MC08g1814 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g1814
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionMannose-6-phosphate isomerase
LocationMC08: 26314856 .. 26318597 (+)
RNA-Seq ExpressionMC08g1814
SyntenyMC08g1814
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CACGGTGAATTGTTAATTCAAAGAAACGCAGGGAAAAAAAAAACTGTAAAAAGTCAACATTTTGACTCTCTCTAACGTCGTCTATACTGTTTCTCTCTCCTAGTCGCCGTCTCGAACTTATTTCCCTCTCTGCCTGACTCCTTCAAAAACCAGCTAAATTCCTTTTCCTTTTTCCTCTCCCATGGCGGACCTCAATTTATACCCCTTCAACATTCTCTGCTAAATTCCCACAACTTCCTTCTCGTTCATCATCATCATCTTCTTCTTCTTCCTCTCTCTCTGTACTTGATGGAGTCCGAGGCTGTTTTGAGGACGAAGCAGAGCAATGTCTTCCGATTGAAGTGCTCCGTCCAGAACTACGATTGGGGGATCAGAGGACCGGATTCTGCAGTCGCGAGGCTCTTTGCGTTGAACTCTGGCTCGATTGTCGATTCCGATAAGCCCTACGCGGAGTTTTGGATGGGGACTCACGATTCCGGACCTTCCTTTCTGATCGGCGCCGCTGAGAATGGCGGTGCGGTTGAGTCCGATTCCACTTCCCTCAAGTCTTGGGTTTCTGAAAACCCTAATTCCCTCGGCGAGAAGGTCGTCGAGAAATGGGGCTCCGACCTCCCCTTCCTGTTCAAGGTACGCTGCGGTACGAATTGATTTGCCGAAAACGCGTTTGTTAACCAAACGAATGGGTTTCGAATTTTCTCGTTTCGATTTCTGTTTTCGTTTGTTTTAGGTTCTCTCTGTGGCGAAAGCATTGTCGATACAGGCTCATCCGGACAAGGAATTGGCGAAGATACTCCATAAGTTGCAACCCAAAGCTTATAGAGACGCAAATCACAAGCCGGAAATGGCACTGGCGATTACGGAGTTCGAAGCTCTCTGTGGATTCATCAGTCTCGAGGTAAGAGTAGTTTACCCATTTTCCCTCTCATCGGGAACCTCGAATCTTCCAGATGATCTGGGAGATGCGTGGATGAGTATTAAAGTTCCATGGATTCGTTTGTTTTAAGATTTGCCACATTTCCTGGAGGCTTTCGATGATTATTTTATTGGGTTCCCCTTGATTTTGTAGTCTCCACTTTTATAAGTTTGTTTGAATTTTTTTAAGTTGAACTTGGAATTTTACCTGATTTCCTAATCTTGATCTTTACTTTTCTTTTGTTGTTTAATGAATACTATTCGTTGGGAACTGTGAAATATTATATTGTAAAGCTCTCTGTCGTCAGGCTTACGTTCAAATCCTCCGTTTACTTTTTTTTTTCTTTGACAAAACTTTCTTTGGACTTGTTAATCTTTGTCTGATATGACAAGTAGCCAAATATTTTATGTTTATTGGTCCAGTTGAATTCGTTCCTCGTGCTTTATTATTGGAATGTCGTTGTATATCCTTGATTTGTGGTTTAATGTATTGTGGTCCCTTTAGCAGCAACATAGCTTTTAACTTTTTATGACTTTTCAGAAGTCTCGAGTGAGGAAGGAAAACTGGGGAGACAAAATGTTGGAAGTTAATGTTTAGCATAATATGTTATAAATTTCCATATTATTTGCTAATTTTCTGGGATAGCTTGCAAGATTTAACGAGGGAATAGATGGATTTTGTGAAACAAATAGTTCTCCTTCTGAGTTTGAGAGAACTTTTTTGTCTCAGGTTTCTCAGCTGCTGTTTTTTTTTAAAGAATGTCCTTGGTTCTCTCTTCCCATGTTGAAAAGAAAATTTTGTTTCTCTTCATGTTTAGACTTTAGACGACTTCATCTTCTGTTTTCTTTACTTTCCAGGAGCTTAAGGATGTTCTTCGTTCTGTTCCTGAGACTGTGGAAATGATTGGCCATGAAGACACAAACAGAGTGCTGTTATTAGATGATGAAGATGAGGAAGAGGACCTGAAGTCTGTCCTGAGGTCAGCTTTCACTCAGTTAATGTCGGCTACCAAATCAATGATATCGGGCACGATATCCGACTTGAAGAGGCGGCTGCACTCTGAAAGTGAGGTAACTATGCAATGGTTTATTTGATTATTCAACAAGGGTGTACTGAAGAAGGAAGGTACCCCAGCTGATCTCAGTTTAACAAATTACAGAAAATGAAAGTTAAGCCTTTAAAGTTTTCTCTAATTTACCCCCTAAATTTTTTAAAATTAAATGATTATATATAATAAAGTTAGAAGGAGAAAGTCTTCCCCTCCCTGAAAAAAGAAAATTCTGATCCCCTGTTTTGAAGGAAGCTTTGTTAAAGTGATCCACAATTTTCACCTATAGAAATTTGAATGTAGAAATCAAAGTTTATCTCTGGCCATCACCCTTCATTATTCTATTACATAGATCAGGGCCGGGAATTCTTTGCAGCAATGAAGAATTTAATTATATTTGCAGCTTCTCTGTAGTATTGTTTTCTTTGAAAGAAGATAATAAATAACATCTATTTTGACCACAATGACTTTGTTTTATTTCTGCTACATGGAAAGCATGAATATGAGCCATGTCACTGTGAATGAAATTTCTAGTTTTTTATTAGATTTCATTCGAGATTGAATGTCATGTTTGAAGTACACTTTTTGATGGAGCTCTTACCCACTTGTTGAGTAGTGTCTATATTTGAAGCAAGATTTTAAAGGTCAATGTCAACGGAAATTTCGAGATCTCTATTTTAAGAAATTGTCGATGAAAATATGAATAAAACATTGATGTTGATGAAATTTAATACCTCGATTTGGAGTACACTTGCTCCCTGACTTTTGGAGTAAGATTTATGATCTGTGCTCATCTAGCTGCCCTGTTTTATTAATTTAATCTTTTCTTCAGGTGAGGCAGCTAACTGCTAAGGAACTCCTAGTATTACGTTTGGAGAAGCAATATCCTGATGATGTGGGCGTTATTGCAGCATTCTTCTTAAATTATGTGAAGCTGAATCCTGGAGAAGCTCTGTACCTCGGAGCAAACGAGCCACATGCATATATAAGTGGCGAGTGTATCGAGTGCATGGCAACGTCTGATAATGTCGTCCGGGCTGGCCTAACTCCCAAGTTTCGCGATGTCCAGACTCTCTGTGCTATGCTTACTTACAAACTGGTAAAACTTTTGTGCCCAATTACATCCATTTCTTTGTAAGTTTGATATCAAGTTCATGAAGCAACATGAAGTTCTTGGATGTTCATCCATCGATAAATCAAAAAAGTTTGCAAATGTGAAAGGGAGTCTAAAAGTTATGACATGTTTATGAGATTTGATCTGTGCAGGGCTTTCCTGAAATCCTCCAAGGAGTTCCTGTAAATCCATACATAACAAAGTACATTCCACCGTTCGATGAATTTGAGGTTGACTGTTGCGTTCTTCCTGAGGGAGCATCCGTCTTATTTCCAGCAAGCCCCGGTCCTTCGATATGCTTGGTTGCTCGGGGGGAGGGGATGATGCTGCCTCTAGAATCACTAGAGAGTGATGTAATCAGTGAAGGAGATGTCCTCTTTGTAGCTGCTAAAACTGAGATTAGTATAACGAGTGAAGTTGAGTTGCTCATTTTCAGAACGGGAGTAAACAGCAGGTTCTTTGATGCATTCATGAACGGTAAATAAAATATTCACTTTGATTCATGAATGGTAAATAAAATATTCACCTTGATTTTTTTTCCCTATATGGTATCCTATAAATATGTATTTTTCTTTTTTCCATTCGGTGTATTAGCTTGTACATAAGAAACTATGTTTTTTCTAACAGTAATAATAATAATATTATTATTATTTGAGTTGAACGTGCG

mRNA sequence

CACGGTGAATTGTTAATTCAAAGAAACGCAGGGAAAAAAAAAACTGTAAAAAGTCAACATTTTGACTCTCTCTAACGTCGTCTATACTGTTTCTCTCTCCTAGTCGCCGTCTCGAACTTATTTCCCTCTCTGCCTGACTCCTTCAAAAACCAGCTAAATTCCTTTTCCTTTTTCCTCTCCCATGGCGGACCTCAATTTATACCCCTTCAACATTCTCTGCTAAATTCCCACAACTTCCTTCTCGTTCATCATCATCATCTTCTTCTTCTTCCTCTCTCTCTGTACTTGATGGAGTCCGAGGCTGTTTTGAGGACGAAGCAGAGCAATGTCTTCCGATTGAAGTGCTCCGTCCAGAACTACGATTGGGGGATCAGAGGACCGGATTCTGCAGTCGCGAGGCTCTTTGCGTTGAACTCTGGCTCGATTGTCGATTCCGATAAGCCCTACGCGGAGTTTTGGATGGGGACTCACGATTCCGGACCTTCCTTTCTGATCGGCGCCGCTGAGAATGGCGGTGCGGTTGAGTCCGATTCCACTTCCCTCAAGTCTTGGGTTTCTGAAAACCCTAATTCCCTCGGCGAGAAGGTCGTCGAGAAATGGGGCTCCGACCTCCCCTTCCTGTTCAAGGTTCTCTCTGTGGCGAAAGCATTGTCGATACAGGCTCATCCGGACAAGGAATTGGCGAAGATACTCCATAAGTTGCAACCCAAAGCTTATAGAGACGCAAATCACAAGCCGGAAATGGCACTGGCGATTACGGAGTTCGAAGCTCTCTGTGGATTCATCAGTCTCGAGGAGCTTAAGGATGTTCTTCGTTCTGTTCCTGAGACTGTGGAAATGATTGGCCATGAAGACACAAACAGAGTGCTGTTATTAGATGATGAAGATGAGGAAGAGGACCTGAAGTCTGTCCTGAGGTCAGCTTTCACTCAGTTAATGTCGGCTACCAAATCAATGATATCGGGCACGATATCCGACTTGAAGAGGCGGCTGCACTCTGAAAGTGAGGTGAGGCAGCTAACTGCTAAGGAACTCCTAGTATTACGTTTGGAGAAGCAATATCCTGATGATGTGGGCGTTATTGCAGCATTCTTCTTAAATTATGTGAAGCTGAATCCTGGAGAAGCTCTGTACCTCGGAGCAAACGAGCCACATGCATATATAAGTGGCGAGTGTATCGAGTGCATGGCAACGTCTGATAATGTCGTCCGGGCTGGCCTAACTCCCAAGTTTCGCGATGTCCAGACTCTCTGTGCTATGCTTACTTACAAACTGGGCTTTCCTGAAATCCTCCAAGGAGTTCCTGTAAATCCATACATAACAAAGTACATTCCACCGTTCGATGAATTTGAGGTTGACTGTTGCGTTCTTCCTGAGGGAGCATCCGTCTTATTTCCAGCAAGCCCCGGTCCTTCGATATGCTTGGTTGCTCGGGGGGAGGGGATGATGCTGCCTCTAGAATCACTAGAGAGTGATGTAATCAGTGAAGGAGATGTCCTCTTTGTAGCTGCTAAAACTGAGATTAGTATAACGAGTGAAGTTGAGTTGCTCATTTTCAGAACGGGAGTAAACAGCAGGTTCTTTGATGCATTCATGAACGGTAAATAAAATATTCACTTTGATTCATGAATGGTAAATAAAATATTCACCTTGATTTTTTTTCCCTATATGGTATCCTATAAATATGTATTTTTCTTTTTTCCATTCGGTGTATTAGCTTGTACATAAGAAACTATGTTTTTTCTAACAGTAATAATAATAATATTATTATTATTTGAGTTGAACGTGCG

Coding sequence (CDS)

ATGGAGTCCGAGGCTGTTTTGAGGACGAAGCAGAGCAATGTCTTCCGATTGAAGTGCTCCGTCCAGAACTACGATTGGGGGATCAGAGGACCGGATTCTGCAGTCGCGAGGCTCTTTGCGTTGAACTCTGGCTCGATTGTCGATTCCGATAAGCCCTACGCGGAGTTTTGGATGGGGACTCACGATTCCGGACCTTCCTTTCTGATCGGCGCCGCTGAGAATGGCGGTGCGGTTGAGTCCGATTCCACTTCCCTCAAGTCTTGGGTTTCTGAAAACCCTAATTCCCTCGGCGAGAAGGTCGTCGAGAAATGGGGCTCCGACCTCCCCTTCCTGTTCAAGGTTCTCTCTGTGGCGAAAGCATTGTCGATACAGGCTCATCCGGACAAGGAATTGGCGAAGATACTCCATAAGTTGCAACCCAAAGCTTATAGAGACGCAAATCACAAGCCGGAAATGGCACTGGCGATTACGGAGTTCGAAGCTCTCTGTGGATTCATCAGTCTCGAGGAGCTTAAGGATGTTCTTCGTTCTGTTCCTGAGACTGTGGAAATGATTGGCCATGAAGACACAAACAGAGTGCTGTTATTAGATGATGAAGATGAGGAAGAGGACCTGAAGTCTGTCCTGAGGTCAGCTTTCACTCAGTTAATGTCGGCTACCAAATCAATGATATCGGGCACGATATCCGACTTGAAGAGGCGGCTGCACTCTGAAAGTGAGGTGAGGCAGCTAACTGCTAAGGAACTCCTAGTATTACGTTTGGAGAAGCAATATCCTGATGATGTGGGCGTTATTGCAGCATTCTTCTTAAATTATGTGAAGCTGAATCCTGGAGAAGCTCTGTACCTCGGAGCAAACGAGCCACATGCATATATAAGTGGCGAGTGTATCGAGTGCATGGCAACGTCTGATAATGTCGTCCGGGCTGGCCTAACTCCCAAGTTTCGCGATGTCCAGACTCTCTGTGCTATGCTTACTTACAAACTGGGCTTTCCTGAAATCCTCCAAGGAGTTCCTGTAAATCCATACATAACAAAGTACATTCCACCGTTCGATGAATTTGAGGTTGACTGTTGCGTTCTTCCTGAGGGAGCATCCGTCTTATTTCCAGCAAGCCCCGGTCCTTCGATATGCTTGGTTGCTCGGGGGGAGGGGATGATGCTGCCTCTAGAATCACTAGAGAGTGATGTAATCAGTGAAGGAGATGTCCTCTTTGTAGCTGCTAAAACTGAGATTAGTATAACGAGTGAAGTTGAGTTGCTCATTTTCAGAACGGGAGTAAACAGCAGGTTCTTTGATGCATTCATGAACGGTAAATAA

Protein sequence

MESEAVLRTKQSNVFRLKCSVQNYDWGIRGPDSAVARLFALNSGSIVDSDKPYAEFWMGTHDSGPSFLIGAAENGGAVESDSTSLKSWVSENPNSLGEKVVEKWGSDLPFLFKVLSVAKALSIQAHPDKELAKILHKLQPKAYRDANHKPEMALAITEFEALCGFISLEELKDVLRSVPETVEMIGHEDTNRVLLLDDEDEEEDLKSVLRSAFTQLMSATKSMISGTISDLKRRLHSESEVRQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECMATSDNVVRAGLTPKFRDVQTLCAMLTYKLGFPEILQGVPVNPYITKYIPPFDEFEVDCCVLPEGASVLFPASPGPSICLVARGEGMMLPLESLESDVISEGDVLFVAAKTEISITSEVELLIFRTGVNSRFFDAFMNGK
Homology
BLAST of MC08g1814 vs. ExPASy Swiss-Prot
Match: Q9M884 (Mannose-6-phosphate isomerase 1 OS=Arabidopsis thaliana OX=3702 GN=PMI1 PE=1 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 4.0e-147
Identity = 260/416 (62.50%), Postives = 322/416 (77.40%), Query Frame = 0

Query: 16  RLKCSVQNYDWGIRGPDSAVARLFALNSGSIVDSDKPYAEFWMGTHDSGPSFLIGAAENG 75
           RL+CSV++YDWG  G DS V R++A NS   +D  +PYAE WMGTH+SGPS+L  A  + 
Sbjct: 22  RLRCSVKDYDWGKIGSDSLVYRVYAANSDYEIDPTRPYAELWMGTHESGPSYLEDADGSN 81

Query: 76  GAVESDSTSLKSWVSENPNSLGEKVVEKWGSDLPFLFKVLSVAKALSIQAHPDKELAKIL 135
           G       +L+SW++ENP SLG +V+EKWG DLPFLFKVLSVA+ LSIQAHPDK+LAK +
Sbjct: 82  G------VTLRSWITENPKSLGNRVLEKWGCDLPFLFKVLSVARPLSIQAHPDKKLAKKM 141

Query: 136 HKLQPKAYRDANHKPEMALAITEFEALCGFISLEELKDVLRSVPETVEMIGHEDTNRVLL 195
           HK  P  Y+D NHKPEMALA T+FEALCGFI L+ELK V+R++PE  E++G E+ N+V  
Sbjct: 142 HKAHPNLYKDDNHKPEMALAYTQFEALCGFIPLQELKSVIRAIPEIEELVGSEEANQVFC 201

Query: 196 LDDEDEEEDLKSVLRSAFTQLMSATKSMISGTISDLKRRLHSESEVRQLTAKELLVLRLE 255
           + + DEE+ +KSV+R+ FT LMSA        +S LKRRLH ES+ RQLT KE LVL+LE
Sbjct: 202 ITEHDEEK-VKSVVRTIFTLLMSADADTTKKIVSKLKRRLHMESQERQLTDKERLVLKLE 261

Query: 256 KQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECMATSDNVVRAGLTPKF 315
           KQYP+D+GVI+AFF NYVKLNPGEALYLGANEPHAY+ GEC+E MATSDNVVRAGLT K 
Sbjct: 262 KQYPNDIGVISAFFFNYVKLNPGEALYLGANEPHAYLFGECLEVMATSDNVVRAGLTSKP 321

Query: 316 RDVQTLCAMLTYKLGFPEILQGVPVNPYITKYIPPFDEFEVDCCVLPEGASVLFPASPGP 375
            D+QTLC+ML+YKLG+PEIL+G  + PYIT+Y+PPF+EFEVD C LP GAS +FP+ PGP
Sbjct: 322 LDIQTLCSMLSYKLGYPEILKGTRIRPYITRYLPPFEEFEVDLCDLPSGASTVFPSVPGP 381

Query: 376 SICLVARGEGMMLPLESLESDVISEGDVLFVAAKTEISITSEVELLIFRTGVNSRF 432
           S+ LV +GEG M    S  +D IS GDVLFV A TEI + S  +L ++R G+NSRF
Sbjct: 382 SLLLVLQGEGRMSTEAS--ADGISMGDVLFVPADTEIHLRSSSDLKLYRAGINSRF 428

BLAST of MC08g1814 vs. ExPASy Swiss-Prot
Match: Q9FZH5 (Mannose-6-phosphate isomerase 2 OS=Arabidopsis thaliana OX=3702 GN=PMI2 PE=1 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 1.7e-142
Identity = 252/425 (59.29%), Postives = 321/425 (75.53%), Query Frame = 0

Query: 9   TKQSNVFRLKCSVQNYDWGIRGPDSAVARLFALNSGSIVDSDKPYAEFWMGTHDSGPSFL 68
           T    + RL+C V+NY+WG  GP+S VARL   N+G  VDS+ PYAEFWMGTH+SGPS  
Sbjct: 17  TGGEEIQRLRCFVKNYEWGKLGPESLVARLQEANTGQRVDSEIPYAEFWMGTHESGPSH- 76

Query: 69  IGAAENGGAVESDSTSLKSWVSENPNSLGEKVVEKWGSDLPFLFKVLSVAKALSIQAHPD 128
           +      G  +    +LKSWV +NPN LG KVV+KWG DLPFLFKVLSV KALSIQAHP+
Sbjct: 77  VEFGSGHGVSDKCMVTLKSWVLDNPNLLGSKVVDKWGCDLPFLFKVLSVTKALSIQAHPN 136

Query: 129 KELAKILHKLQPKAYRDANHKPEMALAITEFEALCGFISLEELKDVLRSVPETVEMIGHE 188
           K LA+ LH+  P  YRD NHKPE+ALA+T F+ALCGF++L+ELK+V+ +VPE  E++G +
Sbjct: 137 KALAEKLHREDPLLYRDNNHKPEIALAVTPFQALCGFVTLKELKEVITNVPEITELVGSK 196

Query: 189 DTNRVLLLDDEDEEEDLKSVLRSAFTQLMSATKSMISGTISDLKRRLHSESEVRQLTAKE 248
             +++  + + DE+E +KSV+R  FTQLMSA+ +     +S +K RL  E++ R+L+ KE
Sbjct: 197 AADQIFNVHEHDEDERIKSVVRLIFTQLMSASNNETKQVVSRMKNRLLLETKHRELSEKE 256

Query: 249 LLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECMATSDNVVR 308
            LVL LEKQY  D+GVI+AFF NYVKLNPGEALYL ANEPHAYISG+C+ECMA SDNVVR
Sbjct: 257 KLVLELEKQYTGDIGVISAFFFNYVKLNPGEALYLDANEPHAYISGDCVECMAASDNVVR 316

Query: 309 AGLTPKFRDVQTLCAMLTYKLGFPEILQGVPVNPYITKYIPPFDEFEVDCCVLPEGASVL 368
           AGLTPK RDVQTLC+MLTYKLG+PEIL+G P+ PY+T+Y+PPFDEFEVD C LP G S +
Sbjct: 317 AGLTPKHRDVQTLCSMLTYKLGYPEILKGFPLTPYVTRYLPPFDEFEVDHCDLPRGKSTV 376

Query: 369 FPASPGPSICLVARGEGMMLPLESLESDVISEGDVLFVAAKTEISITSEVELL-IFRTGV 428
           FPA PGPS+ LV  G+G +    S    +++ GDVLFV A  EI +T E +++ ++R GV
Sbjct: 377 FPAVPGPSVYLVIEGKGQLRTGSS--KVLVNRGDVLFVPADIEIHVTGESDVMKLYRAGV 436

Query: 429 NSRFF 433
           +SRFF
Sbjct: 437 SSRFF 438

BLAST of MC08g1814 vs. ExPASy Swiss-Prot
Match: Q54PA0 (Probable mannose-6-phosphate isomerase OS=Dictyostelium discoideum OX=44689 GN=mpi PE=3 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 1.2e-79
Identity = 188/449 (41.87%), Postives = 261/449 (58.13%), Query Frame = 0

Query: 12  SNVFRLKCSVQNYDWGIRGPDSAVARL---FALNSGSIVDSDKPYAEFWMGTHDSGPSFL 71
           +N+  LKC  QNY+WG  G +S VA+L   +A     I+    PYAE WMG H S PS +
Sbjct: 19  NNIVLLKCVSQNYEWGKYGSNSTVAKLLKGYAKECSDIIKETIPYAELWMGDHVSAPSKV 78

Query: 72  ------IGAAENGGAVESDSTSLKSWVSENPNSLGEKVVEK-WGSDLPFLFKVLSVAKAL 131
                 +   E    V+ +     S  S + +S+  ++VEK +G+D PFLFKVLS+  AL
Sbjct: 79  EYKNKELKLREYIDTVQKEINEKSSTSSSSSSSIRGEIVEKRFGNDFPFLFKVLSIRTAL 138

Query: 132 SIQAHPDKELAKILHKLQPKAYRDANHKPEMALAITEFEALCGFISLEELKDVLRSVPET 191
           SIQAHPD +LA++L K  P  Y+D  HKPE+A+A T FEALC F  L E++  + ++PE 
Sbjct: 139 SIQAHPDSQLAQVLFKKYPNIYKDPYHKPEIAIATTPFEALCSFRPLSEIQSFIDTIPEF 198

Query: 192 VEMIGHEDTNRVLLLDDEDEEEDLKSVLRSAFTQLMSATKSMISGTISDLKRRLHSESEV 251
              +     N ++ LD      D K  L+S  T L+ A   +IS  + +L  RL+ + E 
Sbjct: 199 KNSL----PNNLIKLD------DCKEYLKSIVTSLLKADGLLISNNLKELNNRLNEKRE- 258

Query: 252 RQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECMA 311
            +    + LVL+L  QYP DVGV  A+ LNY+ L PGEAL+LGA EPHAYISG+C+ECMA
Sbjct: 259 EERDDLDRLVLKLYSQYPGDVGVFFAYILNYIVLKPGEALFLGAGEPHAYISGDCVECMA 318

Query: 312 TSDNVVRAGLTPKFRDVQTLCAMLTYKLGFPEILQGVP-VNPYITK-----YIPPFDEFE 371
            SDNVVRAGLTPK +DV TL  MLTY+ G P+++  VP   P ++      Y PP DEF+
Sbjct: 319 PSDNVVRAGLTPKLKDVDTLGDMLTYRTGRPDLV--VPQQRPDLSLTNYRCYQPPVDEFQ 378

Query: 372 V------DCCVLPEGASVLFPASPGPSICLVARGEGMMLPLESLESDV--ISEGDVLFVA 431
           V      DCC      ++   +S GPSI LV  G  + +  +S +S +  +  G +LFV 
Sbjct: 379 VEYYHLDDCC-----KNINVYSSKGPSIVLVYSG-NLSIENKSNQSTLNNLHTGSILFVP 438

Query: 432 AKTE---ISITSEVELLIFRTGVNSRFFD 434
           A T+   I   S + + I+   V++R F+
Sbjct: 439 ANTDYQFIQSDSSIPVSIYVASVSNRIFN 448

BLAST of MC08g1814 vs. ExPASy Swiss-Prot
Match: Q8HXX2 (Mannose-6-phosphate isomerase OS=Macaca fascicularis OX=9541 GN=MPI PE=2 SV=3)

HSP 1 Score: 293.5 bits (750), Expect = 4.0e-78
Identity = 175/423 (41.37%), Postives = 247/423 (58.39%), Query Frame = 0

Query: 14  VFRLKCSVQNYDWGIRGPDSAVARLFALNSG-SIVDSDKPYAEFWMGTHDSGPSFLIGAA 73
           VF L C+VQ Y WG  G +S VARL A +   + +  DKPYAE WMGTH  G + +    
Sbjct: 6   VFPLSCAVQQYAWGKMGYNSEVARLLASSDPLAQIAEDKPYAELWMGTHPRGDAKIF--- 65

Query: 74  ENGGAVESDSTSLKSWVSENPNSLGEKVVEKWGSDLPFLFKVLSVAKALSIQAHPDKELA 133
           +NG        +L  W++EN +SLG KV + +  +LPFLFKVLSV   LSIQAHP+KELA
Sbjct: 66  DNG----ISQKTLGQWIAENQDSLGSKVKDTFNGNLPFLFKVLSVETPLSIQAHPNKELA 125

Query: 134 KILHKLQPKAYRDANHKPEMALAITEFEALCGFISLEELKDVLRSVPETVEMIGHEDTNR 193
           + LH   P+ Y DANHKPEMA+A+T F+ LCGF  +EE+   L+ VPE   +IG E    
Sbjct: 126 EKLHLQAPQHYPDANHKPEMAIALTPFQGLCGFRPVEEIVTFLKKVPEFQFLIGDEAATH 185

Query: 194 VLLLDDEDEEEDLKSVLRSAFTQLMSATKSMISGTISDLKRRLHSE----SEVRQLTAKE 253
           +      D +  + S L+S F+ LM + K +++  ++ L +R+  +    + +  +  + 
Sbjct: 186 LKQTMSHDSQA-VASALQSCFSHLMKSEKKVVAEQLNLLVKRISQQVAAGNNMEDIFGE- 245

Query: 254 LLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECMATSDNVVR 313
            L+L+L +QYP D+G  A +FLN + L PGEA++L AN PHAY+ G+C+ECMA SDN VR
Sbjct: 246 -LLLQLHQQYPGDIGCFAIYFLNLLNLKPGEAMFLEANVPHAYLKGDCVECMACSDNTVR 305

Query: 314 AGLTPKFRDVQTLCAMLTYKLGFPEILQGVPV----NPYITKYIPPFDEFEVDCCVLPEG 373
           AGLTPKF DV TLC ML+Y     +    +P     +PY++ Y PP  +F +    +P  
Sbjct: 306 AGLTPKFIDVPTLCEMLSYTPSSSKDRLFLPTRSQEDPYLSIYDPPVPDFAIMKMEVPGS 365

Query: 374 AS-VLFPASPGPSICLVARGEGMMLPLESLESDVISEGDVLFVAAKTEIS--ITSEVELL 425
            +     A    SI LV +G  +     +     +  G VLF+ A   +S  +T   +LL
Sbjct: 366 VTEYKVLALDSASILLVVQGTVIASTPTTQTPIPLQRGGVLFIGANESVSLKLTEPKDLL 418

BLAST of MC08g1814 vs. ExPASy Swiss-Prot
Match: A5A6K3 (Mannose-6-phosphate isomerase OS=Pan troglodytes OX=9598 GN=MPI PE=2 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 1.2e-77
Identity = 176/423 (41.61%), Postives = 245/423 (57.92%), Query Frame = 0

Query: 14  VFRLKCSVQNYDWGIRGPDSAVARLFALNSG-SIVDSDKPYAEFWMGTHDSGPSFLIGAA 73
           VF L C+VQ Y WG  G +S VARL A +   + +  DKPYAE WMGTH  G + ++   
Sbjct: 6   VFPLSCAVQQYAWGKMGSNSEVARLLASSDPLAQIAEDKPYAELWMGTHPRGDAKIL--- 65

Query: 74  ENGGAVESDSTSLKSWVSENPNSLGEKVVEKWGSDLPFLFKVLSVAKALSIQAHPDKELA 133
           +N         +L  W++EN +SLG KV + +  +LPFLFKVLSV   LSIQAHP+KELA
Sbjct: 66  DN----RISQKTLSQWIAENQDSLGSKVKDTFNGNLPFLFKVLSVETPLSIQAHPNKELA 125

Query: 134 KILHKLQPKAYRDANHKPEMALAITEFEALCGFISLEELKDVLRSVPETVEMIGHEDTNR 193
           + LH   P+ Y DANHKPEMA+A+T F+ LCGF  +EE+   L+ VPE   +IG E    
Sbjct: 126 EKLHLQAPQHYPDANHKPEMAIALTPFQGLCGFRPVEEIVTFLKKVPEFQFLIGDEAATH 185

Query: 194 VLLLDDEDEEEDLKSVLRSAFTQLMSATKSMISGTISDLKRRLHSESEVRQLTAKEL--L 253
           +      D +  + S L+S F+ LM + K ++   ++ L +R+  ++            L
Sbjct: 186 LKQTMSHDSQA-VASSLQSCFSHLMKSEKKVVVEQLNLLVKRISQQAAAGNNMEDIFGEL 245

Query: 254 VLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECMATSDNVVRAG 313
           +L+L +QYP D+G  A +FLN + L PGEA++L AN PHAY+ G+C+ECMA SDN VRAG
Sbjct: 246 LLQLHQQYPGDIGCFAIYFLNLLTLKPGEAMFLEANVPHAYLKGDCVECMACSDNTVRAG 305

Query: 314 LTPKFRDVQTLCAMLTYKLGFPEILQGVPV----NPYITKYIPPFDEFEVDCCVLP---E 373
           LTPKF DV TLC ML+Y     +    +P     +PY++ Y PP  +F +    +P    
Sbjct: 306 LTPKFIDVPTLCEMLSYTPSSSKDRLFLPTRSREDPYLSIYDPPVPDFTIMKTEVPGSVT 365

Query: 374 GASVLFPASPGPSICLVARGEGMMLPLESLESDVISEGDVLFVAAKTEIS--ITSEVELL 425
           G  VL  A    SI L+ +G  +     +     +  G VLF+ A   +S  +T   +LL
Sbjct: 366 GYKVL--ALDSASILLMVQGTVIASTPTTQTPIPLQRGGVLFIGANESVSLKLTEPKDLL 418

BLAST of MC08g1814 vs. NCBI nr
Match: XP_022143258.1 (mannose-6-phosphate isomerase 1 [Momordica charantia])

HSP 1 Score: 865 bits (2234), Expect = 0.0
Identity = 439/439 (100.00%), Postives = 439/439 (100.00%), Query Frame = 0

Query: 1   MESEAVLRTKQSNVFRLKCSVQNYDWGIRGPDSAVARLFALNSGSIVDSDKPYAEFWMGT 60
           MESEAVLRTKQSNVFRLKCSVQNYDWGIRGPDSAVARLFALNSGSIVDSDKPYAEFWMGT
Sbjct: 1   MESEAVLRTKQSNVFRLKCSVQNYDWGIRGPDSAVARLFALNSGSIVDSDKPYAEFWMGT 60

Query: 61  HDSGPSFLIGAAENGGAVESDSTSLKSWVSENPNSLGEKVVEKWGSDLPFLFKVLSVAKA 120
           HDSGPSFLIGAAENGGAVESDSTSLKSWVSENPNSLGEKVVEKWGSDLPFLFKVLSVAKA
Sbjct: 61  HDSGPSFLIGAAENGGAVESDSTSLKSWVSENPNSLGEKVVEKWGSDLPFLFKVLSVAKA 120

Query: 121 LSIQAHPDKELAKILHKLQPKAYRDANHKPEMALAITEFEALCGFISLEELKDVLRSVPE 180
           LSIQAHPDKELAKILHKLQPKAYRDANHKPEMALAITEFEALCGFISLEELKDVLRSVPE
Sbjct: 121 LSIQAHPDKELAKILHKLQPKAYRDANHKPEMALAITEFEALCGFISLEELKDVLRSVPE 180

Query: 181 TVEMIGHEDTNRVLLLDDEDEEEDLKSVLRSAFTQLMSATKSMISGTISDLKRRLHSESE 240
           TVEMIGHEDTNRVLLLDDEDEEEDLKSVLRSAFTQLMSATKSMISGTISDLKRRLHSESE
Sbjct: 181 TVEMIGHEDTNRVLLLDDEDEEEDLKSVLRSAFTQLMSATKSMISGTISDLKRRLHSESE 240

Query: 241 VRQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECM 300
           VRQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECM
Sbjct: 241 VRQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECM 300

Query: 301 ATSDNVVRAGLTPKFRDVQTLCAMLTYKLGFPEILQGVPVNPYITKYIPPFDEFEVDCCV 360
           ATSDNVVRAGLTPKFRDVQTLCAMLTYKLGFPEILQGVPVNPYITKYIPPFDEFEVDCCV
Sbjct: 301 ATSDNVVRAGLTPKFRDVQTLCAMLTYKLGFPEILQGVPVNPYITKYIPPFDEFEVDCCV 360

Query: 361 LPEGASVLFPASPGPSICLVARGEGMMLPLESLESDVISEGDVLFVAAKTEISITSEVEL 420
           LPEGASVLFPASPGPSICLVARGEGMMLPLESLESDVISEGDVLFVAAKTEISITSEVEL
Sbjct: 361 LPEGASVLFPASPGPSICLVARGEGMMLPLESLESDVISEGDVLFVAAKTEISITSEVEL 420

Query: 421 LIFRTGVNSRFFDAFMNGK 439
           LIFRTGVNSRFFDAFMNGK
Sbjct: 421 LIFRTGVNSRFFDAFMNGK 439

BLAST of MC08g1814 vs. NCBI nr
Match: KAG7017923.1 (Mannose-6-phosphate isomerase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 746 bits (1925), Expect = 2.57e-269
Identity = 376/439 (85.65%), Postives = 402/439 (91.57%), Query Frame = 0

Query: 1   MESEAVLRTKQSNVFRLKCSVQNYDWGIRGPDSAVARLFALNSGSIVDSDKPYAEFWMGT 60
           MES+AV RTKQSN+FRLKCSV NYDWGIRG DS VARLFALNSGSI+D DKPYAEFWMGT
Sbjct: 1   MESDAVSRTKQSNIFRLKCSVHNYDWGIRGYDSRVARLFALNSGSIIDPDKPYAEFWMGT 60

Query: 61  HDSGPSFLIGAAENGGAVESDSTSLKSWVSENPNSLGEKVVEKWGSDLPFLFKVLSVAKA 120
           HDSGPSFLI A ENGG+V+SDSTSLKSWVSENPN LG+KVVEKWG+D+PFLFKVLSVAK 
Sbjct: 61  HDSGPSFLIAATENGGSVDSDSTSLKSWVSENPNVLGDKVVEKWGADIPFLFKVLSVAKP 120

Query: 121 LSIQAHPDKELAKILHKLQPKAYRDANHKPEMALAITEFEALCGFISLEELKDVLRSVPE 180
           LSIQAHPDKELAK LH LQPK YRDANHKPEMALAITEFEALCGFISLEELKDVL +VPE
Sbjct: 121 LSIQAHPDKELAKELHMLQPKVYRDANHKPEMALAITEFEALCGFISLEELKDVLHTVPE 180

Query: 181 TVEMIGHEDTNRVLLLDDEDEEEDLKSVLRSAFTQLMSATKSMISGTISDLKRRLHSESE 240
            VEMIG  DTNRVLLL+DED+E+DLKS LRSAFTQLMSA+KS+IS  IS+LKRRL+SESE
Sbjct: 181 IVEMIGDADTNRVLLLNDEDDEDDLKSALRSAFTQLMSASKSLISKAISELKRRLNSESE 240

Query: 241 VRQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECM 300
           VR LTAKE LVL+LEKQYPDDVGVIAAFFLNYVKL PGEAL+LGANEPHAYISGECIECM
Sbjct: 241 VRPLTAKEALVLQLEKQYPDDVGVIAAFFLNYVKLTPGEALHLGANEPHAYISGECIECM 300

Query: 301 ATSDNVVRAGLTPKFRDVQTLCAMLTYKLGFPEILQGVPVNPYITKYIPPFDEFEVDCCV 360
           ATSDNV+RAGLTPKFRDVQTLCAMLTYK GFPEILQGVP+NPYITKY+PPFDEFEVD CV
Sbjct: 301 ATSDNVIRAGLTPKFRDVQTLCAMLTYKQGFPEILQGVPMNPYITKYVPPFDEFEVDRCV 360

Query: 361 LPEGASVLFPASPGPSICLVARGEGMMLPLESLESDVISEGDVLFVAAKTEISITSEVEL 420
           LP GASV+FPASPGPSI LVA GEG ML  ES  SDVISEGDVLFV  + E++ITSEVEL
Sbjct: 361 LPGGASVVFPASPGPSIFLVAGGEGTMLQEESHRSDVISEGDVLFVPDEAEVNITSEVEL 420

Query: 421 LIFRTGVNSRFFDAFMNGK 439
           LIFRTGVN +FFD FMNGK
Sbjct: 421 LIFRTGVNHKFFDTFMNGK 439

BLAST of MC08g1814 vs. NCBI nr
Match: KAG6581183.1 (Mannose-6-phosphate isomerase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 745 bits (1924), Expect = 3.65e-269
Identity = 376/439 (85.65%), Postives = 402/439 (91.57%), Query Frame = 0

Query: 1   MESEAVLRTKQSNVFRLKCSVQNYDWGIRGPDSAVARLFALNSGSIVDSDKPYAEFWMGT 60
           MES+AV RTKQSN+FRLKCSV NYDWGIRG DS VARLFALNSGSI+D DKPYAEFWMGT
Sbjct: 1   MESDAVSRTKQSNIFRLKCSVHNYDWGIRGYDSRVARLFALNSGSIIDPDKPYAEFWMGT 60

Query: 61  HDSGPSFLIGAAENGGAVESDSTSLKSWVSENPNSLGEKVVEKWGSDLPFLFKVLSVAKA 120
           HDSGPSFLI A ENGG+V+SDSTSLKSWVSENPN LG+KVVEKWG+D+PFLFKVLSVAK 
Sbjct: 61  HDSGPSFLIAATENGGSVDSDSTSLKSWVSENPNVLGDKVVEKWGADIPFLFKVLSVAKP 120

Query: 121 LSIQAHPDKELAKILHKLQPKAYRDANHKPEMALAITEFEALCGFISLEELKDVLRSVPE 180
           LSIQAHPDKELAK LH LQPK YRDANHKPEMALAITEFEALCGFISLEELKDVL +VPE
Sbjct: 121 LSIQAHPDKELAKELHMLQPKVYRDANHKPEMALAITEFEALCGFISLEELKDVLHTVPE 180

Query: 181 TVEMIGHEDTNRVLLLDDEDEEEDLKSVLRSAFTQLMSATKSMISGTISDLKRRLHSESE 240
            VEMIG  DTNRVLLL+DED+E+DLKS LRSAFTQLMSA+KS+IS  IS+LKRRL+SESE
Sbjct: 181 IVEMIGDADTNRVLLLNDEDDEDDLKSALRSAFTQLMSASKSLISKAISELKRRLNSESE 240

Query: 241 VRQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECM 300
           VR LTAKE LVL+LEKQYPDDVGVIAAFFLNYVKL PGEAL+LGANEPHAYISGECIECM
Sbjct: 241 VRPLTAKEALVLQLEKQYPDDVGVIAAFFLNYVKLTPGEALHLGANEPHAYISGECIECM 300

Query: 301 ATSDNVVRAGLTPKFRDVQTLCAMLTYKLGFPEILQGVPVNPYITKYIPPFDEFEVDCCV 360
           ATSDNV+RAGLTPKFRDVQTLCAMLTYK GFPEILQGVP+NPYITKY+PPFDEFEVD CV
Sbjct: 301 ATSDNVIRAGLTPKFRDVQTLCAMLTYKQGFPEILQGVPMNPYITKYVPPFDEFEVDRCV 360

Query: 361 LPEGASVLFPASPGPSICLVARGEGMMLPLESLESDVISEGDVLFVAAKTEISITSEVEL 420
           LP GASV+FPASPGPSI LVA GEG ML  ES  SDVISEGDVLFV  + E++ITSEVEL
Sbjct: 361 LPGGASVVFPASPGPSIFLVAGGEGTMLHEESHRSDVISEGDVLFVPDEAEVNITSEVEL 420

Query: 421 LIFRTGVNSRFFDAFMNGK 439
           LIFRTGVN +FFD FMNGK
Sbjct: 421 LIFRTGVNHKFFDTFMNGK 439

BLAST of MC08g1814 vs. NCBI nr
Match: XP_022934077.1 (mannose-6-phosphate isomerase 1-like [Cucurbita moschata])

HSP 1 Score: 744 bits (1922), Expect = 7.37e-269
Identity = 378/439 (86.10%), Postives = 401/439 (91.34%), Query Frame = 0

Query: 1   MESEAVLRTKQSNVFRLKCSVQNYDWGIRGPDSAVARLFALNSGSIVDSDKPYAEFWMGT 60
           MES+AV RTKQSN+FRLKCSV NYDWGIRG DS VARLFALNSGSI+D DKPYAEFWMGT
Sbjct: 1   MESDAVSRTKQSNIFRLKCSVHNYDWGIRGYDSRVARLFALNSGSIIDPDKPYAEFWMGT 60

Query: 61  HDSGPSFLIGAAENGGAVESDSTSLKSWVSENPNSLGEKVVEKWGSDLPFLFKVLSVAKA 120
           HDSGPSFLI A ENGG+V+SDSTSLKSWVSENPN LG KVVEKWG+D+PFLFKVLSVAK 
Sbjct: 61  HDSGPSFLISATENGGSVDSDSTSLKSWVSENPNVLGYKVVEKWGADIPFLFKVLSVAKP 120

Query: 121 LSIQAHPDKELAKILHKLQPKAYRDANHKPEMALAITEFEALCGFISLEELKDVLRSVPE 180
           LSIQAHPDKELAK LH LQPK YRDANHKPEMALAITEFEALCGFISLEELKDVL +VPE
Sbjct: 121 LSIQAHPDKELAKELHMLQPKVYRDANHKPEMALAITEFEALCGFISLEELKDVLHTVPE 180

Query: 181 TVEMIGHEDTNRVLLLDDEDEEEDLKSVLRSAFTQLMSATKSMISGTISDLKRRLHSESE 240
            VEMIG  DTNRVLLL+DED+E+DLKS LRSAFTQLMSA+KS+IS  IS+LKRRL+SESE
Sbjct: 181 IVEMIGDADTNRVLLLNDEDDEDDLKSALRSAFTQLMSASKSLISKAISELKRRLNSESE 240

Query: 241 VRQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECM 300
           VR LTAKE LVL+LEKQYPDDVGVIAAFFLNYVKL PGEAL+LGANEPHAYISGECIECM
Sbjct: 241 VRPLTAKEALVLQLEKQYPDDVGVIAAFFLNYVKLTPGEALHLGANEPHAYISGECIECM 300

Query: 301 ATSDNVVRAGLTPKFRDVQTLCAMLTYKLGFPEILQGVPVNPYITKYIPPFDEFEVDCCV 360
           ATSDNV+RAGLTPKFRDVQTLCAMLTYK GFPEILQGVP+NPYITKYIPPFDEFEVD CV
Sbjct: 301 ATSDNVIRAGLTPKFRDVQTLCAMLTYKQGFPEILQGVPMNPYITKYIPPFDEFEVDRCV 360

Query: 361 LPEGASVLFPASPGPSICLVARGEGMMLPLESLESDVISEGDVLFVAAKTEISITSEVEL 420
           LP GASV+FPASPGPSI LVA GEG ML  ES  SDVISEGDVLFV  + EI+ITSEVEL
Sbjct: 361 LPGGASVVFPASPGPSIFLVAGGEGTMLHEESHRSDVISEGDVLFVPDEAEINITSEVEL 420

Query: 421 LIFRTGVNSRFFDAFMNGK 439
           LIFRTGVN +FFD FMNGK
Sbjct: 421 LIFRTGVNHKFFDTFMNGK 439

BLAST of MC08g1814 vs. NCBI nr
Match: XP_023526350.1 (mannose-6-phosphate isomerase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 739 bits (1908), Expect = 9.99e-267
Identity = 374/439 (85.19%), Postives = 400/439 (91.12%), Query Frame = 0

Query: 1   MESEAVLRTKQSNVFRLKCSVQNYDWGIRGPDSAVARLFALNSGSIVDSDKPYAEFWMGT 60
           MES+AV RTKQSN+FRLKCSV NYDWGI+G DS VARLFALNSGSI+D DKPYAEFWMGT
Sbjct: 1   MESDAVSRTKQSNIFRLKCSVHNYDWGIKGYDSRVARLFALNSGSIIDPDKPYAEFWMGT 60

Query: 61  HDSGPSFLIGAAENGGAVESDSTSLKSWVSENPNSLGEKVVEKWGSDLPFLFKVLSVAKA 120
           HDSGPSFLI A ENGG+V+SDSTSLKSWVSENPN LG+KVVEKWG+D+PFLFKVLSVAK 
Sbjct: 61  HDSGPSFLIAATENGGSVDSDSTSLKSWVSENPNVLGDKVVEKWGADIPFLFKVLSVAKP 120

Query: 121 LSIQAHPDKELAKILHKLQPKAYRDANHKPEMALAITEFEALCGFISLEELKDVLRSVPE 180
           LSIQAHPDKELAK LH LQPK YRDANHKPEMALAITEFEALCGFISLEELKDVL +VPE
Sbjct: 121 LSIQAHPDKELAKELHMLQPKVYRDANHKPEMALAITEFEALCGFISLEELKDVLHTVPE 180

Query: 181 TVEMIGHEDTNRVLLLDDEDEEEDLKSVLRSAFTQLMSATKSMISGTISDLKRRLHSESE 240
            VEMIG  DTNRVLLL+DED+E+DLKS LR AFTQLMSA+KS+IS  IS+LKRRL+SESE
Sbjct: 181 IVEMIGDADTNRVLLLNDEDDEDDLKSALRLAFTQLMSASKSLISKAISELKRRLNSESE 240

Query: 241 VRQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECM 300
           VR LTAKE LVL+LEKQYPDDVGVIAAFFLNYVKL PGEAL+LGANEPHAYISGECIECM
Sbjct: 241 VRPLTAKEALVLQLEKQYPDDVGVIAAFFLNYVKLTPGEALHLGANEPHAYISGECIECM 300

Query: 301 ATSDNVVRAGLTPKFRDVQTLCAMLTYKLGFPEILQGVPVNPYITKYIPPFDEFEVDCCV 360
           ATSDNV+RAGLTPKFRDVQTLCAMLTYK GFPEILQGV +NPYITKY+PPFDEFEVD CV
Sbjct: 301 ATSDNVIRAGLTPKFRDVQTLCAMLTYKQGFPEILQGVSMNPYITKYVPPFDEFEVDRCV 360

Query: 361 LPEGASVLFPASPGPSICLVARGEGMMLPLESLESDVISEGDVLFVAAKTEISITSEVEL 420
           LP GASV+FPASPGPSI LVA GEG ML  ES  SDVISEGDVLFV  + EI+ITSEVEL
Sbjct: 361 LPGGASVVFPASPGPSIFLVAGGEGTMLHEESHRSDVISEGDVLFVPDEAEINITSEVEL 420

Query: 421 LIFRTGVNSRFFDAFMNGK 439
           LIFRTGVN +FFD FMNGK
Sbjct: 421 LIFRTGVNHKFFDTFMNGK 439

BLAST of MC08g1814 vs. ExPASy TrEMBL
Match: A0A6J1CQ99 (Mannose-6-phosphate isomerase OS=Momordica charantia OX=3673 GN=LOC111013172 PE=3 SV=1)

HSP 1 Score: 865 bits (2234), Expect = 0.0
Identity = 439/439 (100.00%), Postives = 439/439 (100.00%), Query Frame = 0

Query: 1   MESEAVLRTKQSNVFRLKCSVQNYDWGIRGPDSAVARLFALNSGSIVDSDKPYAEFWMGT 60
           MESEAVLRTKQSNVFRLKCSVQNYDWGIRGPDSAVARLFALNSGSIVDSDKPYAEFWMGT
Sbjct: 1   MESEAVLRTKQSNVFRLKCSVQNYDWGIRGPDSAVARLFALNSGSIVDSDKPYAEFWMGT 60

Query: 61  HDSGPSFLIGAAENGGAVESDSTSLKSWVSENPNSLGEKVVEKWGSDLPFLFKVLSVAKA 120
           HDSGPSFLIGAAENGGAVESDSTSLKSWVSENPNSLGEKVVEKWGSDLPFLFKVLSVAKA
Sbjct: 61  HDSGPSFLIGAAENGGAVESDSTSLKSWVSENPNSLGEKVVEKWGSDLPFLFKVLSVAKA 120

Query: 121 LSIQAHPDKELAKILHKLQPKAYRDANHKPEMALAITEFEALCGFISLEELKDVLRSVPE 180
           LSIQAHPDKELAKILHKLQPKAYRDANHKPEMALAITEFEALCGFISLEELKDVLRSVPE
Sbjct: 121 LSIQAHPDKELAKILHKLQPKAYRDANHKPEMALAITEFEALCGFISLEELKDVLRSVPE 180

Query: 181 TVEMIGHEDTNRVLLLDDEDEEEDLKSVLRSAFTQLMSATKSMISGTISDLKRRLHSESE 240
           TVEMIGHEDTNRVLLLDDEDEEEDLKSVLRSAFTQLMSATKSMISGTISDLKRRLHSESE
Sbjct: 181 TVEMIGHEDTNRVLLLDDEDEEEDLKSVLRSAFTQLMSATKSMISGTISDLKRRLHSESE 240

Query: 241 VRQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECM 300
           VRQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECM
Sbjct: 241 VRQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECM 300

Query: 301 ATSDNVVRAGLTPKFRDVQTLCAMLTYKLGFPEILQGVPVNPYITKYIPPFDEFEVDCCV 360
           ATSDNVVRAGLTPKFRDVQTLCAMLTYKLGFPEILQGVPVNPYITKYIPPFDEFEVDCCV
Sbjct: 301 ATSDNVVRAGLTPKFRDVQTLCAMLTYKLGFPEILQGVPVNPYITKYIPPFDEFEVDCCV 360

Query: 361 LPEGASVLFPASPGPSICLVARGEGMMLPLESLESDVISEGDVLFVAAKTEISITSEVEL 420
           LPEGASVLFPASPGPSICLVARGEGMMLPLESLESDVISEGDVLFVAAKTEISITSEVEL
Sbjct: 361 LPEGASVLFPASPGPSICLVARGEGMMLPLESLESDVISEGDVLFVAAKTEISITSEVEL 420

Query: 421 LIFRTGVNSRFFDAFMNGK 439
           LIFRTGVNSRFFDAFMNGK
Sbjct: 421 LIFRTGVNSRFFDAFMNGK 439

BLAST of MC08g1814 vs. ExPASy TrEMBL
Match: A0A6J1F1M9 (Mannose-6-phosphate isomerase OS=Cucurbita moschata OX=3662 GN=LOC111441358 PE=3 SV=1)

HSP 1 Score: 744 bits (1922), Expect = 3.57e-269
Identity = 378/439 (86.10%), Postives = 401/439 (91.34%), Query Frame = 0

Query: 1   MESEAVLRTKQSNVFRLKCSVQNYDWGIRGPDSAVARLFALNSGSIVDSDKPYAEFWMGT 60
           MES+AV RTKQSN+FRLKCSV NYDWGIRG DS VARLFALNSGSI+D DKPYAEFWMGT
Sbjct: 1   MESDAVSRTKQSNIFRLKCSVHNYDWGIRGYDSRVARLFALNSGSIIDPDKPYAEFWMGT 60

Query: 61  HDSGPSFLIGAAENGGAVESDSTSLKSWVSENPNSLGEKVVEKWGSDLPFLFKVLSVAKA 120
           HDSGPSFLI A ENGG+V+SDSTSLKSWVSENPN LG KVVEKWG+D+PFLFKVLSVAK 
Sbjct: 61  HDSGPSFLISATENGGSVDSDSTSLKSWVSENPNVLGYKVVEKWGADIPFLFKVLSVAKP 120

Query: 121 LSIQAHPDKELAKILHKLQPKAYRDANHKPEMALAITEFEALCGFISLEELKDVLRSVPE 180
           LSIQAHPDKELAK LH LQPK YRDANHKPEMALAITEFEALCGFISLEELKDVL +VPE
Sbjct: 121 LSIQAHPDKELAKELHMLQPKVYRDANHKPEMALAITEFEALCGFISLEELKDVLHTVPE 180

Query: 181 TVEMIGHEDTNRVLLLDDEDEEEDLKSVLRSAFTQLMSATKSMISGTISDLKRRLHSESE 240
            VEMIG  DTNRVLLL+DED+E+DLKS LRSAFTQLMSA+KS+IS  IS+LKRRL+SESE
Sbjct: 181 IVEMIGDADTNRVLLLNDEDDEDDLKSALRSAFTQLMSASKSLISKAISELKRRLNSESE 240

Query: 241 VRQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECM 300
           VR LTAKE LVL+LEKQYPDDVGVIAAFFLNYVKL PGEAL+LGANEPHAYISGECIECM
Sbjct: 241 VRPLTAKEALVLQLEKQYPDDVGVIAAFFLNYVKLTPGEALHLGANEPHAYISGECIECM 300

Query: 301 ATSDNVVRAGLTPKFRDVQTLCAMLTYKLGFPEILQGVPVNPYITKYIPPFDEFEVDCCV 360
           ATSDNV+RAGLTPKFRDVQTLCAMLTYK GFPEILQGVP+NPYITKYIPPFDEFEVD CV
Sbjct: 301 ATSDNVIRAGLTPKFRDVQTLCAMLTYKQGFPEILQGVPMNPYITKYIPPFDEFEVDRCV 360

Query: 361 LPEGASVLFPASPGPSICLVARGEGMMLPLESLESDVISEGDVLFVAAKTEISITSEVEL 420
           LP GASV+FPASPGPSI LVA GEG ML  ES  SDVISEGDVLFV  + EI+ITSEVEL
Sbjct: 361 LPGGASVVFPASPGPSIFLVAGGEGTMLHEESHRSDVISEGDVLFVPDEAEINITSEVEL 420

Query: 421 LIFRTGVNSRFFDAFMNGK 439
           LIFRTGVN +FFD FMNGK
Sbjct: 421 LIFRTGVNHKFFDTFMNGK 439

BLAST of MC08g1814 vs. ExPASy TrEMBL
Match: A0A6J1J7Z8 (Mannose-6-phosphate isomerase OS=Cucurbita maxima OX=3661 GN=LOC111482189 PE=3 SV=1)

HSP 1 Score: 739 bits (1908), Expect = 4.84e-267
Identity = 373/439 (84.97%), Postives = 401/439 (91.34%), Query Frame = 0

Query: 1   MESEAVLRTKQSNVFRLKCSVQNYDWGIRGPDSAVARLFALNSGSIVDSDKPYAEFWMGT 60
           MES+AV R KQSN+FRLKCSV+NYDWGIRG DS VARLFALNSGSI+D DKPYAEFWMGT
Sbjct: 1   MESDAVSRMKQSNIFRLKCSVRNYDWGIRGYDSRVARLFALNSGSIIDPDKPYAEFWMGT 60

Query: 61  HDSGPSFLIGAAENGGAVESDSTSLKSWVSENPNSLGEKVVEKWGSDLPFLFKVLSVAKA 120
           HDSGPSFLI A ENGG+V+SDSTSLKSWV ENPN LG KVVEKWG+D+PFLFKVLSVAK 
Sbjct: 61  HDSGPSFLIAATENGGSVDSDSTSLKSWVLENPNVLGYKVVEKWGADIPFLFKVLSVAKP 120

Query: 121 LSIQAHPDKELAKILHKLQPKAYRDANHKPEMALAITEFEALCGFISLEELKDVLRSVPE 180
           LSIQAHPDKELAK LH LQPK YRDANHKPEMALAITEFEALCGFISLEELKDVLR+VPE
Sbjct: 121 LSIQAHPDKELAKELHMLQPKVYRDANHKPEMALAITEFEALCGFISLEELKDVLRTVPE 180

Query: 181 TVEMIGHEDTNRVLLLDDEDEEEDLKSVLRSAFTQLMSATKSMISGTISDLKRRLHSESE 240
            VEMIG  DTNRVLLL+DED+E++LKS LRSAFTQLMSA+KS+IS  IS+LKRRL+SESE
Sbjct: 181 IVEMIGDADTNRVLLLNDEDDEDNLKSALRSAFTQLMSASKSLISKAISELKRRLNSESE 240

Query: 241 VRQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECM 300
           VR LTAKE LVL+LEKQYPDDVGVIAAFFLNYVKL PGEAL+LGANEPHAYISGECIECM
Sbjct: 241 VRPLTAKEALVLQLEKQYPDDVGVIAAFFLNYVKLTPGEALHLGANEPHAYISGECIECM 300

Query: 301 ATSDNVVRAGLTPKFRDVQTLCAMLTYKLGFPEILQGVPVNPYITKYIPPFDEFEVDCCV 360
           ATSDNV+RAGLTPKFRDVQTLCAMLTYK GFPEILQGVP+NPYITKY+PPFDEFEVD CV
Sbjct: 301 ATSDNVIRAGLTPKFRDVQTLCAMLTYKQGFPEILQGVPMNPYITKYVPPFDEFEVDRCV 360

Query: 361 LPEGASVLFPASPGPSICLVARGEGMMLPLESLESDVISEGDVLFVAAKTEISITSEVEL 420
           LP GASV+FPASPGPSI LVA GEG ML  ES  SDVISEGDVLF+  + E++ITSEVEL
Sbjct: 361 LPGGASVVFPASPGPSIFLVAGGEGTMLHEESHRSDVISEGDVLFIPDEAEVNITSEVEL 420

Query: 421 LIFRTGVNSRFFDAFMNGK 439
           LIFRTGVN +FFD FMNGK
Sbjct: 421 LIFRTGVNHKFFDTFMNGK 439

BLAST of MC08g1814 vs. ExPASy TrEMBL
Match: A0A5D3BQZ9 (Mannose-6-phosphate isomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold451G002180 PE=3 SV=1)

HSP 1 Score: 720 bits (1858), Expect = 2.14e-259
Identity = 369/441 (83.67%), Postives = 396/441 (89.80%), Query Frame = 0

Query: 1   MESEAVLRTKQSNVFRLKCSVQNYDWGIRGPDSAVARLFALNSGSIVDSDKPYAEFWMGT 60
           MES+A    KQSNVFRLKCSVQ YDWGIRG DS VARLFALNSGS++D DKPYAEFWMGT
Sbjct: 1   MESDAFPTKKQSNVFRLKCSVQTYDWGIRGQDSLVARLFALNSGSVIDPDKPYAEFWMGT 60

Query: 61  HDSGPSFLIGAA-ENGGAVESDSTSLKSWVSENPNSLGEKVVEKWGSDLPFLFKVLSVAK 120
           HDSGPSFLI AA ENGG ++S STSLKSWV ENPN LG+KVV+KWGSD+PFLFKVLSVAK
Sbjct: 61  HDSGPSFLIPAAVENGGRIDSYSTSLKSWVLENPNVLGDKVVQKWGSDIPFLFKVLSVAK 120

Query: 121 ALSIQAHPDKELAKILHKLQPKAYRDANHKPEMALAITEFEALCGFISLEELKDVLRSVP 180
            LSIQAHPDKELAK LH LQPK YRDANHKPEMALAIT+FEALCGFISLEELK VL +VP
Sbjct: 121 PLSIQAHPDKELAKELHILQPKVYRDANHKPEMALAITDFEALCGFISLEELKYVLHTVP 180

Query: 181 ETVEMIGHEDTNRVLLLDDEDEEEDLKSVLRSAFTQLMSATKSMISGTISDLKRRLHSES 240
           E+VEMIG   TNR+LLLDDEDEEEDLKS LR AFTQLMSA K +IS  IS+L RRLHSES
Sbjct: 181 ESVEMIGTAATNRLLLLDDEDEEEDLKSALRLAFTQLMSAPKLLISKVISELTRRLHSES 240

Query: 241 EVRQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIEC 300
           EVRQLTAKE LVL LEKQYPDDVG+IAAFFLNYVKL+ GEALYLGANEPHAYI GECIEC
Sbjct: 241 EVRQLTAKEALVLNLEKQYPDDVGIIAAFFLNYVKLSAGEALYLGANEPHAYIKGECIEC 300

Query: 301 MATSDNVVRAGLTPKFRDVQTLCAMLTYKLGFPEILQGVPVNPYITKYIPPFDEFEVDCC 360
           MATSDNVVRAGLTPKFRDVQTLCAMLTYK GFPEILQGVP+NPYITKY+PPFDEFEVD C
Sbjct: 301 MATSDNVVRAGLTPKFRDVQTLCAMLTYKQGFPEILQGVPMNPYITKYVPPFDEFEVDRC 360

Query: 361 VLPEGASVLFPASPGPSICLVARGEGMM-LPLESLESDVISEGDVLFVAAKTEISITSEV 420
            LP+G SVLFP+SPGPSI LVA G+GMM L  ES+ESDVISEGDVLFV A+TEI++TSEV
Sbjct: 361 ALPKGTSVLFPSSPGPSIFLVAGGKGMMMLNEESVESDVISEGDVLFVPAETEINLTSEV 420

Query: 421 ELLIFRTGVNSRFFDAFMNGK 439
           ELLIFRTGVN++FFD FMNGK
Sbjct: 421 ELLIFRTGVNNKFFDTFMNGK 441

BLAST of MC08g1814 vs. ExPASy TrEMBL
Match: A0A0A0L9Y1 (Mannose-6-phosphate isomerase OS=Cucumis sativus OX=3659 GN=Csa_3G300600 PE=3 SV=1)

HSP 1 Score: 720 bits (1858), Expect = 2.14e-259
Identity = 369/441 (83.67%), Postives = 396/441 (89.80%), Query Frame = 0

Query: 1   MESEAVLRTKQSNVFRLKCSVQNYDWGIRGPDSAVARLFALNSGSIVDSDKPYAEFWMGT 60
           MES+A+   KQSNVF+LKCSVQ YDWGIRG DS VARLFALNSGS++D DKPYAEFWMGT
Sbjct: 1   MESDALPTKKQSNVFQLKCSVQTYDWGIRGQDSLVARLFALNSGSLIDPDKPYAEFWMGT 60

Query: 61  HDSGPSFLIG-AAENGGAVESDSTSLKSWVSENPNSLGEKVVEKWGSDLPFLFKVLSVAK 120
           HDSGPSFLI  A ENGG ++S STSLKSWV ENPN LG+KVV+KWGSD+PFLFKVLSVAK
Sbjct: 61  HDSGPSFLIPPAVENGGRIDSYSTSLKSWVLENPNVLGDKVVQKWGSDIPFLFKVLSVAK 120

Query: 121 ALSIQAHPDKELAKILHKLQPKAYRDANHKPEMALAITEFEALCGFISLEELKDVLRSVP 180
            LSIQAHPDKELAK LH L PK YRDANHKPEMALAIT+FEALCGFISLEELKDVL +VP
Sbjct: 121 PLSIQAHPDKELAKELHILHPKVYRDANHKPEMALAITDFEALCGFISLEELKDVLHTVP 180

Query: 181 ETVEMIGHEDTNRVLLLDDEDEEEDLKSVLRSAFTQLMSATKSMISGTISDLKRRLHSES 240
           E+VEMIG   TNR+LLLDDEDEEEDLKS LR AFT+LMSA   +IS  IS+L RRLHSES
Sbjct: 181 ESVEMIGTAATNRLLLLDDEDEEEDLKSALRLAFTRLMSAPTLLISKVISELTRRLHSES 240

Query: 241 EVRQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIEC 300
           EVRQLTAKE+LVL LEKQYPDDVGVIAAFFLNYVKL+PGEALYLGANEPHAYI GECIEC
Sbjct: 241 EVRQLTAKEVLVLNLEKQYPDDVGVIAAFFLNYVKLSPGEALYLGANEPHAYIKGECIEC 300

Query: 301 MATSDNVVRAGLTPKFRDVQTLCAMLTYKLGFPEILQGVPVNPYITKYIPPFDEFEVDCC 360
           MATSDNVVRAGLTPKFRDVQTLCAMLTYK GFPEILQGVPVNPYITKY+PPFDEFEVD C
Sbjct: 301 MATSDNVVRAGLTPKFRDVQTLCAMLTYKQGFPEILQGVPVNPYITKYVPPFDEFEVDRC 360

Query: 361 VLPEGASVLFPASPGPSICLVARGEGMML-PLESLESDVISEGDVLFVAAKTEISITSEV 420
            LP G SVLFPASPGPSI LVA G+GMML   ESL+SDVISEGDVLFV A+TEI++TSEV
Sbjct: 361 ALPMGTSVLFPASPGPSIFLVAGGKGMMLLNEESLKSDVISEGDVLFVPAETEINLTSEV 420

Query: 421 ELLIFRTGVNSRFFDAFMNGK 439
           ELLIFRTGVN++FFD FMNGK
Sbjct: 421 ELLIFRTGVNNKFFDTFMNGK 441

BLAST of MC08g1814 vs. TAIR 10
Match: AT3G02570.1 (Mannose-6-phosphate isomerase, type I )

HSP 1 Score: 522.7 bits (1345), Expect = 2.8e-148
Identity = 260/416 (62.50%), Postives = 322/416 (77.40%), Query Frame = 0

Query: 16  RLKCSVQNYDWGIRGPDSAVARLFALNSGSIVDSDKPYAEFWMGTHDSGPSFLIGAAENG 75
           RL+CSV++YDWG  G DS V R++A NS   +D  +PYAE WMGTH+SGPS+L  A  + 
Sbjct: 22  RLRCSVKDYDWGKIGSDSLVYRVYAANSDYEIDPTRPYAELWMGTHESGPSYLEDADGSN 81

Query: 76  GAVESDSTSLKSWVSENPNSLGEKVVEKWGSDLPFLFKVLSVAKALSIQAHPDKELAKIL 135
           G       +L+SW++ENP SLG +V+EKWG DLPFLFKVLSVA+ LSIQAHPDK+LAK +
Sbjct: 82  G------VTLRSWITENPKSLGNRVLEKWGCDLPFLFKVLSVARPLSIQAHPDKKLAKKM 141

Query: 136 HKLQPKAYRDANHKPEMALAITEFEALCGFISLEELKDVLRSVPETVEMIGHEDTNRVLL 195
           HK  P  Y+D NHKPEMALA T+FEALCGFI L+ELK V+R++PE  E++G E+ N+V  
Sbjct: 142 HKAHPNLYKDDNHKPEMALAYTQFEALCGFIPLQELKSVIRAIPEIEELVGSEEANQVFC 201

Query: 196 LDDEDEEEDLKSVLRSAFTQLMSATKSMISGTISDLKRRLHSESEVRQLTAKELLVLRLE 255
           + + DEE+ +KSV+R+ FT LMSA        +S LKRRLH ES+ RQLT KE LVL+LE
Sbjct: 202 ITEHDEEK-VKSVVRTIFTLLMSADADTTKKIVSKLKRRLHMESQERQLTDKERLVLKLE 261

Query: 256 KQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECMATSDNVVRAGLTPKF 315
           KQYP+D+GVI+AFF NYVKLNPGEALYLGANEPHAY+ GEC+E MATSDNVVRAGLT K 
Sbjct: 262 KQYPNDIGVISAFFFNYVKLNPGEALYLGANEPHAYLFGECLEVMATSDNVVRAGLTSKP 321

Query: 316 RDVQTLCAMLTYKLGFPEILQGVPVNPYITKYIPPFDEFEVDCCVLPEGASVLFPASPGP 375
            D+QTLC+ML+YKLG+PEIL+G  + PYIT+Y+PPF+EFEVD C LP GAS +FP+ PGP
Sbjct: 322 LDIQTLCSMLSYKLGYPEILKGTRIRPYITRYLPPFEEFEVDLCDLPSGASTVFPSVPGP 381

Query: 376 SICLVARGEGMMLPLESLESDVISEGDVLFVAAKTEISITSEVELLIFRTGVNSRF 432
           S+ LV +GEG M    S  +D IS GDVLFV A TEI + S  +L ++R G+NSRF
Sbjct: 382 SLLLVLQGEGRMSTEAS--ADGISMGDVLFVPADTEIHLRSSSDLKLYRAGINSRF 428

BLAST of MC08g1814 vs. TAIR 10
Match: AT1G67070.1 (Mannose-6-phosphate isomerase, type I )

HSP 1 Score: 507.3 bits (1305), Expect = 1.2e-143
Identity = 252/425 (59.29%), Postives = 321/425 (75.53%), Query Frame = 0

Query: 9   TKQSNVFRLKCSVQNYDWGIRGPDSAVARLFALNSGSIVDSDKPYAEFWMGTHDSGPSFL 68
           T    + RL+C V+NY+WG  GP+S VARL   N+G  VDS+ PYAEFWMGTH+SGPS  
Sbjct: 17  TGGEEIQRLRCFVKNYEWGKLGPESLVARLQEANTGQRVDSEIPYAEFWMGTHESGPSH- 76

Query: 69  IGAAENGGAVESDSTSLKSWVSENPNSLGEKVVEKWGSDLPFLFKVLSVAKALSIQAHPD 128
           +      G  +    +LKSWV +NPN LG KVV+KWG DLPFLFKVLSV KALSIQAHP+
Sbjct: 77  VEFGSGHGVSDKCMVTLKSWVLDNPNLLGSKVVDKWGCDLPFLFKVLSVTKALSIQAHPN 136

Query: 129 KELAKILHKLQPKAYRDANHKPEMALAITEFEALCGFISLEELKDVLRSVPETVEMIGHE 188
           K LA+ LH+  P  YRD NHKPE+ALA+T F+ALCGF++L+ELK+V+ +VPE  E++G +
Sbjct: 137 KALAEKLHREDPLLYRDNNHKPEIALAVTPFQALCGFVTLKELKEVITNVPEITELVGSK 196

Query: 189 DTNRVLLLDDEDEEEDLKSVLRSAFTQLMSATKSMISGTISDLKRRLHSESEVRQLTAKE 248
             +++  + + DE+E +KSV+R  FTQLMSA+ +     +S +K RL  E++ R+L+ KE
Sbjct: 197 AADQIFNVHEHDEDERIKSVVRLIFTQLMSASNNETKQVVSRMKNRLLLETKHRELSEKE 256

Query: 249 LLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIECMATSDNVVR 308
            LVL LEKQY  D+GVI+AFF NYVKLNPGEALYL ANEPHAYISG+C+ECMA SDNVVR
Sbjct: 257 KLVLELEKQYTGDIGVISAFFFNYVKLNPGEALYLDANEPHAYISGDCVECMAASDNVVR 316

Query: 309 AGLTPKFRDVQTLCAMLTYKLGFPEILQGVPVNPYITKYIPPFDEFEVDCCVLPEGASVL 368
           AGLTPK RDVQTLC+MLTYKLG+PEIL+G P+ PY+T+Y+PPFDEFEVD C LP G S +
Sbjct: 317 AGLTPKHRDVQTLCSMLTYKLGYPEILKGFPLTPYVTRYLPPFDEFEVDHCDLPRGKSTV 376

Query: 369 FPASPGPSICLVARGEGMMLPLESLESDVISEGDVLFVAAKTEISITSEVELL-IFRTGV 428
           FPA PGPS+ LV  G+G +    S    +++ GDVLFV A  EI +T E +++ ++R GV
Sbjct: 377 FPAVPGPSVYLVIEGKGQLRTGSS--KVLVNRGDVLFVPADIEIHVTGESDVMKLYRAGV 436

Query: 429 NSRFF 433
           +SRFF
Sbjct: 437 SSRFF 438

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M8844.0e-14762.50Mannose-6-phosphate isomerase 1 OS=Arabidopsis thaliana OX=3702 GN=PMI1 PE=1 SV=... [more]
Q9FZH51.7e-14259.29Mannose-6-phosphate isomerase 2 OS=Arabidopsis thaliana OX=3702 GN=PMI2 PE=1 SV=... [more]
Q54PA01.2e-7941.87Probable mannose-6-phosphate isomerase OS=Dictyostelium discoideum OX=44689 GN=m... [more]
Q8HXX24.0e-7841.37Mannose-6-phosphate isomerase OS=Macaca fascicularis OX=9541 GN=MPI PE=2 SV=3[more]
A5A6K31.2e-7741.61Mannose-6-phosphate isomerase OS=Pan troglodytes OX=9598 GN=MPI PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022143258.10.0100.00mannose-6-phosphate isomerase 1 [Momordica charantia][more]
KAG7017923.12.57e-26985.65Mannose-6-phosphate isomerase 1 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6581183.13.65e-26985.65Mannose-6-phosphate isomerase 1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022934077.17.37e-26986.10mannose-6-phosphate isomerase 1-like [Cucurbita moschata][more]
XP_023526350.19.99e-26785.19mannose-6-phosphate isomerase 1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1CQ990.0100.00Mannose-6-phosphate isomerase OS=Momordica charantia OX=3673 GN=LOC111013172 PE=... [more]
A0A6J1F1M93.57e-26986.10Mannose-6-phosphate isomerase OS=Cucurbita moschata OX=3662 GN=LOC111441358 PE=3... [more]
A0A6J1J7Z84.84e-26784.97Mannose-6-phosphate isomerase OS=Cucurbita maxima OX=3661 GN=LOC111482189 PE=3 S... [more]
A0A5D3BQZ92.14e-25983.67Mannose-6-phosphate isomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A0A0L9Y12.14e-25983.67Mannose-6-phosphate isomerase OS=Cucumis sativus OX=3659 GN=Csa_3G300600 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT3G02570.12.8e-14862.50Mannose-6-phosphate isomerase, type I [more]
AT1G67070.11.2e-14359.29Mannose-6-phosphate isomerase, type I [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016305Mannose-6-phosphate isomerasePRINTSPR00714MAN6PISMRASEcoord: 17..35
score: 48.42
coord: 308..327
score: 62.0
coord: 143..166
score: 64.17
coord: 108..129
score: 76.36
coord: 270..289
score: 59.5
coord: 254..269
score: 41.25
coord: 289..308
score: 70.0
IPR016305Mannose-6-phosphate isomerasePIRSFPIRSF001480PMIcoord: 11..432
e-value: 2.4E-128
score: 426.8
IPR016305Mannose-6-phosphate isomerasePANTHERPTHR10309MANNOSE-6-PHOSPHATE ISOMERASEcoord: 7..433
IPR001250Mannose-6-phosphate isomerase, type ITIGRFAMTIGR00218TIGR00218coord: 12..422
e-value: 4.2E-78
score: 261.0
IPR001250Mannose-6-phosphate isomerase, type IPFAMPF01238PMI_typeIcoord: 14..386
e-value: 6.2E-111
score: 371.2
NoneNo IPR availableGENE3D1.10.441.10Phosphomannose Isomerase, domain 2coord: 167..333
e-value: 4.9E-137
score: 459.5
NoneNo IPR availablePIRSRPIRSR001480-1PIRSR001480-1coord: 22..425
e-value: 1.7E-124
score: 414.0
NoneNo IPR availablePIRSRPIRSR001480-2PIRSR001480-2coord: 22..425
e-value: 1.7E-124
score: 414.0
NoneNo IPR availablePANTHERPTHR10309:SF10MANNOSE-6-PHOSPHATE ISOMERASEcoord: 7..433
NoneNo IPR availableCDDcd07011cupin_PMI_type_I_Ncoord: 17..327
e-value: 2.26792E-124
score: 359.171
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 18..309
e-value: 4.9E-137
score: 459.5
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 14..424
e-value: 4.9E-137
score: 459.5
IPR018050Phosphomannose isomerase, type I, conserved sitePROSITEPS00965PMI_I_1coord: 143..151
IPR018050Phosphomannose isomerase, type I, conserved sitePROSITEPS00966PMI_I_2coord: 289..314
IPR011051RmlC-like cupin domain superfamilySUPERFAMILY51182RmlC-like cupinscoord: 14..417

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g1814.1MC08g1814.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0000032 cell wall mannoprotein biosynthetic process
biological_process GO:0009298 GDP-mannose biosynthetic process
biological_process GO:0006486 protein glycosylation
cellular_component GO:0005829 cytosol
molecular_function GO:0004476 mannose-6-phosphate isomerase activity
molecular_function GO:0008270 zinc ion binding