MC08g_new0124 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g_new0124
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionVacuolar cation/proton exchanger
LocationMC08: 7198468 .. 7202619 (-)
RNA-Seq ExpressionMC08g_new0124
SyntenyMC08g_new0124
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGATCAATAGAGGAGAAAACAAATTACCTATCAGATGAAGAGATTCCATTCAGGTCATCACCAGTCGCGAAGAATGTATTGCATAGTTTTGATTTTGAGACGGCTCATGTGCGTTCTTCCCCAGATGCTTCAACATGGTGGAATATTAACAACATTCGTATTAGACCTCTCAAAAGTGCATATATAGTGTTGGTTAGAGCAAAGATCAACGTGTTGCTACCTTTTGGTCCTTTGGCAATTCTGCTACATTATTTAACTGGAAAACATGTAAGAAGAACTCATCATAATTTGTATTAAGCAGATGGCATGATTGGATTTGAAAAGAAAGAAATTTTTGAATGCTTCGTAAATTTTTCATGGAAGAGAAGCTCCTTGACATCAAAAGATTTTCTCTTTCTATTTGATTTGATAAGGATATAGCCTATGACCTCTCTGTGATGCTGTCATTTATAACTTCTATTGATCTACTCGTAAAAACTTGGATCTTCGAACATTCTAGTTTTCAGATATTTTTTGCTGCTTAGTATCGTGGCTTGTATGATTTGTCTCATGGTTCATAATTCCTTGATAGTCAGTTTTATATGTCAGGGATGGGTCTTCTTTTTCACTTTACTTGGAATTACACCTTTGGCAGAACGTCTTGGTTATGCAACTGAGTAAGATTTGGCTTCCCATATTTGCAGTTTAAGTTTTCGTATTATTTTCATTGTGCCACACTTACGTTTAAAAATACTTGTGATGCAGGCAACTTGCTTTCTACACTGGTCCAACAGGTATAAGTGCTACCATGATCATGCATTGCTTGCTTTGGTCATTTTTTTTATTCAAGTAGTTCCTCTTTTTGATGTCCTAACATAATAAGAAATTTAAATGGGTTTAGTTACTCTAAATGGTGGCTGTTTGGTTAGTTTCCTTAAACACTCAAATGTTCTAATGGGCTTTGCAGTTGGTGGTCTTTTGAACGCAACTTTTGGAAATGCAACCGAAATGATTATATCAATTTACGCATTAAAGAGTGGGATGATTAGGGTTGTTCAACAGTCATTACTGGGGTCAATCTTGTCAAATATGCTCCTAGTTCTTGGTTGCGCTTTCTTCACTGGTGGGATTATTCATCATAAGAAGGTGCAGACTTTTAATAAGGTACATGTTTTTGAGGAAACAATGCCATTATTTTGGTGATATTTTTACTCCCATTTTATTGGTTTACATTAAAACCTGACTATTTTGCAACAGGGTACTGCTGTTGTGAATTCAGGATTGTTGTTGATGGCTGTTATGGGAACAACTTTTCCGGCTGTGCTTCATTTCACCCACACAGAACTTCATTTTGGGGAGTCAGCATTGTCTCTTTCAAGATTTAGCAGTTGTATAATGTTAGTTGCATATGCAAGCTACCTTTTTTTTCAACTAAAAAGTCAGCATAATCTTTATGGTCCTCTTGATGAGGTGAGTCTATTTGGCTCTATGTTTGGATCTTGCTTTTGATTGTTGTTCTTTGGTTACCAAGCAATAGCTCCTCCAAATAACTTGGCTTCTTTTATCCATGACTTACAATAATAGGTCACAACTAGGAATCAAGATTTGCCACATCCATCGGAAATCTAAGTAAATTATTCTTGCACTATAAATTTGTTTTAGAAATGGCCATCGGGTGTTGTGCATCAAGAGCTCGAAGTGATGGCCATATAACAAAAATAAATGGGAATCTATTACCACCAAGAATTGCTAATGTTTGATATTACCATCCAGTTCTTTCTTTTGGGGGGGTGGTTTGGTGTGTGTTACTCTACCCATGTCCTTAGTTTGTCCTTTATATTGATAATACCAGATTTGTTTCTTAGGAAAAAAAAAGGAATTGCTAATGTGAGCATAGCTGAATTATCATACATGGCCTTAAGCACATGGGTCTTATATATATGGTGAACTTTAACTCGACTATGGAGTATATATTAGGAATGGGGATGCAATGGTGAGGCATGCACCTACTAAGGAGATGGTCTTTTTCAAAAAAGTAATAATAATAATATGAAGGCATCAAGGCAAGCATCTATTCATGTAAGATTTTTAGAGGTTGTTTGCATTGTTAGCATGTGCCCACGCTTCATAACACAGAATCAATTAAATGGCCAACAATATCTTTTCCAAGCAGGAGTTCAATTTTCAACCAGAGATTCTTTTAACTATAATGGTTTTTATATTTCTACAGGAAGTTGATGATGGAGATGCGGATGCTTCTGAAATGTTTGCCTGGGAAGCAATTGGATGGCTTGCTATCTTGACTGTATGGGTGTCTGTTTTATCTGGATACCTTGTGGATGCCATCCAGGTAAATTTTACTCCGATCAGATCATAGCTTATCTATGCACTTCTTTTCGCGATTATGTTGTATTTTGTGATTGTTTGATTTTCTTGTCAATCCATGGGCTGACTCTGATATTTGTTCTTGATTGCTCAAAGCTTAAGTGAAGCTTACAAACTTTAATCAATTGGTTTCTTATCCAGAAACAGTTGACAAAAGATAAAATAACAGCAATAAGATAAATAAGAACAAAAAGTATTTTTCAAATAGAAGTTAATTGAAATTAGTTTTCAAATTTATTCTTCAAAGCAGTTATCATAAAACACATCGAAAGACAGGCCTTAGCTTCATGGAATGGGCTTTGACTCCATTATTAGTTTGTGATGAATTCAAGAGTGATTTAGCTTTCAGCTTATAAGGTTAATGTCCTTGTGTCCTTTAATCTCTACTGGTGACCTATAGGTCAAAGGGGCAAGCTTGAAATTCACTTGTGAGAAGCTATGAGTTCTATTACTTACCTAAAATTTCGACATTGTATGAATTTCTTAACCCCGAAATTTGTAGGTTTAAACAGTAGTCCTATGGGACAAACCCATGGTTCTGTCTTCTGTGTTCTGTCCTCGTTTGCGTTGGGATTATCCATATTTTCTTTGTGTAATCTTCAAATTCTGAGCTTAGGCAGTAGCAATTATTTTGATCGTTGGACTTCCTCTCTCTGACTTTTCCCTCCTTTTTTCTTTCCCATTGAGTGTATAAAATCCTCGCAGTGTTCATCAAATTTAATTAGTTTGTGGGTGCTTTCTCAGGGAGCATCCGAGTCATTAAATTTGCCAGTGGCGTTTATTAGTGTAATTTTGTTGCCCATTGTTGGAAATGCTGCCGAACATGCAAGTGCAATCATGTTTGCCATGAAGGATAAGCTTGTGAGTACTTTGTGGATTTTTTCATTTTCTTCTAATCATCTCTTTTCTTTGGCTTGATTGACCTAAACGTTACTGCAGGACATAACCCTTGGAGTTGCTATTGGATCATCTACACAAATATCCATGTTTGTGGTAAGTCTTGAAATGGTAGTATCCCACATGATTAGTGAATTGTAGCAAAACTTTAGGTGAAATGGTAACATAATTTATATTAGTTTGAGAATTTTGACTTGAATTTTGTACATTATGTTGGATAGGAATTGATTTTATATTGGTAGCTGTAATTAAGTTTTCACTTAGATGACTTTGTGCCATTGATGATTGTAGATCCCGTTTTGCGTGGTCGTTGGATGGTGCATGGGAAAACCAATGGACTTGAACTTTAAACTCTTTGAGACAGCAACTCTGTTTATCACCGTGCTAGTGGTAGCCTTTATGTTGCAGGTTTGTTACCTTCTATCCATCCTCCTGTGATTAAATTCCTGGAATTTCATAGCTAGCTGTGCTGTATAAATAACTCTGTGGGGCTATTACTTTTGAATTTTTCAGTGCATTTTCATTTTCTTTTTATATCCGTGAGTGTCCAGGTTGATACGTTTTCATTTACAACCATTTAAAAAAAAAAGAGCCATTCAATCAAACATGAAGTTTAGTATAAATGAGAACGGGAATCAAAAGTTGGTTATCCACGAGAAGTTCTCTTGACAAGTTGAGTATAAATGCATCAGACATTCTAGGACTAGAACTATTGCTGACTTAATTTTGTATGTTCTGTATGATTAATCATTGTTCTCATCTGCTTGGCATTGGTCATATTTGCAGGAAGGGACATCAAATTATTTCAAAGGTCTAATGCTTATTCTATGCTACCTCATTGTGGCTGCCAGTTTTTTTGTACATGTTGACCCTAGCAATGGTGAGTAG

mRNA sequence

ATGGGATCAATAGAGGAGAAAACAAATTACCTATCAGATGAAGAGATTCCATTCAGGTCATCACCAGTCGCGAAGAATGTATTGCATAGTTTTGATTTTGAGACGGCTCATGTGCGTTCTTCCCCAGATGCTTCAACATGGTGGAATATTAACAACATTCGTATTAGACCTCTCAAAAGTGCATATATAGTGTTGGTTAGAGCAAAGATCAACGTGTTGCTACCTTTTGGTCCTTTGGCAATTCTGCTACATTATTTAACTGGAAAACATGGATGGGTCTTCTTTTTCACTTTACTTGGAATTACACCTTTGGCAGAACGTCTTGGTTATGCAACTGAGCAACTTGCTTTCTACACTGGTCCAACAGTTGGTGGTCTTTTGAACGCAACTTTTGGAAATGCAACCGAAATGATTATATCAATTTACGCATTAAAGAGTGGGATGATTAGGGTTGTTCAACAGTCATTACTGGGGTCAATCTTGTCAAATATGCTCCTAGTTCTTGGTTGCGCTTTCTTCACTGGTGGGATTATTCATCATAAGAAGGTGCAGACTTTTAATAAGGGTACTGCTGTTGTGAATTCAGGATTGTTGTTGATGGCTGTTATGGGAACAACTTTTCCGGCTGTGCTTCATTTCACCCACACAGAACTTCATTTTGGGGAGTCAGCATTGTCTCTTTCAAGATTTAGCAGTTGTATAATGTTAGTTGCATATGCAAGCTACCTTTTTTTTCAACTAAAAAGTCAGCATAATCTTTATGGTCCTCTTGATGAGGAAGTTGATGATGGAGATGCGGATGCTTCTGAAATGTTTGCCTGGGAAGCAATTGGATGGCTTGCTATCTTGACTGTATGGGTGTCTGTTTTATCTGGATACCTTGTGGATGCCATCCAGGGAGCATCCGAGTCATTAAATTTGCCAGTGGCGTTTATTAGTGTAATTTTGTTGCCCATTGTTGGAAATGCTGCCGAACATGCAAGTGCAATCATGTTTGCCATGAAGGATAAGCTTGACATAACCCTTGGAGTTGCTATTGGATCATCTACACAAATATCCATGTTTGTGATCCCGTTTTGCGTGGTCGTTGGATGGTGCATGGGAAAACCAATGGACTTGAACTTTAAACTCTTTGAGACAGCAACTCTGTTTATCACCGTGCTAGTGGTAGCCTTTATGTTGCAGGAAGGGACATCAAATTATTTCAAAGGTCTAATGCTTATTCTATGCTACCTCATTGTGGCTGCCAGTTTTTTTGTACATGTTGACCCTAGCAATGGTGAGTAG

Coding sequence (CDS)

ATGGGATCAATAGAGGAGAAAACAAATTACCTATCAGATGAAGAGATTCCATTCAGGTCATCACCAGTCGCGAAGAATGTATTGCATAGTTTTGATTTTGAGACGGCTCATGTGCGTTCTTCCCCAGATGCTTCAACATGGTGGAATATTAACAACATTCGTATTAGACCTCTCAAAAGTGCATATATAGTGTTGGTTAGAGCAAAGATCAACGTGTTGCTACCTTTTGGTCCTTTGGCAATTCTGCTACATTATTTAACTGGAAAACATGGATGGGTCTTCTTTTTCACTTTACTTGGAATTACACCTTTGGCAGAACGTCTTGGTTATGCAACTGAGCAACTTGCTTTCTACACTGGTCCAACAGTTGGTGGTCTTTTGAACGCAACTTTTGGAAATGCAACCGAAATGATTATATCAATTTACGCATTAAAGAGTGGGATGATTAGGGTTGTTCAACAGTCATTACTGGGGTCAATCTTGTCAAATATGCTCCTAGTTCTTGGTTGCGCTTTCTTCACTGGTGGGATTATTCATCATAAGAAGGTGCAGACTTTTAATAAGGGTACTGCTGTTGTGAATTCAGGATTGTTGTTGATGGCTGTTATGGGAACAACTTTTCCGGCTGTGCTTCATTTCACCCACACAGAACTTCATTTTGGGGAGTCAGCATTGTCTCTTTCAAGATTTAGCAGTTGTATAATGTTAGTTGCATATGCAAGCTACCTTTTTTTTCAACTAAAAAGTCAGCATAATCTTTATGGTCCTCTTGATGAGGAAGTTGATGATGGAGATGCGGATGCTTCTGAAATGTTTGCCTGGGAAGCAATTGGATGGCTTGCTATCTTGACTGTATGGGTGTCTGTTTTATCTGGATACCTTGTGGATGCCATCCAGGGAGCATCCGAGTCATTAAATTTGCCAGTGGCGTTTATTAGTGTAATTTTGTTGCCCATTGTTGGAAATGCTGCCGAACATGCAAGTGCAATCATGTTTGCCATGAAGGATAAGCTTGACATAACCCTTGGAGTTGCTATTGGATCATCTACACAAATATCCATGTTTGTGATCCCGTTTTGCGTGGTCGTTGGATGGTGCATGGGAAAACCAATGGACTTGAACTTTAAACTCTTTGAGACAGCAACTCTGTTTATCACCGTGCTAGTGGTAGCCTTTATGTTGCAGGAAGGGACATCAAATTATTTCAAAGGTCTAATGCTTATTCTATGCTACCTCATTGTGGCTGCCAGTTTTTTTGTACATGTTGACCCTAGCAATGGTGAGTAG

Protein sequence

MGSIEEKTNYLSDEEIPFRSSPVAKNVLHSFDFETAHVRSSPDASTWWNINNIRIRPLKSAYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTGPTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHHKKVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHFGESALSLSRFSSCIMLVAYASYLFFQLKSQHNLYGPLDEEVDDGDADASEMFAWEAIGWLAILTVWVSVLSGYLVDAIQGASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFCVVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFVHVDPSNGE
Homology
BLAST of MC08g_new0124 vs. ExPASy Swiss-Prot
Match: Q5KQN0 (Vacuolar cation/proton exchanger 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CAX2 PE=2 SV=2)

HSP 1 Score: 588.6 bits (1516), Expect = 5.8e-167
Identity = 308/414 (74.40%), Postives = 352/414 (85.02%), Query Frame = 0

Query: 17  PFRSSPVAKNVLHSFDFETAHVRSSPDASTWWNINNIRIRPLKSAYIVLVRAKINVLLPF 76
           P  S   A   + + DFE  H+ S    +   +  +   R L S  +V+++AKINVLLPF
Sbjct: 22  PSPSPMTAAGKMQALDFE--HIGSLAAVAESLSTGSKWRRALTSVRVVILQAKINVLLPF 81

Query: 77  GPLAILLHYLTGKH-GWVFFFTLLGITPLAERLGYATEQLAFYTGPTVGGLLNATFGNAT 136
           GPLA++LHYL+  H GWVF F+L+GITPLAERLGYATEQLA YTGPT+GGLLNATFGNAT
Sbjct: 82  GPLAVMLHYLSANHQGWVFLFSLIGITPLAERLGYATEQLALYTGPTIGGLLNATFGNAT 141

Query: 137 EMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHHKKVQTFNKGTAVVNS 196
           EMIIS+YALK+GMIRVVQQSLLGSILSNMLLVLGCAFF GG++H  + Q FNK +AVVNS
Sbjct: 142 EMIISLYALKNGMIRVVQQSLLGSILSNMLLVLGCAFFAGGLVHPSRDQVFNKASAVVNS 201

Query: 197 GLLLMAVMGTTFPAVLHFTHTELHFGESALSLSRFSSCIMLVAYASYLFFQLKSQHNLYG 256
           GLLLMAV+G  FPAVLHFTH+E+ +G+S +SLSRFSSCIMLVAYASYLFFQLKSQ +LY 
Sbjct: 202 GLLLMAVLGLMFPAVLHFTHSEVQYGKSEVSLSRFSSCIMLVAYASYLFFQLKSQRSLYS 261

Query: 257 PL---DEEVDDGDADASEMFAWEAIGWLAILTVWVSVLSGYLVDAIQGASESLNLPVAFI 316
           P+   +EEV + + +  E+   EAI WL +LT+W+S+LSGYLVDAIQGASESLN+PVAFI
Sbjct: 262 PIGEQEEEVTEDEEEEKEITQGEAICWLFVLTIWISILSGYLVDAIQGASESLNMPVAFI 321

Query: 317 SVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFCVVVGWCMGKPMD 376
           SVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFCVV+GW MG+ MD
Sbjct: 322 SVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFCVVIGWIMGQQMD 381

Query: 377 LNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFVHVDPSN 427
           LNF+LFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFVHVDP +
Sbjct: 382 LNFQLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFVHVDPDS 433

BLAST of MC08g_new0124 vs. ExPASy Swiss-Prot
Match: Q6K1C4 (Vacuolar cation/proton exchanger 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CAX3 PE=2 SV=2)

HSP 1 Score: 567.8 bits (1462), Expect = 1.1e-160
Identity = 293/386 (75.91%), Postives = 334/386 (86.53%), Query Frame = 0

Query: 48  WNINNIRIRPLKSAYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAER 107
           WN+       L+S  IV+  +K+N+LLPFGP +I+LHY T +HG VF F++LGITPLAER
Sbjct: 31  WNLQRFVESALRSIRIVIFTSKLNLLLPFGPASIILHYTTSRHGLVFLFSMLGITPLAER 90

Query: 108 LGYATEQLAFYTGPTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLV 167
           LGYATEQLA YTGPTVGGLLNATFGNATEMII+IYALK+GMIRVVQQSLLGSILSNMLLV
Sbjct: 91  LGYATEQLAIYTGPTVGGLLNATFGNATEMIIAIYALKNGMIRVVQQSLLGSILSNMLLV 150

Query: 168 LGCAFFTGGIIHHKKVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHFGESALSL 227
           +GCAFF GGI+H  K Q F+K TAVVNSGLLLMAVMG  FPAVLHFTH+E+  G S +SL
Sbjct: 151 MGCAFFAGGIVHRNKDQVFSKATAVVNSGLLLMAVMGLMFPAVLHFTHSEVRQGASEVSL 210

Query: 228 SRFSSCIMLVAYASYLFFQLKSQHNLYGPL-DEEVDDGDA----DASEMFAWEAIGWLAI 287
           SRFSSCIMLVAYASYL+FQL  ++N Y P+  EE+ + DA    + SE+  WE+I WLA+
Sbjct: 211 SRFSSCIMLVAYASYLYFQLSGRNNAYSPIGSEEMPNEDAAEEDEESEIGMWESIAWLAM 270

Query: 288 LTVWVSVLSGYLVDAIQGASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITL 347
           LT+WVS+LS YLV+AI+GAS+SLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITL
Sbjct: 271 LTLWVSILSEYLVNAIEGASDSLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITL 330

Query: 348 GVAIGSSTQISMFVIPFCVVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYF 407
           GVAIGSSTQISMFVIPFCVV+GW MG+ MDLNF+LFETATLFITVLVVAFMLQ+G +NY 
Sbjct: 331 GVAIGSSTQISMFVIPFCVVIGWMMGQKMDLNFQLFETATLFITVLVVAFMLQDGVANYL 390

Query: 408 KGLMLILCYLIVAASFFVHVDPSNGE 429
           KGLMLILCYLIVAASFFVHVDP + +
Sbjct: 391 KGLMLILCYLIVAASFFVHVDPQSSD 416

BLAST of MC08g_new0124 vs. ExPASy Swiss-Prot
Match: Q39254 (Vacuolar cation/proton exchanger 2 OS=Arabidopsis thaliana OX=3702 GN=CAX2 PE=1 SV=2)

HSP 1 Score: 542.7 bits (1397), Expect = 3.6e-153
Identity = 279/372 (75.00%), Postives = 318/372 (85.48%), Query Frame = 0

Query: 58  LKSAYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAF 117
           L S  IV+   K+N+LLPFGPLAIL+HY+    GWVF  TL+GITPLAERLGYATEQLA 
Sbjct: 65  LNSIKIVIFCNKLNLLLPFGPLAILVHYMIDSKGWVFLLTLVGITPLAERLGYATEQLAC 124

Query: 118 YTGPTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGI 177
           YTGPTVGGLLNATFGN TE+IISI+ALK+GMIRVVQ +LLGSILSNMLLVLGCAFF GG+
Sbjct: 125 YTGPTVGGLLNATFGNVTELIISIFALKNGMIRVVQLTLLGSILSNMLLVLGCAFFCGGL 184

Query: 178 IHHKKVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHFGESALSLSRFSSCIMLV 237
           + ++K Q F+KG A VNSGLLLMAVMG  FPAVLH+TH+E+H G S L+LSRFSSCIML+
Sbjct: 185 VFYQKDQVFDKGIATVNSGLLLMAVMGILFPAVLHYTHSEVHAGSSELALSRFSSCIMLI 244

Query: 238 AYASYLFFQLKSQHNLYGPLDEEVDDGDADAS-----EMFAWEAIGWLAILTVWVSVLSG 297
           AYA+YLFFQLKSQ N Y PLDEE +  +  ++     E+  WEAI WL+ILT WVS+LSG
Sbjct: 245 AYAAYLFFQLKSQSNSYSPLDEESNQNEETSAEDEDPEISKWEAIIWLSILTAWVSLLSG 304

Query: 298 YLVDAIQGASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQI 357
           YLVDAI+GAS S N+P+AFIS ILLPIVGNAAEHA AIMFAMKDKLD++LGVAIGSS QI
Sbjct: 305 YLVDAIEGASVSWNIPIAFISTILLPIVGNAAEHAGAIMFAMKDKLDLSLGVAIGSSIQI 364

Query: 358 SMFVIPFCVVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYL 417
           SMF +PFCVV+GW MG+ MDLNF+LFETA LFITV+VVAF LQEG+SNYFKGLMLILCYL
Sbjct: 365 SMFAVPFCVVIGWMMGQQMDLNFQLFETAMLFITVIVVAFFLQEGSSNYFKGLMLILCYL 424

Query: 418 IVAASFFVHVDP 425
           IVAASFFVH DP
Sbjct: 425 IVAASFFVHEDP 436

BLAST of MC08g_new0124 vs. ExPASy Swiss-Prot
Match: Q8L783 (Vacuolar cation/proton exchanger 5 OS=Arabidopsis thaliana OX=3702 GN=CAX5 PE=2 SV=1)

HSP 1 Score: 542.7 bits (1397), Expect = 3.6e-153
Identity = 282/372 (75.81%), Postives = 318/372 (85.48%), Query Frame = 0

Query: 58  LKSAYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAF 117
           L+S  IV++  K+N+LLPFGPLAILLHYLT   GW+F  +L+GITPLAERLGYATEQLA 
Sbjct: 65  LQSFKIVILSNKLNLLLPFGPLAILLHYLTDNKGWIFLLSLVGITPLAERLGYATEQLAC 124

Query: 118 YTGPTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGI 177
           YTG TVGGLLNATFGN TE+IISI+ALKSGMIRVVQ +LLGSILSNMLLVLGCAFF GG+
Sbjct: 125 YTGSTVGGLLNATFGNVTELIISIFALKSGMIRVVQLTLLGSILSNMLLVLGCAFFCGGL 184

Query: 178 IHHKKVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHFGESALSLSRFSSCIMLV 237
           +  +K Q F+KG AVVNSGLLLMAVMG  FPAVLH+TH+E+H G S L+LSRFSSCIMLV
Sbjct: 185 VFSQKEQVFDKGNAVVNSGLLLMAVMGLLFPAVLHYTHSEVHAGSSELALSRFSSCIMLV 244

Query: 238 AYASYLFFQLKSQHNLYGPLDEEVDDG-----DADASEMFAWEAIGWLAILTVWVSVLSG 297
           AYA+YLFFQLKSQ + Y PL EE +       D +  E+  WEAI WL+ILT WVS+LSG
Sbjct: 245 AYAAYLFFQLKSQPSSYTPLTEETNQNEETSDDDEDPEISKWEAIIWLSILTAWVSLLSG 304

Query: 298 YLVDAIQGASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQI 357
           YLVDAI+GAS S  +P++FISVILLPIVGNAAEHA AIMFAMKDKLD++LGVAIGSS QI
Sbjct: 305 YLVDAIEGASVSWKIPISFISVILLPIVGNAAEHAGAIMFAMKDKLDLSLGVAIGSSIQI 364

Query: 358 SMFVIPFCVVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYL 417
           SMF +PFCVV+GW MG  MDLNF+LFETATLFITV+VVAF LQEGTSNYFKGLMLILCYL
Sbjct: 365 SMFAVPFCVVIGWMMGAQMDLNFQLFETATLFITVIVVAFFLQEGTSNYFKGLMLILCYL 424

Query: 418 IVAASFFVHVDP 425
           IVAASFFVH DP
Sbjct: 425 IVAASFFVHEDP 436

BLAST of MC08g_new0124 vs. ExPASy Swiss-Prot
Match: Q9LFZ8 (Putative vacuolar cation/proton exchanger 6 OS=Arabidopsis thaliana OX=3702 GN=CAX6 PE=3 SV=3)

HSP 1 Score: 499.6 bits (1285), Expect = 3.5e-140
Identity = 265/374 (70.86%), Postives = 305/374 (81.55%), Query Frame = 0

Query: 58  LKSAYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAF 117
           ++S  IV++  K+N+LLPFGPLAIL+HYLT   GW F  +L+GITPLAERLGYATEQL+ 
Sbjct: 76  VQSFKIVILSNKLNLLLPFGPLAILVHYLTDNKGWFFLLSLVGITPLAERLGYATEQLSC 135

Query: 118 YTGPTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGI 177
           YTG TVGGLLNATFGN  E+IISI ALK+GMIRVVQ +LLGSILSN+LLVLGCAFF GG+
Sbjct: 136 YTGATVGGLLNATFGNVIELIISIIALKNGMIRVVQLTLLGSILSNILLVLGCAFFCGGL 195

Query: 178 IHHKKVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHFGESALSLSRFSSCIMLV 237
           +   K Q F+K  AVV+SG+LLMAVMG  FP  LH+TH+E+H G S L+LSRF SCIMLV
Sbjct: 196 VFPGKDQVFDKRNAVVSSGMLLMAVMGLLFPTFLHYTHSEVHAGSSELALSRFISCIMLV 255

Query: 238 AYASYLFFQLKSQHNLYGP---LDEEVDDGDADASEMFAWEAIGWLAILTVWVSVLSGYL 297
           AYA+YLFFQLKSQ + Y      +EE  + D D  E+  WEAI WL+I T WVS+LSGYL
Sbjct: 256 AYAAYLFFQLKSQPSFYTEKTNQNEETSNDDED-PEISKWEAIIWLSIFTAWVSLLSGYL 315

Query: 298 VDAIQGASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISM 357
           VDAI+G S S  +P++FISVILLPIVGNAAEHA AIMFAMKDKLD++LGVAIGSS QISM
Sbjct: 316 VDAIEGTSVSWKIPISFISVILLPIVGNAAEHAGAIMFAMKDKLDLSLGVAIGSSIQISM 375

Query: 358 FVIPFCVVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQ-EGTSNYFKGLMLILCYLI 417
           F +PFCVV+GW MG  MDLN +LFETATL ITV+VVAF LQ EGTSNYFK LMLILCYLI
Sbjct: 376 FAVPFCVVIGWMMGAQMDLNLQLFETATLLITVIVVAFFLQLEGTSNYFKRLMLILCYLI 435

Query: 418 VAASFFVHVDPSNG 428
           VAASFFVH DP  G
Sbjct: 436 VAASFFVHEDPHQG 448

BLAST of MC08g_new0124 vs. NCBI nr
Match: XP_022152617.1 (vacuolar cation/proton exchanger 3-like [Momordica charantia])

HSP 1 Score: 833 bits (2153), Expect = 2.07e-304
Identity = 426/428 (99.53%), Postives = 427/428 (99.77%), Query Frame = 0

Query: 1   MGSIEEKTNYLSDEEIPFRSSPVAKNVLHSFDFETAHVRSSPDASTWWNINNIRIRPLKS 60
           MGSIEEKTNYLSDEEIPFRSSPVAKNVLHSFDFETAHVRSSPDASTWWNINNIRIRPLKS
Sbjct: 1   MGSIEEKTNYLSDEEIPFRSSPVAKNVLHSFDFETAHVRSSPDASTWWNINNIRIRPLKS 60

Query: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG 120
           AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG
Sbjct: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG 120

Query: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH 180
           PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH
Sbjct: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH 180

Query: 181 KKVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHFGESALSLSRFSSCIMLVAYA 240
           KKVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHFGESALSLSRFSSCIMLVAYA
Sbjct: 181 KKVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHFGESALSLSRFSSCIMLVAYA 240

Query: 241 SYLFFQLKSQHNLYGPLDEEVDDGDADASEMFAWEAIGWLAILTVWVSVLSGYLVDAIQG 300
           SYLFFQLKSQHNLYGPLDEEVDDGDADASEMFAWEAIGWLAILTVWVSVLSGYLVDAIQG
Sbjct: 241 SYLFFQLKSQHNLYGPLDEEVDDGDADASEMFAWEAIGWLAILTVWVSVLSGYLVDAIQG 300

Query: 301 ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC 360
           ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC
Sbjct: 301 ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC 360

Query: 361 VVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420
           VVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV
Sbjct: 361 VVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420

Query: 421 HVDPSNGE 428
           HVDPSN +
Sbjct: 421 HVDPSNDD 428

BLAST of MC08g_new0124 vs. NCBI nr
Match: XP_008463979.1 (PREDICTED: vacuolar cation/proton exchanger 3-like [Cucumis melo] >XP_008463980.1 PREDICTED: vacuolar cation/proton exchanger 3-like [Cucumis melo] >XP_008463981.1 PREDICTED: vacuolar cation/proton exchanger 3-like [Cucumis melo])

HSP 1 Score: 783 bits (2023), Expect = 1.29e-284
Identity = 404/428 (94.39%), Postives = 411/428 (96.03%), Query Frame = 0

Query: 1   MGSIEEKTNYLSDEEIPFRSSPVAKNVLHSFDFETAHVRSSPDASTWWNINNIRIRPLKS 60
           MGS EEKTNYLSDEEIP +SSPVAKNVLHSFD ETA VRSSPD + WW  NNIRIRPL+S
Sbjct: 1   MGSTEEKTNYLSDEEIPLKSSPVAKNVLHSFDCETAAVRSSPD-TRWWKTNNIRIRPLRS 60

Query: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG 120
           AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG
Sbjct: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG 120

Query: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH 180
           PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH
Sbjct: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH 180

Query: 181 KKVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHFGESALSLSRFSSCIMLVAYA 240
           KKVQ FNKG AVVNSGLLLMAVMGTTFPAVLHFTHTELH GESALSLSRFSSCIML+AYA
Sbjct: 181 KKVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA 240

Query: 241 SYLFFQLKSQHNLYGPLDEEVDDGDADASEMFAWEAIGWLAILTVWVSVLSGYLVDAIQG 300
           SYLFFQLKSQHNLYGPLDEEVD    D SE+FAWEAIGWLAILTVWVS+LSGYLVDAIQG
Sbjct: 241 SYLFFQLKSQHNLYGPLDEEVDGEVEDDSEIFAWEAIGWLAILTVWVSILSGYLVDAIQG 300

Query: 301 ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC 360
           ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC
Sbjct: 301 ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC 360

Query: 361 VVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420
           VVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV
Sbjct: 361 VVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420

Query: 421 HVDPSNGE 428
           HVDPSN +
Sbjct: 421 HVDPSNDD 427

BLAST of MC08g_new0124 vs. NCBI nr
Match: NP_001267651.1 (vacuolar cation/proton exchanger 2-like [Cucumis sativus] >XP_011650230.1 vacuolar cation/proton exchanger 2-like isoform X1 [Cucumis sativus] >XP_011650232.1 vacuolar cation/proton exchanger 2-like isoform X1 [Cucumis sativus] >XP_011650233.1 vacuolar cation/proton exchanger 2-like isoform X1 [Cucumis sativus] >XP_031737456.1 vacuolar cation/proton exchanger 2-like isoform X1 [Cucumis sativus] >ACU82368.1 cation exchanger 2 [Cucumis sativus] >KGN58485.1 hypothetical protein Csa_001856 [Cucumis sativus])

HSP 1 Score: 777 bits (2007), Expect = 3.41e-282
Identity = 401/428 (93.69%), Postives = 410/428 (95.79%), Query Frame = 0

Query: 1   MGSIEEKTNYLSDEEIPFRSSPVAKNVLHSFDFETAHVRSSPDASTWWNINNIRIRPLKS 60
           MGS EEKTNYLSDEEIP +SSPVAKNVLHSFD ETA VRSSPD + WW  +NIRIRPL+S
Sbjct: 1   MGSTEEKTNYLSDEEIPLKSSPVAKNVLHSFDCETAAVRSSPD-TRWWKTSNIRIRPLRS 60

Query: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG 120
           AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG
Sbjct: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG 120

Query: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH 180
           PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH
Sbjct: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH 180

Query: 181 KKVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHFGESALSLSRFSSCIMLVAYA 240
            KVQ FNKG AVVNSGLLLMAVMGTTFPAVLHFTHTELH GESALSLSRFSSCIML+AYA
Sbjct: 181 TKVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHLGESALSLSRFSSCIMLIAYA 240

Query: 241 SYLFFQLKSQHNLYGPLDEEVDDGDADASEMFAWEAIGWLAILTVWVSVLSGYLVDAIQG 300
           SYLFFQLKSQH+LYGPLDEEVD    D SE++AWEAIGWLAILTVWVSVLSGYLVDAIQG
Sbjct: 241 SYLFFQLKSQHDLYGPLDEEVDGEVEDDSEIYAWEAIGWLAILTVWVSVLSGYLVDAIQG 300

Query: 301 ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC 360
           ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC
Sbjct: 301 ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC 360

Query: 361 VVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420
           VVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV
Sbjct: 361 VVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420

Query: 421 HVDPSNGE 428
           HVDPSN +
Sbjct: 421 HVDPSNDD 427

BLAST of MC08g_new0124 vs. NCBI nr
Match: XP_038902338.1 (vacuolar cation/proton exchanger 3-like [Benincasa hispida])

HSP 1 Score: 772 bits (1994), Expect = 3.79e-280
Identity = 400/430 (93.02%), Postives = 409/430 (95.12%), Query Frame = 0

Query: 1   MGSIEEKTNYLSDEEIPFRSSPVAKNVLHSFDFETAHVRSSPDASTWWNINNIRIRPLKS 60
           MGS EEKTNYLSDEEI  +SSPVAKNVLHSFD ETA VRSSPD   WW  NNIRIRPL+S
Sbjct: 1   MGSTEEKTNYLSDEEILLKSSPVAKNVLHSFDCETAPVRSSPDDKRWWKTNNIRIRPLRS 60

Query: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG 120
           AYIVLVRAKINVLLPFGPLAILLHYLTGKHG VFFFTLLGITPLAERLGYATEQLAFYTG
Sbjct: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLAFYTG 120

Query: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH 180
           PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGI+HH
Sbjct: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH 180

Query: 181 KKVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHFGESALSLSRFSSCIMLVAYA 240
           KKVQ FNKG AVVNSGLLLMAVMGTTFPAVLHFTHTELH GESALSLSRFSSCIMLVAYA
Sbjct: 181 KKVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLVAYA 240

Query: 241 SYLFFQLKSQHNLYGPLDEEVD--DGDADASEMFAWEAIGWLAILTVWVSVLSGYLVDAI 300
           SYLFFQLKSQHNLYGPLD EV+  D D D SE++AWEAIGWLAILTVWVS+LSGYLVDAI
Sbjct: 241 SYLFFQLKSQHNLYGPLDGEVEVEDEDEDDSEIYAWEAIGWLAILTVWVSILSGYLVDAI 300

Query: 301 QGASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIP 360
           QGASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIP
Sbjct: 301 QGASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIP 360

Query: 361 FCVVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASF 420
           FCVVVGWCMG+PMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASF
Sbjct: 361 FCVVVGWCMGEPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASF 420

Query: 421 FVHVDPSNGE 428
           FVHVDPSN +
Sbjct: 421 FVHVDPSNDD 430

BLAST of MC08g_new0124 vs. NCBI nr
Match: XP_023526825.1 (vacuolar cation/proton exchanger 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 757 bits (1955), Expect = 2.96e-274
Identity = 389/428 (90.89%), Postives = 403/428 (94.16%), Query Frame = 0

Query: 1   MGSIEEKTNYLSDEEIPFRSSPVAKNVLHSFDFETAHVRSSPDASTWWNINNIRIRPLKS 60
           MGS EEKTNYLSDE  P +S PVAKNV HSFD ET  VRSSPD + WW  NNIRIRPL+S
Sbjct: 1   MGSTEEKTNYLSDEGTPLKSPPVAKNVSHSFDCETGPVRSSPDVTRWWKTNNIRIRPLRS 60

Query: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG 120
           AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGI PLAERLGYATEQLAFYTG
Sbjct: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGIAPLAERLGYATEQLAFYTG 120

Query: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH 180
           PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGI+HH
Sbjct: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH 180

Query: 181 KKVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHFGESALSLSRFSSCIMLVAYA 240
           K VQ FNKG+AVVNSGLLLMAVMGTTFPAVLHFTHTELH GESALSLSRFSSCIML+AYA
Sbjct: 181 K-VQVFNKGSAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA 240

Query: 241 SYLFFQLKSQHNLYGPLDEEVDDGDADASEMFAWEAIGWLAILTVWVSVLSGYLVDAIQG 300
           SYLFFQLKSQ +LYGPLDE V+D   D SE++AWEAIGWLAILTVWVS+LSGYLVDAIQG
Sbjct: 241 SYLFFQLKSQQDLYGPLDEVVEDEVEDDSEIYAWEAIGWLAILTVWVSILSGYLVDAIQG 300

Query: 301 ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC 360
           ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC
Sbjct: 301 ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC 360

Query: 361 VVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420
           VVVGWCMG+PMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV
Sbjct: 361 VVVGWCMGEPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420

Query: 421 HVDPSNGE 428
           H+DP+N  
Sbjct: 421 HIDPTNDN 427

BLAST of MC08g_new0124 vs. ExPASy TrEMBL
Match: A0A6J1DGR6 (Vacuolar cation/proton exchanger OS=Momordica charantia OX=3673 GN=LOC111020296 PE=3 SV=1)

HSP 1 Score: 833 bits (2153), Expect = 1.00e-304
Identity = 426/428 (99.53%), Postives = 427/428 (99.77%), Query Frame = 0

Query: 1   MGSIEEKTNYLSDEEIPFRSSPVAKNVLHSFDFETAHVRSSPDASTWWNINNIRIRPLKS 60
           MGSIEEKTNYLSDEEIPFRSSPVAKNVLHSFDFETAHVRSSPDASTWWNINNIRIRPLKS
Sbjct: 1   MGSIEEKTNYLSDEEIPFRSSPVAKNVLHSFDFETAHVRSSPDASTWWNINNIRIRPLKS 60

Query: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG 120
           AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG
Sbjct: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG 120

Query: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH 180
           PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH
Sbjct: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH 180

Query: 181 KKVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHFGESALSLSRFSSCIMLVAYA 240
           KKVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHFGESALSLSRFSSCIMLVAYA
Sbjct: 181 KKVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHFGESALSLSRFSSCIMLVAYA 240

Query: 241 SYLFFQLKSQHNLYGPLDEEVDDGDADASEMFAWEAIGWLAILTVWVSVLSGYLVDAIQG 300
           SYLFFQLKSQHNLYGPLDEEVDDGDADASEMFAWEAIGWLAILTVWVSVLSGYLVDAIQG
Sbjct: 241 SYLFFQLKSQHNLYGPLDEEVDDGDADASEMFAWEAIGWLAILTVWVSVLSGYLVDAIQG 300

Query: 301 ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC 360
           ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC
Sbjct: 301 ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC 360

Query: 361 VVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420
           VVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV
Sbjct: 361 VVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420

Query: 421 HVDPSNGE 428
           HVDPSN +
Sbjct: 421 HVDPSNDD 428

BLAST of MC08g_new0124 vs. ExPASy TrEMBL
Match: A0A1S3CKH8 (Vacuolar cation/proton exchanger OS=Cucumis melo OX=3656 GN=LOC103501973 PE=3 SV=1)

HSP 1 Score: 783 bits (2023), Expect = 6.26e-285
Identity = 404/428 (94.39%), Postives = 411/428 (96.03%), Query Frame = 0

Query: 1   MGSIEEKTNYLSDEEIPFRSSPVAKNVLHSFDFETAHVRSSPDASTWWNINNIRIRPLKS 60
           MGS EEKTNYLSDEEIP +SSPVAKNVLHSFD ETA VRSSPD + WW  NNIRIRPL+S
Sbjct: 1   MGSTEEKTNYLSDEEIPLKSSPVAKNVLHSFDCETAAVRSSPD-TRWWKTNNIRIRPLRS 60

Query: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG 120
           AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG
Sbjct: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG 120

Query: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH 180
           PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH
Sbjct: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH 180

Query: 181 KKVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHFGESALSLSRFSSCIMLVAYA 240
           KKVQ FNKG AVVNSGLLLMAVMGTTFPAVLHFTHTELH GESALSLSRFSSCIML+AYA
Sbjct: 181 KKVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA 240

Query: 241 SYLFFQLKSQHNLYGPLDEEVDDGDADASEMFAWEAIGWLAILTVWVSVLSGYLVDAIQG 300
           SYLFFQLKSQHNLYGPLDEEVD    D SE+FAWEAIGWLAILTVWVS+LSGYLVDAIQG
Sbjct: 241 SYLFFQLKSQHNLYGPLDEEVDGEVEDDSEIFAWEAIGWLAILTVWVSILSGYLVDAIQG 300

Query: 301 ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC 360
           ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC
Sbjct: 301 ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC 360

Query: 361 VVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420
           VVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV
Sbjct: 361 VVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420

Query: 421 HVDPSNGE 428
           HVDPSN +
Sbjct: 421 HVDPSNDD 427

BLAST of MC08g_new0124 vs. ExPASy TrEMBL
Match: C8CBQ5 (Vacuolar cation/proton exchanger OS=Cucumis sativus OX=3659 GN=CAX2 PE=2 SV=1)

HSP 1 Score: 777 bits (2007), Expect = 1.65e-282
Identity = 401/428 (93.69%), Postives = 410/428 (95.79%), Query Frame = 0

Query: 1   MGSIEEKTNYLSDEEIPFRSSPVAKNVLHSFDFETAHVRSSPDASTWWNINNIRIRPLKS 60
           MGS EEKTNYLSDEEIP +SSPVAKNVLHSFD ETA VRSSPD + WW  +NIRIRPL+S
Sbjct: 1   MGSTEEKTNYLSDEEIPLKSSPVAKNVLHSFDCETAAVRSSPD-TRWWKTSNIRIRPLRS 60

Query: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG 120
           AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG
Sbjct: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG 120

Query: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH 180
           PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH
Sbjct: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH 180

Query: 181 KKVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHFGESALSLSRFSSCIMLVAYA 240
            KVQ FNKG AVVNSGLLLMAVMGTTFPAVLHFTHTELH GESALSLSRFSSCIML+AYA
Sbjct: 181 TKVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHLGESALSLSRFSSCIMLIAYA 240

Query: 241 SYLFFQLKSQHNLYGPLDEEVDDGDADASEMFAWEAIGWLAILTVWVSVLSGYLVDAIQG 300
           SYLFFQLKSQH+LYGPLDEEVD    D SE++AWEAIGWLAILTVWVSVLSGYLVDAIQG
Sbjct: 241 SYLFFQLKSQHDLYGPLDEEVDGEVEDDSEIYAWEAIGWLAILTVWVSVLSGYLVDAIQG 300

Query: 301 ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC 360
           ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC
Sbjct: 301 ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC 360

Query: 361 VVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420
           VVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV
Sbjct: 361 VVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420

Query: 421 HVDPSNGE 428
           HVDPSN +
Sbjct: 421 HVDPSNDD 427

BLAST of MC08g_new0124 vs. ExPASy TrEMBL
Match: A0A6J1IRU5 (Vacuolar cation/proton exchanger OS=Cucurbita maxima OX=3661 GN=LOC111479941 PE=3 SV=1)

HSP 1 Score: 755 bits (1950), Expect = 8.29e-274
Identity = 388/428 (90.65%), Postives = 403/428 (94.16%), Query Frame = 0

Query: 1   MGSIEEKTNYLSDEEIPFRSSPVAKNVLHSFDFETAHVRSSPDASTWWNINNIRIRPLKS 60
           MGS EEKTNYLSDE  P +SSPVAKNV HSFD ET  VRSSPD + WW  NNIRIRPL+S
Sbjct: 1   MGSTEEKTNYLSDEGTPLKSSPVAKNVSHSFDCETGPVRSSPDVTRWWKTNNIRIRPLRS 60

Query: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG 120
           AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGI PLAERLGYATEQLAFYTG
Sbjct: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGIAPLAERLGYATEQLAFYTG 120

Query: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH 180
           PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGI+HH
Sbjct: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH 180

Query: 181 KKVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHFGESALSLSRFSSCIMLVAYA 240
           K VQ FNKG+AVVNSGLLLMAVMGTTFPAVLHFTHTELH GESALSLSRFSSCIML+AYA
Sbjct: 181 K-VQVFNKGSAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA 240

Query: 241 SYLFFQLKSQHNLYGPLDEEVDDGDADASEMFAWEAIGWLAILTVWVSVLSGYLVDAIQG 300
           SYLFFQLKSQ +LYGPLDE  +D   D SE++AWEAIGWLAILTVWVS+LSGYLVDAIQG
Sbjct: 241 SYLFFQLKSQQDLYGPLDEVGEDEVEDDSEIYAWEAIGWLAILTVWVSILSGYLVDAIQG 300

Query: 301 ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC 360
           ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC
Sbjct: 301 ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC 360

Query: 361 VVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420
           VVVGWCMG+PMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKG+MLILCYLIVAASFFV
Sbjct: 361 VVVGWCMGEPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGVMLILCYLIVAASFFV 420

Query: 421 HVDPSNGE 428
           H+DP+N  
Sbjct: 421 HIDPTNDN 427

BLAST of MC08g_new0124 vs. ExPASy TrEMBL
Match: A0A6J1GWA3 (Vacuolar cation/proton exchanger OS=Cucurbita moschata OX=3662 GN=LOC111457727 PE=3 SV=1)

HSP 1 Score: 754 bits (1948), Expect = 1.67e-273
Identity = 388/428 (90.65%), Postives = 402/428 (93.93%), Query Frame = 0

Query: 1   MGSIEEKTNYLSDEEIPFRSSPVAKNVLHSFDFETAHVRSSPDASTWWNINNIRIRPLKS 60
           MGS EEKTNYLSDE  P +SSPVAKNV HSFD ET  V SSPD + WW  NNIRIRPL+S
Sbjct: 1   MGSTEEKTNYLSDEGTPLKSSPVAKNVSHSFDCETGPVHSSPDVTRWWKTNNIRIRPLRS 60

Query: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG 120
           AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGI PLAERLGYATEQLAFYTG
Sbjct: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGIAPLAERLGYATEQLAFYTG 120

Query: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH 180
           PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGI+HH
Sbjct: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH 180

Query: 181 KKVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHFGESALSLSRFSSCIMLVAYA 240
           K VQ FNKG+AVVNSGLLLMAVMGTTFPAVLHFTHTELH GESALSLSRFSSCIML+AYA
Sbjct: 181 K-VQVFNKGSAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA 240

Query: 241 SYLFFQLKSQHNLYGPLDEEVDDGDADASEMFAWEAIGWLAILTVWVSVLSGYLVDAIQG 300
           SYLFFQLKSQ +LYGPLDE  +D   D SE++AWEAIGWLAILTVWVS+LSGYLVDAIQG
Sbjct: 241 SYLFFQLKSQQDLYGPLDEVGEDEVEDDSEIYAWEAIGWLAILTVWVSILSGYLVDAIQG 300

Query: 301 ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC 360
           ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC
Sbjct: 301 ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC 360

Query: 361 VVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420
           VVVGWCMG+PMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV
Sbjct: 361 VVVGWCMGEPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420

Query: 421 HVDPSNGE 428
           H+DP+N  
Sbjct: 421 HIDPTNDN 427

BLAST of MC08g_new0124 vs. TAIR 10
Match: AT1G55730.1 (cation exchanger 5 )

HSP 1 Score: 542.7 bits (1397), Expect = 2.6e-154
Identity = 282/372 (75.81%), Postives = 318/372 (85.48%), Query Frame = 0

Query: 58  LKSAYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAF 117
           L+S  IV++  K+N+LLPFGPLAILLHYLT   GW+F  +L+GITPLAERLGYATEQLA 
Sbjct: 65  LQSFKIVILSNKLNLLLPFGPLAILLHYLTDNKGWIFLLSLVGITPLAERLGYATEQLAC 124

Query: 118 YTGPTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGI 177
           YTG TVGGLLNATFGN TE+IISI+ALKSGMIRVVQ +LLGSILSNMLLVLGCAFF GG+
Sbjct: 125 YTGSTVGGLLNATFGNVTELIISIFALKSGMIRVVQLTLLGSILSNMLLVLGCAFFCGGL 184

Query: 178 IHHKKVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHFGESALSLSRFSSCIMLV 237
           +  +K Q F+KG AVVNSGLLLMAVMG  FPAVLH+TH+E+H G S L+LSRFSSCIMLV
Sbjct: 185 VFSQKEQVFDKGNAVVNSGLLLMAVMGLLFPAVLHYTHSEVHAGSSELALSRFSSCIMLV 244

Query: 238 AYASYLFFQLKSQHNLYGPLDEEVDDG-----DADASEMFAWEAIGWLAILTVWVSVLSG 297
           AYA+YLFFQLKSQ + Y PL EE +       D +  E+  WEAI WL+ILT WVS+LSG
Sbjct: 245 AYAAYLFFQLKSQPSSYTPLTEETNQNEETSDDDEDPEISKWEAIIWLSILTAWVSLLSG 304

Query: 298 YLVDAIQGASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQI 357
           YLVDAI+GAS S  +P++FISVILLPIVGNAAEHA AIMFAMKDKLD++LGVAIGSS QI
Sbjct: 305 YLVDAIEGASVSWKIPISFISVILLPIVGNAAEHAGAIMFAMKDKLDLSLGVAIGSSIQI 364

Query: 358 SMFVIPFCVVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYL 417
           SMF +PFCVV+GW MG  MDLNF+LFETATLFITV+VVAF LQEGTSNYFKGLMLILCYL
Sbjct: 365 SMFAVPFCVVIGWMMGAQMDLNFQLFETATLFITVIVVAFFLQEGTSNYFKGLMLILCYL 424

Query: 418 IVAASFFVHVDP 425
           IVAASFFVH DP
Sbjct: 425 IVAASFFVHEDP 436

BLAST of MC08g_new0124 vs. TAIR 10
Match: AT1G55730.2 (cation exchanger 5 )

HSP 1 Score: 542.7 bits (1397), Expect = 2.6e-154
Identity = 282/372 (75.81%), Postives = 318/372 (85.48%), Query Frame = 0

Query: 58  LKSAYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAF 117
           L+S  IV++  K+N+LLPFGPLAILLHYLT   GW+F  +L+GITPLAERLGYATEQLA 
Sbjct: 65  LQSFKIVILSNKLNLLLPFGPLAILLHYLTDNKGWIFLLSLVGITPLAERLGYATEQLAC 124

Query: 118 YTGPTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGI 177
           YTG TVGGLLNATFGN TE+IISI+ALKSGMIRVVQ +LLGSILSNMLLVLGCAFF GG+
Sbjct: 125 YTGSTVGGLLNATFGNVTELIISIFALKSGMIRVVQLTLLGSILSNMLLVLGCAFFCGGL 184

Query: 178 IHHKKVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHFGESALSLSRFSSCIMLV 237
           +  +K Q F+KG AVVNSGLLLMAVMG  FPAVLH+TH+E+H G S L+LSRFSSCIMLV
Sbjct: 185 VFSQKEQVFDKGNAVVNSGLLLMAVMGLLFPAVLHYTHSEVHAGSSELALSRFSSCIMLV 244

Query: 238 AYASYLFFQLKSQHNLYGPLDEEVDDG-----DADASEMFAWEAIGWLAILTVWVSVLSG 297
           AYA+YLFFQLKSQ + Y PL EE +       D +  E+  WEAI WL+ILT WVS+LSG
Sbjct: 245 AYAAYLFFQLKSQPSSYTPLTEETNQNEETSDDDEDPEISKWEAIIWLSILTAWVSLLSG 304

Query: 298 YLVDAIQGASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQI 357
           YLVDAI+GAS S  +P++FISVILLPIVGNAAEHA AIMFAMKDKLD++LGVAIGSS QI
Sbjct: 305 YLVDAIEGASVSWKIPISFISVILLPIVGNAAEHAGAIMFAMKDKLDLSLGVAIGSSIQI 364

Query: 358 SMFVIPFCVVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYL 417
           SMF +PFCVV+GW MG  MDLNF+LFETATLFITV+VVAF LQEGTSNYFKGLMLILCYL
Sbjct: 365 SMFAVPFCVVIGWMMGAQMDLNFQLFETATLFITVIVVAFFLQEGTSNYFKGLMLILCYL 424

Query: 418 IVAASFFVHVDP 425
           IVAASFFVH DP
Sbjct: 425 IVAASFFVHEDP 436

BLAST of MC08g_new0124 vs. TAIR 10
Match: AT3G13320.1 (cation exchanger 2 )

HSP 1 Score: 542.7 bits (1397), Expect = 2.6e-154
Identity = 279/372 (75.00%), Postives = 318/372 (85.48%), Query Frame = 0

Query: 58  LKSAYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAF 117
           L S  IV+   K+N+LLPFGPLAIL+HY+    GWVF  TL+GITPLAERLGYATEQLA 
Sbjct: 65  LNSIKIVIFCNKLNLLLPFGPLAILVHYMIDSKGWVFLLTLVGITPLAERLGYATEQLAC 124

Query: 118 YTGPTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGI 177
           YTGPTVGGLLNATFGN TE+IISI+ALK+GMIRVVQ +LLGSILSNMLLVLGCAFF GG+
Sbjct: 125 YTGPTVGGLLNATFGNVTELIISIFALKNGMIRVVQLTLLGSILSNMLLVLGCAFFCGGL 184

Query: 178 IHHKKVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHFGESALSLSRFSSCIMLV 237
           + ++K Q F+KG A VNSGLLLMAVMG  FPAVLH+TH+E+H G S L+LSRFSSCIML+
Sbjct: 185 VFYQKDQVFDKGIATVNSGLLLMAVMGILFPAVLHYTHSEVHAGSSELALSRFSSCIMLI 244

Query: 238 AYASYLFFQLKSQHNLYGPLDEEVDDGDADAS-----EMFAWEAIGWLAILTVWVSVLSG 297
           AYA+YLFFQLKSQ N Y PLDEE +  +  ++     E+  WEAI WL+ILT WVS+LSG
Sbjct: 245 AYAAYLFFQLKSQSNSYSPLDEESNQNEETSAEDEDPEISKWEAIIWLSILTAWVSLLSG 304

Query: 298 YLVDAIQGASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQI 357
           YLVDAI+GAS S N+P+AFIS ILLPIVGNAAEHA AIMFAMKDKLD++LGVAIGSS QI
Sbjct: 305 YLVDAIEGASVSWNIPIAFISTILLPIVGNAAEHAGAIMFAMKDKLDLSLGVAIGSSIQI 364

Query: 358 SMFVIPFCVVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYL 417
           SMF +PFCVV+GW MG+ MDLNF+LFETA LFITV+VVAF LQEG+SNYFKGLMLILCYL
Sbjct: 365 SMFAVPFCVVIGWMMGQQMDLNFQLFETAMLFITVIVVAFFLQEGSSNYFKGLMLILCYL 424

Query: 418 IVAASFFVHVDP 425
           IVAASFFVH DP
Sbjct: 425 IVAASFFVHEDP 436

BLAST of MC08g_new0124 vs. TAIR 10
Match: AT1G55720.1 (cation exchanger 6 )

HSP 1 Score: 499.6 bits (1285), Expect = 2.5e-141
Identity = 265/374 (70.86%), Postives = 305/374 (81.55%), Query Frame = 0

Query: 58  LKSAYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAF 117
           ++S  IV++  K+N+LLPFGPLAIL+HYLT   GW F  +L+GITPLAERLGYATEQL+ 
Sbjct: 76  VQSFKIVILSNKLNLLLPFGPLAILVHYLTDNKGWFFLLSLVGITPLAERLGYATEQLSC 135

Query: 118 YTGPTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGI 177
           YTG TVGGLLNATFGN  E+IISI ALK+GMIRVVQ +LLGSILSN+LLVLGCAFF GG+
Sbjct: 136 YTGATVGGLLNATFGNVIELIISIIALKNGMIRVVQLTLLGSILSNILLVLGCAFFCGGL 195

Query: 178 IHHKKVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHFGESALSLSRFSSCIMLV 237
           +   K Q F+K  AVV+SG+LLMAVMG  FP  LH+TH+E+H G S L+LSRF SCIMLV
Sbjct: 196 VFPGKDQVFDKRNAVVSSGMLLMAVMGLLFPTFLHYTHSEVHAGSSELALSRFISCIMLV 255

Query: 238 AYASYLFFQLKSQHNLYGP---LDEEVDDGDADASEMFAWEAIGWLAILTVWVSVLSGYL 297
           AYA+YLFFQLKSQ + Y      +EE  + D D  E+  WEAI WL+I T WVS+LSGYL
Sbjct: 256 AYAAYLFFQLKSQPSFYTEKTNQNEETSNDDED-PEISKWEAIIWLSIFTAWVSLLSGYL 315

Query: 298 VDAIQGASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISM 357
           VDAI+G S S  +P++FISVILLPIVGNAAEHA AIMFAMKDKLD++LGVAIGSS QISM
Sbjct: 316 VDAIEGTSVSWKIPISFISVILLPIVGNAAEHAGAIMFAMKDKLDLSLGVAIGSSIQISM 375

Query: 358 FVIPFCVVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQ-EGTSNYFKGLMLILCYLI 417
           F +PFCVV+GW MG  MDLN +LFETATL ITV+VVAF LQ EGTSNYFK LMLILCYLI
Sbjct: 376 FAVPFCVVIGWMMGAQMDLNLQLFETATLLITVIVVAFFLQLEGTSNYFKRLMLILCYLI 435

Query: 418 VAASFFVHVDPSNG 428
           VAASFFVH DP  G
Sbjct: 436 VAASFFVHEDPHQG 448

BLAST of MC08g_new0124 vs. TAIR 10
Match: AT5G01490.1 (cation exchanger 4 )

HSP 1 Score: 348.2 bits (892), Expect = 9.4e-96
Identity = 190/380 (50.00%), Postives = 261/380 (68.68%), Query Frame = 0

Query: 56  RPLKSAYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQL 115
           R L +   VL+  K+ +L P  PLA++ H       WVF  +LLG+TPLAER+ + TEQ+
Sbjct: 62  RILTNLQEVLLGTKLFILFPAVPLAVVAHRYDCPRAWVFALSLLGLTPLAERISFLTEQI 121

Query: 116 AFYTGPTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTG 175
           AF+TGPTVGGL+NAT GNATEMII+I A+    +R+V+ SLLGSILSN+L VLG + F G
Sbjct: 122 AFHTGPTVGGLMNATCGNATEMIIAILAVGQRKMRIVKLSLLGSILSNLLFVLGTSLFLG 181

Query: 176 GIIHHKKVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFT-HTELHFGESALSLSRFSSCI 235
           GI + +K Q+F+     +NS LL +A++  T P ++ FT   E + G   + LSR SS +
Sbjct: 182 GISNLRKHQSFDPRQGDMNSMLLYLALLCQTLPMIMRFTMEAEEYDGSDVVVLSRASSFV 241

Query: 236 MLVAYASYLFFQLKSQHNLYGP---------LDEEVDDGDADASEMFAWEAIGWLAILTV 295
           ML+AY ++L F L S H    P          D++V D + + + +  W AI WL I+T+
Sbjct: 242 MLIAYLAFLIFHLFSSHLSPPPPPLPQREDVHDDDVSDKEEEGAVIGMWSAIFWLIIMTL 301

Query: 296 WVSVLSGYLVDAIQGASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVA 355
            V++LS YLV  IQ A++S  L V FI +ILLPIVGNAAEHA A++FA ++KLDITLG+A
Sbjct: 302 LVALLSDYLVSTIQDAADSWGLSVGFIGIILLPIVGNAAEHAGAVIFAFRNKLDITLGIA 361

Query: 356 IGSSTQISMFVIPFCVVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGL 415
           +GS+TQI++FV+P  V+V W MG  MDLNF L ETA   +++LV + +LQ+GTSNY KGL
Sbjct: 362 LGSATQIALFVVPVTVLVAWTMGIEMDLNFNLLETACFALSILVTSLVLQDGTSNYMKGL 421

Query: 416 MLILCYLIVAASFFVHVDPS 426
           +L+LCY+++AA FFV   PS
Sbjct: 422 VLLLCYVVIAACFFVSNSPS 441

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5KQN05.8e-16774.40Vacuolar cation/proton exchanger 2 OS=Oryza sativa subsp. japonica OX=39947 GN=C... [more]
Q6K1C41.1e-16075.91Vacuolar cation/proton exchanger 3 OS=Oryza sativa subsp. japonica OX=39947 GN=C... [more]
Q392543.6e-15375.00Vacuolar cation/proton exchanger 2 OS=Arabidopsis thaliana OX=3702 GN=CAX2 PE=1 ... [more]
Q8L7833.6e-15375.81Vacuolar cation/proton exchanger 5 OS=Arabidopsis thaliana OX=3702 GN=CAX5 PE=2 ... [more]
Q9LFZ83.5e-14070.86Putative vacuolar cation/proton exchanger 6 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Match NameE-valueIdentityDescription
XP_022152617.12.07e-30499.53vacuolar cation/proton exchanger 3-like [Momordica charantia][more]
XP_008463979.11.29e-28494.39PREDICTED: vacuolar cation/proton exchanger 3-like [Cucumis melo] >XP_008463980.... [more]
NP_001267651.13.41e-28293.69vacuolar cation/proton exchanger 2-like [Cucumis sativus] >XP_011650230.1 vacuol... [more]
XP_038902338.13.79e-28093.02vacuolar cation/proton exchanger 3-like [Benincasa hispida][more]
XP_023526825.12.96e-27490.89vacuolar cation/proton exchanger 2-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1DGR61.00e-30499.53Vacuolar cation/proton exchanger OS=Momordica charantia OX=3673 GN=LOC111020296 ... [more]
A0A1S3CKH86.26e-28594.39Vacuolar cation/proton exchanger OS=Cucumis melo OX=3656 GN=LOC103501973 PE=3 SV... [more]
C8CBQ51.65e-28293.69Vacuolar cation/proton exchanger OS=Cucumis sativus OX=3659 GN=CAX2 PE=2 SV=1[more]
A0A6J1IRU58.29e-27490.65Vacuolar cation/proton exchanger OS=Cucurbita maxima OX=3661 GN=LOC111479941 PE=... [more]
A0A6J1GWA31.67e-27390.65Vacuolar cation/proton exchanger OS=Cucurbita moschata OX=3662 GN=LOC111457727 P... [more]
Match NameE-valueIdentityDescription
AT1G55730.12.6e-15475.81cation exchanger 5 [more]
AT1G55730.22.6e-15475.81cation exchanger 5 [more]
AT3G13320.12.6e-15475.00cation exchanger 2 [more]
AT1G55720.12.5e-14170.86cation exchanger 6 [more]
AT5G01490.19.4e-9650.00cation exchanger 4 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004798Calcium/proton exchanger CAX-likeTIGRFAMTIGR00378TIGR00378coord: 73..419
e-value: 1.7E-101
score: 337.8
NoneNo IPR availableTIGRFAMTIGR00846TIGR00846coord: 63..419
e-value: 2.7E-115
score: 383.1
NoneNo IPR availablePANTHERPTHR31503:SF54VACUOLAR CATION/PROTON EXCHANGERcoord: 1..427
IPR044880NCX, central ion-binding domain superfamilyGENE3D1.20.1420.30coord: 121..261
e-value: 6.7E-16
score: 60.3
coord: 267..420
e-value: 1.2E-17
score: 66.0
IPR004837Sodium/calcium exchanger membrane regionPFAMPF01699Na_Ca_excoord: 92..247
e-value: 1.0E-20
score: 74.2
coord: 277..416
e-value: 2.9E-19
score: 69.5
IPR004713Calcium/proton exchangerPANTHERPTHR31503VACUOLAR CALCIUM ION TRANSPORTERcoord: 1..427

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g_new0124.1MC08g_new0124.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070588 calcium ion transmembrane transport
biological_process GO:0006874 cellular calcium ion homeostasis
biological_process GO:0006816 calcium ion transport
biological_process GO:0006812 cation transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009705 plant-type vacuole membrane
molecular_function GO:0015369 calcium:proton antiporter activity
molecular_function GO:0008324 cation transmembrane transporter activity