MC08g2118 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g2118 vs. ExPASy Swiss-Prot
Match: M4ISH0 (4-coumarate--CoA ligase CCL1 OS=Humulus lupulus OX=3486 GN=CCL1 PE=1 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 6.8e-229
Identity = 389/533 (72.98%), Postives = 461/533 (86.49%), Query Frame = 0

Query: 9   DDFIFRSKLPDIYIPDHLPLHTYCFDRLSQFQHRPCLINAATGHVHTYAAVHLTARRVAA 68
           ++FIFRSKLPDIYIP+HLPLH+YCF+ +SQF+ RPCLIN ATG + TYA V LT+R+VAA
Sbjct: 11  EEFIFRSKLPDIYIPNHLPLHSYCFENISQFKDRPCLINGATGEIITYADVDLTSRKVAA 70

Query: 69  GLANIGISQGDVIMILLQNSPEFVFAFLGASYGGAISTTANPLYKPAEIAKQARAANAKV 128
           GL  +GI QGDVIM+LLQNSPEFV+AFL ASY GAI TTANP Y PAE+AKQA A+  K+
Sbjct: 71  GLDKLGIKQGDVIMLLLQNSPEFVYAFLAASYIGAIITTANPFYTPAEVAKQAAASKTKL 130

Query: 129 IITQSAFVDKIKGF-AEERGVKILCTDSPP--DGCLSFSELTEADENDIPAVKINSNDVV 188
           +IT + ++DK+K F   E GVK++C D+PP    CL FSELT+ADE +IPAVKI+ +DVV
Sbjct: 131 VITLAGYIDKVKEFTGGESGVKVMCVDAPPPESECLHFSELTQADETEIPAVKIHPDDVV 190

Query: 189 ALPYSSGTTGIPKGVMLTHKSLVTSVAQQVDGENPNLYFKSKDVILCILPLFHIYSLNSV 248
           ALPYSSGTTG+PKGVMLTHK LVTSVAQQVDG+NPNLYF   DVILC+LPLFHIYSLNS+
Sbjct: 191 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFHQNDVILCVLPLFHIYSLNSI 250

Query: 249 LLCGLRVGAAILIIQKYDMSSLLHLIQAHKATVAPFVPPIVLDLAKNPDIHRYDLSSIRI 308
           LLCGLRVGAAILI+QK+++S LL LI+  K T+APFVPPIVL +AK PD+HRYDLSSIR 
Sbjct: 251 LLCGLRVGAAILIMQKFEISKLLELIEKFKVTIAPFVPPIVLSVAKCPDLHRYDLSSIRT 310

Query: 309 VMSGAAPLGKDLEDTLKARLPNANLGQGYGMTEAGPVLSMSLTFAKEPMAVKSGACGTVV 368
           VMSG AP+GK+LED +K +LP+A LGQGYGMTEAGPVLSM L FAKEP  +KSGACGTVV
Sbjct: 311 VMSGGAPMGKELEDAVKEKLPHAKLGQGYGMTEAGPVLSMCLAFAKEPFPIKSGACGTVV 370

Query: 369 RNAEMKIIHPQTGASLPRNQPGEICIRGSQIMKGKYLNNKEATEETIDKDGWLHTGDLGF 428
           RNAEMKI+ P TGASLPRNQ GEICIRG QIMKG Y+N+ EAT+ TID+ GWLHTGD+GF
Sbjct: 371 RNAEMKIVDPDTGASLPRNQSGEICIRGKQIMKG-YINDAEATKGTIDEGGWLHTGDIGF 430

Query: 429 IDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNIAAAAVIPMKDEAAGEIPVAFV 488
           ID+DDE+FIVDRLKELIKYKG+QVAPAELE++L S+PNI  AAV+PMKDEAAGE+PVAFV
Sbjct: 431 IDNDDELFIVDRLKELIKYKGFQVAPAELESMLISHPNITDAAVVPMKDEAAGEVPVAFV 490

Query: 489 VRSGGSKISEDEIKEYVSEQVIFYKKIRKVFLVDSIPIAPSGKILRKVLRDQL 539
           VRS GSKI+E++IK+Y+S+QV+FYK+I K F ++ IP  PSGKILRK+LR +L
Sbjct: 491 VRSNGSKITEEDIKQYISKQVVFYKRINKAFFIEEIPKNPSGKILRKILRAKL 542

BLAST of MC08g2118 vs. ExPASy Swiss-Prot
Match: I3PB37 (4-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1)

HSP 1 Score: 788.9 bits (2036), Expect = 3.7e-227
Identity = 382/532 (71.80%), Postives = 457/532 (85.90%), Query Frame = 0

Query: 10  DFIFRSKLPDIYIPDHLPLHTYCFDRLSQFQHRPCLINAATGHVHTYAAVHLTARRVAAG 69
           D IFRSKLPDIYIP HLPLH+YCF+ +S+F  RPCLIN A  H++TYA V LT+R+VAAG
Sbjct: 11  DLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANNHIYTYADVELTSRKVAAG 70

Query: 70  LANIGISQGDVIMILLQNSPEFVFAFLGASYGGAISTTANPLYKPAEIAKQARAANAKVI 129
           L  +GI Q D IMILL NSPEFVFAF+GASY GAIST ANPL+ PAE+ KQA+A+NAK+I
Sbjct: 71  LNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKASNAKLI 130

Query: 130 ITQSAFVDKIKGFAEERGVKILCTDSPPDGCLSFSELTEADENDIPAVKINSNDVVALPY 189
           ITQ+ FV+K+K +A +  + ++C DS P+GC+ FSELT+ADE+DIP VKI S+DVVALPY
Sbjct: 131 ITQACFVNKVKDYAFDNNLNVICIDSAPEGCIHFSELTQADEHDIPDVKIQSDDVVALPY 190

Query: 190 SSGTTGIPKGVMLTHKSLVTSVAQQVDGENPNLYFKSKDVILCILPLFHIYSLNSVLLCG 249
           SSGTTG+PKGVMLTHK LVTSVAQQVDGEN NLY  S+DV++C+LPLFHIYSLNSVLLCG
Sbjct: 191 SSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLNSVLLCG 250

Query: 250 LRVGAAILIIQKYDMSSLLHLIQAHKATVAPFVPPIVLDLAKNPDIHRYDLSSIRIVMSG 309
           LRVGAAILI+QK+D+     LI+ +K T+ PFVPPIVL +AK+P +  YDLSS+R VMSG
Sbjct: 251 LRVGAAILIMQKFDIVQFCELIEKYKVTIGPFVPPIVLAIAKSPVVDNYDLSSVRTVMSG 310

Query: 310 AAPLGKDLEDTLKARLPNANLGQGYGMTEAGPVLSMSLTFAKEPMAVKSGACGTVVRNAE 369
           AAPLGK+LED ++ + PNA LGQGYGMTEAGPVL+M L FAKEP  +KSGACGTVVRNAE
Sbjct: 311 AAPLGKELEDAVRIKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVRNAE 370

Query: 370 MKIIHPQTGASLPRNQPGEICIRGSQIMKGKYLNNKEATEETIDKDGWLHTGDLGFIDDD 429
           MKI+ P TG SLPRNQPGEICIRG QIMKG YLN+  AT  TIDK+GWLHTGD+G+ID+D
Sbjct: 371 MKIVDPDTGCSLPRNQPGEICIRGDQIMKG-YLNDPAATTRTIDKEGWLHTGDIGYIDND 430

Query: 430 DEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNIAAAAVIPMKDEAAGEIPVAFVVRSG 489
           DE+FIVDRLKELIKYKG+QVAPAELEALL ++PNI+ AAV+PMKDE AGE+PVAFVVRS 
Sbjct: 431 DELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSN 490

Query: 490 GSKISEDEIKEYVSEQVIFYKKIRKVFLVDSIPIAPSGKILRKVLRDQLQAG 542
           GS I+EDE+K++VS+QVIFYK+I++VF V+++P +PSGKILRK LR +L AG
Sbjct: 491 GSDITEDEVKDFVSKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 541

BLAST of MC08g2118 vs. ExPASy Swiss-Prot
Match: O24145 (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 788.9 bits (2036), Expect = 3.7e-227
Identity = 380/532 (71.43%), Postives = 456/532 (85.71%), Query Frame = 0

Query: 10  DFIFRSKLPDIYIPDHLPLHTYCFDRLSQFQHRPCLINAATGHVHTYAAVHLTARRVAAG 69
           D IFRSKLPDIYIP HLPLH+YCF+ +S+F  RPCLIN A   ++TYA V LT R+VA G
Sbjct: 14  DLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTCRKVAVG 73

Query: 70  LANIGISQGDVIMILLQNSPEFVFAFLGASYGGAISTTANPLYKPAEIAKQARAANAKVI 129
           L  +GI Q D IMILL NSPEFVFAF+GASY GAIST ANPL+ PAE+ KQA+A++AK+I
Sbjct: 74  LNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKASSAKII 133

Query: 130 ITQSAFVDKIKGFAEERGVKILCTDSPPDGCLSFSELTEADENDIPAVKINSNDVVALPY 189
           ITQS FV K+K +A E  VK++C DS P+GCL FSELT++DE++IP VKI  +DVVALPY
Sbjct: 134 ITQSCFVGKVKDYASENDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQPDDVVALPY 193

Query: 190 SSGTTGIPKGVMLTHKSLVTSVAQQVDGENPNLYFKSKDVILCILPLFHIYSLNSVLLCG 249
           SSGTTG+PKGVMLTHK LVTSVAQQVDGEN NLY  S+DV++C+LPLFHIYSLNS+LLCG
Sbjct: 194 SSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLNSILLCG 253

Query: 250 LRVGAAILIIQKYDMSSLLHLIQAHKATVAPFVPPIVLDLAKNPDIHRYDLSSIRIVMSG 309
           LRVGAAILI+QK+D++  L LIQ +K ++ PFVPPIVL +AK+P +  YDLSS+R VMSG
Sbjct: 254 LRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSSVRTVMSG 313

Query: 310 AAPLGKDLEDTLKARLPNANLGQGYGMTEAGPVLSMSLTFAKEPMAVKSGACGTVVRNAE 369
           AAPLGK+LED ++ + PNA LGQGYGMTEAGPVL+M L FAKEP  +KSGACGTVVRNAE
Sbjct: 314 AAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVRNAE 373

Query: 370 MKIIHPQTGASLPRNQPGEICIRGSQIMKGKYLNNKEATEETIDKDGWLHTGDLGFIDDD 429
           MKI+ P TG SLPRNQPGEICIRG QIMKG YLN+ EAT  TIDK+GWLHTGD+GFID+D
Sbjct: 374 MKIVDPDTGCSLPRNQPGEICIRGDQIMKG-YLNDPEATTRTIDKEGWLHTGDIGFIDED 433

Query: 430 DEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNIAAAAVIPMKDEAAGEIPVAFVVRSG 489
           DE+FIVDRLKELIKYKG+QVAPAE+EALL ++PNI+ AAV+PMKDE AGE+PVAFVVRS 
Sbjct: 434 DELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSN 493

Query: 490 GSKISEDEIKEYVSEQVIFYKKIRKVFLVDSIPIAPSGKILRKVLRDQLQAG 542
           GS I+EDE+K+++S+QVIFYK++++VF V+++P +PSGKILRK LR +L AG
Sbjct: 494 GSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAG 544

BLAST of MC08g2118 vs. ExPASy Swiss-Prot
Match: O24146 (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)

HSP 1 Score: 784.3 bits (2024), Expect = 9.1e-226
Identity = 385/532 (72.37%), Postives = 458/532 (86.09%), Query Frame = 0

Query: 10  DFIFRSKLPDIYIPDHLPLHTYCFDRLSQFQHRPCLINAATGHVHTYAAVHLTARRVAAG 69
           D IFRSKLPDIYIP+HLPLH+YCF+ +S+F  RPCLIN A   ++TYA V L +R+VAAG
Sbjct: 9   DIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELNSRKVAAG 68

Query: 70  LANIGISQGDVIMILLQNSPEFVFAFLGASYGGAISTTANPLYKPAEIAKQARAANAKVI 129
           L   GI   D IMILL NSPEFVFAF+GASY GAIST ANPL+ PAE+ KQA+A++AK+I
Sbjct: 69  LHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKII 128

Query: 130 ITQSAFVDKIKGFAEERGVKILCTDSPPDGCLSFSELTEADENDIPAVKINSNDVVALPY 189
           +TQ+  V+K+K +A E  VKI+C DS P+GCL FS LT+A+E+DIP V+I  +DVVALPY
Sbjct: 129 VTQACHVNKVKDYAFENDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEIQPDDVVALPY 188

Query: 190 SSGTTGIPKGVMLTHKSLVTSVAQQVDGENPNLYFKSKDVILCILPLFHIYSLNSVLLCG 249
           SSGTTG+PKGVMLTHK LVTSVAQQVDGENPNLY  S+DV+LC+LPLFHIYSLNSVLLCG
Sbjct: 189 SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIYSLNSVLLCG 248

Query: 250 LRVGAAILIIQKYDMSSLLHLIQAHKATVAPFVPPIVLDLAKNPDIHRYDLSSIRIVMSG 309
           LRVGAAILI+QK+D+ S L LIQ +K T+ PFVPPIVL +AK+P +  YDLSS+R VMSG
Sbjct: 249 LRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDLSSVRTVMSG 308

Query: 310 AAPLGKDLEDTLKARLPNANLGQGYGMTEAGPVLSMSLTFAKEPMAVKSGACGTVVRNAE 369
           AAPLGK+LEDT++A+ PNA LGQGYGMTEAGPVL+M L FAKEP  +KSGACGTVVRNAE
Sbjct: 309 AAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVRNAE 368

Query: 370 MKIIHPQTGASLPRNQPGEICIRGSQIMKGKYLNNKEATEETIDKDGWLHTGDLGFIDDD 429
           MKI+ P+TG SLPRNQ GEICIRG QIMKG YLN+ EAT  TIDK+GWL+TGD+G+IDDD
Sbjct: 369 MKIVDPKTGNSLPRNQSGEICIRGDQIMKG-YLNDPEATARTIDKEGWLYTGDIGYIDDD 428

Query: 430 DEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNIAAAAVIPMKDEAAGEIPVAFVVRSG 489
           DE+FIVDRLKELIKYKG+QVAPAELEALL ++PNI+ AAV+PMKDE AGE+PVAFVVRS 
Sbjct: 429 DELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSN 488

Query: 490 GSKISEDEIKEYVSEQVIFYKKIRKVFLVDSIPIAPSGKILRKVLRDQLQAG 542
           GS I+EDE+K+++S+QVIFYK+I++VF VD+IP +PSGKILRK LR +L AG
Sbjct: 489 GSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAG 539

BLAST of MC08g2118 vs. ExPASy Swiss-Prot
Match: P31685 (4-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1)

HSP 1 Score: 781.2 bits (2016), Expect = 7.7e-225
Identity = 378/541 (69.87%), Postives = 455/541 (84.10%), Query Frame = 0

Query: 1   MDSQIPTGDDFIFRSKLPDIYIPDHLPLHTYCFDRLSQFQHRPCLINAATGHVHTYAAVH 60
           MD +     D IFRSKLPDIYIP HLPLH+YCF+ LS+F  RPCLI+ A   ++TYA V 
Sbjct: 3   MDIETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVE 62

Query: 61  LTARRVAAGLANIGISQGDVIMILLQNSPEFVFAFLGASYGGAISTTANPLYKPAEIAKQ 120
           LT+R+VA GL  +GI Q D IMILL N PEFVFAF+GASY GAIST ANPL+ PAE+ KQ
Sbjct: 63  LTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 ARAANAKVIITQSAFVDKIKGFAEERGVKILCTDSPPDGCLSFSELTEADENDIPAVKIN 180
           A+A++AK++ITQ+ F  K+K +A E  +K++C DS P+GC+ FSEL ++DE++IP VKI 
Sbjct: 123 AKASSAKIVITQACFAGKVKDYAIENDLKVICVDSAPEGCVHFSELIQSDEHEIPDVKIQ 182

Query: 181 SNDVVALPYSSGTTGIPKGVMLTHKSLVTSVAQQVDGENPNLYFKSKDVILCILPLFHIY 240
            +DVVALPYSSGTTG+PKGVMLTHK LVTSVAQQVDGEN NLY  S DV++C+LPLFHIY
Sbjct: 183 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIY 242

Query: 241 SLNSVLLCGLRVGAAILIIQKYDMSSLLHLIQAHKATVAPFVPPIVLDLAKNPDIHRYDL 300
           SLNSVLLC LRVGAAILI+QK+D++  L LI  HK T+ PFVPPIVL +AK+P +H YDL
Sbjct: 243 SLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVHNYDL 302

Query: 301 SSIRIVMSGAAPLGKDLEDTLKARLPNANLGQGYGMTEAGPVLSMSLTFAKEPMAVKSGA 360
           SS+R VMSGAAPLGK+LED ++A+ PNA LGQGYGMTEAGPVL+M L FAKEP  +KSGA
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIIHPQTGASLPRNQPGEICIRGSQIMKGKYLNNKEATEETIDKDGWLHT 420
           CGTVVRNAEMKI+ P TG SLPRNQPGEICIRG QIMKG YLN+ EAT  TI+K+GWLHT
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKG-YLNDPEATARTIEKEGWLHT 422

Query: 421 GDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNIAAAAVIPMKDEAAGEI 480
           GD+GFIDDDDE+FIVDRLKELIKYKG+QVAPAELEALL ++P+I+ AAV+PM DE AGE+
Sbjct: 423 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEV 482

Query: 481 PVAFVVRSGGSKISEDEIKEYVSEQVIFYKKIRKVFLVDSIPIAPSGKILRKVLRDQLQA 540
           PVAFVVRS GS I+EDE+K+++S+QVIFYK+I++VF V+++P +PSGKILRK LR +L A
Sbjct: 483 PVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAA 542

Query: 541 G 542
           G
Sbjct: 543 G 542

BLAST of MC08g2118 vs. NCBI nr
Match: XP_022153289.1 (4-coumarate--CoA ligase 1-like [Momordica charantia])

HSP 1 Score: 1063 bits (2748), Expect = 0.0
Identity = 542/543 (99.82%), Postives = 542/543 (99.82%), Query Frame = 0

Query: 1   MDSQIPTGDDFIFRSKLPDIYIPDHLPLHTYCFDRLSQFQHRPCLINAATGHVHTYAAVH 60
           MDSQIPTGDDFIFRSKLPDIYIPDHLPLHTYCFDRLSQFQHRPCLINAATGHVHTYAAVH
Sbjct: 1   MDSQIPTGDDFIFRSKLPDIYIPDHLPLHTYCFDRLSQFQHRPCLINAATGHVHTYAAVH 60

Query: 61  LTARRVAAGLANIGISQGDVIMILLQNSPEFVFAFLGASYGGAISTTANPLYKPAEIAKQ 120
           LTARRVAAGLANIGISQGDVIMILLQNSPEFVFAFLGASYGGAISTTANPLYKPAEIAKQ
Sbjct: 61  LTARRVAAGLANIGISQGDVIMILLQNSPEFVFAFLGASYGGAISTTANPLYKPAEIAKQ 120

Query: 121 ARAANAKVIITQSAFVDKIKGFAEERGVKILCTDSPPDGCLSFSELTEADENDIPAVKIN 180
           ARAANAKVIITQSAFVDKIKGFAEERGVKILCTDSPPDGCLSFSELTEADENDIPAVKIN
Sbjct: 121 ARAANAKVIITQSAFVDKIKGFAEERGVKILCTDSPPDGCLSFSELTEADENDIPAVKIN 180

Query: 181 SNDVVALPYSSGTTGIPKGVMLTHKSLVTSVAQQVDGENPNLYFKSKDVILCILPLFHIY 240
           SNDVVALPYSSGTTGIPKGVMLTHKSLVTSVAQQVDGENPNLYFKSKDVILCILPLFHIY
Sbjct: 181 SNDVVALPYSSGTTGIPKGVMLTHKSLVTSVAQQVDGENPNLYFKSKDVILCILPLFHIY 240

Query: 241 SLNSVLLCGLRVGAAILIIQKYDMSSLLHLIQAHKATVAPFVPPIVLDLAKNPDIHRYDL 300
           SLNSVLLCGLRVGAAILIIQKYDMSSLLHLIQAHKATVAPFVPPIVLDLAKNPDIHRYDL
Sbjct: 241 SLNSVLLCGLRVGAAILIIQKYDMSSLLHLIQAHKATVAPFVPPIVLDLAKNPDIHRYDL 300

Query: 301 SSIRIVMSGAAPLGKDLEDTLKARLPNANLGQGYGMTEAGPVLSMSLTFAKEPMAVKSGA 360
           SSIRIVMSGAAPLGKDLEDTLKARLPNANLGQGYGMTEAGPVLSMSLTFAKEPMAVKSGA
Sbjct: 301 SSIRIVMSGAAPLGKDLEDTLKARLPNANLGQGYGMTEAGPVLSMSLTFAKEPMAVKSGA 360

Query: 361 CGTVVRNAEMKIIHPQTGASLPRNQPGEICIRGSQIMKGKYLNNKEATEETIDKDGWLHT 420
           CGTVVRNAEMKIIHPQTGASLPRNQPGEICIRGSQIMKG YLNNKEATEETIDKDGWLHT
Sbjct: 361 CGTVVRNAEMKIIHPQTGASLPRNQPGEICIRGSQIMKG-YLNNKEATEETIDKDGWLHT 420

Query: 421 GDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNIAAAAVIPMKDEAAGEI 480
           GDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNIAAAAVIPMKDEAAGEI
Sbjct: 421 GDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNIAAAAVIPMKDEAAGEI 480

Query: 481 PVAFVVRSGGSKISEDEIKEYVSEQVIFYKKIRKVFLVDSIPIAPSGKILRKVLRDQLQA 540
           PVAFVVRSGGSKISEDEIKEYVSEQVIFYKKIRKVFLVDSIPIAPSGKILRKVLRDQLQA
Sbjct: 481 PVAFVVRSGGSKISEDEIKEYVSEQVIFYKKIRKVFLVDSIPIAPSGKILRKVLRDQLQA 540

Query: 541 GAF 543
           GAF
Sbjct: 541 GAF 542

BLAST of MC08g2118 vs. NCBI nr
Match: XP_022929776.1 (4-coumarate--CoA ligase 2-like [Cucurbita moschata])

HSP 1 Score: 918 bits (2373), Expect = 0.0
Identity = 455/543 (83.79%), Postives = 496/543 (91.34%), Query Frame = 0

Query: 1   MDSQIPTGDDFIFRSKLPDIYIPDHLPLHTYCFDRLSQFQHRPCLINAATGHVHTYAAVH 60
           MDSQ+    DFIFRSKLPDIYIP+HLPLHTYCF+ LSQF HRPCLINAATG +HTYAAV 
Sbjct: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60

Query: 61  LTARRVAAGLANIGISQGDVIMILLQNSPEFVFAFLGASYGGAISTTANPLYKPAEIAKQ 120
           L ARRVA GL+ IG+ QGDVIM+LLQNSPEFVFAFLGASY GAISTTANPLYKPAEI KQ
Sbjct: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120

Query: 121 ARAANAKVIITQSAFVDKIKGFAEERGVKILCTDSPPDGCLSFSELTEADENDIPAVKIN 180
           A  A  KVIITQ+ FV+K++ FA E GVKI+CTDSPP GCL FSE+ EADEN+IPAVKIN
Sbjct: 121 AATARPKVIITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180

Query: 181 SNDVVALPYSSGTTGIPKGVMLTHKSLVTSVAQQVDGENPNLYFKSKDVILCILPLFHIY 240
           SNDVVALP+SSGTTG+PKGVMLTHKSLVTS+AQQVDGENPN+   S DV++C+LPLFHIY
Sbjct: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240

Query: 241 SLNSVLLCGLRVGAAILIIQKYDMSSLLHLIQAHKATVAPFVPPIVLDLAKNPDIHRYDL 300
           SLNSVLLCGLRVGAAILI+QKYDMSSLL LIQ HKAT+APFVPPIVLD AKNPDIHRYDL
Sbjct: 241 SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300

Query: 301 SSIRIVMSGAAPLGKDLEDTLKARLPNANLGQGYGMTEAGPVLSMSLTFAKEPMAVKSGA 360
           SSIRIVMSGAAP+GK LEDT+KARLPNA LGQGYGMTEAGPVLSM L FAKEP   KSGA
Sbjct: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360

Query: 361 CGTVVRNAEMKIIHPQTGASLPRNQPGEICIRGSQIMKGKYLNNKEATEETIDKDGWLHT 420
           CGTVVRNAEMKIIHP+TG SLPRN PGEICIRGSQIMKG YLNNKEATE+TIDK+GWLHT
Sbjct: 361 CGTVVRNAEMKIIHPETGVSLPRNHPGEICIRGSQIMKG-YLNNKEATEKTIDKNGWLHT 420

Query: 421 GDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNIAAAAVIPMKDEAAGEI 480
           GDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPN+++AAV+PM+DEAAGEI
Sbjct: 421 GDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEI 480

Query: 481 PVAFVVRSGGSKISEDEIKEYVSEQVIFYKKIRKVFLVDSIPIAPSGKILRKVLRDQLQA 540
           P AFVVRS GSKISED+IK+Y+S+QVI+YKKIRKVF VDSIP+APSGKILRKVL+ QL+ 
Sbjct: 481 PAAFVVRSKGSKISEDDIKKYISDQVIYYKKIRKVFFVDSIPVAPSGKILRKVLKAQLEN 540

Query: 541 GAF 543
           GAF
Sbjct: 541 GAF 542

BLAST of MC08g2118 vs. NCBI nr
Match: XP_038890953.1 (4-coumarate--CoA ligase 2-like [Benincasa hispida])

HSP 1 Score: 917 bits (2371), Expect = 0.0
Identity = 458/543 (84.35%), Postives = 499/543 (91.90%), Query Frame = 0

Query: 1   MDSQIPTGDDFIFRSKLPDIYIPDHLPLHTYCFDRLSQFQHRPCLINAATGHVHTYAAVH 60
           MD  +  G DFIFRSKLPDIYIP+HLPLHTYCF+ LSQFQHRPCLIN ATG VHTYAAV 
Sbjct: 1   MDHGLMNGQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGATGEVHTYAAVD 60

Query: 61  LTARRVAAGLANIGISQGDVIMILLQNSPEFVFAFLGASYGGAISTTANPLYKPAEIAKQ 120
           L ARRVAAGL+ IG+ QGDVIM++LQNSPEFVFAFLGAS+ GAISTTANPLYKP EIAKQ
Sbjct: 61  LAARRVAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAISTTANPLYKPGEIAKQ 120

Query: 121 ARAANAKVIITQSAFVDKIKGFAEERGVKILCTDSPPDGCLSFSELTEADENDIPAVKIN 180
           A  A  KVIITQ+ FV+K+ G+A E GVKILCTDSPP GCL FSE+ EADENDIPAVKIN
Sbjct: 121 AAGARPKVIITQAEFVEKVWGYAVEHGVKILCTDSPPVGCLRFSEVMEADENDIPAVKIN 180

Query: 181 SNDVVALPYSSGTTGIPKGVMLTHKSLVTSVAQQVDGENPNLYFKSKDVILCILPLFHIY 240
           SNDVVALP+SSGTTG+PKGVMLTHKSLVTSVAQQVDGENPN+   S DVI+C+LPLFHIY
Sbjct: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNLYSNDVIICVLPLFHIY 240

Query: 241 SLNSVLLCGLRVGAAILIIQKYDMSSLLHLIQAHKATVAPFVPPIVLDLAKNPDIHRYDL 300
           SLNSVLLCGLRVGAAILI++KYDMSSLL LIQ +KAT+APFVPPIVLD AKNPDIHRYDL
Sbjct: 241 SLNSVLLCGLRVGAAILILKKYDMSSLLPLIQTYKATIAPFVPPIVLDFAKNPDIHRYDL 300

Query: 301 SSIRIVMSGAAPLGKDLEDTLKARLPNANLGQGYGMTEAGPVLSMSLTFAKEPMAVKSGA 360
           SSIRIVMSGAAP+GKDLEDT+KARLPNA LGQGYGMTEAGPVLSM L FAKEP  VKSGA
Sbjct: 301 SSIRIVMSGAAPMGKDLEDTVKARLPNATLGQGYGMTEAGPVLSMCLAFAKEPFEVKSGA 360

Query: 361 CGTVVRNAEMKIIHPQTGASLPRNQPGEICIRGSQIMKGKYLNNKEATEETIDKDGWLHT 420
           CGTVVRNAEMKII+P+T  SLPRN PGEICIRGSQIMKG Y+NN+EATE+TIDKDGWLHT
Sbjct: 361 CGTVVRNAEMKIINPETSVSLPRNHPGEICIRGSQIMKG-YINNREATEKTIDKDGWLHT 420

Query: 421 GDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNIAAAAVIPMKDEAAGEI 480
           GDLGFIDD+DEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNI++AAVIPM+DEAAGEI
Sbjct: 421 GDLGFIDDNDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNISSAAVIPMEDEAAGEI 480

Query: 481 PVAFVVRSGGSKISEDEIKEYVSEQVIFYKKIRKVFLVDSIPIAPSGKILRKVLRDQLQA 540
           PVAFVVRS GSKISED++K+Y+S+QVI+YKKIRKVF VDSIPIAPSGKILRKVL+ QL+A
Sbjct: 481 PVAFVVRSKGSKISEDDLKKYISDQVIYYKKIRKVFFVDSIPIAPSGKILRKVLKAQLEA 540

Query: 541 GAF 543
           GAF
Sbjct: 541 GAF 542

BLAST of MC08g2118 vs. NCBI nr
Match: XP_022997404.1 (4-coumarate--CoA ligase 2-like [Cucurbita maxima])

HSP 1 Score: 916 bits (2367), Expect = 0.0
Identity = 453/543 (83.43%), Postives = 496/543 (91.34%), Query Frame = 0

Query: 1   MDSQIPTGDDFIFRSKLPDIYIPDHLPLHTYCFDRLSQFQHRPCLINAATGHVHTYAAVH 60
           MDSQ+    DFIFRSKLPDIYIP+HLPLHTYCF+ LSQF HRPCLINAATG +HTYAAV 
Sbjct: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60

Query: 61  LTARRVAAGLANIGISQGDVIMILLQNSPEFVFAFLGASYGGAISTTANPLYKPAEIAKQ 120
           L +RRVA GL+ IG+ QGDVIM+LLQNSPEFVFAFLGASY GAISTTANPLYKPAEI KQ
Sbjct: 61  LASRRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120

Query: 121 ARAANAKVIITQSAFVDKIKGFAEERGVKILCTDSPPDGCLSFSELTEADENDIPAVKIN 180
           A  A  KVIITQ+ FV+K++ FA E GVKI+CTDSPP GCL FSE+ EADEN+IPAVKIN
Sbjct: 121 AATARPKVIITQAEFVEKVREFAMENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180

Query: 181 SNDVVALPYSSGTTGIPKGVMLTHKSLVTSVAQQVDGENPNLYFKSKDVILCILPLFHIY 240
           SNDVVALP+SSGTTG+PKGVMLTHKSLVTS+AQQVDGENPN+   S DV++C+LPLFHIY
Sbjct: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240

Query: 241 SLNSVLLCGLRVGAAILIIQKYDMSSLLHLIQAHKATVAPFVPPIVLDLAKNPDIHRYDL 300
           SLNSVLLCGLRVGAAILI+QKYDMS+LL LIQ HKAT+APFVPPIVLD AKNPDIHRYDL
Sbjct: 241 SLNSVLLCGLRVGAAILILQKYDMSTLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300

Query: 301 SSIRIVMSGAAPLGKDLEDTLKARLPNANLGQGYGMTEAGPVLSMSLTFAKEPMAVKSGA 360
           SSIRIVMSGAAP+GK LEDT+KARLPNA LGQGYGMTEAGPVLSM L FAKEP   KSGA
Sbjct: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360

Query: 361 CGTVVRNAEMKIIHPQTGASLPRNQPGEICIRGSQIMKGKYLNNKEATEETIDKDGWLHT 420
           CGTVVRNAEMKIIHP+TG SLPRN PGEICIRGSQIMKG YLNNKEATE+TIDK+GWLHT
Sbjct: 361 CGTVVRNAEMKIIHPETGVSLPRNHPGEICIRGSQIMKG-YLNNKEATEKTIDKNGWLHT 420

Query: 421 GDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNIAAAAVIPMKDEAAGEI 480
           GDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPN+++AAV+PM+DEAAGEI
Sbjct: 421 GDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEI 480

Query: 481 PVAFVVRSGGSKISEDEIKEYVSEQVIFYKKIRKVFLVDSIPIAPSGKILRKVLRDQLQA 540
           P AFVVRS GSKISED+IK+Y+S+QVI+YKKIRKVF VDSIP+APSGKILRKVL+ QL+ 
Sbjct: 481 PAAFVVRSKGSKISEDDIKKYISDQVIYYKKIRKVFFVDSIPVAPSGKILRKVLKAQLEN 540

Query: 541 GAF 543
           GAF
Sbjct: 541 GAF 542

BLAST of MC08g2118 vs. NCBI nr
Match: XP_023546819.1 (4-coumarate--CoA ligase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 915 bits (2364), Expect = 0.0
Identity = 453/543 (83.43%), Postives = 495/543 (91.16%), Query Frame = 0

Query: 1   MDSQIPTGDDFIFRSKLPDIYIPDHLPLHTYCFDRLSQFQHRPCLINAATGHVHTYAAVH 60
           MDSQ+    DFIFRSKLPDIYIP+HLPLHTYCF  LSQF HRPCLINAATG +HTYAAV 
Sbjct: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFQHLSQFHHRPCLINAATGQIHTYAAVD 60

Query: 61  LTARRVAAGLANIGISQGDVIMILLQNSPEFVFAFLGASYGGAISTTANPLYKPAEIAKQ 120
           L ARRVA GL+ IG+ QGDVIM+LLQNSPEFVFAFLGASY GAISTTANPLYKPAEI KQ
Sbjct: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120

Query: 121 ARAANAKVIITQSAFVDKIKGFAEERGVKILCTDSPPDGCLSFSELTEADENDIPAVKIN 180
           A  A  KVIITQ+ FV+K++ FA E GVKI+CTDSPP GCL FSE+ EADEN+IPAVKIN
Sbjct: 121 AATARPKVIITQAEFVEKVREFALENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180

Query: 181 SNDVVALPYSSGTTGIPKGVMLTHKSLVTSVAQQVDGENPNLYFKSKDVILCILPLFHIY 240
           SNDVVALP+SSGTTG+PKGVMLTHKSLVTS+AQQVDGENPN+   S DV++C+LPLFHIY
Sbjct: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240

Query: 241 SLNSVLLCGLRVGAAILIIQKYDMSSLLHLIQAHKATVAPFVPPIVLDLAKNPDIHRYDL 300
           SLNSVLLCGLRVGAAILI+QKYDMSSLL LIQ HKAT+APFVPPIVLD AKNPDIHRYDL
Sbjct: 241 SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300

Query: 301 SSIRIVMSGAAPLGKDLEDTLKARLPNANLGQGYGMTEAGPVLSMSLTFAKEPMAVKSGA 360
           SSIRIVMSGAAP+GK LEDT+KARLPNA LGQGYGMTEAGPVLSM L FAKEP   KSGA
Sbjct: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360

Query: 361 CGTVVRNAEMKIIHPQTGASLPRNQPGEICIRGSQIMKGKYLNNKEATEETIDKDGWLHT 420
           CGTVVRNAEMKIIHP+TG SLPRN PGEICIRGSQIMKG YLNNKEATE+TIDK+GWLHT
Sbjct: 361 CGTVVRNAEMKIIHPETGVSLPRNHPGEICIRGSQIMKG-YLNNKEATEKTIDKNGWLHT 420

Query: 421 GDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNIAAAAVIPMKDEAAGEI 480
           GDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPN+++AAV+PM+DEAAGEI
Sbjct: 421 GDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEI 480

Query: 481 PVAFVVRSGGSKISEDEIKEYVSEQVIFYKKIRKVFLVDSIPIAPSGKILRKVLRDQLQA 540
           P AFVVR+ GSKISED+IK+Y+S+QVI+YKKIRKV  VDSIP+APSGKILRKVL+ QL++
Sbjct: 481 PAAFVVRTEGSKISEDDIKKYISDQVIYYKKIRKVLFVDSIPVAPSGKILRKVLKAQLES 540

Query: 541 GAF 543
           GAF
Sbjct: 541 GAF 542

BLAST of MC08g2118 vs. ExPASy TrEMBL
Match: A0A6J1DIM3 (4-coumarate--CoA ligase 1-like OS=Momordica charantia OX=3673 GN=LOC111020820 PE=4 SV=1)

HSP 1 Score: 1063 bits (2748), Expect = 0.0
Identity = 542/543 (99.82%), Postives = 542/543 (99.82%), Query Frame = 0

Query: 1   MDSQIPTGDDFIFRSKLPDIYIPDHLPLHTYCFDRLSQFQHRPCLINAATGHVHTYAAVH 60
           MDSQIPTGDDFIFRSKLPDIYIPDHLPLHTYCFDRLSQFQHRPCLINAATGHVHTYAAVH
Sbjct: 1   MDSQIPTGDDFIFRSKLPDIYIPDHLPLHTYCFDRLSQFQHRPCLINAATGHVHTYAAVH 60

Query: 61  LTARRVAAGLANIGISQGDVIMILLQNSPEFVFAFLGASYGGAISTTANPLYKPAEIAKQ 120
           LTARRVAAGLANIGISQGDVIMILLQNSPEFVFAFLGASYGGAISTTANPLYKPAEIAKQ
Sbjct: 61  LTARRVAAGLANIGISQGDVIMILLQNSPEFVFAFLGASYGGAISTTANPLYKPAEIAKQ 120

Query: 121 ARAANAKVIITQSAFVDKIKGFAEERGVKILCTDSPPDGCLSFSELTEADENDIPAVKIN 180
           ARAANAKVIITQSAFVDKIKGFAEERGVKILCTDSPPDGCLSFSELTEADENDIPAVKIN
Sbjct: 121 ARAANAKVIITQSAFVDKIKGFAEERGVKILCTDSPPDGCLSFSELTEADENDIPAVKIN 180

Query: 181 SNDVVALPYSSGTTGIPKGVMLTHKSLVTSVAQQVDGENPNLYFKSKDVILCILPLFHIY 240
           SNDVVALPYSSGTTGIPKGVMLTHKSLVTSVAQQVDGENPNLYFKSKDVILCILPLFHIY
Sbjct: 181 SNDVVALPYSSGTTGIPKGVMLTHKSLVTSVAQQVDGENPNLYFKSKDVILCILPLFHIY 240

Query: 241 SLNSVLLCGLRVGAAILIIQKYDMSSLLHLIQAHKATVAPFVPPIVLDLAKNPDIHRYDL 300
           SLNSVLLCGLRVGAAILIIQKYDMSSLLHLIQAHKATVAPFVPPIVLDLAKNPDIHRYDL
Sbjct: 241 SLNSVLLCGLRVGAAILIIQKYDMSSLLHLIQAHKATVAPFVPPIVLDLAKNPDIHRYDL 300

Query: 301 SSIRIVMSGAAPLGKDLEDTLKARLPNANLGQGYGMTEAGPVLSMSLTFAKEPMAVKSGA 360
           SSIRIVMSGAAPLGKDLEDTLKARLPNANLGQGYGMTEAGPVLSMSLTFAKEPMAVKSGA
Sbjct: 301 SSIRIVMSGAAPLGKDLEDTLKARLPNANLGQGYGMTEAGPVLSMSLTFAKEPMAVKSGA 360

Query: 361 CGTVVRNAEMKIIHPQTGASLPRNQPGEICIRGSQIMKGKYLNNKEATEETIDKDGWLHT 420
           CGTVVRNAEMKIIHPQTGASLPRNQPGEICIRGSQIMKG YLNNKEATEETIDKDGWLHT
Sbjct: 361 CGTVVRNAEMKIIHPQTGASLPRNQPGEICIRGSQIMKG-YLNNKEATEETIDKDGWLHT 420

Query: 421 GDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNIAAAAVIPMKDEAAGEI 480
           GDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNIAAAAVIPMKDEAAGEI
Sbjct: 421 GDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNIAAAAVIPMKDEAAGEI 480

Query: 481 PVAFVVRSGGSKISEDEIKEYVSEQVIFYKKIRKVFLVDSIPIAPSGKILRKVLRDQLQA 540
           PVAFVVRSGGSKISEDEIKEYVSEQVIFYKKIRKVFLVDSIPIAPSGKILRKVLRDQLQA
Sbjct: 481 PVAFVVRSGGSKISEDEIKEYVSEQVIFYKKIRKVFLVDSIPIAPSGKILRKVLRDQLQA 540

Query: 541 GAF 543
           GAF
Sbjct: 541 GAF 542

BLAST of MC08g2118 vs. ExPASy TrEMBL
Match: A0A6J1EP35 (4-coumarate--CoA ligase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111436262 PE=4 SV=1)

HSP 1 Score: 918 bits (2373), Expect = 0.0
Identity = 455/543 (83.79%), Postives = 496/543 (91.34%), Query Frame = 0

Query: 1   MDSQIPTGDDFIFRSKLPDIYIPDHLPLHTYCFDRLSQFQHRPCLINAATGHVHTYAAVH 60
           MDSQ+    DFIFRSKLPDIYIP+HLPLHTYCF+ LSQF HRPCLINAATG +HTYAAV 
Sbjct: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60

Query: 61  LTARRVAAGLANIGISQGDVIMILLQNSPEFVFAFLGASYGGAISTTANPLYKPAEIAKQ 120
           L ARRVA GL+ IG+ QGDVIM+LLQNSPEFVFAFLGASY GAISTTANPLYKPAEI KQ
Sbjct: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120

Query: 121 ARAANAKVIITQSAFVDKIKGFAEERGVKILCTDSPPDGCLSFSELTEADENDIPAVKIN 180
           A  A  KVIITQ+ FV+K++ FA E GVKI+CTDSPP GCL FSE+ EADEN+IPAVKIN
Sbjct: 121 AATARPKVIITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180

Query: 181 SNDVVALPYSSGTTGIPKGVMLTHKSLVTSVAQQVDGENPNLYFKSKDVILCILPLFHIY 240
           SNDVVALP+SSGTTG+PKGVMLTHKSLVTS+AQQVDGENPN+   S DV++C+LPLFHIY
Sbjct: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240

Query: 241 SLNSVLLCGLRVGAAILIIQKYDMSSLLHLIQAHKATVAPFVPPIVLDLAKNPDIHRYDL 300
           SLNSVLLCGLRVGAAILI+QKYDMSSLL LIQ HKAT+APFVPPIVLD AKNPDIHRYDL
Sbjct: 241 SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300

Query: 301 SSIRIVMSGAAPLGKDLEDTLKARLPNANLGQGYGMTEAGPVLSMSLTFAKEPMAVKSGA 360
           SSIRIVMSGAAP+GK LEDT+KARLPNA LGQGYGMTEAGPVLSM L FAKEP   KSGA
Sbjct: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360

Query: 361 CGTVVRNAEMKIIHPQTGASLPRNQPGEICIRGSQIMKGKYLNNKEATEETIDKDGWLHT 420
           CGTVVRNAEMKIIHP+TG SLPRN PGEICIRGSQIMKG YLNNKEATE+TIDK+GWLHT
Sbjct: 361 CGTVVRNAEMKIIHPETGVSLPRNHPGEICIRGSQIMKG-YLNNKEATEKTIDKNGWLHT 420

Query: 421 GDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNIAAAAVIPMKDEAAGEI 480
           GDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPN+++AAV+PM+DEAAGEI
Sbjct: 421 GDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEI 480

Query: 481 PVAFVVRSGGSKISEDEIKEYVSEQVIFYKKIRKVFLVDSIPIAPSGKILRKVLRDQLQA 540
           P AFVVRS GSKISED+IK+Y+S+QVI+YKKIRKVF VDSIP+APSGKILRKVL+ QL+ 
Sbjct: 481 PAAFVVRSKGSKISEDDIKKYISDQVIYYKKIRKVFFVDSIPVAPSGKILRKVLKAQLEN 540

Query: 541 GAF 543
           GAF
Sbjct: 541 GAF 542

BLAST of MC08g2118 vs. ExPASy TrEMBL
Match: A0A6J1KB99 (4-coumarate--CoA ligase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111492331 PE=4 SV=1)

HSP 1 Score: 916 bits (2367), Expect = 0.0
Identity = 453/543 (83.43%), Postives = 496/543 (91.34%), Query Frame = 0

Query: 1   MDSQIPTGDDFIFRSKLPDIYIPDHLPLHTYCFDRLSQFQHRPCLINAATGHVHTYAAVH 60
           MDSQ+    DFIFRSKLPDIYIP+HLPLHTYCF+ LSQF HRPCLINAATG +HTYAAV 
Sbjct: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60

Query: 61  LTARRVAAGLANIGISQGDVIMILLQNSPEFVFAFLGASYGGAISTTANPLYKPAEIAKQ 120
           L +RRVA GL+ IG+ QGDVIM+LLQNSPEFVFAFLGASY GAISTTANPLYKPAEI KQ
Sbjct: 61  LASRRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120

Query: 121 ARAANAKVIITQSAFVDKIKGFAEERGVKILCTDSPPDGCLSFSELTEADENDIPAVKIN 180
           A  A  KVIITQ+ FV+K++ FA E GVKI+CTDSPP GCL FSE+ EADEN+IPAVKIN
Sbjct: 121 AATARPKVIITQAEFVEKVREFAMENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180

Query: 181 SNDVVALPYSSGTTGIPKGVMLTHKSLVTSVAQQVDGENPNLYFKSKDVILCILPLFHIY 240
           SNDVVALP+SSGTTG+PKGVMLTHKSLVTS+AQQVDGENPN+   S DV++C+LPLFHIY
Sbjct: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240

Query: 241 SLNSVLLCGLRVGAAILIIQKYDMSSLLHLIQAHKATVAPFVPPIVLDLAKNPDIHRYDL 300
           SLNSVLLCGLRVGAAILI+QKYDMS+LL LIQ HKAT+APFVPPIVLD AKNPDIHRYDL
Sbjct: 241 SLNSVLLCGLRVGAAILILQKYDMSTLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300

Query: 301 SSIRIVMSGAAPLGKDLEDTLKARLPNANLGQGYGMTEAGPVLSMSLTFAKEPMAVKSGA 360
           SSIRIVMSGAAP+GK LEDT+KARLPNA LGQGYGMTEAGPVLSM L FAKEP   KSGA
Sbjct: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360

Query: 361 CGTVVRNAEMKIIHPQTGASLPRNQPGEICIRGSQIMKGKYLNNKEATEETIDKDGWLHT 420
           CGTVVRNAEMKIIHP+TG SLPRN PGEICIRGSQIMKG YLNNKEATE+TIDK+GWLHT
Sbjct: 361 CGTVVRNAEMKIIHPETGVSLPRNHPGEICIRGSQIMKG-YLNNKEATEKTIDKNGWLHT 420

Query: 421 GDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNIAAAAVIPMKDEAAGEI 480
           GDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPN+++AAV+PM+DEAAGEI
Sbjct: 421 GDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEI 480

Query: 481 PVAFVVRSGGSKISEDEIKEYVSEQVIFYKKIRKVFLVDSIPIAPSGKILRKVLRDQLQA 540
           P AFVVRS GSKISED+IK+Y+S+QVI+YKKIRKVF VDSIP+APSGKILRKVL+ QL+ 
Sbjct: 481 PAAFVVRSKGSKISEDDIKKYISDQVIYYKKIRKVFFVDSIPVAPSGKILRKVLKAQLEN 540

Query: 541 GAF 543
           GAF
Sbjct: 541 GAF 542

BLAST of MC08g2118 vs. ExPASy TrEMBL
Match: A0A1S3BSW8 (4-coumarate--CoA ligase 2-like OS=Cucumis melo OX=3656 GN=LOC103493360 PE=4 SV=1)

HSP 1 Score: 893 bits (2307), Expect = 0.0
Identity = 451/547 (82.45%), Postives = 489/547 (89.40%), Query Frame = 0

Query: 1   MDSQIPTGDDFIFRSKLPDIYIPDHLPLHTYCFDRLSQFQHRPCLINAATGHVHTYAAVH 60
           MD +   G DFIF+SKLPDIYIP+HLPLHTYCF+ L+QFQHRPCLIN  TG +HTYA V 
Sbjct: 1   MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD 60

Query: 61  LTARRVAAGLANIGISQGDVIMILLQNSPEFVFAFLGASYGGAISTTANPLYKPAEIAKQ 120
           L ARR AAGL+ IGI QGDVIM++LQNSPEFVFAFLGAS+ GAI+TTANPLYKP EI KQ
Sbjct: 61  LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ 120

Query: 121 ARAANAKVIITQSAFVDKIKGFAEERGVKILCTDSPPD----GCLSFSELTEADENDIPA 180
           A AA AKVIITQ  FV+K+  FA E GVKILCTDS       GCL FSE+ EADENDIPA
Sbjct: 121 AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA 180

Query: 181 VKINSNDVVALPYSSGTTGIPKGVMLTHKSLVTSVAQQVDGENPNLYFKSKDVILCILPL 240
           VKINSNDVVALP+SSGTTG+PKGVMLTHKSLVTSVAQQVDGENPN+   SKDVI+C+LPL
Sbjct: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL 240

Query: 241 FHIYSLNSVLLCGLRVGAAILIIQKYDMSSLLHLIQAHKATVAPFVPPIVLDLAKNPDIH 300
           FHIYSLNSVLLCGLRVGAAILI+QKYDMSSLL LIQ HKAT+APFVPPIVLD AKNPDIH
Sbjct: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH 300

Query: 301 RYDLSSIRIVMSGAAPLGKDLEDTLKARLPNANLGQGYGMTEAGPVLSMSLTFAKEPMAV 360
            Y+LSSIRIVMSGAAP+GKDLEDT+KARLPNA LGQGYGMTEAGPVLSM L FAKEP  V
Sbjct: 301 NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV 360

Query: 361 KSGACGTVVRNAEMKIIHPQTGASLPRNQPGEICIRGSQIMKGKYLNNKEATEETIDKDG 420
           KSGACGTVVRNAEMKIIHPQ+  SLPRN PGEICIRG QIMKG YLNN+EATE+TIDKDG
Sbjct: 361 KSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKG-YLNNEEATEKTIDKDG 420

Query: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNIAAAAVIPMKDEA 480
           WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNI +AAVIPM+DEA
Sbjct: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480

Query: 481 AGEIPVAFVVRSGGSKISEDEIKEYVSEQVIFYKKIRKVFLVDSIPIAPSGKILRKVLRD 540
           AGEIPVAFVVRS GSKISE++IK Y+S+QVI+YK+IRKVF VDSIP+APSGKILRKVL+ 
Sbjct: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKA 540

Query: 541 QLQAGAF 543
           QL+AG F
Sbjct: 541 QLEAGVF 546

BLAST of MC08g2118 vs. ExPASy TrEMBL
Match: A0A0A0LM95 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G048330 PE=4 SV=1)

HSP 1 Score: 886 bits (2290), Expect = 0.0
Identity = 445/541 (82.26%), Postives = 485/541 (89.65%), Query Frame = 0

Query: 7   TGDDFIFRSKLPDIYIPDHLPLHTYCFDRLSQFQHRPCLINAATGHVHTYAAVHLTARRV 66
            G DFIF+SKLPDIYIP+HLPLHTYCF+ LSQFQHRPCLIN  TG +HTYA V+L ARR 
Sbjct: 8   NGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEVNLAARRF 67

Query: 67  AAGLANIGISQGDVIMILLQNSPEFVFAFLGASYGGAISTTANPLYKPAEIAKQARAANA 126
           AAGL+ IG+ QGDVIM++LQNSPEFVFAFLGAS+ GAI+TTANPLYKP EI KQA AA  
Sbjct: 68  AAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQAVAAKV 127

Query: 127 KVIITQSAFVDKIKGFAEERGVKILCTDSPPD----GCLSFSELTEADENDIPAVKINSN 186
           KVIITQ  FV+K+  FA E GVKILCTDS       GCL FSE+ EADEN+IPAVKINS+
Sbjct: 128 KVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIPAVKINSS 187

Query: 187 DVVALPYSSGTTGIPKGVMLTHKSLVTSVAQQVDGENPNLYFKSKDVILCILPLFHIYSL 246
           DVVALP+SSGTTG+PKGVMLTHKSLVTSVAQQVDGENPN+    KDVI+C+LPLFHIYSL
Sbjct: 188 DVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLPLFHIYSL 247

Query: 247 NSVLLCGLRVGAAILIIQKYDMSSLLHLIQAHKATVAPFVPPIVLDLAKNPDIHRYDLSS 306
           NSVLLCGLRVGAAILI+QKYD+SSLL LIQ HKAT+APFVPPIVLD AKNPDIHRYDLSS
Sbjct: 248 NSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSS 307

Query: 307 IRIVMSGAAPLGKDLEDTLKARLPNANLGQGYGMTEAGPVLSMSLTFAKEPMAVKSGACG 366
           IRIVMSGAAP+GKDLEDT+KA+LPNA LGQGYGMTEAGPVLSM L FAKE   VKSGACG
Sbjct: 308 IRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFKVKSGACG 367

Query: 367 TVVRNAEMKIIHPQTGASLPRNQPGEICIRGSQIMKGKYLNNKEATEETIDKDGWLHTGD 426
           TVVRNAEMKIIHPQT  SLPRN PGEICIRG QIMKG YLNNKEATE+TIDKDGWLHTGD
Sbjct: 368 TVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKG-YLNNKEATEKTIDKDGWLHTGD 427

Query: 427 LGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNIAAAAVIPMKDEAAGEIPV 486
           LG+IDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNI +AAVIPM+DEAAGEIPV
Sbjct: 428 LGYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAAGEIPV 487

Query: 487 AFVVRSGGSKISEDEIKEYVSEQVIFYKKIRKVFLVDSIPIAPSGKILRKVLRDQLQAGA 543
           AFVVRS GSKISE++IK Y+S+QVI+YKKIRKVF VDSIP+APSGKILRK+L+ QL+AG 
Sbjct: 488 AFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKAQLEAGV 547

BLAST of MC08g2118 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 728.0 bits (1878), Expect = 5.5e-210
Identity = 364/540 (67.41%), Postives = 440/540 (81.48%), Query Frame = 0

Query: 10  DFIFRSKLPDIYIPDHLPLHTYCFDRLSQFQHRPCLINAATGHVHTYAAVHLTARRVAAG 69
           D IFRSKLPDIYIP+HL LH Y F  +S+F  +PCLIN  TGHV+TY+ VH+ +R++AA 
Sbjct: 22  DVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIAAN 81

Query: 70  LANIGISQGDVIMILLQNSPEFVFAFLGASYGGAISTTANPLYKPAEIAKQARAANAKVI 129
              +G++Q DV+M+LL N PEFV +FL AS+ GA +T ANP + PAEIAKQA+A+N K+I
Sbjct: 82  FHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTKLI 141

Query: 130 ITQSAFVDKIKGFAEERGVKILCTDSP-----PDGCLSFSELTEAD---ENDIPAVKINS 189
           IT++ +VDKIK    + GV I+C D       P+GCL F+ELT++       I +V+I+ 
Sbjct: 142 ITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEISP 201

Query: 190 NDVVALPYSSGTTGIPKGVMLTHKSLVTSVAQQVDGENPNLYFKSKDVILCILPLFHIYS 249
           +DVVALPYSSGTTG+PKGVMLTHK LVTSVAQQVDGENPNLYF S DVILC+LP+FHIY+
Sbjct: 202 DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHIYA 261

Query: 250 LNSVLLCGLRVGAAILIIQKYDMSSLLHLIQAHKATVAPFVPPIVLDLAKNPDIHRYDLS 309
           LNS++LCGLRVGAAILI+ K++++ LL LIQ  K TVAP VPPIVL +AK+ +  +YDLS
Sbjct: 262 LNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKYDLS 321

Query: 310 SIRIVMSGAAPLGKDLEDTLKARLPNANLGQGYGMTEAGPVLSMSLTFAKEPMAVKSGAC 369
           SIR+V SGAAPLGK+LED + A+ PNA LGQGYGMTEAGPVL+MSL FAKEP  VKSGAC
Sbjct: 322 SIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGAC 381

Query: 370 GTVVRNAEMKIIHPQTGASLPRNQPGEICIRGSQIMKGKYLNNKEATEETIDKDGWLHTG 429
           GTVVRNAEMKI+ P TG SL RNQPGEICIRG QIMKG YLNN  AT ETIDKDGWLHTG
Sbjct: 382 GTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKG-YLNNPAATAETIDKDGWLHTG 441

Query: 430 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNIAAAAVIPMKDEAAGEIP 489
           D+G IDDDDE+FIVDRLKELIKYKG+QVAPAELEALL  +P+I   AV+ MK+EAAGE+P
Sbjct: 442 DIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGEVP 501

Query: 490 VAFVVRSGGSKISEDEIKEYVSEQVIFYKKIRKVFLVDSIPIAPSGKILRKVLRDQLQAG 542
           VAFVV+S  S++SED++K++VS+QV+FYK+I KVF  +SIP APSGKILRK LR +L  G
Sbjct: 502 VAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKLANG 560

BLAST of MC08g2118 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 726.5 bits (1874), Expect = 1.6e-209
Identity = 368/544 (67.65%), Postives = 444/544 (81.62%), Query Frame = 0

Query: 2   DSQIPTGDDFIFRSKLPDIYIPDHLPLHTYCFDRLSQFQHRPCLINAATGHVHTYAAVHL 61
           + Q    +D IFRS+LPDIYIP+HLPLH Y F+ +S+F  +PCLIN  TG V+TYA VH+
Sbjct: 12  NDQKQCSNDVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHV 71

Query: 62  TARRVAAGLANIGISQGDVIMILLQNSPEFVFAFLGASYGGAISTTANPLYKPAEIAKQA 121
           T+R++AAGL N+G+ Q DV+MILL NSPE V  FL AS+ GAI+T+ANP + PAEI+KQA
Sbjct: 72  TSRKLAAGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQA 131

Query: 122 RAANAKVIITQSAFVDKIKGFAEERGVKILCTDSP--PDGCLSFSELTEADENDIPAV-- 181
           +A+ AK+I+TQS +VDKIK    + GV I+ TDS   P+ CL FSELT+++E  + ++  
Sbjct: 132 KASAAKLIVTQSRYVDKIKNLQND-GVLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPE 191

Query: 182 KINSNDVVALPYSSGTTGIPKGVMLTHKSLVTSVAQQVDGENPNLYFKSKDVILCILPLF 241
           KI+  DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGENPNLYF   DVILC+LP+F
Sbjct: 192 KISPEDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMF 251

Query: 242 HIYSLNSVLLCGLRVGAAILIIQKYDMSSLLHLIQAHKATVAPFVPPIVLDLAKNPDIHR 301
           HIY+LNS++LC LRVGA ILI+ K++++ LL  IQ  K TVA  VPPIVL +AK+P+  +
Sbjct: 252 HIYALNSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEK 311

Query: 302 YDLSSIRIVMSGAAPLGKDLEDTLKARLPNANLGQGYGMTEAGPVLSMSLTFAKEPMAVK 361
           YDLSS+R+V SGAAPLGK+LED + A+ PNA LGQGYGMTEAGPVL+MSL FAKEP  VK
Sbjct: 312 YDLSSVRMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVK 371

Query: 362 SGACGTVVRNAEMKIIHPQTGASLPRNQPGEICIRGSQIMKGKYLNNKEATEETIDKDGW 421
           SGACGTVVRNAEMKI+ P TG SLPRN+PGEICIRG+QIMKG YLN+  AT  TIDKDGW
Sbjct: 372 SGACGTVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKG-YLNDPLATASTIDKDGW 431

Query: 422 LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNIAAAAVIPMKDEAA 481
           LHTGD+GFIDDDDE+FIVDRLKELIKYKG+QVAPAELE+LL  +P I   AV+ MK+E A
Sbjct: 432 LHTGDVGFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDA 491

Query: 482 GEIPVAFVVRSGGSKISEDEIKEYVSEQVIFYKKIRKVFLVDSIPIAPSGKILRKVLRDQ 541
           GE+PVAFVVRS  S ISEDEIK++VS+QV+FYK+I KVF  DSIP APSGKILRK LR +
Sbjct: 492 GEVPVAFVVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRAR 551

BLAST of MC08g2118 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 684.9 bits (1766), Expect = 5.4e-197
Identity = 341/505 (67.52%), Postives = 413/505 (81.78%), Query Frame = 0

Query: 10  DFIFRSKLPDIYIPDHLPLHTYCFDRLSQFQHRPCLINAATGHVHTYAAVHLTARRVAAG 69
           D IFRSKLPDIYIP+HL LH Y F  +S+F  +PCLIN  TGHV+TY+ VH+ +R++AA 
Sbjct: 22  DVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIAAN 81

Query: 70  LANIGISQGDVIMILLQNSPEFVFAFLGASYGGAISTTANPLYKPAEIAKQARAANAKVI 129
              +G++Q DV+M+LL N PEFV +FL AS+ GA +T ANP + PAEIAKQA+A+N K+I
Sbjct: 82  FHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTKLI 141

Query: 130 ITQSAFVDKIKGFAEERGVKILCTDSP-----PDGCLSFSELTEAD---ENDIPAVKINS 189
           IT++ +VDKIK    + GV I+C D       P+GCL F+ELT++       I +V+I+ 
Sbjct: 142 ITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEISP 201

Query: 190 NDVVALPYSSGTTGIPKGVMLTHKSLVTSVAQQVDGENPNLYFKSKDVILCILPLFHIYS 249
           +DVVALPYSSGTTG+PKGVMLTHK LVTSVAQQVDGENPNLYF S DVILC+LP+FHIY+
Sbjct: 202 DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHIYA 261

Query: 250 LNSVLLCGLRVGAAILIIQKYDMSSLLHLIQAHKATVAPFVPPIVLDLAKNPDIHRYDLS 309
           LNS++LCGLRVGAAILI+ K++++ LL LIQ  K TVAP VPPIVL +AK+ +  +YDLS
Sbjct: 262 LNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKYDLS 321

Query: 310 SIRIVMSGAAPLGKDLEDTLKARLPNANLGQGYGMTEAGPVLSMSLTFAKEPMAVKSGAC 369
           SIR+V SGAAPLGK+LED + A+ PNA LGQGYGMTEAGPVL+MSL FAKEP  VKSGAC
Sbjct: 322 SIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGAC 381

Query: 370 GTVVRNAEMKIIHPQTGASLPRNQPGEICIRGSQIMKGKYLNNKEATEETIDKDGWLHTG 429
           GTVVRNAEMKI+ P TG SL RNQPGEICIRG QIMKG YLNN  AT ETIDKDGWLHTG
Sbjct: 382 GTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKG-YLNNPAATAETIDKDGWLHTG 441

Query: 430 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNIAAAAVIPMKDEAAGEIP 489
           D+G IDDDDE+FIVDRLKELIKYKG+QVAPAELEALL  +P+I   AV+ MK+EAAGE+P
Sbjct: 442 DIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGEVP 501

Query: 490 VAFVVRSGGSKISEDEIKEYVSEQV 507
           VAFVV+S  S++SED++K++VS+QV
Sbjct: 502 VAFVVKSKDSELSEDDVKQFVSKQV 525

BLAST of MC08g2118 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 681.8 bits (1758), Expect = 4.5e-196
Identity = 340/530 (64.15%), Postives = 426/530 (80.38%), Query Frame = 0

Query: 12  IFRSKLPDIYIPDHLPLHTYCFDRLSQFQHRPCLINAATGHVHTYAAVHLTARRVAAGLA 71
           IFRSKLPDI IP+HLPLHTYCF++LS    +PCLI  +TG  +TY   HL  RRVA+GL 
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 72  NIGISQGDVIMILLQNSPEFVFAFLGASYGGAISTTANPLYKPAEIAKQARAANAKVIIT 131
            +GI +GDVIMILLQNS EFVF+F+GAS  GA+STTANP Y   E+ KQ +++ AK+IIT
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 132 QSAFVDKIKGFAEERGVKILCTDSP-PDGCLSFSELTEADEND--IPAVKINSNDVVALP 191
            S +VDK+K   E   + ++ TD P P+ CL FS L   DE +     V I  +D  ALP
Sbjct: 154 HSQYVDKLKNLGE--NLTLITTDEPTPENCLPFSTLITDDETNPFQETVDIGGDDAAALP 213

Query: 192 YSSGTTGIPKGVMLTHKSLVTSVAQQVDGENPNLYFKSKDVILCILPLFHIYSLNSVLLC 251
           +SSGTTG+PKGV+LTHKSL+TSVAQQVDG+NPNLY KS DVILC+LPLFHIYSLNSVLL 
Sbjct: 214 FSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLN 273

Query: 252 GLRVGAAILIIQKYDMSSLLHLIQAHKATVAPFVPPIVLDLAKNPDIHRYDLSSIRIVMS 311
            LR GA +L++ K+++ +LL LIQ H+ T+A  VPP+V+ LAKNP ++ YDLSS+R V+S
Sbjct: 274 SLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRFVLS 333

Query: 312 GAAPLGKDLEDTLKARLPNANLGQGYGMTEAGPVLSMSLTFAKEPMAVKSGACGTVVRNA 371
           GAAPLGK+L+D+L+ RLP A LGQGYGMTEAGPVLSMSL FAKEP+  KSG+CGTVVRNA
Sbjct: 334 GAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVRNA 393

Query: 372 EMKIIHPQTGASLPRNQPGEICIRGSQIMKGKYLNNKEATEETIDKDGWLHTGDLGFIDD 431
           E+K++H +T  SL  NQPGEICIRG QIMK +YLN+ EAT  TID++GWLHTGD+G++D+
Sbjct: 394 ELKVVHLETRLSLGYNQPGEICIRGQQIMK-EYLNDPEATSATIDEEGWLHTGDIGYVDE 453

Query: 432 DDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNIAAAAVIPMKDEAAGEIPVAFVVRS 491
           DDEIFIVDRLKE+IK+KG+QV PAELE+LL ++ +IA AAV+P  DE AGE+PVAFVVRS
Sbjct: 454 DDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVRS 513

Query: 492 GGSKISEDEIKEYVSEQVIFYKKIRKVFLVDSIPIAPSGKILRKVLRDQL 539
            G+ I+E+++KEYV++QV+FYK++ KVF V SIP +PSGKILRK L+ +L
Sbjct: 514 NGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of MC08g2118 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 647.1 bits (1668), Expect = 1.2e-185
Identity = 325/551 (58.98%), Postives = 416/551 (75.50%), Query Frame = 0

Query: 2   DSQIPTGDDFIFRSKLPDIYIPDHLPLHTYCFDRLS----QFQHRPCLINAATGHVHTYA 61
           D +     DFIFRSKLPDI+IP+HLPL  Y F R S          C+I+ ATG + TYA
Sbjct: 18  DEEEEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYA 77

Query: 62  AVHLTARRVAAGLANIGISQGDVIMILLQNSPEFVFAFLGASYGGAISTTANPLYKPAEI 121
            V    RR+AAG+  +GI  GDV+M+LL NSPEF  +FL  +Y GA+STTANP Y   EI
Sbjct: 78  DVQTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEI 137

Query: 122 AKQARAANAKVIITQSAFVDKIKGFAEERGVKILCTD---------SPPDGCLSFSELTE 181
           AKQA+A+ AK+IIT+   VDK+     + GV I+C D         S  DGC+SF+ELT+
Sbjct: 138 AKQAKASAAKMIITKKCLVDKLTNLKND-GVLIVCLDDDGDNGVVSSSDDGCVSFTELTQ 197

Query: 182 ADENDIPAVKINSNDVVALPYSSGTTGIPKGVMLTHKSLVTSVAQQVDGENPNLYFKSKD 241
           ADE ++   KI+  D VA+PYSSGTTG+PKGVM+THK LVTS+AQ+VDGENPNL F + D
Sbjct: 198 ADETELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTAND 257

Query: 242 VILCILPLFHIYSLNSVLLCGLRVGAAILIIQKYDMSSLLHLIQAHKATVAPFVPPIVLD 301
           VILC LP+FHIY+L++++L  +R GAA+LI+ +++++ ++ LIQ +K TV P  PP+VL 
Sbjct: 258 VILCFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLA 317

Query: 302 LAKNPDIHRYDLSSIRIVMSGAAPLGKDLEDTLKARLPNANLGQGYGMTEAGPVLSMSLT 361
             K+P+  RYDLSS+RI++SGAA L K+LED ++ + PNA  GQGYGMTE+G V + SL 
Sbjct: 318 FIKSPETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLA 377

Query: 362 FAKEPMAVKSGACGTVVRNAEMKIIHPQTGASLPRNQPGEICIRGSQIMKGKYLNNKEAT 421
           FAK P   KSGACGTV+RNAEMK++  +TG SLPRN+ GEIC+RG Q+MKG YLN+ EAT
Sbjct: 378 FAKNPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKG-YLNDPEAT 437

Query: 422 EETIDKDGWLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNIAAAA 481
             TIDKDGWLHTGD+GF+DDDDEIFIVDRLKELIK+KGYQVAPAELEALL S+P+I  AA
Sbjct: 438 ARTIDKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAA 497

Query: 482 VIPMKDEAAGEIPVAFVVRSGGSKISEDEIKEYVSEQVIFYKKIRKVFLVDSIPIAPSGK 540
           V+ MKDE A E+PVAFV RS GS+++ED++K YV++QV+ YK+I+ VF ++ IP A SGK
Sbjct: 498 VVAMKDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGK 557