCAAGAATCGCCGAGTGATATTTGGGCGCTCGGCTGCGTCGTTCTGGAGATGCTCACCGGAAACCGCCCCTGGAGCGGCGGCGGCGGCGGCGACGACACGGAGGAGATTACGAGACGTTTTGGTGAGAAGTACGAGATTCCGATTATTCCGGAGGGAGCGGTGCTGTCGGCGGAGGCGAGGGCGTTTTTGAAGGTGTGTTTTGTGAGGAAGCCGGAGTATAGGTTCACGGCGGAGATGCTGATGTTGCTGCCGTTTTTGGCGGAGGACGAGGAGGAGGAAGAAGAATCTGTGGTGGGTGCTCCAAGATTTGTGGAGAAGGCTAGGAGGTCGAAGAGGCAAAGGATTCCAATTCAAGCTGTG

mRNA sequence

ATGAAGAGAAAGTCCACTGAAAATGACGAAATTGTCCCTACTCCGGTCAACCACGGCGTGCGGTGGCAGCGAGGGCGGCTGATCGGAAAAGGAAGCTTCGGGTCGGTTTTCATGGCGTCTTTACGGCGACCCATCTCTAAATTCAGCATTTTTCCTCCGGTGATGGCCGTCAAGTCCGCAGAAGTCTCCGATTCCGAAACCCTACAGAAGGAGAAGCAAAACTACGACAACTTGAAACAATGTGATTCGTTGATCAAATGCTTTGGAGAAGAGATCACCACCGACGAGAATGGCCAGATGATTTATAATCTGCTTCTCGAGTTCGCCACTGCCGGAACCCTAGCCGATCAAATCAAGAACACAGGTGGAAAGGGTTTGGAGGAGAAGGAAGTTTGGAGCTACACAAGATCAATAATCAAAGGGCTAATTCACATTCACAAGAACCAATACGTGCACTGCGATTTGAAGCCGGAGAATATTTTGCTGGTGCCGAGGAATTCGGCCAACGGTCGGCGCTGCGTGGCGAAGATCGGCGATTTGGGGCTGGCGAAGAGGACCAGCAAGACAAAGAAAGACCGTTACTTGAGAGGAACGGTGCCGTACATGGCGCCGGAGACATTGCTCTACAGCGTGCAAGAATCGCCGAGTGATATTTGGGCGCTCGGCTGCGTCGTTCTGGAGATGCTCACCGGAAACCGCCCCTGGAGCGGCGGCGGCGGCGGCGACGACACGGAGGAGATTACGAGACGTTTTGGTGAGAAGTACGAGATTCCGATTATTCCGGAGGGAGCGGTGCTGTCGGCGGAGGCGAGGGCGTTTTTGAAGGTGTGTTTTGTGAGGAAGCCGGAGTATAGGTTCACGGCGGAGATGCTGATGTTGCTGCCGTTTTTGGCGGAGGACGAGGAGGAGGAAGAAGAATCTGTGGTGGGTGCTCCAAGATTTGTGGAGAAGGCTAGGAGGTCGAAGAGGCAAAGGATTCCAATTCAAGCTGTG

Coding sequence (CDS)

ATGAAGAGAAAGTCCACTGAAAATGACGAAATTGTCCCTACTCCGGTCAACCACGGCGTGCGGTGGCAGCGAGGGCGGCTGATCGGAAAAGGAAGCTTCGGGTCGGTTTTCATGGCGTCTTTACGGCGACCCATCTCTAAATTCAGCATTTTTCCTCCGGTGATGGCCGTCAAGTCCGCAGAAGTCTCCGATTCCGAAACCCTACAGAAGGAGAAGCAAAACTACGACAACTTGAAACAATGTGATTCGTTGATCAAATGCTTTGGAGAAGAGATCACCACCGACGAGAATGGCCAGATGATTTATAATCTGCTTCTCGAGTTCGCCACTGCCGGAACCCTAGCCGATCAAATCAAGAACACAGGTGGAAAGGGTTTGGAGGAGAAGGAAGTTTGGAGCTACACAAGATCAATAATCAAAGGGCTAATTCACATTCACAAGAACCAATACGTGCACTGCGATTTGAAGCCGGAGAATATTTTGCTGGTGCCGAGGAATTCGGCCAACGGTCGGCGCTGCGTGGCGAAGATCGGCGATTTGGGGCTGGCGAAGAGGACCAGCAAGACAAAGAAAGACCGTTACTTGAGAGGAACGGTGCCGTACATGGCGCCGGAGACATTGCTCTACAGCGTGCAAGAATCGCCGAGTGATATTTGGGCGCTCGGCTGCGTCGTTCTGGAGATGCTCACCGGAAACCGCCCCTGGAGCGGCGGCGGCGGCGGCGACGACACGGAGGAGATTACGAGACGTTTTGGTGAGAAGTACGAGATTCCGATTATTCCGGAGGGAGCGGTGCTGTCGGCGGAGGCGAGGGCGTTTTTGAAGGTGTGTTTTGTGAGGAAGCCGGAGTATAGGTTCACGGCGGAGATGCTGATGTTGCTGCCGTTTTTGGCGGAGGACGAGGAGGAGGAAGAAGAATCTGTGGTGGGTGCTCCAAGATTTGTGGAGAAGGCTAGGAGGTCGAAGAGGCAAAGGATTCCAATTCAAGCTGTG

Protein sequence

MKRKSTENDEIVPTPVNHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSAEVSDSETLQKEKQNYDNLKQCDSLIKCFGEEITTDENGQMIYNLLLEFATAGTLADQIKNTGGKGLEEKEVWSYTRSIIKGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDLGLAKRTSKTKKDRYLRGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGGGDDTEEITRRFGEKYEIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAEDEEEEEESVVGAPRFVEKARRSKRQRIPIQAV

Homology

[Close]

BLAST of MC09g0397 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 3.2e-48
Identity = 117/289 (40.48%), Postives = 165/289 (57.09%), Query Frame = 0


Query: 20  VRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSAEVSDSETLQKEKQNYDNLK 79
           + W RG  IG G+F +V  A+  R       FP ++AVKS +   + +L  EK   D+L 
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSR---NSGDFPALIAVKSTDAYGAASLSNEKSVLDSLG 60

Query: 80  QCDSLIKCFGEEITTDENGQMIYNLLLEFATAGTLADQIKNTGGKGLEEKEVWSYTRSII 139
            C  +I+C+GE+ +T ENG+ ++NLLLE+A+ G+LA  +K  GG+GL E  V  +T S++
Sbjct: 61  DCPEIIRCYGED-STVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVL 120

Query: 140 KGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDLGLAKRT----SKTKKDRYL 199
           +GL HIH   + HCD+K  NILL    S        KI D GLA R     +  +K   +
Sbjct: 121 RGLRHIHAKGFAHCDIKLANILLFNDGS-------VKIADFGLAMRVDGDLTALRKSVEI 180

Query: 200 RGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGGGDDTEEITRRFGEKY 259
           RGT  YMAPE +  +   S +D+WALGC V+EM +G   WS    G     +  R G   
Sbjct: 181 RGTPLYMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWS-VKEGSHFMSLLIRIGVGD 240

Query: 260 EIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAEDEEEE 305
           E+P IPE  +LS E + FL  CFV+ P  R+TAEML+   F+  D E++
Sbjct: 241 ELPKIPE--MLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDD 275

[Close]

BLAST of MC09g0397 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 7.7e-42
Identity = 106/291 (36.43%), Postives = 155/291 (53.26%), Query Frame = 0


Query: 20  VRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSAEVSDSETLQKEKQNYDNLK 79
           + W RGR++G+GS  +V+ A+             ++AVKS+EV  SE LQ+E +   +L 
Sbjct: 1   MEWTRGRILGRGSTATVYAAAGHNS-------DEILAVKSSEVHRSEFLQREAKILSSLS 60

Query: 80  QCDSLIKCFGEEITTDENGQMIYNLLLEFATAGTLADQIKNTGGKGLEEKEVWSYTRSII 139
               +I   G E   + NG ++YNLL+E+A  GTL D     GG+ ++E  V  YTR I+
Sbjct: 61  S-PYVIGYRGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGR-VDETRVVKYTRDIL 120

Query: 140 KGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDLGLAKRTSKTKKDRYLRGTV 199
           KGL +IH    VHCD+K  N+++  +         AKI D G AKR     +   + GT 
Sbjct: 121 KGLEYIHSKGIVHCDVKGSNVVISEKGE-------AKIADFGCAKRVDPVFESPVM-GTP 180

Query: 200 PYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGGGDDTEEITRRFGEKYEIPI 259
            +MAPE      Q   SDIWA+GC ++EM+TG+ PW+     +D   +  R G   E P 
Sbjct: 181 AFMAPEVARGEKQGKESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPE 240

Query: 260 IPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAEDEEEEEESVVG 311
           +P   +L+ EA+ FL+ C  R+   R+TA  L+  PFL    + E   V G
Sbjct: 241 LP--CLLAEEAKDFLEKCLKREANERWTATQLLNHPFLTTKPDIEPVLVPG 272

[Close]

BLAST of MC09g0397 vs. ExPASy Swiss-Prot
Match: Q9ZVP5 (Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK18 PE=1 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 1.7e-33
Identity = 105/289 (36.33%), Postives = 150/289 (51.90%), Query Frame = 0


Query: 20  VRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSAEVSDSETLQKEKQNYDNLK 79
           + W RG+ +G+GS  +V  A+              +AVKSAE   SE LQ+E +   +L 
Sbjct: 1   MNWTRGKTLGRGSTATVSAATCHES-------GETLAVKSAEFHRSEFLQREAKILSSLN 60

Query: 80  QCDSLIKCFGEEITTD---ENGQ-MIYNLLLEFATAGTLADQIKNTGGKGLEEKEVWSYT 139
               +I   G EIT +    NG+   Y+LL+E+A  GTL D     GG  ++E  V  YT
Sbjct: 61  S-PYVIGYRGCEITREPFHNNGEATTYSLLMEYAPYGTLTDVATKNGG-FIDEARVVKYT 120

Query: 140 RSIIKGLIHIHKNQYV-HCDLKPENILLVPRNSANGRRCVAKIGDLGLAKRTSKTKKDRY 199
           R I+ GL +IH ++ + HCD+K  N+L+       G    AKI D G AK   + +    
Sbjct: 121 RQILLGLEYIHNSKGIAHCDIKGSNVLV-------GENGEAKIADFGCAKWV-EPEITEP 180

Query: 200 LRGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGGGDDTEEITRRFGEK 259
           +RGT  +MAPE      Q   SDIWA+GC V+EM+TG++PW G    D    +  R G  
Sbjct: 181 VRGTPAFMAPEAARGERQGKESDIWAVGCTVIEMVTGSQPWIGADFTDPV-SVLYRVGYL 240

Query: 260 YEIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAEDEEE 304
            E+P +P    L+ +A+ FL  C  ++   R+TA  L+  PFL   E E
Sbjct: 241 GELPELP--CSLTEQAKDFLGKCLKKEATERWTASQLLNHPFLVNKEPE 269

[Close]

BLAST of MC09g0397 vs. ExPASy Swiss-Prot
Match: P25390 (Serine/threonine-protein kinase SSK22 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SSK22 PE=1 SV=2)

HSP 1 Score: 130.2 bits (326), Expect = 4.4e-29
Identity = 99/313 (31.63%), Postives = 153/313 (48.88%), Query Frame = 0


Query: 17   NHGVRWQRGRLIGKGSFGSVFMA-SLRRPISKFSIFPPVMAVKSAEVSDSETLQ------ 76
            N  +RWQ+   IG G+FG V+ A +L            ++AVK  ++ D+ T++      
Sbjct: 1029 NVSMRWQKRSFIGGGTFGQVYSAINLEN--------GEILAVKEIKIHDTTTMKKIFPLI 1088

Query: 77   KEKQNYDNLKQCDSLIKCFGEEITTDENGQMIYNLLLEFATAGTLADQIKNTGGKGLEEK 136
            KE+     +    ++++ +G E+  D+      N+ +E+   G+LA  + +  G+  +E 
Sbjct: 1089 KEEMTVLEMLNHPNIVQYYGVEVHRDK-----VNIFMEYCEGGSLASLLDH--GRIEDEM 1148

Query: 137  EVWSYTRSIIKGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDLGLAK----- 196
                YT  +++GL ++H++  VH D+KPENILL      NG   + K  D G A+     
Sbjct: 1149 VTQVYTFELLEGLAYLHQSGVVHRDIKPENILL----DFNG---IIKYVDFGTARTVVGS 1208

Query: 197  RTS------------KTKKDRYLRGTVPYMAPETLLYSVQE---SPSDIWALGCVVLEML 256
            RT             +TK    + GT  YMAPET+  S  +      D+WALGCVVLEM 
Sbjct: 1209 RTRTVRNAAVQDFGVETKSLNEMMGTPMYMAPETISGSAVKGKLGADDVWALGCVVLEMA 1268

Query: 257  TGNRPWSGGGGGDDTEEITRRFGEKYEIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAE 303
            TG RPWS      D E           IP +P    ++A  RAFL+ C V+ P  R TA 
Sbjct: 1269 TGRRPWS----NLDNEWAIMYHVAAGRIPQLPNRDEMTAAGRAFLERCLVQDPTMRATAV 1315

[Close]

BLAST of MC09g0397 vs. ExPASy Swiss-Prot
Match: Q54R82 (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=44689 GN=mkkA PE=1 SV=2)

HSP 1 Score: 125.6 bits (314), Expect = 1.1e-27
Identity = 85/302 (28.15%), Postives = 149/302 (49.34%), Query Frame = 0


Query: 7   ENDEIVPTPVNHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSAEVSDSE 66
           E++E++    NH ++WQ+G+++G+G +GSV++  L +   +      + AVK  E+ D  
Sbjct: 158 EHEELIS---NHNIKWQKGQILGRGGYGSVYL-GLNKDTGE------LFAVKQLEIVDIN 217

Query: 67  ----------TLQKEKQNYDNLKQCDSLIKCFGEEITTDENGQMIYNLLLEFATAGTLAD 126
                     +  KE +   +L+  D++++  G  +      Q   ++ LE+   G+++ 
Sbjct: 218 SDPKLKNMILSFSKEIEVMRSLRH-DNIVRYLGTSL-----DQSFLSVFLEYIPGGSISS 277

Query: 127 QIKNTGGKGLEEKEVWSYTRSIIKGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAK 186
            +   G     E  +  YT+ I++GL  +H N  +H D+K  NIL+  +        + K
Sbjct: 278 LLGKFG--AFSENVIKVYTKQILQGLSFLHANSIIHRDIKGANILIDTKG-------IVK 337

Query: 187 IGDLGLAKRTSK-TKKDRYLRGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPW 246
           + D G +K  S    + + ++GT  +MAPE +  +     SDIW+LGCV++EM T   PW
Sbjct: 338 LSDFGCSKSFSGIVSQFKSMQGTPYWMAPEVIKQTGHGRSSDIWSLGCVIVEMATAQPPW 397

Query: 247 SGGGGGDDTEEITRRFGEKYEIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLP 298
           S      +   +         IP IP  + +S EA  FL +CF R P+ R  A  L+  P
Sbjct: 398 S---NITELAAVMYHIASSNSIPNIP--SHMSQEAFDFLNLCFKRDPKERPDANQLLKHP 429

[Close]

BLAST of MC09g0397 vs. NCBI nr
Match: XP_022154776.1 (mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia])

HSP 1 Score: 660 bits (1703), Expect = 4.00e-239
Identity = 331/331 (100.00%), Postives = 331/331 (100.00%), Query Frame = 0


Query: 1   MKRKSTENDEIVPTPVNHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSA 60
           MKRKSTENDEIVPTPVNHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSA
Sbjct: 1   MKRKSTENDEIVPTPVNHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSA 60

Query: 61  EVSDSETLQKEKQNYDNLKQCDSLIKCFGEEITTDENGQMIYNLLLEFATAGTLADQIKN 120
           EVSDSETLQKEKQNYDNLKQCDSLIKCFGEEITTDENGQMIYNLLLEFATAGTLADQIKN
Sbjct: 61  EVSDSETLQKEKQNYDNLKQCDSLIKCFGEEITTDENGQMIYNLLLEFATAGTLADQIKN 120

Query: 121 TGGKGLEEKEVWSYTRSIIKGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDL 180
           TGGKGLEEKEVWSYTRSIIKGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDL
Sbjct: 121 TGGKGLEEKEVWSYTRSIIKGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDL 180

Query: 181 GLAKRTSKTKKDRYLRGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGG 240
           GLAKRTSKTKKDRYLRGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGG
Sbjct: 181 GLAKRTSKTKKDRYLRGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGG 240

Query: 241 GDDTEEITRRFGEKYEIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAED 300
           GDDTEEITRRFGEKYEIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAED
Sbjct: 241 GDDTEEITRRFGEKYEIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAED 300

Query: 301 EEEEEESVVGAPRFVEKARRSKRQRIPIQAV 331
           EEEEEESVVGAPRFVEKARRSKRQRIPIQAV
Sbjct: 301 EEEEEESVVGAPRFVEKARRSKRQRIPIQAV 331

[Close]

BLAST of MC09g0397 vs. NCBI nr
Match: XP_008461430.1 (PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo])

HSP 1 Score: 397 bits (1020), Expect = 4.39e-135
Identity = 219/335 (65.37%), Postives = 257/335 (76.72%), Query Frame = 0


Query: 1   MKRKSTENDEIVPTPVNHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSA 60
           M  K     E   T  N+G++W+RGRLIGKGSFGSVF+A L+   +K+SIFPPVMAVKSA
Sbjct: 6   MLEKEAAQVEEGSTEFNNGIQWKRGRLIGKGSFGSVFLACLKPHFTKYSIFPPVMAVKSA 65

Query: 61  EVSDSETLQKEKQNYDNLKQCDSLIKCFGEEITTDENGQMIYNLLLEFATAGTLADQIKN 120
           E+S SETLQKEKQNYDNLK C+SLI+CFGEEITTD NG+MIYNLLLE AT GTLA  IKN
Sbjct: 66  EISVSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHNGRMIYNLLLEVATGGTLAHHIKN 125

Query: 121 TGGKGLEEKEVWSYTRSIIKGLIHIHKNQYVHCDLKPENILLVPRNSAN-GRRCVAKIGD 180
           TGGKGLEE  V +YT+SIIKGLIHIH++QYVHCDLKP NILL+P+N+    R+ +AKI D
Sbjct: 126 TGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLLPKNNTTKDRQFIAKIAD 185

Query: 181 LGLAKRTSKTKKDRYLRGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGG 240
           LGLA+RTSKTK    L GT  YMAPETL+  VQES SDIWALGCVVLEMLTGNR W+   
Sbjct: 186 LGLARRTSKTKASYCLGGTFSYMAPETLIDGVQESTSDIWALGCVVLEMLTGNRAWAATD 245

Query: 241 GGDDTEEITRRFGEKYEIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAE 300
                +E+T  F     +P IPEG  LSAEA  FLK C VRKPE+RFTAEML+ +PF+A 
Sbjct: 246 KVGILQEMTENF---LGMPKIPEG--LSAEATGFLKNCLVRKPEFRFTAEMLINVPFVAA 305

Query: 301 DEEEEEE-SVVGAPRFVEK-ARRSKRQRI-PIQAV 331
            E++E++ + V AP FV K  R+ KRQRI PI+AV
Sbjct: 306 AEDQEQDFNTVKAPTFVTKWPRQFKRQRIIPIKAV 335

[Close]

BLAST of MC09g0397 vs. NCBI nr
Match: XP_004136102.2 (mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus])

HSP 1 Score: 396 bits (1018), Expect = 8.83e-135
Identity = 221/340 (65.00%), Postives = 258/340 (75.88%), Query Frame = 0


Query: 1   MKRKSTENDEIVP-----TPVNHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVM 60
           MKRKS    E        +  N+G++W++GRLIGKGSFGSVF+ASL+   +K+SIFPPVM
Sbjct: 1   MKRKSMLEKEAAQVEDRSSEFNNGIQWKQGRLIGKGSFGSVFLASLKPHFTKYSIFPPVM 60

Query: 61  AVKSAEVSDSETLQKEKQNYDNLKQCDSLIKCFGEEITTDENGQMIYNLLLEFATAGTLA 120
           AVKSAE+S SETLQKEKQNYDNLK C+SLI+CFGEEITTD NG MIYNLLLE AT GTLA
Sbjct: 61  AVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHNGHMIYNLLLEVATGGTLA 120

Query: 121 DQIKNTGGKGLEEKEVWSYTRSIIKGLIHIHKNQYVHCDLKPENILLVPRNSAN-GRRCV 180
             IKNTGGKGLEE  V +YT+SIIKGLIH+H++QYVHCDLKP NILL+P+N+    R+ +
Sbjct: 121 HHIKNTGGKGLEENVVRNYTKSIIKGLIHMHRSQYVHCDLKPANILLLPKNNTTKDRQFI 180

Query: 181 AKIGDLGLAKRTSKTKKDRYLRGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRP 240
           AKI DLGLA+RTSKTK    L GT  YMAPETL+  VQES SDIWALGCVVLEMLTGNR 
Sbjct: 181 AKIADLGLARRTSKTKASYCLGGTFSYMAPETLIDGVQESASDIWALGCVVLEMLTGNRA 240

Query: 241 WSGGGGGDDTEEITRRFGEKYEIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLL 300
           W+        +E+T  F     +P IPEG  LS EA  FLK C VRKPE+RFTAEMLM +
Sbjct: 241 WAATDKVGIVKEMTENF---IGMPKIPEG--LSPEATGFLKNCLVRKPEFRFTAEMLMNV 300

Query: 301 PFLAEDEEEEEESV-VGAPRFVEK-ARRSKRQR-IPIQAV 331
           PF+A  E+ E++S  V AP FV K  R+ KRQR IPI+AV
Sbjct: 301 PFVAAGEDHEQDSTTVKAPTFVTKWPRQFKRQRTIPIKAV 335

[Close]

BLAST of MC09g0397 vs. NCBI nr
Match: KAE8646420.1 (hypothetical protein Csa_015819 [Cucumis sativus])

HSP 1 Score: 395 bits (1014), Expect = 3.01e-134
Identity = 218/335 (65.07%), Postives = 255/335 (76.12%), Query Frame = 0


Query: 1   MKRKSTENDEIVPTPVNHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSA 60
           M  K     E   +  N+G++W++GRLIGKGSFGSVF+ASL+   +K+SIFPPVMAVKSA
Sbjct: 1   MLEKEAAQVEDRSSEFNNGIQWKQGRLIGKGSFGSVFLASLKPHFTKYSIFPPVMAVKSA 60

Query: 61  EVSDSETLQKEKQNYDNLKQCDSLIKCFGEEITTDENGQMIYNLLLEFATAGTLADQIKN 120
           E+S SETLQKEKQNYDNLK C+SLI+CFGEEITTD NG MIYNLLLE AT GTLA  IKN
Sbjct: 61  EISVSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHNGHMIYNLLLEVATGGTLAHHIKN 120

Query: 121 TGGKGLEEKEVWSYTRSIIKGLIHIHKNQYVHCDLKPENILLVPRNSAN-GRRCVAKIGD 180
           TGGKGLEE  V +YT+SIIKGLIH+H++QYVHCDLKP NILL+P+N+    R+ +AKI D
Sbjct: 121 TGGKGLEENVVRNYTKSIIKGLIHMHRSQYVHCDLKPANILLLPKNNTTKDRQFIAKIAD 180

Query: 181 LGLAKRTSKTKKDRYLRGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGG 240
           LGLA+RTSKTK    L GT  YMAPETL+  VQES SDIWALGCVVLEMLTGNR W+   
Sbjct: 181 LGLARRTSKTKASYCLGGTFSYMAPETLIDGVQESASDIWALGCVVLEMLTGNRAWAATD 240

Query: 241 GGDDTEEITRRFGEKYEIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAE 300
                +E+T  F     +P IPEG  LS EA  FLK C VRKPE+RFTAEMLM +PF+A 
Sbjct: 241 KVGIVKEMTENF---IGMPKIPEG--LSPEATGFLKNCLVRKPEFRFTAEMLMNVPFVAA 300

Query: 301 DEEEEEESV-VGAPRFVEK-ARRSKRQR-IPIQAV 331
            E+ E++S  V AP FV K  R+ KRQR IPI+AV
Sbjct: 301 GEDHEQDSTTVKAPTFVTKWPRQFKRQRTIPIKAV 330

[Close]

BLAST of MC09g0397 vs. NCBI nr
Match: KAG7014197.1 (Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 395 bits (1015), Expect = 5.03e-134
Identity = 216/327 (66.06%), Postives = 248/327 (75.84%), Query Frame = 0


Query: 17  NHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSAEVSDSETLQKEKQNYD 76
           N GV+W+RGRLIGKGSFGSVFMASL+R  ++FSIFPPVMAVKSAEVS S++LQKEKQ YD
Sbjct: 26  NDGVQWERGRLIGKGSFGSVFMASLKRSTAEFSIFPPVMAVKSAEVSVSKSLQKEKQIYD 85

Query: 77  NLKQCDSLIKCFGEEITTDENGQMIYNLLLEFATAGTLADQIKNTGGKGLEEKEVWSYTR 136
           NLK C+SLI+CFGEEITTD NG MIYNLLLE A  G+LA +I NTGGKGL E EV  YT+
Sbjct: 86  NLKGCNSLIQCFGEEITTDRNGPMIYNLLLEIALGGSLAHRINNTGGKGLAENEVGRYTK 145

Query: 137 SIIKGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDLGLAKRTSKTKKDRYLR 196
           +IIKGLIHIH+  YVHCDLKP NILL+P N+A G R VAKI DLGLA+RTS+T+      
Sbjct: 146 TIIKGLIHIHRRGYVHCDLKPANILLLPTNTAKGGRLVAKICDLGLARRTSETRHGNCFG 205

Query: 197 GTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGGGDDTEEITRRFGEKYE 256
           GT+ YMAPETL++ VQESPSDIWALGC+VLEMLTGNRPW+      D   I +   E   
Sbjct: 206 GTLSYMAPETLIHRVQESPSDIWALGCLVLEMLTGNRPWAA----TDEAGIVKSITENDG 265

Query: 257 IPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLA---------EDEEEEEES 316
           +P IPEG  +S EA  FLK CFVR PEYRFTAEMLM +PF+A         E+EEEEEE 
Sbjct: 266 MPEIPEG--VSKEASGFLKNCFVRNPEYRFTAEMLMTVPFVAAEEEEEEEEEEEEEEEED 325

Query: 317 VVGAPRFVEKARRS--KRQRI-PIQAV 331
            + A  FV K  RS  KRQRI P +AV
Sbjct: 326 FMRALTFVRKRSRSCRKRQRIVPTEAV 346

[Close]

BLAST of MC09g0397 vs. ExPASy TrEMBL
Match: A0A6J1DPQ9 (mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX=3673 GN=LOC111021943 PE=3 SV=1)

HSP 1 Score: 660 bits (1703), Expect = 1.94e-239
Identity = 331/331 (100.00%), Postives = 331/331 (100.00%), Query Frame = 0


Query: 1   MKRKSTENDEIVPTPVNHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSA 60
           MKRKSTENDEIVPTPVNHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSA
Sbjct: 1   MKRKSTENDEIVPTPVNHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSA 60

Query: 61  EVSDSETLQKEKQNYDNLKQCDSLIKCFGEEITTDENGQMIYNLLLEFATAGTLADQIKN 120
           EVSDSETLQKEKQNYDNLKQCDSLIKCFGEEITTDENGQMIYNLLLEFATAGTLADQIKN
Sbjct: 61  EVSDSETLQKEKQNYDNLKQCDSLIKCFGEEITTDENGQMIYNLLLEFATAGTLADQIKN 120

Query: 121 TGGKGLEEKEVWSYTRSIIKGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDL 180
           TGGKGLEEKEVWSYTRSIIKGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDL
Sbjct: 121 TGGKGLEEKEVWSYTRSIIKGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDL 180

Query: 181 GLAKRTSKTKKDRYLRGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGG 240
           GLAKRTSKTKKDRYLRGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGG
Sbjct: 181 GLAKRTSKTKKDRYLRGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGG 240

Query: 241 GDDTEEITRRFGEKYEIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAED 300
           GDDTEEITRRFGEKYEIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAED
Sbjct: 241 GDDTEEITRRFGEKYEIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAED 300

Query: 301 EEEEEESVVGAPRFVEKARRSKRQRIPIQAV 331
           EEEEEESVVGAPRFVEKARRSKRQRIPIQAV
Sbjct: 301 EEEEEESVVGAPRFVEKARRSKRQRIPIQAV 331

[Close]

BLAST of MC09g0397 vs. ExPASy TrEMBL
Match: A0A0A0KBM3 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G430800 PE=3 SV=1)

HSP 1 Score: 397 bits (1021), Expect = 1.50e-135
Identity = 222/340 (65.29%), Postives = 258/340 (75.88%), Query Frame = 0


Query: 1   MKRKSTENDEIVP-----TPVNHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVM 60
           MKRKS    E        +  N+G++W++GRLIGKGSFGSVF+ASL+   +K+SIFPPVM
Sbjct: 1   MKRKSMLEKEAAQVEDRSSEFNNGIQWKQGRLIGKGSFGSVFLASLKPHFTKYSIFPPVM 60

Query: 61  AVKSAEVSDSETLQKEKQNYDNLKQCDSLIKCFGEEITTDENGQMIYNLLLEFATAGTLA 120
           AVKSAE+S SETLQKEKQNYDNLK C+SLI+CFGEEITTD NG MIYNLLLE AT GTLA
Sbjct: 61  AVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHNGHMIYNLLLEVATGGTLA 120

Query: 121 DQIKNTGGKGLEEKEVWSYTRSIIKGLIHIHKNQYVHCDLKPENILLVPRNSAN-GRRCV 180
             IKNTGGKGLEE  V +YT+SIIKGLIHIH++QYVHCDLKP NILL+P+N+    R+ +
Sbjct: 121 HHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLLPKNNTTKDRQFI 180

Query: 181 AKIGDLGLAKRTSKTKKDRYLRGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRP 240
           AKI DLGLA+RTSKTK    L GT  YMAPETL+  VQES SDIWALGCVVLEMLTGNR 
Sbjct: 181 AKIADLGLARRTSKTKASYCLGGTFSYMAPETLIDGVQESASDIWALGCVVLEMLTGNRA 240

Query: 241 WSGGGGGDDTEEITRRFGEKYEIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLL 300
           W+        +E+T  F     +P IPEG  LS EA  FLK C VRKPE+RFTAEMLM +
Sbjct: 241 WAATDKVGIVKEMTENF---IGMPKIPEG--LSPEATGFLKNCLVRKPEFRFTAEMLMNV 300

Query: 301 PFLAEDEEEEEESV-VGAPRFVEK-ARRSKRQR-IPIQAV 331
           PF+A  E+ E++S  V AP FV K  R+ KRQR IPI+AV
Sbjct: 301 PFVAAGEDHEQDSTTVKAPTFVTKWPRQFKRQRTIPIKAV 335

[Close]

BLAST of MC09g0397 vs. ExPASy TrEMBL
Match: A0A1S3CFZ6 (mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo OX=3656 GN=LOC103500025 PE=3 SV=1)

HSP 1 Score: 397 bits (1020), Expect = 2.12e-135
Identity = 219/335 (65.37%), Postives = 257/335 (76.72%), Query Frame = 0


Query: 1   MKRKSTENDEIVPTPVNHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSA 60
           M  K     E   T  N+G++W+RGRLIGKGSFGSVF+A L+   +K+SIFPPVMAVKSA
Sbjct: 6   MLEKEAAQVEEGSTEFNNGIQWKRGRLIGKGSFGSVFLACLKPHFTKYSIFPPVMAVKSA 65

Query: 61  EVSDSETLQKEKQNYDNLKQCDSLIKCFGEEITTDENGQMIYNLLLEFATAGTLADQIKN 120
           E+S SETLQKEKQNYDNLK C+SLI+CFGEEITTD NG+MIYNLLLE AT GTLA  IKN
Sbjct: 66  EISVSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHNGRMIYNLLLEVATGGTLAHHIKN 125

Query: 121 TGGKGLEEKEVWSYTRSIIKGLIHIHKNQYVHCDLKPENILLVPRNSAN-GRRCVAKIGD 180
           TGGKGLEE  V +YT+SIIKGLIHIH++QYVHCDLKP NILL+P+N+    R+ +AKI D
Sbjct: 126 TGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLLPKNNTTKDRQFIAKIAD 185

Query: 181 LGLAKRTSKTKKDRYLRGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGG 240
           LGLA+RTSKTK    L GT  YMAPETL+  VQES SDIWALGCVVLEMLTGNR W+   
Sbjct: 186 LGLARRTSKTKASYCLGGTFSYMAPETLIDGVQESTSDIWALGCVVLEMLTGNRAWAATD 245

Query: 241 GGDDTEEITRRFGEKYEIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAE 300
                +E+T  F     +P IPEG  LSAEA  FLK C VRKPE+RFTAEML+ +PF+A 
Sbjct: 246 KVGILQEMTENF---LGMPKIPEG--LSAEATGFLKNCLVRKPEFRFTAEMLINVPFVAA 305

Query: 301 DEEEEEE-SVVGAPRFVEK-ARRSKRQRI-PIQAV 331
            E++E++ + V AP FV K  R+ KRQRI PI+AV
Sbjct: 306 AEDQEQDFNTVKAPTFVTKWPRQFKRQRIIPIKAV 335

[Close]

BLAST of MC09g0397 vs. ExPASy TrEMBL
Match: A0A5D3CH22 (Mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001440 PE=3 SV=1)

HSP 1 Score: 392 bits (1008), Expect = 1.41e-133
Identity = 217/335 (64.78%), Postives = 255/335 (76.12%), Query Frame = 0


Query: 1   MKRKSTENDEIVPTPVNHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSA 60
           M  K     E   +  N+G++W+RGRLIGKGSFGSVF+A L+    K+SIFPPVMAVKSA
Sbjct: 6   MLEKEAAQVEEGSSEFNNGIQWKRGRLIGKGSFGSVFLACLKPHFIKYSIFPPVMAVKSA 65

Query: 61  EVSDSETLQKEKQNYDNLKQCDSLIKCFGEEITTDENGQMIYNLLLEFATAGTLADQIKN 120
           E+S SETLQKEKQNYDNLK C+SLI+CFGEEITTD NG+M YNLLLE AT GTLA  IKN
Sbjct: 66  EISVSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHNGRMTYNLLLEVATGGTLAHHIKN 125

Query: 121 TGGKGLEEKEVWSYTRSIIKGLIHIHKNQYVHCDLKPENILLVPRNSAN-GRRCVAKIGD 180
           TGGKGLEE  V +YT+SIIKGLIHIH++QYVHCDLKP NILL+P+N+    R+ +AKI D
Sbjct: 126 TGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLLPKNNTTKDRQFIAKIAD 185

Query: 181 LGLAKRTSKTKKDRYLRGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGG 240
           LGLA+RTSKTK    L GT  YMAPETL+  VQES SDIWALGCVVLEMLTGNR W+   
Sbjct: 186 LGLARRTSKTKGSYCLGGTFSYMAPETLIDGVQESTSDIWALGCVVLEMLTGNRAWAATD 245

Query: 241 GGDDTEEITRRFGEKYEIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAE 300
                +E+T  F     +P IPEG  LSAEA  FLK C VRKPE+RFTAEML+ +PF+A 
Sbjct: 246 KVGIVQEMTENF---LGMPKIPEG--LSAEATGFLKNCLVRKPEFRFTAEMLINVPFVAA 305

Query: 301 DEEEEEE-SVVGAPRFVEK-ARRSKRQRI-PIQAV 331
            E++E++ + V AP FV K  R+ KRQRI PI+AV
Sbjct: 306 AEDQEQDFNTVKAPTFVTKWPRQFKRQRIIPIKAV 335

[Close]

BLAST of MC09g0397 vs. ExPASy TrEMBL
Match: A0A6J1GPL4 (mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=3662 GN=LOC111456326 PE=3 SV=1)

HSP 1 Score: 389 bits (998), Expect = 6.57e-132
Identity = 214/323 (66.25%), Postives = 247/323 (76.47%), Query Frame = 0


Query: 17  NHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSAEVSDSETLQKEKQNYD 76
           N GV+W+RGRLIGKGSFGSVFMASL+R  S+FSIFPPVMAVKSAEVS S++LQKEKQ YD
Sbjct: 22  NDGVQWERGRLIGKGSFGSVFMASLKR--SEFSIFPPVMAVKSAEVSVSKSLQKEKQIYD 81

Query: 77  NLKQCDSLIKCFGEEITTDENGQMIYNLLLEFATAGTLADQIKNTGGKGLEEKEVWSYTR 136
           NLK C+SLI+CFGEEITTD NG+MIYNL LE A  G+LA +I N GGKGL E EV  YT+
Sbjct: 82  NLKGCNSLIQCFGEEITTDRNGRMIYNLRLEIALGGSLAHRINNIGGKGLAENEVGRYTK 141

Query: 137 SIIKGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDLGLAKRTSKTKKDRYLR 196
           +IIKGLIHIH+  YVHCDLKP NILL+P N+A G R VAKI DLGLA+RTS+T+      
Sbjct: 142 TIIKGLIHIHRRGYVHCDLKPANILLLPTNTAKGGRLVAKICDLGLARRTSETRHGNCFG 201

Query: 197 GTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGGGDDTEEITRRFGEKYE 256
           GT+ YMAPETL++ VQESPSDIWALGC+VLEMLTGNRPW+      D   I +   E   
Sbjct: 202 GTLSYMAPETLIHRVQESPSDIWALGCLVLEMLTGNRPWAA----TDEAGIVKSITENDG 261

Query: 257 IPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLA-----EDEEEEEESVVGA 316
           +P IPEG  +S +A  FLK CFVR PEYRFTAEMLM +PF+A     E+EEEEEE  + A
Sbjct: 262 MPEIPEG--VSKDASGFLKNCFVRNPEYRFTAEMLMTVPFVAAAEEEEEEEEEEEDFMRA 321

Query: 317 PRFVEKARRS--KRQRI-PIQAV 331
             FV K  RS  KRQRI P +AV
Sbjct: 322 LTFVRKRSRSCRKRQRIVPTEAV 336

[Close]

BLAST of MC09g0397 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 211.8 bits (538), Expect = 8.1e-55
Identity = 132/297 (44.44%), Postives = 179/297 (60.27%), Query Frame = 0


Query: 20  VRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSAEVSDSETLQKEKQNYDNLK 79
           + W R   IG GSF +V +A+     SK   FP +MAVKS+ V  S  L+ E+   D+L 
Sbjct: 1   MEWIRRETIGHGSFSTVSLATTSGSSSK--AFPSLMAVKSSGVVCSAALRNERDVLDDLG 60

Query: 80  QCDSLIKCFGEEITTDENGQMIYNLLLEFATAGTLADQIKNTGGKGLEEKEVWSYTRSII 139
            C  +++CFGE  T  ENG+ IYNL LE+A+ G+LAD+IK++ G+ L E EV  +TRSI+
Sbjct: 61  DCSEIVRCFGEGRTV-ENGEEIYNLFLEYASGGSLADRIKSS-GEALPEFEVRRFTRSIV 120

Query: 140 KGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDLGLAKRTSKTKKDRYLRGTV 199
           KGL HIH N + HCD+K EN+L+             KI D GLAKR S  +    +RGT 
Sbjct: 121 KGLCHIHGNGFTHCDIKLENVLVFGDGD-------VKISDFGLAKRRS-GEVCVEIRGTP 180

Query: 200 PYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWS-GGGGGDDTEEITRRFGEKYEIP 259
            YMAPE++ +   ESP+DIWALGC V+EM +G   W    G  ++   +  R G   E+P
Sbjct: 181 LYMAPESVNHGEFESPADIWALGCSVVEMSSGKTAWCLEDGVMNNVMSLLVRIGSGDEVP 240

Query: 260 IIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAEDEE--EEEESVVGAPR 314
            IP    LS E + F+  CFV+    R+TAEML+  PFLA D+E  EE+E+   +PR
Sbjct: 241 RIP--VELSEEGKDFVSKCFVKNAAERWTAEMLLDHPFLAVDDESGEEDEACSVSPR 283

[Close]

BLAST of MC09g0397 vs. TAIR 10
Match: AT5G67080.1 (mitogen-activated protein kinase kinase kinase 19 )

HSP 1 Score: 201.4 bits (511), Expect = 1.1e-51
Identity = 124/299 (41.47%), Postives = 174/299 (58.19%), Query Frame = 0


Query: 20  VRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSAEVSDSETLQKEKQNYDNL- 79
           + W RG  IG G+F +V +A+  R  +    FPP+MAVKSA+   + +L  EK   DNL 
Sbjct: 1   MEWIRGETIGYGTFSTVSLAT--RSNNDSGEFPPLMAVKSADSYGAASLANEKSVLDNLG 60

Query: 80  KQCDSLIKCFGEEITTDENGQMIYNLLLEFATAGTLADQIKNTGGKGLEEKEVWSYTRSI 139
             C+ +++CFGE+ T  ENG+ ++NL LE+A+ G+L   +K   G+G+ E  V  +T S+
Sbjct: 61  DDCNEIVRCFGEDRTV-ENGEEMHNLFLEYASRGSLESYLKKLAGEGVPESTVRRHTGSV 120

Query: 140 IKGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDLGLAKRTSKTKKDRY---L 199
           ++GL HIH N + HCDLK  NILL    +        KI D GLAKR        Y   +
Sbjct: 121 LRGLRHIHANGFAHCDLKLGNILLFGDGA-------VKIADFGLAKRIGDLTALNYGVQI 180

Query: 200 RGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGGGDDTEEITRRFGEKY 259
           RGT  YMAPE++  +   S  D+WALGCVV+EM +G   WS    G +   +  R G   
Sbjct: 181 RGTPLYMAPESVNDNEYGSEGDVWALGCVVVEMFSGKTAWS-LKEGSNFMSLLLRIGVGD 240

Query: 260 EIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAEDEE-----EEEESVV 310
           E+P+IPE   LS + R FL  CFV+ P+ R+TAEML+  PF+  D +     +EE+ VV
Sbjct: 241 EVPMIPE--ELSEQGRDFLSKCFVKDPKKRWTAEMLLNHPFVTVDVDHDVLVKEEDFVV 286

[Close]

BLAST of MC09g0397 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 193.7 bits (491), Expect = 2.3e-49
Identity = 117/289 (40.48%), Postives = 165/289 (57.09%), Query Frame = 0


Query: 20  VRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSAEVSDSETLQKEKQNYDNLK 79
           + W RG  IG G+F +V  A+  R       FP ++AVKS +   + +L  EK   D+L 
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSR---NSGDFPALIAVKSTDAYGAASLSNEKSVLDSLG 60

Query: 80  QCDSLIKCFGEEITTDENGQMIYNLLLEFATAGTLADQIKNTGGKGLEEKEVWSYTRSII 139
            C  +I+C+GE+ +T ENG+ ++NLLLE+A+ G+LA  +K  GG+GL E  V  +T S++
Sbjct: 61  DCPEIIRCYGED-STVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVL 120

Query: 140 KGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDLGLAKRT----SKTKKDRYL 199
           +GL HIH   + HCD+K  NILL    S        KI D GLA R     +  +K   +
Sbjct: 121 RGLRHIHAKGFAHCDIKLANILLFNDGS-------VKIADFGLAMRVDGDLTALRKSVEI 180

Query: 200 RGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGGGDDTEEITRRFGEKY 259
           RGT  YMAPE +  +   S +D+WALGC V+EM +G   WS    G     +  R G   
Sbjct: 181 RGTPLYMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWS-VKEGSHFMSLLIRIGVGD 240

Query: 260 EIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAEDEEEE 305
           E+P IPE  +LS E + FL  CFV+ P  R+TAEML+   F+  D E++
Sbjct: 241 ELPKIPE--MLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDD 275

[Close]

BLAST of MC09g0397 vs. TAIR 10
Match: AT4G26890.1 (mitogen-activated protein kinase kinase kinase 16 )

HSP 1 Score: 182.2 bits (461), Expect = 6.9e-46
Identity = 112/285 (39.30%), Postives = 160/285 (56.14%), Query Frame = 0


Query: 20  VRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSAEVSDSETLQKEKQNYDNLK 79
           + W RG +IG+GS  +V +A         S    + AVKSA++S S  LQKE+     L 
Sbjct: 3   INWTRGPIIGRGSTATVSIA--------ISSSGELFAVKSADLSSSSLLQKEQSILSTLS 62

Query: 80  QCDSLIKCFGEEITTDENGQMIYNLLLEFATAGTLADQIKNTGGKGLEEKEVWSYTRSII 139
               ++K  G  +T + NG ++YN+L+E+ + G L D IKN+GGK L E E+ SYTR I+
Sbjct: 63  S-PHMVKYIGTGLTRESNG-LVYNILMEYVSGGNLHDLIKNSGGK-LPEPEIRSYTRQIL 122

Query: 140 KGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDLGLAKRTSKTKKDRYLRGTV 199
            GL+++H+   VHCDLK  N+L+      NG   V KI D+G AK   K++      GT 
Sbjct: 123 NGLVYLHERGIVHCDLKSHNVLV----EENG---VLKIADMGCAKSVDKSE----FSGTP 182

Query: 200 PYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGGGDDTEEITRRFGEKYEIPI 259
            +MAPE      Q  P+D+WALGC ++EM+TG+ PW      +D      + G   E P 
Sbjct: 183 AFMAPEVARGEEQRFPADVWALGCTMIEMMTGSSPWP---ELNDVVAAMYKIGFSGESPA 242

Query: 260 IPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAEDEEEE 305
           IP  A +S +A+ FLK C     + R+T E L+  PFL +DEE +
Sbjct: 243 IP--AWISDKAKDFLKNCLKEDQKQRWTVEELLKHPFLDDDEESQ 260

[Close]

BLAST of MC09g0397 vs. TAIR 10
Match: AT2G32510.1 (mitogen-activated protein kinase kinase kinase 17 )

HSP 1 Score: 172.6 bits (436), Expect = 5.4e-43
Identity = 106/291 (36.43%), Postives = 155/291 (53.26%), Query Frame = 0


Query: 20  VRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSAEVSDSETLQKEKQNYDNLK 79
           + W RGR++G+GS  +V+ A+             ++AVKS+EV  SE LQ+E +   +L 
Sbjct: 1   MEWTRGRILGRGSTATVYAAAGHNS-------DEILAVKSSEVHRSEFLQREAKILSSLS 60

Query: 80  QCDSLIKCFGEEITTDENGQMIYNLLLEFATAGTLADQIKNTGGKGLEEKEVWSYTRSII 139
               +I   G E   + NG ++YNLL+E+A  GTL D     GG+ ++E  V  YTR I+
Sbjct: 61  S-PYVIGYRGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGR-VDETRVVKYTRDIL 120

Query: 140 KGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDLGLAKRTSKTKKDRYLRGTV 199
           KGL +IH    VHCD+K  N+++  +         AKI D G AKR     +   + GT 
Sbjct: 121 KGLEYIHSKGIVHCDVKGSNVVISEKGE-------AKIADFGCAKRVDPVFESPVM-GTP 180

Query: 200 PYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGGGDDTEEITRRFGEKYEIPI 259
            +MAPE      Q   SDIWA+GC ++EM+TG+ PW+     +D   +  R G   E P 
Sbjct: 181 AFMAPEVARGEKQGKESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPE 240

Query: 260 IPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAEDEEEEEESVVG 311
           +P   +L+ EA+ FL+ C  R+   R+TA  L+  PFL    + E   V G
Sbjct: 241 LP--CLLAEEAKDFLEKCLKREANERWTATQLLNHPFLTTKPDIEPVLVPG 272

The following BLAST results are available for this feature:

BLAST of MC09g0397 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SND6	3.2e-48	40.48	Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370...	[more]
O80888	7.7e-42	36.43	Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370...	[more]
Q9ZVP5	1.7e-33	36.33	Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=370...	[more]
P25390	4.4e-29	31.63	Serine/threonine-protein kinase SSK22 OS=Saccharomyces cerevisiae (strain ATCC 2...	[more]
Q54R82	1.1e-27	28.15	Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=...	[more]

BLAST of MC09g0397 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022154776.1	4.00e-239	100.00	mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia]	[more]
XP_008461430.1	4.39e-135	65.37	PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo]	[more]
XP_004136102.2	8.83e-135	65.00	mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus]	[more]
KAE8646420.1	3.01e-134	65.07	hypothetical protein Csa_015819 [Cucumis sativus]	[more]
KAG7014197.1	5.03e-134	66.06	Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosper...	[more]

BLAST of MC09g0397 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DPQ9	1.94e-239	100.00	mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX...	[more]
A0A0A0KBM3	1.50e-135	65.29	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G430...	[more]
A0A1S3CFZ6	2.12e-135	65.37	mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo OX=3656 GN...	[more]
A0A5D3CH22	1.41e-133	64.78	Mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo var. makuw...	[more]
A0A6J1GPL4	6.57e-132	66.25	mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=...	[more]

BLAST of MC09g0397 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G36950.1	8.1e-55	44.44	mitogen-activated protein kinase kinase kinase 21	[more]
AT5G67080.1	1.1e-51	41.47	mitogen-activated protein kinase kinase kinase 19	[more]
AT3G50310.1	2.3e-49	40.48	mitogen-activated protein kinase kinase kinase 20	[more]
AT4G26890.1	6.9e-46	39.30	mitogen-activated protein kinase kinase kinase 16	[more]
AT2G32510.1	5.4e-43	36.43	mitogen-activated protein kinase kinase kinase 17	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 22..297 e-value: 4.3E-54 score: 195.7
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 23..296 e-value: 3.6E-45 score: 154.4
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 22..297 score: 38.44944
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 111..328 e-value: 6.3E-47 score: 161.7
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 20..110 e-value: 4.5E-6 score: 28.3
None	No IPR available	PIRSR	PIRSR037993-2	PIRSR037993-2	coord: 22..307 e-value: 1.3E-9 score: 35.2
None	No IPR available	PIRSR	PIRSR000550-2	PIRSR000550-2	coord: 27..288 e-value: 2.4E-22 score: 77.0
None	No IPR available	PANTHER	PTHR48011	CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATED	coord: 19..309
None	No IPR available	PANTHER	PTHR48011:SF56	MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 3-LIKE	coord: 19..309
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 150..162
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 28..58
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 21..315

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC09g0397.1	MC09g0397.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004674	protein serine/threonine kinase activity
molecular_function	GO:0004672	protein kinase activity