MC08g2255 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g2255 vs. ExPASy Swiss-Prot
Match: Q43190 (Probable linoleate 9S-lipoxygenase 4 OS=Solanum tuberosum OX=4113 GN=LOX1.4 PE=2 SV=1)

HSP 1 Score: 1158.3 bits (2995), Expect = 0.0e+00
Identity = 560/844 (66.35%), Postives = 676/844 (80.09%), Query Frame = 0

Query: 39  KKIKGKVILMRSNVLDFTEFHSTLLDGFTELLGGGVSLQLISATQASLDS--RGKVGKRA 98
           KK+KG V++M+ N LDFT+   +L D   E LG  VS QLIS+ Q+   +  +GK    A
Sbjct: 17  KKVKGTVVMMKKNALDFTDLAGSLTDKIFEALGQKVSFQLISSVQSDPANGLQGKHSNPA 76

Query: 99  FLERWLTSIPPLFAGESVFQVNFDLEDNFGLPGAFFIKNGHTSEFFLKSLTLEDVPGFGR 158
           +LE +L ++ PL AGE+ F V FD  + FG+PGAF IKN H +EFFLKSLTLEDVP  G+
Sbjct: 77  YLENFLFTLTPLAAGETAFGVTFDWNEEFGVPGAFIIKNTHINEFFLKSLTLEDVPNHGK 136

Query: 159 IHFDCNSWVYPSGRYKKDRIFFANKTFLPSETPNPLRKYREEELLNLRGDGTGERKEWDR 218
           +HF CNSWVYPS RYK DRIFFAN+ +LPSETP  LRKYRE ELL LRGDGTG+R+ WDR
Sbjct: 137 VHFVCNSWVYPSFRYKSDRIFFANQPYLPSETPELLRKYRENELLTLRGDGTGKREAWDR 196

Query: 219 IYDYDVYNDLSDP-----NVRPILGGS-QHPYPRRGRTGRPRARRDSNYETRLSAVLGLT 278
           IYDYDVYNDL +P     NVR  LGGS  +PYPRRGRTGRP  R D   E+R+  +L L 
Sbjct: 197 IYDYDVYNDLGNPDQGKENVRTTLGGSADYPYPRRGRTGRPPTRTDPKSESRIPLILSLD 256

Query: 279 IYVPRDENFGHLKMSDFLGFALKSLSSTVQPELVNLINITPGEFDKFQDVHDLYEGGFPV 338
           IYVPRDE FGHLKMSDFL +ALKS+   + PEL  L + TP EFD F+DV  LYEGG  +
Sbjct: 257 IYVPRDERFGHLKMSDFLTYALKSIVQFILPELHALFDGTPNEFDSFEDVLRLYEGGIRL 316

Query: 339 PLN-VFRNLTEAFTPPMFQELLRNDGERFLKFSPPQVVRDNKSGWRTDEEFAREMLAGVN 398
           P   +F+ LT+A    M +ELLR DGE  L+F  P V++D+K+ WRTDEEFAREMLAGVN
Sbjct: 317 PQGPLFKALTDAIPLEMIRELLRTDGEGILRFPTPLVIKDSKTAWRTDEEFAREMLAGVN 376

Query: 399 PVIICRLKEFPPLSKLDPQIYGDQNSKITEEDIKFGLEGLSADEAIKQNKLYILDHHDAL 458
           P+II RL+EFPP SKLDP+ YG+QNS IT E I+  L+GL+ DEA+  NKL+IL+HHD L
Sbjct: 377 PIIISRLQEFPPKSKLDPEAYGNQNSTITAEHIEDKLDGLTVDEAMNNNKLFILNHHDVL 436

Query: 459 MPYLRRINSTSTRTYATRTLLFLKADGTLKPLVIELSLPHPQGDQFGANSKLYFPAESGV 518
           +PYLRRIN+T+T+TYA+RTLLFL+ +G+LKPL IELSLPHP GDQFG  SK+Y P++ GV
Sbjct: 437 IPYLRRINTTTTKTYASRTLLFLQDNGSLKPLAIELSLPHPDGDQFGVTSKVYTPSDQGV 496

Query: 519 ENSIWQLAKAYVAVNDAGYHQLNSHWLHTHAVQEPFVIATHRQLSVVHPIHKLLVPHFKD 578
           E+SIWQLAKAYVAVND+G HQL SHWL+THAV EPFVIAT+RQLSV+HPIHKLL PHF+D
Sbjct: 497 ESSIWQLAKAYVAVNDSGVHQLISHWLNTHAVIEPFVIATNRQLSVLHPIHKLLYPHFRD 556

Query: 579 TMFINAFARQVLVNADGLLESTHFQSKYAMELSSDVYKEWNFLEQGLPADLIKRGVAVED 638
           TM INA ARQ+L+NA G+LEST F SK+AME+S+ VYK+W F +Q LPADL+KRGVAVED
Sbjct: 557 TMNINAMARQILINAGGVLESTVFPSKFAMEMSAVVYKDWVFPDQALPADLVKRGVAVED 616

Query: 639 SNSPHGLRLLIEDYPFAVDGLEIWSTIKTWVTDYISVYYKDDKAVQNDVELQSWWKEVRE 698
           S+SPHG+RLLIEDYP+AVDGLEIWS IK+WVTDY S YY  D+ +  D ELQ+WWKE+RE
Sbjct: 617 SSSPHGVRLLIEDYPYAVDGLEIWSAIKSWVTDYCSFYYGSDEEILKDNELQAWWKELRE 676

Query: 699 KGHADKKNEPWWPKMQTLKELIESCTTIIWISSALHAAVNFGQYPYGGFIPNRPTISRRF 758
            GH DKKNEPWWP+M+T +ELI+SCTTIIWI+SALHAAVNFGQYPY G++PNRPT+SRRF
Sbjct: 677 VGHGDKKNEPWWPEMETPQELIDSCTTIIWIASALHAAVNFGQYPYAGYLPNRPTVSRRF 736

Query: 759 MPEVGTPEYKELESNPEKAFLRTINSQLQTLLGVSLIEILSRHASDEVYLGQRASIEWTS 818
           MPE GTPEY+EL+ NP+KAFL+TI +QLQTLLGVSLIEILSRH +DE+YLGQR S EWT 
Sbjct: 737 MPEPGTPEYEELKKNPDKAFLKTITAQLQTLLGVSLIEILSRHTTDEIYLGQRESPEWTK 796

Query: 819 DAAAIEVFEKFGKEVFEVENRIIERNKNINFKNRTGPVNVPYTLLLPSSTEGLTGRGIPN 874
           D   +  F+KFGK++ ++E +II+RN +    NR+GPVN PYTLL P+S  GLTG+GIPN
Sbjct: 797 DKEPLAAFDKFGKKLTDIEKQIIQRNGDNILINRSGPVNAPYTLLFPTSEGGLTGKGIPN 856

BLAST of MC08g2255 vs. ExPASy Swiss-Prot
Match: Q41238 (Linoleate 9S-lipoxygenase 6 (Fragment) OS=Solanum tuberosum OX=4113 GN=LOX1.6 PE=1 SV=1)

HSP 1 Score: 1157.1 bits (2992), Expect = 0.0e+00
Identity = 560/844 (66.35%), Postives = 675/844 (79.98%), Query Frame = 0

Query: 39  KKIKGKVILMRSNVLDFTEFHSTLLDGFTELLGGGVSLQLISATQASLDS--RGKVGKRA 98
           KK+KG V++M+ N LDFT+   +L D   E LG  VS QLIS+ Q+   +  +GK    A
Sbjct: 14  KKVKGTVVMMKKNALDFTDLAGSLTDKIFEALGQKVSFQLISSVQSDPANGLQGKHSNPA 73

Query: 99  FLERWLTSIPPLFAGESVFQVNFDLEDNFGLPGAFFIKNGHTSEFFLKSLTLEDVPGFGR 158
           +LE +L ++ PL AGE+ F V FD  + FG+PGAF IKN H +EFFLKSLTLEDVP  G+
Sbjct: 74  YLENFLFTLTPLAAGETAFGVTFDWNEEFGVPGAFIIKNTHINEFFLKSLTLEDVPNHGK 133

Query: 159 IHFDCNSWVYPSGRYKKDRIFFANKTFLPSETPNPLRKYREEELLNLRGDGTGERKEWDR 218
           +HF CNSWVYPS RYK DRIFFAN+ +LPSETP  LRKYRE ELL LRGDGTG+R+ WDR
Sbjct: 134 VHFVCNSWVYPSFRYKSDRIFFANQPYLPSETPELLRKYRENELLTLRGDGTGKREAWDR 193

Query: 219 IYDYDVYNDLSDP-----NVRPILGGS-QHPYPRRGRTGRPRARRDSNYETRLSAVLGLT 278
           IYDYDVYNDL +P     NVR  LGGS  +PYPRRGRTGRP  R D   E+R+  +L L 
Sbjct: 194 IYDYDVYNDLGNPDQGEQNVRTTLGGSADYPYPRRGRTGRPPTRTDPKSESRIPLILSLD 253

Query: 279 IYVPRDENFGHLKMSDFLGFALKSLSSTVQPELVNLINITPGEFDKFQDVHDLYEGGFPV 338
           IYVPRDE FGHLKMSDFL +ALKS+   + PEL  L + TP EFD F+DV  LYEGG  +
Sbjct: 254 IYVPRDERFGHLKMSDFLTYALKSIVQFILPELHALFDGTPNEFDSFEDVLRLYEGGIKL 313

Query: 339 PLN-VFRNLTEAFTPPMFQELLRNDGERFLKFSPPQVVRDNKSGWRTDEEFAREMLAGVN 398
           P   +F+ LT A    M +ELLR DGE  L+F  P V++D+K+ WRTDEEFAREMLAGVN
Sbjct: 314 PQGPLFKALTAAIPLEMMKELLRTDGEGILRFPTPLVIKDSKTAWRTDEEFAREMLAGVN 373

Query: 399 PVIICRLKEFPPLSKLDPQIYGDQNSKITEEDIKFGLEGLSADEAIKQNKLYILDHHDAL 458
           P+II RL+EFPP SKLDP+ YG+QNS IT E I+  L+GL+ DEA+  NKL+IL+HHD L
Sbjct: 374 PIIISRLQEFPPKSKLDPEAYGNQNSTITAEHIEDKLDGLTVDEAMNNNKLFILNHHDVL 433

Query: 459 MPYLRRINSTSTRTYATRTLLFLKADGTLKPLVIELSLPHPQGDQFGANSKLYFPAESGV 518
           +PYLRRIN+T+T+TYA+RTLLFL+ +G+LKPL IELSLPHP GDQFG  SK+Y P++ GV
Sbjct: 434 IPYLRRINTTTTKTYASRTLLFLQDNGSLKPLAIELSLPHPDGDQFGVISKVYTPSDQGV 493

Query: 519 ENSIWQLAKAYVAVNDAGYHQLNSHWLHTHAVQEPFVIATHRQLSVVHPIHKLLVPHFKD 578
           E+SIWQLAKAYVAVND+G HQL SHWL+THAV EPFVIAT+RQLSV+HPIHKLL PHF+D
Sbjct: 494 ESSIWQLAKAYVAVNDSGVHQLISHWLNTHAVIEPFVIATNRQLSVLHPIHKLLYPHFRD 553

Query: 579 TMFINAFARQVLVNADGLLESTHFQSKYAMELSSDVYKEWNFLEQGLPADLIKRGVAVED 638
           TM INA ARQ+L+NA G+LEST F SK+AME+S+ VYK+W F +Q LPADL+KRGVAVED
Sbjct: 554 TMNINAMARQILINAGGVLESTVFPSKFAMEMSAVVYKDWVFPDQALPADLVKRGVAVED 613

Query: 639 SNSPHGLRLLIEDYPFAVDGLEIWSTIKTWVTDYISVYYKDDKAVQNDVELQSWWKEVRE 698
           S+SPHG+RLLIEDYP+AVDGLEIWS IK+WVTDY S YY  D+ +  D ELQ+WWKE+RE
Sbjct: 614 SSSPHGVRLLIEDYPYAVDGLEIWSAIKSWVTDYCSFYYGSDEEILKDNELQAWWKELRE 673

Query: 699 KGHADKKNEPWWPKMQTLKELIESCTTIIWISSALHAAVNFGQYPYGGFIPNRPTISRRF 758
            GH DKKNEPWWP+M+T +ELI+SCTTIIWI+SALHAAVNFGQYPY G++PNRPT+SRRF
Sbjct: 674 VGHGDKKNEPWWPEMETPQELIDSCTTIIWIASALHAAVNFGQYPYAGYLPNRPTVSRRF 733

Query: 759 MPEVGTPEYKELESNPEKAFLRTINSQLQTLLGVSLIEILSRHASDEVYLGQRASIEWTS 818
           MPE GTPEY+EL+ NP+KAFL+TI +QLQTLLGVSLIEILSRH +DE+YLGQR S EWT 
Sbjct: 734 MPEPGTPEYEELKKNPDKAFLKTITAQLQTLLGVSLIEILSRHTTDEIYLGQRESPEWTK 793

Query: 819 DAAAIEVFEKFGKEVFEVENRIIERNKNINFKNRTGPVNVPYTLLLPSSTEGLTGRGIPN 874
           D   +  F+KFGK++ ++E +II+RN +    NR+GPVN PYTLL P+S  GLTG+GIPN
Sbjct: 794 DKEPLAAFDKFGKKLTDIEKQIIQRNGDNILTNRSGPVNAPYTLLFPTSEGGLTGKGIPN 853

BLAST of MC08g2255 vs. ExPASy Swiss-Prot
Match: P38415 (Linoleate 9S-lipoxygenase A OS=Solanum lycopersicum OX=4081 GN=LOX1.1 PE=2 SV=1)

HSP 1 Score: 1154.0 bits (2984), Expect = 0.0e+00
Identity = 558/844 (66.11%), Postives = 674/844 (79.86%), Query Frame = 0

Query: 39  KKIKGKVILMRSNVLDFTEFHSTLLDGFTELLGGGVSLQLISATQASLDS--RGKVGKRA 98
           KK+KG V++M+ N LDFT+   +L D   E LG  VS QLIS+ Q+   +  +GK    A
Sbjct: 17  KKVKGTVVMMKKNALDFTDLAGSLTDKIFEALGQKVSFQLISSVQSDPANGLQGKHSNPA 76

Query: 99  FLERWLTSIPPLFAGESVFQVNFDLEDNFGLPGAFFIKNGHTSEFFLKSLTLEDVPGFGR 158
           +LE +L ++ PL AGE+ F V FD  + FG+PGAF IKN H +EFFLKSLTLEDVP  G+
Sbjct: 77  YLENFLLTLTPLAAGETAFGVTFDWNEEFGVPGAFVIKNMHINEFFLKSLTLEDVPNHGK 136

Query: 159 IHFDCNSWVYPSGRYKKDRIFFANKTFLPSETPNPLRKYREEELLNLRGDGTGERKEWDR 218
           +HF CNSWVYPS RYK DRIFFAN+ +LPSETP  LRKYRE EL+ LRGDGTG+R+ WDR
Sbjct: 137 VHFVCNSWVYPSFRYKSDRIFFANQPYLPSETPELLRKYRENELVTLRGDGTGKREAWDR 196

Query: 219 IYDYDVYNDLSDP-----NVRPILGGS-QHPYPRRGRTGRPRARRDSNYETRLSAVLGLT 278
           IYDYDVYNDL +P     NVR  LGGS  +PYPRRGRTGRP  R D   E+R+  +L L 
Sbjct: 197 IYDYDVYNDLGNPDQGKENVRTTLGGSADYPYPRRGRTGRPPTRTDPKSESRIPLILSLD 256

Query: 279 IYVPRDENFGHLKMSDFLGFALKSLSSTVQPELVNLINITPGEFDKFQDVHDLYEGGFPV 338
           IYVPRDE FGHLKMSDFL +ALKS+   + PEL  L + TP EFD F+DV  LYEGG  +
Sbjct: 257 IYVPRDERFGHLKMSDFLTYALKSIVQFILPELHALFDGTPNEFDSFEDVLRLYEGGIKL 316

Query: 339 PLN-VFRNLTEAFTPPMFQELLRNDGERFLKFSPPQVVRDNKSGWRTDEEFAREMLAGVN 398
           P   +F+ LT+A    M +ELLR DGE  L+F  P V++D+K+ WRTDEEFAREMLAGVN
Sbjct: 317 PQGPLFKALTDAIPLEMIRELLRTDGEGILRFPTPLVIKDSKTAWRTDEEFAREMLAGVN 376

Query: 399 PVIICRLKEFPPLSKLDPQIYGDQNSKITEEDIKFGLEGLSADEAIKQNKLYILDHHDAL 458
           PVII RL+EFPP SKLDP++YG+QNS IT E I+  L+GL+ DEAI  NKL+IL+HHD L
Sbjct: 377 PVIISRLEEFPPKSKLDPELYGNQNSTITAEHIEGKLDGLTIDEAINSNKLFILNHHDVL 436

Query: 459 MPYLRRINSTSTRTYATRTLLFLKADGTLKPLVIELSLPHPQGDQFGANSKLYFPAESGV 518
           +PYLRRIN+T+T+TYA+RTLLFL+ +G+LKPL IELSLPHP GDQFG  SK+Y P++ GV
Sbjct: 437 IPYLRRINTTTTKTYASRTLLFLQDNGSLKPLAIELSLPHPDGDQFGVTSKVYTPSDQGV 496

Query: 519 ENSIWQLAKAYVAVNDAGYHQLNSHWLHTHAVQEPFVIATHRQLSVVHPIHKLLVPHFKD 578
           E SIWQLAKAYVAVND+G HQL SHWL+THAV EPFVIAT+RQLSV+HPIHKLL PHF+D
Sbjct: 497 EGSIWQLAKAYVAVNDSGVHQLISHWLNTHAVIEPFVIATNRQLSVLHPIHKLLYPHFRD 556

Query: 579 TMFINAFARQVLVNADGLLESTHFQSKYAMELSSDVYKEWNFLEQGLPADLIKRGVAVED 638
           TM INA ARQ+L+NA G+LEST F SK+AME+S+ VYK+W F +Q LPADL+KRGVAVED
Sbjct: 557 TMNINALARQILINAGGVLESTVFPSKFAMEMSAVVYKDWVFPDQALPADLVKRGVAVED 616

Query: 639 SNSPHGLRLLIEDYPFAVDGLEIWSTIKTWVTDYISVYYKDDKAVQNDVELQSWWKEVRE 698
           S+SPHG+RLLI+DYP+AVDGLEIWS IK+WVTDY S YY  ++ +  D ELQ+WWKEVRE
Sbjct: 617 SSSPHGVRLLIDDYPYAVDGLEIWSAIKSWVTDYCSFYYGSNEEILKDNELQAWWKEVRE 676

Query: 699 KGHADKKNEPWWPKMQTLKELIESCTTIIWISSALHAAVNFGQYPYGGFIPNRPTISRRF 758
            GH DKKNEPWW +M+T +ELI+SCTTIIWI+SALHAAVNFGQYPY G++PNRPT+SR+F
Sbjct: 677 VGHGDKKNEPWWAEMETPQELIDSCTTIIWIASALHAAVNFGQYPYAGYLPNRPTVSRKF 736

Query: 759 MPEVGTPEYKELESNPEKAFLRTINSQLQTLLGVSLIEILSRHASDEVYLGQRASIEWTS 818
           MPE GTPEY+EL+ NP+KAFL+TI +QLQTLLGVSLIEILSRH +DE+YLGQR S EWT 
Sbjct: 737 MPEPGTPEYEELKKNPDKAFLKTITAQLQTLLGVSLIEILSRHTTDEIYLGQRESPEWTK 796

Query: 819 DAAAIEVFEKFGKEVFEVENRIIERNKNINFKNRTGPVNVPYTLLLPSSTEGLTGRGIPN 874
           D   +  FE+FG ++ ++E +I++RN N    NRTGPVN PYTLL P+S  GLTG+GIPN
Sbjct: 797 DKEPLAAFERFGNKLTDIEKQIMQRNGNNILTNRTGPVNAPYTLLFPTSEGGLTGKGIPN 856

BLAST of MC08g2255 vs. ExPASy Swiss-Prot
Match: O24379 (Linoleate 9S-lipoxygenase 2 OS=Solanum tuberosum OX=4113 GN=LOX1.2 PE=1 SV=1)

HSP 1 Score: 1146.7 bits (2965), Expect = 0.0e+00
Identity = 557/845 (65.92%), Postives = 672/845 (79.53%), Query Frame = 0

Query: 39  KKIKGKVILMRSNVLDFTEFHSTLLDGFTELLGGGVSLQLISATQASLDS--RGKVGKRA 98
           KK+KG V++M  N LDFT+   +L D   E LG  VS QLIS+ Q    +  +GK    A
Sbjct: 17  KKLKGTVVMMNKNALDFTDLAGSLTDKAFEFLGQTVSFQLISSVQGDPTNGLQGKHSNPA 76

Query: 99  FLERWLTSIPPLFAG-ESVFQVNFDLEDNFGLPGAFFIKNGHTSEFFLKSLTLEDVPGFG 158
           +LE  L ++ PL AG E+ F V FD  + FG+PGAF IKN H +EFFLKSLTLEDVP  G
Sbjct: 77  YLENSLFTLTPLTAGSETAFGVTFDWNEEFGVPGAFIIKNTHINEFFLKSLTLEDVPNHG 136

Query: 159 RIHFDCNSWVYPSGRYKKDRIFFANKTFLPSETPNPLRKYREEELLNLRGDGTGERKEWD 218
           ++HF CNSWVYPS RYK DRIFF N+ +LPS+TP  LRKYRE ELL LRGDGTG+R+ WD
Sbjct: 137 KVHFVCNSWVYPSFRYKSDRIFFVNQPYLPSKTPELLRKYRENELLTLRGDGTGKREAWD 196

Query: 219 RIYDYDVYNDLSDP-----NVRPILGGS-QHPYPRRGRTGRPRARRDSNYETRLSAVLGL 278
           RIYDYD+YNDL +P     NVR  LGGS ++PYPRRGRTGRP  R D   E+R+  +L L
Sbjct: 197 RIYDYDIYNDLGNPDEGKENVRTTLGGSAEYPYPRRGRTGRPPTRTDPKSESRIPLILSL 256

Query: 279 TIYVPRDENFGHLKMSDFLGFALKSLSSTVQPELVNLINITPGEFDKFQDVHDLYEGGFP 338
            IYVPRDE FGHLKMSDFL +ALKS+   + PEL  L + TP EFD F+DV  LYEGG  
Sbjct: 257 DIYVPRDERFGHLKMSDFLTYALKSIVQFILPELHALFDGTPNEFDSFEDVLRLYEGGIK 316

Query: 339 VPLN-VFRNLTEAFTPPMFQELLRNDGERFLKFSPPQVVRDNKSGWRTDEEFAREMLAGV 398
           +P   +F+ LT A    M +ELLR DGE  L+F  P V++D+K+ WRTDEEFAREMLAGV
Sbjct: 317 LPQGPLFKALTAAIPLEMIRELLRTDGEGILRFPTPLVIKDSKTAWRTDEEFAREMLAGV 376

Query: 399 NPVIICRLKEFPPLSKLDPQIYGDQNSKITEEDIKFGLEGLSADEAIKQNKLYILDHHDA 458
           NPVII RL+EFPP SKLDP+ YG+QNS IT E I+  L+GL+ DEA+  NKL+IL+HHD 
Sbjct: 377 NPVIISRLQEFPPKSKLDPEAYGNQNSTITAEHIEDKLDGLTVDEAMNNNKLFILNHHDV 436

Query: 459 LMPYLRRINSTSTRTYATRTLLFLKADGTLKPLVIELSLPHPQGDQFGANSKLYFPAESG 518
           L+PYLRRIN+T+T+TYA+RTLLFL+ +G+LKPL IELSLPHP GDQFG  SK+Y P++ G
Sbjct: 437 LIPYLRRINTTTTKTYASRTLLFLQDNGSLKPLAIELSLPHPDGDQFGVTSKVYTPSDQG 496

Query: 519 VENSIWQLAKAYVAVNDAGYHQLNSHWLHTHAVQEPFVIATHRQLSVVHPIHKLLVPHFK 578
           VE+SIWQLAKAYVAVND+G HQL SHWL+THAV EPFVIAT+RQLSV+HPIHKLL PHF+
Sbjct: 497 VESSIWQLAKAYVAVNDSGVHQLISHWLNTHAVIEPFVIATNRQLSVLHPIHKLLYPHFR 556

Query: 579 DTMFINAFARQVLVNADGLLESTHFQSKYAMELSSDVYKEWNFLEQGLPADLIKRGVAVE 638
           DTM INA ARQ+L+NA G+LEST FQSK+AME+S+ VYK+W F +Q LPADL+KRGVAVE
Sbjct: 557 DTMNINAMARQILINAGGVLESTVFQSKFAMEMSAVVYKDWVFPDQALPADLVKRGVAVE 616

Query: 639 DSNSPHGLRLLIEDYPFAVDGLEIWSTIKTWVTDYISVYYKDDKAVQNDVELQSWWKEVR 698
           DS+SPHG+RLLIEDYP+AVDGLEIWS IK+WV+DY S YY  D+ +  D ELQ+WWKE+R
Sbjct: 617 DSSSPHGVRLLIEDYPYAVDGLEIWSAIKSWVSDYCSFYYGSDEEILKDNELQAWWKELR 676

Query: 699 EKGHADKKNEPWWPKMQTLKELIESCTTIIWISSALHAAVNFGQYPYGGFIPNRPTISRR 758
           E GH DKKNEPWWP+M+  +ELI+SCTTIIWI+SALHAAVNFGQYPY G++PNRPT+SRR
Sbjct: 677 EVGHGDKKNEPWWPEMERPQELIDSCTTIIWIASALHAAVNFGQYPYAGYLPNRPTVSRR 736

Query: 759 FMPEVGTPEYKELESNPEKAFLRTINSQLQTLLGVSLIEILSRHASDEVYLGQRASIEWT 818
           FMPE GTPEY+EL+ NP+KAFL+TI +QLQTLLGVSLIEILSRH +DE+YLGQR S EWT
Sbjct: 737 FMPEPGTPEYEELKKNPDKAFLKTITAQLQTLLGVSLIEILSRHTTDEIYLGQRESPEWT 796

Query: 819 SDAAAIEVFEKFGKEVFEVENRIIERNKNINFKNRTGPVNVPYTLLLPSSTEGLTGRGIP 874
            D   +  F+KFGK++ ++E +II+RN +    NR+GPVN PYTLL P+S  GLTG+GIP
Sbjct: 797 KDKEPLAAFDKFGKKLTDIEKQIIQRNGDNILTNRSGPVNAPYTLLFPTSEGGLTGKGIP 856

BLAST of MC08g2255 vs. ExPASy Swiss-Prot
Match: O22508 (Probable linoleate 9S-lipoxygenase 8 OS=Solanum tuberosum OX=4113 GN=LOX1.8 PE=2 SV=1)

HSP 1 Score: 1144.8 bits (2960), Expect = 0.0e+00
Identity = 556/845 (65.80%), Postives = 672/845 (79.53%), Query Frame = 0

Query: 39  KKIKGKVILMRSNVLDFTEFHSTLLDGFTELLGGGVSLQLISATQASLDS--RGKVGKRA 98
           KK+KG V++M  NVLDFT+  S+L     ++LG  VS QLIS+ Q    +  +GK    A
Sbjct: 17  KKVKGTVVMMNKNVLDFTDLASSLTGKIFDVLGQKVSFQLISSVQGDPTNGLQGKHSNPA 76

Query: 99  FLERWLTSIPPLFAG-ESVFQVNFDLEDNFGLPGAFFIKNGHTSEFFLKSLTLEDVPGFG 158
           +LE  L ++ PL AG E+ F V FD  + FG+PGAF IKN H +EFFLKSLTLEDVP  G
Sbjct: 77  YLENSLFTLTPLTAGSETAFGVTFDWNEEFGVPGAFIIKNMHITEFFLKSLTLEDVPNHG 136

Query: 159 RIHFDCNSWVYPSGRYKKDRIFFANKTFLPSETPNPLRKYREEELLNLRGDGTGERKEWD 218
           ++HF CNSWVYPS  YK DRIFFAN+ +LPSETP  LRKYRE ELL LRGDGTG+R+ WD
Sbjct: 137 KVHFVCNSWVYPSLNYKSDRIFFANQPYLPSETPELLRKYRENELLTLRGDGTGKREAWD 196

Query: 219 RIYDYDVYNDLSDP-----NVRPILGGS-QHPYPRRGRTGRPRARRDSNYETRLSAVLGL 278
           RIYDYD+YNDL +P     NVR  LGGS ++PYPRRGRTGRP  R D   ++R+  +L L
Sbjct: 197 RIYDYDIYNDLGNPDQGKENVRTTLGGSAEYPYPRRGRTGRPPTRTDPKVKSRIPLILSL 256

Query: 279 TIYVPRDENFGHLKMSDFLGFALKSLSSTVQPELVNLINITPGEFDKFQDVHDLYEGGFP 338
            IYVPRDE FGHLKMSDFL +ALKS+   + PEL  L + TP EFD F+DV  LYEGG  
Sbjct: 257 DIYVPRDERFGHLKMSDFLTYALKSIVQFILPELHALFDGTPNEFDSFEDVLRLYEGGIK 316

Query: 339 VPLN-VFRNLTEAFTPPMFQELLRNDGERFLKFSPPQVVRDNKSGWRTDEEFAREMLAGV 398
           +P   +F+ LT A    M +ELLR DGE  L+F  P V++D+K+ WRTDEEFAREMLAGV
Sbjct: 317 LPQGPLFKALTAAIPLEMIRELLRTDGEGILRFPTPLVIKDSKTAWRTDEEFAREMLAGV 376

Query: 399 NPVIICRLKEFPPLSKLDPQIYGDQNSKITEEDIKFGLEGLSADEAIKQNKLYILDHHDA 458
           NP+II RL+EFPP SKLDP+ YG+QNS IT E I+  L+GL+ DEA+  NKL+IL+HHD 
Sbjct: 377 NPIIISRLQEFPPKSKLDPEAYGNQNSTITAEHIEDKLDGLTVDEAMNNNKLFILNHHDV 436

Query: 459 LMPYLRRINSTSTRTYATRTLLFLKADGTLKPLVIELSLPHPQGDQFGANSKLYFPAESG 518
           ++PYLRRIN+T T+TYA+RTLLFL+ +G+LKPL IELSLPHP GDQFG  SK+Y P + G
Sbjct: 437 IIPYLRRINTTITKTYASRTLLFLQDNGSLKPLAIELSLPHPDGDQFGVTSKVYTPTDQG 496

Query: 519 VENSIWQLAKAYVAVNDAGYHQLNSHWLHTHAVQEPFVIATHRQLSVVHPIHKLLVPHFK 578
           VE+SIWQLAKAYVAVND G HQL SHWL+THAV EPFVIAT+RQLSV+HPIHKLL PHF+
Sbjct: 497 VESSIWQLAKAYVAVNDTGVHQLISHWLNTHAVIEPFVIATNRQLSVLHPIHKLLYPHFR 556

Query: 579 DTMFINAFARQVLVNADGLLESTHFQSKYAMELSSDVYKEWNFLEQGLPADLIKRGVAVE 638
           DTM INA ARQ+LVNA G+LEST FQSK+AME+S+ VYK+W F +Q LPADL+KRGVAVE
Sbjct: 557 DTMNINASARQILVNAGGVLESTVFQSKFAMEMSAVVYKDWVFPDQALPADLVKRGVAVE 616

Query: 639 DSNSPHGLRLLIEDYPFAVDGLEIWSTIKTWVTDYISVYYKDDKAVQNDVELQSWWKEVR 698
           DS+SPHG+RLLIEDYP+AVDGLEIWS IK+WVTDY S YY  D+ +  D ELQ+WWKE+R
Sbjct: 617 DSSSPHGVRLLIEDYPYAVDGLEIWSAIKSWVTDYCSFYYGSDEEILKDNELQAWWKELR 676

Query: 699 EKGHADKKNEPWWPKMQTLKELIESCTTIIWISSALHAAVNFGQYPYGGFIPNRPTISRR 758
           E GH DKKNEPWWP+M+T +ELI+SCTTIIWI+SALHAAVNFGQYPY G++PNRPT+SRR
Sbjct: 677 EVGHGDKKNEPWWPEMKTPQELIDSCTTIIWIASALHAAVNFGQYPYAGYLPNRPTVSRR 736

Query: 759 FMPEVGTPEYKELESNPEKAFLRTINSQLQTLLGVSLIEILSRHASDEVYLGQRASIEWT 818
           FMPE GTPEY+EL+ NP+KAFL+TI +QLQTLLGVSL+EILSRH +DE+YLGQR S EWT
Sbjct: 737 FMPEPGTPEYEELKRNPDKAFLKTITAQLQTLLGVSLVEILSRHTTDEIYLGQRESPEWT 796

Query: 819 SDAAAIEVFEKFGKEVFEVENRIIERNKNINFKNRTGPVNVPYTLLLPSSTEGLTGRGIP 874
            D   +  F++FGK++ ++E +II+RN +    NR+GPVN PYTLL P+S  GLTG+GIP
Sbjct: 797 KDKEPLAAFDRFGKKLTDIEKQIIQRNGDNILTNRSGPVNAPYTLLFPTSEGGLTGKGIP 856

BLAST of MC08g2255 vs. NCBI nr
Match: XP_022150773.1 (linoleate 9S-lipoxygenase 6-like [Momordica charantia])

HSP 1 Score: 1768 bits (4579), Expect = 0.0
Identity = 873/873 (100.00%), Postives = 873/873 (100.00%), Query Frame = 0

Query: 1   MFGIGKVIEGALNTTGDLAGSVINAGGNIVDRVTNLGGKKIKGKVILMRSNVLDFTEFHS 60
           MFGIGKVIEGALNTTGDLAGSVINAGGNIVDRVTNLGGKKIKGKVILMRSNVLDFTEFHS
Sbjct: 1   MFGIGKVIEGALNTTGDLAGSVINAGGNIVDRVTNLGGKKIKGKVILMRSNVLDFTEFHS 60

Query: 61  TLLDGFTELLGGGVSLQLISATQASLDSRGKVGKRAFLERWLTSIPPLFAGESVFQVNFD 120
           TLLDGFTELLGGGVSLQLISATQASLDSRGKVGKRAFLERWLTSIPPLFAGESVFQVNFD
Sbjct: 61  TLLDGFTELLGGGVSLQLISATQASLDSRGKVGKRAFLERWLTSIPPLFAGESVFQVNFD 120

Query: 121 LEDNFGLPGAFFIKNGHTSEFFLKSLTLEDVPGFGRIHFDCNSWVYPSGRYKKDRIFFAN 180
           LEDNFGLPGAFFIKNGHTSEFFLKSLTLEDVPGFGRIHFDCNSWVYPSGRYKKDRIFFAN
Sbjct: 121 LEDNFGLPGAFFIKNGHTSEFFLKSLTLEDVPGFGRIHFDCNSWVYPSGRYKKDRIFFAN 180

Query: 181 KTFLPSETPNPLRKYREEELLNLRGDGTGERKEWDRIYDYDVYNDLSDPNVRPILGGSQH 240
           KTFLPSETPNPLRKYREEELLNLRGDGTGERKEWDRIYDYDVYNDLSDPNVRPILGGSQH
Sbjct: 181 KTFLPSETPNPLRKYREEELLNLRGDGTGERKEWDRIYDYDVYNDLSDPNVRPILGGSQH 240

Query: 241 PYPRRGRTGRPRARRDSNYETRLSAVLGLTIYVPRDENFGHLKMSDFLGFALKSLSSTVQ 300
           PYPRRGRTGRPRARRDSNYETRLSAVLGLTIYVPRDENFGHLKMSDFLGFALKSLSSTVQ
Sbjct: 241 PYPRRGRTGRPRARRDSNYETRLSAVLGLTIYVPRDENFGHLKMSDFLGFALKSLSSTVQ 300

Query: 301 PELVNLINITPGEFDKFQDVHDLYEGGFPVPLNVFRNLTEAFTPPMFQELLRNDGERFLK 360
           PELVNLINITPGEFDKFQDVHDLYEGGFPVPLNVFRNLTEAFTPPMFQELLRNDGERFLK
Sbjct: 301 PELVNLINITPGEFDKFQDVHDLYEGGFPVPLNVFRNLTEAFTPPMFQELLRNDGERFLK 360

Query: 361 FSPPQVVRDNKSGWRTDEEFAREMLAGVNPVIICRLKEFPPLSKLDPQIYGDQNSKITEE 420
           FSPPQVVRDNKSGWRTDEEFAREMLAGVNPVIICRLKEFPPLSKLDPQIYGDQNSKITEE
Sbjct: 361 FSPPQVVRDNKSGWRTDEEFAREMLAGVNPVIICRLKEFPPLSKLDPQIYGDQNSKITEE 420

Query: 421 DIKFGLEGLSADEAIKQNKLYILDHHDALMPYLRRINSTSTRTYATRTLLFLKADGTLKP 480
           DIKFGLEGLSADEAIKQNKLYILDHHDALMPYLRRINSTSTRTYATRTLLFLKADGTLKP
Sbjct: 421 DIKFGLEGLSADEAIKQNKLYILDHHDALMPYLRRINSTSTRTYATRTLLFLKADGTLKP 480

Query: 481 LVIELSLPHPQGDQFGANSKLYFPAESGVENSIWQLAKAYVAVNDAGYHQLNSHWLHTHA 540
           LVIELSLPHPQGDQFGANSKLYFPAESGVENSIWQLAKAYVAVNDAGYHQLNSHWLHTHA
Sbjct: 481 LVIELSLPHPQGDQFGANSKLYFPAESGVENSIWQLAKAYVAVNDAGYHQLNSHWLHTHA 540

Query: 541 VQEPFVIATHRQLSVVHPIHKLLVPHFKDTMFINAFARQVLVNADGLLESTHFQSKYAME 600
           VQEPFVIATHRQLSVVHPIHKLLVPHFKDTMFINAFARQVLVNADGLLESTHFQSKYAME
Sbjct: 541 VQEPFVIATHRQLSVVHPIHKLLVPHFKDTMFINAFARQVLVNADGLLESTHFQSKYAME 600

Query: 601 LSSDVYKEWNFLEQGLPADLIKRGVAVEDSNSPHGLRLLIEDYPFAVDGLEIWSTIKTWV 660
           LSSDVYKEWNFLEQGLPADLIKRGVAVEDSNSPHGLRLLIEDYPFAVDGLEIWSTIKTWV
Sbjct: 601 LSSDVYKEWNFLEQGLPADLIKRGVAVEDSNSPHGLRLLIEDYPFAVDGLEIWSTIKTWV 660

Query: 661 TDYISVYYKDDKAVQNDVELQSWWKEVREKGHADKKNEPWWPKMQTLKELIESCTTIIWI 720
           TDYISVYYKDDKAVQNDVELQSWWKEVREKGHADKKNEPWWPKMQTLKELIESCTTIIWI
Sbjct: 661 TDYISVYYKDDKAVQNDVELQSWWKEVREKGHADKKNEPWWPKMQTLKELIESCTTIIWI 720

Query: 721 SSALHAAVNFGQYPYGGFIPNRPTISRRFMPEVGTPEYKELESNPEKAFLRTINSQLQTL 780
           SSALHAAVNFGQYPYGGFIPNRPTISRRFMPEVGTPEYKELESNPEKAFLRTINSQLQTL
Sbjct: 721 SSALHAAVNFGQYPYGGFIPNRPTISRRFMPEVGTPEYKELESNPEKAFLRTINSQLQTL 780

Query: 781 LGVSLIEILSRHASDEVYLGQRASIEWTSDAAAIEVFEKFGKEVFEVENRIIERNKNINF 840
           LGVSLIEILSRHASDEVYLGQRASIEWTSDAAAIEVFEKFGKEVFEVENRIIERNKNINF
Sbjct: 781 LGVSLIEILSRHASDEVYLGQRASIEWTSDAAAIEVFEKFGKEVFEVENRIIERNKNINF 840

Query: 841 KNRTGPVNVPYTLLLPSSTEGLTGRGIPNSISI 873
           KNRTGPVNVPYTLLLPSSTEGLTGRGIPNSISI
Sbjct: 841 KNRTGPVNVPYTLLLPSSTEGLTGRGIPNSISI 873

BLAST of MC08g2255 vs. NCBI nr
Match: XP_023545242.1 (linoleate 9S-lipoxygenase 6-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023545243.1 linoleate 9S-lipoxygenase 6-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1552 bits (4018), Expect = 0.0
Identity = 747/875 (85.37%), Postives = 822/875 (93.94%), Query Frame = 0

Query: 1   MFGIGK-VIEGALNTTGDLAGSVINAGGNIVDRVTNLGGKKIKGKVILMRSNVLDFTEFH 60
           MFGIGK +IEGALNTTGDLAGSVI AGGNI DRVT+LGGKKIKGKVILMRSNVLDFTEFH
Sbjct: 1   MFGIGKNIIEGALNTTGDLAGSVIGAGGNIADRVTSLGGKKIKGKVILMRSNVLDFTEFH 60

Query: 61  STLLDGFTELLGGGVSLQLISATQASLDSRGKVGKRAFLERWLTSIPPLFAGESVFQVNF 120
           S++LD FTELLGGGVSLQLISAT AS DSRGK+G +AFLERW+TS+PPLFAGESVFQVNF
Sbjct: 61  SSVLDSFTELLGGGVSLQLISATHASNDSRGKLGSKAFLERWITSLPPLFAGESVFQVNF 120

Query: 121 DLEDNFGLPGAFFIKNGHTSEFFLKSLTLEDVPGFGRIHFDCNSWVYPSGRYKKDRIFFA 180
             ED+FG PGAFFIKNGHTSEF LKSLTLEDVPGFG +HFDCNSWVYPSGRYKKDRIFFA
Sbjct: 121 TWEDDFGFPGAFFIKNGHTSEFLLKSLTLEDVPGFGTVHFDCNSWVYPSGRYKKDRIFFA 180

Query: 181 NKTFLPSETPNPLRKYREEELLNLRGDGTGERKEWDRIYDYDVYNDLSDPNVRPILGGSQ 240
           NKT+LPSETPNPLRKYREEEL  LRGDGTGERKEWDRIYDYDVYNDLSDP  RPILGG+Q
Sbjct: 181 NKTYLPSETPNPLRKYREEELSTLRGDGTGERKEWDRIYDYDVYNDLSDPGARPILGGNQ 240

Query: 241 HPYPRRGRTGRPRARRDSNYETRLSAVLGLTIYVPRDENFGHLKMSDFLGFALKSLSSTV 300
           +PYPRRGRTGRPR+R+DSNYETRLS++LGLTIYVPRDENFGHLK+SDFLGFALKSL+S V
Sbjct: 241 YPYPRRGRTGRPRSRKDSNYETRLSSILGLTIYVPRDENFGHLKLSDFLGFALKSLASNV 300

Query: 301 QPELVNLINITPGEFDKFQDVHDLYEGGFPVPLNVFRNLTEAFTPPMFQELLRNDGE-RF 360
           QPELVNLIN+TPGEFDKFQDVHDLYEGGFPVP++VFRNLT+ FTPPMFQELLR + + RF
Sbjct: 301 QPELVNLINLTPGEFDKFQDVHDLYEGGFPVPVDVFRNLTKGFTPPMFQELLRTESDQRF 360

Query: 361 LKFSPPQVVRDNKSGWRTDEEFAREMLAGVNPVIICRLKEFPPLSKLDPQIYGDQNSKIT 420
           LKFS P+VV+++K+ W+TDEEFAREMLAGVNPV+I RL+EFPP+SKLDP++YGDQNSKI 
Sbjct: 361 LKFSRPRVVKEDKNAWQTDEEFAREMLAGVNPVMIRRLEEFPPVSKLDPELYGDQNSKIK 420

Query: 421 EEDIKFGLEGLSADEAIKQNKLYILDHHDALMPYLRRINSTSTRTYATRTLLFLKADGTL 480
           EEDIKFGLEGLS +EA+ + +L+ILDHHDALMP+LR+IN TST+TYATRT+L LKADGTL
Sbjct: 421 EEDIKFGLEGLSVEEALNEKRLFILDHHDALMPFLRKINGTSTKTYATRTILLLKADGTL 480

Query: 481 KPLVIELSLPHPQGDQFGANSKLYFPAESGVENSIWQLAKAYVAVNDAGYHQLNSHWLHT 540
           KPLVIELSLPHPQGDQFGA SK YFPAE GV+ SIWQLAKAYV VNDAGYHQL SHWL+T
Sbjct: 481 KPLVIELSLPHPQGDQFGAVSKQYFPAEEGVQKSIWQLAKAYVVVNDAGYHQLISHWLNT 540

Query: 541 HAVQEPFVIATHRQLSVVHPIHKLLVPHFKDTMFINAFARQVLVNADGLLESTHFQSKYA 600
           HAVQEPFVIATHR LSVVHPIHKLLVPH+KDTMFINAFARQVLVN DGLLESTHFQ+KYA
Sbjct: 541 HAVQEPFVIATHRHLSVVHPIHKLLVPHYKDTMFINAFARQVLVNGDGLLESTHFQAKYA 600

Query: 601 MELSSDVYKEWNFLEQGLPADLIKRGVAVEDSNSPHGLRLLIEDYPFAVDGLEIWSTIKT 660
           MELSS +YK+WNF+EQ LPADLIKRGVAV+D++SPHGLRLLIEDYPFAVDGLEIWS IKT
Sbjct: 601 MELSSYIYKDWNFVEQALPADLIKRGVAVQDASSPHGLRLLIEDYPFAVDGLEIWSIIKT 660

Query: 661 WVTDYISVYYKDDKAVQNDVELQSWWKEVREKGHADKKNEPWWPKMQTLKELIESCTTII 720
           WV +Y S+YYKDD+A++NDVELQSWWKE REKGHADKKNE WWPKMQ++ +L+ESCT II
Sbjct: 661 WVANYCSLYYKDDEAIRNDVELQSWWKEAREKGHADKKNEAWWPKMQSINDLVESCTIII 720

Query: 721 WISSALHAAVNFGQYPYGGFIPNRPTISRRFMPEVGTPEYKELESNPEKAFLRTINSQLQ 780
           WISSALHAAVNFGQYPYGG+IPNRPTISRRFMPEVG+ EYKELES PEKAFLRTINSQLQ
Sbjct: 721 WISSALHAAVNFGQYPYGGYIPNRPTISRRFMPEVGSSEYKELESKPEKAFLRTINSQLQ 780

Query: 781 TLLGVSLIEILSRHASDEVYLGQRASIEWTSDAAAIEVFEKFGKEVFEVENRIIERNKNI 840
           TLLGVSLIEILSRHASDEVYLGQRASIEWTSD AA++ FE FGK+VFEVENRI+ERN+++
Sbjct: 781 TLLGVSLIEILSRHASDEVYLGQRASIEWTSDKAALDAFENFGKQVFEVENRIMERNRDV 840

Query: 841 NFKNRTGPVNVPYTLLLPSSTEGLTGRGIPNSISI 873
           N KNR+GPVN+PYTLLLPSSTEGLTGRGIPNSISI
Sbjct: 841 NLKNRSGPVNMPYTLLLPSSTEGLTGRGIPNSISI 875

BLAST of MC08g2255 vs. NCBI nr
Match: XP_022946175.1 (linoleate 9S-lipoxygenase 6-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1550 bits (4012), Expect = 0.0
Identity = 749/877 (85.40%), Postives = 822/877 (93.73%), Query Frame = 0

Query: 1   MFGIGK-VIEGALNTTGDLAGSVINAGGNIVDRVTNLGGKKIKGKVILMRSNVLDFTEFH 60
           MFGIGK +IEGALNTTGDLAGSVI AGGNI DRVT+LGGKKIKGKVILMRSNVLDFTEFH
Sbjct: 1   MFGIGKNIIEGALNTTGDLAGSVIGAGGNIADRVTSLGGKKIKGKVILMRSNVLDFTEFH 60

Query: 61  STLLDGFTELLGGGVSLQLISATQASLDSRGKVGKRAFLERWLTSIPPLFAGESVFQVNF 120
           S++LD FTELLGGGVSLQLISAT AS DSRGK+G +AFLERW+TS+PPLFAGESVFQVNF
Sbjct: 61  SSVLDSFTELLGGGVSLQLISATHASNDSRGKLGSKAFLERWITSLPPLFAGESVFQVNF 120

Query: 121 DLEDNFGLPGAFFIKNGHTSEFFLKSLTLEDVPGFGRIHFDCNSWVYPSGRYKKDRIFFA 180
             ED+FG PGAFFIKNGHTSEFFLKSLTLEDVPGFG +HFDCNSWVYPSGRYKKDRIFFA
Sbjct: 121 TWEDDFGFPGAFFIKNGHTSEFFLKSLTLEDVPGFGTVHFDCNSWVYPSGRYKKDRIFFA 180

Query: 181 NKTFLPSETPNPLRKYREEELLNLRGDGTGERKEWDRIYDYDVYNDLSDP--NVRPILGG 240
           NKT+LPSETPNPLRKYREEEL  LRGDGTGERKEWDRIYDYDVYNDLS+P  + RPILGG
Sbjct: 181 NKTYLPSETPNPLRKYREEELSTLRGDGTGERKEWDRIYDYDVYNDLSEPGKDARPILGG 240

Query: 241 SQHPYPRRGRTGRPRARRDSNYETRLSAVLGLTIYVPRDENFGHLKMSDFLGFALKSLSS 300
           SQ PYPRRGRTGRPR+R+DSNYETRLS+VLGLTIYVPRDENFGHLK+SDFLGFALKSL+S
Sbjct: 241 SQFPYPRRGRTGRPRSRKDSNYETRLSSVLGLTIYVPRDENFGHLKLSDFLGFALKSLAS 300

Query: 301 TVQPELVNLINITPGEFDKFQDVHDLYEGGFPVPLNVFRNLTEAFTPPMFQELLRNDGE- 360
           TVQPELVNLIN+TPGEFDKFQDVHDLYEGGFPVP++VFRNLT+ FTPPMFQELLR + + 
Sbjct: 301 TVQPELVNLINLTPGEFDKFQDVHDLYEGGFPVPVDVFRNLTKGFTPPMFQELLRTESDQ 360

Query: 361 RFLKFSPPQVVRDNKSGWRTDEEFAREMLAGVNPVIICRLKEFPPLSKLDPQIYGDQNSK 420
           RFLKFS P+VV+++K+ W+TDEEFAREMLAGVNPV+I RL+EFPP+SKLDP+ YGDQNSK
Sbjct: 361 RFLKFSRPRVVKEDKNAWQTDEEFAREMLAGVNPVMIRRLEEFPPVSKLDPEFYGDQNSK 420

Query: 421 ITEEDIKFGLEGLSADEAIKQNKLYILDHHDALMPYLRRINSTSTRTYATRTLLFLKADG 480
           I EEDIKFGLEGLS +EA+ + +L+ILDHHDALMP+LR+IN TST+TYATRT+L LKADG
Sbjct: 421 IKEEDIKFGLEGLSVEEALNEKRLFILDHHDALMPFLRKINGTSTKTYATRTILLLKADG 480

Query: 481 TLKPLVIELSLPHPQGDQFGANSKLYFPAESGVENSIWQLAKAYVAVNDAGYHQLNSHWL 540
           TLKPLVIELSLPHPQGDQFGA SK YFPAE GV+ SIWQLAKAYV VNDAGYHQL SHWL
Sbjct: 481 TLKPLVIELSLPHPQGDQFGAVSKQYFPAEEGVQKSIWQLAKAYVVVNDAGYHQLISHWL 540

Query: 541 HTHAVQEPFVIATHRQLSVVHPIHKLLVPHFKDTMFINAFARQVLVNADGLLESTHFQSK 600
           +THAVQEPFVIATHR LSVVHPIHKLLVPH+KDTMFINAFARQVLVN DGLLESTHFQ+K
Sbjct: 541 NTHAVQEPFVIATHRHLSVVHPIHKLLVPHYKDTMFINAFARQVLVNGDGLLESTHFQAK 600

Query: 601 YAMELSSDVYKEWNFLEQGLPADLIKRGVAVEDSNSPHGLRLLIEDYPFAVDGLEIWSTI 660
           YAMELSS +YK+WNF+EQ LP DLIKRGVAV+D+NSPHGLRLLIEDYPFAVDGLEIWS I
Sbjct: 601 YAMELSSYIYKDWNFVEQALPVDLIKRGVAVQDANSPHGLRLLIEDYPFAVDGLEIWSII 660

Query: 661 KTWVTDYISVYYKDDKAVQNDVELQSWWKEVREKGHADKKNEPWWPKMQTLKELIESCTT 720
           KTWV +Y S+YYKDD+A++NDVELQSWWKE REKGHADKKNE WWPKMQ++ +L+ESCT 
Sbjct: 661 KTWVANYCSLYYKDDEAIRNDVELQSWWKEAREKGHADKKNEAWWPKMQSINDLVESCTI 720

Query: 721 IIWISSALHAAVNFGQYPYGGFIPNRPTISRRFMPEVGTPEYKELESNPEKAFLRTINSQ 780
           IIWISSALHAAVNFGQYPYGG+IPNRPTISRRFMPE+G+ EYKELES PEKAFLRTINSQ
Sbjct: 721 IIWISSALHAAVNFGQYPYGGYIPNRPTISRRFMPEIGSSEYKELESKPEKAFLRTINSQ 780

Query: 781 LQTLLGVSLIEILSRHASDEVYLGQRASIEWTSDAAAIEVFEKFGKEVFEVENRIIERNK 840
           LQTLLGVSLIEILSRHASDEVYLGQRASIEWTSD AA++ FE FGK+VFEVENRI+ERN+
Sbjct: 781 LQTLLGVSLIEILSRHASDEVYLGQRASIEWTSDKAALDAFENFGKQVFEVENRIMERNR 840

Query: 841 NINFKNRTGPVNVPYTLLLPSSTEGLTGRGIPNSISI 873
           ++N KNR+GPVN+PYTLLLPSSTEGLTGRGIPNSISI
Sbjct: 841 DVNLKNRSGPVNMPYTLLLPSSTEGLTGRGIPNSISI 877

BLAST of MC08g2255 vs. NCBI nr
Match: XP_022999020.1 (linoleate 9S-lipoxygenase 6-like [Cucurbita maxima] >XP_022999023.1 linoleate 9S-lipoxygenase 6-like [Cucurbita maxima])

HSP 1 Score: 1550 bits (4012), Expect = 0.0
Identity = 749/877 (85.40%), Postives = 823/877 (93.84%), Query Frame = 0

Query: 1   MFGIGK-VIEGALNTTGDLAGSVINAGGNIVDRVTNLGGKKIKGKVILMRSNVLDFTEFH 60
           MFGIGK +IEGALNTTGDLAGSVI AGGNIVDRVTNLGGKKIKGKVILMRSNVLDFTEFH
Sbjct: 1   MFGIGKNIIEGALNTTGDLAGSVIGAGGNIVDRVTNLGGKKIKGKVILMRSNVLDFTEFH 60

Query: 61  STLLDGFTELLGGGVSLQLISATQASLDSRGKVGKRAFLERWLTSIPPLFAGESVFQVNF 120
           S++LD FTELLGGGVSLQLISAT AS DSRGK+G  AFLERWLTS+PPLFAGESVFQVNF
Sbjct: 61  SSVLDSFTELLGGGVSLQLISATHASNDSRGKLGNNAFLERWLTSLPPLFAGESVFQVNF 120

Query: 121 DLEDNFGLPGAFFIKNGHTSEFFLKSLTLEDVPGFGRIHFDCNSWVYPSGRYKKDRIFFA 180
             ED+FG PGAFFIKNGHTSEFFLKSLTLEDVPGFG +HFDCNSWVYPSGRYKKDRIFFA
Sbjct: 121 TWEDDFGFPGAFFIKNGHTSEFFLKSLTLEDVPGFGTVHFDCNSWVYPSGRYKKDRIFFA 180

Query: 181 NKTFLPSETPNPLRKYREEELLNLRGDGTGERKEWDRIYDYDVYNDLSDP--NVRPILGG 240
           NKT+LPSETPNPLRKYREEEL  LRGDGTGERKEWDRIYDYDVYNDLS+P  + RPILGG
Sbjct: 181 NKTYLPSETPNPLRKYREEELSTLRGDGTGERKEWDRIYDYDVYNDLSEPGKDARPILGG 240

Query: 241 SQHPYPRRGRTGRPRARRDSNYETRLSAVLGLTIYVPRDENFGHLKMSDFLGFALKSLSS 300
           SQ PYPRRGRTGRPR+R+DSNYETRLS++LGLTIYVPRDENFGHLK+SDFLGFALKSL+S
Sbjct: 241 SQFPYPRRGRTGRPRSRKDSNYETRLSSILGLTIYVPRDENFGHLKLSDFLGFALKSLAS 300

Query: 301 TVQPELVNLINITPGEFDKFQDVHDLYEGGFPVPLNVFRNLTEAFTPPMFQELLRNDGE- 360
            VQPELVNLIN+TPGEFDKFQDVHDLYEGGFPVP++VFRNLT+ FTPPMFQELLR + + 
Sbjct: 301 NVQPELVNLINLTPGEFDKFQDVHDLYEGGFPVPVDVFRNLTKGFTPPMFQELLRTESDQ 360

Query: 361 RFLKFSPPQVVRDNKSGWRTDEEFAREMLAGVNPVIICRLKEFPPLSKLDPQIYGDQNSK 420
           RFLKFS P+VV+++K+ W+TDEEFAREMLAGVNPV+I RL+EFPP+SKLDP++YGDQNSK
Sbjct: 361 RFLKFSRPRVVKEDKNAWQTDEEFAREMLAGVNPVMIRRLEEFPPVSKLDPELYGDQNSK 420

Query: 421 ITEEDIKFGLEGLSADEAIKQNKLYILDHHDALMPYLRRINSTSTRTYATRTLLFLKADG 480
           I EEDIKFGLEGLS +EA+ + +L+ILDHHD+LMP+LR+IN TST+TYATRT+L LKADG
Sbjct: 421 IKEEDIKFGLEGLSVEEALNEKRLFILDHHDSLMPFLRKINGTSTKTYATRTILLLKADG 480

Query: 481 TLKPLVIELSLPHPQGDQFGANSKLYFPAESGVENSIWQLAKAYVAVNDAGYHQLNSHWL 540
           TLKPLVIELSLPHPQGDQFGA SK YFPAE GV+ SIWQLAKAYV VNDAGYHQL SHWL
Sbjct: 481 TLKPLVIELSLPHPQGDQFGAVSKQYFPAEGGVQKSIWQLAKAYVVVNDAGYHQLISHWL 540

Query: 541 HTHAVQEPFVIATHRQLSVVHPIHKLLVPHFKDTMFINAFARQVLVNADGLLESTHFQSK 600
           +THAVQEPFVIATHR LSVVHPIHKLLVPH+KDTMFINAFARQVLVN DGLLESTHFQ+K
Sbjct: 541 NTHAVQEPFVIATHRHLSVVHPIHKLLVPHYKDTMFINAFARQVLVNGDGLLESTHFQAK 600

Query: 601 YAMELSSDVYKEWNFLEQGLPADLIKRGVAVEDSNSPHGLRLLIEDYPFAVDGLEIWSTI 660
           YA+ELSS +YK+WNF+EQ LPADLIKRGVAV+D+++PHGLRLLIEDYPFAVDGLEIWS I
Sbjct: 601 YALELSSYIYKDWNFVEQALPADLIKRGVAVQDASAPHGLRLLIEDYPFAVDGLEIWSII 660

Query: 661 KTWVTDYISVYYKDDKAVQNDVELQSWWKEVREKGHADKKNEPWWPKMQTLKELIESCTT 720
           KTWV +Y S+YYKDD+A+QNDVELQSWWKE REKGHADKKNE WWPKMQ++ +L+ESCT 
Sbjct: 661 KTWVANYCSLYYKDDEAIQNDVELQSWWKEAREKGHADKKNEAWWPKMQSINDLVESCTI 720

Query: 721 IIWISSALHAAVNFGQYPYGGFIPNRPTISRRFMPEVGTPEYKELESNPEKAFLRTINSQ 780
           IIWISSALHAAVNFGQYPYGG+IPNRPTISRRFMPEVG+ EYKELES PEKAFLRTINSQ
Sbjct: 721 IIWISSALHAAVNFGQYPYGGYIPNRPTISRRFMPEVGSSEYKELESKPEKAFLRTINSQ 780

Query: 781 LQTLLGVSLIEILSRHASDEVYLGQRASIEWTSDAAAIEVFEKFGKEVFEVENRIIERNK 840
           LQTLLGVSLIEILSRHASDEVYLGQRASIEWTSD AA++ FE FGK+VFEVENRI+ERN+
Sbjct: 781 LQTLLGVSLIEILSRHASDEVYLGQRASIEWTSDKAALDAFENFGKQVFEVENRIMERNR 840

Query: 841 NINFKNRTGPVNVPYTLLLPSSTEGLTGRGIPNSISI 873
           ++N KNR+GPVN+PYTLLLPSSTEGLTGRGIPNSISI
Sbjct: 841 DVNLKNRSGPVNMPYTLLLPSSTEGLTGRGIPNSISI 877

BLAST of MC08g2255 vs. NCBI nr
Match: XP_022946176.1 (linoleate 9S-lipoxygenase 6-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1548 bits (4009), Expect = 0.0
Identity = 748/877 (85.29%), Postives = 822/877 (93.73%), Query Frame = 0

Query: 1   MFGIGK-VIEGALNTTGDLAGSVINAGGNIVDRVTNLGGKKIKGKVILMRSNVLDFTEFH 60
           MFGIGK +IEGALNTTGDLAG+VI AGGNI DRVT+LGGKKIKGKVILMRSNVLDFTEFH
Sbjct: 1   MFGIGKNIIEGALNTTGDLAGAVIGAGGNIADRVTSLGGKKIKGKVILMRSNVLDFTEFH 60

Query: 61  STLLDGFTELLGGGVSLQLISATQASLDSRGKVGKRAFLERWLTSIPPLFAGESVFQVNF 120
           S++LD FTELLGGGVSLQLISAT AS DSRGK+G +AFLERW+TS+PPLFAGESVFQVNF
Sbjct: 61  SSVLDSFTELLGGGVSLQLISATHASNDSRGKLGSKAFLERWITSLPPLFAGESVFQVNF 120

Query: 121 DLEDNFGLPGAFFIKNGHTSEFFLKSLTLEDVPGFGRIHFDCNSWVYPSGRYKKDRIFFA 180
             ED+FG PGAFFIKNGHTSEFFLKSLTLEDVPGFG +HFDCNSWVYPSGRYKKDRIFFA
Sbjct: 121 TWEDDFGFPGAFFIKNGHTSEFFLKSLTLEDVPGFGTVHFDCNSWVYPSGRYKKDRIFFA 180

Query: 181 NKTFLPSETPNPLRKYREEELLNLRGDGTGERKEWDRIYDYDVYNDLSDP--NVRPILGG 240
           NKT+LPSETPNPLRKYREEEL  LRGDGTGERKEWDRIYDYDVYNDLS+P  + RPILGG
Sbjct: 181 NKTYLPSETPNPLRKYREEELSTLRGDGTGERKEWDRIYDYDVYNDLSEPGKDARPILGG 240

Query: 241 SQHPYPRRGRTGRPRARRDSNYETRLSAVLGLTIYVPRDENFGHLKMSDFLGFALKSLSS 300
           SQ PYPRRGRTGRPR+R+DSNYETRLS+VLGLTIYVPRDENFGHLK+SDFLGFALKSL+S
Sbjct: 241 SQFPYPRRGRTGRPRSRKDSNYETRLSSVLGLTIYVPRDENFGHLKLSDFLGFALKSLAS 300

Query: 301 TVQPELVNLINITPGEFDKFQDVHDLYEGGFPVPLNVFRNLTEAFTPPMFQELLRNDGE- 360
           TVQPELVNLIN+TPGEFDKFQDVHDLYEGGFPVP++VFRNLT+ FTPPMFQELLR + + 
Sbjct: 301 TVQPELVNLINLTPGEFDKFQDVHDLYEGGFPVPVDVFRNLTKGFTPPMFQELLRTESDQ 360

Query: 361 RFLKFSPPQVVRDNKSGWRTDEEFAREMLAGVNPVIICRLKEFPPLSKLDPQIYGDQNSK 420
           RFLKFS P+VV+++K+ W+TDEEFAREMLAGVNPV+I RL+EFPP+SKLDP+ YGDQNSK
Sbjct: 361 RFLKFSRPRVVKEDKNAWQTDEEFAREMLAGVNPVMIRRLEEFPPVSKLDPEFYGDQNSK 420

Query: 421 ITEEDIKFGLEGLSADEAIKQNKLYILDHHDALMPYLRRINSTSTRTYATRTLLFLKADG 480
           I EEDIKFGLEGLS +EA+ + +L+ILDHHDALMP+LR+IN TST+TYATRT+L LKADG
Sbjct: 421 IKEEDIKFGLEGLSVEEALNEKRLFILDHHDALMPFLRKINGTSTKTYATRTILLLKADG 480

Query: 481 TLKPLVIELSLPHPQGDQFGANSKLYFPAESGVENSIWQLAKAYVAVNDAGYHQLNSHWL 540
           TLKPLVIELSLPHPQGDQFGA SK YFPAE GV+ SIWQLAKAYV VNDAGYHQL SHWL
Sbjct: 481 TLKPLVIELSLPHPQGDQFGAVSKQYFPAEEGVQKSIWQLAKAYVVVNDAGYHQLISHWL 540

Query: 541 HTHAVQEPFVIATHRQLSVVHPIHKLLVPHFKDTMFINAFARQVLVNADGLLESTHFQSK 600
           +THAVQEPFVIATHR LSVVHPIHKLLVPH+KDTMFINAFARQVLVN DGLLESTHFQ+K
Sbjct: 541 NTHAVQEPFVIATHRHLSVVHPIHKLLVPHYKDTMFINAFARQVLVNGDGLLESTHFQAK 600

Query: 601 YAMELSSDVYKEWNFLEQGLPADLIKRGVAVEDSNSPHGLRLLIEDYPFAVDGLEIWSTI 660
           YAMELSS +YK+WNF+EQ LP DLIKRGVAV+D+NSPHGLRLLIEDYPFAVDGLEIWS I
Sbjct: 601 YAMELSSYIYKDWNFVEQALPVDLIKRGVAVQDANSPHGLRLLIEDYPFAVDGLEIWSII 660

Query: 661 KTWVTDYISVYYKDDKAVQNDVELQSWWKEVREKGHADKKNEPWWPKMQTLKELIESCTT 720
           KTWV +Y S+YYKDD+A++NDVELQSWWKE REKGHADKKNE WWPKMQ++ +L+ESCT 
Sbjct: 661 KTWVANYCSLYYKDDEAIRNDVELQSWWKEAREKGHADKKNEAWWPKMQSINDLVESCTI 720

Query: 721 IIWISSALHAAVNFGQYPYGGFIPNRPTISRRFMPEVGTPEYKELESNPEKAFLRTINSQ 780
           IIWISSALHAAVNFGQYPYGG+IPNRPTISRRFMPE+G+ EYKELES PEKAFLRTINSQ
Sbjct: 721 IIWISSALHAAVNFGQYPYGGYIPNRPTISRRFMPEIGSSEYKELESKPEKAFLRTINSQ 780

Query: 781 LQTLLGVSLIEILSRHASDEVYLGQRASIEWTSDAAAIEVFEKFGKEVFEVENRIIERNK 840
           LQTLLGVSLIEILSRHASDEVYLGQRASIEWTSD AA++ FE FGK+VFEVENRI+ERN+
Sbjct: 781 LQTLLGVSLIEILSRHASDEVYLGQRASIEWTSDKAALDAFENFGKQVFEVENRIMERNR 840

Query: 841 NINFKNRTGPVNVPYTLLLPSSTEGLTGRGIPNSISI 873
           ++N KNR+GPVN+PYTLLLPSSTEGLTGRGIPNSISI
Sbjct: 841 DVNLKNRSGPVNMPYTLLLPSSTEGLTGRGIPNSISI 877

BLAST of MC08g2255 vs. ExPASy TrEMBL
Match: A0A6J1DAB9 (Lipoxygenase OS=Momordica charantia OX=3673 GN=LOC111018837 PE=3 SV=1)

HSP 1 Score: 1768 bits (4579), Expect = 0.0
Identity = 873/873 (100.00%), Postives = 873/873 (100.00%), Query Frame = 0

Query: 1   MFGIGKVIEGALNTTGDLAGSVINAGGNIVDRVTNLGGKKIKGKVILMRSNVLDFTEFHS 60
           MFGIGKVIEGALNTTGDLAGSVINAGGNIVDRVTNLGGKKIKGKVILMRSNVLDFTEFHS
Sbjct: 1   MFGIGKVIEGALNTTGDLAGSVINAGGNIVDRVTNLGGKKIKGKVILMRSNVLDFTEFHS 60

Query: 61  TLLDGFTELLGGGVSLQLISATQASLDSRGKVGKRAFLERWLTSIPPLFAGESVFQVNFD 120
           TLLDGFTELLGGGVSLQLISATQASLDSRGKVGKRAFLERWLTSIPPLFAGESVFQVNFD
Sbjct: 61  TLLDGFTELLGGGVSLQLISATQASLDSRGKVGKRAFLERWLTSIPPLFAGESVFQVNFD 120

Query: 121 LEDNFGLPGAFFIKNGHTSEFFLKSLTLEDVPGFGRIHFDCNSWVYPSGRYKKDRIFFAN 180
           LEDNFGLPGAFFIKNGHTSEFFLKSLTLEDVPGFGRIHFDCNSWVYPSGRYKKDRIFFAN
Sbjct: 121 LEDNFGLPGAFFIKNGHTSEFFLKSLTLEDVPGFGRIHFDCNSWVYPSGRYKKDRIFFAN 180

Query: 181 KTFLPSETPNPLRKYREEELLNLRGDGTGERKEWDRIYDYDVYNDLSDPNVRPILGGSQH 240
           KTFLPSETPNPLRKYREEELLNLRGDGTGERKEWDRIYDYDVYNDLSDPNVRPILGGSQH
Sbjct: 181 KTFLPSETPNPLRKYREEELLNLRGDGTGERKEWDRIYDYDVYNDLSDPNVRPILGGSQH 240

Query: 241 PYPRRGRTGRPRARRDSNYETRLSAVLGLTIYVPRDENFGHLKMSDFLGFALKSLSSTVQ 300
           PYPRRGRTGRPRARRDSNYETRLSAVLGLTIYVPRDENFGHLKMSDFLGFALKSLSSTVQ
Sbjct: 241 PYPRRGRTGRPRARRDSNYETRLSAVLGLTIYVPRDENFGHLKMSDFLGFALKSLSSTVQ 300

Query: 301 PELVNLINITPGEFDKFQDVHDLYEGGFPVPLNVFRNLTEAFTPPMFQELLRNDGERFLK 360
           PELVNLINITPGEFDKFQDVHDLYEGGFPVPLNVFRNLTEAFTPPMFQELLRNDGERFLK
Sbjct: 301 PELVNLINITPGEFDKFQDVHDLYEGGFPVPLNVFRNLTEAFTPPMFQELLRNDGERFLK 360

Query: 361 FSPPQVVRDNKSGWRTDEEFAREMLAGVNPVIICRLKEFPPLSKLDPQIYGDQNSKITEE 420
           FSPPQVVRDNKSGWRTDEEFAREMLAGVNPVIICRLKEFPPLSKLDPQIYGDQNSKITEE
Sbjct: 361 FSPPQVVRDNKSGWRTDEEFAREMLAGVNPVIICRLKEFPPLSKLDPQIYGDQNSKITEE 420

Query: 421 DIKFGLEGLSADEAIKQNKLYILDHHDALMPYLRRINSTSTRTYATRTLLFLKADGTLKP 480
           DIKFGLEGLSADEAIKQNKLYILDHHDALMPYLRRINSTSTRTYATRTLLFLKADGTLKP
Sbjct: 421 DIKFGLEGLSADEAIKQNKLYILDHHDALMPYLRRINSTSTRTYATRTLLFLKADGTLKP 480

Query: 481 LVIELSLPHPQGDQFGANSKLYFPAESGVENSIWQLAKAYVAVNDAGYHQLNSHWLHTHA 540
           LVIELSLPHPQGDQFGANSKLYFPAESGVENSIWQLAKAYVAVNDAGYHQLNSHWLHTHA
Sbjct: 481 LVIELSLPHPQGDQFGANSKLYFPAESGVENSIWQLAKAYVAVNDAGYHQLNSHWLHTHA 540

Query: 541 VQEPFVIATHRQLSVVHPIHKLLVPHFKDTMFINAFARQVLVNADGLLESTHFQSKYAME 600
           VQEPFVIATHRQLSVVHPIHKLLVPHFKDTMFINAFARQVLVNADGLLESTHFQSKYAME
Sbjct: 541 VQEPFVIATHRQLSVVHPIHKLLVPHFKDTMFINAFARQVLVNADGLLESTHFQSKYAME 600

Query: 601 LSSDVYKEWNFLEQGLPADLIKRGVAVEDSNSPHGLRLLIEDYPFAVDGLEIWSTIKTWV 660
           LSSDVYKEWNFLEQGLPADLIKRGVAVEDSNSPHGLRLLIEDYPFAVDGLEIWSTIKTWV
Sbjct: 601 LSSDVYKEWNFLEQGLPADLIKRGVAVEDSNSPHGLRLLIEDYPFAVDGLEIWSTIKTWV 660

Query: 661 TDYISVYYKDDKAVQNDVELQSWWKEVREKGHADKKNEPWWPKMQTLKELIESCTTIIWI 720
           TDYISVYYKDDKAVQNDVELQSWWKEVREKGHADKKNEPWWPKMQTLKELIESCTTIIWI
Sbjct: 661 TDYISVYYKDDKAVQNDVELQSWWKEVREKGHADKKNEPWWPKMQTLKELIESCTTIIWI 720

Query: 721 SSALHAAVNFGQYPYGGFIPNRPTISRRFMPEVGTPEYKELESNPEKAFLRTINSQLQTL 780
           SSALHAAVNFGQYPYGGFIPNRPTISRRFMPEVGTPEYKELESNPEKAFLRTINSQLQTL
Sbjct: 721 SSALHAAVNFGQYPYGGFIPNRPTISRRFMPEVGTPEYKELESNPEKAFLRTINSQLQTL 780

Query: 781 LGVSLIEILSRHASDEVYLGQRASIEWTSDAAAIEVFEKFGKEVFEVENRIIERNKNINF 840
           LGVSLIEILSRHASDEVYLGQRASIEWTSDAAAIEVFEKFGKEVFEVENRIIERNKNINF
Sbjct: 781 LGVSLIEILSRHASDEVYLGQRASIEWTSDAAAIEVFEKFGKEVFEVENRIIERNKNINF 840

Query: 841 KNRTGPVNVPYTLLLPSSTEGLTGRGIPNSISI 873
           KNRTGPVNVPYTLLLPSSTEGLTGRGIPNSISI
Sbjct: 841 KNRTGPVNVPYTLLLPSSTEGLTGRGIPNSISI 873

BLAST of MC08g2255 vs. ExPASy TrEMBL
Match: A0A6J1KIF6 (Lipoxygenase OS=Cucurbita maxima OX=3661 GN=LOC111493538 PE=3 SV=1)

HSP 1 Score: 1550 bits (4012), Expect = 0.0
Identity = 749/877 (85.40%), Postives = 823/877 (93.84%), Query Frame = 0

Query: 1   MFGIGK-VIEGALNTTGDLAGSVINAGGNIVDRVTNLGGKKIKGKVILMRSNVLDFTEFH 60
           MFGIGK +IEGALNTTGDLAGSVI AGGNIVDRVTNLGGKKIKGKVILMRSNVLDFTEFH
Sbjct: 1   MFGIGKNIIEGALNTTGDLAGSVIGAGGNIVDRVTNLGGKKIKGKVILMRSNVLDFTEFH 60

Query: 61  STLLDGFTELLGGGVSLQLISATQASLDSRGKVGKRAFLERWLTSIPPLFAGESVFQVNF 120
           S++LD FTELLGGGVSLQLISAT AS DSRGK+G  AFLERWLTS+PPLFAGESVFQVNF
Sbjct: 61  SSVLDSFTELLGGGVSLQLISATHASNDSRGKLGNNAFLERWLTSLPPLFAGESVFQVNF 120

Query: 121 DLEDNFGLPGAFFIKNGHTSEFFLKSLTLEDVPGFGRIHFDCNSWVYPSGRYKKDRIFFA 180
             ED+FG PGAFFIKNGHTSEFFLKSLTLEDVPGFG +HFDCNSWVYPSGRYKKDRIFFA
Sbjct: 121 TWEDDFGFPGAFFIKNGHTSEFFLKSLTLEDVPGFGTVHFDCNSWVYPSGRYKKDRIFFA 180

Query: 181 NKTFLPSETPNPLRKYREEELLNLRGDGTGERKEWDRIYDYDVYNDLSDP--NVRPILGG 240
           NKT+LPSETPNPLRKYREEEL  LRGDGTGERKEWDRIYDYDVYNDLS+P  + RPILGG
Sbjct: 181 NKTYLPSETPNPLRKYREEELSTLRGDGTGERKEWDRIYDYDVYNDLSEPGKDARPILGG 240

Query: 241 SQHPYPRRGRTGRPRARRDSNYETRLSAVLGLTIYVPRDENFGHLKMSDFLGFALKSLSS 300
           SQ PYPRRGRTGRPR+R+DSNYETRLS++LGLTIYVPRDENFGHLK+SDFLGFALKSL+S
Sbjct: 241 SQFPYPRRGRTGRPRSRKDSNYETRLSSILGLTIYVPRDENFGHLKLSDFLGFALKSLAS 300

Query: 301 TVQPELVNLINITPGEFDKFQDVHDLYEGGFPVPLNVFRNLTEAFTPPMFQELLRNDGE- 360
            VQPELVNLIN+TPGEFDKFQDVHDLYEGGFPVP++VFRNLT+ FTPPMFQELLR + + 
Sbjct: 301 NVQPELVNLINLTPGEFDKFQDVHDLYEGGFPVPVDVFRNLTKGFTPPMFQELLRTESDQ 360

Query: 361 RFLKFSPPQVVRDNKSGWRTDEEFAREMLAGVNPVIICRLKEFPPLSKLDPQIYGDQNSK 420
           RFLKFS P+VV+++K+ W+TDEEFAREMLAGVNPV+I RL+EFPP+SKLDP++YGDQNSK
Sbjct: 361 RFLKFSRPRVVKEDKNAWQTDEEFAREMLAGVNPVMIRRLEEFPPVSKLDPELYGDQNSK 420

Query: 421 ITEEDIKFGLEGLSADEAIKQNKLYILDHHDALMPYLRRINSTSTRTYATRTLLFLKADG 480
           I EEDIKFGLEGLS +EA+ + +L+ILDHHD+LMP+LR+IN TST+TYATRT+L LKADG
Sbjct: 421 IKEEDIKFGLEGLSVEEALNEKRLFILDHHDSLMPFLRKINGTSTKTYATRTILLLKADG 480

Query: 481 TLKPLVIELSLPHPQGDQFGANSKLYFPAESGVENSIWQLAKAYVAVNDAGYHQLNSHWL 540
           TLKPLVIELSLPHPQGDQFGA SK YFPAE GV+ SIWQLAKAYV VNDAGYHQL SHWL
Sbjct: 481 TLKPLVIELSLPHPQGDQFGAVSKQYFPAEGGVQKSIWQLAKAYVVVNDAGYHQLISHWL 540

Query: 541 HTHAVQEPFVIATHRQLSVVHPIHKLLVPHFKDTMFINAFARQVLVNADGLLESTHFQSK 600
           +THAVQEPFVIATHR LSVVHPIHKLLVPH+KDTMFINAFARQVLVN DGLLESTHFQ+K
Sbjct: 541 NTHAVQEPFVIATHRHLSVVHPIHKLLVPHYKDTMFINAFARQVLVNGDGLLESTHFQAK 600

Query: 601 YAMELSSDVYKEWNFLEQGLPADLIKRGVAVEDSNSPHGLRLLIEDYPFAVDGLEIWSTI 660
           YA+ELSS +YK+WNF+EQ LPADLIKRGVAV+D+++PHGLRLLIEDYPFAVDGLEIWS I
Sbjct: 601 YALELSSYIYKDWNFVEQALPADLIKRGVAVQDASAPHGLRLLIEDYPFAVDGLEIWSII 660

Query: 661 KTWVTDYISVYYKDDKAVQNDVELQSWWKEVREKGHADKKNEPWWPKMQTLKELIESCTT 720
           KTWV +Y S+YYKDD+A+QNDVELQSWWKE REKGHADKKNE WWPKMQ++ +L+ESCT 
Sbjct: 661 KTWVANYCSLYYKDDEAIQNDVELQSWWKEAREKGHADKKNEAWWPKMQSINDLVESCTI 720

Query: 721 IIWISSALHAAVNFGQYPYGGFIPNRPTISRRFMPEVGTPEYKELESNPEKAFLRTINSQ 780
           IIWISSALHAAVNFGQYPYGG+IPNRPTISRRFMPEVG+ EYKELES PEKAFLRTINSQ
Sbjct: 721 IIWISSALHAAVNFGQYPYGGYIPNRPTISRRFMPEVGSSEYKELESKPEKAFLRTINSQ 780

Query: 781 LQTLLGVSLIEILSRHASDEVYLGQRASIEWTSDAAAIEVFEKFGKEVFEVENRIIERNK 840
           LQTLLGVSLIEILSRHASDEVYLGQRASIEWTSD AA++ FE FGK+VFEVENRI+ERN+
Sbjct: 781 LQTLLGVSLIEILSRHASDEVYLGQRASIEWTSDKAALDAFENFGKQVFEVENRIMERNR 840

Query: 841 NINFKNRTGPVNVPYTLLLPSSTEGLTGRGIPNSISI 873
           ++N KNR+GPVN+PYTLLLPSSTEGLTGRGIPNSISI
Sbjct: 841 DVNLKNRSGPVNMPYTLLLPSSTEGLTGRGIPNSISI 877

BLAST of MC08g2255 vs. ExPASy TrEMBL
Match: A0A6J1G329 (Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111450337 PE=3 SV=1)

HSP 1 Score: 1550 bits (4012), Expect = 0.0
Identity = 749/877 (85.40%), Postives = 822/877 (93.73%), Query Frame = 0

Query: 1   MFGIGK-VIEGALNTTGDLAGSVINAGGNIVDRVTNLGGKKIKGKVILMRSNVLDFTEFH 60
           MFGIGK +IEGALNTTGDLAGSVI AGGNI DRVT+LGGKKIKGKVILMRSNVLDFTEFH
Sbjct: 1   MFGIGKNIIEGALNTTGDLAGSVIGAGGNIADRVTSLGGKKIKGKVILMRSNVLDFTEFH 60

Query: 61  STLLDGFTELLGGGVSLQLISATQASLDSRGKVGKRAFLERWLTSIPPLFAGESVFQVNF 120
           S++LD FTELLGGGVSLQLISAT AS DSRGK+G +AFLERW+TS+PPLFAGESVFQVNF
Sbjct: 61  SSVLDSFTELLGGGVSLQLISATHASNDSRGKLGSKAFLERWITSLPPLFAGESVFQVNF 120

Query: 121 DLEDNFGLPGAFFIKNGHTSEFFLKSLTLEDVPGFGRIHFDCNSWVYPSGRYKKDRIFFA 180
             ED+FG PGAFFIKNGHTSEFFLKSLTLEDVPGFG +HFDCNSWVYPSGRYKKDRIFFA
Sbjct: 121 TWEDDFGFPGAFFIKNGHTSEFFLKSLTLEDVPGFGTVHFDCNSWVYPSGRYKKDRIFFA 180

Query: 181 NKTFLPSETPNPLRKYREEELLNLRGDGTGERKEWDRIYDYDVYNDLSDP--NVRPILGG 240
           NKT+LPSETPNPLRKYREEEL  LRGDGTGERKEWDRIYDYDVYNDLS+P  + RPILGG
Sbjct: 181 NKTYLPSETPNPLRKYREEELSTLRGDGTGERKEWDRIYDYDVYNDLSEPGKDARPILGG 240

Query: 241 SQHPYPRRGRTGRPRARRDSNYETRLSAVLGLTIYVPRDENFGHLKMSDFLGFALKSLSS 300
           SQ PYPRRGRTGRPR+R+DSNYETRLS+VLGLTIYVPRDENFGHLK+SDFLGFALKSL+S
Sbjct: 241 SQFPYPRRGRTGRPRSRKDSNYETRLSSVLGLTIYVPRDENFGHLKLSDFLGFALKSLAS 300

Query: 301 TVQPELVNLINITPGEFDKFQDVHDLYEGGFPVPLNVFRNLTEAFTPPMFQELLRNDGE- 360
           TVQPELVNLIN+TPGEFDKFQDVHDLYEGGFPVP++VFRNLT+ FTPPMFQELLR + + 
Sbjct: 301 TVQPELVNLINLTPGEFDKFQDVHDLYEGGFPVPVDVFRNLTKGFTPPMFQELLRTESDQ 360

Query: 361 RFLKFSPPQVVRDNKSGWRTDEEFAREMLAGVNPVIICRLKEFPPLSKLDPQIYGDQNSK 420
           RFLKFS P+VV+++K+ W+TDEEFAREMLAGVNPV+I RL+EFPP+SKLDP+ YGDQNSK
Sbjct: 361 RFLKFSRPRVVKEDKNAWQTDEEFAREMLAGVNPVMIRRLEEFPPVSKLDPEFYGDQNSK 420

Query: 421 ITEEDIKFGLEGLSADEAIKQNKLYILDHHDALMPYLRRINSTSTRTYATRTLLFLKADG 480
           I EEDIKFGLEGLS +EA+ + +L+ILDHHDALMP+LR+IN TST+TYATRT+L LKADG
Sbjct: 421 IKEEDIKFGLEGLSVEEALNEKRLFILDHHDALMPFLRKINGTSTKTYATRTILLLKADG 480

Query: 481 TLKPLVIELSLPHPQGDQFGANSKLYFPAESGVENSIWQLAKAYVAVNDAGYHQLNSHWL 540
           TLKPLVIELSLPHPQGDQFGA SK YFPAE GV+ SIWQLAKAYV VNDAGYHQL SHWL
Sbjct: 481 TLKPLVIELSLPHPQGDQFGAVSKQYFPAEEGVQKSIWQLAKAYVVVNDAGYHQLISHWL 540

Query: 541 HTHAVQEPFVIATHRQLSVVHPIHKLLVPHFKDTMFINAFARQVLVNADGLLESTHFQSK 600
           +THAVQEPFVIATHR LSVVHPIHKLLVPH+KDTMFINAFARQVLVN DGLLESTHFQ+K
Sbjct: 541 NTHAVQEPFVIATHRHLSVVHPIHKLLVPHYKDTMFINAFARQVLVNGDGLLESTHFQAK 600

Query: 601 YAMELSSDVYKEWNFLEQGLPADLIKRGVAVEDSNSPHGLRLLIEDYPFAVDGLEIWSTI 660
           YAMELSS +YK+WNF+EQ LP DLIKRGVAV+D+NSPHGLRLLIEDYPFAVDGLEIWS I
Sbjct: 601 YAMELSSYIYKDWNFVEQALPVDLIKRGVAVQDANSPHGLRLLIEDYPFAVDGLEIWSII 660

Query: 661 KTWVTDYISVYYKDDKAVQNDVELQSWWKEVREKGHADKKNEPWWPKMQTLKELIESCTT 720
           KTWV +Y S+YYKDD+A++NDVELQSWWKE REKGHADKKNE WWPKMQ++ +L+ESCT 
Sbjct: 661 KTWVANYCSLYYKDDEAIRNDVELQSWWKEAREKGHADKKNEAWWPKMQSINDLVESCTI 720

Query: 721 IIWISSALHAAVNFGQYPYGGFIPNRPTISRRFMPEVGTPEYKELESNPEKAFLRTINSQ 780
           IIWISSALHAAVNFGQYPYGG+IPNRPTISRRFMPE+G+ EYKELES PEKAFLRTINSQ
Sbjct: 721 IIWISSALHAAVNFGQYPYGGYIPNRPTISRRFMPEIGSSEYKELESKPEKAFLRTINSQ 780

Query: 781 LQTLLGVSLIEILSRHASDEVYLGQRASIEWTSDAAAIEVFEKFGKEVFEVENRIIERNK 840
           LQTLLGVSLIEILSRHASDEVYLGQRASIEWTSD AA++ FE FGK+VFEVENRI+ERN+
Sbjct: 781 LQTLLGVSLIEILSRHASDEVYLGQRASIEWTSDKAALDAFENFGKQVFEVENRIMERNR 840

Query: 841 NINFKNRTGPVNVPYTLLLPSSTEGLTGRGIPNSISI 873
           ++N KNR+GPVN+PYTLLLPSSTEGLTGRGIPNSISI
Sbjct: 841 DVNLKNRSGPVNMPYTLLLPSSTEGLTGRGIPNSISI 877

BLAST of MC08g2255 vs. ExPASy TrEMBL
Match: A0A6J1G2X4 (Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111450337 PE=3 SV=1)

HSP 1 Score: 1548 bits (4009), Expect = 0.0
Identity = 748/877 (85.29%), Postives = 822/877 (93.73%), Query Frame = 0

Query: 1   MFGIGK-VIEGALNTTGDLAGSVINAGGNIVDRVTNLGGKKIKGKVILMRSNVLDFTEFH 60
           MFGIGK +IEGALNTTGDLAG+VI AGGNI DRVT+LGGKKIKGKVILMRSNVLDFTEFH
Sbjct: 1   MFGIGKNIIEGALNTTGDLAGAVIGAGGNIADRVTSLGGKKIKGKVILMRSNVLDFTEFH 60

Query: 61  STLLDGFTELLGGGVSLQLISATQASLDSRGKVGKRAFLERWLTSIPPLFAGESVFQVNF 120
           S++LD FTELLGGGVSLQLISAT AS DSRGK+G +AFLERW+TS+PPLFAGESVFQVNF
Sbjct: 61  SSVLDSFTELLGGGVSLQLISATHASNDSRGKLGSKAFLERWITSLPPLFAGESVFQVNF 120

Query: 121 DLEDNFGLPGAFFIKNGHTSEFFLKSLTLEDVPGFGRIHFDCNSWVYPSGRYKKDRIFFA 180
             ED+FG PGAFFIKNGHTSEFFLKSLTLEDVPGFG +HFDCNSWVYPSGRYKKDRIFFA
Sbjct: 121 TWEDDFGFPGAFFIKNGHTSEFFLKSLTLEDVPGFGTVHFDCNSWVYPSGRYKKDRIFFA 180

Query: 181 NKTFLPSETPNPLRKYREEELLNLRGDGTGERKEWDRIYDYDVYNDLSDP--NVRPILGG 240
           NKT+LPSETPNPLRKYREEEL  LRGDGTGERKEWDRIYDYDVYNDLS+P  + RPILGG
Sbjct: 181 NKTYLPSETPNPLRKYREEELSTLRGDGTGERKEWDRIYDYDVYNDLSEPGKDARPILGG 240

Query: 241 SQHPYPRRGRTGRPRARRDSNYETRLSAVLGLTIYVPRDENFGHLKMSDFLGFALKSLSS 300
           SQ PYPRRGRTGRPR+R+DSNYETRLS+VLGLTIYVPRDENFGHLK+SDFLGFALKSL+S
Sbjct: 241 SQFPYPRRGRTGRPRSRKDSNYETRLSSVLGLTIYVPRDENFGHLKLSDFLGFALKSLAS 300

Query: 301 TVQPELVNLINITPGEFDKFQDVHDLYEGGFPVPLNVFRNLTEAFTPPMFQELLRNDGE- 360
           TVQPELVNLIN+TPGEFDKFQDVHDLYEGGFPVP++VFRNLT+ FTPPMFQELLR + + 
Sbjct: 301 TVQPELVNLINLTPGEFDKFQDVHDLYEGGFPVPVDVFRNLTKGFTPPMFQELLRTESDQ 360

Query: 361 RFLKFSPPQVVRDNKSGWRTDEEFAREMLAGVNPVIICRLKEFPPLSKLDPQIYGDQNSK 420
           RFLKFS P+VV+++K+ W+TDEEFAREMLAGVNPV+I RL+EFPP+SKLDP+ YGDQNSK
Sbjct: 361 RFLKFSRPRVVKEDKNAWQTDEEFAREMLAGVNPVMIRRLEEFPPVSKLDPEFYGDQNSK 420

Query: 421 ITEEDIKFGLEGLSADEAIKQNKLYILDHHDALMPYLRRINSTSTRTYATRTLLFLKADG 480
           I EEDIKFGLEGLS +EA+ + +L+ILDHHDALMP+LR+IN TST+TYATRT+L LKADG
Sbjct: 421 IKEEDIKFGLEGLSVEEALNEKRLFILDHHDALMPFLRKINGTSTKTYATRTILLLKADG 480

Query: 481 TLKPLVIELSLPHPQGDQFGANSKLYFPAESGVENSIWQLAKAYVAVNDAGYHQLNSHWL 540
           TLKPLVIELSLPHPQGDQFGA SK YFPAE GV+ SIWQLAKAYV VNDAGYHQL SHWL
Sbjct: 481 TLKPLVIELSLPHPQGDQFGAVSKQYFPAEEGVQKSIWQLAKAYVVVNDAGYHQLISHWL 540

Query: 541 HTHAVQEPFVIATHRQLSVVHPIHKLLVPHFKDTMFINAFARQVLVNADGLLESTHFQSK 600
           +THAVQEPFVIATHR LSVVHPIHKLLVPH+KDTMFINAFARQVLVN DGLLESTHFQ+K
Sbjct: 541 NTHAVQEPFVIATHRHLSVVHPIHKLLVPHYKDTMFINAFARQVLVNGDGLLESTHFQAK 600

Query: 601 YAMELSSDVYKEWNFLEQGLPADLIKRGVAVEDSNSPHGLRLLIEDYPFAVDGLEIWSTI 660
           YAMELSS +YK+WNF+EQ LP DLIKRGVAV+D+NSPHGLRLLIEDYPFAVDGLEIWS I
Sbjct: 601 YAMELSSYIYKDWNFVEQALPVDLIKRGVAVQDANSPHGLRLLIEDYPFAVDGLEIWSII 660

Query: 661 KTWVTDYISVYYKDDKAVQNDVELQSWWKEVREKGHADKKNEPWWPKMQTLKELIESCTT 720
           KTWV +Y S+YYKDD+A++NDVELQSWWKE REKGHADKKNE WWPKMQ++ +L+ESCT 
Sbjct: 661 KTWVANYCSLYYKDDEAIRNDVELQSWWKEAREKGHADKKNEAWWPKMQSINDLVESCTI 720

Query: 721 IIWISSALHAAVNFGQYPYGGFIPNRPTISRRFMPEVGTPEYKELESNPEKAFLRTINSQ 780
           IIWISSALHAAVNFGQYPYGG+IPNRPTISRRFMPE+G+ EYKELES PEKAFLRTINSQ
Sbjct: 721 IIWISSALHAAVNFGQYPYGGYIPNRPTISRRFMPEIGSSEYKELESKPEKAFLRTINSQ 780

Query: 781 LQTLLGVSLIEILSRHASDEVYLGQRASIEWTSDAAAIEVFEKFGKEVFEVENRIIERNK 840
           LQTLLGVSLIEILSRHASDEVYLGQRASIEWTSD AA++ FE FGK+VFEVENRI+ERN+
Sbjct: 781 LQTLLGVSLIEILSRHASDEVYLGQRASIEWTSDKAALDAFENFGKQVFEVENRIMERNR 840

Query: 841 NINFKNRTGPVNVPYTLLLPSSTEGLTGRGIPNSISI 873
           ++N KNR+GPVN+PYTLLLPSSTEGLTGRGIPNSISI
Sbjct: 841 DVNLKNRSGPVNMPYTLLLPSSTEGLTGRGIPNSISI 877

BLAST of MC08g2255 vs. ExPASy TrEMBL
Match: A0A6J1KBU1 (Lipoxygenase OS=Cucurbita maxima OX=3661 GN=LOC111493537 PE=3 SV=1)

HSP 1 Score: 1548 bits (4007), Expect = 0.0
Identity = 748/877 (85.29%), Postives = 822/877 (93.73%), Query Frame = 0

Query: 1   MFGIGK-VIEGALNTTGDLAGSVINAGGNIVDRVTNLGGKKIKGKVILMRSNVLDFTEFH 60
           MFGIGK +IEGALNTTGDLAGSVI AGGNIVDRVTNLGGKKIKGKVILMRSNVLDFTEFH
Sbjct: 1   MFGIGKNIIEGALNTTGDLAGSVIGAGGNIVDRVTNLGGKKIKGKVILMRSNVLDFTEFH 60

Query: 61  STLLDGFTELLGGGVSLQLISATQASLDSRGKVGKRAFLERWLTSIPPLFAGESVFQVNF 120
           S++LD FTELLGGGVSLQLISAT AS DSRGK+G  AFLERWLTS+PPLFAGESVFQVNF
Sbjct: 61  SSVLDSFTELLGGGVSLQLISATHASNDSRGKLGNNAFLERWLTSLPPLFAGESVFQVNF 120

Query: 121 DLEDNFGLPGAFFIKNGHTSEFFLKSLTLEDVPGFGRIHFDCNSWVYPSGRYKKDRIFFA 180
             ED+FG PGAFFIKNGHTSEFFLKSL LEDVPGFG +HFDCNSWVYPSGRYKKDRIFFA
Sbjct: 121 TWEDDFGFPGAFFIKNGHTSEFFLKSLALEDVPGFGTVHFDCNSWVYPSGRYKKDRIFFA 180

Query: 181 NKTFLPSETPNPLRKYREEELLNLRGDGTGERKEWDRIYDYDVYNDLSDP--NVRPILGG 240
           NKT+LPSETPNPLRKYREEEL  LRGDGTGERKEWDRIYDYDVYNDLS+P  + RPILGG
Sbjct: 181 NKTYLPSETPNPLRKYREEELSTLRGDGTGERKEWDRIYDYDVYNDLSEPGKDARPILGG 240

Query: 241 SQHPYPRRGRTGRPRARRDSNYETRLSAVLGLTIYVPRDENFGHLKMSDFLGFALKSLSS 300
           SQ PYPRRGRTGRPR+R+DSNYETRLS++LGLTIYVPRDENFGHLK+SDFLGFALKSL+S
Sbjct: 241 SQFPYPRRGRTGRPRSRKDSNYETRLSSILGLTIYVPRDENFGHLKLSDFLGFALKSLAS 300

Query: 301 TVQPELVNLINITPGEFDKFQDVHDLYEGGFPVPLNVFRNLTEAFTPPMFQELLRNDGE- 360
            VQPELVNLIN+TPGEFDKFQDVHDLYEGGFPVP++VFRNLT+ FTPPMFQELLR + + 
Sbjct: 301 NVQPELVNLINLTPGEFDKFQDVHDLYEGGFPVPVDVFRNLTKGFTPPMFQELLRTESDQ 360

Query: 361 RFLKFSPPQVVRDNKSGWRTDEEFAREMLAGVNPVIICRLKEFPPLSKLDPQIYGDQNSK 420
           RFLKFS P+VV+++K+ W+TDEEFAREMLAGVNPV+I RL+EFPP+SKLDP++YGDQNSK
Sbjct: 361 RFLKFSRPRVVKEDKNAWQTDEEFAREMLAGVNPVMIRRLEEFPPVSKLDPELYGDQNSK 420

Query: 421 ITEEDIKFGLEGLSADEAIKQNKLYILDHHDALMPYLRRINSTSTRTYATRTLLFLKADG 480
           I EEDIKFGLEGLS +EA+ + +L+ILDHHD+LMP+LR+IN TST+TYATRT+L LKADG
Sbjct: 421 IKEEDIKFGLEGLSVEEALNEKRLFILDHHDSLMPFLRKINGTSTKTYATRTILLLKADG 480

Query: 481 TLKPLVIELSLPHPQGDQFGANSKLYFPAESGVENSIWQLAKAYVAVNDAGYHQLNSHWL 540
           TLKPLVIELSLPHPQGDQFGA SK YFPAE GV+ SIWQLAKAYV VNDAGYHQL SHWL
Sbjct: 481 TLKPLVIELSLPHPQGDQFGAVSKQYFPAEGGVQKSIWQLAKAYVVVNDAGYHQLISHWL 540

Query: 541 HTHAVQEPFVIATHRQLSVVHPIHKLLVPHFKDTMFINAFARQVLVNADGLLESTHFQSK 600
           +THAVQEPFVIATHR LSVVHPIHKLLVPH+KDTMFINAFARQVLVN DGLLESTHFQ+K
Sbjct: 541 NTHAVQEPFVIATHRHLSVVHPIHKLLVPHYKDTMFINAFARQVLVNGDGLLESTHFQAK 600

Query: 601 YAMELSSDVYKEWNFLEQGLPADLIKRGVAVEDSNSPHGLRLLIEDYPFAVDGLEIWSTI 660
           YA+ELSS +YK+WNF+EQ LPADLIKRGVAV+D+++PHGLRLLIEDYPFAVDGLEIWS I
Sbjct: 601 YALELSSYIYKDWNFVEQALPADLIKRGVAVQDASAPHGLRLLIEDYPFAVDGLEIWSII 660

Query: 661 KTWVTDYISVYYKDDKAVQNDVELQSWWKEVREKGHADKKNEPWWPKMQTLKELIESCTT 720
           KTWV +Y S+YYKDD+A+QNDVELQSWWKE REKGHADKKNE WWPKMQ++ +L+ESCT 
Sbjct: 661 KTWVANYCSLYYKDDEAIQNDVELQSWWKEAREKGHADKKNEAWWPKMQSINDLVESCTI 720

Query: 721 IIWISSALHAAVNFGQYPYGGFIPNRPTISRRFMPEVGTPEYKELESNPEKAFLRTINSQ 780
           IIWISSALHAAVNFGQYPYGG+IPNRPTISRRFMPEVG+ EYKELES PEKAFLRTINSQ
Sbjct: 721 IIWISSALHAAVNFGQYPYGGYIPNRPTISRRFMPEVGSSEYKELESKPEKAFLRTINSQ 780

Query: 781 LQTLLGVSLIEILSRHASDEVYLGQRASIEWTSDAAAIEVFEKFGKEVFEVENRIIERNK 840
           LQTLLGVSLIEILSRHASDEVYLGQRASIEWTSD AA++ FE FGK+VFEVENRI+ERN+
Sbjct: 781 LQTLLGVSLIEILSRHASDEVYLGQRASIEWTSDKAALDAFENFGKQVFEVENRIMERNR 840

Query: 841 NINFKNRTGPVNVPYTLLLPSSTEGLTGRGIPNSISI 873
           ++N KNR+GPVN+PYTLLLPSSTEGLTGRGIPNSISI
Sbjct: 841 DVNLKNRSGPVNMPYTLLLPSSTEGLTGRGIPNSISI 877

BLAST of MC08g2255 vs. TAIR 10
Match: AT1G55020.1 (lipoxygenase 1 )

HSP 1 Score: 1092.8 bits (2825), Expect = 0.0e+00
Identity = 526/858 (61.31%), Postives = 665/858 (77.51%), Query Frame = 0

Query: 27  GNIVDRVTNLGG----KKIKGKVILMRSNVLDFTEFHSTLLDGFTELLGGGVSLQLISA- 86
           G + D +T  G     KK+KG V+LM+ NVLDF +F+++ LD   E LG  ++L+L+S+ 
Sbjct: 3   GELRDLLTGGGNETTTKKVKGTVVLMKKNVLDFNDFNASFLDRLHEFLGNKITLRLVSSD 62

Query: 87  -TQASLDSRGKVGKRAFLERWLTSIPPLFAGESVFQVNFDLEDNFGLPGAFFIKNGHTSE 146
            T +   S+GK+GK A LE W+T+I  L AGES F+V FD E +FG PGAF I+N H SE
Sbjct: 63  VTDSENGSKGKLGKAAHLEDWITTITSLTAGESAFKVTFDYETDFGYPGAFLIRNSHFSE 122

Query: 147 FFLKSLTLEDVPGFGRIHFDCNSWVYPSGRYKKDRIFFANKTFLPSETPNPLRKYREEEL 206
           F LKSLTLEDVPG GR+H+ CNSW+YP+  Y  DR+FF+NKT+LP ETP  L KYREEEL
Sbjct: 123 FLLKSLTLEDVPGHGRVHYICNSWIYPAKHYTTDRVFFSNKTYLPHETPATLLKYREEEL 182

Query: 207 LNLRGDGTGERKEWDRIYDYDVYNDLSDP--NVRPILGGSQ-HPYPRRGRTGRPRARRDS 266
           ++LRG G GE KEWDR+YDY  YNDL  P  N RP+LGG+Q +PYPRRGRTGR   + D 
Sbjct: 183 VSLRGTGEGELKEWDRVYDYAYYNDLGVPPKNPRPVLGGTQEYPYPRRGRTGRKPTKEDP 242

Query: 267 NYETRLSAVLGLTIYVPRDENFGHLKMSDFLGFALKSLSSTVQPELVNLINITPGEFDKF 326
             E+RL     L IYVPRDE FGHLKMSDFL +ALK+++  +QP L  + + TP EFD F
Sbjct: 243 QTESRLPITSSLDIYVPRDERFGHLKMSDFLAYALKAIAQFIQPALEAVFDDTPKEFDSF 302

Query: 327 QDVHDLYEGGFPVPLN-VFRNLTEAFTPPMFQELLRNDGERFLKFSPPQVVRDNKSGWRT 386
           +DV  +YE G  +P   +  ++ +     M +E+ R DG++FLKF  PQV++++K+ WRT
Sbjct: 303 EDVLKIYEEGIDLPNQALIDSIVKNIPLEMLKEIFRTDGQKFLKFPVPQVIKEDKTAWRT 362

Query: 387 DEEFAREMLAGVNPVIICRLKEFPPLSKLDPQIYGDQNSKITEEDIKFGLEGLSADEAIK 446
           DEEFAREMLAG+NPV+I  LKEFPP SKLD + YG+QNS IT+  I+  L+GL+ +EA++
Sbjct: 363 DEEFAREMLAGLNPVVIQLLKEFPPKSKLDSESYGNQNSTITKSHIEHNLDGLTVEEALE 422

Query: 447 QNKLYILDHHDALMPYLRRINSTSTRTYATRTLLFLKADGTLKPLVIELSLPHPQGDQFG 506
           + +L+ILDHHD LMPYL R+N+T+T+TYA+RTLLFLK DGTLKPLVIELSLPHP GD+FG
Sbjct: 423 KERLFILDHHDTLMPYLGRVNTTTTKTYASRTLLFLKDDGTLKPLVIELSLPHPNGDKFG 482

Query: 507 ANSKLYFPAESGVENSIWQLAKAYVAVNDAGYHQLNSHWLHTHAVQEPFVIATHRQLSVV 566
           A S++Y P E GV +S+WQLAKA+V VND+G HQL SHW+ THA  EPFVIAT+RQLSV+
Sbjct: 483 AVSEVYTPGE-GVYDSLWQLAKAFVGVNDSGNHQLISHWMQTHASIEPFVIATNRQLSVL 542

Query: 567 HPIHKLLVPHFKDTMFINAFARQVLVNADGLLESTHFQSKYAMELSSDVYK-EWNFLEQG 626
           HP+ KLL PHF+DTM INA ARQ+L+N  G+ E T F SKYAME+SS +YK  W F +Q 
Sbjct: 543 HPVFKLLEPHFRDTMNINALARQILINGGGIFEITVFPSKYAMEMSSFIYKNHWTFPDQA 602

Query: 627 LPADLIKRGVAVEDSNSPHGLRLLIEDYPFAVDGLEIWSTIKTWVTDYISVYYKDDKAVQ 686
           LPA+L KRG+AVED  +PHGLRL I+DYP+AVDGLE+W  I++WV DYI ++YK ++ +Q
Sbjct: 603 LPAELKKRGMAVEDPEAPHGLRLRIKDYPYAVDGLEVWYAIESWVRDYIFLFYKIEEDIQ 662

Query: 687 NDVELQSWWKEVREKGHADKKNEPWWPKMQTLKELIESCTTIIWISSALHAAVNFGQYPY 746
            D ELQ+WWKEVRE+GH DKK+EPWWPKMQT +EL+ESCT IIW++SALHAAVNFGQYP 
Sbjct: 663 TDTELQAWWKEVREEGHGDKKSEPWWPKMQTREELVESCTIIIWVASALHAAVNFGQYPV 722

Query: 747 GGFIPNRPTISRRFMPEVGTPEYKELESNPEKAFLRTINSQLQTLLGVSLIEILSRHASD 806
            G++PNRPTISR++MP+  TPE++ELE NP+K FL+TI +QLQTLLG+SLIEILS H+SD
Sbjct: 723 AGYLPNRPTISRQYMPKENTPEFEELEKNPDKVFLKTITAQLQTLLGISLIEILSTHSSD 782

Query: 807 EVYLGQRASIEWTSDAAAIEVFEKFGKEVFEVENRIIERNKNINFKNRTGPVNVPYTLLL 866
           EVYLGQR S EW ++  A+E FEKFG++V E+E  I ERN +   KNRTG V +PYTLL 
Sbjct: 783 EVYLGQRDSKEWAAEKEALEAFEKFGEKVKEIEKNIDERNDDETLKNRTGLVKMPYTLLF 842

Query: 867 PSSTEGLTGRGIPNSISI 874
           PSS  G+TGRGIPNS+SI
Sbjct: 843 PSSEGGVTGRGIPNSVSI 859

BLAST of MC08g2255 vs. TAIR 10
Match: AT3G22400.1 (PLAT/LH2 domain-containing lipoxygenase family protein )

HSP 1 Score: 1064.7 bits (2752), Expect = 4.0e-311
Identity = 522/854 (61.12%), Postives = 654/854 (76.58%), Query Frame = 0

Query: 40  KIKGKVILMRSNVLDFTEFHSTLLDGFTELLGGGVSLQLISATQ--ASLDSRGKVGKRAF 99
           KI+G+V++M+ N+LDF +  ++LLD   ELLG  VSL LIS+ Q   + + RG++GK A 
Sbjct: 34  KIEGEVVVMKKNLLDFKDVMASLLDRVNELLGRRVSLHLISSHQPDPANEKRGRLGKAAH 93

Query: 100 LERWLTSI-PPLFAGESVFQVNFDLEDNFGLPGAFFIKNGHTSEFFLKSLTLEDVP---- 159
           LE+W+T I   + A E+ F V FD +++ G P AF IKN H S+F+LKSLTL   P    
Sbjct: 94  LEKWVTKIKTSVTAEETAFGVTFDWDESMGPPAAFVIKNHHHSQFYLKSLTLRGFPDGEG 153

Query: 160 GFGRIHFDCNSWVYPSGRYKKDRIFFANKTFLPSETPNPLRKYREEELLNLRG-DGTGER 219
           G   IHF CNSW+YP+ RY+ DR+FF+NK +LPSETP  +++ REEEL NLRG +  GE 
Sbjct: 154 GATAIHFICNSWIYPNHRYRSDRVFFSNKAYLPSETPELIKELREEELKNLRGNEKGGEF 213

Query: 220 KEWDRIYDYDVYNDLSDP-----NVRPILGGS-QHPYPRRGRTGRPRARRDSNYETRLSA 279
           KEWDR+YDY  YNDL  P     +VRP+LGGS + PYPRRG+TGR   + D   E+RL A
Sbjct: 214 KEWDRVYDYAYYNDLGAPDKGPDSVRPVLGGSPELPYPRRGKTGRKSTKSDPKSESRL-A 273

Query: 280 VLGLTIYVPRDENFGHLKMSDFLGFALKSLSSTVQPELVNLINITPGEFDKFQDVHDLYE 339
           +L L IYVPRDE F H+K SDFL +ALKS++  + PE+ ++ + T  EFD F+DV  LY+
Sbjct: 274 LLNLNIYVPRDERFSHVKFSDFLAYALKSVTQVLVPEIASVCDKTINEFDSFEDVFHLYD 333

Query: 340 GGFPVPL-NVFRNLTEAFTPPMFQELLRNDGERFLKFSPPQVVRDNKSGWRTDEEFAREM 399
           G   +   +    L +     MF+EL+RNDGERFLK+  P ++++++S WRTDEEFAREM
Sbjct: 334 GSIKLANGHTISKLRDVIPWEMFRELVRNDGERFLKYPLPDILKESRSAWRTDEEFAREM 393

Query: 400 LAGVNPVIICRLKEFPPLSKLDPQIYGDQNSKITEEDIKFGLEGLSADEAIKQNKLYILD 459
           LAG+NPV+I RL+EFPP S LD   YG+Q+S I  E I+  + GL+  EA++QNKLYILD
Sbjct: 394 LAGLNPVVISRLQEFPPKSCLDSAKYGNQHSSIRTEHIESNMNGLNVQEALEQNKLYILD 453

Query: 460 HHDALMPYLRRINSTSTRTYATRTLLFLKADGTLKPLVIELSLPHPQGDQFGANSKLYFP 519
           HHDALMPYL RINST+T+TYATRTLL L+ADGTLKPL IELSLPH QG+ +G+ SK++ P
Sbjct: 454 HHDALMPYLTRINSTNTKTYATRTLLLLQADGTLKPLAIELSLPHAQGESYGSVSKVFTP 513

Query: 520 AESGVENSIWQLAKAYVAVNDAGYHQLNSHWLHTHAVQEPFVIATHRQLSVVHPIHKLLV 579
           AE GVE S+WQLAKAY AVND+GYHQL SHWL THAV EPF+IA++RQLSVVHPIHKLL 
Sbjct: 514 AEKGVEGSVWQLAKAYAAVNDSGYHQLISHWLQTHAVIEPFIIASNRQLSVVHPIHKLLH 573

Query: 580 PHFKDTMFINAFARQVLVNADGLLESTHFQSKYAMELSSDVYKEWNFLEQGLPADLIKRG 639
           PHF+DTM INA AR VL+N+DG+LE T F S+YAME+SS +YK W F EQ LP DL+KRG
Sbjct: 574 PHFRDTMNINALARHVLINSDGVLERTVFPSRYAMEMSSSIYKNWVFTEQALPKDLLKRG 633

Query: 640 VAVEDSNSPHGLRLLIEDYPFAVDGLEIWSTIKTWVTDYISVYYKDDKAVQNDVELQSWW 699
           VAVED NS +G++LLIEDYPFAVDGLEIWS IKTWVT+Y + YY +DK VQ D E+QSWW
Sbjct: 634 VAVEDPNSDNGVKLLIEDYPFAVDGLEIWSAIKTWVTEYCTFYYNNDKTVQTDTEIQSWW 693

Query: 700 KEVREKGHADKKNEPWWPKMQTLKELIESCTTIIWISSALHAAVNFGQYPYGGFIPNRPT 759
            E+R KGH DK++E WWP MQT  +LIE+CT IIWI+SALHAAVNFGQYPY GF+PNRPT
Sbjct: 694 TELRTKGHGDKRHESWWPSMQTRDDLIETCTIIIWIASALHAAVNFGQYPYAGFLPNRPT 753

Query: 760 ISRRFMPEVGTPEYKELESNPEKAFLRTINSQLQTLLGVSLIEILSRHASDEVYLGQRAS 819
           +SRRFMPE GT EY ELE + + AFL+TI  QLQTLLG+S+IEILS H++DE+YLGQR S
Sbjct: 754 VSRRFMPEPGTDEYAELEEDADVAFLKTITPQLQTLLGISIIEILSMHSTDEIYLGQRDS 813

Query: 820 IEWTSDAAAIEVFEKFGKEVFEVENRIIERNKNINFKNRTGPVNVPYTLLLPSSTE---- 874
             WT+D   +E F++FGKE+  +EN II RN +  FKNRTGPVN+PYTLL P++T+    
Sbjct: 814 PNWTADDEPLEAFKRFGKELELIENNIIRRNNDKRFKNRTGPVNIPYTLLYPNTTDYTRE 873

BLAST of MC08g2255 vs. TAIR 10
Match: AT1G72520.1 (PLAT/LH2 domain-containing lipoxygenase family protein )

HSP 1 Score: 718.0 bits (1852), Expect = 9.2e-207
Identity = 375/846 (44.33%), Postives = 524/846 (61.94%), Query Frame = 0

Query: 40  KIKGKVILMRSNVLDFTEFHSTLLDGFTELLGGGVSLQLISATQASLDSRGKVGKRAFLE 99
           K++    +   N  DF E     LD FT+ +G  V L+L+S       +  K  K A L+
Sbjct: 91  KVRAVATVRNKNKEDFKETLVKHLDAFTDKIGRNVVLELMSTQVDPKTNEPKKSKAAVLK 150

Query: 100 RWLTSIPPLFAGESV-FQVNFDLEDNFGLPGAFFIKNGHTSEFFLKSLTLEDVPGF--GR 159
            W  S       E V +   F ++  FG PGA  + N H  EFFL+S+T+E   GF  G 
Sbjct: 151 DW--SKKSNSKAERVHYTAEFTVDSAFGSPGAITVTNKHQKEFFLESITIE---GFACGP 210

Query: 160 IHFDCNSWVYPSGRYKKDRIFFANKTFLPSETPNPLRKYREEELLNLRGDGTGERKEWDR 219
           +HF CNSWV     +   RI F N+ +LPSETP+ LR  RE+EL NLRG+G GERK  DR
Sbjct: 211 VHFPCNSWVQSQKDHPSKRILFTNQPYLPSETPSGLRTLREKELENLRGNGKGERKLSDR 270

Query: 220 IYDYDVYNDLSDPNV-----RPILGGSQHPYPRRGRTGRPRARRDSNYETRLSAVLGLTI 279
           IYDYDVYND+ +P++     RP LGG + PYPRR RTGR     D   E R+     L +
Sbjct: 271 IYDYDVYNDIGNPDISRELARPTLGGREFPYPRRCRTGRSSTDTDMMSERRVEK--PLPM 330

Query: 280 YVPRDENFGHLKMSDFLGFALKSLSSTVQPELVNLINITPGEFDKFQDVHDLYEGGFPVP 339
           YVPRDE F   K + F    LK++   + P L    +I   +F  F ++  LY+ G  + 
Sbjct: 331 YVPRDEQFEESKQNTFAACRLKAVLHNLIPSL--KASILAEDFANFGEIDSLYKEGLLLK 390

Query: 340 LNVFRNLTEAFTPPMFQELLRNDGERFLKFSPPQVVRDNKSGWRTDEEFAREMLAGVNPV 399
           L    ++ + F  P     L+   E  L++  P++V  +K  W  D+EFAR+ +AG+NPV
Sbjct: 391 LGFQDDMFKKFPLPKIVTTLQKSSEGLLRYDTPKIVSKDKYAWLRDDEFARQAIAGINPV 450

Query: 400 IICRLKEFPPLSKLDPQIYG-DQNSKITEEDIKFGLEGLSADEAIKQNKLYILDHHDALM 459
            I R+  +PP+S LDP+IYG   +S +TE+ I   L+GL+  +A++ N+L+++D+HD  +
Sbjct: 451 NIERVTSYPPVSNLDPEIYGPGLHSALTEDHIIGQLDGLTVQQALETNRLFMVDYHDIYL 510

Query: 460 PYLRRINSTSTR-TYATRTLLFLKADGTLKPLVIELSLPHPQGDQFGANSKLYFPAESGV 519
           P+L RIN+   R  YATRT+LFL   GTLKP+ IELSLP  Q      + ++  P     
Sbjct: 511 PFLDRINALDGRKAYATRTILFLTRLGTLKPIAIELSLP-SQSSSNQKSKRVVTPPVDAT 570

Query: 520 ENSIWQLAKAYVAVNDAGYHQLNSHWLHTHAVQEPFVIATHRQLSVVHPIHKLLVPHFKD 579
            N +WQLAKA+V  NDAG HQL +HWL THA  EPF++A HRQLS +HPI KLL PH + 
Sbjct: 571 SNWMWQLAKAHVGSNDAGVHQLVNHWLRTHACLEPFILAAHRQLSAMHPIFKLLDPHMRY 630

Query: 580 TMFINAFARQVLVNADGLLESTHFQSKYAMELSSDVYK-EWNFLEQGLPADLIKRGVAVE 639
           T+ INA ARQ L++ADG++ES     +Y +E+SS  YK +W F  +GLPADLI+RG+AV 
Sbjct: 631 TLEINAVARQTLISADGVIESCFTAGQYGLEISSAAYKNKWRFDMEGLPADLIRRGMAVP 690

Query: 640 DSNSPHGLRLLIEDYPFAVDGLEIWSTIKTWVTDYISVYYKDDKAVQNDVELQSWWKEVR 699
           D   PHGL+LL+EDYP+A DGL +WS I+TWV  Y+  YY +   +Q D ELQ+W+ E  
Sbjct: 691 DPTQPHGLKLLVEDYPYANDGLLLWSAIQTWVRTYVERYYANSNLIQTDTELQAWYSESI 750

Query: 700 EKGHADKKNEPWWPKMQTLKELIESCTTIIWISSALHAAVNFGQYPYGGFIPNRPTISRR 759
             GHAD ++  WWPK+ T+++L+   TTIIW++SA HAA+NFGQYPYGG++PNRP + RR
Sbjct: 751 NVGHADHRDAEWWPKLSTVEDLVSVITTIIWLASAQHAALNFGQYPYGGYVPNRPPLMRR 810

Query: 760 FMPEVGTPEYKELESNPEKAFLRTINSQLQTLLGVSLIEILSRHASDEVYLGQRASIE-W 819
            +P+   PE+     +P+K F  ++ S LQT   +++++ LS H+ DE Y+G+R     W
Sbjct: 811 LIPDESDPEFTSFIEDPQKYFFSSMPSLLQTTKFMAVVDTLSTHSPDEEYIGERQQPSIW 870

Query: 820 TSDAAAIEVFEKFGKEVFEVENRIIERNKNINFKNRTGPVNVPYTLLLPSSTEGLTGRGI 874
           T DA  ++ F  F  E+  +E  I +RN++ + +NR G   +PY L+ PSS  G+T RG+
Sbjct: 871 TGDAEIVDAFYGFSAEIGRIEKEIDKRNRDPSRRNRCGAGVLPYELMAPSSEPGVTCRGV 926

BLAST of MC08g2255 vs. TAIR 10
Match: AT1G17420.1 (lipoxygenase 3 )

HSP 1 Score: 713.8 bits (1841), Expect = 1.7e-205
Identity = 375/846 (44.33%), Postives = 529/846 (62.53%), Query Frame = 0

Query: 40  KIKGKVILMRSNVLDFTEFHSTLLDGFTELLGGGVSLQLISATQASLDSRGKVGKR---A 99
           K++  V +   N  D  E     LD F + +G  + L+LIS     LD + K+ K+   A
Sbjct: 85  KVRAVVTVRNKNKEDLKETLVKHLDAFADKIGRNIVLELIS---TQLDPKTKLPKKSNAA 144

Query: 100 FLERWLTSIPPLFAGESVFQVNFDLEDNFGLPGAFFIKNGHTSEFFLKSLTLEDVPGFGR 159
            L+ W +      A    +   F ++  FG PGA  + N H  EFFL+S+T+E     G 
Sbjct: 145 VLKDW-SKKSKTKAERVHYTAEFTVDAAFGSPGAITVMNKHQKEFFLESITIEGF-ALGP 204

Query: 160 IHFDCNSWVYPSGRYKKDRIFFANKTFLPSETPNPLRKYREEELLNLRGDGTGERKEWDR 219
           +HF CNSWV     +   RIFF N+ +LP+ETP+ LR  RE+EL NLRGDG+G RK  DR
Sbjct: 205 VHFPCNSWVQSQKDHPDKRIFFTNQPYLPNETPSGLRVLREKELKNLRGDGSGVRKLSDR 264

Query: 220 IYDYDVYNDLSDPN-----VRPILGGSQHPYPRRGRTGRPRARRDSNYETRLSAVLGLTI 279
           IYD+DVYNDL +P+      RP LGG + PYPRR RTGR     D + E+R+     L +
Sbjct: 265 IYDFDVYNDLGNPDKSSELSRPKLGGKEVPYPRRCRTGRQSTVSDKDAESRVEK--PLPM 324

Query: 280 YVPRDENFGHLKMSDFLGFALKSLSSTVQPELVNLINITPGEFDKFQDVHDLYEGGFPVP 339
           YVPRDE F   K   F    LK++   + P L    +I   +F  F ++  LY+ G  + 
Sbjct: 325 YVPRDEQFEESKQDTFAAGRLKAVLHHLIPSL--KASIVAEDFADFGEIDRLYKEGLLLK 384

Query: 340 LNVFRNLTEAF-TPPMFQELLRNDGERFLKFSPPQVVRDNKSGWRTDEEFAREMLAGVNP 399
           L    ++ + F  P +  + L+   +  LK+  P+++  +K+ W  D+EFAR+ +AG+NP
Sbjct: 385 LGFQDDIFKKFPLPKVVVDTLQESTKGLLKYDTPKILSKDKNAWLRDDEFARQAIAGINP 444

Query: 400 VIICRLKEFPPLSKLDPQIYGDQNSKITEEDIKFGLEGLSADEAIKQNKLYILDHHDALM 459
           V I R+K FPP+S LDP+IYG Q+S +T++ I   L+G S  +A+++N+LY+LD+HD  +
Sbjct: 445 VNIERVKTFPPVSNLDPKIYGPQHSALTDDHIIGHLDGFSVQQALEENRLYMLDYHDIFL 504

Query: 460 PYLRRINSTSTR-TYATRTLLFLKADGTLKPLVIELSLPHPQGDQFGANSKLYFPAESGV 519
           P+L RIN+   R  YATRT+ FL   GTLKP+ IELSLP P G +   + ++  P     
Sbjct: 505 PFLDRINALDGRKAYATRTIFFLTRLGTLKPVAIELSLP-PHGPKH-RSKRVLTPPVDAT 564

Query: 520 ENSIWQLAKAYVAVNDAGYHQLNSHWLHTHAVQEPFVIATHRQLSVVHPIHKLLVPHFKD 579
            N +WQLAKA+V+ NDAG HQL +HWL THA  EPF++A HRQLS +HPI KLL PH + 
Sbjct: 565 SNWMWQLAKAHVSSNDAGVHQLVNHWLRTHACLEPFILAAHRQLSAMHPIFKLLDPHMRY 624

Query: 580 TMFINAFARQVLVNADGLLESTHFQSKYAMELSSDVYK-EWNFLEQGLPADLIKRGVAVE 639
           T+ INA ARQ L++ADG++E       Y ME+S+  YK  W F  +GLPADLI+RG+A+ 
Sbjct: 625 TLEINALARQSLISADGVIEGGFTAGAYGMEMSAAAYKSSWRFDMEGLPADLIRRGMAIP 684

Query: 640 DSNSPHGLRLLIEDYPFAVDGLEIWSTIKTWVTDYISVYYKDDKAVQNDVELQSWWKEVR 699
           D+  PHGL+LLIEDYP+A DGL +WS I+TWV  Y+  YY +   ++ D ELQSW+ E  
Sbjct: 685 DATQPHGLKLLIEDYPYANDGLLLWSAIQTWVRTYVERYYPNPNLIKTDSELQSWYSESI 744

Query: 700 EKGHADKKNEPWWPKMQTLKELIESCTTIIWISSALHAAVNFGQYPYGGFIPNRPTISRR 759
             GHAD ++  WWP++ T+ +L+   TT+IW++SA HAA+NFGQYPYGG++PNRP + RR
Sbjct: 745 NVGHADLRDADWWPELSTVDDLVSILTTLIWLASAQHAALNFGQYPYGGYVPNRPPLMRR 804

Query: 760 FMPEVGTPEYKELESNPEKAFLRTINSQLQTLLGVSLIEILSRHASDEVYLGQRASIE-W 819
            +P+   PEY    S+PEK +  ++ S  QT   +++++ LS H+ DE Y+G+R     W
Sbjct: 805 LIPDESDPEYASFISHPEKYYFSSMPSLAQTSKFMAVVDTLSTHSPDEEYIGERQQPSIW 864

Query: 820 TSDAAAIEVFEKFGKEVFEVENRIIERNKNINFKNRTGPVNVPYTLLLPSSTEGLTGRGI 874
           T DA  +E F  F  E+  +E  I +RN + + +NR G   +PY LL+PSS  G+T RG+
Sbjct: 865 TGDAEIVEAFYGFAAEIGRIEKEIEKRNADPDRRNRCGAGVLPYELLVPSSEPGVTCRGV 919

BLAST of MC08g2255 vs. TAIR 10
Match: AT1G67560.1 (PLAT/LH2 domain-containing lipoxygenase family protein )

HSP 1 Score: 669.1 bits (1725), Expect = 4.9e-192
Identity = 362/857 (42.24%), Postives = 521/857 (60.79%), Query Frame = 0

Query: 38  GKKIKGKVILMRSNVLD-FTEFHSTLLDGFTELLGGGVSLQLISATQASLDSRGKVGKRA 97
           G K    V+ +R  + +  TE     L+ F + +G G+ +QL+S     +D     G+++
Sbjct: 78  GSKTVTAVVKIRKKIKEKLTERFEHQLELFMKAIGQGMLIQLVS---EEIDPETGKGRKS 137

Query: 98  FLERWLTSIPPLFAGES--VFQVNFDLEDNFGLPGAFFIKNGHTSEFFLKSLTLEDVPGF 157
            LE  +  +P         VF  +F +  NFG PGA  + N  ++E  L  + +ED    
Sbjct: 138 -LESPVMGLPKAVKDPRYLVFTADFTVPINFGKPGAILVTNLLSTEICLSEIIIED--ST 197

Query: 158 GRIHFDCNSWVYPSGRYKKDRIFFANKTFLPSETPNPLRKYREEELLNLRGDGTGERKEW 217
             I F  N+W++      + RI F ++  LPSETP+ +++ RE++L+++RGDG GERK  
Sbjct: 198 DTILFPANTWIHSKNDNPQARIIFRSQPCLPSETPDGIKELREKDLVSVRGDGKGERKPH 257

Query: 218 DRIYDYDVYNDLSDP----NVRPILGGSQHPYPRRGRTGRPRARRDSNYETRLSAVLGLT 277
           +RIYDYDVYNDL DP     VRP+LG  + PYPRR RTGRP   +D   E+R        
Sbjct: 258 ERIYDYDVYNDLGDPRKTERVRPVLGVPETPYPRRCRTGRPLVSKDPPCESR--GKEKEE 317

Query: 278 IYVPRDENFGHLKMSDFLGFALKSLSSTVQPELVNLINITPGEFDKFQDVHDLYEGGFPV 337
            YVPRDE F  +K   F     K+L   + P +   ++     F  F D+ +LY+     
Sbjct: 318 FYVPRDEVFEEIKRDTFRAGRFKALFHNLVPSIAAALSNLDIPFTCFSDIDNLYKS---- 377

Query: 338 PLNVFRNLTEAFTPPM------FQELLRNDGERFLKFSPPQVVRDNKSGWRTDEEFAREM 397
             N+    TE     +      F   + N  E  LK+  P V++ ++  W  D EF R+ 
Sbjct: 378 --NIVLGHTEPKDTGLGGFIGGFMNGILNVTETLLKYDTPAVIKWDRFAWLRDNEFGRQA 437

Query: 398 LAGVNPVIICRLKEFPPLSKLDPQIYGDQNSKITEEDIKFGLE--GLSADEAIKQNKLYI 457
           LAGVNPV I  LKE P  S LDP +YG Q S +TEE I   +E  G + ++A+++ +L++
Sbjct: 438 LAGVNPVNIELLKELPIRSNLDPALYGPQESVLTEEIIAREVEHYGTTIEKALEEKRLFL 497

Query: 458 LDHHDALMPYLRRINSTS---TRTYATRTLLFLKADGTLKPLVIELSLPHPQGDQFGANS 517
           +D+HD L+P++ +INS      +TYA+RT+ F   +G L+PL IELSLP P  +    N 
Sbjct: 498 VDYHDILLPFVEKINSIKEDPRKTYASRTIFFYSKNGALRPLAIELSLP-PTAE--SENK 557

Query: 518 KLYFPAESGVENSIWQLAKAYVAVNDAGYHQLNSHWLHTHAVQEPFVIATHRQLSVVHPI 577
            +Y        + IW+LAKA+V  NDAG HQL +HWL THA  EP++IAT+RQLS +HP+
Sbjct: 558 FVYTHGHDATTHWIWKLAKAHVCSNDAGVHQLVNHWLRTHASMEPYIIATNRQLSTMHPV 617

Query: 578 HKLLVPHFKDTMFINAFARQVLVNADGLLESTHFQSKYAMELSSDVYKE-WNFLEQGLPA 637
           +KLL PH + T+ INA AR+ L+N  G++ES     KYAMELSS  YK  W F  +GLPA
Sbjct: 618 YKLLHPHMRYTLEINARARKSLINGGGIIESCFTPGKYAMELSSAAYKSMWRFDMEGLPA 677

Query: 638 DLIKRGVAVEDSNSPHGLRLLIEDYPFAVDGLEIWSTIKTWVTDYISVYYKDDKAVQNDV 697
           DL++RG+A EDS++  G+RL+I+DYP+A DGL IW  IK  V  Y+  +Y D K++ +D+
Sbjct: 678 DLVRRGMAEEDSSAECGVRLVIDDYPYAADGLLIWKAIKDLVESYVKHFYSDSKSITSDL 737

Query: 698 ELQSWWKEVREKGHADKKNEPWWPKMQTLKELIESCTTIIWISSALHAAVNFGQYPYGGF 757
           ELQ+WW E++ KGH DKK+EPWWPK+ T ++L +  T +IWI+S  HAA+NFGQYP+GG+
Sbjct: 738 ELQAWWDEIKNKGHYDKKDEPWWPKLNTTQDLSQILTNMIWIASGQHAAINFGQYPFGGY 797

Query: 758 IPNRPTISRRFMPEVGTPEYKELESNPEKAFLRTINSQLQTLLGVSLIEILSRHASDEVY 817
           +PNRPT+ R+ +P+   P+Y+    NP+ +FL ++ +QLQ    +++ E LS H+ DE Y
Sbjct: 798 VPNRPTLLRKLIPQETDPDYEMFMRNPQYSFLGSLPTQLQATKVMAVQETLSTHSPDEEY 857

Query: 818 LGQRASIE--WTSDAAAIEVFEKFGKEVFEVENRIIERNKNINFKNRTGPVNVPYTLLLP 874
           L +   ++  W  D   ++ F KF +E+ ++E  I ERNK+   KNRTG    PY LLLP
Sbjct: 858 LIELREVQRHWFQDEQVVKYFNKFSEELVKIEKTINERNKDKKLKNRTGAGMPPYELLLP 917