MC08g1553 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g1553
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionprotein NRT1/ PTR FAMILY 4.6-like
LocationMC08: 22225178 .. 22229962 (-)
RNA-Seq ExpressionMC08g1553
SyntenyMC08g1553
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGGAGAGCGGGAGGAGATGGGAGGGGTACGTGGACTGGCGGAAGAGGGTGGCGGTGAGGGGGCGGCACGGGGGGATGGCGGCGGCGGGGTTCGTGCTGGGGGTGGAGATACTGGAGAACTTGGCGTTTCTGGCAAATGCAAGCAACTTGGTGATGTACTTGAGGAAGTACATGGGGTTTTCGCCGGAGAAATCGGCAAACCATGTCACGACTTTCATGGCCACAGCCTTCCTTCTCGCCCTTCTTGGTGGCTTCTTATCGGATGCCTTTTTCACCACTTATCACGTCTTCCTTTTCTCCTCCCTCATTGAGTTTCTGGTACTTTTCTATCCCTTTCTCTCTCTCTCAACCAAAGTACATGCATAGAGAACTTAAAGAGTTTTCAACTTTTCTCTCTTTTAATCTTTTCCATTTTGAAAACAAATTATACATGTTTGTGTGTATGATTTTGCTTTACGATGGGTCATGAGATTTGTGGCAACAATTTGAGTTTCTTCTCAGTTTTCTCAAAATTGGGTCATCGTGGGTTTAGAAATACGACACTGAAATTTAGTACAGAGACTAATTGTCTCCTTATTTCTCTCTTAATTGTCAAACATTTATTAATGATAATGACAAAATTAAAGAAATCGGGTACTTTCATTTAATATTAGTCTTTCTTCTACTTTTAAGATACTAGTCTTTCTTCTACTTTTAAGTAGGTCAACACTCATTTTTTATCGACCGCCGTTCAATTTTTGATAGATGGGAGAGTGGAATGAGGAAAAAAAGATATTTTTTATTATTTAAGAAAAATAAATATTTCTCAAATTTTTAGTGGCAATCTTTGAAGATTGCTTTATCGATCAGCACCCATGTAATTGGTGTTAGCTACTTAAAAAAATTTCACAGATTTTTTATTTTTTGAAAAATACAAAAAAATTATATCTGTTTACCTATGTCGAAAATTTAGTAGTGGTCAATACAAGATAAGTGTTGAGCGAGAAATACAAGTTTTTCATTTAAAGTGGTCGAGTGAGAGCAGAGAGGATAAAGAAAGATTGATTTTTTTTTTAATTATTATTATTATTATTTTTGACAAAGGATTTTTTTATTATTATTTTATATTTTGGGGGGTTGAACAATGAAATGAATTGAATTAAATTCAAAGGGGAGATGTCCCACACTCAAAAGAACGTAGGCATCCATCCGTTTCTTTTTCAAGATAATGACACAGATCCCAACAACGAAACTTGTAATTTATAACAAAGAAAAAAAAAGTTAAGTTATCATTTTCATTTTTTAATTTATATGATCTGTGTCTATTTTCAATTTTATTTTAGTAGTTTTAAATTTTCTAACTTCATTCTTTCGTAGCCCTCGGGTTAGTCTTTAAAAATGTTTGTGTTATTACTGATGTTAGGATACAATAGAGAGAGCCTAATGACTTAAAAAAATGTCATTTCAAATTTTTTTTAATATTGATTTAGTAATAAGATTGTTGCTTTAGATATTTAATCAATGTATATGAATTAAATTAAATTAAAATATTAATTATAAAGGCTAAAATGATTAATTGATCTAGAAAAAAGAAAAAACCAGCTAAGAAAATTTTGAATATAATAAAAAGGAGGGTGCATGTATACAGCAATATGGAATTAAAAGACTAATTATTCTTAAGGATAGCTAATTTTTGTTGGATAAGAATACTAGTAAAATCTTTATTTAATATAACAAAAAACTATAGAAATAACCCACAATAAGAAAATATTACATGGAATTCCATTTTTAAAAAATAGAAATTCACCATAAAAGGAAACTAATTAAGTTGGAATAATGACCATAATAGGAATCCAAGTTGGGGACAAAACGAGCCTTTATGAGCCTACACATTGGGAGAAAATTAAAAAAATTAATGTTCGTCAAATTTTGTGGTCAAATGATATATTTATTGTGTTTTTTAGGTGTTAATTTTTAGTTAAATATAAATCAAAATATTTGATAAATAATCACTTTCATAATTAGTTTTATGATCTTAAAACTTCTTTCTTAGTTAAATATTTAAATAAAAGTACTTTATAAATATTTTAAGTTTATGTTATTTTCAAGTATCATATTTTTTAAAGAAGAAGATTTTTGGGCATGAAAATAGTATTTTCGACCTTATCCTTAGATATTCTATTAGAAGTTTCAGTTTCATTTCTAATGCCATGAAGATTTTTAATCAAATTTTTACTCAACAATATCATTAAAATTGAATAATGAATTAGAGAGAATATTTGAAATATGACACGATGAGATGATAAGAAAATATTTCAAATTTAAAAAAGATCCACTCTTATAAAGTAAAATTTTTACATATCATGTATCAACGTTCCATATTTAACTTAAGTGACATTATGAGGTAGTTATAATTATTTTTTTTATCTTTTTTAATTATAAGAGACCGTTAATTATTTTAAAAGAAAATGTATGGCGTTGGCGTGTTATTTTAGAAGGAGAAAAATGATAAATAATTAGGTAGGAAAGAAAAAACAAAAAGAAAAAAAAACAAAAAAAACTTTCCTCTTGGTTTCTTTTTGTTAAAGTGTGGGCCCCACATTGGCGTGAATGCAATGTCATCCACGAGTGTGTGCGTTACGACATTGGAACTCGTCCCTTTGCGTCAAACCAAATAAAGTTTTTTTCTTGTCTTTTTCTTCTTTCTTTTCTTTTCTTTTCTTTTTTTTTTCCCTCAACTTCATATCTTCACTTTTACATTTTTCACTTCTACTGAAAAAATAAAAAACAATATCATTATATGGATGCTGATCGATATCTTTTCACTGATCATCACTATACTTTTTTTTTCCGATTACAAGAAAGGACATGCAAGCAAAGAATAGAGGAGATAGTTTTTTTTTGGAAGACCAAATCTTGTCTTCACTGTCTTGGAAAAATTGACAATTGGTTAATTATTTTAGAAAATCTATGTTTCTTCATTTCTCGCTCTTAATTATCAAACTTTTAATTGTGGTGAGGAAAATGTTACTCCGAGTATTTTCATTTTTTTTTATGCTGATTATTGGAGTTGGAAAAAAAGGGGTTGGTGATCTTGACAGTACAAGCGAAGGTCCCATCTCTAAAACCGGTGCCGTGCAAGGCGACGAATGGGAGCTCATGTAGGGAGGTGGGTGGGGGAGAGGCAGCGATGCTGTTCGTGGGGCTATACCTGGTGGCATTGGGGGTGGGCGGGATAAAGGGATCATTGCCGGCGCACGGGGCGGAGCAGTTCGACGAAGGCACGCCTCAAGGGCGTAGGAGGAGATCCACTTTCTTCAACTACTTCGTCTTCTGTCTCTCTTGTGGGGCTCTTGTGGCCGTCACTCTTGTGGTTTGGATCGAAGACAACTTGGGATGGGAGTGGGGCTTTGGAATCTCAACCATCACCATATTTCTCTCTATTCCTTTATTCCTTTCTGGATCCACCTTCTACCGAAACAAGATACCAACTGGAAGCCCCCTTACTACAATTCTAAAGGTAAATTAGAGTTTAAGAGATACATTTTTCTAGCTGACTGATACTAAAAATTTAATAGTTGTCAGTAACAGATGAATTCTGACTAAATTATACTGTTTGAGTTTGTAGCTATAATATATGTTTGTGTGTGACCAGGTTTTGGTGGCTGCAACTTTGAACCGTTGCTGCAGCAACAAATCATCGACCAACGCAGTGGCGAGCATGAGCACAAGCCCTTCCGTAACAACAACCCTCGAGTTGGAGGAAGGCCAATCCAATAATAATAACCCTGCCGAAACAATGAGTACAGCTCTAACTCCAACAAAAAGCCTAAAATTTCTCAACACAGCAGTAGAAAAACAGCCATTCCACCCATCCCTAAACTGCACAAACCAACAACTAGAAGAAGTGAAAACGGTTCTCAAAGTGCTCCCAATCTTTGCTTGCACCGTCATGCTCAACTGCTGCCTCGCCCAACTCTCGACCTTCTCCGTCCAACAAGCCTCCACAATGGACACCCGAATCGGCTCCCTCAAGCTCCCCCCAGCCTCACTCCCCGTCTTTCCCGTCCTCTTCATCATTGTCCTCGTCCCACTCTACGACCATGTCCTCGCCCCCTTCGCCCGCAACCTCACCAACTCTGAGTCTGGTATCTCCCACCTCCAACGCATAGGTGTTGGCCTCCTCCTCTCCGTCCTCGCCATGGCCGTTGCAGCCCTTGTCGAGACCAAGCGTAAGGACCTCGCTCAAAACAGCACCGCTGAACCACTCCCCCTCACCTTCCTCTGGCTTGCATTCCAGTACCTTTTTCTGGGGTCGGCGGATTTGTTCACCTTGGCTGGCTTGTTGGAGTTTTTCTTCACGGAGGCGCCTACTGGGATGAGATCCTTGGCCACGTCACTATCTTGGGCTTCTTTGGCCGTCGGGTATTACCTGAGTTCGGTTATTGTGACGGTGGTGAACGGTGTGACCGCTAGATCCGGCCACACCCCATGGCTCTCCGGTGACAATATTAATCACTACCATCTTGACAGATTCTACTGGCTCATGTGCACGCTCACTGGATTGAACTTTTTGCACTACCTTTTCTGGGCTGTCAAGTACAAATATCGATCCCCCGCTGGAAACAAGTAATGCAAATTTTAATGCCTACAACATAGTTATATCATATATACTTTAACTTCAATGTAGTTTTAGCTTTTCAACTTATTTCTCATCCATGTAAGTTGGCCTTGTGCTAACATAAGAATCCACGAAAGAGAAAAATTAGTATATTTTAAAATGATTAAATCAGTCCAAGTGTCGAT

mRNA sequence

GAGGAGAGCGGGAGGAGATGGGAGGGGTACGTGGACTGGCGGAAGAGGGTGGCGGTGAGGGGGCGGCACGGGGGGATGGCGGCGGCGGGGTTCGTGCTGGGGGTGGAGATACTGGAGAACTTGGCGTTTCTGGCAAATGCAAGCAACTTGGTGATGTACTTGAGGAAGTACATGGGGTTTTCGCCGGAGAAATCGGCAAACCATGTCACGACTTTCATGGCCACAGCCTTCCTTCTCGCCCTTCTTGGTGGCTTCTTATCGGATGCCTTTTTCACCACTTATCACGTCTTCCTTTTCTCCTCCCTCATTGAGTTTCTGGGGTTGGTGATCTTGACAGTACAAGCGAAGGTCCCATCTCTAAAACCGGTGCCGTGCAAGGCGACGAATGGGAGCTCATGTAGGGAGGTGGGTGGGGGAGAGGCAGCGATGCTGTTCGTGGGGCTATACCTGGTGGCATTGGGGGTGGGCGGGATAAAGGGATCATTGCCGGCGCACGGGGCGGAGCAGTTCGACGAAGGCACGCCTCAAGGGCGTAGGAGGAGATCCACTTTCTTCAACTACTTCGTCTTCTGTCTCTCTTGTGGGGCTCTTGTGGCCGTCACTCTTGTGGTTTGGATCGAAGACAACTTGGGATGGGAGTGGGGCTTTGGAATCTCAACCATCACCATATTTCTCTCTATTCCTTTATTCCTTTCTGGATCCACCTTCTACCGAAACAAGATACCAACTGGAAGCCCCCTTACTACAATTCTAAAGGTTTTGGTGGCTGCAACTTTGAACCGTTGCTGCAGCAACAAATCATCGACCAACGCAGTGGCGAGCATGAGCACAAGCCCTTCCGTAACAACAACCCTCGAGTTGGAGGAAGGCCAATCCAATAATAATAACCCTGCCGAAACAATGAGTACAGCTCTAACTCCAACAAAAAGCCTAAAATTTCTCAACACAGCAGTAGAAAAACAGCCATTCCACCCATCCCTAAACTGCACAAACCAACAACTAGAAGAAGTGAAAACGGTTCTCAAAGTGCTCCCAATCTTTGCTTGCACCGTCATGCTCAACTGCTGCCTCGCCCAACTCTCGACCTTCTCCGTCCAACAAGCCTCCACAATGGACACCCGAATCGGCTCCCTCAAGCTCCCCCCAGCCTCACTCCCCGTCTTTCCCGTCCTCTTCATCATTGTCCTCGTCCCACTCTACGACCATGTCCTCGCCCCCTTCGCCCGCAACCTCACCAACTCTGAGTCTGGTATCTCCCACCTCCAACGCATAGGTGTTGGCCTCCTCCTCTCCGTCCTCGCCATGGCCGTTGCAGCCCTTGTCGAGACCAAGCGTAAGGACCTCGCTCAAAACAGCACCGCTGAACCACTCCCCCTCACCTTCCTCTGGCTTGCATTCCAGTACCTTTTTCTGGGGTCGGCGGATTTGTTCACCTTGGCTGGCTTGTTGGAGTTTTTCTTCACGGAGGCGCCTACTGGGATGAGATCCTTGGCCACGTCACTATCTTGGGCTTCTTTGGCCGTCGGGTATTACCTGAGTTCGGTTATTGTGACGGTGGTGAACGGTGTGACCGCTAGATCCGGCCACACCCCATGGCTCTCCGGTGACAATATTAATCACTACCATCTTGACAGATTCTACTGGCTCATGTGCACGCTCACTGGATTGAACTTTTTGCACTACCTTTTCTGGGCTGTCAAGTACAAATATCGATCCCCCGCTGGAAACAAGTAATGCAAATTTTAATGCCTACAACATAGTTATATCATATATACTTTAACTTCAATGTAGTTTTAGCTTTTCAACTTATTTCTCATCCATGTAAGTTGGCCTTGTGCTAACATAAGAATCCACGAAAGAGAAAAATTAGTATATTTTAAAATGATTAAATCAGTCCAAGTGTCGAT

Coding sequence (CDS)

GAGGAGAGCGGGAGGAGATGGGAGGGGTACGTGGACTGGCGGAAGAGGGTGGCGGTGAGGGGGCGGCACGGGGGGATGGCGGCGGCGGGGTTCGTGCTGGGGGTGGAGATACTGGAGAACTTGGCGTTTCTGGCAAATGCAAGCAACTTGGTGATGTACTTGAGGAAGTACATGGGGTTTTCGCCGGAGAAATCGGCAAACCATGTCACGACTTTCATGGCCACAGCCTTCCTTCTCGCCCTTCTTGGTGGCTTCTTATCGGATGCCTTTTTCACCACTTATCACGTCTTCCTTTTCTCCTCCCTCATTGAGTTTCTGGGGTTGGTGATCTTGACAGTACAAGCGAAGGTCCCATCTCTAAAACCGGTGCCGTGCAAGGCGACGAATGGGAGCTCATGTAGGGAGGTGGGTGGGGGAGAGGCAGCGATGCTGTTCGTGGGGCTATACCTGGTGGCATTGGGGGTGGGCGGGATAAAGGGATCATTGCCGGCGCACGGGGCGGAGCAGTTCGACGAAGGCACGCCTCAAGGGCGTAGGAGGAGATCCACTTTCTTCAACTACTTCGTCTTCTGTCTCTCTTGTGGGGCTCTTGTGGCCGTCACTCTTGTGGTTTGGATCGAAGACAACTTGGGATGGGAGTGGGGCTTTGGAATCTCAACCATCACCATATTTCTCTCTATTCCTTTATTCCTTTCTGGATCCACCTTCTACCGAAACAAGATACCAACTGGAAGCCCCCTTACTACAATTCTAAAGGTTTTGGTGGCTGCAACTTTGAACCGTTGCTGCAGCAACAAATCATCGACCAACGCAGTGGCGAGCATGAGCACAAGCCCTTCCGTAACAACAACCCTCGAGTTGGAGGAAGGCCAATCCAATAATAATAACCCTGCCGAAACAATGAGTACAGCTCTAACTCCAACAAAAAGCCTAAAATTTCTCAACACAGCAGTAGAAAAACAGCCATTCCACCCATCCCTAAACTGCACAAACCAACAACTAGAAGAAGTGAAAACGGTTCTCAAAGTGCTCCCAATCTTTGCTTGCACCGTCATGCTCAACTGCTGCCTCGCCCAACTCTCGACCTTCTCCGTCCAACAAGCCTCCACAATGGACACCCGAATCGGCTCCCTCAAGCTCCCCCCAGCCTCACTCCCCGTCTTTCCCGTCCTCTTCATCATTGTCCTCGTCCCACTCTACGACCATGTCCTCGCCCCCTTCGCCCGCAACCTCACCAACTCTGAGTCTGGTATCTCCCACCTCCAACGCATAGGTGTTGGCCTCCTCCTCTCCGTCCTCGCCATGGCCGTTGCAGCCCTTGTCGAGACCAAGCGTAAGGACCTCGCTCAAAACAGCACCGCTGAACCACTCCCCCTCACCTTCCTCTGGCTTGCATTCCAGTACCTTTTTCTGGGGTCGGCGGATTTGTTCACCTTGGCTGGCTTGTTGGAGTTTTTCTTCACGGAGGCGCCTACTGGGATGAGATCCTTGGCCACGTCACTATCTTGGGCTTCTTTGGCCGTCGGGTATTACCTGAGTTCGGTTATTGTGACGGTGGTGAACGGTGTGACCGCTAGATCCGGCCACACCCCATGGCTCTCCGGTGACAATATTAATCACTACCATCTTGACAGATTCTACTGGCTCATGTGCACGCTCACTGGATTGAACTTTTTGCACTACCTTTTCTGGGCTGTCAAGTACAAATATCGATCCCCCGCTGGAAACAAGTAA

Protein sequence

EESGRRWEGYVDWRKRVAVRGRHGGMAAAGFVLGVEILENLAFLANASNLVMYLRKYMGFSPEKSANHVTTFMATAFLLALLGGFLSDAFFTTYHVFLFSSLIEFLGLVILTVQAKVPSLKPVPCKATNGSSCREVGGGEAAMLFVGLYLVALGVGGIKGSLPAHGAEQFDEGTPQGRRRRSTFFNYFVFCLSCGALVAVTLVVWIEDNLGWEWGFGISTITIFLSIPLFLSGSTFYRNKIPTGSPLTTILKVLVAATLNRCCSNKSSTNAVASMSTSPSVTTTLELEEGQSNNNNPAETMSTALTPTKSLKFLNTAVEKQPFHPSLNCTNQQLEEVKTVLKVLPIFACTVMLNCCLAQLSTFSVQQASTMDTRIGSLKLPPASLPVFPVLFIIVLVPLYDHVLAPFARNLTNSESGISHLQRIGVGLLLSVLAMAVAALVETKRKDLAQNSTAEPLPLTFLWLAFQYLFLGSADLFTLAGLLEFFFTEAPTGMRSLATSLSWASLAVGYYLSSVIVTVVNGVTARSGHTPWLSGDNINHYHLDRFYWLMCTLTGLNFLHYLFWAVKYKYRSPAGNK
Homology
BLAST of MC08g1553 vs. ExPASy Swiss-Prot
Match: Q8H157 (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 2.2e-225
Identity = 408/583 (69.98%), Postives = 482/583 (82.68%), Query Frame = 0

Query: 1   EESGRRWEGYVDWRKRVAVRGRHGGMAAAGFVLGVEILENLAFLANASNLVMYLRKYMGF 60
           EE   RWEGY DWR R AV+GRHGGM AA FVL VEILENLA+LANASNLV+YLR+YM  
Sbjct: 4   EEEVSRWEGYADWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHM 63

Query: 61  SPEKSANHVTTFMATAFLLALLGGFLSDAFFTTYHVFLFSSLIEFLGLVILTVQAKVPSL 120
           SP KSAN VT FM TAFLLALLGGFLSDAFF+T+ +FL S+ IEFLGL+ILT+QA+ PSL
Sbjct: 64  SPSKSANDVTNFMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSL 123

Query: 121 KPVPCKATNGSSCREVGGGEAAMLFVGLYLVALGVGGIKGSLPAHGAEQFDEGTPQGRRR 180
            P  C   +  +C EV G +AAMLFVGLYLVALGVGGIKGSL +HGAEQFDE TP+GR++
Sbjct: 124 MPPSC---DSPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQ 183

Query: 181 RSTFFNYFVFCLSCGALVAVTLVVWIEDNLGWEWGFGISTITIFLSIPLFLSGSTFYRNK 240
           RSTFFNYFVFCL+CGALVAVT VVW+EDN GWEWGFG+STI IF+SI +FLSGS FYRNK
Sbjct: 184 RSTFFNYFVFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNK 243

Query: 241 IPTGSPLTTILKVLVAATLNRCCSNKSSTNAVASMSTSPS---VTTTLELEEGQSNNNNP 300
           IP GSPLTTILKVL+AA++ +CCS+ SS+NAVASMS SPS   V+   +  E Q     P
Sbjct: 244 IPCGSPLTTILKVLLAASV-KCCSSGSSSNAVASMSVSPSNHCVSKGKKEVESQGELEKP 303

Query: 301 AETMSTALTP----TKSLKFLNTAVEKQPFHPSLNCTNQQLEEVKTVLKVLPIFACTVML 360
            +    AL P    T SLK LN A +++P H  L CT QQ+E+VK VLK+LPIFACT+ML
Sbjct: 304 RQ--EEALPPRAQLTNSLKVLNGAADEKPVHRLLECTVQQVEDVKIVLKMLPIFACTIML 363

Query: 361 NCCLAQLSTFSVQQASTMDTRIGSLKLPPASLPVFPVLFIIVLVPLYDHVLAPFARNLTN 420
           NCCLAQLSTFSVQQA++M+T+IGSLK+PPASLP+FPV+FI++L P+YDH++ PFAR  T 
Sbjct: 364 NCCLAQLSTFSVQQAASMNTKIGSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKATK 423

Query: 421 SESGISHLQRIGVGLLLSVLAMAVAALVETKRKDLAQNS----TAEPLPLTFLWLAFQYL 480
           +E+G++HLQRIGVGL+LS+LAMAVAALVE KRK +A++S    + E LP+TFLW+A QYL
Sbjct: 424 TETGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQYL 483

Query: 481 FLGSADLFTLAGLLEFFFTEAPTGMRSLATSLSWASLAVGYYLSSVIVTVVNGVTARSGH 540
           FLGSADLFTLAGLLE+FFTEAP+ MRSLATSLSWASLA+GYYLSSVIV++VN +T  SG+
Sbjct: 484 FLGSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNSITGSSGN 543

Query: 541 TPWLSGDNINHYHLDRFYWLMCTLTGLNFLHYLFWAVKYKYRS 573
           TPWL G +IN Y LD FYWLMC L+  NFLHYLFWA++YKYRS
Sbjct: 544 TPWLRGKSINRYKLDYFYWLMCVLSAANFLHYLFWAMRYKYRS 580

BLAST of MC08g1553 vs. ExPASy Swiss-Prot
Match: Q8VYE4 (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 2.5e-205
Identity = 371/571 (64.97%), Postives = 455/571 (79.68%), Query Frame = 0

Query: 6   RWEGYVDWRKRVAVRGRHGGMAAAGFVLGVEILENLAFLANASNLVMYLRKYMGFSPEKS 65
           +WEGY DWR + A+RGRHGGM AA FVL VEILENLAFLANASNLV+YL+ +M  S  +S
Sbjct: 11  KWEGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARS 70

Query: 66  ANHVTTFMATAFLLALLGGFLSDAFFTTYHVFLFSSLIEFLGLVILTVQAKVPSLKPVPC 125
           ++ VTTFMATAFLLALLGGFL+DAFF+T+ +FL S+ IEFLGL++LT+QA+ PSL P PC
Sbjct: 71  SSEVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPC 130

Query: 126 KATNGSSCREVGGGEAAMLFVGLYLVALGVGGIKGSLPAHGAEQFDEGTPQGRRRRSTFF 185
           K++    C  VGG +AA LFVGLYLV+LG+GGIKGSLP+HGAEQFDEGTP+GR++RSTFF
Sbjct: 131 KSSAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFF 190

Query: 186 NYFVFCLSCGALVAVTLVVWIEDNLGWEWGFGISTITIFLSIPLFLSGSTFYRNKIPTGS 245
           NY+VFCLSCGALVAVT VVWIEDN GWEWGFG+STI+IFLSI +FL GS FY+NKIP GS
Sbjct: 191 NYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGS 250

Query: 246 PLTTILKVLVAATLNRCCSNKSSTNAVASMSTSPSVTTTLELEEGQSNNNNPAETMSTAL 305
           PLTTI KVL+AA++   CS+K+S+N   S              + +     P++++    
Sbjct: 251 PLTTIFKVLLAASI-VSCSSKTSSNHFTSREV-----------QSEHEEKTPSQSL---- 310

Query: 306 TPTKSLKFLNTAVEKQPFHPSLNCTNQQLEEVKTVLKVLPIFACTVMLNCCLAQLSTFSV 365
             T SL  LN A+E +  H  L CT QQ+E+VK VLK+LPIF CT+MLNCCLAQLST+SV
Sbjct: 311 --TNSLTCLNKAIEGKTHHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSV 370

Query: 366 QQASTMDTRIGSLKLPPASLPVFPVLFIIVLVPLYDHVLAPFARNLTNSESGISHLQRIG 425
            QA+TM+ +I +  +P ASLPVFPV+F+++L P YDH++ PFAR +T SE GI+HLQRIG
Sbjct: 371 HQAATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIG 430

Query: 426 VGLLLSVLAMAVAALVETKRKDLAQNS----TAEPLPLTFLWLAFQYLFLGSADLFTLAG 485
           VGL+LS++AMAVAALVE KRK +A+ +    + E LP+TFLW+A QYLFLGSADLFTLAG
Sbjct: 431 VGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAG 490

Query: 486 LLEFFFTEAPTGMRSLATSLSWASLAVGYYLSSVIVTVVNGVTARSGHTPWLSGDNINHY 545
           LLEFFFTEAP+ MRSLATSLSWASLA+GYYLSSV+V +VN VT  +G +PWL G+ +N  
Sbjct: 491 LLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWL-GEKLNRN 550

Query: 546 HLDRFYWLMCTLTGLNFLHYLFWAVKYKYRS 573
            LD FYWLMC L+ +NFLHYLFWA +YKY S
Sbjct: 551 RLDLFYWLMCVLSVVNFLHYLFWAKRYKYIS 562

BLAST of MC08g1553 vs. ExPASy Swiss-Prot
Match: Q9FM20 (Protein NRT1/ PTR FAMILY 4.7 OS=Arabidopsis thaliana OX=3702 GN=NPF4.7 PE=2 SV=2)

HSP 1 Score: 651.7 bits (1680), Expect = 7.5e-186
Identity = 343/579 (59.24%), Postives = 426/579 (73.58%), Query Frame = 0

Query: 7   WEGYVDWRKRVAVRGRHGGMAAAGFVLGVEILENLAFLANASNLVMYLRKYMGFSPEKSA 66
           W GYVDWR R A+RGRHGGM AA FVL VE+LENLAFLANASNLV+YL   MGFSP  +A
Sbjct: 41  WNGYVDWRSRPALRGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLSTKMGFSPSGAA 100

Query: 67  NHVTTFMATAFLLALLGGFLSDAFFTTYHVFLFSSLIEFLGLVILTVQAKVPSLKPVPCK 126
           N VT FM TAF LALLGGFL+DAFFTT+H++L S+ IEFLGL++LTVQA   S +P    
Sbjct: 101 NAVTAFMGTAFFLALLGGFLADAFFTTFHIYLVSAAIEFLGLMVLTVQAHEHSTEP---- 160

Query: 127 ATNGSSCREVGGGEAAMLFVGLYLVALGVGGIKGSLPAHGAEQFDEGTPQGRRRRSTFFN 186
                            LFVGLYLVALGVGGIKGSLP HGAEQFDE T  GRR+RS FFN
Sbjct: 161 ------------WSRVFLFVGLYLVALGVGGIKGSLPPHGAEQFDEETSSGRRQRSFFFN 220

Query: 187 YFVFCLSCGALVAVTLVVWIEDNLGWEWGFGISTITIFLSIPLFLSGSTFYRNKIPTGSP 246
           YF+F LSCGAL+AVT+VVW+EDN GW +GFG+ST  I +S+P+FL+GS  YR K+P+GSP
Sbjct: 221 YFIFSLSCGALIAVTVVVWLEDNKGWSYGFGVSTAAILISVPVFLAGSRVYRLKVPSGSP 280

Query: 247 LTTILKVLVAATLNRCCSNKSSTNAVASMSTSPSVTTTLELEEGQSNNNNPAETMSTALT 306
           +TT+ KVL AA   +    ++S   V       +  T  + ++  +  N   +       
Sbjct: 281 ITTLFKVLTAALYAKYKKRRTSRIVV-------TCHTRNDCDDSVTKQNCDGDDGFLG-- 340

Query: 307 PTKSLKFLNTAV-EKQPFHPSLNCTNQQLEEVKTVLKVLPIFACTVMLNCCLAQLSTFSV 366
                 FL   V E++     L CT +Q+++VK V+K+LPIF  T+MLNCCLAQLSTFSV
Sbjct: 341 -----SFLGEVVRERESLPRPLRCTEEQVKDVKIVIKILPIFMSTIMLNCCLAQLSTFSV 400

Query: 367 QQASTMDTRIGSLKLPPASLPVFPVLFIIVLVPLYDHVLAPFARNLTNSESGISHLQRIG 426
           QQASTM+T++GS  +PPA+LPVFPV+F+++L P Y+H+L P AR  T +E+GI+HLQRIG
Sbjct: 401 QQASTMNTKLGSFTVPPAALPVFPVVFMMILAPTYNHLLLPLARKSTKTETGITHLQRIG 460

Query: 427 VGLLLSVLAMAVAALVETKRKDL---------AQNSTAEPLPLTFLWLAFQYLFLGSADL 486
            GL+LS++AMAVAALVETKRK +         + + ++ PLP+TFLW+A QY+FLGSADL
Sbjct: 461 TGLVLSIVAMAVAALVETKRKHVVVSCCSNNNSSSYSSSPLPITFLWVAIQYVFLGSADL 520

Query: 487 FTLAGLLEFFFTEAPTGMRSLATSLSWASLAVGYYLSSVIVTVVNGVTARSGHTPWLSGD 546
           FTLAG++EFFFTEAP+ MRSLATSLSWASLA+GYY SSV+V+ VN VT  + H PWL G+
Sbjct: 521 FTLAGMMEFFFTEAPSTMRSLATSLSWASLAMGYYFSSVLVSAVNFVTGLNHHNPWLLGE 580

Query: 547 NINHYHLDRFYWLMCTLTGLNFLHYLFWAVKYKYRSPAG 576
           N+N YHL+RFYWLMC L+G+NFLHYLFWA +Y YRS  G
Sbjct: 581 NLNQYHLERFYWLMCVLSGINFLHYLFWASRYVYRSNQG 589

BLAST of MC08g1553 vs. ExPASy Swiss-Prot
Match: Q93VV5 (Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 7.0e-107
Identity = 234/565 (41.42%), Postives = 335/565 (59.29%), Query Frame = 0

Query: 8   EGYVDWRKRVAVRGRHGGMAAAGFVLGVEILENLAFLANASNLVMYLRKYMGFSPEKSAN 67
           E  VDWR R +   +HGGM AA FVLG++  E +   A  +NL+ Y+   M F   K+AN
Sbjct: 27  EESVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKAAN 86

Query: 68  HVTTFMATAFLLALLGGFLSDAFFTTYHVFLFSSLIEFLGLVILTVQAKVPSLKPVPCKA 127
            VT F+ T F+ ALLGG+LSDAF  ++   +    +E  G ++L+VQA +P LKP  C  
Sbjct: 87  IVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKCNP 146

Query: 128 TNGSSCREVGGGEAAMLFVGLYLVALGVGGIKGSLPAHGAEQFDEGTPQGRRRRSTFFNY 187
               +C E  G +A + F+ LYLVALG G +K ++ AHGA+QF +  P+  +R S++FN 
Sbjct: 147 LIDQTCEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSSYFNA 206

Query: 188 FVFCLSCGALVAVTLVVWIEDNLGWEWGFGISTITIFLSIPLFLSGSTFYRNKIPTGSPL 247
             F  S G L+A+TL+VW++ + G + GFG+S   + + I   +SG+ ++RNK P  S  
Sbjct: 207 AYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFRNKRPRRSIF 266

Query: 248 TTILKVLVAATLNRCCSNKSSTNAVASMSTSPSVTTTLELEEGQSNNNNPAETMSTALTP 307
           T I  V+VAA L R                SPS    L  +   +N+  P+ T    L  
Sbjct: 267 TPIAHVIVAAILKR-------------KLASPSDPRMLHGDHHVANDVVPSST----LPH 326

Query: 308 TKSLKFLNTAVEK-----QPFHPSLNCTNQQLEEVKTVLKVLPIFACTVMLNCCLAQLST 367
           T   +FL+ A  K         P   CT  Q+E+VKT++ ++PIFA T++ N  LAQL T
Sbjct: 327 TPRFRFLDKACIKIQDTNTKESPWRLCTVTQVEQVKTLISLVPIFASTIVFNTILAQLQT 386

Query: 368 FSVQQASTMDTRI-GSLKLPPASLPVFPVLFIIVLVPLYDHVLAPFARNLTNSESGISHL 427
           FSVQQ S+M+TR+  S  +PPASL   P + +I LVPLYD  L PFAR LT   SGI  L
Sbjct: 387 FSVQQGSSMNTRLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLVPFARKLTGHNSGIPPL 446

Query: 428 QRIGVGLLLSVLAMAVAALVETKRKDLAQNSTAEPLPLTFLWLAFQYLFLGSADLFTLAG 487
            RIG+GL LS  +M  AA++E KR+D   +S  +   L+  W+  Q+L  G +++FT  G
Sbjct: 447 TRIGIGLFLSTFSMVSAAMLEKKRRD---SSVLDGRILSIFWITPQFLIFGISEMFTAVG 506

Query: 488 LLEFFFTEAPTGMRSLATSLSWASLAVGYYLSSVIVTVVNGVTARSGHTP-WLSGDNINH 547
           L+EFF+ ++  GM S   +L++ S + G+Y SSV+V+VVN +T+ S  +  WL  +++N 
Sbjct: 507 LIEFFYKQSAKGMESFLMALTYCSYSFGFYFSSVLVSVVNKITSTSVDSKGWLGENDLNK 566

Query: 548 YHLDRFYWLMCTLTGLNFLHYLFWA 566
             LD FYWL+  L+ LNFL YLFW+
Sbjct: 567 DRLDLFYWLLAVLSLLNFLSYLFWS 571

BLAST of MC08g1553 vs. ExPASy Swiss-Prot
Match: Q9LSE8 (Protein NRT1/ PTR FAMILY 4.2 OS=Arabidopsis thaliana OX=3702 GN=NPF4.2 PE=2 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 2.3e-102
Identity = 222/566 (39.22%), Postives = 337/566 (59.54%), Query Frame = 0

Query: 8   EGYVDWRKRVAVRGRHGGMAAAGFVLGVEILENLAFLANASNLVMYLRKYMGFSPEKSAN 67
           E + DW+ + A+ G+HGG+ AA  V  V ++EN+ F+AN  N V Y    M ++P  +AN
Sbjct: 7   EKFEDWKGKEAIPGKHGGIRAASIVCVVVMMENIVFIANGFNFVKYFMGSMHYTPATAAN 66

Query: 68  HVTTFMATAFLLALLGGFLSDAFFTTYHVFLFSSLIEFLGLVILTVQAKVPSLKPVPCKA 127
            VT FM T+FLL L GGF++D+F T +  F+    IE +GL++LT QA  P L P   K 
Sbjct: 67  MVTNFMGTSFLLTLFGGFIADSFVTHFTTFIVFCCIELMGLILLTFQAHNPKLLPEKDKT 126

Query: 128 TNGSSCREVGGGEAAMLFVGLYLVALGVGGIKGSLPAHGAEQFDEGTPQGRRRRSTFFNY 187
            +          ++A+LF GLY +A+G GG+K SLP+HG +Q D   P   R  S FF++
Sbjct: 127 PSTL--------QSAILFTGLYAMAIGTGGLKASLPSHGGDQIDRRNP---RLISRFFDW 186

Query: 188 FVFCLSCGALVAVTLVVWIEDNLGWEWGFGISTITIFLSIPLFLSGSTFYRNKIPTGSPL 247
             F +  G L+AVT+V+WIE+  GW W F IS   +  ++ +F  G  FYR K P GSPL
Sbjct: 187 LYFSICSGCLLAVTVVLWIEEKKGWIWSFNISVGILATALCIFTVGLPFYRFKRPNGSPL 246

Query: 248 TTILKVLVAATLNRCCSNKSSTNAVASMSTSPSVTTTLELEEGQSNNNNPAETMSTALTP 307
             I  V+++A  NR   NKS  +          +   + + +  S+N             
Sbjct: 247 KKIAIVIISAARNR---NKSDLD-------EEMMRGLISIYKNNSHN------------- 306

Query: 308 TKSLKFLNTAVEKQPFHPSLNCTNQQLEEVKTVLKVLPIFACTVMLNCCLAQLSTFSVQQ 367
              LK+++ A   +      N +  ++EE +T L +LPIF  T++++CC+AQLSTFS QQ
Sbjct: 307 --KLKWIDKATLNK------NISETEVEETRTFLGLLPIFGSTIVMSCCVAQLSTFSAQQ 366

Query: 368 ASTMDTRI-GSLKLPPASLPVFPVLFIIVLVPLYDHVLAPFARNLT---NSESGISHLQR 427
              M+ ++  S ++P  SL   P++F+++ +PLY+     F + ++   N+ S   +L+R
Sbjct: 367 GMLMNKKLFHSFEIPVPSLTAIPLIFMLLSIPLYEF----FGKKISSGNNNRSSSFNLKR 426

Query: 428 IGVGLLLSVLAMAVAALVETKRK-DLAQNSTAEPLPLTFLWLAFQYLFLGSADLFTLAGL 487
           IG+GL LS ++MAV+A+VE KRK ++  N+      ++ LWL FQYL L  +D+ TL G+
Sbjct: 427 IGLGLALSSVSMAVSAIVEAKRKHEVVHNN----FRISVLWLVFQYLMLSVSDMLTLGGM 486

Query: 488 LEFFFTEAPTGMRSLATSLSWASLAVGYYLSSVIVTVVNGVTARSGHTPWLSGDNINHYH 547
           LEFF+ EAP+ M+S++T+L W S A+G++LS+ +V V N VT R GH  WL G+++N   
Sbjct: 487 LEFFYREAPSNMKSISTALGWCSTALGFFLSTTLVEVTNAVTGRLGH-QWLGGEDLNKTR 521

Query: 548 LDRFYWLMCTLTGLNFLHYLFWAVKY 569
           L+ FY L+C L  LN L+Y+FWA +Y
Sbjct: 547 LELFYVLLCVLNTLNLLNYIFWAKRY 521

BLAST of MC08g1553 vs. NCBI nr
Match: XP_022158071.1 (protein NRT1/ PTR FAMILY 4.6-like [Momordica charantia])

HSP 1 Score: 1118 bits (2892), Expect = 0.0
Identity = 577/577 (100.00%), Postives = 577/577 (100.00%), Query Frame = 0

Query: 1   EESGRRWEGYVDWRKRVAVRGRHGGMAAAGFVLGVEILENLAFLANASNLVMYLRKYMGF 60
           EESGRRWEGYVDWRKRVAVRGRHGGMAAAGFVLGVEILENLAFLANASNLVMYLRKYMGF
Sbjct: 5   EESGRRWEGYVDWRKRVAVRGRHGGMAAAGFVLGVEILENLAFLANASNLVMYLRKYMGF 64

Query: 61  SPEKSANHVTTFMATAFLLALLGGFLSDAFFTTYHVFLFSSLIEFLGLVILTVQAKVPSL 120
           SPEKSANHVTTFMATAFLLALLGGFLSDAFFTTYHVFLFSSLIEFLGLVILTVQAKVPSL
Sbjct: 65  SPEKSANHVTTFMATAFLLALLGGFLSDAFFTTYHVFLFSSLIEFLGLVILTVQAKVPSL 124

Query: 121 KPVPCKATNGSSCREVGGGEAAMLFVGLYLVALGVGGIKGSLPAHGAEQFDEGTPQGRRR 180
           KPVPCKATNGSSCREVGGGEAAMLFVGLYLVALGVGGIKGSLPAHGAEQFDEGTPQGRRR
Sbjct: 125 KPVPCKATNGSSCREVGGGEAAMLFVGLYLVALGVGGIKGSLPAHGAEQFDEGTPQGRRR 184

Query: 181 RSTFFNYFVFCLSCGALVAVTLVVWIEDNLGWEWGFGISTITIFLSIPLFLSGSTFYRNK 240
           RSTFFNYFVFCLSCGALVAVTLVVWIEDNLGWEWGFGISTITIFLSIPLFLSGSTFYRNK
Sbjct: 185 RSTFFNYFVFCLSCGALVAVTLVVWIEDNLGWEWGFGISTITIFLSIPLFLSGSTFYRNK 244

Query: 241 IPTGSPLTTILKVLVAATLNRCCSNKSSTNAVASMSTSPSVTTTLELEEGQSNNNNPAET 300
           IPTGSPLTTILKVLVAATLNRCCSNKSSTNAVASMSTSPSVTTTLELEEGQSNNNNPAET
Sbjct: 245 IPTGSPLTTILKVLVAATLNRCCSNKSSTNAVASMSTSPSVTTTLELEEGQSNNNNPAET 304

Query: 301 MSTALTPTKSLKFLNTAVEKQPFHPSLNCTNQQLEEVKTVLKVLPIFACTVMLNCCLAQL 360
           MSTALTPTKSLKFLNTAVEKQPFHPSLNCTNQQLEEVKTVLKVLPIFACTVMLNCCLAQL
Sbjct: 305 MSTALTPTKSLKFLNTAVEKQPFHPSLNCTNQQLEEVKTVLKVLPIFACTVMLNCCLAQL 364

Query: 361 STFSVQQASTMDTRIGSLKLPPASLPVFPVLFIIVLVPLYDHVLAPFARNLTNSESGISH 420
           STFSVQQASTMDTRIGSLKLPPASLPVFPVLFIIVLVPLYDHVLAPFARNLTNSESGISH
Sbjct: 365 STFSVQQASTMDTRIGSLKLPPASLPVFPVLFIIVLVPLYDHVLAPFARNLTNSESGISH 424

Query: 421 LQRIGVGLLLSVLAMAVAALVETKRKDLAQNSTAEPLPLTFLWLAFQYLFLGSADLFTLA 480
           LQRIGVGLLLSVLAMAVAALVETKRKDLAQNSTAEPLPLTFLWLAFQYLFLGSADLFTLA
Sbjct: 425 LQRIGVGLLLSVLAMAVAALVETKRKDLAQNSTAEPLPLTFLWLAFQYLFLGSADLFTLA 484

Query: 481 GLLEFFFTEAPTGMRSLATSLSWASLAVGYYLSSVIVTVVNGVTARSGHTPWLSGDNINH 540
           GLLEFFFTEAPTGMRSLATSLSWASLAVGYYLSSVIVTVVNGVTARSGHTPWLSGDNINH
Sbjct: 485 GLLEFFFTEAPTGMRSLATSLSWASLAVGYYLSSVIVTVVNGVTARSGHTPWLSGDNINH 544

Query: 541 YHLDRFYWLMCTLTGLNFLHYLFWAVKYKYRSPAGNK 577
           YHLDRFYWLMCTLTGLNFLHYLFWAVKYKYRSPAGNK
Sbjct: 545 YHLDRFYWLMCTLTGLNFLHYLFWAVKYKYRSPAGNK 581

BLAST of MC08g1553 vs. NCBI nr
Match: XP_038886228.1 (protein NRT1/ PTR FAMILY 4.6-like [Benincasa hispida])

HSP 1 Score: 869 bits (2245), Expect = 1.66e-313
Identity = 451/578 (78.03%), Postives = 500/578 (86.51%), Query Frame = 0

Query: 1   EESGRRWEGYVDWRKRVAVRGRHGGMAAAGFVLGVEILENLAFLANASNLVMYLRKYMGF 60
           E  G  WEGYVDWRKR AVRGRHGGM AAGFVLGVE+LENLAFLANASNLVMYLRKYM F
Sbjct: 7   ESDGSIWEGYVDWRKRAAVRGRHGGMMAAGFVLGVEVLENLAFLANASNLVMYLRKYMEF 66

Query: 61  SPEKSANHVTTFMATAFLLALLGGFLSDAFFTTYHVFLFSSLIEFLGLVILTVQAKVPSL 120
           SP KSANHVTTFM TAFLLALLGGFLSDAFFTTY+VFLFSSL+EFLGLV+LTVQAKV S 
Sbjct: 67  SPAKSANHVTTFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVMLTVQAKVSS- 126

Query: 121 KPVPCKATNGSSCREVGGGEAAMLFVGLYLVALGVGGIKGSLPAHGAEQFDEGTPQGRRR 180
               C+  N   C+EVGG EAA+LF+GLYLVALGVGGIKGSLPAHGAEQFDEG+PQGR+R
Sbjct: 127 ---SCEEGNSKGCKEVGGREAAVLFIGLYLVALGVGGIKGSLPAHGAEQFDEGSPQGRKR 186

Query: 181 RSTFFNYFVFCLSCGALVAVTLVVWIEDNLGWEWGFGISTITIFLSIPLFLSGSTFYRNK 240
           RS+FFNYFVF LSCGAL+AVT VVW+EDNLGW+WGFGISTI+IFLSIPLFL+GS FYRNK
Sbjct: 187 RSSFFNYFVFSLSCGALIAVTFVVWVEDNLGWQWGFGISTISIFLSIPLFLAGSPFYRNK 246

Query: 241 IPTGSPLTTILKVLVAATLNRCCSNKSSTNAVASMSTSPSVTTTLELEEGQSNNNNPAET 300
           IPTGSPLTTILKVLVAA LNR  SNK +TNAVAS++ SPS +T  +L   +   NN    
Sbjct: 247 IPTGSPLTTILKVLVAAALNRGSSNKPATNAVASLARSPSTSTAKQLINKELTQNNTTN- 306

Query: 301 MSTALTPTKSLKFLNTAVEKQPFHPSLNCTNQQLEEVKTVLKVLPIFACTVMLNCCLAQL 360
                 PT+SLKFLN AV+  PFHPSL CT QQLE+VK VLKVLPIFACT++LN CLAQL
Sbjct: 307 ------PTQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVLKVLPIFACTIILNSCLAQL 366

Query: 361 STFSVQQASTMDTRIGSLKLPPASLPVFPVLFIIVLVPLYDHVLAPFARNLTNSESGISH 420
           STFS++QASTM+T+I SLK+PPASLP+FPVLFII+L PLYDH++ PFAR LT +E+GI+H
Sbjct: 367 STFSIEQASTMNTKISSLKVPPASLPIFPVLFIILLAPLYDHLIIPFARTLTRTEAGITH 426

Query: 421 LQRIGVGLLLSVLAMAVAALVETKRKDLA-QNSTAEPLPLTFLWLAFQYLFLGSADLFTL 480
           LQRIGVGLLLSVLAMAVAALVETKRK +A  +  A PLP+TFLW+AFQYLFLGSADLFTL
Sbjct: 427 LQRIGVGLLLSVLAMAVAALVETKRKAVAGAHPDAHPLPITFLWIAFQYLFLGSADLFTL 486

Query: 481 AGLLEFFFTEAPTGMRSLATSLSWASLAVGYYLSSVIVTVVNGVTARSGHTPWLSGDNIN 540
           AGLLEFFFTEAP  MRSLAT+LSWASLAVGYYLSSVIV+VVN VTA++ HTPWLSG NIN
Sbjct: 487 AGLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNRVTAKASHTPWLSGHNIN 546

Query: 541 HYHLDRFYWLMCTLTGLNFLHYLFWAVKYKYRSPAGNK 577
           HY L  FYWLMC L+G NFLHYLFWA+KYKYRS  GNK
Sbjct: 547 HYRLSNFYWLMCALSGFNFLHYLFWALKYKYRSTQGNK 573

BLAST of MC08g1553 vs. NCBI nr
Match: XP_011657481.1 (protein NRT1/ PTR FAMILY 4.6 [Cucumis sativus] >APY23675.1 NRT1.2-like nitrate transporter [Cucumis sativus] >KAE8647291.1 hypothetical protein Csa_004297 [Cucumis sativus])

HSP 1 Score: 850 bits (2195), Expect = 7.25e-306
Identity = 443/581 (76.25%), Postives = 499/581 (85.89%), Query Frame = 0

Query: 1   EESGRRWEGYVDWRKRVAVRGRHGGMAAAGFVLGVEILENLAFLANASNLVMYLRKYMGF 60
           E  G  WEGYVDWRKR A +GRHGGM AAGFVLGVE+LENLAFLANASNLVMYLRKYMGF
Sbjct: 7   ESDGSIWEGYVDWRKRPAAKGRHGGMIAAGFVLGVEVLENLAFLANASNLVMYLRKYMGF 66

Query: 61  SPEKSANHVTTFMATAFLLALLGGFLSDAFFTTYHVFLFSSLIEFLGLVILTVQAKVPSL 120
           SP KSANHVTTFM TAFLLALLGGFLSDA FTTY+VF+FSS IEFLGLV+LTVQAKV S 
Sbjct: 67  SPAKSANHVTTFMGTAFLLALLGGFLSDAIFTTYYVFIFSSFIEFLGLVMLTVQAKVSS- 126

Query: 121 KPVPCKATNGSSCREVGGGEAAMLFVGLYLVALGVGGIKGSLPAHGAEQFDEGTPQGRRR 180
               C+  +   C+EVGG E A+LF+GLYLVALGVGGIKGSLPAHGAEQFDE TPQGR+R
Sbjct: 127 ---SCEEGSSKGCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTPQGRKR 186

Query: 181 RSTFFNYFVFCLSCGALVAVTLVVWIEDNLGWEWGFGISTITIFLSIPLFLSGSTFYRNK 240
           RS+FFNYFVF LSCGAL+AVT VVWIEDNLGW+WGFGISTI+IFLSIPLF +GS FYRNK
Sbjct: 187 RSSFFNYFVFSLSCGALIAVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAGSPFYRNK 246

Query: 241 IPTGSPLTTILKVLVAATLNRCCSNKSSTNAVASMSTSPSVTTTLE--LEEGQSNNNNPA 300
           IPTGSPLTTILKVLVAATLNR  + K +TNAVAS++ SPS T+T +  + +  + NN   
Sbjct: 247 IPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKQLINKDLTENN--- 306

Query: 301 ETMSTALTPTKSLKFLNTAVEKQPFHPSLNCTNQQLEEVKTVLKVLPIFACTVMLNCCLA 360
                  TPT+SLKFLN AV+  PFHPSL CT QQLE+VK V+KVLPIFACT++LN CLA
Sbjct: 307 -----VTTPTQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVIKVLPIFACTIILNSCLA 366

Query: 361 QLSTFSVQQASTMDTRIGSLKLPPASLPVFPVLFIIVLVPLYDHVLAPFARNLTNSESGI 420
           QLSTFS++QA+TM+T+I SLK+PPASLPVFPVLFII+L PLYDH++ PFAR+LT +E+GI
Sbjct: 367 QLSTFSIEQAATMNTKIYSLKIPPASLPVFPVLFIIILAPLYDHLIIPFARSLTRTEAGI 426

Query: 421 SHLQRIGVGLLLSVLAMAVAALVETKRKDLAQNS--TAEPLPLTFLWLAFQYLFLGSADL 480
           +HLQRIGVGLLLSVLAMAVAALVETKRK +A  +     PLP+TFLW+AFQYLFLGSADL
Sbjct: 427 THLQRIGVGLLLSVLAMAVAALVETKRKAVADANPDVHGPLPITFLWIAFQYLFLGSADL 486

Query: 481 FTLAGLLEFFFTEAPTGMRSLATSLSWASLAVGYYLSSVIVTVVNGVTARSGHTPWLSGD 540
           FTLAGLLEFFFTEAPT MRSLAT+LSWASLAVGYYLSSVIV+VVN V+A++ H PWLSG 
Sbjct: 487 FTLAGLLEFFFTEAPTTMRSLATALSWASLAVGYYLSSVIVSVVNHVSAKAWHRPWLSGH 546

Query: 541 NINHYHLDRFYWLMCTLTGLNFLHYLFWAVKYKYRSPAGNK 577
           NINHY L  FYW+MC L+GLNFLHYLFWA+KYKYRS  GNK
Sbjct: 547 NINHYRLSNFYWVMCVLSGLNFLHYLFWALKYKYRSTQGNK 575

BLAST of MC08g1553 vs. NCBI nr
Match: XP_008456458.1 (PREDICTED: protein NRT1/ PTR FAMILY 4.6-like [Cucumis melo] >KAA0036440.1 protein NRT1/ PTR FAMILY 4.6-like [Cucumis melo var. makuwa] >TYK00047.1 protein NRT1/ PTR FAMILY 4.6-like [Cucumis melo var. makuwa])

HSP 1 Score: 843 bits (2179), Expect = 1.90e-303
Identity = 445/580 (76.72%), Postives = 497/580 (85.69%), Query Frame = 0

Query: 1   EESGRRWEGYVDWRKRVAVRGRHGGMAAAGFVLGVEILENLAFLANASNLVMYLRKYMGF 60
           E  G  WEGYVDWRKR AV+GRHGGM AAGFVLGVE+LENLAFLANASNLVMYLRKYMGF
Sbjct: 7   ESDGSIWEGYVDWRKRPAVKGRHGGMLAAGFVLGVEVLENLAFLANASNLVMYLRKYMGF 66

Query: 61  SPEKSANHVTTFMATAFLLALLGGFLSDAFFTTYHVFLFSSLIEFLGLVILTVQAKVPSL 120
           SP KSANHVTTFM TAFLLALLGGFLSDAFFTTY+VFLFSS +EFLGLV+LTVQAKV S 
Sbjct: 67  SPAKSANHVTTFMGTAFLLALLGGFLSDAFFTTYYVFLFSSFLEFLGLVMLTVQAKVSS- 126

Query: 121 KPVPCKA-TNGSSCREVGGGEAAMLFVGLYLVALGVGGIKGSLPAHGAEQFDEGTPQGRR 180
               C+A T G  C+EVGG E A+LF+GLYLVALGVGGIKGSLPAHGAEQFDE T QGR+
Sbjct: 127 ---SCEAGTKG--CKEVGGKEVAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTTQGRK 186

Query: 181 RRSTFFNYFVFCLSCGALVAVTLVVWIEDNLGWEWGFGISTITIFLSIPLFLSGSTFYRN 240
           RRS+FFNYFVF LSCGALVAVT VVW+EDNLGW+WGFGISTI+ FLSIPLF +GS FYRN
Sbjct: 187 RRSSFFNYFVFSLSCGALVAVTFVVWVEDNLGWQWGFGISTISTFLSIPLFFAGSPFYRN 246

Query: 241 KIPTGSPLTTILKVLVAATLNRCCSNKSSTNAVASMSTSPSVTTTLELEEGQSNNNNPAE 300
           KIPTGSPLTTILKVLVAATLNR  + K +TNAVAS++ SPS T+T +         N   
Sbjct: 247 KIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKPLI------NKDL 306

Query: 301 TMSTALTPTKSLKFLNTAVEKQPFHPSLNCTNQQLEEVKTVLKVLPIFACTVMLNCCLAQ 360
           T S   TPT+SLKFLN AV+  PFHPS+ CT QQLE+VK V+KVLPIFACT++LN CLAQ
Sbjct: 307 TESNTTTPTQSLKFLNKAVQNPPFHPSVKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQ 366

Query: 361 LSTFSVQQASTMDTRIGSLKLPPASLPVFPVLFIIVLVPLYDHVLAPFARNLTNSESGIS 420
           LSTFS++QA+TM+T+I SLK+PPASLPVFPVLFIIVL PLYDH++ PFAR+LT +E+GI+
Sbjct: 367 LSTFSIEQAATMNTKISSLKIPPASLPVFPVLFIIVLAPLYDHLIIPFARSLTRTEAGIT 426

Query: 421 HLQRIGVGLLLSVLAMAVAALVETKRKDLAQNS--TAEPLPLTFLWLAFQYLFLGSADLF 480
           HLQRIGVGLLLSVLAMAVAALVETKRK +A  +     PLP+TFLW+AFQYLFLGSADLF
Sbjct: 427 HLQRIGVGLLLSVLAMAVAALVETKRKAVADANPDVQGPLPITFLWIAFQYLFLGSADLF 486

Query: 481 TLAGLLEFFFTEAPTGMRSLATSLSWASLAVGYYLSSVIVTVVNGVTARSGHTPWLSGDN 540
           TLAGLLEFFFTEAP  MRSLAT+LSWASLAVGYYLSSVIV+VVN V+A++ H PWLSG N
Sbjct: 487 TLAGLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNHVSAKAWHRPWLSGHN 546

Query: 541 INHYHLDRFYWLMCTLTGLNFLHYLFWAVKYKYRSPAGNK 577
           INHY L  FYW+MC L+G NFLHYLFWA+KYKYRS  GNK
Sbjct: 547 INHYRLSNFYWVMCVLSGFNFLHYLFWALKYKYRSTQGNK 574

BLAST of MC08g1553 vs. NCBI nr
Match: KAB1220739.1 (Protein NRT1/ PTR FAMILY 4.6 [Morella rubra])

HSP 1 Score: 820 bits (2119), Expect = 7.37e-291
Identity = 422/577 (73.14%), Postives = 489/577 (84.75%), Query Frame = 0

Query: 6   RWEGYVDWRKRVAVRGRHGGMAAAGFVLGVEILENLAFLANASNLVMYLRKYMGFSPEKS 65
           RWEGYVDWRKR A+ GRHGGM AA FVL  EILENLA+LANASNLV+YL +YM FSP KS
Sbjct: 224 RWEGYVDWRKRPALSGRHGGMIAASFVLVAEILENLAYLANASNLVLYLSEYMNFSPSKS 283

Query: 66  ANHVTTFMATAFLLALLGGFLSDAFFTTYHVFLFSSLIEFLGLVILTVQAKVPSLKPVPC 125
           AN+VT FM TAFLLALLGGFL+DAFFTTYH++L S++IEFLGLV+LTVQAK PSLKP  C
Sbjct: 284 ANNVTDFMGTAFLLALLGGFLADAFFTTYHIYLISAVIEFLGLVVLTVQAKSPSLKPPAC 343

Query: 126 KATNGS-SCREVGGGEAAMLFVGLYLVALGVGGIKGSLPAHGAEQFDEGTPQGRRRRSTF 185
             TN + +C+EV GG+AAMLF+GLYLVALGVGGIKGSLPAHGAEQFDE TPQGR++RSTF
Sbjct: 344 DPTNRNIACQEVHGGKAAMLFLGLYLVALGVGGIKGSLPAHGAEQFDESTPQGRKQRSTF 403

Query: 186 FNYFVFCLSCGALVAVTLVVWIEDNLGWEWGFGISTITIFLSIPLFLSGSTFYRNKIPTG 245
           FNYFVFCLSCGAL+AVTLVVWIEDN GWEWGFG+STI+IFLSIP+FL+GS  YRNKIP+G
Sbjct: 404 FNYFVFCLSCGALIAVTLVVWIEDNKGWEWGFGVSTISIFLSIPVFLAGSATYRNKIPSG 463

Query: 246 SPLTTILKVLVAATLNRCCSNKSSTNAVASMSTSPSVTTTLELEEGQSNNNNPAETMSTA 305
           SPLTTILKVLVAATLNRC S +S  NAV SM+T PS +  L  +E +  +    E  + A
Sbjct: 464 SPLTTILKVLVAATLNRCVS-RSPRNAVVSMATCPS-SPNLTSKEAEEQSTKTREESTNA 523

Query: 306 LTPTKSLKFLNTAVEKQPFHPSLNCTNQQLEEVKTVLKVLPIFACTVMLNCCLAQLSTFS 365
             PT+SLKFLN AV  +P H +L CT QQ+E+VK VLKVLPIF CT+MLNCCLAQLSTFS
Sbjct: 524 --PTESLKFLNKAVVNKPAHKALECTVQQVEDVKVVLKVLPIFVCTIMLNCCLAQLSTFS 583

Query: 366 VQQASTMDTRIGSLKLPPASLPVFPVLFIIVLVPLYDHVLAPFARNLTNSESGISHLQRI 425
           VQQA++MDT++GS+K+PPASLPVFPV FII+L P+YDH++ PFAR  T SE GI+HLQRI
Sbjct: 584 VQQAASMDTKLGSMKVPPASLPVFPVTFIIILAPVYDHLIIPFARKATKSEMGITHLQRI 643

Query: 426 GVGLLLSVLAMAVAALVETKRKDLAQNS----TAEPLPLTFLWLAFQYLFLGSADLFTLA 485
           G+GL+LS++AMAVAALVE KRK +A +S    +  PLP+TFLW+AFQYLFLGSADLFTLA
Sbjct: 644 GIGLVLSIIAMAVAALVEIKRKRVATHSGLLDSEHPLPITFLWIAFQYLFLGSADLFTLA 703

Query: 486 GLLEFFFTEAPTGMRSLATSLSWASLAVGYYLSSVIVTVVNGVTARSGHTPWLSGDNINH 545
           GLLEFFFTEAP  MRSLATSLSWASLA+GYYLSSVIV++VN +T  S H  WLSG N+NH
Sbjct: 704 GLLEFFFTEAPISMRSLATSLSWASLAMGYYLSSVIVSIVNSITGHSKHRAWLSGSNLNH 763

Query: 546 YHLDRFYWLMCTLTGLNFLHYLFWAVKYKYRSPAGNK 577
           YHL+RFYWL+C L+ LNFLH+LFWA  YKY+SP   K
Sbjct: 764 YHLERFYWLLCVLSALNFLHFLFWATPYKYKSPGAQK 796

BLAST of MC08g1553 vs. ExPASy TrEMBL
Match: A0A6J1DZY8 (protein NRT1/ PTR FAMILY 4.6-like OS=Momordica charantia OX=3673 GN=LOC111024644 PE=3 SV=1)

HSP 1 Score: 1118 bits (2892), Expect = 0.0
Identity = 577/577 (100.00%), Postives = 577/577 (100.00%), Query Frame = 0

Query: 1   EESGRRWEGYVDWRKRVAVRGRHGGMAAAGFVLGVEILENLAFLANASNLVMYLRKYMGF 60
           EESGRRWEGYVDWRKRVAVRGRHGGMAAAGFVLGVEILENLAFLANASNLVMYLRKYMGF
Sbjct: 5   EESGRRWEGYVDWRKRVAVRGRHGGMAAAGFVLGVEILENLAFLANASNLVMYLRKYMGF 64

Query: 61  SPEKSANHVTTFMATAFLLALLGGFLSDAFFTTYHVFLFSSLIEFLGLVILTVQAKVPSL 120
           SPEKSANHVTTFMATAFLLALLGGFLSDAFFTTYHVFLFSSLIEFLGLVILTVQAKVPSL
Sbjct: 65  SPEKSANHVTTFMATAFLLALLGGFLSDAFFTTYHVFLFSSLIEFLGLVILTVQAKVPSL 124

Query: 121 KPVPCKATNGSSCREVGGGEAAMLFVGLYLVALGVGGIKGSLPAHGAEQFDEGTPQGRRR 180
           KPVPCKATNGSSCREVGGGEAAMLFVGLYLVALGVGGIKGSLPAHGAEQFDEGTPQGRRR
Sbjct: 125 KPVPCKATNGSSCREVGGGEAAMLFVGLYLVALGVGGIKGSLPAHGAEQFDEGTPQGRRR 184

Query: 181 RSTFFNYFVFCLSCGALVAVTLVVWIEDNLGWEWGFGISTITIFLSIPLFLSGSTFYRNK 240
           RSTFFNYFVFCLSCGALVAVTLVVWIEDNLGWEWGFGISTITIFLSIPLFLSGSTFYRNK
Sbjct: 185 RSTFFNYFVFCLSCGALVAVTLVVWIEDNLGWEWGFGISTITIFLSIPLFLSGSTFYRNK 244

Query: 241 IPTGSPLTTILKVLVAATLNRCCSNKSSTNAVASMSTSPSVTTTLELEEGQSNNNNPAET 300
           IPTGSPLTTILKVLVAATLNRCCSNKSSTNAVASMSTSPSVTTTLELEEGQSNNNNPAET
Sbjct: 245 IPTGSPLTTILKVLVAATLNRCCSNKSSTNAVASMSTSPSVTTTLELEEGQSNNNNPAET 304

Query: 301 MSTALTPTKSLKFLNTAVEKQPFHPSLNCTNQQLEEVKTVLKVLPIFACTVMLNCCLAQL 360
           MSTALTPTKSLKFLNTAVEKQPFHPSLNCTNQQLEEVKTVLKVLPIFACTVMLNCCLAQL
Sbjct: 305 MSTALTPTKSLKFLNTAVEKQPFHPSLNCTNQQLEEVKTVLKVLPIFACTVMLNCCLAQL 364

Query: 361 STFSVQQASTMDTRIGSLKLPPASLPVFPVLFIIVLVPLYDHVLAPFARNLTNSESGISH 420
           STFSVQQASTMDTRIGSLKLPPASLPVFPVLFIIVLVPLYDHVLAPFARNLTNSESGISH
Sbjct: 365 STFSVQQASTMDTRIGSLKLPPASLPVFPVLFIIVLVPLYDHVLAPFARNLTNSESGISH 424

Query: 421 LQRIGVGLLLSVLAMAVAALVETKRKDLAQNSTAEPLPLTFLWLAFQYLFLGSADLFTLA 480
           LQRIGVGLLLSVLAMAVAALVETKRKDLAQNSTAEPLPLTFLWLAFQYLFLGSADLFTLA
Sbjct: 425 LQRIGVGLLLSVLAMAVAALVETKRKDLAQNSTAEPLPLTFLWLAFQYLFLGSADLFTLA 484

Query: 481 GLLEFFFTEAPTGMRSLATSLSWASLAVGYYLSSVIVTVVNGVTARSGHTPWLSGDNINH 540
           GLLEFFFTEAPTGMRSLATSLSWASLAVGYYLSSVIVTVVNGVTARSGHTPWLSGDNINH
Sbjct: 485 GLLEFFFTEAPTGMRSLATSLSWASLAVGYYLSSVIVTVVNGVTARSGHTPWLSGDNINH 544

Query: 541 YHLDRFYWLMCTLTGLNFLHYLFWAVKYKYRSPAGNK 577
           YHLDRFYWLMCTLTGLNFLHYLFWAVKYKYRSPAGNK
Sbjct: 545 YHLDRFYWLMCTLTGLNFLHYLFWAVKYKYRSPAGNK 581

BLAST of MC08g1553 vs. ExPASy TrEMBL
Match: A0A1P8SN46 (NRT1.2-like nitrate transporter OS=Cucumis sativus OX=3659 GN=NRT1.2C PE=2 SV=1)

HSP 1 Score: 850 bits (2195), Expect = 3.51e-306
Identity = 443/581 (76.25%), Postives = 499/581 (85.89%), Query Frame = 0

Query: 1   EESGRRWEGYVDWRKRVAVRGRHGGMAAAGFVLGVEILENLAFLANASNLVMYLRKYMGF 60
           E  G  WEGYVDWRKR A +GRHGGM AAGFVLGVE+LENLAFLANASNLVMYLRKYMGF
Sbjct: 7   ESDGSIWEGYVDWRKRPAAKGRHGGMIAAGFVLGVEVLENLAFLANASNLVMYLRKYMGF 66

Query: 61  SPEKSANHVTTFMATAFLLALLGGFLSDAFFTTYHVFLFSSLIEFLGLVILTVQAKVPSL 120
           SP KSANHVTTFM TAFLLALLGGFLSDA FTTY+VF+FSS IEFLGLV+LTVQAKV S 
Sbjct: 67  SPAKSANHVTTFMGTAFLLALLGGFLSDAIFTTYYVFIFSSFIEFLGLVMLTVQAKVSS- 126

Query: 121 KPVPCKATNGSSCREVGGGEAAMLFVGLYLVALGVGGIKGSLPAHGAEQFDEGTPQGRRR 180
               C+  +   C+EVGG E A+LF+GLYLVALGVGGIKGSLPAHGAEQFDE TPQGR+R
Sbjct: 127 ---SCEEGSSKGCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTPQGRKR 186

Query: 181 RSTFFNYFVFCLSCGALVAVTLVVWIEDNLGWEWGFGISTITIFLSIPLFLSGSTFYRNK 240
           RS+FFNYFVF LSCGAL+AVT VVWIEDNLGW+WGFGISTI+IFLSIPLF +GS FYRNK
Sbjct: 187 RSSFFNYFVFSLSCGALIAVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAGSPFYRNK 246

Query: 241 IPTGSPLTTILKVLVAATLNRCCSNKSSTNAVASMSTSPSVTTTLE--LEEGQSNNNNPA 300
           IPTGSPLTTILKVLVAATLNR  + K +TNAVAS++ SPS T+T +  + +  + NN   
Sbjct: 247 IPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKQLINKDLTENN--- 306

Query: 301 ETMSTALTPTKSLKFLNTAVEKQPFHPSLNCTNQQLEEVKTVLKVLPIFACTVMLNCCLA 360
                  TPT+SLKFLN AV+  PFHPSL CT QQLE+VK V+KVLPIFACT++LN CLA
Sbjct: 307 -----VTTPTQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVIKVLPIFACTIILNSCLA 366

Query: 361 QLSTFSVQQASTMDTRIGSLKLPPASLPVFPVLFIIVLVPLYDHVLAPFARNLTNSESGI 420
           QLSTFS++QA+TM+T+I SLK+PPASLPVFPVLFII+L PLYDH++ PFAR+LT +E+GI
Sbjct: 367 QLSTFSIEQAATMNTKIYSLKIPPASLPVFPVLFIIILAPLYDHLIIPFARSLTRTEAGI 426

Query: 421 SHLQRIGVGLLLSVLAMAVAALVETKRKDLAQNS--TAEPLPLTFLWLAFQYLFLGSADL 480
           +HLQRIGVGLLLSVLAMAVAALVETKRK +A  +     PLP+TFLW+AFQYLFLGSADL
Sbjct: 427 THLQRIGVGLLLSVLAMAVAALVETKRKAVADANPDVHGPLPITFLWIAFQYLFLGSADL 486

Query: 481 FTLAGLLEFFFTEAPTGMRSLATSLSWASLAVGYYLSSVIVTVVNGVTARSGHTPWLSGD 540
           FTLAGLLEFFFTEAPT MRSLAT+LSWASLAVGYYLSSVIV+VVN V+A++ H PWLSG 
Sbjct: 487 FTLAGLLEFFFTEAPTTMRSLATALSWASLAVGYYLSSVIVSVVNHVSAKAWHRPWLSGH 546

Query: 541 NINHYHLDRFYWLMCTLTGLNFLHYLFWAVKYKYRSPAGNK 577
           NINHY L  FYW+MC L+GLNFLHYLFWA+KYKYRS  GNK
Sbjct: 547 NINHYRLSNFYWVMCVLSGLNFLHYLFWALKYKYRSTQGNK 575

BLAST of MC08g1553 vs. ExPASy TrEMBL
Match: A0A5D3BLL0 (Protein NRT1/ PTR FAMILY 4.6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold360G00950 PE=3 SV=1)

HSP 1 Score: 843 bits (2179), Expect = 9.19e-304
Identity = 445/580 (76.72%), Postives = 497/580 (85.69%), Query Frame = 0

Query: 1   EESGRRWEGYVDWRKRVAVRGRHGGMAAAGFVLGVEILENLAFLANASNLVMYLRKYMGF 60
           E  G  WEGYVDWRKR AV+GRHGGM AAGFVLGVE+LENLAFLANASNLVMYLRKYMGF
Sbjct: 7   ESDGSIWEGYVDWRKRPAVKGRHGGMLAAGFVLGVEVLENLAFLANASNLVMYLRKYMGF 66

Query: 61  SPEKSANHVTTFMATAFLLALLGGFLSDAFFTTYHVFLFSSLIEFLGLVILTVQAKVPSL 120
           SP KSANHVTTFM TAFLLALLGGFLSDAFFTTY+VFLFSS +EFLGLV+LTVQAKV S 
Sbjct: 67  SPAKSANHVTTFMGTAFLLALLGGFLSDAFFTTYYVFLFSSFLEFLGLVMLTVQAKVSS- 126

Query: 121 KPVPCKA-TNGSSCREVGGGEAAMLFVGLYLVALGVGGIKGSLPAHGAEQFDEGTPQGRR 180
               C+A T G  C+EVGG E A+LF+GLYLVALGVGGIKGSLPAHGAEQFDE T QGR+
Sbjct: 127 ---SCEAGTKG--CKEVGGKEVAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTTQGRK 186

Query: 181 RRSTFFNYFVFCLSCGALVAVTLVVWIEDNLGWEWGFGISTITIFLSIPLFLSGSTFYRN 240
           RRS+FFNYFVF LSCGALVAVT VVW+EDNLGW+WGFGISTI+ FLSIPLF +GS FYRN
Sbjct: 187 RRSSFFNYFVFSLSCGALVAVTFVVWVEDNLGWQWGFGISTISTFLSIPLFFAGSPFYRN 246

Query: 241 KIPTGSPLTTILKVLVAATLNRCCSNKSSTNAVASMSTSPSVTTTLELEEGQSNNNNPAE 300
           KIPTGSPLTTILKVLVAATLNR  + K +TNAVAS++ SPS T+T +         N   
Sbjct: 247 KIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKPLI------NKDL 306

Query: 301 TMSTALTPTKSLKFLNTAVEKQPFHPSLNCTNQQLEEVKTVLKVLPIFACTVMLNCCLAQ 360
           T S   TPT+SLKFLN AV+  PFHPS+ CT QQLE+VK V+KVLPIFACT++LN CLAQ
Sbjct: 307 TESNTTTPTQSLKFLNKAVQNPPFHPSVKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQ 366

Query: 361 LSTFSVQQASTMDTRIGSLKLPPASLPVFPVLFIIVLVPLYDHVLAPFARNLTNSESGIS 420
           LSTFS++QA+TM+T+I SLK+PPASLPVFPVLFIIVL PLYDH++ PFAR+LT +E+GI+
Sbjct: 367 LSTFSIEQAATMNTKISSLKIPPASLPVFPVLFIIVLAPLYDHLIIPFARSLTRTEAGIT 426

Query: 421 HLQRIGVGLLLSVLAMAVAALVETKRKDLAQNS--TAEPLPLTFLWLAFQYLFLGSADLF 480
           HLQRIGVGLLLSVLAMAVAALVETKRK +A  +     PLP+TFLW+AFQYLFLGSADLF
Sbjct: 427 HLQRIGVGLLLSVLAMAVAALVETKRKAVADANPDVQGPLPITFLWIAFQYLFLGSADLF 486

Query: 481 TLAGLLEFFFTEAPTGMRSLATSLSWASLAVGYYLSSVIVTVVNGVTARSGHTPWLSGDN 540
           TLAGLLEFFFTEAP  MRSLAT+LSWASLAVGYYLSSVIV+VVN V+A++ H PWLSG N
Sbjct: 487 TLAGLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNHVSAKAWHRPWLSGHN 546

Query: 541 INHYHLDRFYWLMCTLTGLNFLHYLFWAVKYKYRSPAGNK 577
           INHY L  FYW+MC L+G NFLHYLFWA+KYKYRS  GNK
Sbjct: 547 INHYRLSNFYWVMCVLSGFNFLHYLFWALKYKYRSTQGNK 574

BLAST of MC08g1553 vs. ExPASy TrEMBL
Match: A0A1S3C3A1 (protein NRT1/ PTR FAMILY 4.6-like OS=Cucumis melo OX=3656 GN=LOC103496401 PE=3 SV=1)

HSP 1 Score: 843 bits (2179), Expect = 9.19e-304
Identity = 445/580 (76.72%), Postives = 497/580 (85.69%), Query Frame = 0

Query: 1   EESGRRWEGYVDWRKRVAVRGRHGGMAAAGFVLGVEILENLAFLANASNLVMYLRKYMGF 60
           E  G  WEGYVDWRKR AV+GRHGGM AAGFVLGVE+LENLAFLANASNLVMYLRKYMGF
Sbjct: 7   ESDGSIWEGYVDWRKRPAVKGRHGGMLAAGFVLGVEVLENLAFLANASNLVMYLRKYMGF 66

Query: 61  SPEKSANHVTTFMATAFLLALLGGFLSDAFFTTYHVFLFSSLIEFLGLVILTVQAKVPSL 120
           SP KSANHVTTFM TAFLLALLGGFLSDAFFTTY+VFLFSS +EFLGLV+LTVQAKV S 
Sbjct: 67  SPAKSANHVTTFMGTAFLLALLGGFLSDAFFTTYYVFLFSSFLEFLGLVMLTVQAKVSS- 126

Query: 121 KPVPCKA-TNGSSCREVGGGEAAMLFVGLYLVALGVGGIKGSLPAHGAEQFDEGTPQGRR 180
               C+A T G  C+EVGG E A+LF+GLYLVALGVGGIKGSLPAHGAEQFDE T QGR+
Sbjct: 127 ---SCEAGTKG--CKEVGGKEVAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTTQGRK 186

Query: 181 RRSTFFNYFVFCLSCGALVAVTLVVWIEDNLGWEWGFGISTITIFLSIPLFLSGSTFYRN 240
           RRS+FFNYFVF LSCGALVAVT VVW+EDNLGW+WGFGISTI+ FLSIPLF +GS FYRN
Sbjct: 187 RRSSFFNYFVFSLSCGALVAVTFVVWVEDNLGWQWGFGISTISTFLSIPLFFAGSPFYRN 246

Query: 241 KIPTGSPLTTILKVLVAATLNRCCSNKSSTNAVASMSTSPSVTTTLELEEGQSNNNNPAE 300
           KIPTGSPLTTILKVLVAATLNR  + K +TNAVAS++ SPS T+T +         N   
Sbjct: 247 KIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKPLI------NKDL 306

Query: 301 TMSTALTPTKSLKFLNTAVEKQPFHPSLNCTNQQLEEVKTVLKVLPIFACTVMLNCCLAQ 360
           T S   TPT+SLKFLN AV+  PFHPS+ CT QQLE+VK V+KVLPIFACT++LN CLAQ
Sbjct: 307 TESNTTTPTQSLKFLNKAVQNPPFHPSVKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQ 366

Query: 361 LSTFSVQQASTMDTRIGSLKLPPASLPVFPVLFIIVLVPLYDHVLAPFARNLTNSESGIS 420
           LSTFS++QA+TM+T+I SLK+PPASLPVFPVLFIIVL PLYDH++ PFAR+LT +E+GI+
Sbjct: 367 LSTFSIEQAATMNTKISSLKIPPASLPVFPVLFIIVLAPLYDHLIIPFARSLTRTEAGIT 426

Query: 421 HLQRIGVGLLLSVLAMAVAALVETKRKDLAQNS--TAEPLPLTFLWLAFQYLFLGSADLF 480
           HLQRIGVGLLLSVLAMAVAALVETKRK +A  +     PLP+TFLW+AFQYLFLGSADLF
Sbjct: 427 HLQRIGVGLLLSVLAMAVAALVETKRKAVADANPDVQGPLPITFLWIAFQYLFLGSADLF 486

Query: 481 TLAGLLEFFFTEAPTGMRSLATSLSWASLAVGYYLSSVIVTVVNGVTARSGHTPWLSGDN 540
           TLAGLLEFFFTEAP  MRSLAT+LSWASLAVGYYLSSVIV+VVN V+A++ H PWLSG N
Sbjct: 487 TLAGLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNHVSAKAWHRPWLSGHN 546

Query: 541 INHYHLDRFYWLMCTLTGLNFLHYLFWAVKYKYRSPAGNK 577
           INHY L  FYW+MC L+G NFLHYLFWA+KYKYRS  GNK
Sbjct: 547 INHYRLSNFYWVMCVLSGFNFLHYLFWALKYKYRSTQGNK 574

BLAST of MC08g1553 vs. ExPASy TrEMBL
Match: A0A6A1W647 (Protein NRT1/ PTR FAMILY 4.6 OS=Morella rubra OX=262757 GN=CJ030_MR3G002950 PE=3 SV=1)

HSP 1 Score: 820 bits (2119), Expect = 3.57e-291
Identity = 422/577 (73.14%), Postives = 489/577 (84.75%), Query Frame = 0

Query: 6   RWEGYVDWRKRVAVRGRHGGMAAAGFVLGVEILENLAFLANASNLVMYLRKYMGFSPEKS 65
           RWEGYVDWRKR A+ GRHGGM AA FVL  EILENLA+LANASNLV+YL +YM FSP KS
Sbjct: 224 RWEGYVDWRKRPALSGRHGGMIAASFVLVAEILENLAYLANASNLVLYLSEYMNFSPSKS 283

Query: 66  ANHVTTFMATAFLLALLGGFLSDAFFTTYHVFLFSSLIEFLGLVILTVQAKVPSLKPVPC 125
           AN+VT FM TAFLLALLGGFL+DAFFTTYH++L S++IEFLGLV+LTVQAK PSLKP  C
Sbjct: 284 ANNVTDFMGTAFLLALLGGFLADAFFTTYHIYLISAVIEFLGLVVLTVQAKSPSLKPPAC 343

Query: 126 KATNGS-SCREVGGGEAAMLFVGLYLVALGVGGIKGSLPAHGAEQFDEGTPQGRRRRSTF 185
             TN + +C+EV GG+AAMLF+GLYLVALGVGGIKGSLPAHGAEQFDE TPQGR++RSTF
Sbjct: 344 DPTNRNIACQEVHGGKAAMLFLGLYLVALGVGGIKGSLPAHGAEQFDESTPQGRKQRSTF 403

Query: 186 FNYFVFCLSCGALVAVTLVVWIEDNLGWEWGFGISTITIFLSIPLFLSGSTFYRNKIPTG 245
           FNYFVFCLSCGAL+AVTLVVWIEDN GWEWGFG+STI+IFLSIP+FL+GS  YRNKIP+G
Sbjct: 404 FNYFVFCLSCGALIAVTLVVWIEDNKGWEWGFGVSTISIFLSIPVFLAGSATYRNKIPSG 463

Query: 246 SPLTTILKVLVAATLNRCCSNKSSTNAVASMSTSPSVTTTLELEEGQSNNNNPAETMSTA 305
           SPLTTILKVLVAATLNRC S +S  NAV SM+T PS +  L  +E +  +    E  + A
Sbjct: 464 SPLTTILKVLVAATLNRCVS-RSPRNAVVSMATCPS-SPNLTSKEAEEQSTKTREESTNA 523

Query: 306 LTPTKSLKFLNTAVEKQPFHPSLNCTNQQLEEVKTVLKVLPIFACTVMLNCCLAQLSTFS 365
             PT+SLKFLN AV  +P H +L CT QQ+E+VK VLKVLPIF CT+MLNCCLAQLSTFS
Sbjct: 524 --PTESLKFLNKAVVNKPAHKALECTVQQVEDVKVVLKVLPIFVCTIMLNCCLAQLSTFS 583

Query: 366 VQQASTMDTRIGSLKLPPASLPVFPVLFIIVLVPLYDHVLAPFARNLTNSESGISHLQRI 425
           VQQA++MDT++GS+K+PPASLPVFPV FII+L P+YDH++ PFAR  T SE GI+HLQRI
Sbjct: 584 VQQAASMDTKLGSMKVPPASLPVFPVTFIIILAPVYDHLIIPFARKATKSEMGITHLQRI 643

Query: 426 GVGLLLSVLAMAVAALVETKRKDLAQNS----TAEPLPLTFLWLAFQYLFLGSADLFTLA 485
           G+GL+LS++AMAVAALVE KRK +A +S    +  PLP+TFLW+AFQYLFLGSADLFTLA
Sbjct: 644 GIGLVLSIIAMAVAALVEIKRKRVATHSGLLDSEHPLPITFLWIAFQYLFLGSADLFTLA 703

Query: 486 GLLEFFFTEAPTGMRSLATSLSWASLAVGYYLSSVIVTVVNGVTARSGHTPWLSGDNINH 545
           GLLEFFFTEAP  MRSLATSLSWASLA+GYYLSSVIV++VN +T  S H  WLSG N+NH
Sbjct: 704 GLLEFFFTEAPISMRSLATSLSWASLAMGYYLSSVIVSIVNSITGHSKHRAWLSGSNLNH 763

Query: 546 YHLDRFYWLMCTLTGLNFLHYLFWAVKYKYRSPAGNK 577
           YHL+RFYWL+C L+ LNFLH+LFWA  YKY+SP   K
Sbjct: 764 YHLERFYWLLCVLSALNFLHFLFWATPYKYKSPGAQK 796

BLAST of MC08g1553 vs. TAIR 10
Match: AT1G69850.1 (nitrate transporter 1:2 )

HSP 1 Score: 783.1 bits (2021), Expect = 1.5e-226
Identity = 408/583 (69.98%), Postives = 482/583 (82.68%), Query Frame = 0

Query: 1   EESGRRWEGYVDWRKRVAVRGRHGGMAAAGFVLGVEILENLAFLANASNLVMYLRKYMGF 60
           EE   RWEGY DWR R AV+GRHGGM AA FVL VEILENLA+LANASNLV+YLR+YM  
Sbjct: 4   EEEVSRWEGYADWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHM 63

Query: 61  SPEKSANHVTTFMATAFLLALLGGFLSDAFFTTYHVFLFSSLIEFLGLVILTVQAKVPSL 120
           SP KSAN VT FM TAFLLALLGGFLSDAFF+T+ +FL S+ IEFLGL+ILT+QA+ PSL
Sbjct: 64  SPSKSANDVTNFMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSL 123

Query: 121 KPVPCKATNGSSCREVGGGEAAMLFVGLYLVALGVGGIKGSLPAHGAEQFDEGTPQGRRR 180
            P  C   +  +C EV G +AAMLFVGLYLVALGVGGIKGSL +HGAEQFDE TP+GR++
Sbjct: 124 MPPSC---DSPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQ 183

Query: 181 RSTFFNYFVFCLSCGALVAVTLVVWIEDNLGWEWGFGISTITIFLSIPLFLSGSTFYRNK 240
           RSTFFNYFVFCL+CGALVAVT VVW+EDN GWEWGFG+STI IF+SI +FLSGS FYRNK
Sbjct: 184 RSTFFNYFVFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNK 243

Query: 241 IPTGSPLTTILKVLVAATLNRCCSNKSSTNAVASMSTSPS---VTTTLELEEGQSNNNNP 300
           IP GSPLTTILKVL+AA++ +CCS+ SS+NAVASMS SPS   V+   +  E Q     P
Sbjct: 244 IPCGSPLTTILKVLLAASV-KCCSSGSSSNAVASMSVSPSNHCVSKGKKEVESQGELEKP 303

Query: 301 AETMSTALTP----TKSLKFLNTAVEKQPFHPSLNCTNQQLEEVKTVLKVLPIFACTVML 360
            +    AL P    T SLK LN A +++P H  L CT QQ+E+VK VLK+LPIFACT+ML
Sbjct: 304 RQ--EEALPPRAQLTNSLKVLNGAADEKPVHRLLECTVQQVEDVKIVLKMLPIFACTIML 363

Query: 361 NCCLAQLSTFSVQQASTMDTRIGSLKLPPASLPVFPVLFIIVLVPLYDHVLAPFARNLTN 420
           NCCLAQLSTFSVQQA++M+T+IGSLK+PPASLP+FPV+FI++L P+YDH++ PFAR  T 
Sbjct: 364 NCCLAQLSTFSVQQAASMNTKIGSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKATK 423

Query: 421 SESGISHLQRIGVGLLLSVLAMAVAALVETKRKDLAQNS----TAEPLPLTFLWLAFQYL 480
           +E+G++HLQRIGVGL+LS+LAMAVAALVE KRK +A++S    + E LP+TFLW+A QYL
Sbjct: 424 TETGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQYL 483

Query: 481 FLGSADLFTLAGLLEFFFTEAPTGMRSLATSLSWASLAVGYYLSSVIVTVVNGVTARSGH 540
           FLGSADLFTLAGLLE+FFTEAP+ MRSLATSLSWASLA+GYYLSSVIV++VN +T  SG+
Sbjct: 484 FLGSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNSITGSSGN 543

Query: 541 TPWLSGDNINHYHLDRFYWLMCTLTGLNFLHYLFWAVKYKYRS 573
           TPWL G +IN Y LD FYWLMC L+  NFLHYLFWA++YKYRS
Sbjct: 544 TPWLRGKSINRYKLDYFYWLMCVLSAANFLHYLFWAMRYKYRS 580

BLAST of MC08g1553 vs. TAIR 10
Match: AT1G27040.2 (Major facilitator superfamily protein )

HSP 1 Score: 716.5 bits (1848), Expect = 1.8e-206
Identity = 371/571 (64.97%), Postives = 455/571 (79.68%), Query Frame = 0

Query: 6   RWEGYVDWRKRVAVRGRHGGMAAAGFVLGVEILENLAFLANASNLVMYLRKYMGFSPEKS 65
           +WEGY DWR + A+RGRHGGM AA FVL VEILENLAFLANASNLV+YL+ +M  S  +S
Sbjct: 7   KWEGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARS 66

Query: 66  ANHVTTFMATAFLLALLGGFLSDAFFTTYHVFLFSSLIEFLGLVILTVQAKVPSLKPVPC 125
           ++ VTTFMATAFLLALLGGFL+DAFF+T+ +FL S+ IEFLGL++LT+QA+ PSL P PC
Sbjct: 67  SSEVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPC 126

Query: 126 KATNGSSCREVGGGEAAMLFVGLYLVALGVGGIKGSLPAHGAEQFDEGTPQGRRRRSTFF 185
           K++    C  VGG +AA LFVGLYLV+LG+GGIKGSLP+HGAEQFDEGTP+GR++RSTFF
Sbjct: 127 KSSAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFF 186

Query: 186 NYFVFCLSCGALVAVTLVVWIEDNLGWEWGFGISTITIFLSIPLFLSGSTFYRNKIPTGS 245
           NY+VFCLSCGALVAVT VVWIEDN GWEWGFG+STI+IFLSI +FL GS FY+NKIP GS
Sbjct: 187 NYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGS 246

Query: 246 PLTTILKVLVAATLNRCCSNKSSTNAVASMSTSPSVTTTLELEEGQSNNNNPAETMSTAL 305
           PLTTI KVL+AA++   CS+K+S+N   S              + +     P++++    
Sbjct: 247 PLTTIFKVLLAASI-VSCSSKTSSNHFTSREV-----------QSEHEEKTPSQSL---- 306

Query: 306 TPTKSLKFLNTAVEKQPFHPSLNCTNQQLEEVKTVLKVLPIFACTVMLNCCLAQLSTFSV 365
             T SL  LN A+E +  H  L CT QQ+E+VK VLK+LPIF CT+MLNCCLAQLST+SV
Sbjct: 307 --TNSLTCLNKAIEGKTHHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSV 366

Query: 366 QQASTMDTRIGSLKLPPASLPVFPVLFIIVLVPLYDHVLAPFARNLTNSESGISHLQRIG 425
            QA+TM+ +I +  +P ASLPVFPV+F+++L P YDH++ PFAR +T SE GI+HLQRIG
Sbjct: 367 HQAATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIG 426

Query: 426 VGLLLSVLAMAVAALVETKRKDLAQNS----TAEPLPLTFLWLAFQYLFLGSADLFTLAG 485
           VGL+LS++AMAVAALVE KRK +A+ +    + E LP+TFLW+A QYLFLGSADLFTLAG
Sbjct: 427 VGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAG 486

Query: 486 LLEFFFTEAPTGMRSLATSLSWASLAVGYYLSSVIVTVVNGVTARSGHTPWLSGDNINHY 545
           LLEFFFTEAP+ MRSLATSLSWASLA+GYYLSSV+V +VN VT  +G +PWL G+ +N  
Sbjct: 487 LLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWL-GEKLNRN 546

Query: 546 HLDRFYWLMCTLTGLNFLHYLFWAVKYKYRS 573
            LD FYWLMC L+ +NFLHYLFWA +YKY S
Sbjct: 547 RLDLFYWLMCVLSVVNFLHYLFWAKRYKYIS 558

BLAST of MC08g1553 vs. TAIR 10
Match: AT1G27040.1 (Major facilitator superfamily protein )

HSP 1 Score: 716.5 bits (1848), Expect = 1.8e-206
Identity = 371/571 (64.97%), Postives = 455/571 (79.68%), Query Frame = 0

Query: 6   RWEGYVDWRKRVAVRGRHGGMAAAGFVLGVEILENLAFLANASNLVMYLRKYMGFSPEKS 65
           +WEGY DWR + A+RGRHGGM AA FVL VEILENLAFLANASNLV+YL+ +M  S  +S
Sbjct: 11  KWEGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARS 70

Query: 66  ANHVTTFMATAFLLALLGGFLSDAFFTTYHVFLFSSLIEFLGLVILTVQAKVPSLKPVPC 125
           ++ VTTFMATAFLLALLGGFL+DAFF+T+ +FL S+ IEFLGL++LT+QA+ PSL P PC
Sbjct: 71  SSEVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPC 130

Query: 126 KATNGSSCREVGGGEAAMLFVGLYLVALGVGGIKGSLPAHGAEQFDEGTPQGRRRRSTFF 185
           K++    C  VGG +AA LFVGLYLV+LG+GGIKGSLP+HGAEQFDEGTP+GR++RSTFF
Sbjct: 131 KSSAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFF 190

Query: 186 NYFVFCLSCGALVAVTLVVWIEDNLGWEWGFGISTITIFLSIPLFLSGSTFYRNKIPTGS 245
           NY+VFCLSCGALVAVT VVWIEDN GWEWGFG+STI+IFLSI +FL GS FY+NKIP GS
Sbjct: 191 NYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGS 250

Query: 246 PLTTILKVLVAATLNRCCSNKSSTNAVASMSTSPSVTTTLELEEGQSNNNNPAETMSTAL 305
           PLTTI KVL+AA++   CS+K+S+N   S              + +     P++++    
Sbjct: 251 PLTTIFKVLLAASI-VSCSSKTSSNHFTSREV-----------QSEHEEKTPSQSL---- 310

Query: 306 TPTKSLKFLNTAVEKQPFHPSLNCTNQQLEEVKTVLKVLPIFACTVMLNCCLAQLSTFSV 365
             T SL  LN A+E +  H  L CT QQ+E+VK VLK+LPIF CT+MLNCCLAQLST+SV
Sbjct: 311 --TNSLTCLNKAIEGKTHHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSV 370

Query: 366 QQASTMDTRIGSLKLPPASLPVFPVLFIIVLVPLYDHVLAPFARNLTNSESGISHLQRIG 425
            QA+TM+ +I +  +P ASLPVFPV+F+++L P YDH++ PFAR +T SE GI+HLQRIG
Sbjct: 371 HQAATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIG 430

Query: 426 VGLLLSVLAMAVAALVETKRKDLAQNS----TAEPLPLTFLWLAFQYLFLGSADLFTLAG 485
           VGL+LS++AMAVAALVE KRK +A+ +    + E LP+TFLW+A QYLFLGSADLFTLAG
Sbjct: 431 VGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAG 490

Query: 486 LLEFFFTEAPTGMRSLATSLSWASLAVGYYLSSVIVTVVNGVTARSGHTPWLSGDNINHY 545
           LLEFFFTEAP+ MRSLATSLSWASLA+GYYLSSV+V +VN VT  +G +PWL G+ +N  
Sbjct: 491 LLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWL-GEKLNRN 550

Query: 546 HLDRFYWLMCTLTGLNFLHYLFWAVKYKYRS 573
            LD FYWLMC L+ +NFLHYLFWA +YKY S
Sbjct: 551 RLDLFYWLMCVLSVVNFLHYLFWAKRYKYIS 562

BLAST of MC08g1553 vs. TAIR 10
Match: AT5G62730.1 (Major facilitator superfamily protein )

HSP 1 Score: 651.7 bits (1680), Expect = 5.3e-187
Identity = 343/579 (59.24%), Postives = 426/579 (73.58%), Query Frame = 0

Query: 7   WEGYVDWRKRVAVRGRHGGMAAAGFVLGVEILENLAFLANASNLVMYLRKYMGFSPEKSA 66
           W GYVDWR R A+RGRHGGM AA FVL VE+LENLAFLANASNLV+YL   MGFSP  +A
Sbjct: 41  WNGYVDWRSRPALRGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLSTKMGFSPSGAA 100

Query: 67  NHVTTFMATAFLLALLGGFLSDAFFTTYHVFLFSSLIEFLGLVILTVQAKVPSLKPVPCK 126
           N VT FM TAF LALLGGFL+DAFFTT+H++L S+ IEFLGL++LTVQA   S +P    
Sbjct: 101 NAVTAFMGTAFFLALLGGFLADAFFTTFHIYLVSAAIEFLGLMVLTVQAHEHSTEP---- 160

Query: 127 ATNGSSCREVGGGEAAMLFVGLYLVALGVGGIKGSLPAHGAEQFDEGTPQGRRRRSTFFN 186
                            LFVGLYLVALGVGGIKGSLP HGAEQFDE T  GRR+RS FFN
Sbjct: 161 ------------WSRVFLFVGLYLVALGVGGIKGSLPPHGAEQFDEETSSGRRQRSFFFN 220

Query: 187 YFVFCLSCGALVAVTLVVWIEDNLGWEWGFGISTITIFLSIPLFLSGSTFYRNKIPTGSP 246
           YF+F LSCGAL+AVT+VVW+EDN GW +GFG+ST  I +S+P+FL+GS  YR K+P+GSP
Sbjct: 221 YFIFSLSCGALIAVTVVVWLEDNKGWSYGFGVSTAAILISVPVFLAGSRVYRLKVPSGSP 280

Query: 247 LTTILKVLVAATLNRCCSNKSSTNAVASMSTSPSVTTTLELEEGQSNNNNPAETMSTALT 306
           +TT+ KVL AA   +    ++S   V       +  T  + ++  +  N   +       
Sbjct: 281 ITTLFKVLTAALYAKYKKRRTSRIVV-------TCHTRNDCDDSVTKQNCDGDDGFLG-- 340

Query: 307 PTKSLKFLNTAV-EKQPFHPSLNCTNQQLEEVKTVLKVLPIFACTVMLNCCLAQLSTFSV 366
                 FL   V E++     L CT +Q+++VK V+K+LPIF  T+MLNCCLAQLSTFSV
Sbjct: 341 -----SFLGEVVRERESLPRPLRCTEEQVKDVKIVIKILPIFMSTIMLNCCLAQLSTFSV 400

Query: 367 QQASTMDTRIGSLKLPPASLPVFPVLFIIVLVPLYDHVLAPFARNLTNSESGISHLQRIG 426
           QQASTM+T++GS  +PPA+LPVFPV+F+++L P Y+H+L P AR  T +E+GI+HLQRIG
Sbjct: 401 QQASTMNTKLGSFTVPPAALPVFPVVFMMILAPTYNHLLLPLARKSTKTETGITHLQRIG 460

Query: 427 VGLLLSVLAMAVAALVETKRKDL---------AQNSTAEPLPLTFLWLAFQYLFLGSADL 486
            GL+LS++AMAVAALVETKRK +         + + ++ PLP+TFLW+A QY+FLGSADL
Sbjct: 461 TGLVLSIVAMAVAALVETKRKHVVVSCCSNNNSSSYSSSPLPITFLWVAIQYVFLGSADL 520

Query: 487 FTLAGLLEFFFTEAPTGMRSLATSLSWASLAVGYYLSSVIVTVVNGVTARSGHTPWLSGD 546
           FTLAG++EFFFTEAP+ MRSLATSLSWASLA+GYY SSV+V+ VN VT  + H PWL G+
Sbjct: 521 FTLAGMMEFFFTEAPSTMRSLATSLSWASLAMGYYFSSVLVSAVNFVTGLNHHNPWLLGE 580

Query: 547 NINHYHLDRFYWLMCTLTGLNFLHYLFWAVKYKYRSPAG 576
           N+N YHL+RFYWLMC L+G+NFLHYLFWA +Y YRS  G
Sbjct: 581 NLNQYHLERFYWLMCVLSGINFLHYLFWASRYVYRSNQG 589

BLAST of MC08g1553 vs. TAIR 10
Match: AT1G59740.1 (Major facilitator superfamily protein )

HSP 1 Score: 389.4 bits (999), Expect = 4.9e-108
Identity = 234/565 (41.42%), Postives = 335/565 (59.29%), Query Frame = 0

Query: 8   EGYVDWRKRVAVRGRHGGMAAAGFVLGVEILENLAFLANASNLVMYLRKYMGFSPEKSAN 67
           E  VDWR R +   +HGGM AA FVLG++  E +   A  +NL+ Y+   M F   K+AN
Sbjct: 27  EESVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKAAN 86

Query: 68  HVTTFMATAFLLALLGGFLSDAFFTTYHVFLFSSLIEFLGLVILTVQAKVPSLKPVPCKA 127
            VT F+ T F+ ALLGG+LSDAF  ++   +    +E  G ++L+VQA +P LKP  C  
Sbjct: 87  IVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKCNP 146

Query: 128 TNGSSCREVGGGEAAMLFVGLYLVALGVGGIKGSLPAHGAEQFDEGTPQGRRRRSTFFNY 187
               +C E  G +A + F+ LYLVALG G +K ++ AHGA+QF +  P+  +R S++FN 
Sbjct: 147 LIDQTCEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSSYFNA 206

Query: 188 FVFCLSCGALVAVTLVVWIEDNLGWEWGFGISTITIFLSIPLFLSGSTFYRNKIPTGSPL 247
             F  S G L+A+TL+VW++ + G + GFG+S   + + I   +SG+ ++RNK P  S  
Sbjct: 207 AYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFRNKRPRRSIF 266

Query: 248 TTILKVLVAATLNRCCSNKSSTNAVASMSTSPSVTTTLELEEGQSNNNNPAETMSTALTP 307
           T I  V+VAA L R                SPS    L  +   +N+  P+ T    L  
Sbjct: 267 TPIAHVIVAAILKR-------------KLASPSDPRMLHGDHHVANDVVPSST----LPH 326

Query: 308 TKSLKFLNTAVEK-----QPFHPSLNCTNQQLEEVKTVLKVLPIFACTVMLNCCLAQLST 367
           T   +FL+ A  K         P   CT  Q+E+VKT++ ++PIFA T++ N  LAQL T
Sbjct: 327 TPRFRFLDKACIKIQDTNTKESPWRLCTVTQVEQVKTLISLVPIFASTIVFNTILAQLQT 386

Query: 368 FSVQQASTMDTRI-GSLKLPPASLPVFPVLFIIVLVPLYDHVLAPFARNLTNSESGISHL 427
           FSVQQ S+M+TR+  S  +PPASL   P + +I LVPLYD  L PFAR LT   SGI  L
Sbjct: 387 FSVQQGSSMNTRLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLVPFARKLTGHNSGIPPL 446

Query: 428 QRIGVGLLLSVLAMAVAALVETKRKDLAQNSTAEPLPLTFLWLAFQYLFLGSADLFTLAG 487
            RIG+GL LS  +M  AA++E KR+D   +S  +   L+  W+  Q+L  G +++FT  G
Sbjct: 447 TRIGIGLFLSTFSMVSAAMLEKKRRD---SSVLDGRILSIFWITPQFLIFGISEMFTAVG 506

Query: 488 LLEFFFTEAPTGMRSLATSLSWASLAVGYYLSSVIVTVVNGVTARSGHTP-WLSGDNINH 547
           L+EFF+ ++  GM S   +L++ S + G+Y SSV+V+VVN +T+ S  +  WL  +++N 
Sbjct: 507 LIEFFYKQSAKGMESFLMALTYCSYSFGFYFSSVLVSVVNKITSTSVDSKGWLGENDLNK 566

Query: 548 YHLDRFYWLMCTLTGLNFLHYLFWA 566
             LD FYWL+  L+ LNFL YLFW+
Sbjct: 567 DRLDLFYWLLAVLSLLNFLSYLFWS 571

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8H1572.2e-22569.98Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1[more]
Q8VYE42.5e-20564.97Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1[more]
Q9FM207.5e-18659.24Protein NRT1/ PTR FAMILY 4.7 OS=Arabidopsis thaliana OX=3702 GN=NPF4.7 PE=2 SV=2[more]
Q93VV57.0e-10741.42Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1[more]
Q9LSE82.3e-10239.22Protein NRT1/ PTR FAMILY 4.2 OS=Arabidopsis thaliana OX=3702 GN=NPF4.2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022158071.10.0100.00protein NRT1/ PTR FAMILY 4.6-like [Momordica charantia][more]
XP_038886228.11.66e-31378.03protein NRT1/ PTR FAMILY 4.6-like [Benincasa hispida][more]
XP_011657481.17.25e-30676.25protein NRT1/ PTR FAMILY 4.6 [Cucumis sativus] >APY23675.1 NRT1.2-like nitrate t... [more]
XP_008456458.11.90e-30376.72PREDICTED: protein NRT1/ PTR FAMILY 4.6-like [Cucumis melo] >KAA0036440.1 protei... [more]
KAB1220739.17.37e-29173.14Protein NRT1/ PTR FAMILY 4.6 [Morella rubra][more]
Match NameE-valueIdentityDescription
A0A6J1DZY80.0100.00protein NRT1/ PTR FAMILY 4.6-like OS=Momordica charantia OX=3673 GN=LOC111024644... [more]
A0A1P8SN463.51e-30676.25NRT1.2-like nitrate transporter OS=Cucumis sativus OX=3659 GN=NRT1.2C PE=2 SV=1[more]
A0A5D3BLL09.19e-30476.72Protein NRT1/ PTR FAMILY 4.6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3C3A19.19e-30476.72protein NRT1/ PTR FAMILY 4.6-like OS=Cucumis melo OX=3656 GN=LOC103496401 PE=3 S... [more]
A0A6A1W6473.57e-29173.14Protein NRT1/ PTR FAMILY 4.6 OS=Morella rubra OX=262757 GN=CJ030_MR3G002950 PE=3... [more]
Match NameE-valueIdentityDescription
AT1G69850.11.5e-22669.98nitrate transporter 1:2 [more]
AT1G27040.21.8e-20664.97Major facilitator superfamily protein [more]
AT1G27040.11.8e-20664.97Major facilitator superfamily protein [more]
AT5G62730.15.3e-18759.24Major facilitator superfamily protein [more]
AT1G59740.14.9e-10841.42Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 95..532
e-value: 1.9E-105
score: 352.9
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 10..574
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 1..574
e-value: 1.4E-170
score: 570.0
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 44..571
NoneNo IPR availablePANTHERPTHR11654:SF490PROTEIN NRT1/ PTR FAMILY 4.6-LIKEcoord: 10..574
NoneNo IPR availableCDDcd17414MFS_NPF4coord: 26..565
e-value: 0.0
score: 633.292

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g1553.1MC08g1553.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006817 phosphate ion transport
biological_process GO:0050896 response to stimulus
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity