MC08g1412 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g1412
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	ribonuclease MC-like
Location	MC08: 15784818 .. 15786172 (+)
RNA-Seq Expression	MC08g1412
Synteny	MC08g1412

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

AAACAACTTTGTTTGAGCAAAGAATTGTGGCCAAAGGATCCAATGAATCTCTTCGTGAAGCGTGAAACTTGAGAGGGTGCTACTACACCTATTTTCGACTTGTACTAATTATTTGAGTATCGAAGTGCTCACTCTCTGTCTTGCATGTTTCACATGTTTCACTATTCACGAGGATATTCATTCAATCGTTTGGACAGATATTCTATGCTCGAACAAACATAATTTACCGTTTATGGTGATTCAACCATTGTGATGCATGTGAAAAAAAGTTGGTATCATAATGCTATAAATAGCACTAGTTCAGTAGATGCGTGATACCAATTGAGATCTCACAACCAAAGGTACCAAAGAAAAAGAAATGGGAAAGAGAGAAATTTTATTGGTTTTGAGTATGTTTCTAGCGATTTCAAGCTCAGAGGGCGTGAACGAATTCGATTACTACCAAGTCGTGCTTCAATGGCAACCGGCCACTTGTAGCAGTGCAACTCTAAAAACTGTATGCGTGCAACCTCCAGAAGATAGATATAGTGTCCATGGTGTTTGGCCTAGCCTATCGTCAGGATATCTTCCAACGTGTTCTGGTACTCCGCCCTATGATGCCAGAATGGTAGTCGTTCATATATCTTATAAATCTCGTTAGGTCCCCTAATCTTTTTAACGTGGGATGTTTATCAATCTAAATGTTTTTGTTTTGAAATTCAGATATTGACGATTCAAGCTAGGTTAGACCAAGACTGGCCAAACGTGATAACCGGACAAAACCCGCAGTTTTGGAGCTACGAGTGGACAAAGCACGGGACTTGTTCGAGCGCGTATCTGAACCAATTCCACTACTTCAATTTTGGACTATGGGTATATACCAATTACGATCTGCAATCTATTCTGTATCAATATGGTATCTACCCCAATGGACAACAATACACAAAAACAGTTGTCCAAAATGCAATTTATAGTGCAACGGGAAAGTGGCCAGCCCTTCGCTGCAACAACAACTACAGAACTGGTAACATGCAGTTGCATGAAATAAGTTTGTGTTTCAGCAAGAATGGACAGATTCTCATAGACTGCCCATTCAATGTCGTTACATGCAATGAATATTTCGTATGGAATTCATGGAGTTATTCAGCGAGTGTGGCTAAGGAGTAAAACTAATCACTGCCTTGAAACTGCTCTTACTACGAGGGTTAAGCTGTTGGAAGTTATGTTTCTTAATAAATATCTATCTTTAGTGTGTTTGTAAGATATGTTTTCTAATAATTGTCTACCTTCATCGTGTGTTTATGAGGGATAGATGGAACAAGTTGGTGTGTAATGCCGAAAGAAAGAACAATCTAATGAATAAGTTTATATCTTGT

mRNA sequence

AAACAACTTTGTTTGAGCAAAGAATTGTGGCCAAAGGATCCAATGAATCTCTTCGTGAAGCGTGAAACTTGAGAGGGTGCTACTACACCTATTTTCGACTTGTACTAATTATTTGAGTATCGAAGTGCTCACTCTCTGTCTTGCATGTTTCACATGTTTCACTATTCACGAGGATATTCATTCAATCGTTTGGACAGATATTCTATGCTCGAACAAACATAATTTACCGTTTATGGTGATTCAACCATTGTGATGCATGTGAAAAAAAGTTGGTATCATAATGCTATAAATAGCACTAGTTCAGTAGATGCGTGATACCAATTGAGATCTCACAACCAAAGGTACCAAAGAAAAAGAAATGGGAAAGAGAGAAATTTTATTGGTTTTGAGTATGTTTCTAGCGATTTCAAGCTCAGAGGGCGTGAACGAATTCGATTACTACCAAGTCGTGCTTCAATGGCAACCGGCCACTTGTAGCAGTGCAACTCTAAAAACTGTATGCGTGCAACCTCCAGAAGATAGATATAGTGTCCATGGTGTTTGGCCTAGCCTATCGTCAGGATATCTTCCAACGTGTTCTGGTACTCCGCCCTATGATGCCAGAATGATATTGACGATTCAAGCTAGGTTAGACCAAGACTGGCCAAACGTGATAACCGGACAAAACCCGCAGTTTTGGAGCTACGAGTGGACAAAGCACGGGACTTGTTCGAGCGCGTATCTGAACCAATTCCACTACTTCAATTTTGGACTATGGGTATATACCAATTACGATCTGCAATCTATTCTGTATCAATATGGTATCTACCCCAATGGACAACAATACACAAAAACAGTTGTCCAAAATGCAATTTATAGTGCAACGGGAAAGTGGCCAGCCCTTCGCTGCAACAACAACTACAGAACTGGTAACATGCAGTTGCATGAAATAAGTTTGTGTTTCAGCAAGAATGGACAGATTCTCATAGACTGCCCATTCAATGTCGTTACATGCAATGAATATTTCGTATGGAATTCATGGAGTTATTCAGCGAGTGTGGCTAAGGAGTAAAACTAATCACTGCCTTGAAACTGCTCTTACTACGAGGGTTAAGCTGTTGGAAGTTATGTTTCTTAATAAATATCTATCTTTAGTGTGTTTGTAAGATATGTTTTCTAATAATTGTCTACCTTCATCGTGTGTTTATGAGGGATAGATGGAACAAGTTGGTGTGTAATGCCGAAAGAAAGAACAATCTAATGAATAAGTTTATATCTTGT

Coding sequence (CDS)

ATGGGAAAGAGAGAAATTTTATTGGTTTTGAGTATGTTTCTAGCGATTTCAAGCTCAGAGGGCGTGAACGAATTCGATTACTACCAAGTCGTGCTTCAATGGCAACCGGCCACTTGTAGCAGTGCAACTCTAAAAACTGTATGCGTGCAACCTCCAGAAGATAGATATAGTGTCCATGGTGTTTGGCCTAGCCTATCGTCAGGATATCTTCCAACGTGTTCTGGTACTCCGCCCTATGATGCCAGAATGATATTGACGATTCAAGCTAGGTTAGACCAAGACTGGCCAAACGTGATAACCGGACAAAACCCGCAGTTTTGGAGCTACGAGTGGACAAAGCACGGGACTTGTTCGAGCGCGTATCTGAACCAATTCCACTACTTCAATTTTGGACTATGGGTATATACCAATTACGATCTGCAATCTATTCTGTATCAATATGGTATCTACCCCAATGGACAACAATACACAAAAACAGTTGTCCAAAATGCAATTTATAGTGCAACGGGAAAGTGGCCAGCCCTTCGCTGCAACAACAACTACAGAACTGGTAACATGCAGTTGCATGAAATAAGTTTGTGTTTCAGCAAGAATGGACAGATTCTCATAGACTGCCCATTCAATGTCGTTACATGCAATGAATATTTCGTATGGAATTCATGGAGTTATTCAGCGAGTGTGGCTAAGGAGTAA

Protein sequence

MGKREILLVLSMFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHGVWPSLSSGYLPTCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSSAYLNQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNNYRTGNMQLHEISLCFSKNGQILIDCPFNVVTCNEYFVWNSWSYSASVAKE

Homology

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BLAST of MC08g1412 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 3.8e-32
Identity = 69/194 (35.57%), Postives = 105/194 (54.12%), Query Frame = 0


Query: 25  FDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHGVWPSLSSGYLPTCSGTPPYDARMI 84
           FD +  V QW PA CS     + C       +++HG+WP  S   L  C    P+D   I
Sbjct: 1   FDSFWFVQQWPPAVCSFQKSGS-CPGSGLRTFTIHGLWPQGSGTSLTNCPQGSPFDITKI 60

Query: 85  LTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSSAYLNQFHYFNFGLWVYTNYDLQSIL 144
             +Q++L+  WPNV+   N QFWS+EWTKHGTCS +  NQ  YF   + +  NYD+   L
Sbjct: 61  SHLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGAL 120

Query: 145 YQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNNYRTGNMQLHEISLCFSKNGQILID 204
             +   PNG+  ++  ++  + +  GK+P LRC  + +T    L ++  CF+++G  LID
Sbjct: 121 RPHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQVVACFAQDGSTLID 180

Query: 205 CPFNVVTCNEYFVW 219
           C  +  TC   F++
Sbjct: 181 CTRD--TCGANFIF 191

[Close]

BLAST of MC08g1412 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 2.6e-28
Identity = 67/207 (32.37%), Postives = 113/207 (54.59%), Query Frame = 0


Query: 6   ILLVLSMFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQ---PPEDRYSVHGVW 65
           ++++ S+F A SSSE   +FD++  V QW  + C   T K  C      P   + +HG+W
Sbjct: 14  LVILPSVFSASSSSE---DFDFFYFVQQWPGSYCD--TQKKCCYPNSGKPAADFGIHGLW 73

Query: 66  PSLSSGYLPT-CSGTPPYDARMILTIQARLDQDWPNVI--TGQNPQFWSYEWTKHGTCSS 125
           P+   G  P+ C  + P+D+  I  +   + + WP +   +G    FW +EW KHGTCS 
Sbjct: 74  PNYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSE 133

Query: 126 AYLNQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNN 185
           + ++Q  YF   L +    +L   L + GI P+G+ Y+   ++++I  + G  P + CN 
Sbjct: 134 SVIDQHEYFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECNR 193

Query: 186 NYRTGNMQLHEISLCFSKNGQILIDCP 207
           +  +GN QL+++ LC  ++G  LI+CP
Sbjct: 194 D-GSGNSQLYQVYLCVDRSGSGLIECP 214

[Close]

BLAST of MC08g1412 vs. ExPASy Swiss-Prot
Match: Q7M438 (Ribonuclease DdI OS=Dictyostelium discoideum OX=44689 GN=ddiA PE=1 SV=3)

HSP 1 Score: 119.0 bits (297), Expect = 7.0e-26
Identity = 69/216 (31.94%), Postives = 116/216 (53.70%), Query Frame = 0


Query: 4   REILLVLSMFLAISSSEGV---------NEFDYYQVVLQWQPATCSSATLKTVCVQPPE- 63
           R I  +LS+ L  S+++            +FD+Y  V QW  + C S T    C+Q  E 
Sbjct: 2   RLIAALLSVLLIASTAQSTVTIYESSKPGDFDFYLFVQQWIYSYCDSQT----CIQNKER 61

Query: 64  DRYSVHGVWPSLSSGYLPTCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTK 123
           + +++HG+WP  S G  P+    P ++   I  ++ +L+ DWP+ +TG N  FW++E++K
Sbjct: 62  EAFTIHGLWPENSDGSYPSFCSGPSFNVNAIQDLEDQLNFDWPS-LTGPNTDFWTHEFSK 121

Query: 124 HGTCS-SAYLNQFH-YFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTV-VQNAIYSATG 183
           HGTCS +  +   H YF  G+ +YT +++ + L    IYP+     K V + NAI +  G
Sbjct: 122 HGTCSITGPITDIHDYFATGIKLYTEFNITAALESENIYPSDSNTYKPVDITNAITTHFG 181

Query: 184 KWPALRCNNNYRTGNMQLHEISLCFSKNGQILIDCP 207
             P ++C++       QL  +++C  KN   ++DCP
Sbjct: 182 GKPGIQCSSG------QLSTVAVCIDKNSLSIMDCP 206

[Close]

BLAST of MC08g1412 vs. ExPASy Swiss-Prot
Match: Q40966 (Ribonuclease S-4 OS=Pyrus pyrifolia OX=3767 PE=1 SV=2)

HSP 1 Score: 112.8 bits (281), Expect = 5.0e-24
Identity = 70/214 (32.71%), Postives = 111/214 (51.87%), Query Frame = 0


Query: 8   LVLSMFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHGVWPSLSS 67
           +VLS+ + I S+  V  FDY+Q   Q+QPA C+S    T C  P +  ++VHG+WPS  +
Sbjct: 12  MVLSLIVLIFSASTVG-FDYFQFTQQYQPAVCNSN--PTPCNDPTDKLFTVHGLWPSNRN 71

Query: 68  GYLPTCSGTPPYDARMILTIQARLDQDWPNVIT-GQNPQFWSYEWTKHGTCSSAYL-NQF 127
           G  P    T   +++ I  + A+L+  WPNV+    +  FW  EW KHGTC    + +  
Sbjct: 72  GPDPEKCKTTTMNSQKIGNMTAQLEIIWPNVLNRSDHVGFWEREWLKHGTCGYPTIKDDM 131

Query: 128 HYFN--FGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKW-PALRCNNNYR 187
           HY      +++    ++ +IL +  I PNG   +   ++NAI S      P  +C  N R
Sbjct: 132 HYLKTVIKMYITQKQNVSAILSKATIQPNGNNRSLVDIENAIRSGNNNTKPKFKCQKNTR 191

Query: 188 TGNMQLHEISLCFSKNGQILIDCPFNVVTCNEYF 217
           T   +L E++LC +++    I+CP      + YF
Sbjct: 192 T-TTELVEVTLCSNRDLTKFINCPHGPPKGSRYF 221

[Close]

BLAST of MC08g1412 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.1e-23
Identity = 60/190 (31.58%), Postives = 96/190 (50.53%), Query Frame = 0


Query: 24  EFDYYQVVLQWQPATCSSATLKTVCVQP----PEDRYSVHGVWPSLSSGYLP-TCSGTPP 83
           +FD++  VLQW  A C S   +  C  P    P   + +HG+WP+  +G  P  C+    
Sbjct: 21  DFDFFYFVLQWPGAYCDS---RHSCCYPQTGKPAADFGIHGLWPNYKTGGWPQNCNPDSR 80

Query: 84  YDARMILTIQARLDQDWPNVITGQNP--QFWSYEWTKHGTCSSAYLNQFHYFNFGLWVYT 143
           +D   +  + + L ++WP +    N   +FW++EW KHGTC+ + L+Q  YF  GL +  
Sbjct: 81  FDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESELDQHDYFEAGLKLKQ 140

Query: 144 NYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNNYRTGNMQLHEISLCFS 203
             +L   L   GI P+ + Y    ++N I    G  P + CN +  + N QL++I LC  
Sbjct: 141 KANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNKD-SSHNSQLYQIYLCVD 200

Query: 204 KNGQILIDCP 207
            +    I+CP
Sbjct: 201 TSASKFINCP 206

[Close]

BLAST of MC08g1412 vs. NCBI nr
Match: XP_022158939.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 491 bits (1263), Expect = 1.90e-175
Identity = 230/230 (100.00%), Postives = 230/230 (100.00%), Query Frame = 0


Query: 1   MGKREILLVLSMFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHG 60
           MGKREILLVLSMFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHG
Sbjct: 1   MGKREILLVLSMFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHG 60

Query: 61  VWPSLSSGYLPTCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSSA 120
           VWPSLSSGYLPTCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSSA
Sbjct: 61  VWPSLSSGYLPTCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSSA 120

Query: 121 YLNQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNN 180
           YLNQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNN
Sbjct: 121 YLNQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNN 180

Query: 181 YRTGNMQLHEISLCFSKNGQILIDCPFNVVTCNEYFVWNSWSYSASVAKE 230
           YRTGNMQLHEISLCFSKNGQILIDCPFNVVTCNEYFVWNSWSYSASVAKE
Sbjct: 181 YRTGNMQLHEISLCFSKNGQILIDCPFNVVTCNEYFVWNSWSYSASVAKE 230

[Close]

BLAST of MC08g1412 vs. NCBI nr
Match: XP_022156540.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 319 bits (817), Expect = 1.86e-107
Identity = 151/227 (66.52%), Postives = 180/227 (79.30%), Query Frame = 0


Query: 1   MGKR-EILLVLSMFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVH 60
           M KR EI+L+LSM L I  SEGVNEFDYYQVVLQWQPATCSS+TL   CVQPP++R+S+H
Sbjct: 1   MAKRSEIVLILSMLLLIGWSEGVNEFDYYQVVLQWQPATCSSSTLVDECVQPPDERFSIH 60

Query: 61  GVWPSLSSGYLPTCSGTPPYDARMI-LTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCS 120
           GVWPSL SG    CSGTPPYD   +  +I+ARL QDWP+V+   +P FWS+EWT HGTCS
Sbjct: 61  GVWPSLLSGDA-VCSGTPPYDGTQLNSSIRARLHQDWPSVVHRVDPPFWSHEWTLHGTCS 120

Query: 121 SAYLNQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCN 180
            AYL+QFHYF+FGL+VY NYD+  IL  Y +YPNG  Y ++ V+N IYSATGKWPALRCN
Sbjct: 121 YAYLDQFHYFDFGLFVYGNYDVLGILNYYNVYPNGNLYARSDVENIIYSATGKWPALRCN 180

Query: 181 NNYRTGNMQLHEISLCFSKNGQILIDCPFNVVTCNEYFVWNSWSYSA 225
           NN+ +G++QLHEISLCF K    L DCP+N V CN+YFVWNSWS +A
Sbjct: 181 NNFWSGDVQLHEISLCFRKQDFSLEDCPYNAVKCNDYFVWNSWSSNA 226

[Close]

BLAST of MC08g1412 vs. NCBI nr
Match: XP_038903348.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 293 bits (749), Expect = 2.82e-97
Identity = 133/221 (60.18%), Postives = 171/221 (77.38%), Query Frame = 0


Query: 1   MGKREILLVLS-MFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVH 60
           MGKREI+LVLS + L + SS+G+NEFDYYQV+ QWQPA CSSATLK+ CV+ PE+R+++H
Sbjct: 1   MGKREIMLVLSILLLLVGSSKGLNEFDYYQVIFQWQPAACSSATLKSPCVKRPENRFTIH 60

Query: 61  GVWPSLSSGYLPTCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSS 120
           GVWPSL+SG    CSGTPPYD   I  I++RLDQDWP+V+ G NP  WS+EW  HGTCS+
Sbjct: 61  GVWPSLTSGNATKCSGTPPYDGNKIYAIRSRLDQDWPSVVYGTNPTLWSHEWKTHGTCSN 120

Query: 121 AYLNQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNN 180
            YL+Q++YFNFGL V+  Y++ SIL +YGI P+G  Y+   +++AIY+AT KWPALRCN 
Sbjct: 121 YYLDQYNYFNFGLSVFGKYNMISILRKYGINPDGNDYSIDYIESAIYNATVKWPALRCNK 180

Query: 181 NYRTGNMQLHEISLCFSKNGQILIDCPFNVVTCNEYFVWNS 220
           N  +G  QL+EI+LCF+K  + L DCP+N VTC   F WNS
Sbjct: 181 NKSSGKKQLYEIALCFTKVNRALQDCPYNAVTCGNKFSWNS 221

[Close]

BLAST of MC08g1412 vs. NCBI nr
Match: XP_022159354.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 248 bits (632), Expect = 1.94e-79
Identity = 121/230 (52.61%), Postives = 150/230 (65.22%), Query Frame = 0


Query: 1   MGKREILLVLSMFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHG 60
           M K+ I+LV S  + I+S +    ++YYQV LQWQPATCS A L+T CV  P+DR+S+HG
Sbjct: 1   MEKKGIVLVFSTLVVIASCD----YNYYQVTLQWQPATCSDANLQTPCVNTPQDRFSIHG 60

Query: 61  VWPSLSSGYLPTCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSSA 120
           VWP+LSSG  P C GTP YD   I TI+ RL+ DWP+VI G NP  WS+EWT HGTCS  
Sbjct: 61  VWPTLSSGSYPQCPGTPAYDGSKISTIRNRLNADWPSVIYGTNPTLWSHEWTTHGTCSYP 120

Query: 121 YLNQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNN 180
            L+Q  YF FGL  Y  YD+ S L +  IYP+   Y+K+ + NAI   T K P LRCN N
Sbjct: 121 SLSQLDYFTFGLDTYAKYDIYSALDKEDIYPSEDVYSKSSINNAIRKITQKLPGLRCNTN 180

Query: 181 YRTGNMQLHEISLCFSKNGQILIDCPFNVVTCNEYFVWNSWSYSASVAKE 230
             +   QLHEI LCF+K+ + +IDCP NVVTC  Y  W  W Y   V K+
Sbjct: 181 DYSRERQLHEIILCFAKSNRAIIDCPSNVVTCGNYVYWYPW-YPEGVKKQ 225

[Close]

BLAST of MC08g1412 vs. NCBI nr
Match: AEM42996.1 (S-RNase [Siraitia grosvenorii])

HSP 1 Score: 235 bits (600), Expect = 1.69e-74
Identity = 114/231 (49.35%), Postives = 155/231 (67.10%), Query Frame = 0


Query: 1   MGKREILLVLSMFLAISSSEGVNEFDYYQVVLQWQPATCSSATLK-TVCVQPPEDRYSVH 60
           M KREI+LVLSM + I+S EG+NEFDY QVV+QWQPATCS++     +C Q P++RYS+H
Sbjct: 1   MAKREIVLVLSMLVLIASCEGLNEFDYLQVVMQWQPATCSASNKPYPICYQNPDNRYSIH 60

Query: 61  GVWPSLSSGYLPTCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSS 120
           GVWPSL SG    CSG+P ++A  I  +Q  + + WPN+I G N   W +EW KHGTC+ 
Sbjct: 61  GVWPSLYSGAATQCSGSP-FNANAISILQPGISEIWPNIINGNNLWLWGHEWDKHGTCTE 120

Query: 121 AYL-NQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCN 180
             L +Q  YF+F +  Y +Y+L  +L    I PNGQ Y++  V  AI + TGK PA+RCN
Sbjct: 121 EILFDQDRYFSFAMDTYIDYNLLVLLGNSQITPNGQIYSRDQVYAAIRAKTGKTPAVRCN 180

Query: 181 NNYRTGNMQLHEISLCFSKNGQILIDCPFNVVTCNEYFVWNSWSYSASVAK 229
            N  TG  Q+HE+SLC++ +   ++DCP N   C+ YFVW SWS   +V++
Sbjct: 181 YNRWTGEQQMHEVSLCYNHDASAVVDCPLNANKCDNYFVWYSWSTQKAVSE 230

[Close]

BLAST of MC08g1412 vs. ExPASy TrEMBL
Match: A0A6J1E0X1 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025395 PE=3 SV=1)

HSP 1 Score: 491 bits (1263), Expect = 9.19e-176
Identity = 230/230 (100.00%), Postives = 230/230 (100.00%), Query Frame = 0


Query: 1   MGKREILLVLSMFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHG 60
           MGKREILLVLSMFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHG
Sbjct: 1   MGKREILLVLSMFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHG 60

Query: 61  VWPSLSSGYLPTCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSSA 120
           VWPSLSSGYLPTCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSSA
Sbjct: 61  VWPSLSSGYLPTCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSSA 120

Query: 121 YLNQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNN 180
           YLNQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNN
Sbjct: 121 YLNQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNN 180

Query: 181 YRTGNMQLHEISLCFSKNGQILIDCPFNVVTCNEYFVWNSWSYSASVAKE 230
           YRTGNMQLHEISLCFSKNGQILIDCPFNVVTCNEYFVWNSWSYSASVAKE
Sbjct: 181 YRTGNMQLHEISLCFSKNGQILIDCPFNVVTCNEYFVWNSWSYSASVAKE 230

[Close]

BLAST of MC08g1412 vs. ExPASy TrEMBL
Match: A0A6J1DQK2 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111023418 PE=3 SV=1)

HSP 1 Score: 319 bits (817), Expect = 9.00e-108
Identity = 151/227 (66.52%), Postives = 180/227 (79.30%), Query Frame = 0


Query: 1   MGKR-EILLVLSMFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVH 60
           M KR EI+L+LSM L I  SEGVNEFDYYQVVLQWQPATCSS+TL   CVQPP++R+S+H
Sbjct: 1   MAKRSEIVLILSMLLLIGWSEGVNEFDYYQVVLQWQPATCSSSTLVDECVQPPDERFSIH 60

Query: 61  GVWPSLSSGYLPTCSGTPPYDARMI-LTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCS 120
           GVWPSL SG    CSGTPPYD   +  +I+ARL QDWP+V+   +P FWS+EWT HGTCS
Sbjct: 61  GVWPSLLSGDA-VCSGTPPYDGTQLNSSIRARLHQDWPSVVHRVDPPFWSHEWTLHGTCS 120

Query: 121 SAYLNQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCN 180
            AYL+QFHYF+FGL+VY NYD+  IL  Y +YPNG  Y ++ V+N IYSATGKWPALRCN
Sbjct: 121 YAYLDQFHYFDFGLFVYGNYDVLGILNYYNVYPNGNLYARSDVENIIYSATGKWPALRCN 180

Query: 181 NNYRTGNMQLHEISLCFSKNGQILIDCPFNVVTCNEYFVWNSWSYSA 225
           NN+ +G++QLHEISLCF K    L DCP+N V CN+YFVWNSWS +A
Sbjct: 181 NNFWSGDVQLHEISLCFRKQDFSLEDCPYNAVKCNDYFVWNSWSSNA 226

[Close]

BLAST of MC08g1412 vs. ExPASy TrEMBL
Match: A0A6J1E266 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025771 PE=3 SV=1)

HSP 1 Score: 248 bits (632), Expect = 9.38e-80
Identity = 121/230 (52.61%), Postives = 150/230 (65.22%), Query Frame = 0


Query: 1   MGKREILLVLSMFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHG 60
           M K+ I+LV S  + I+S +    ++YYQV LQWQPATCS A L+T CV  P+DR+S+HG
Sbjct: 1   MEKKGIVLVFSTLVVIASCD----YNYYQVTLQWQPATCSDANLQTPCVNTPQDRFSIHG 60

Query: 61  VWPSLSSGYLPTCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSSA 120
           VWP+LSSG  P C GTP YD   I TI+ RL+ DWP+VI G NP  WS+EWT HGTCS  
Sbjct: 61  VWPTLSSGSYPQCPGTPAYDGSKISTIRNRLNADWPSVIYGTNPTLWSHEWTTHGTCSYP 120

Query: 121 YLNQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNN 180
            L+Q  YF FGL  Y  YD+ S L +  IYP+   Y+K+ + NAI   T K P LRCN N
Sbjct: 121 SLSQLDYFTFGLDTYAKYDIYSALDKEDIYPSEDVYSKSSINNAIRKITQKLPGLRCNTN 180

Query: 181 YRTGNMQLHEISLCFSKNGQILIDCPFNVVTCNEYFVWNSWSYSASVAKE 230
             +   QLHEI LCF+K+ + +IDCP NVVTC  Y  W  W Y   V K+
Sbjct: 181 DYSRERQLHEIILCFAKSNRAIIDCPSNVVTCGNYVYWYPW-YPEGVKKQ 225

[Close]

BLAST of MC08g1412 vs. ExPASy TrEMBL
Match: K7NBR4 (S-RNase OS=Siraitia grosvenorii OX=190515 PE=2 SV=1)

HSP 1 Score: 235 bits (600), Expect = 8.19e-75
Identity = 114/231 (49.35%), Postives = 155/231 (67.10%), Query Frame = 0


Query: 1   MGKREILLVLSMFLAISSSEGVNEFDYYQVVLQWQPATCSSATLK-TVCVQPPEDRYSVH 60
           M KREI+LVLSM + I+S EG+NEFDY QVV+QWQPATCS++     +C Q P++RYS+H
Sbjct: 1   MAKREIVLVLSMLVLIASCEGLNEFDYLQVVMQWQPATCSASNKPYPICYQNPDNRYSIH 60

Query: 61  GVWPSLSSGYLPTCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSS 120
           GVWPSL SG    CSG+P ++A  I  +Q  + + WPN+I G N   W +EW KHGTC+ 
Sbjct: 61  GVWPSLYSGAATQCSGSP-FNANAISILQPGISEIWPNIINGNNLWLWGHEWDKHGTCTE 120

Query: 121 AYL-NQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCN 180
             L +Q  YF+F +  Y +Y+L  +L    I PNGQ Y++  V  AI + TGK PA+RCN
Sbjct: 121 EILFDQDRYFSFAMDTYIDYNLLVLLGNSQITPNGQIYSRDQVYAAIRAKTGKTPAVRCN 180

Query: 181 NNYRTGNMQLHEISLCFSKNGQILIDCPFNVVTCNEYFVWNSWSYSASVAK 229
            N  TG  Q+HE+SLC++ +   ++DCP N   C+ YFVW SWS   +V++
Sbjct: 181 YNRWTGEQQMHEVSLCYNHDASAVVDCPLNANKCDNYFVWYSWSTQKAVSE 230

[Close]

BLAST of MC08g1412 vs. ExPASy TrEMBL
Match: A0A6J1D7Q5 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111018138 PE=3 SV=1)

HSP 1 Score: 235 bits (599), Expect = 1.12e-74
Identity = 119/232 (51.29%), Postives = 151/232 (65.09%), Query Frame = 0


Query: 1   MGKREIL-LVLSMFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVH 60
           M +REI+ LVLSM + ++  EG+NEFDY QVV+QWQPATCS    K  C Q P +R+S+H
Sbjct: 1   MAQREIIVLVLSMLVLVAICEGLNEFDYLQVVIQWQPATCSDYN-KPACFQAPGNRFSIH 60

Query: 61  GVWPSLSSGYLPTCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCS- 120
           GVWPSL  GY   CSG+P +D   I  +Q  + + WPNV TG N   W +EW KHGTCS 
Sbjct: 61  GVWPSLYDGYPTQCSGSP-FDRNQISGLQPGISEIWPNVATGDNFWLWQHEWDKHGTCSE 120

Query: 121 SAYLNQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCN 180
           S + +Q  YF FG+  Y + +LQ IL    I PNG+ YT+  V  AI + TGK PA+RCN
Sbjct: 121 STFYDQQEYFGFGMDAYIHNNLQVILSNNQITPNGRTYTRDQVYAAIRAKTGKTPAVRCN 180

Query: 181 NNYRTGNMQLHEISLCFSKNGQILIDCPFNVVTCNEYFVWNSWSYSASVAKE 230
            N RTG  QLHEISLCF K+  +  DCP N   C   FVWNSW+   ++++E
Sbjct: 181 YNSRTGASQLHEISLCFKKDASVFEDCPLNANKCGASFVWNSWNTVKAISEE 230

[Close]

BLAST of MC08g1412 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 127.1 bits (318), Expect = 1.8e-29
Identity = 67/207 (32.37%), Postives = 113/207 (54.59%), Query Frame = 0


Query: 6   ILLVLSMFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQ---PPEDRYSVHGVW 65
           ++++ S+F A SSSE   +FD++  V QW  + C   T K  C      P   + +HG+W
Sbjct: 14  LVILPSVFSASSSSE---DFDFFYFVQQWPGSYCD--TQKKCCYPNSGKPAADFGIHGLW 73

Query: 66  PSLSSGYLPT-CSGTPPYDARMILTIQARLDQDWPNVI--TGQNPQFWSYEWTKHGTCSS 125
           P+   G  P+ C  + P+D+  I  +   + + WP +   +G    FW +EW KHGTCS 
Sbjct: 74  PNYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSE 133

Query: 126 AYLNQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNN 185
           + ++Q  YF   L +    +L   L + GI P+G+ Y+   ++++I  + G  P + CN 
Sbjct: 134 SVIDQHEYFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECNR 193

Query: 186 NYRTGNMQLHEISLCFSKNGQILIDCP 207
           +  +GN QL+++ LC  ++G  LI+CP
Sbjct: 194 D-GSGNSQLYQVYLCVDRSGSGLIECP 214

[Close]

BLAST of MC08g1412 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 120.2 bits (300), Expect = 2.2e-27
Identity = 67/208 (32.21%), Postives = 101/208 (48.56%), Query Frame = 0


Query: 6   ILLVLSMFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQP----PEDRYSVHGV 65
           +LL L +F  +  S    +FD++  VLQW  A C ++     C  P    P   + +HG+
Sbjct: 9   VLLKLLVFQGLFVSRPQEDFDFFYFVLQWPGAYCDTS---RACCYPTSGKPAADFGIHGL 68

Query: 66  WPSLSSGYLPT-CSGTPPYDARMILTIQARLDQDWPNVITGQNP--QFWSYEWTKHGTCS 125
           WP+ + G  P+ C     +D   I  + + L ++WP +    N    FW +EW KHGTCS
Sbjct: 69  WPNYNGGSWPSNCDPDSQFDRSQISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEKHGTCS 128

Query: 126 SAYLNQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCN 185
            + ++Q  YF   L +    +L  IL   GI P+   Y    + NAI    G  P + CN
Sbjct: 129 ESVMDQHEYFENALKLKQKANLLQILKNSGINPDDGFYNLDKITNAIKDGIGFTPGIECN 188

Query: 186 NNYRTGNMQLHEISLCFSKNGQILIDCP 207
            +    N QLH+I +C   +G   I+CP
Sbjct: 189 KDPER-NAQLHQIYICVDTSGTEFIECP 212

[Close]

BLAST of MC08g1412 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 111.7 bits (278), Expect = 7.9e-25
Identity = 60/190 (31.58%), Postives = 96/190 (50.53%), Query Frame = 0


Query: 24  EFDYYQVVLQWQPATCSSATLKTVCVQP----PEDRYSVHGVWPSLSSGYLP-TCSGTPP 83
           +FD++  VLQW  A C S   +  C  P    P   + +HG+WP+  +G  P  C+    
Sbjct: 21  DFDFFYFVLQWPGAYCDS---RHSCCYPQTGKPAADFGIHGLWPNYKTGGWPQNCNPDSR 80

Query: 84  YDARMILTIQARLDQDWPNVITGQNP--QFWSYEWTKHGTCSSAYLNQFHYFNFGLWVYT 143
           +D   +  + + L ++WP +    N   +FW++EW KHGTC+ + L+Q  YF  GL +  
Sbjct: 81  FDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESELDQHDYFEAGLKLKQ 140

Query: 144 NYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNNYRTGNMQLHEISLCFS 203
             +L   L   GI P+ + Y    ++N I    G  P + CN +  + N QL++I LC  
Sbjct: 141 KANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNKD-SSHNSQLYQIYLCVD 200

Query: 204 KNGQILIDCP 207
            +    I+CP
Sbjct: 201 TSASKFINCP 206

[Close]

BLAST of MC08g1412 vs. TAIR 10
Match: AT1G14210.1 (Ribonuclease T2 family protein )

HSP 1 Score: 107.8 bits (268), Expect = 1.1e-23
Identity = 61/211 (28.91%), Postives = 99/211 (46.92%), Query Frame = 0


Query: 6   ILLVLSMFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDR----YSVHGV 65
           + L++   L +SSS+   +F+++  V  W  A C S   +  C  P +      + +HG+
Sbjct: 7   VSLLILQSLVVSSSQTEPDFNFFYWVNYWPGAICDS---QKGCCPPTKGNTASDFIIHGL 66

Query: 66  WPSLSSGYLPT-CSGTPPYDARMILTIQARLDQDWPN----VITGQNPQFWSYEWTKHGT 125
           WP  ++G  P  C  T  +D   I  +  ++++ W               W +EW KHGT
Sbjct: 67  WPQFNNGTWPAFCDQTNLFDISKISDLVCQMEKKWTEWGVWACPSNETNLWEHEWNKHGT 126

Query: 126 CSSAYLNQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALR 185
           C  +  +Q  YF   L       L +IL Q GI PN   Y+   ++NAI  A G  P + 
Sbjct: 127 CVQSIFDQHSYFRTNLKFKHKVHLLNILIQKGIKPNDGFYSLDEIKNAIKCAIGFAPGIE 186

Query: 186 CNNNYRTGNMQLHEISLCFSKNGQILIDCPF 208
           CN + + GN QL +I +C     +  ++CP+
Sbjct: 187 CNEDVK-GNKQLFQIYICLDNYAKEFVECPY 213

The following BLAST results are available for this feature:

BLAST of MC08g1412 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P23540	3.8e-32	35.57	Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P42813	2.6e-28	32.37	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
Q7M438	7.0e-26	31.94	Ribonuclease DdI OS=Dictyostelium discoideum OX=44689 GN=ddiA PE=1 SV=3	[more]
Q40966	5.0e-24	32.71	Ribonuclease S-4 OS=Pyrus pyrifolia OX=3767 PE=1 SV=2	[more]
P42815	1.1e-23	31.58	Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1	[more]

BLAST of MC08g1412 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158939.1	1.90e-175	100.00	ribonuclease MC-like [Momordica charantia]	[more]
XP_022156540.1	1.86e-107	66.52	ribonuclease MC-like [Momordica charantia]	[more]
XP_038903348.1	2.82e-97	60.18	ribonuclease MC-like [Benincasa hispida]	[more]
XP_022159354.1	1.94e-79	52.61	ribonuclease MC-like [Momordica charantia]	[more]
AEM42996.1	1.69e-74	49.35	S-RNase [Siraitia grosvenorii]	[more]

BLAST of MC08g1412 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E0X1	9.19e-176	100.00	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025395 PE=3 SV=1	[more]
A0A6J1DQK2	9.00e-108	66.52	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111023418 PE=3 SV=1	[more]
A0A6J1E266	9.38e-80	52.61	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025771 PE=3 SV=1	[more]
K7NBR4	8.19e-75	49.35	S-RNase OS=Siraitia grosvenorii OX=190515 PE=2 SV=1	[more]
A0A6J1D7Q5	1.12e-74	51.29	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111018138 PE=3 SV=1	[more]

BLAST of MC08g1412 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G02990.1	1.8e-29	32.37	ribonuclease 1	[more]
AT1G14220.1	2.2e-27	32.21	Ribonuclease T2 family protein	[more]
AT1G26820.1	7.9e-25	31.58	ribonuclease 3	[more]
AT1G14210.1	1.1e-23	28.91	Ribonuclease T2 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 25..216 e-value: 6.7E-50 score: 171.7
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 25..217
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 25..205 e-value: 3.3E-39 score: 135.0
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 11..208
None	No IPR available	PANTHER	PTHR11240:SF49	RIBONUCLEASE 3	coord: 11..208
IPR033130	Ribonuclease T2, His active site 2	PROSITE	PS00531	RNASE_T2_2	coord: 106..117
IPR033697	Ribonuclease T2, eukaryotic	CDD	cd01061	RNase_T2_euk	coord: 25..206 e-value: 9.92953E-56 score: 174.443

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g1412.1	MC08g1412.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding